CN116327799A - Preparation method of bletilla striata extract and application of bletilla striata extract in preparation of antibacterial products - Google Patents
Preparation method of bletilla striata extract and application of bletilla striata extract in preparation of antibacterial products Download PDFInfo
- Publication number
- CN116327799A CN116327799A CN202310334659.XA CN202310334659A CN116327799A CN 116327799 A CN116327799 A CN 116327799A CN 202310334659 A CN202310334659 A CN 202310334659A CN 116327799 A CN116327799 A CN 116327799A
- Authority
- CN
- China
- Prior art keywords
- bletilla striata
- extract
- preparation
- bletilla
- striata extract
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241001313857 Bletilla striata Species 0.000 title claims abstract description 82
- 239000000284 extract Substances 0.000 title claims abstract description 77
- 230000000844 anti-bacterial effect Effects 0.000 title claims abstract description 47
- 238000002360 preparation method Methods 0.000 title claims abstract description 47
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 76
- 238000000605 extraction Methods 0.000 claims abstract description 38
- 150000004676 glycans Chemical class 0.000 claims abstract description 25
- 229920001282 polysaccharide Polymers 0.000 claims abstract description 25
- 239000005017 polysaccharide Substances 0.000 claims abstract description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 24
- 239000000047 product Substances 0.000 claims abstract description 23
- 239000006228 supernatant Substances 0.000 claims abstract description 20
- 239000000843 powder Substances 0.000 claims abstract description 18
- 238000001556 precipitation Methods 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 30
- 238000001035 drying Methods 0.000 claims description 18
- 238000001816 cooling Methods 0.000 claims description 13
- 239000002244 precipitate Substances 0.000 claims description 13
- 239000007788 liquid Substances 0.000 claims description 10
- 238000000034 method Methods 0.000 claims description 8
- 241000588724 Escherichia coli Species 0.000 claims description 6
- 241000191967 Staphylococcus aureus Species 0.000 claims description 4
- 239000011259 mixed solution Substances 0.000 claims description 3
- 239000006071 cream Substances 0.000 claims description 2
- 239000008269 hand cream Substances 0.000 claims description 2
- 230000002401 inhibitory effect Effects 0.000 claims description 2
- 239000006210 lotion Substances 0.000 claims description 2
- 238000012423 maintenance Methods 0.000 claims description 2
- 241001313855 Bletilla Species 0.000 abstract description 41
- 241000196324 Embryophyta Species 0.000 abstract description 2
- 239000000203 mixture Substances 0.000 abstract 1
- 239000012153 distilled water Substances 0.000 description 18
- 238000006243 chemical reaction Methods 0.000 description 8
- 238000001914 filtration Methods 0.000 description 8
- 238000010438 heat treatment Methods 0.000 description 8
- 238000007789 sealing Methods 0.000 description 8
- 238000007873 sieving Methods 0.000 description 8
- 238000003756 stirring Methods 0.000 description 8
- 230000005764 inhibitory process Effects 0.000 description 5
- 229920001817 Agar Polymers 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 3
- 239000008272 agar Substances 0.000 description 3
- 230000000052 comparative effect Effects 0.000 description 3
- 238000004090 dissolution Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 2
- QAOWNCQODCNURD-UHFFFAOYSA-N Sulfuric acid Chemical compound OS(O)(=O)=O QAOWNCQODCNURD-UHFFFAOYSA-N 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- 239000004480 active ingredient Substances 0.000 description 2
- 239000013543 active substance Substances 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 239000008103 glucose Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 230000008569 process Effects 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- 238000012795 verification Methods 0.000 description 2
- ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 244000052616 bacterial pathogen Species 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 239000011248 coating agent Substances 0.000 description 1
- 238000000576 coating method Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000007865 diluting Methods 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000002474 experimental method Methods 0.000 description 1
- 230000002349 favourable effect Effects 0.000 description 1
- 239000000499 gel Substances 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000000144 pharmacologic effect Effects 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000005292 vacuum distillation Methods 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C08—ORGANIC MACROMOLECULAR COMPOUNDS; THEIR PREPARATION OR CHEMICAL WORKING-UP; COMPOSITIONS BASED THEREON
- C08B—POLYSACCHARIDES; DERIVATIVES THEREOF
- C08B37/00—Preparation of polysaccharides not provided for in groups C08B1/00 - C08B35/00; Derivatives thereof
- C08B37/0003—General processes for their isolation or fractionation, e.g. purification or extraction from biomass
