CN116539599A - Photo-excitation chemiluminescence detection device, control method and photo-excitation chemiluminescence analyzer - Google Patents
Photo-excitation chemiluminescence detection device, control method and photo-excitation chemiluminescence analyzer Download PDFInfo
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- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000002038 chemiluminescence detection Methods 0.000 title claims abstract description 19
- 230000001443 photoexcitation Effects 0.000 title claims abstract description 15
- 230000005284 excitation Effects 0.000 claims abstract description 130
- 230000007246 mechanism Effects 0.000 claims abstract description 52
- 238000001514 detection method Methods 0.000 claims abstract description 45
- 230000003287 optical effect Effects 0.000 claims abstract description 13
- 230000005540 biological transmission Effects 0.000 claims description 4
- 238000003018 immunoassay Methods 0.000 description 6
- 230000008569 process Effects 0.000 description 6
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000003111 delayed effect Effects 0.000 description 3
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 238000003556 assay Methods 0.000 description 2
- 238000004020 luminiscence type Methods 0.000 description 2
- 239000000126 substance Substances 0.000 description 2
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 239000000427 antigen Substances 0.000 description 1
- 102000036639 antigens Human genes 0.000 description 1
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- 229940079593 drug Drugs 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 229930195729 fatty acid Natural products 0.000 description 1
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- 229940088597 hormone Drugs 0.000 description 1
- 230000008105 immune reaction Effects 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000012535 impurity Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000003127 radioimmunoassay Methods 0.000 description 1
- 230000035945 sensitivity Effects 0.000 description 1
- 229910052710 silicon Inorganic materials 0.000 description 1
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
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- G01N21/75—Systems in which material is subjected to a chemical reaction, the progress or the result of the reaction being investigated
- G01N21/76—Chemiluminescence; Bioluminescence
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- G—PHYSICS
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- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/01—Arrangements or apparatus for facilitating the optical investigation
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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- Y02B—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO BUILDINGS, e.g. HOUSING, HOUSE APPLIANCES OR RELATED END-USER APPLICATIONS
- Y02B20/00—Energy efficient lighting technologies, e.g. halogen lamps or gas discharge lamps
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Abstract
The application relates to a light excitation chemiluminescence detection device, a control method and a light excitation chemiluminescence analyzer. The photo-excitation chemiluminescence detection device comprises a sample storage part, wherein the sample storage part is used for storing a detected sample; at least two light source modules which are arranged at the side of the sample storage part and respectively project excitation light to the sample of the sample storage part through excitation light paths; a detector for receiving an optical signal generated after the sample is excited by the excitation light through a receiving optical path; and the shutter mechanism is used for controlling the on-off of the excitation light path and the receiving light path according to a preset time sequence. According to the scheme provided by the application, the multiple excitation and multiple detection of the sample can be realized.
Description
Technical Field
The present disclosure relates to the field of biochemical detection, and in particular, to a light-activated chemiluminescence detection device, a control method, and a light-activated chemiluminescence analyzer.
Background
Chemiluminescent immunoassay (chemiluminescence immunoassay, CLIA) is a detection assay technique for various antigens, haptens, antibodies, hormones, enzymes, fatty acids, vitamins, drugs, etc., by combining a chemiluminescent assay technique with a high sensitivity with a highly specific immune reaction. Is a latest immunoassay technology developed after the analysis of radioimmunoassay, enzyme immunoassay, fluorescence immunoassay and time-resolved fluorescence immunoassay.
In the related art, detection of the components and contents of substances contained in a sample is achieved by detecting the luminescence intensity of a reaction solution of the sample to be detected. Generally, the optical signal of the detected object is detected after one-time excitation, and as the photo-excitation chemiluminescence instrument is more and more complex, the reagent reaction requirement is higher and the reaction of some reagents cannot be satisfied for one-time excitation and one-time detection.
Disclosure of Invention
In order to solve or partially solve the problems existing in the related art, the application provides a light excitation chemiluminescence detection device, a control method and a light excitation chemiluminescence analyzer, which can realize multiple excitation and multiple detection of a sample.
A first aspect of the present application provides a photoexcitation chemiluminescent detection device, comprising:
a sample storage section for storing a sample to be detected;
at least two light source modules arranged at the side of the sample storage part and used for respectively projecting excitation light to the sample of the sample storage part through an excitation light path;
a detector for receiving an optical signal generated after the sample is excited by the excitation light through a receiving optical path;
and the shutter mechanism is used for controlling the on-off of the excitation light path and the receiving light path according to a preset time sequence.
