CN116519673B - Light excitation chemiluminescence detection device, method and light excitation chemiluminescence detector - Google Patents
Light excitation chemiluminescence detection device, method and light excitation chemiluminescence detector Download PDFInfo
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- CN116519673B CN116519673B CN202310808476.7A CN202310808476A CN116519673B CN 116519673 B CN116519673 B CN 116519673B CN 202310808476 A CN202310808476 A CN 202310808476A CN 116519673 B CN116519673 B CN 116519673B
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- 230000005284 excitation Effects 0.000 title claims abstract description 69
- 238000000034 method Methods 0.000 title claims abstract description 16
- 238000002038 chemiluminescence detection Methods 0.000 title description 14
- 238000001514 detection method Methods 0.000 claims abstract description 59
- 238000006243 chemical reaction Methods 0.000 claims description 15
- 239000000463 material Substances 0.000 claims description 14
- 230000007246 mechanism Effects 0.000 claims description 9
- 230000001443 photoexcitation Effects 0.000 claims description 9
- 238000007789 sealing Methods 0.000 claims description 9
- 238000006552 photochemical reaction Methods 0.000 claims description 8
- 239000012295 chemical reaction liquid Substances 0.000 abstract description 7
- 230000003287 optical effect Effects 0.000 description 6
- 238000004020 luminiscence type Methods 0.000 description 5
- 230000000903 blocking effect Effects 0.000 description 4
- 238000010586 diagram Methods 0.000 description 3
- 238000001914 filtration Methods 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 230000007547 defect Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000003018 immunoassay Methods 0.000 description 2
- 238000002834 transmittance Methods 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000003111 delayed effect Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
- 239000010703 silicon Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
Classifications
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6402—Atomic fluorescence; Laser induced fluorescence
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- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
Abstract
The application relates to a light excitation chemiluminescence detector, a light excitation chemiluminescence detector method and a light excitation chemiluminescence detector. The device comprises a sample storage part for storing a detected sample; the light source module is provided with an excitation light path between the light source module and the sample storage part, and projects excitation light to the sample storage part through the excitation light path; the signal light collecting piece, the sample storage part is installed in the signal light collecting piece, a cavity is formed in the signal light collecting piece, the sample storage part is at least partially accommodated in the cavity, the inner wall of the cavity is a reflecting surface, the signal light collecting piece is provided with a light outlet communicated with the cavity, and the reflecting surface is used for converging signal light generated by a sample to the light outlet; the detector is arranged at the light outlet, a detection light path is arranged between the light outlet and the detector, and the detector is used for receiving the signal light guided out from the light outlet through the detection light path. According to the scheme provided by the application, the luminous intensity of the reaction liquid can be detected more accurately.
Description
Technical Field
The present application relates to the field of chemiluminescent immunoassay technology, and in particular, to a device and method for detecting chemiluminescence by using a light-activated chemiluminescent detector.
Background
In chemiluminescent immunoassay and fluoroimmunoassay, the detection of the content and the content of a substance contained in a sample is achieved by detecting the luminescence intensity of a reaction solution of the sample to be detected. Because the luminescence of the reaction liquid belongs to the weak light category, the detection system needs to be ensured to accurately detect the luminescence intensity of the detected sample.
In the related art, a complex lens or a spherical lens with multiple incident planes being planes is generally adopted to collect fluorescent signals emitted by a tested sample, and because the fluorescent signals are very weak, the detection system in the related art has the defects of large loss of light detection signals and loss of different signal quantities in different directions, so that the detection system is difficult to accurately detect the luminous intensity of the reaction liquid.
Disclosure of Invention
In order to solve or partially solve the problems in the related art, the present application provides a light-activated chemiluminescence detection device, a method and a light-activated chemiluminescence detector, which can detect the luminescence intensity of a reaction solution more accurately.
A first aspect of the present application provides a photoexcitation chemiluminescent detection device, comprising:
a sample storage section for storing a sample to be detected;
a light source module, an excitation light path is arranged between the light source module and the sample storage part, and the light source module projects excitation light to the sample storage part through the excitation light path;
the signal light collecting part is arranged on the signal light collecting part, a cavity is formed in the signal light collecting part, the sample storing part is at least partially accommodated in the cavity, the inner wall of the cavity is a reflecting surface, the signal light collecting part is provided with a light outlet communicated with the cavity, and the reflecting surface is used for converging signal light generated by the sample to the light outlet;
the detector is arranged at the light outlet, a detection light path is arranged between the light outlet and the detector, and the detector is used for receiving the signal light guided out from the light outlet through the detection light path.