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K31/00—Medicinal preparations containing organic active ingredients
- A61K31/70—Carbohydrates; Sugars; Derivatives thereof
- A61K31/715—Polysaccharides, i.e. having more than five saccharide radicals attached to each other by glycosidic linkages; Derivatives thereof, e.g. ethers, esters
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K36/00—Medicinal preparations of undetermined constitution containing material from algae, lichens, fungi or plants, or derivatives thereof, e.g. traditional herbal medicines
- A61K36/18—Magnoliophyta (angiosperms)
- A61K36/88—Liliopsida (monocotyledons)
- A61K36/898—Orchidaceae (Orchid family)
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/72—Cosmetics or similar toiletry preparations characterised by the composition containing organic macromolecular compounds
- A61K8/73—Polysaccharides
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
- A61P31/04—Antibacterial agents
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Veterinary Medicine (AREA)
- Public Health (AREA)
- Animal Behavior & Ethology (AREA)
- Natural Medicines & Medicinal Plants (AREA)
- Medicinal Chemistry (AREA)
- Epidemiology (AREA)
- Pharmacology & Pharmacy (AREA)
- Engineering & Computer Science (AREA)
- Mycology (AREA)
- Biotechnology (AREA)
- Birds (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Microbiology (AREA)
- Organic Chemistry (AREA)
- Molecular Biology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Alternative & Traditional Medicine (AREA)
- Medical Informatics (AREA)
- Materials Engineering (AREA)
- Sustainable Development (AREA)
- Communicable Diseases (AREA)
- Oncology (AREA)
- General Chemical & Material Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- Polymers & Plastics (AREA)
- Dermatology (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a preparation method of a bletilla striata extract and application of the bletilla striata extract in preparation of antibacterial products, and relates to the technical field of plant extraction. The preparation method comprises extracting the mixture of bletilla tuber powder and water at 60-80deg.C for 1-4 hr, concentrating the supernatant of the extracted product to obtain bletilla polysaccharide gum; and (3) carrying out alcohol precipitation on the bletilla striata polysaccharide gum to obtain a bletilla striata extract. Aiming at antibacterial performance, the invention optimizes the preparation process of the bletilla striata, improves the antibacterial efficacy of the bletilla striata polysaccharide under the condition of keeping the extraction rate and purity of the bletilla striata polysaccharide, and provides a way for preparing natural antibacterial products.
Description
Technical Field
The invention relates to the technical field of plant extraction, in particular to a preparation method of a bletilla striata extract and application of the bletilla striata extract in preparation of antibacterial products.
Background
The bletilla striata is a traditional Chinese medicine with pharmacological action, and in the tuber water extract, the bletilla striata polysaccharide is used as one of main active substances, so that the bletilla striata has remarkable medicinal value. The antibacterial efficacy, extraction efficiency and purity of the prepared bletilla striata extract are required to be improved.
In view of this, the present invention has been made.
Disclosure of Invention
The invention aims to provide a preparation method of a bletilla striata extract and application of the bletilla striata extract in preparation of antibacterial products.
The invention is realized in the following way:
in a first aspect, an embodiment of the present invention provides an application of a bletilla striata extract in preparing a product with an antibacterial effect, where the preparation method of the bletilla striata extract includes: extracting the mixed solution of the tuber powder of bletilla striata and water, and concentrating the supernatant of the extracted product to obtain bletilla striata polysaccharide gum; wherein the extraction temperature is 60-80 ℃ and the extraction time is 1-4 h; and (3) carrying out alcohol precipitation on the bletilla striata polysaccharide gum to obtain a bletilla striata extract.
In a second aspect, an embodiment of the present invention provides a method for preparing a bletilla striata extract, where the method is as described in the previous embodiment.
In a third aspect, the embodiment of the invention provides a product with antibacterial effect, which comprises the bletilla striata extract prepared by the preparation method in the previous embodiment.
The invention has the following beneficial effects:
the inventor optimizes the preparation process of the bletilla striata based on antibacterial performance, improves the antibacterial efficacy of the bletilla striata polysaccharide under the condition of keeping the extraction rate and purity of the bletilla striata polysaccharide, and provides a way for preparing natural antibacterial products.