In some embodiments, the shutter mechanism includes a first accommodating member, the first accommodating member may rotate around an axis thereof, the at least two light source modules and the detector are disposed at equal angular intervals on a side of the first accommodating member, a darkroom is formed in the first accommodating member, the sample storage portion is disposed in the darkroom, and a through hole is formed in a side wall of the first accommodating member;
when the first accommodating part rotates according to a preset time sequence, the excitation light path is conducted through the through hole; or, the receiving light path is conducted through the through hole.
In some embodiments, the first accommodating part is used for being conducted with the excitation light path or the receiving light path one by one according to a preset time sequence; the driving device is in transmission connection with the first accommodating piece and is used for driving the first accommodating piece to rotate.
In some embodiments, the at least two light source modules have different powers or are capable of emitting the excitation light of different wavelengths for multiple excitation of the sample with the excitation light of multiple different wavelengths or multiple different powers.
In some embodiments, the shutter mechanism includes a second accommodating member fixedly disposed with respect to the light source module and the detector, a darkroom is formed in the second accommodating member, and the sample storage portion is disposed in the darkroom; the shutter mechanism comprises a plurality of shutters arranged on the side wall of the second accommodating part, and when the shutters are opened and closed at a preset time sequence, the excitation light path is conducted, and the receiving light path is cut off; or, the excitation light path is cut off, and the receiving light path is conducted.
In some embodiments, the plurality of shutters are arranged in a linkage, when one of the shutters is opened, the rest of the shutters are closed by linkage;
the controller is electrically connected with the shutter mechanism and the at least two light source modules and is used for controlling the shutter mechanism to operate and controlling the at least two light source modules to be opened or closed.
A second aspect of the present application provides a control method of a light-activated chemiluminescent detection device, where the light-activated chemiluminescent detection device includes a sample storage portion, at least two light source modules, a detector, and a shutter mechanism, where the shutter mechanism includes a first housing member rotatable about an axis thereof, and at least two of the light source modules and the detector are equiangularly spaced apart from each other on a side surface of the first housing member; a darkroom for accommodating a sample is formed in the first accommodating part, and a through hole is formed in the side wall of the first accommodating part;
the control method comprises the following steps:
controlling the first accommodating part of the shutter mechanism to rotate to a first state so as to conduct an excitation light path between one of the at least two light source modules and the sample storage part through the through hole;
the shutter mechanism is controlled to operate to a second state to conduct a receiving light path between the at least two light source modules and the detector through the through hole.
In some embodiments, after controlling the shutter mechanism to operate to the first state, comprising:
delaying a first preset time period, controlling the first accommodating part to rotate to a preset position for enabling the sample storage part to be densely illuminated, and controlling the first accommodating part to rotate to the second state after delaying a second time period; and/or the number of the groups of groups,
after controlling the first accommodating part to rotate to the second state, the method comprises the following steps:
and controlling the first accommodating part to rotate to the first state after delaying the second preset time period.
A third aspect of the present application provides a photo-activated chemiluminescent analyzer comprising a photo-activated chemiluminescent detection device as described in the first aspect above.
In some embodiments, the shutter mechanism includes a second accommodating member fixedly disposed with respect to the light source module and the detector, a darkroom is formed in the second accommodating member, and the sample storage portion is disposed in the darkroom; the shutter mechanism comprises a plurality of shutters mounted on the side wall of the second accommodating piece;
the control method comprises the following steps:
controlling one of a plurality of shutters corresponding to the light source modules to open so as to conduct an excitation light path between one of at least two light source modules and the sample storage portion, and simultaneously cut off a receiving light path between the sample storage portion and the detector;
and controlling the shutter corresponding to the detection modules to open so as to cut off the excitation light path between at least two light source modules and the sample storage part and simultaneously conduct the receiving light path between the sample storage part and the detector.
The technical scheme that this application provided can include following beneficial effect:
the shutter mechanism can be switched between a first state and a second state according to a preset time sequence, so that after one light source module is excited and detected by the detector, the excitation of the next laser module and the detection process of the detector are executed, the excitation light paths and the receiving light paths corresponding to the light source modules are conducted or cut off one by one, and the light paths are reciprocated to realize multiple excitation and multiple detection of a sample; when the shutter mechanism operates, isolation can be formed between at least two light source modules and between the light source modules and the detector, so that the detector is prevented from being interfered by residual excitation light when receiving signal light, the detection precision of the signal light is improved, and the detector is prevented from being damaged.