In one embodiment, the signal light collecting member has a spherical structure, the spherical surface inside the spherical structure is the reflecting surface, and the sample storage part is disposed near the center inside the spherical structure.
In one embodiment, the signal light collecting member is made of an opaque material, and a portion of the sample storage portion located inside the signal light collecting member is made of a light transmissive material; alternatively, the signal light collecting member is coated with an opaque material, and the portion of the sample storage portion located in the signal light collecting member is made of an opaque material.
In one embodiment, a first shutter assembly is disposed between the light source module and the sample storage portion, and is used for switching on or off the excitation light path;
the second shutter assembly is arranged between the light outlet and the detector and is used for switching on or switching off the detection light path;
a controller electrically connected to the first shutter assembly and the second shutter assembly for controlling the first shutter assembly to open and the second shutter assembly to close during a first period of time; or, when the first shutter assembly is controlled to be closed in a second period, the second shutter assembly is opened.
In one embodiment, the first shutter assembly and the second shutter assembly are connected through a linkage mechanism, and when the first shutter assembly is opened, the linkage mechanism drives the second shutter assembly to be closed; when the first shutter assembly is closed, the second shutter assembly is driven to be opened through the linkage mechanism; or,
the first shutter assembly and the second shutter assembly are opened or closed according to a preset time sequence.
In one embodiment, the signal light collecting member is provided with a mounting port, the sample storage part is inserted into the signal light collecting member from the mounting port, and the light outlet is arranged at one end of the signal light collecting member away from the mounting port;
an opening is formed in one side, facing the light source module, of the sample storage part, and a light-sealing structure is arranged at the opening.
In one embodiment, the light source module, the sample storage part, the signal light collecting member and the detector are arranged along a central line of the signal light collecting member, and the mounting port and the light outlet are respectively arranged at two ends of the signal light collecting member on the central line.
In one embodiment, an opening is formed in a side, facing the light source module, of the sample storage portion, and a light-sealing structure is arranged at the opening.
A second aspect of the present application provides a photo-activated chemiluminescent detector comprising a light detection device as described in the first aspect above.
A third aspect of the present application provides a light-activated chemiluminescence detection method, including:
placing a sample storage part with a sample on a signal light collecting piece;
controlling a light source module to project excitation light to the sample storage part, so that the sample is subjected to photochemical reaction under the excitation of the excitation light; the signal light collecting piece is provided with a light outlet communicated with the cavity, and the reflecting surface is used for converging signal light generated by the sample to the light outlet;
after the reaction is completed, the detector receives and detects the signal light emitted by the sample in the sample storage part through the light outlet.
In one embodiment, the controlling the light source module to project excitation light to the sample storage unit to make the sample perform photochemical reaction under excitation of the excitation light includes:
controlling the first shutter assembly to be opened so that excitation light emitted by the light source module is projected into the sample storage part through an excitation light path; when the first shutter assembly is opened for a preset time period, the first shutter assembly is controlled to be closed, so that the sample storage part is in a light-tight environment, and the sample is subjected to photochemical reaction under the excitation of excitation light; or alternatively, the first and second heat exchangers may be,
after the reaction is completed, the detector receives and detects the signal light emitted by the sample in the sample storage part through the light outlet, and the signal light comprises:
when the reaction is completed, the second shutter assembly is opened at a predetermined timing so that the detector receives and detects the signal light emitted from the sample in the sample storage section through the light outlet.
The technical scheme that this application provided can include following beneficial effect:
according to the photo-excitation chemiluminescence detection device provided by the embodiment, the sample storage part is at least partially accommodated in the cavity, the inner wall of the cavity is the reflecting surface, after the light source module projects laser light to the sample, fluorescent signals generated by the sample can be converged at the light outlet under the reflection of the reflecting surface, the detector can detect reflected light led out after light is emitted, and in the process of light detection, the loss of light detection signals is small, so that the detector can accurately detect the luminous intensity of the reaction liquid.
In the light-activated chemiluminescence detection device provided by the embodiment, after the light source module is turned off, residual light exists in a short time, and the residual light of the light source module can influence the detection value of the detector because the power of the light source module is higher than that of the detector. In this embodiment, after first shutter subassembly was opened, can make and form the isolation between light source module and the sample storage portion, after first shutter subassembly was closed, open the second shutter subassembly simultaneously, when guaranteeing that the detector received the signal light, can not receive the interference of the surplus light of excitation light, promoted the detection precision of signal light, protection detector is not damaged.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
FIG. 1 is a schematic diagram of a photo-activated chemiluminescent detection device according to an embodiment of the present application;
FIG. 2 is a schematic diagram of a photo-activated chemiluminescent detection device according to another embodiment of the present application;
FIG. 3 is a flow chart of a photo-activated chemiluminescence detection method according to an embodiment of the disclosure;
fig. 4 is a flow chart of a photo-activated chemiluminescence detection method according to another embodiment of the disclosure.