Detailed Description
In order to make the objects, technical solutions and advantages of the embodiments of the present invention more clear, the technical solutions of the embodiments of the present invention will be clearly and completely described below. The specific conditions are not noted in the examples and are carried out according to conventional conditions or conditions recommended by the manufacturer. The reagents or apparatus used were conventional products commercially available without the manufacturer's attention.
Firstly, the embodiment of the invention provides an application of a bletilla striata extract in preparing a product with an antibacterial effect, and the preparation method of the bletilla striata extract comprises the following steps: extracting the mixed solution of the tuber powder of bletilla striata and water, and concentrating the supernatant of the extracted product to obtain bletilla striata polysaccharide gum; wherein the extraction temperature is 60-80 ℃ and the extraction time is 1-4 h; and (3) carrying out alcohol precipitation on the bletilla striata polysaccharide gum to obtain a bletilla striata extract.
Compared with the bletilla striata polysaccharide extracted by other processes, the preparation method can prepare the bletilla striata extract with excellent antibacterial effect under the condition of keeping good polysaccharide extraction rate and purity, and has the advantages of high preparation speed, low cost, easiness in implementation, good antibacterial effect and the like.
Optionally, the mesh number of the tuber powder of the bletilla striata is 100-400 mesh. Specifically, the range between any one or any two of 100, 150, 200, 250, 300, 350 and 400 mesh is possible.
Alternatively, the extracted temperature may be in a range between any one or any two of 60, 62, 64, 66, 68, 70, 72, 74, 76, 78, and 80.
Optionally, the extracting temperature is: 65-80 ℃.
Optionally, the extracting temperature is: 62-78 ℃. The temperature range can increase the solubility of active substances in rhizoma bletilla powder, so that the prepared rhizoma bletilla extract has high extraction rate and purity, and has better antibacterial effect.
Alternatively, the time of the extraction may be in a range between any one or any two of 1h, 2h, 3h, and 4 h.
Optionally, the extracting time is: 2-4 h, and suitable extraction time is favorable for fully dissolving the bletilla polysaccharide gum.
Optionally, the pressure of the extraction is normal pressure. The normal pressure is one atmosphere, which is the pressure of the gas generated by the atmosphere in normal life, and can be approximately regarded as one standard atmosphere, namely 100KPa or 0.1MPa. The process can ensure the dissolution of most of polysaccharide in the bletilla striata powder particles under normal pressure, is safer than pressurizing, has low requirements on equipment, and is easier to operate.
Alternatively, the number of extractions is at least 1 or more, such as 2-4. The number of times may specifically be any one or any range between two of 2 times, 3 times and 4 times.
Optionally, the ratio of the extracted feed liquid is (0.5-1.5): (10-30), wherein the feed liquid ratio is the volume ratio. The low feed-liquid ratio can cause the bletilla powder to form lump and influence the dissolution of polysaccharide gum; the high feed-to-liquid ratio can lead to the dissolution of other water-soluble substances, and more non-polysaccharide impurities are introduced.
The extracted feed ratio refers to a volume ratio between the extracted material and the solution (water), and specifically may be in a range between any one or any two of 0.5:10, 0.5:12, 0.5:14, 0.5:16, 0.5:18, 0.5:20, 0.5:22, 0.5:24, 0.5:26, 0.5:28, 0.5:30, 1:10, 1:12, 1:14, 1:16, 1:18, 1:20, 1:22, 1:24, 1:26, 1:28, 1:30, 1.5:10, 1.5:12, 1.5:14, 1.5:16, 1.5:18, 1.5:20, 1.5:22, 1.5:24, 1.5:26, 1.5:28, and 1.5:30.
Optionally, the feed liquid ratio is 1: (10-25).
Optionally, the alcohol precipitation includes: adding the bletilla striata polysaccharide gum into ethanol solution, and collecting precipitate.
Optionally, the concentration of the ethanol solution is 95% -100%. It will be appreciated that the concentration of the ethanol solution is the volume fraction of ethanol in the solution and may specifically be in the range between any one or any two of 95%, 96%, 97%, 98%, 99% and 100%.
Optionally, during alcohol precipitation, the volume ratio of the bletilla striata polysaccharide gum to the ethanol solution is 1: (1-4). The volume ratio may specifically be in a range between any one or any two of 1:1, 1:2, 1:3, and 1:4.