It is to be understood that both the foregoing general description and the following detailed description are exemplary and explanatory only and are not restrictive of the application.
Drawings
The foregoing and other objects, features and advantages of the application will be apparent from the following more particular descriptions of exemplary embodiments of the application as illustrated in the accompanying drawings wherein like reference numbers generally represent like parts throughout the exemplary embodiments of the application.
FIG. 1 is a top view of a photo-activated chemiluminescent detection device according to one embodiment of the present application;
FIG. 2 is a side view of the photo-activated chemiluminescent detection assembly shown in the embodiment of FIG. 1;
FIG. 3 is a top view of a photo-activated chemiluminescent detection device according to an embodiment of the present application;
FIG. 4 is a flow chart of a control method according to an embodiment of the present disclosure;
fig. 5 is a flow chart of a control method according to another embodiment of the present application.
Reference numerals: 100. a light source module; 200. a detector; 210. a light filter; 300. a first receiving member; 310. a darkroom; 311. a sample storage unit; 320. a through hole; 410. a first lens group; 420. a second lens group; a. An excitation light path; B. a receiving optical path;
Detailed Description
Embodiments of the present application will be described in more detail below with reference to the accompanying drawings. While embodiments of the present application are shown in the drawings, it should be understood that the present application may be embodied in various forms and should not be limited to the embodiments set forth herein. Rather, these embodiments are provided so that this disclosure will be thorough and complete, and will fully convey the scope of the disclosure to those skilled in the art.
The terminology used in the present application is for the purpose of describing particular embodiments only and is not intended to be limiting of the present application. As used in this application and the appended claims, the singular forms "a," "an," and "the" are intended to include the plural forms as well, unless the context clearly indicates otherwise. It should also be understood that the term "and/or" as used herein refers to and encompasses any or all possible combinations of one or more of the associated listed items.
It should be understood that although the terms "first," "second," "third," etc. may be used herein to describe various information, these information should not be limited by these terms. These terms are only used to distinguish one type of information from another. For example, a first message may also be referred to as a second message, and similarly, a second message may also be referred to as a first message, without departing from the scope of the present application. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include one or more such feature. In the description of the present application, the meaning of "a plurality" is two or more, unless explicitly defined otherwise.
In the related art, detection of the components and contents of substances contained in a sample is achieved by detecting the luminescence intensity of a reaction solution of the sample to be detected. Generally, the optical signal of the detected object is detected after one-time excitation, and as the photo-excitation chemiluminescence instrument is more and more complex, the reagent reaction requirement is higher and the reaction of some reagents cannot be satisfied for one-time excitation and one-time detection.
In view of the above problems, embodiments of the present application provide a light excitation chemiluminescence detection device, which can perform multiple excitation and multiple detection on a sample.
The following describes the technical scheme of the embodiments of the present application in detail with reference to the accompanying drawings.
FIG. 1 is a side view of a photo-activated chemiluminescent detection device according to an embodiment of the present application; fig. 2 is a top view of the photo-activated chemiluminescent detection device of the embodiment of fig. 1.
Referring to fig. 1 and 2, the photo-excitation chemiluminescence detection device provided by the present application includes a sample storage portion 311, where the sample storage portion 311 is configured to store a detected sample, and the sample may be a reaction solution; at least two light source modules 100 disposed around the sample storage portion 311 and configured to project excitation light to the sample of the sample storage portion 311 through the excitation light path a, respectively; a detector 200 for receiving an optical signal generated after each excitation of the sample through a receiving optical path B; and the shutter mechanism is used for controlling the on-off of the excitation light path A and the receiving light path B according to a preset time sequence.
The shutter operation process has a first state in which one of the at least two light source modules 100 is on with the excitation light path a between the sample storage portion 311 while the reception light path B between the sample storage portion 311 and the detector 200 is off; in the second state, the excitation light path a between at least two light source modules 100 and the sample storage portion 311 is cut off, and the reception light path B between the sample storage portion 311 and the detector 200 is turned on. In this embodiment, the shutter mechanism can switch between the first state and the second state according to a predetermined time sequence, and after one light source module 100 is excited and detected by the detector 200, the excitation of the next light source module 100 and the detection process of the detector 200 are performed, so that the shutter mechanism reciprocates, and multiple excitation and multiple detection of the sample can be realized.