Reference numerals: 100. a light source module; 200. a sample storage unit; 210. an opening; 220. an object to be detected; 300. a signal light collecting member; 310. a mounting port; 320. a light outlet; 330. a cavity; 400. a detector; 410. detecting a lens; 500. a first shutter assembly; 600. a first lens group; 700. a second lens group; 800. a second shutter assembly.
Detailed Description
Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, a first message may also be referred to as a second message, and similarly, a second message may also be referred to as a first message, without departing from the scope of the present application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the related art, a complex lens or a spherical lens with multiple incident planes being planes is generally adopted to collect fluorescent signals emitted by a tested sample, and because the fluorescent signals are very weak, the detection system in the related art has the defects of large loss of light detection signals and loss of different signal quantities in different directions, so that the detection system is difficult to accurately detect the luminous intensity of the reaction liquid.
In view of the above, embodiments of the present application provide a photoexcitation chemiluminescent detection device capable of detecting the luminescence intensity of a reaction solution more accurately.
The following describes the technical scheme of the embodiments of the present application in detail with reference to the accompanying drawings.
Fig. 1 is a schematic structural diagram of a photo-excitation chemiluminescent detection device according to an embodiment of the present application.
Referring to fig. 1, the light detection device of the embodiment of the present application includes a sample storage section 200 for storing a sample to be detected, which may be a reaction liquid; a light source module 100 having an excitation light path between the light source module 100 and the sample storage part 200, the light source module 100 projecting excitation light to the sample storage part 200 through the excitation light path; the signal light collecting member 300, the sample storage part 200 is mounted on the signal light collecting member 300, a cavity 330 is formed in the signal light collecting member 300, the sample storage part 200 is at least partially accommodated in the cavity 330, the inner wall of the cavity 330 is a reflecting surface, the signal light collecting member 300 is provided with a light outlet communicated with the cavity 330, and the reflecting surface is used for converging signal light generated by a sample to the light outlet 320; the detector 400 is disposed at the light outlet 320, a detection light path is provided between the light outlet 320 and the detector 400, and the detector 400 is configured to receive the signal light guided from the light outlet through the detection light path.
According to the scheme provided by the embodiment, the sample storage part 200 is at least partially accommodated in the cavity 330, the inner wall of the cavity 330 is the reflecting surface, after the light source module 100 projects laser to the sample, fluorescent signals generated by the sample can be converged at the light outlet under the reflection of the reflecting surface, the detector 400 can detect reflected light led out from the light outlet, and in the light detection process, the light detection signal loss is small, so that the detector can more accurately detect the luminous intensity of the reaction liquid. The sample of the present embodiment may be a liquid to be measured, also referred to as a sample.
In this embodiment, the light source module 100 may be a laser emitter, and the wavelength range of the excitation light emitted by the light source module 100 may be 675nm to 685nm. After the object to be detected 220 is excited, light with a wavelength of about 615nm can be generated through reaction, the light with a wavelength of 615nm can be converged at the light outlet 320 after being optically processed by the signal light collecting element 300, a detection lens 410 for receiving signal light is arranged at one side of the detector 400 opposite to the light outlet 320, and the detector 400 can convert the received light signal into an electric signal for an analyzer to detect the concentration of the object to be detected 220 through the electric signal.
In some embodiments, detector 400 may be one of a single photon counter, a silicon photocell, an integrating sphere, a photomultiplier tube.
In some embodiments, the signal light collecting member 300 has a spherical structure, the spherical surface inside the spherical structure is a reflecting surface, and the sample storage part 200 is disposed near the center inside the spherical structure. After the fluorescent signal emitted from the sample is emitted in all directions around the sample, the fluorescent signal can be reflected by the spherical surface and converged at the light outlet 320.
