CN116218782A - 细胞选择和修饰细胞代谢的方法 - Google Patents

细胞选择和修饰细胞代谢的方法 Download PDF

Info

Publication number
CN116218782A
CN116218782A CN202310027006.7A CN202310027006A CN116218782A CN 116218782 A CN116218782 A CN 116218782A CN 202310027006 A CN202310027006 A CN 202310027006A CN 116218782 A CN116218782 A CN 116218782A
Authority
CN
China
Prior art keywords
cell
cells
nucleic acid
lmm
sequence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202310027006.7A
Other languages
English (en)
Chinese (zh)
Inventor
R·杨
C·M·司迈尔斯
C·M·雅克斯
A·J·拉切尔
G·辛格
J·坝基
J·罗布尔
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Lonza AG
Original Assignee
Lonza AG
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza AG filed Critical Lonza AG
Publication of CN116218782A publication Critical patent/CN116218782A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0008—Oxidoreductases (1.) acting on the aldehyde or oxo group of donors (1.2)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1075—Isolating an individual clone by screening libraries by coupling phenotype to genotype, not provided for in other groups of this subclass
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0681—Cells of the genital tract; Non-germinal cells from gonads
    • C12N5/0682—Cells of the female genital tract, e.g. endometrium; Non-germinal cells from ovaries, e.g. ovarian follicle cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0012—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7)
    • C12N9/0026—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5)
    • C12N9/0028—Oxidoreductases (1.) acting on nitrogen containing compounds as donors (1.4, 1.5, 1.6, 1.7) acting on CH-NH groups of donors (1.5) with NAD or NADP as acceptor (1.5.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y105/00—Oxidoreductases acting on the CH-NH group of donors (1.5)
    • C12Y105/01—Oxidoreductases acting on the CH-NH group of donors (1.5) with NAD+ or NADP+ as acceptor (1.5.1)
    • C12Y105/01012—1-Pyrroline-5-carboxylate dehydrogenase (1.5.1.12)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2510/00—Genetically modified cells
    • C12N2510/02—Cells for production

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Reproductive Health (AREA)
  • Cell Biology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
CN202310027006.7A 2018-02-02 2019-02-01 细胞选择和修饰细胞代谢的方法 Pending CN116218782A (zh)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US201862625773P 2018-02-02 2018-02-02
US62/625,773 2018-02-02
CN201980011543.4A CN111971388B (zh) 2018-02-02 2019-02-01 细胞选择和修饰细胞代谢的方法
PCT/US2019/016403 WO2019152876A2 (en) 2018-02-02 2019-02-01 Methods of cell selection and modifying cell metabolism

Related Parent Applications (1)

Application Number Title Priority Date Filing Date
CN201980011543.4A Division CN111971388B (zh) 2018-02-02 2019-02-01 细胞选择和修饰细胞代谢的方法

Publications (1)

Publication Number Publication Date
CN116218782A true CN116218782A (zh) 2023-06-06

Family

ID=65904520

Family Applications (2)

Application Number Title Priority Date Filing Date
CN202310027006.7A Pending CN116218782A (zh) 2018-02-02 2019-02-01 细胞选择和修饰细胞代谢的方法
CN201980011543.4A Active CN111971388B (zh) 2018-02-02 2019-02-01 细胞选择和修饰细胞代谢的方法

Family Applications After (1)

Application Number Title Priority Date Filing Date
CN201980011543.4A Active CN111971388B (zh) 2018-02-02 2019-02-01 细胞选择和修饰细胞代谢的方法

Country Status (7)

Country Link
US (2) US12077786B2 (https=)
EP (2) EP3741853B1 (https=)
JP (4) JP7108036B2 (https=)
CN (2) CN116218782A (https=)
ES (2) ES2871818T3 (https=)
GB (1) GB2577236A (https=)
WO (1) WO2019152876A2 (https=)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115029307A (zh) * 2022-07-28 2022-09-09 吉林大学 一种延缓MSCs衰老的方法

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP3741853B1 (en) 2018-02-02 2023-11-22 Lonza Ltd. Methods of cell selection and modifying cell metabolism
US20220403398A1 (en) 2019-11-14 2022-12-22 Lonza Ltd Methods of cell selection
CN114681386A (zh) * 2022-03-16 2022-07-01 和携科技有限公司 一种动物来源外泌体组合物及其在制备皮肤抗衰老和抗过敏制剂中的应用
CN120283058A (zh) * 2022-09-30 2025-07-08 西格马-奥尔德里奇有限责任公司 经由甘氨酸-甲酸盐生物合成途径的代谢选择
EP4594506A1 (en) * 2022-09-30 2025-08-06 Sigma-Aldrich Co., LLC Metabolic selection via the serine biosynthesis pathway
CN116970559B (zh) * 2023-09-21 2023-11-28 派能生物科技（深圳）有限公司 一种制备脂肪间充质干细胞因子的诱导培养基及其应用
WO2026062080A1 (en) * 2024-09-17 2026-03-26 Ares Trading S.A. Cell having increased secretion capabilities and method of use