In this embodiment, at least two light source modules 100 and detectors 200 are disposed at the sides of the sample storage portion 311, the light emitting sides of the light source modules 100 face the sample storage portion 311, the detection surfaces of the detectors 200 face the sample storage portion 311, and the sample storage portion 311 may be a transparent container containing a sample, such as a test tube or a cuvette.
In some embodiments, the light source module 100 may be a laser emitter, and the detector 200 may be one of a single photon counter, a silicon photocell, an integrating sphere, and a photomultiplier tube.
The photo-activated chemiluminescence detection device of the embodiment further comprises a controller, wherein the controller is electrically connected with the shutter mechanism and used for controlling the shutter mechanism to operate at a preset time sequence. The preset timing may be set in accordance with the number of the light source modules 100 so that when the shutter mechanism is operated, the excitation light path a between each light source module 100 and the sample storage portion 311 is turned on or off at different periods, or the reception light path between the sample storage portion 311 and the detector 200 is turned on or off at different periods.
In some embodiments, the controller is further electrically connected to the at least two light source modules 100, and is configured to control the at least two light source modules 100 to be turned on or off at a preset time sequence, wherein after the light source modules 100 are turned off, the excitation light path a is simultaneously cut off by the shutter mechanism.
After the light source module 100 is turned off, there is a residual light for a short time, and since the power of the light source module 100 is greater than that of the detector 200, the residual light of the light source module 100 may affect the detection value of the detector 200. In this embodiment, when the shutter mechanism operates, at least two light source modules 100 and at least two light source modules 200 can be isolated, so that when the detector 200 receives signal light, the signal light is prevented from being interfered by residual excitation light, the detection precision of the signal light is improved, and the detector 200 is prevented from being damaged.
In some embodiments, a first lens group 410 is disposed between the light source module 100 and the sample storage portion 311, and the first lens group 410 is used to filter or collimate the excitation light emitted by the light source module 100 and then transmit the filtered or collimated excitation light to the sample storage portion 311 along the excitation light path a.
In some embodiments, a second lens group 420 is disposed between the sample storage 311 and the detector 200, and the second lens group 420 can focus the signal light emitted from the sample into a light spot and then project the light spot to the receiving lens of the detector 200.
In some embodiments, a filter 210 is disposed on a receiving lens side of the detector 200, and the filter 210 is used for filtering signal light, so that wavelengths truly reflected by a sample can be collected on the receiving lens of the detector 200 in a pure manner, and unwanted light with interference wavelengths and impurity wavelengths can be filtered.
In some embodiments, the wavelength range of the excitation light emitted by the light source module 100 may be 675nm-685nm. At least two light source modules 100 have the same power or are capable of emitting excitation light of the same power.
In some embodiments, at least two light source modules 100 have different powers or are capable of emitting excitation light of different wavelengths. By arranging at least two light source modules 100 so as to be capable of emitting excitation light of different wavelengths, multiple excitation of excitation light of multiple different wavelengths or multiple different powers can be achieved.
With continued reference to fig. 1 and 2, in some embodiments, the shutter mechanism includes a first housing member 300, where the first housing member 300 is rotatable relative to the light source module 100 and the detector 200, for example, in fig. 2, the first housing member rotates about an axis L thereof, and at least two light source modules 100 and the detector 200 are equiangularly spaced apart on a side of the first housing member 300, for example, at different rotational angles of the first housing member 300. A darkroom 310 is formed in the first accommodating piece 300, the sample storage part 311 is fixed in the darkroom 310, the first accommodating piece 300 can rotate relative to the sample storage part 311, and a through hole 320 is formed in the side wall of the first accommodating piece 300; when the first receiving member 300 rotates according to a preset time sequence, the excitation light path a is conducted through the through hole 320, and the receiving light path B is cut off by the side wall of the first receiving member 300; alternatively, the excitation light path a is cut off by the side wall of the first housing 300, and the reception light path B is conducted through the through hole 320.
Referring to fig. 1, in some embodiments, the first receiving member 300 is electrically connected to the excitation light path a or the receiving light path B one by one at a predetermined timing, where the predetermined timing includes a first state and a second state. In the first state, the first accommodating member 300 rotates to a first angle, and the through hole 320 is opposite to one of the light source modules 100, so that the excitation light path a is conducted, and the receiving light path B is cut off by the side wall of the first accommodating member 300; in the second state, the first receiving member 300 is rotated to a second angle, and the through hole 320 is opposite to the detector 200, so that the excitation light path a is cut off by the sidewall of the first receiving member 300, and the receiving light path B is conducted by the through hole 320.