Because the fluorescent signal emitted by the sample is very weak, in the related art, some detection systems are excited by the upper opening of the reaction cup and then receive the light detection signal through diffuse reflection of the inner wall of the reaction cup; other detection systems are excited through the side face of the reaction cup, and light detection signals are received on the other side face, and the detection methods have large light detection signal loss and different signal quantity losses in all directions. In this embodiment, since the reflecting surface around the sample is spherical and the sample is located near the center of the spherical structure, there is no loss of optical signal in all directions around the sample, so that the light guided out by the light outlet is ensured to have enough luminous intensity, and the detection accuracy of the detection system is higher.
In some embodiments, the signal light collecting element 300 is made of a light-impermeable material, the signal light collecting element 300 is coated with a light-impermeable material, and the sample storage portion 200 is made of a light-permeable material, for example, the sample storage portion 200 may be a transparent cup with high transmittance. In the detection process, the optical signals emitted by the sample can be basically transmitted out from the sample storage part 200, and meanwhile, the optical signals cannot be transmitted out from the signal light collecting part 300, so that the light emitted by the sample can be basically reflected to the light outlet 320, and the loss of the optical signals is avoided.
In some embodiments, an opening 210 is disposed at a side of the sample storage portion 200 facing the light source module 100, the opening 210 is used for the laser emitted by the light source module 100 to project onto the sample, and a light-sealing structure is disposed at the opening 210, and the light-sealing structure can seal the opening 210 to ensure the light-sealing property of the signal collecting member or the sample storage portion 200.
In one implementation, the light-dense structure may employ a notch-boss interface structure. It should be understood that the light-sealing structure may be other structures capable of sealing the sample storage portion 200, and the present embodiment is not limited thereto.
In some embodiments, a first lens group 600 is disposed between the light source module 100 and the sample storage portion 200, and the first lens group 600 is used for filtering or collimating the excitation light emitted by the light source module 100 and then transmitting the excitation light to the sample storage portion 200 along an excitation light path.
In some embodiments, a second lens group 700 is disposed between the light outlet 320 and the detector 400, and the second lens group 700 includes a filtering lens, where the filtering lens is used to filter the signal light led out from the light outlet 320, filter the light with stray light and interference wavelength, and finally project the signal light with specific wavelength actually generated by the object to be detected 220 onto the detector 400, thereby further improving the detection accuracy.
In some embodiments, the second lens group 700 may further include a focusing lens, and may focus the signal light guided out from the light outlet 320 into a light spot and then project the light spot onto the receiving lens of the detector 400.
In some embodiments, the light-sealing structure includes a light-blocking member, the light-blocking member is used for covering the opening 210, the light-blocking member is opaque, a boss is disposed on a side of the light-blocking member facing the opening 210, and the boss can be matched with the opening 210 in a concave-convex manner to block the opening 210. In this embodiment, the light blocking member is in transmission connection with the driving member, the driving member can drive the light blocking member to cover or separate from the opening 210, and when the sample needs to be excited, the driving member drives the light blocking member to separate from the opening 210; after the excitation is completed, the driving member drives the light blocking member to cover the opening 210, so that the sample storage portion 200 keeps light-tight.
In some embodiments, the signal light collecting member 300 is provided with a mounting opening 310, the sample storage portion 200 is inserted into the signal light collecting member 300 from the mounting opening 310, and the light outlet 320 is disposed at an end of the signal light collecting member 300 away from the mounting opening 310. In this embodiment, the light source module 100, the sample storage 200, the signal light collecting member 300, and the detector 400 are arranged along the center line L of the signal light collecting member 300, and the mounting port 310 and the light outlet 320 are provided at both ends of the center line L of the signal light collecting member 300 in the vertical direction. After such arrangement, the distance between the sample storage portion 200 and the signal light collecting element 300 is the same in each direction of the same horizontal height, and the signal light is reflected by the reflecting surface one or more times in different directions, and then can be collected at the light outlet 320, and further received by the detector 400.
In some embodiments, the light source module 100, the sample storage portion 200, the light outlet 320 and the receiver are disposed along the same straight line in the vertical direction, and the straight line is the central axis of the signal light collecting member 300.
In some embodiments, the signal light collecting element 300 is provided with a supporting portion fixed relative to the signal light collecting element 300, and when the sample storage portion 200 is placed in the signal light collecting element 300 from the mounting opening 310, the supporting portion limits the sample storage portion 200, so that the sample storage portion 200 and the signal light collecting element 300 can be ensured to be relatively stable.