Optionally, the time of the alcohol precipitation is: 6-12h;
optionally, the ethanol solution is a precooled ethanol solution.
Optionally, the pre-cooling refers to pre-cooling to 4-8 ℃. The ethanol cooled to 4 ℃ in advance can rapidly precipitate the effective active ingredients in the bletilla striata powder, and finally the effective active ingredients are separated from other substances in a precipitation form.
Optionally, the preparation method further comprises: drying the precipitate after alcohol precipitation to obtain the bletilla striata extract.
Optionally, the antibacterial means: the bletilla striata extract has an inhibition effect on the growth of escherichia coli and staphylococcus aureus, and is shown that the growth of the escherichia coli and the staphylococcus aureus is inhibited within the range of coating the bletilla striata extract on an agar plate to form a inhibition zone, wherein the inhibition on the escherichia coli is stronger.
Optionally, the product is selected from: skin care and/or maintenance products. The skin care product comprises: any one of face cream, hand cream, lotion and hand leave-on gel.
The embodiment of the invention also provides a preparation method of the bletilla striata extract, which is the preparation method of the bletilla striata extract in any embodiment.
Optionally, the bletilla striata extract is a bletilla striata extract with antibacterial effect.
In addition, the embodiment of the invention also provides a product with an antibacterial effect, which comprises the bletilla striata extract prepared by the preparation method of any embodiment.
The features and capabilities of the present invention are described in further detail below in connection with the examples.
Example 1
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with 10 times of volume into the bletilla striata powder, and extracting for 4 hours at 80 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 20 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding 95% ethanol which is precooled to 4 ℃ with the volume 1 time of the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 2
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with 10 times of volume into the bletilla striata powder, and extracting for 2 hours at 80 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 10 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding pre-cooled 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 3
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with 10 times of volume into the bletilla striata powder, and extracting for 4 hours at 60 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 10 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding pre-cooled 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 4
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with 10 times of volume into the bletilla striata powder, and extracting for 2 hours at 60 ℃;
after the heating in the step (3) is finished, cooling to room temperature, filtering, and adding 20 times of distilled water into filter residues; continuously extracting for 1 time according to the reaction temperature and the reaction time in the step (2), and merging and concentrating the supernatant;
adding pre-cooled 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 5
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with the volume of 20 times to the bletilla striata powder, and extracting for 2 hours at 80 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 20 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding pre-cooled 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 6
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with the volume of 20 times to the bletilla striata powder, and extracting for 4 hours at 80 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 20 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding pre-cooled 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 7
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with the volume of 20 times to the bletilla striata powder, and extracting for 2 hours at the temperature of 60 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 20 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding cold 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 8
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps:
crushing tuber of bletilla striata, sieving with a 200-mesh sieve, sealing, drying and preserving in shade;
step (2) adding distilled water with the volume of 20 times to the bletilla striata powder, and extracting for 4 hours at the temperature of 60 ℃;
after the heating in the step (3), cooling to room temperature, filtering, adding distilled water with 20 times of volume into filter residues, continuously extracting for 1 time according to the reaction temperature and time in the step (2), and merging and concentrating supernatant;
adding cold 95% ethanol which is 4 times of the volume of the concentrated solution into the concentrated solution, stirring, standing overnight at 4 ℃, centrifuging, collecting precipitate, and recovering ethanol supernatant;
and (5) drying for 48 hours by a freeze dryer, and crushing to obtain the tuber extract of bletilla.
Example 9
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in step (2) was 75 ℃.
Example 10
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in the step (2) is 75 ℃ and 2 hours.
Example 11
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in the step (2) is 75 ℃ and 6 hours.
Example 12
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in step (2) was 70 ℃.
Example 13
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in the step (2) is 70 ℃ and 2 hours.
Example 14
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in the step (2) is 70 ℃ and 6 hours.
Example 15
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction in step (2) is performed in a vacuum distillation pot.
Example 16
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: and (4) replacing the precooled 95% ethanol with the normal-temperature 95% ethanol.
Example 17
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: and (3) pre-cooling 90% ethanol in the step (4).
Example 18
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the feed liquid ratio (volume ratio) in the extraction process of the step (2) is 1:15.
Example 19
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the feed liquid ratio (volume ratio) in the extraction process of the step (2) is 1:30.
Comparative example 1
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in the step (2) is 85 ℃ and the extraction time is 2h.