One through hole 320 is formed in the first accommodating member 300, and when the first accommodating member 300 rotates, one through hole 320 conducts one of the excitation light paths a or the receiving light paths B corresponding to at least two light source modules 100 one by one.
In some embodiments, the light-activated chemiluminescence detection device further includes a driving device, where the driving device is in transmission connection with the first accommodating element 300 and is used for driving the first accommodating element 300 to rotate. The driving device is electrically connected with a controller, and the controller is used for controlling the driving device to operate. The driving device may be a servo motor, and an output shaft of the motor is in transmission connection with the first accommodating member 300 and can drive the first accommodating member 300 to rotate.
Referring to fig. 3, in some embodiments, three light source modules, namely, a first light source module 100a, a second light source module 100b and a third light source module 100c, are provided, and the first light source module 100a, the second light source module 100b, the third light source module 100c and the detector 200 are arranged in a ring array and around the first accommodating member 300, and a through hole 320 is formed on a sidewall of the first accommodating member 300.
The sample of this embodiment may be divided into three excitations, three detections, and the processes of each excitation and detection are as follows:
the first container 300 is controlled to rotate, the through hole 320 of the first container 300 is stopped after rotating to the position corresponding to the first light source module 100a, the excitation light path a between the first light source module 100a and the sample storage part 311 is conducted, and the light beam emitted by the first light source module 100a can irradiate the sample in the first container 300 through the excitation light path A1, so that excitation of the sample by the excitation light of the first light source module 100 is realized. After the set period of time (for example, 300 ms) is excited, the first accommodating member 300 is controlled to rotate again, the first accommodating member 300 rotates until the through hole 320 of the first accommodating member 300 is staggered with the first light source module 100a, the excitation light path A1 is cut off by the side wall of the first accommodating member 300, the cut-off state can be delayed for the set period of time (for example, 200 ms), then the first accommodating member 300 is controlled to rotate until the through hole 320 of the first accommodating member corresponds to the detector 200 and then stops, at this time, the receiving light path B is conducted by the through hole 320, and the detector 200 receives the signal light emitted by the sample through the receiving light path B.
After the excitation and detection of the first light source module 100a are completed, the first accommodating member 300 is controlled to continue to rotate, so that the through hole 320 of the first accommodating member 300 rotates to correspond to the second light source module 100b and then stops, an excitation light path A2 between the second light source module 100b and the sample storage part 311 is conducted, and a light beam emitted by the second light source module 100b can irradiate the sample of the sample storage part 311 in the first accommodating member 300 through the excitation light path A2, so that excitation of the sample by the excitation light of the second light source module 100b is realized. After the excitation is completed, the first accommodating part 300 is controlled to rotate again, the first accommodating part 300 rotates until the through hole of the first accommodating part 300 is staggered with the second light source module 100B, the excitation light path A2 is cut off, the first accommodating part 300 is controlled to rotate until the through hole of the first accommodating part corresponds to the detector 200, then the first accommodating part is stopped, the receiving light path B is conducted by the through hole, and the detector 200 receives signal light emitted by a sample through the receiving light path B.
Referring to fig. 3, after the second light source module 100b is excited and detected, the first accommodating member 300 is controlled to rotate again, so that the through hole of the first accommodating member 300 rotates to correspond to the third light source module 100c and then stops, the excitation light path A3 between the third light source module 100c and the sample storage portion 311 is turned on, and the light beam emitted by the third light source module 100c can irradiate the sample in the first accommodating member 300 through the excitation light path A3, so that excitation of the sample by the excitation light of the third light source module 100c is realized. After the excitation is completed, the first accommodating part 300 is controlled to rotate again, the first accommodating part 300 rotates until the through hole 320 of the first accommodating part is staggered with the third light source module 100c, the excitation light path A3 is cut off, the first accommodating part 300 is controlled to rotate until the through hole of the first accommodating part corresponds to the detector 200, the receiving light path B is conducted by the through hole, and the detector 200 receives signal light emitted by a sample through the receiving light path B.
Referring to fig. 3, in the present embodiment, the first light source module 100a, the second light source module 100b and the third light source module 100c are three different light source modules, and the differences include, but are not limited to, different wavelengths or different powers of excitation light, so that multiple excitation of different excitation wavelengths of the object to be detected can be realized, and multiple detection of the sample can be realized.