In some embodiments, the sample storage portion 200 includes a main body and an opening 210 disposed at an upper end of the main body, the main body is used for accommodating a sample, an outer diameter of the main body is slightly smaller than an inner diameter of the mounting opening 310, and the outer diameter of the opening 210 is larger than the inner diameter of the mounting opening 310, after the sample storage portion 200 is inserted into the mounting opening 310, an edge of the opening 210 can abut against an edge of the mounting opening 310, so as to limit the sample storage portion 200, so that the sample storage portion 200 and the signal light collecting member 300 can be ensured to be relatively stable, and in addition, the edge of the opening 210 of the sample storage portion 200 can be blocked to enable the mounting opening 310 of the signal light collecting member 300, so that the light tightness inside the signal light collecting member 300 is ensured.
Referring to fig. 2, in some embodiments, the photo-activated chemiluminescence detection apparatus further includes a first shutter assembly 500 disposed between the light source module 100 and the sample storage portion 200, for switching on or off an excitation light path; the second shutter assembly 800 is disposed between the light outlet 320 and the detector 400, and is used for switching on or switching off a detection light path; a controller electrically connected to the first shutter assembly 500 and the second shutter assembly 800 for controlling the second shutter assembly 800 to be closed when the first shutter assembly 500 is opened during a first period of time; or, the second shutter assembly 800 is opened when the first shutter assembly 500 is controlled to be closed during the second period.
The first shutter assembly 500 and the second shutter assembly 800 of the present embodiment may be any structure capable of achieving on-off of an optical path, and the first shutter assembly 500 and the second shutter assembly 800 may be shutters in the related art, for example, shutters similar to cameras.
The controller is electrically connected to the first shutter assembly 500 and the second shutter assembly 800, and can control the first shutter assembly 500 and the second shutter assembly 800 to be opened or closed at a preset time sequence, so as to conduct or cut off the excitation light path and the detection light path in different time periods, so as to realize multiple excitation of the sample by the excitation light emitted by the light source module 100, and receive the signal light generated by the sample by the detector 400 after each excitation.
When the light source module is excited, the controller controls the first shutter assembly 500 to be opened, so that an excitation light path between the light source module 100 and the sample storage portion 200 is conducted, a light beam emitted by the light source module 100 can irradiate the sample in the sample storage portion 200 through the excitation light path, and excitation is performed on the sample for a set period of time, for example, 300ms can be excited by the excitation light of the light source module 100. After the excitation is completed, the first shutter assembly 500 is controlled to be closed, at this time, the excitation light path is cut off, and the second shutter assembly 800 corresponding to the detector 400 is controlled to be opened after a set period of time (for example, 200 ms) is delayed, so that the detection light path is turned on, and the detector 400 receives the signal light emitted from the light outlet 320 through the detection light path.
In some embodiments, the first shutter assembly 500 and the second shutter assembly 800 are connected by a linkage mechanism, and when the first shutter assembly 500 is opened, the linkage mechanism drives the second shutter assembly 800 to close; when the first shutter assembly 500 is closed, the second shutter assembly 800 is driven to open by the linkage mechanism. This can prevent interference of the signal light by the residual light of the light source module 100 during detection.
When the detector 400 collects the light signal derived from the light outlet 320 after the excitation light excites the object to be detected 220, the light source module 100 emits the residual light in a short time (for example, 200 ms) although the light source module 100 is turned off electronically,
after the light source module 100 is turned off, there is a residual light for a short time, and since the power of the light source module 100 is greater than that of the detector 400, the residual light of the light source module 100 may affect the detection value of the detector 400. In this embodiment, after the first shutter assembly 500 is opened, the light source module 100 and the sample storage portion 200 can be isolated, when the first shutter assembly 500 is closed, the second shutter assembly 800 is opened simultaneously, so that the detector 400 is ensured not to be interfered by the residual light of the excitation light when receiving the signal light, the detection precision of the signal light is improved, and the detector 400 is protected from being damaged.
The light-activated chemiluminescence detection device of the application is described above, and correspondingly, the application also provides a control method of the light-activated chemiluminescence detection device.
Fig. 3 is a flow chart of a photo-activated chemiluminescence detection method according to an embodiment of the disclosure.
Referring to fig. 1 and 3, the method provided in this embodiment includes the following steps:
s110, the sample storage section 200 with the sample is placed on the signal light collecting member 300.
In this step, the signal light collecting member 300 is made of an opaque material, the signal light collecting member 300 is coated with an opaque material, and the sample storage part 200 is made of an opaque material, for example, the sample storage part 200 may be a transparent cup body having high transmittance. The sample storage section 200 with the sample may be placed into the signal light collecting member 300 from the mounting port 310 of the signal light collecting member 300.