Comparative example 2
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in step (2) was 85 ℃.
Comparative example 3
A preparation method of rhizoma bletilla extract with antibacterial function comprises the following steps of: the extraction temperature in the step (2) is 85 ℃ and the extraction time is 6 hours.
Verification example 1
The crude polysaccharides of examples 1 to 8 were examined for extraction efficiency and purity.
Experimental procedure
Crude polysaccharide extraction (%) = extract dry weight (g)/bletilla tuber powder (g); purity (%) refers to the milligrams of sugar contained in 1g of extract, and the specific implementation steps include: weighing 50mg of glucose which is dried to constant weight at 105 ℃, adding 500 mL of distilled water to prepare 0.1mg/mL of standard solution, diluting the distilled water to 0,0.02,0.04,0.06,0.08 and 0.1mg/mL, then adding 300 mu L of 6% phenol and 1.5mL of sulfuric acid respectively, shaking uniformly, standing for 10 minutes, detecting absorbance at 490nm, and drawing a standard curve by taking glucose content as an abscissa and OD value as an ordinate. The sample was prepared at 1mg/mL, the above procedure was repeated, absorbance was measured at 490nm, and the total sugar content of the sample was calculated by taking the standard curve.
Experimental results
TABLE 1 extraction yield and purity
Verification example 2
The inhibition effect of the tuber extract of bletilla tuber prepared in example 1 on escherichia coli was verified.
Two common pathogenic bacteria of escherichia coli (ATCC 8739) and staphylococcus aureus (CICC 10384) are selected as test bacteria, and an agar diffusion method is adopted to diffuse the bletilla tuber extract in an agar plate, so that the bacterial growth around the bletilla tuber extract is inhibited to form a transparent circle, namely a bacteriostasis circle, and the bacteriostasis titer of the bletilla tuber extract to be tested is judged according to the size of the bacteriostasis circle. As can be seen from Table 2, the tuber extracts of bletilla striata obtained in examples 1 to 8 have inhibitory effects on 2 test bacteria.
TABLE 2 diameter of inhibition of samples (10 mg/mL) against 2 test bacteria
The above description is only of the preferred embodiments of the present invention and is not intended to limit the present invention, but various modifications and variations can be made to the present invention by those skilled in the art. Any modification, equivalent replacement, improvement, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
Claims (10)
1. The application of the bletilla striata extract in preparing a product with an antibacterial effect is characterized in that the preparation method of the bletilla striata extract comprises the following steps:
extracting the mixed solution of the tuber powder of bletilla striata and water, and concentrating the supernatant of the extracted product to obtain bletilla striata polysaccharide gum; wherein the extraction temperature is 60-80 ℃ and the extraction time is 1-4 h;
and (3) carrying out alcohol precipitation on the bletilla striata polysaccharide gum to obtain a bletilla striata extract.
2. The use according to claim 1, wherein the extraction temperature is: 65-80 ℃;
preferably, the extraction temperature is: 62-78 ℃;
preferably, the extraction time is: 2-4 h;
preferably, the pressure of the extraction is normal pressure.
3. The use according to claim 1, wherein the ratio of extracted feed to liquid is (0.5-1.5): (10-30), wherein the feed liquid ratio is the volume ratio;
preferably, the feed liquid ratio is 1: (10-25).
4. The use according to claim 1, wherein the alcohol precipitation comprises: adding the bletilla striata polysaccharide gum into ethanol solution, and collecting precipitate.
5. The use according to claim 4, wherein the concentration of the ethanol solution is 95-100%;
preferably, when alcohol precipitation is performed, the volume ratio of the bletilla striata polysaccharide gum to the ethanol solution is 1: (1-4);
preferably, the time of the alcohol precipitation is: 4-12 h;
preferably, the ethanol solution is a precooled ethanol solution;
preferably, the pre-cooling refers to pre-cooling to 4-8 ℃.
6. The use according to claim 5, wherein the preparation method further comprises: drying the precipitate after alcohol precipitation to obtain the bletilla striata extract.
7. The use according to any one of claims 1 to 6, wherein the antibacterial means: has inhibitory effect on at least one of Escherichia coli and Staphylococcus aureus.
8. Use according to any one of claims 1 to 6, wherein the product is selected from: skin care and/or maintenance products;
preferably, the skin care product comprises: any one of face cream, hand cream, lotion and hand leave-on gel.