By the above manner, the shutter mechanism realizes that the excitation light path a and the reception light path B are conducted one by one between the plurality of light source modules 100.
With continued reference to fig. 1 and 2, in the present embodiment, since only one through hole 320 is formed in the first accommodating member 300, when the through hole 320 rotates to be opposite to the detector 200, the excitation light path a is simultaneously cut off by the side wall of the first accommodating member 300, so that interference of residual light of the light source module 100 can be avoided. The shutter mechanism of this embodiment has a simpler structure, and the first receiving member 300 is controlled to rotate at a set time sequence to realize the connection or disconnection of the excitation light path a and the receiving light path B one by one in different time periods.
In this embodiment, the first accommodating member 300 is cylindrical, a rotating shaft is disposed along the axis of the first accommodating member 300, the rotating shaft is connected to the output end of the driving device, the first accommodating member 300 is fixed on the rotating shaft, and when the rotating shaft rotates, the first accommodating member 300 rotates, so that the through holes can conduct the excitation light path a or the receiving light path B one by one according to a predetermined time sequence.
In this embodiment, a darkroom is formed in the first receiving member 300, and the sample storage portion is disposed in the darkroom of the first receiving member 300, and the first receiving member 300 is made of a light-impermeable material. The top of the first accommodating member 300 is provided with an opening, a cover body is mounted on the opening, after the cover body is opened, the sample storage portion 311 can be placed in the darkroom of the first accommodating member 300, and after the placement is completed, the cover body is covered again, so that a light-proof environment is formed in the darkroom of the first accommodating member 300.
In some embodiments, the shutter mechanism includes a second accommodating member (not shown) fixedly disposed with respect to the light source module 100 and the detector 200, a dark room is formed in the second accommodating member, and the sample storage portion 311 is disposed in the dark room; the shutter mechanism comprises a plurality of shutters arranged on the side wall of the second accommodating part, and when the shutters are opened and closed at a preset time sequence, the excitation light path A is conducted, and the receiving light path B is cut off; or, the excitation light path A is cut off, and the receiving light path B is conducted.
The shutter may be a shutter of a related art, for example, a shutter of a camera, and the controller is electrically connected to the plurality of shutters, and is capable of controlling the plurality of shutters to open or close at a predetermined timing, so as to switch on or switch off the excitation light path a and the receiving light path B in different time periods, so as to achieve multiple excitation of the sample by the excitation light emitted by the plurality of light source modules 100, and receive the signal light generated by the sample by the detector 200 after each excitation.
When the sample is excited, the shutter corresponding to the first light source module 100 is controlled to open, so that an excitation light path A between the first light source module 100 and the sample storage part 311 is conducted, and a light beam emitted by the first light source module 100 can irradiate the sample in the second accommodating part through the excitation light path A, so that excitation of the sample for a set period of time, for example, 300ms can be excited by the excitation light of the first light source module 100. After the excitation is completed, the shutter is controlled to close again, at this time, the excitation light path a is cut off, and the shutter corresponding to the detector 200 is controlled to open after a set period of time (for example, 200 ms) is delayed, so that the receiving light path B is conducted through the through hole 320, and the detector 200 receives the signal light emitted by the sample through the receiving light path B. After the excitation and detection of the first light source module 100 are completed, the shutter corresponding to the detector 200 is controlled to be closed, and the shutter corresponding to the second light source module 100 is controlled to be opened for the second excitation, and the excitation modes of the second light source module 100 and the third light source module 100 are referred to the above description of the first light source module 100, which is not repeated here.
In some embodiments, a plurality of shutters are arranged in a linkage manner, when one of the shutters is opened, the rest of the shutters are closed by linkage, and a controller is electrically connected with the shutter mechanism and the at least two light source modules 100, so as to control the shutter mechanism to operate and control the at least two light source modules 100 to be opened or closed. This can prevent interference of the residual light of the light source module 100 with the signal light during detection, and protect the detector 200 from damage.
The light-activated chemiluminescence detection device of the application is described above, and correspondingly, the application also provides a control method of the light-activated chemiluminescence detection device. The photo-activated chemiluminescence detection device comprises a sample storage portion 311, at least two light source modules 100, a detector 200 and a shutter mechanism, wherein the at least two light source modules 100 and the detector 200 are arranged at equal angular intervals on the side surface of a first accommodating member, a darkroom for accommodating a sample is formed in the first accommodating member 300, and a through hole 320 is formed in the side wall of the first accommodating member 300. The light source modules of the present embodiment are respectively used for projecting excitation light to the samples of the sample storage portion 311 through the excitation light path a, and the detector 200 is used for receiving the light signal generated after each excitation of the samples through the receiving light path B.