Family Cites Families (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5131837Y2 (https=) 1973-12-13 1976-08-09
JPH0672B2 (ja) * 1984-11-20 1994-01-05 味の素株式会社 発酵法によるｌ―フェニルアラニンまたはｌ―チロシンの製造法
GB9022545D0 (en) 1990-10-17 1990-11-28 Wellcome Found Culture medium
IT1258959B (it) 1992-06-09 1996-03-11 Impianto a moduli mobili per lo sviluppo e la produzione di prodotti biotecnologici su scala pilota
US5344923A (en) * 1992-09-29 1994-09-06 The Ohio State University Research Foundation Nucleotide sequence encoding for bifunctional enzyme for proline production
EP1958962A3 (en) 1997-06-12 2013-05-01 Novartis International Pharmaceutical Ltd. Artificial antibody polypeptides
DE19919124A1 (de) * 1999-04-27 2000-11-23 Rhein Biotech Proz & Prod Gmbh Nukleinsäuremolekül, umfassend eine für ein Polypeptid mit Chorismatmutase-Aktivität kodierende Nukleinsäure
JP2001054384A (ja) * 1999-08-17 2001-02-27 Hitachi Ltd シロイヌナズナ・δ１−ピロリン−５−カルボン酸合成酵素遺伝子のプロモーター。
HK1047109A1 (zh) 1999-10-15 2003-02-07 University Of Massachusetts 作为指定基因干预工具的rna干预轨迹基因
US6326193B1 (en) 1999-11-05 2001-12-04 Cambria Biosciences, Llc Insect control agent
AU2001275474A1 (en) 2000-06-12 2001-12-24 Akkadix Corporation Materials and methods for the control of nematodes
DE60210697T2 (de) * 2001-02-13 2007-04-05 Ajinomoto Co., Inc. Verfahren zur Produktion von L-Aminosäuren mittels Bakterien der Gattung Escherichia
US8298054B2 (en) 2004-02-03 2012-10-30 Xcellerex, Inc. System and method for manufacturing
DK1773976T4 (da) 2004-06-04 2020-02-10 Global Life Sciences Solutions Usa Llc Engangsbioreaktorsystemer og -fremgangsmåder
WO2007067656A2 (en) 2005-12-05 2007-06-14 Hope Ernest G Prevalidated, modular good manufacturing practice-compliant facility
JP2010524467A (ja) 2007-04-16 2010-07-22 モメンタ ファーマシューティカルズ インコーポレイテッド 規定の糖タンパク質産物および関連の方法
WO2009072118A1 (en) * 2007-12-04 2009-06-11 Yeda Research And Development Co. Ltd. Transgenic plants having altered levels of aromatic amino acids and metabolites derived therefrom
SG10201402634SA (en) * 2009-05-26 2014-09-26 Agency Science Tech & Res Muteins of the pyrroline-5-carboxylate reductase 1
WO2011139708A2 (en) 2010-04-26 2011-11-10 Toyota Motor Engineering & Manufacturing North America, Inc. Improved hydrogen release from complex metal hydrides by solvation in ionic liquids
US9234210B2 (en) * 2010-08-25 2016-01-12 Intrexon Ceu, Inc. Selectable markers and related methods
US10371394B2 (en) 2010-09-20 2019-08-06 Biologics Modular Llc Mobile, modular cleanroom facility
US9388373B2 (en) 2011-03-08 2016-07-12 University Of Maryland Baltimore County Microscale bioprocessing system and method for protein manufacturing
EP2700713B1 (en) 2012-08-21 2016-07-13 Miltenyi Biotec GmbH Screening and enrichment system for protein expression in eukaryotic cells using a tricistronic expression cassette
EP3741853B1 (en) 2018-02-02 2023-11-22 Lonza Ltd. Methods of cell selection and modifying cell metabolism

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115029307A (zh) * 2022-07-28 2022-09-09 吉林大学 一种延缓MSCs衰老的方法

Also Published As

Publication number Publication date
JP7404453B2 (ja) 2023-12-25
GB2577236A (en) 2020-03-18
JP7108036B2 (ja) 2022-07-27
JP2022160453A (ja) 2022-10-19
WO2019152876A3 (en) 2019-11-07
JP2024037889A (ja) 2024-03-19
US12077786B2 (en) 2024-09-03
EP3645720A2 (en) 2020-05-06
EP3645720B1 (en) 2021-03-10
GB202001194D0 (en) 2020-03-11
ES2972544T3 (es) 2024-06-13
ES2871818T3 (es) 2021-11-02
US20230174971A1 (en) 2023-06-08
EP3741853A1 (en) 2020-11-25
WO2019152876A2 (en) 2019-08-08
JP2021511781A (ja) 2021-05-13
CN111971388A (zh) 2020-11-20
JP2026048662A (ja) 2026-03-17
US20240401003A1 (en) 2024-12-05
CN111971388B (zh) 2023-01-31
EP3741853B1 (en) 2023-11-22

Similar Documents

Publication Publication Date Title
CN111971388B (zh) 细胞选择和修饰细胞代谢的方法
CN111372946B (zh) 难以表达的蛋白质的多位点特异性整合细胞
CN110785494B (zh) 用于生产生物制品的通用自调节哺乳动物细胞系平台
JP7678805B2 (ja) 細胞の選択方法
CN118995660A (zh) 突变型piggyBac转座酶
TR201802431T4 (tr) Protein üretme usulü.
JP2024161560A (ja) 内因性タンパク質のレベルを低下させることによって生物学的産物の産生を改善する方法
HK40090181A (zh) 细胞选择和修饰细胞代谢的方法
HK40070504A (en) Methods of cell selection
HK40019706B (zh) 用於生产生物制品的通用自调节哺乳动物细胞系平台
HK40047568B (zh) 用於通过降低内源性蛋白质的水平来改善生物产物的生产的方法

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
REG Reference to a national code

Ref country code: HK

Ref legal event code: DE

Ref document number: 40090181

Country of ref document: HK