S120, controlling the light source module 100 to project excitation light to the sample storage part 200, so that the sample performs photochemical reaction under the excitation of the excitation light; the signal light collecting element 300 is formed with a cavity 330 inside, the sample storage portion 200 is at least partially accommodated in the cavity 330, the inner wall of the cavity 330 is a reflecting surface, the signal light collecting element 300 is provided with a light outlet 320 communicated with the cavity 330, and the reflecting surface is used for converging signal light generated by a sample to the light outlet 320.
In this step, the light source module 100 is controlled to emit excitation light to the sample storage unit 200 for a predetermined period of time, and then the light source module 100 is turned off.
S130, when the reaction is completed, the detector 400 receives and detects the signal light emitted from the sample in the sample storage section 200 through the light outlet 320.
In this step, a detection lens 410 for receiving the signal light is provided on a side of the detector 400 opposite to the light outlet 320, and the detector 400 can convert the received light signal into an electrical signal.
In the solution provided in this embodiment, after the light is optically processed by the signal light collecting element 300, the light can be collected at the light outlet 320, and a detection lens 410 for receiving the signal light is disposed on a side of the detector 400 opposite to the light outlet 320, where the detector 400 can convert the received light signal into an electrical signal, so that the analyzer can detect the concentration of the detected object 220 through the electrical signal.
Referring to fig. 4, another embodiment of the present application provides a method including:
in step S210, the sample storage section 200 with the sample is placed on the signal light collecting member 300.
This step is described with reference to step S110, and will not be described here.
Step S220, controlling the first shutter assembly 500 to open so that the excitation light emitted from the light source module 100 is projected into the sample storage portion 200 through the excitation light path; when the first shutter assembly 500 is opened for a preset period of time, the first shutter assembly 500 is controlled to be closed, so that the sample storage portion 200 is in a light-tight environment, and the sample is subjected to photochemical reaction under the excitation of the excitation light.
The signal light collecting piece is provided with a light outlet communicated with the cavity, and the reflecting surface is used for converging signal light generated by a sample to the light outlet.
In step S230, after the reaction is completed, the second shutter assembly 800 is opened at a predetermined timing, so that the detector 400 receives and detects the signal light emitted from the sample in the sample storage portion 200 through the light outlet 320.
In this embodiment, the first shutter assembly 500 and the second shutter assembly 800 of this embodiment may be any structure capable of implementing on-off of an optical path, and the first shutter assembly 500 and the second shutter assembly 800 may be shutters in related art, for example, shutters similar to a camera. After the first shutter assembly 500 is opened, the light source module 100 and the sample storage portion 200 can be isolated, when the first shutter assembly 500 is closed, the second shutter assembly 800 is opened at the same time, so that the detector 400 is prevented from being interfered by the residual excitation light when receiving the signal light, the detection precision of the signal light is improved, and the detector 400 is prevented from being damaged.
The embodiment of the application further provides a light-activated chemiluminescence analyzer, which includes the light-activated chemiluminescence detection device of the above embodiment, and the structure of the light-activated chemiluminescence detection device is described in the above embodiment, which is not repeated herein.
The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (8)
1. A photo-activated chemiluminescent detection device comprising:
a sample storage section for storing a sample to be detected;
a light source module, an excitation light path is arranged between the light source module and the sample storage part, and the light source module projects excitation light to the sample storage part through the excitation light path;
the signal light collecting part is arranged on the signal light collecting part, a cavity is formed in the signal light collecting part, the sample storing part is at least partially accommodated in the cavity, the inner wall of the cavity is a reflecting surface, the signal light collecting part is provided with a light outlet communicated with the cavity, and the reflecting surface is used for converging signal light generated by the sample to the light outlet; the signal light collecting piece is provided with a mounting port, the sample storage part is inserted into the signal light collecting piece from the mounting port, and the light outlet is arranged at one end of the signal light collecting piece far away from the mounting port;
an opening is formed in one side, facing the light source module, of the sample storage part, and a light-sealing structure is arranged at the opening;
the detector is arranged at the light outlet, a detection light path is arranged between the light outlet and the detector, and the detector is used for receiving the signal light led out from the light outlet through the detection light path; the light source module, the sample storage portion, the signal light collecting member, and the detector are arranged along a center line of the signal light collecting member; the first shutter assembly is arranged between the light source module and the sample storage part and is used for switching on or switching off the excitation light path; in each direction of the same horizontal height, the distance between the sample storage part and the signal light collecting part is the same, and in different directions, the signal light can be converged at the light outlet after being reflected by the reflecting surface for one or more times, and then is received by the detector;
the second shutter assembly is arranged between the light outlet and the detector and is used for switching on or switching off the detection light path;
a controller electrically connected to the first shutter assembly and the second shutter assembly for controlling the first shutter assembly to open and the second shutter assembly to close during a first period of time; or, when the first shutter assembly is controlled to be closed in a second period, the second shutter assembly is opened.
2. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the signal light collecting piece is of a spherical structure, the spherical surface inside the spherical structure is the reflecting surface, and the sample storage part is arranged at the center close to the inside of the spherical structure.
3. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the signal light collecting member is made of a light-impermeable material, and a portion of the sample storage portion located in the signal light collecting member is made of a light-permeable material; alternatively, the signal light collecting member is coated with an opaque material, and the portion of the sample storage portion located in the signal light collecting member is made of an opaque material.
4. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the first shutter assembly and the second shutter assembly are connected through a linkage mechanism, and when the first shutter assembly is opened, the linkage mechanism drives the second shutter assembly to be closed; when the first shutter assembly is closed, the second shutter assembly is driven to be opened through the linkage mechanism; or,
the first shutter assembly and the second shutter assembly are opened or closed according to a preset time sequence.
5. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the mounting port and the light outlet are respectively arranged at two ends of the signal light collecting piece on the central line.
6. A photo-activated chemiluminescent detector comprising a photo-activated chemiluminescent detection assembly of any one of claims 1-5.
7. A detection method of the photoexcitation chemiluminescent detector of claim 6, comprising:
placing a sample storage part with a sample on a signal light collecting piece;
controlling a light source module to project excitation light to the sample storage part, so that the sample is subjected to photochemical reaction under the excitation of the excitation light; the signal light collecting piece is provided with a light outlet communicated with the cavity, and the reflecting surface is used for converging signal light generated by the sample to the light outlet;
after the reaction is completed, the detector receives and detects the signal light emitted by the sample in the sample storage part through the light outlet.
8. The method of detecting according to claim 7, wherein:
the control light source module projects excitation light to the sample storage part to enable the sample to perform photochemical reaction under the excitation of the excitation light, and the control light source module comprises:
controlling the first shutter assembly to be opened so that excitation light emitted by the light source module is projected into the sample storage part through an excitation light path; when the first shutter assembly is opened for a preset time period, the first shutter assembly is controlled to be closed, so that the sample storage part is in a light-tight environment, and the sample is subjected to photochemical reaction under the excitation of excitation light; or alternatively, the first and second heat exchangers may be,
after the reaction is completed, the detector receives and detects the signal light emitted by the sample in the sample storage part through the light outlet, and the signal light comprises:
when the reaction is completed, the second shutter assembly is opened at a predetermined timing so that the detector receives and detects the signal light emitted from the sample in the sample storage section through the light outlet.
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| CN202310808476.7A CN116519673B (en) | 2023-07-04 | 2023-07-04 | Light excitation chemiluminescence detection device, method and light excitation chemiluminescence detector |
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Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02236453A (en) * | 1989-03-10 | 1990-09-19 | Jeol Ltd | Detection of quantity of emitted light of immunoassay device |
| US5082630A (en) * | 1990-04-30 | 1992-01-21 | The United States Of America As Represented By The United States Department Of Energy | Fiber optic detector for immuno-testing |
| KR200256599Y1 (en) * | 2001-08-30 | 2001-12-24 | 주식회사 신코 | High Resolution Multi Functional Spectrophotometer for Color Measurement |