Fig. 4 is a flow chart of a control method according to an embodiment of the present application.
Referring to fig. 1, 2 and 4, the method provided in this embodiment includes the following steps:
s100, the first accommodating piece of the shutter mechanism is controlled to rotate to a first state so as to conduct an excitation light path A between one of the at least two light source modules 100 and the sample storage part 311 through the through hole.
In this step, after the excitation light path a is turned on, the reception light path B between the sample storage 311 and the detector 200 is cut off, and the excitation light energy emitted from the light source module 100 is projected to the sample storage 311, thereby exciting the sample.
S200, controlling the shutter mechanism to operate to the second state to conduct the receiving light path B between the at least two light source modules 100 and the detector 200 through the through-hole.
In this step, after the receiving optical path B is turned on, the detector 200 can receive the signal light generated after the sample is excited through the receiving optical path B.
According to the scheme provided by the embodiment, in the process of exciting the sample by each light source module 100, the shutter mechanism can be operated to the first state and the second state, so that the excitation of the sample and the detection of the signal light are realized in each excitation process, and the multiple excitation and the multiple detection of the sample can be realized.
In some embodiments, after the shutter mechanism is operated to the first state, the shutter mechanism includes: delaying a first preset time period, controlling the first accommodating part to rotate to a preset position for enabling the sample storage part to be densely illuminated, and controlling the first accommodating part to rotate to a second state after delaying a second time period. The first preset time period may be, for example, 200ms.
In some embodiments, after controlling the first accommodating member to rotate to the second state, the method includes: the first container is controlled to rotate to the first state after a second preset time period is delayed, and the second preset time period can be 600ms, for example. In the embodiment, the first accommodating piece is controlled to sequentially run to the first state, delay time and the shutter to run to the second state to reciprocate, so that multiple excitation and multiple detection of the sample are realized.
In some embodiments, when the shutter mechanism includes the first receiving member 300, the first receiving member 300 is rotatable relative to the light source module 100. The first receiving member 300 is first controlled to rotate to a first angle, so that the through hole 320 is opposite to one of the light source modules 100. The first receiving member 300 is then controlled to rotate to a second angle such that the through hole 320 is opposite to the detector 200. In this embodiment, the excitation light path a and the receiving light path B can be cut off and turned on at different periods by controlling the rotation setting angle of the first accommodating member 300, so that the structure of the shutter mechanism is simplified, and the control mode is simpler.
Referring to fig. 5, in some embodiments, the control method further includes:
and S300, controlling one of a plurality of shutters corresponding to the light source modules to be opened so as to conduct an excitation light path between one of the at least two light source modules and the sample storage part and cut off a receiving light path between the sample storage part and the detector.
S400, controlling the shutter corresponding to the detection module to open so as to cut off the excitation light path between at least two light source modules and the sample storage part and simultaneously conduct the receiving light path between the sample storage part and the detector.
The embodiment of the application further provides a light-activated chemiluminescence analyzer, which includes the light-activated chemiluminescence detection device of the above embodiment, and the structure of the light-activated chemiluminescence detection device is described in the above embodiment, which is not repeated herein.
The application also provides an electronic device comprising: a processor; and a memory having executable code stored thereon that, when executed by the processor, causes the processor to perform the method as described in the above embodiments.
The embodiments of the present application have been described above, the foregoing description is exemplary, not exhaustive, and not limited to the embodiments disclosed. Many modifications and variations will be apparent to those of ordinary skill in the art without departing from the scope and spirit of the various embodiments described. The terminology used herein was chosen in order to best explain the principles of the embodiments, the practical application, or the improvement of technology in the marketplace, or to enable others of ordinary skill in the art to understand the embodiments disclosed herein.
Claims (10)
1. A photo-activated chemiluminescent detection device comprising:
a sample storage section for storing a sample to be detected;
at least two light source modules arranged at the side of the sample storage part and used for respectively projecting excitation light to the sample of the sample storage part through an excitation light path;
a detector for receiving an optical signal generated after the sample is excited by the excitation light through a receiving optical path;
and the shutter mechanism is used for controlling the on-off of the excitation light path and the receiving light path according to a preset time sequence.
2. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the shutter mechanism comprises a first accommodating part, wherein the first accommodating part can rotate around the axis of the first accommodating part, at least two light source modules and the detector are arranged on the side face of the first accommodating part at equal angular intervals, a darkroom is formed in the first accommodating part, the sample storage part is arranged in the darkroom, and a through hole is formed in the side wall of the first accommodating part;
when the first accommodating part rotates according to a preset time sequence, the excitation light path is conducted through the through hole; or, the receiving light path is conducted through the through hole.
3. The photoexcitation chemiluminescent detection system of claim 2, wherein:
the first accommodating part is used for being conducted with the excitation light path or the receiving light path one by one according to a preset time sequence; the driving device is in transmission connection with the first accommodating piece and is used for driving the first accommodating piece to rotate.
4. The photoexcitation chemiluminescent detection system of claim 1, wherein:
the shutter mechanism comprises a second accommodating piece, the second accommodating piece is fixedly arranged relative to the light source module and the detector, a darkroom is formed in the second accommodating piece, and the sample storage part is arranged in the darkroom;
the shutter mechanism comprises a plurality of shutters arranged on the side wall of the second accommodating part, and when the shutters are opened and closed at a preset time sequence, the excitation light path is conducted, and the receiving light path is cut off; or, the excitation light path is cut off, and the receiving light path is conducted.
5. The photoexcitation chemiluminescent detection system of claim 4, wherein:
the plurality of shutters are arranged in a linkage way, and when one shutter is opened, the rest shutters are closed through linkage;
the controller is electrically connected with the shutter mechanism and the at least two light source modules and is used for controlling the shutter mechanism to operate and controlling the at least two light source modules to be opened or closed.
6. The photoexcitation chemiluminescent detection system according to any one of claims 1-5, wherein:
the at least two light source modules have different powers or are capable of emitting the excitation light of different wavelengths for multiple excitation of the sample with the excitation light of a plurality of different wavelengths or a plurality of different powers.
7. A control method of a light-activated chemiluminescence detection device is characterized by comprising the following steps:
the photo-excitation chemiluminescence detection device comprises a sample storage part, at least two light source modules, a detector and a shutter mechanism, wherein the shutter mechanism comprises a first accommodating piece, the first accommodating piece can rotate around the axis of the first accommodating piece, and the at least two light source modules and the detector are equiangular intervals arranged on the side face of the first accommodating piece; a darkroom for accommodating a sample is formed in the first accommodating part, and a through hole is formed in the side wall of the first accommodating part;
the control method comprises the following steps:
controlling the first accommodating part of the shutter mechanism to rotate to a first state so as to conduct an excitation light path between one of the at least two light source modules and the sample storage part through the through hole;
and controlling the first accommodating piece to rotate to a second state so as to conduct the receiving light paths between the at least two light source modules and the detector through the through holes.
8. The control method according to claim 7, characterized in that:
after controlling the first housing member of the shutter mechanism to rotate to the first state, comprising:
delaying a first preset time period;
controlling the first accommodating part to rotate to a preset position for enabling the sample storage part to be densely illuminated, and controlling the first accommodating part to rotate to the second state after delaying for a second time period; and/or the number of the groups of groups,
after controlling the first accommodating part to rotate to the second state, the method comprises the following steps:
and controlling the first accommodating part to rotate to the first state after delaying the second preset time period.
9. A control method of a light-activated chemiluminescence detection device is characterized by comprising the following steps:
the light-activated chemiluminescence detection device comprises a shutter mechanism, wherein the shutter mechanism comprises a second accommodating piece, the second accommodating piece is fixedly arranged relative to the light source module and the detector, a darkroom is formed in the second accommodating piece, and a sample storage part is arranged in the darkroom; the shutter mechanism comprises a plurality of shutters mounted on the side wall of the second accommodating piece;
the control method comprises the following steps:
controlling one of a plurality of shutters corresponding to the light source modules to open so as to conduct an excitation light path between one of at least two light source modules and the sample storage portion, and simultaneously cut off a receiving light path between the sample storage portion and the detector;
and controlling the shutter corresponding to the detection modules to open so as to cut off the excitation light path between at least two light source modules and the sample storage part and simultaneously conduct the receiving light path between the sample storage part and the detector.
10. A photo-activated chemiluminescent analyzer comprising a photo-activated chemiluminescent detection device of any one of claims 1-6.
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