9. A method for preparing a bletilla striata extract, characterized in that the method is a method for preparing a bletilla striata extract according to any one of claims 1-8;
preferably, the bletilla striata extract is a bletilla striata extract with antibacterial effect.
10. A product with antibacterial effect, which is characterized by comprising the bletilla striata extract prepared by the preparation method of claim 9.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310334659.XA CN116327799A (en) | 2023-03-31 | 2023-03-31 | Preparation method of bletilla striata extract and application of bletilla striata extract in preparation of antibacterial products |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310334659.XA CN116327799A (en) | 2023-03-31 | 2023-03-31 | Preparation method of bletilla striata extract and application of bletilla striata extract in preparation of antibacterial products |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN116327799A true CN116327799A (en) | 2023-06-27 |
Family
ID=86882134
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202310334659.XA Pending CN116327799A (en) | 2023-03-31 | 2023-03-31 | Preparation method of bletilla striata extract and application of bletilla striata extract in preparation of antibacterial products |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN116327799A (en) |
Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102453106A (en) * | 2010-10-20 | 2012-05-16 | 张安卉 | Preparation method of bletilla striata polysaccharide |
| CN103044560A (en) * | 2011-10-11 | 2013-04-17 | 欧阳靖云 | Preparation method of bletilla striata polysaccharide |
-
2023
- 2023-03-31 CN CN202310334659.XA patent/CN116327799A/en active Pending
Patent Citations (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102453106A (en) * | 2010-10-20 | 2012-05-16 | 张安卉 | Preparation method of bletilla striata polysaccharide |
| CN103044560A (en) * | 2011-10-11 | 2013-04-17 | 欧阳靖云 | Preparation method of bletilla striata polysaccharide |
Non-Patent Citations (1)
| Title |
|---|
| 罗玲丽，等: "白及多糖提取工艺及抑菌活性检测", 吉首大学学报(自然科学版), vol. 43, no. 5, 30 September 2022 (2022-09-30), pages 1 * |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| JPH09221484A (en) | Production of proanthocyanidin | |
| CN110201012B (en) | Preparation method and application of purslane extract | |
| CN111732622B (en) | Method for extracting hesperidin from immature bitter orange | |
| CN109651480A (en) | A method of separation momordica glycoside V | |
| CN110981921B (en) | Continuous method for synchronously extracting multiple effective components from figs | |
| CN115260334B (en) | Compound extraction process of mulberry leaf polysaccharide | |
| CN111499681B (en) | Method for extracting limonin | |
| NL2029555B1 (en) | Method for simultaneously extracting phytosterols and polysaccharides from pitaya stems | |
| CN110606900B (en) | Method for separating and purifying fructus Jujubae polysaccharide with antioxidant effect | |
| CN109320576A (en) | A kind of production method of high-content momordica grosvenori glycoside V | |
| CN111635440B (en) | Method for separating multiple active ingredients from immature bitter orange | |
| CN116327799A (en) | Preparation method of bletilla striata extract and application of bletilla striata extract in preparation of antibacterial products | |
| CN114890973B (en) | Method for extracting and separating water-soluble procyanidine in grape seeds | |
| CN114605368B (en) | Method for preparing luteolin ultrafine particles by using supercritical fluid enhanced solution dispersion technology | |
| CN110194810A (en) | A kind of grading purification method for the external glue polysaccharide of water shield improving hypoglycemic activity | |
| CN112442136A (en) | Method for extracting functional components from tremella | |
| CN111171111B (en) | Method for continuously extracting crude protein, polysaccharide and cellulose from fresh xanthoceras sorbifolia shells | |
| CN112321658B (en) | Method for extracting anthocyanin in aronia melanocarpa fruit | |
| CN114939084A (en) | Russule extract and preparation method and application thereof | |
| CN109851687B (en) | Method for separating and preparing fucoidin and algin from kelp | |
| CN110538205B (en) | Extraction method of tribuloside | |
| CN109666085B (en) | Method for purifying lemon peel pectin by alcohol precipitation | |
| CN110724206A (en) | Extraction method and application of usnea longissima polysaccharide | |
| CN116370517B (en) | Extraction method for extracting flavonoid substances from cranberries | |
| CN113461649B (en) | Method for extracting and purifying anthocyanin from edible calluses of thorn grape |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination |