CN116297378A - Light detection measuring system - Google Patents
Light detection measuring system Download PDFInfo
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- CN116297378A CN116297378A CN202310587214.2A CN202310587214A CN116297378A CN 116297378 A CN116297378 A CN 116297378A CN 202310587214 A CN202310587214 A CN 202310587214A CN 116297378 A CN116297378 A CN 116297378A
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- 238000001514 detection method Methods 0.000 title claims abstract description 111
- 230000005284 excitation Effects 0.000 claims abstract description 48
- 239000012295 chemical reaction liquid Substances 0.000 claims abstract description 44
- 238000005259 measurement Methods 0.000 claims abstract description 28
- 230000003287 optical effect Effects 0.000 claims description 19
- 230000005540 biological transmission Effects 0.000 claims description 4
- 230000000903 blocking effect Effects 0.000 description 9
- 238000003018 immunoassay Methods 0.000 description 5
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- 230000002238 attenuated effect Effects 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 238000000504 luminescence detection Methods 0.000 description 3
- 238000004020 luminiscence type Methods 0.000 description 3
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- MYMOFIZGZYHOMD-UHFFFAOYSA-N Dioxygen Chemical compound O=O MYMOFIZGZYHOMD-UHFFFAOYSA-N 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
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- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
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- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
- B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
- B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
- B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
- B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/531—Production of immunochemical test materials
- G01N33/532—Production of labelled immunochemicals
- G01N33/533—Production of labelled immunochemicals with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/58—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances
- G01N33/582—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving labelled substances with fluorescent label
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
- G01N2021/0106—General arrangement of respective parts
- G01N2021/0112—Apparatus in one mechanical, optical or electronic block
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
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Abstract
The present application relates to a photodetection measurement system. The light detection measurement system includes: the excitation light source is used for emitting a first light beam to the reaction liquid in the containing device, and the reaction liquid can emit a second light beam after being excited by the first light beam; light detection means for receiving the second light beam; a shutter device including a first shutter and a second shutter; the shutter device is provided with a first working position, a second working position and a third working position; when the shutter device is positioned at the first working position, the first shutter is in an open state, and the second shutter is in a closed state; when the shutter device is positioned at the second working position, the first shutter and the second shutter are simultaneously in a closed state so as to prevent the second light beam in the accommodating device from being dispersed to the outside; when the shutter device is positioned at the third working position, the second shutter is in an open state, and the first shutter is in a closed state, so that the light detection device can receive the second light beam. According to the scheme, the influence of the residual light after the excitation light is turned off on the detection result can be avoided.
Description
Technical Field
The application relates to the technical field of optical detection, in particular to a light detection measurement system.
Background
In chemiluminescent immunoassay and fluoroimmunoassay, the detection of the content and the content of a substance contained in a sample is achieved by detecting the luminescence intensity of a reaction solution of the sample to be detected. Because the luminescence of the reaction liquid belongs to the weak light category, in order to ensure that the detection system can accurately detect the luminescence intensity of the reaction liquid, the detection system and the device for containing the reaction liquid must be placed in a closed dark environment.
Because of the characteristics of chemiluminescence immunoassay and fluorescence immunoassay, the light intensity energy of the emitted excitation light is hundreds or even millions of times that of the detected light emitted by the reaction liquid, and after some excitation light sources of the related technologies emit the excitation light, only the laser emission is turned off electronically, the inventor finds that the electronic turn-off of the excitation light has delay, and the disappearance time of the residual light of the excitation light after the turn-off is long, which can generate certain interference on the luminescence detection and affect the detection accuracy.
Disclosure of Invention
In order to solve or partially solve the problems in the related art, the application provides a light detection measurement system, which can avoid the influence of residual light after the excitation light is turned off on a detection result.
The application provides a photodetection measurement system comprising: the excitation light source is used for emitting a first light beam to the reaction liquid in the containing device, and the reaction liquid can emit a second light beam after being excited by the first light beam; light detection means for receiving the second light beam; the shutter device comprises a first shutter for controlling the on-off of the light path between the excitation light source and the accommodating device and a second shutter for controlling the on-off of the light path between the light detection device and the accommodating device; the shutter device is provided with a first working position, a second working position and a third working position; when the shutter device is positioned at the first working position, the first shutter is in an open state, and the second shutter is in a closed state, so that the reaction liquid can be excited; when the shutter device is positioned at the second working position, the first shutter and the second shutter are simultaneously in a closed state so as to block the second light beam in the containing device from being dispersed to the outside; when the shutter device is located at the third working position, the second shutter is in an open state, and the first shutter is in a closed state, so that the light detection device can receive the second light beam.
Further, the holding device is positioned below the excitation light source, and the light detection device is positioned below the holding device;
the first shutter is located the top of holding the device, one side that first shutter is close to hold the device is equipped with the boss, the second shutter is located hold the below of device, the second shutter is close to one side of holding the device is equipped with the guide way.
Further, the first shutter comprises a first baffle plate, and a first light hole for the first light beam to pass through is formed in the first baffle plate; the second shutter comprises a second baffle plate, and a second light hole for the second light beam to pass through is formed in the second baffle plate; one side of the first shutter close to the containing device and one side of the second shutter close to the containing device are respectively provided with a frosted surface.
Further, the shutter device further comprises a connecting piece and a driving piece for driving the connecting piece to move, the connecting piece is respectively connected with the first baffle piece and the second baffle piece, and the driving piece drives the first baffle piece and the second baffle piece to be linked through the connecting piece;
when the reaction liquid is excited, the first light beam penetrates through the first light transmission hole to be injected into the reaction liquid, and the second baffle plate blocks light rays between the containing device and the light detection device from being transmitted; when the light detection device receives the second light beam, the driving piece enables the first baffle plate and the second baffle plate to be linked through the connecting piece, so that the first baffle plate can block light rays between the excitation light source and the containing device from being transmitted, and the second light beam emitted by the reaction liquid is received by the light detection device after passing through the second light holes.
Further, the light detection measurement system further comprises a first lens group positioned between the excitation light source and the containing device, a second lens group positioned between the containing device and the light detection device, and a first optical filter group positioned between the second lens group and the light detection device;
the first lens group is used for converging the first light beam emitted by the excitation light source into parallel light;
the second lens group is used for converging the second light beam emitted by the reaction liquid on the detection surface of the light detection device.
Further, the above-described light detection measurement system further includes a dichroic mirror and a standard light source that emits a third light beam toward the dichroic mirror, the dichroic mirror being obliquely arranged on an optical path between the excitation light source and the holding device, the dichroic mirror being configured to allow the first light beam to transmit therethrough while reflecting the third light beam, the third light beam being irradiated toward the light detection device and being receivable by the light detection device after being reflected by the dichroic mirror.
Further, the light detection and measurement system further comprises a third lens set positioned between the dichroic mirror and the standard light source, and a second filter set positioned between the third lens set and the standard light source.
Further, the wavelength of the first light beam is 650 nm-700 nm, and the wavelength of the second light beam is 600 nm-650 nm.
Further, the containing device is a microfluidic chip, one end of the microfluidic chip is provided with a reaction area, the reaction liquid is located in the reaction area, the upper side and the lower side of the reaction area are light-transmitting layers, and the side wall of the reaction area is light-proof.
Further, the reaction area is round or rectangular, and the reaction area is positioned between the first shutter and the second shutter.
The technical scheme that this application provided can include following beneficial effect: by arranging the shutter device, when the reaction liquid is excited, the shutter device is in a first working position, the first shutter is in an open state, a first light beam can be injected into the reaction liquid to excite the reaction liquid through the first shutter, and meanwhile, the second shutter is in a closed state, so that the first light beam entering the containing device is prevented from being injected into the light detection device again to damage the light detection device; after the reaction liquid is excited, the shutter device is switched to a second working position, the first shutter and the second shutter are simultaneously in a closed state, and the second light beam in the holding device is blocked from being dispersed to the outside, so that unnecessary fluorescent materials are attenuated, and fluorescence to be detected is collected; when measuring the second light beam, the shutter device switches to the third working position from the second working position, so that the second shutter is in an open state, the second light beam can be received by the light detection device after passing through the second shutter from the holding device, meanwhile, the first shutter is in a closed state, the light path propagation between the excitation light source and the holding device is cut off after the first shutter is closed, and the residual light of the first light beam after the excitation light source is closed is blocked from being injected into the holding device, so that the influence of the residual light of the first light beam on the detection result can be avoided.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
FIG. 1 is a schematic diagram of a light detection measurement system according to an embodiment of the present application;
FIG. 2 is a schematic structural view of a shutter device according to an embodiment of the present disclosure, where the shutter device is located at a first working position;
FIG. 3 is a top view of the shutter device shown in FIG. 2;
FIG. 4 is another top view of the shutter device shown in an embodiment of the present application, wherein the shutter device is in a second operational position;
FIG. 5 is another schematic view of a shutter device according to an embodiment of the present disclosure, where the shutter device is in a third operating position;
FIG. 6 is a top view of the shutter device shown in FIG. 5;
FIG. 7 is a schematic cross-sectional view of a shutter device shown in an embodiment of the present application;
fig. 8 is a schematic structural diagram of a microfluidic chip shown in an embodiment of the present application.
Reference numerals:
1-excitation light source, 2-first light beam, 3-second light beam, 4-light detection device, 5-first shutter, 51-first baffle, 52-first light hole, 53-boss, 6-second shutter, 61-second baffle, 62-second light hole, 63-guide slot, 7-connecting piece, 8-first lens group, 9-second lens group, 10-first filter group, 20-dichroic mirror, 30-standard light source, 40-third light beam, 50-third lens group, 60-second filter group, 70-holding device, 80-microfluidic chip, 81-reaction region, 82-interface layer, 83-flexible film layer, 84-fluid channel layer, 85-bottom plate layer.
Detailed Description
Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, a first message may also be referred to as a second message, and similarly, a second message may also be referred to as a first message, without departing from the scope of the present application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the description of the present application, it should be understood that the terms "length," "width," "upper," "lower," "front," "rear," "left," "right," "vertical," "horizontal," "top," "bottom," "inner," "outer," and the like indicate orientations or positional relationships based on the orientation or positional relationships shown in the drawings, merely to facilitate description of the present application and simplify the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and therefore should not be construed as limiting the present application.
Unless specifically stated or limited otherwise, the terms "mounted," "connected," "secured" and the like are to be construed broadly and may be, for example, fixedly connected or detachably connected or integrally formed; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communicated with the inside of two elements or the interaction relationship of the two elements. The specific meaning of the terms in this application will be understood by those of ordinary skill in the art as the case may be.
In the related art, due to the characteristics of chemiluminescence immunoassay and fluorescence immunoassay, the light intensity energy of the emitted excitation light is hundreds or even millions of times that of the detected light emitted by the reaction solution, the excitation light source only turns off the laser light on electrons after the excitation light is emitted, the inventor finds that the electron turning off the excitation light has delay, and the disappearance time of the residual light of the excitation light after the electron turning off is long, which can generate certain interference on the luminescence detection and influence the detection accuracy.
In view of the above problems, embodiments of the present application provide a light detection measurement system, which can avoid the influence of the residual light after the excitation light is turned off on the detection result.
The following describes the technical scheme of the embodiments of the present application in detail with reference to the accompanying drawings.
As shown in fig. 1, an embodiment of the present application provides a light detection measurement system, which includes an excitation light source 1, a light detection device 4, and a shutter device, and is used for performing luminescence detection on a reaction solution in a containing device 70.
The excitation light source 1 is used for emitting a first light beam 2 to the reaction liquid in the containing device 70, the excitation light source 1 can be a halogen lamp or an LED light source capable of emitting light with various wavelengths, the wavelength of the first light beam 2 is 650 nm-700 nm, preferably 685 nm, and the first light beam 2 is preferably red light.
The first light beam 2 irradiates the reaction liquid in the containing device 70, so that the donor microspheres in the reaction liquid generate singlet oxygen, and after the singlet oxygen reacts with the acceptor microspheres in the reaction liquid, the detection light with different wavelength from the first light beam 2, namely the second light beam 3, is emitted. The wavelength of the second light beam 3 is 600 nm-650 nm, preferably 615 nm.
The light detection device 4 is configured to receive the second light beam 3, detect the luminous intensity of the second light beam 3, and calculate the composition and content of the substance contained in the sample to be detected by measuring the light intensity of the second light beam 3 emitted by the reaction solution. The light detection means 4 may be a single photon counter (PMT), a side window photomultiplier, an end window photomultiplier, a silicon photocell, an optical camera, etc.
The shutter device comprises a first shutter 5 for controlling the on-off of the optical path between the excitation light source 1 and the containing device 70, and a second shutter 6 for controlling the on-off of the optical path between the light detection device 4 and the containing device 70; the shutter device has a first operating position, a second operating position, and a third operating position.
As shown in fig. 2 and 3, when the shutter device is in the first working position, the first shutter 5 is in an open state, the first light beam 2 can pass through the first shutter 5 and then be emitted to the reaction liquid, so that the reaction liquid can be excited, and meanwhile, the second shutter 6 is in a closed state, and the light in the containing device 70 is blocked by the second shutter 6 and cannot be emitted to the light detecting device 4. As shown in fig. 4, when the shutter device is located at the second working position, the first shutter 5 and the second shutter 6 are simultaneously in the closed state, so as to block the second light beam 3 in the containing device 70 from being diverged to the outside. As shown in fig. 5 and 6, when the shutter device is in the third operating position, the second shutter 6 is in an open state and the first shutter 5 is in a closed state, so that the light detecting device can receive the second light beam 3.
The holding device 70 may be configured to have a first light-transmitting region through which the first light beam 2 passes and a second light-transmitting region through which the second light beam 3 passes, wherein the first light beam 2 passes through the first light-transmitting region and then irradiates the reaction solution, and the second light beam 3 passes through the second light-transmitting region and then irradiates the light detection device 4. Other areas of the holding device 70 may be made of a material that is opaque or has a light reflecting function. The holding device 70 may be a microfluidic chip, and the structure of the holding device 70 may be designed according to practical needs, which is not limited herein.
By arranging the shutter device, when the reaction liquid is excited, the shutter device is in a first working position, the first shutter 5 is in an open state, the first light beam 2 can be injected into the reaction liquid to excite the reaction liquid through the first shutter 5, and meanwhile, the second shutter 6 is in a closed state, so that the first light beam 2 entering the containing device 70 is prevented from being injected to the light detection device 4 again to damage the light detection device 4; after the reaction liquid is excited, the shutter device is switched to a second working position, the first shutter and the second shutter are simultaneously in a closed state, and the second light beam 3 in the containing device 70 is blocked from being dispersed to the outside, so that unnecessary fluorescent materials are attenuated, and fluorescence to be detected is collected; when the second light beam 3 is measured, the shutter device is switched from the second working position to the third working position, so that the second shutter 6 is in an open state, the second light beam 3 can be received by the light detection device 4 after passing through the second shutter 6 from the holding device 70, meanwhile, the first shutter 5 is in a closed state, the light path transmission between the excitation light source 1 and the holding device 70 is cut off after the first shutter 5 is closed, and the residual light of the first light beam 2 after the excitation light source 1 is closed is blocked from entering the holding device 70, so that the influence of the residual light of the first light beam 2 on the detection result can be avoided.
In some embodiments, as shown in fig. 1, the holding device 70 is located below the excitation light source 1, the light detection device 4 is located below the holding device 70, and the light paths of the first light beam 2 and the second light beam 3 are both along the up-down direction, and the two light paths are not overlapped, so that conditions are provided for separately controlling one of the two shutters. As shown in fig. 1 and 7, the first shutter 5 is located above the holding device 70 and is used for controlling the on-off of the optical path between the excitation light source 1 and the holding device 70, a boss 53 is arranged on one side of the first shutter 5, which is close to the holding device 70, and the second shutter 6 is located below the holding device 70 and is used for controlling the on-off of the optical path between the holding device 70 and the light detection device 4, and a guide groove 63 is arranged on one side of the second shutter 6, which is close to the holding device 70. Wherein, the boss 53 can be in closer contact with the upper end of the containing device 70, and the guiding groove 63 can be in closer contact with the lower end of the containing device 70, thereby improving the light tightness of the containing device 70.
In some embodiments, as shown in fig. 1 to 7, the first shutter 5 includes a first blocking plate 51, a first light hole 52 through which the first light beam 2 passes is provided on the first blocking plate 51, when the excitation light source 1 excites the reaction liquid, the shutter device is located at a first working position, the first light beam 2 irradiates the reaction liquid after passing through the first light hole 52, when the shutter device is located at a second working position and a third working position, the first blocking plate 51 is driven to rotate, so that the first light hole 52 is moved to be staggered from the optical path of the first light beam 2, and the first blocking plate 51 blocks the residual light of the first light beam 2.
The second shutter 6 includes a second blocking piece 61, a second light hole 62 through which the second light beam 3 passes is provided on the second blocking piece 61, when the light detection device 4 detects, the shutter device is located at a third working position, the second light beam 3 passes through the second light hole 62 and irradiates onto the light detection device 4, and when the second working position and the first working position are used, the second blocking piece 61 is driven to rotate, so that the second light hole 62 is moved to be staggered with the light path of the second light beam 3, the second blocking piece 61 blocks the first light beam 2 injected into the reaction liquid to continuously downwards propagate, and the first light beam 2 is prevented from continuously downwards irradiating onto the light detection device 4, thereby preventing the light detection device 4 from being damaged by strong light irradiation.
The side of the first shutter 5 near the holding device 70 and the side of the second shutter 6 near the holding device 70 are respectively provided with frosted surfaces to form scattering reflection surfaces.
Specifically, the shutter device further includes a connecting piece 7 and a driving piece for driving the connecting piece 7 to move, the connecting piece 7 is respectively connected with the first baffle piece 51 and the second baffle piece 61, and the driving piece drives the first baffle piece 51 and the second baffle piece 61 to be linked through the connecting piece 7. The driving piece can be a motor, and the driving piece drives the first baffle plate 51 and the second baffle plate 61 to rotate simultaneously by driving the connecting piece 7 to enable the first baffle plate 51 and the second baffle plate 61 to be linked, so that the response speed of the two shutters is high.
When the reaction liquid is excited, as shown in fig. 2 and 3, the first light beam 2 passes through the first light hole 52 and enters the reaction liquid, and the second blocking piece 61 blocks the light between the holding device 70 and the light detecting device 4 from propagating, and the shutter device is in the first working position; when the light detection device 4 receives the second light beam 3, as shown in fig. 5 and 6, the driving piece makes the first baffle plate 51 and the second baffle plate 61 link through the connecting piece 7, so that the first baffle plate 51 blocks the light between the excitation light source 1 and the containing device 70 from propagating, and makes the second light beam 3 emitted by the reaction solution pass through the second light transmission hole 62 and then be received by the light detection device 4, and the shutter device is in the third working position; as shown in fig. 4, the second working position is located between the first working position and the third working position.
The first light beam 2 emitted by the excitation light source 1 is generally scattered, so as to achieve better optical performance and reduce energy loss, and the light detection measurement system of the embodiment of the present application further includes a first lens group 8 located between the excitation light source 1 and the containing device 70, where the first lens group 8 is used to collect the first light beam 2 emitted by the excitation light source 1 into parallel light. The first lens group 8 may be composed of a combination of lenses of different materials having various diameters and radii of curvature, and in the embodiment shown in fig. 1, the first lens group 8 comprises a single convex lens.
Further, since the second light beam 3 is scattered light when being emitted from the holding device 70, the light detection measurement system of the embodiment of the present application further includes a second lens group 9 located between the holding device 70 and the light detection device 4, and the second lens group 9 is used for converging the second light beam 3 emitted by the reaction solution on the detection surface of the light detection device 4. The second lens group 9 may be composed of a combination of lenses made of different materials having various diameters and radii of curvature. In the embodiment shown in fig. 1, the second lens group 9 comprises a single-sided convex lens.
As shown in fig. 1, the optical detection measurement system according to the embodiment of the present application further includes a first optical filter set 10 disposed between the second lens set 9 and the optical detection device 4, where the first optical filter set 10 may have a plurality of optical filters, and filters out light with unwanted interference wavelengths and impurity wavelengths, so that the light beams actually reflected by the reaction solution can be collected on the optical detection device 4 in a pure manner.
Furthermore, due to the specificity of the detection principle in immunity and the characteristics of the light detection device 4, the output of the light detection device 4 can drift along with the extension of the service time and the change of the ambient temperature, thereby affecting the accuracy of the detection result.
In order to solve the problem that the output of the light detection device 4 may drift and further affect the accuracy of the detection result, as shown in fig. 1, the light detection measurement system according to the embodiment of the present application further includes a dichroic mirror 20 and a standard light source 30 emitting a third light beam 40 toward the dichroic mirror 20, where the dichroic mirror 20 is obliquely arranged on the light path between the excitation light source 1 and the containing device 70, and the angle of inclination of the dichroic mirror 20 may be 45 °; the dichroic mirror 20 is configured to allow the first light beam 2 to pass through and reflect the third light beam 40, and the third light beam 40 is irradiated towards the light detection device 4 and can be received by the light detection device 4 after being reflected by the dichroic mirror 20.
Specifically, the third light beam 40 is used to calibrate the accuracy and linearity of the measurement of the light detection device 4, and the power of the standard light source 30 is adjusted by a circuit to check whether the detection value of the light detection device 4 is accurate or not and whether the detection value is linear or not.
In some embodiments, as shown in fig. 1, the light detection measurement system further includes a third lens group 50 positioned between the dichroic mirror 20 and the standard light source 30, and a second filter group 60 positioned between the third lens group 50 and the standard light source 30. The third lens group 50 is configured to collect the third light beam 40 into parallel light, and may include a convex lens. The second filter set 60 may include a plurality of filters to filter out light with unwanted interference wavelengths and wavelengths to obtain the third light beam 40 with the desired wavelength.
In one possible implementation, the optical path detection timing at the time of detection is as follows: the shutter device is located at a first working position, the excitation light source 1 excites the reaction liquid for a certain time, for example, 300ms, after the reaction liquid is excited to emit light, a second light beam 3 with 615nm is generated, wherein the reaction time after excitation has a certain requirement, the excitation is performed for 600ms, then the shutter device is located at a second working position, waiting for 200ms, so that unnecessary fluorescent substances are attenuated, and then the shutter device is located at a third working position, and the required detection fluorescence is collected.
In some embodiments, as shown in fig. 8, the holding device 70 is a microfluidic chip 80, one end of the microfluidic chip 80 is provided with a reaction zone 81, the reaction liquid is located in the reaction zone 81, the upper side and the lower side of the reaction zone 81 are light-transmitting layers, and the side wall of the reaction zone 81 is light-proof.
Specifically, the microfluidic chip 80 includes an interface layer 82, a flexible film layer 83, a fluid channel layer 84, and a bottom layer 85 stacked in sequence from top to bottom, and the reaction region 81 is disposed on the fluid channel layer 84. In the microfluidic chip 80, the side wall of the reaction region 81 or the whole microfluidic chip 80 is provided with an opaque layer except for the upper and lower side regions of the reaction region 81.
Alternatively, the reaction zone 81 is circular or rectangular, and the reaction zone 81 is located between the first shutter 5 and the second shutter 6.
In summary, the embodiment of the application provides a light detection measurement system, which can avoid the influence of the residual light after the excitation light is turned off on the detection result, and calibrate the light detection device 4 through the standard light source 30, so that the problem that the output of the light detection device 4 can drift and further influence the accuracy of the detection result is solved.
The aspects of the present application have been described in detail hereinabove with reference to the accompanying drawings. In the foregoing embodiments, the descriptions of the embodiments are focused on, and for those portions of one embodiment that are not described in detail, reference may be made to the related descriptions of other embodiments. Those skilled in the art will also appreciate that the acts and modules referred to in the specification are not necessarily required in the present application. In addition, it can be understood that the steps in the method of the embodiment of the present application may be sequentially adjusted, combined and pruned according to actual needs, and the modules in the apparatus of the embodiment of the present application may be combined, divided and pruned according to actual needs.
The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. A light detection measurement system, comprising:
the excitation light source is used for emitting a first light beam to the reaction liquid in the containing device, and the reaction liquid can emit a second light beam after being excited by the first light beam;
light detection means for receiving the second light beam; and
the shutter device comprises a first shutter for controlling the on-off of a light path between the excitation light source and the accommodating device and a second shutter for controlling the on-off of a light path between the light detection device and the accommodating device; the shutter device is provided with a first working position, a second working position and a third working position; when the shutter device is positioned at the first working position, the first shutter is in an open state, and the second shutter is in a closed state, so that the reaction liquid can be excited; when the shutter device is positioned at the second working position, the first shutter and the second shutter are simultaneously in a closed state so as to block the second light beam in the containing device from being dispersed to the outside; when the shutter device is located at the third working position, the second shutter is in an open state, and the first shutter is in a closed state, so that the light detection device can receive the second light beam.
2. The light detection measurement system of claim 1, wherein:
the holding device is positioned below the excitation light source, and the light detection device is positioned below the holding device;
the first shutter is located the top of holding the device, one side that first shutter is close to hold the device is equipped with the boss, the second shutter is located hold the below of device, the second shutter is close to one side of holding the device is equipped with the guide way.
3. The light detection measurement system of claim 1, wherein:
the first shutter comprises a first baffle plate, and a first light hole for the first light beam to pass through is formed in the first baffle plate; the second shutter comprises a second baffle plate, and a second light hole for the second light beam to pass through is formed in the second baffle plate; one side of the first shutter close to the containing device and one side of the second shutter close to the containing device are respectively provided with a frosted surface.
4. A light detection measurement system according to claim 3, wherein:
the shutter device further comprises a connecting sheet and a driving piece for driving the connecting sheet to move, the connecting sheet is respectively connected with the first baffle sheet and the second baffle sheet, and the driving piece drives the first baffle sheet and the second baffle sheet to be linked through the connecting sheet;
when the reaction liquid is excited, the first light beam penetrates through the first light transmission hole to be injected into the reaction liquid, and the second baffle plate blocks light rays between the containing device and the light detection device from being transmitted; when the light detection device receives the second light beam, the driving piece enables the first baffle plate and the second baffle plate to be linked through the connecting piece, so that the first baffle plate can block light rays between the excitation light source and the containing device from being transmitted, and the second light beam emitted by the reaction liquid is received by the light detection device after passing through the second light holes.
5. The light detection measurement system of claim 1, wherein:
the light source device further comprises a first lens group positioned between the excitation light source and the containing device, a second lens group positioned between the containing device and the light detection device, and a first optical filter group positioned between the second lens group and the light detection device;
the first lens group is used for converging the first light beam emitted by the excitation light source into parallel light;
the second lens group is used for converging the second light beam emitted by the reaction liquid on the detection surface of the light detection device.
6. The light detection measurement system of claim 1, wherein:
and a dichroic mirror and a standard light source emitting a third light beam toward the dichroic mirror, the dichroic mirror being obliquely arranged on an optical path between the excitation light source and the holding means, the dichroic mirror being configured to allow the first light beam to transmit therethrough while reflecting the third light beam, the third light beam being irradiated toward the light detection means and being receivable by the light detection means after being reflected by the dichroic mirror.
7. The light detection measurement system of claim 6, wherein:
also included is a third lens set between the dichroic mirror and the standard light source, and a second filter set between the third lens set and the standard light source.
8. The light detection measurement system of any one of claims 1-7, wherein:
the wavelength of the first light beam is 650 nm-700 nm, and the wavelength of the second light beam is 600 nm-650 nm.
9. The light detection measurement system of claim 1, wherein:
the device is a microfluidic chip, one end of the microfluidic chip is provided with a reaction zone, the reaction liquid is positioned in the reaction zone, the upper side and the lower side of the reaction zone are light-transmitting layers, and the side wall of the reaction zone is light-proof.
10. The light detection measurement system of claim 9, wherein:
the reaction zone is circular or rectangular, and the reaction zone is positioned between the first shutter and the second shutter.
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