| CN1900718A (en) * | 2006-07-27 | 2007-01-24 | 华南师范大学 | Chemical light emitting method and its device for high snesitivity detecting micro albumin |
| CN107490432A (en) * | 2017-04-27 | 2017-12-19 | 安徽华脉科技发展有限公司 | A kind of Feebleness Light Signal Examining system |
| CN107532993A (en) * | 2015-02-10 | 2018-01-02 | 圣安德鲁斯大学董事会 | Use the systems, devices and methods of integrating sphere light collector |
| CN209014458U (en) * | 2018-10-30 | 2019-06-21 | 蓝怡科技集团股份有限公司 | A kind of integrating sphere photodetector and optical measuring system |
| CN110118763A (en) * | 2019-05-31 | 2019-08-13 | 宁夏大学 | A kind of the luminescence generated by light test device and its method of Weak-luminescence material |
| CN110199180A (en) * | 2016-10-11 | 2019-09-03 | 维多利亚联合有限公司 | The spectrometer device of the spectrum of fluid sample is measured for using integral chamber |
| CN110638426A (en) * | 2019-07-22 | 2020-01-03 | 邱海林 | Laser scanning living body fluorescence imaging signal acquisition device |
| CN112362882A (en) * | 2018-02-11 | 2021-02-12 | 博阳生物科技（上海）有限公司 | Full-automatic light-activated chemiluminescence detector |
| CN213516977U (en) * | 2020-10-23 | 2021-06-22 | 苏州大学 | Multi-dimensional electrochemical detection device |
| CN113376127A (en) * | 2020-02-25 | 2021-09-10 | 上海交通大学 | Imaging system suitable for multiple detection of biomolecules |
| CN116297378A (en) * | 2023-05-24 | 2023-06-23 | 科美诊断技术股份有限公司 | Light detection measuring system |
| CN116297416A (en) * | 2023-05-22 | 2023-06-23 | 科美诊断技术股份有限公司 | Photo-excitation chemiluminescence detection device and detection method |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6975891B2 (en) * | 2001-12-21 | 2005-12-13 | Nir Diagnostics Inc. | Raman spectroscopic system with integrating cavity |
| JP5148387B2 (en) * | 2008-06-30 | 2013-02-20 | 浜松ホトニクス株式会社 | Spectrometer, spectroscopic method, and spectroscopic program |
| WO2014152845A1 (en) * | 2013-03-20 | 2014-09-25 | Siemens Healthcare Diagnostics Inc. | Light and shutter for a sample analyzer |
| US9423346B2 (en) * | 2014-03-14 | 2016-08-23 | Datacolor Holding Ag | System and method for haze measurement |
-
2023
- 2023-07-04 CN CN202310808476.7A patent/CN116519673B/en active Active
Patent Citations (15)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPH02236453A (en) * | 1989-03-10 | 1990-09-19 | Jeol Ltd | Detection of quantity of emitted light of immunoassay device |
| US5082630A (en) * | 1990-04-30 | 1992-01-21 | The United States Of America As Represented By The United States Department Of Energy | Fiber optic detector for immuno-testing |
| KR200256599Y1 (en) * | 2001-08-30 | 2001-12-24 | 주식회사 신코 | High Resolution Multi Functional Spectrophotometer for Color Measurement |
| CN1900718A (en) * | 2006-07-27 | 2007-01-24 | 华南师范大学 | Chemical light emitting method and its device for high snesitivity detecting micro albumin |
| CN107532993A (en) * | 2015-02-10 | 2018-01-02 | 圣安德鲁斯大学董事会 | Use the systems, devices and methods of integrating sphere light collector |
| CN110199180A (en) * | 2016-10-11 | 2019-09-03 | 维多利亚联合有限公司 | The spectrometer device of the spectrum of fluid sample is measured for using integral chamber |
| CN107490432A (en) * | 2017-04-27 | 2017-12-19 | 安徽华脉科技发展有限公司 | A kind of Feebleness Light Signal Examining system |
| CN112362882A (en) * | 2018-02-11 | 2021-02-12 | 博阳生物科技（上海）有限公司 | Full-automatic light-activated chemiluminescence detector |
| CN209014458U (en) * | 2018-10-30 | 2019-06-21 | 蓝怡科技集团股份有限公司 | A kind of integrating sphere photodetector and optical measuring system |
| CN110118763A (en) * | 2019-05-31 | 2019-08-13 | 宁夏大学 | A kind of the luminescence generated by light test device and its method of Weak-luminescence material |
| CN110638426A (en) * | 2019-07-22 | 2020-01-03 | 邱海林 | Laser scanning living body fluorescence imaging signal acquisition device |
| CN113376127A (en) * | 2020-02-25 | 2021-09-10 | 上海交通大学 | Imaging system suitable for multiple detection of biomolecules |
| CN213516977U (en) * | 2020-10-23 | 2021-06-22 | 苏州大学 | Multi-dimensional electrochemical detection device |
| CN116297416A (en) * | 2023-05-22 | 2023-06-23 | 科美诊断技术股份有限公司 | Photo-excitation chemiluminescence detection device and detection method |
| CN116297378A (en) * | 2023-05-24 | 2023-06-23 | 科美诊断技术股份有限公司 | Light detection measuring system |
Non-Patent Citations (1)
| Title |
|---|
| 中国金属学会，中国有色金属学会编.《金属材料物理性能手册 第1册 金属物理性能及测试方法》.冶金工业出版社,1987,第410-411页. * |
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