CN116173111A - Chinese herbal compound preparation for preventing and treating pullorum disease and preparation method thereof - Google Patents

Abstract

The invention discloses a traditional Chinese medicine compound preparation for preventing and treating pullorum disease and a preparation method thereof, and particularly relates to the technical field of veterinary medicines, wherein the traditional Chinese medicine compound preparation comprises the following raw materials in parts by weight: 20-40 parts of pulsatilla chinensis, 20-40 parts of scutellaria baicalensis, 10-30 parts of dandelion, 10-30 parts of purslane, 10-30 parts of myrobalan, 5-15 parts of astragalus membranaceus and 5-15 parts of dried orange peel; weighing the traditional Chinese medicines according to the prescription proportion, adding distilled water without the medicinal materials into a marmite, soaking the traditional Chinese medicines in the marmite for 30min, boiling with strong fire, decocting with slow fire for 30min, filtering the liquid medicine into a distillation flask with eight layers of gauze, and preparing the liquid medicine under the conditions of 0.9MPa and 60 ℃. The traditional Chinese medicine formula has the effects of clearing away heat and toxic materials, eliminating dampness and cooling blood, astringing intestines and relieving diarrhea, tonifying qi and regulating qi, and the raw materials of the traditional Chinese medicine formula are only traditional Chinese medicines, so that crude drug components of the traditional Chinese medicine formula are rapidly discharged out of the body along with excrement except for nearly thoroughly clearing possible strains contained in the spleen and the duodenal bacteria of the chicks, and the chicks after healing are ensured to eat and develop normally.

Description

Chinese herbal compound preparation for preventing and treating pullorum disease and preparation method thereof

Technical Field

The invention relates to the technical field of veterinary medicines, in particular to a traditional Chinese medicine compound preparation for preventing and treating pullorum disease and a preparation method thereof.

Background

Pullorum disease (pu l l orum d i sease, hereinafter referred to as PD) is a bacterial infectious disease caused by salmonella, the size of salmonella pullorum is 0.3X0.5-1X 2.5 mu m, the salmonella pullorum is gram-negative bacteria, straight-bar bacteria with blunt ends, no capsule and no spore, and the salmonella pullorum cannot move. The bacteria survive for months to years in contaminated waste, feed, water and other beneficial environments. PD is one of common infectious diseases which are caused by Salmonella pullorum and harm to poultry farming, and can cause chicken diseases of different age groups, chicks usually occur in 1-2 weeks old and are in the form of white pasty and unshaped feces, the feces of serious people can stick to anus, and the feces are often emitted as sharp sounds due to pain; the bred chickens usually occur at 40-80 days of age and are mainly characterized by symptoms such as listlessness, diarrhea, anorexia and the like. Adult chickens are generally recessive infections, which are mainly manifested by damaged reproductive organs, and reduced laying rate and hatching rate. The chicks often show septicemia, insufficient yolk absorption, hepatomegaly and visible punctate bleeding or gray needle-like necrosis points after the change of the section; spleen enlargement and fragile texture; the intestinal tract presents catarrhal inflammation, and the cecum has cheese-like substances to block the intestinal tract. The developed chicken has common hepatomegaly, about 2-3 times of normal hepatomegaly, and the whole abdominal cavity is almost covered by the liver. Adult chickens mainly occur in the reproductive system and are characterized by abnormal colors such as underdeveloped ovaries, deformed follicles, gray or yellow gray ova.

Currently, there is no effective immunization and radical therapy for PD. Antibiotics are mostly adopted for treating PD, but antibiotics are applied for a long time and in a large quantity, so that the drug resistance of poultry is enhanced; producing toxic and side effects; and antibiotics remain in animals, thereby threatening the health of humans. The traditional Chinese medicine has the advantages of no residue, no drug resistance, small toxic and side effects and the like, and is gradually applied to the aquaculture industry.

By combining with the differentiation of symptoms of traditional Chinese medicine, the pullorum disease belongs to diarrhea, and is caused by the invasion of damp-heat by animal organisms, so that the damp-heat is forced into intestinal tracts, and qi movement is blocked to cause diarrhea, so that the times of defecation are increased, even the adverse effect of dredging and descending is caused, qi and blood stasis are caused, the intestinal collaterals are damaged, and the animal organisms are putrefactive into purulent blood to cause dysentery with reddish white.

In the prior art, although medicines for treating pullorum disease are developed based on traditional Chinese medicines, thereby preventing and treating pullorum disease, and avoiding the generation of drug-resistant strains and the residue of antibiotics in chicken bodies, the addition of extra ecological agents and enzymes is added into the raw materials, although the strain residue of intestinal tracts is improved to a certain extent, the medicine residue is caused in the intestinal tracts, and the feeding condition and the weight increasing condition of chickens are improved in the initial stage and the process of taking the medicines, but the conditions of slow weight increasing progress or negative increase are caused after the subsequent feeding interruption, which is unfavorable for the growth of the chickens after the treatment.

Disclosure of Invention

In order to overcome the defects in the prior art, the embodiment of the invention provides a traditional Chinese medicine compound preparation for preventing and treating pullorum disease and a preparation method thereof, and the technical problems to be solved by the invention are as follows: how to improve the existing pullorum disease treatment drugs to stay in intestinal tracts and influence the weight gaining condition of chicks after the drug interruption.

In order to achieve the above purpose, the present invention provides the following technical solutions: a traditional Chinese medicine compound preparation for preventing and treating pullorum disease comprises the following raw materials in parts by weight: 20-40 parts of pulsatilla chinensis, 20-40 parts of scutellaria baicalensis, 10-30 parts of dandelion, 10-30 parts of purslane, 10-30 parts of myrobalan, 5-15 parts of astragalus membranaceus and 5-15 parts of dried orange peel.

In a preferred embodiment, the material comprises the following raw materials in parts by weight: 20 parts of pulsatilla chinensis, 20 parts of scutellaria baicalensis, 10 parts of dandelion, 30 parts of purslane, 30 parts of myrobalan, 15 parts of astragalus membranaceus and 15 parts of dried orange peel.

In a preferred embodiment, the material comprises the following raw materials in parts by weight: 30 parts of pulsatilla chinensis, 30 parts of scutellaria baicalensis, 20 parts of dandelion, 20 parts of purslane, 20 parts of myrobalan, 10 parts of astragalus membranaceus and 10 parts of dried orange peel.

In a preferred embodiment, the material comprises the following raw materials in parts by weight: 40 parts of pulsatilla chinensis, 40 parts of scutellaria baicalensis, 30 parts of dandelion, 10 parts of purslane, 10 parts of myrobalan, 5 parts of astragalus membranaceus and 5 parts of dried orange peel.

The invention also discloses a preparation method of the traditional Chinese medicine compound preparation for preventing and treating pullorum disease, which comprises the following specific steps:

s1, preparing raw materials: weighing the traditional Chinese medicines according to the prescription proportion, adding distilled water without the medicinal materials into a marmite, and soaking the traditional Chinese medicines in the marmite for 30min;

s2, decocting: boiling the medicinal materials soaked in the step S1 by using strong fire, and decocting with slow fire for 30min;

s3, filtering: stacking and filtering the liquid medicine decocted in the step S2 into a distillation flask by using eight layers of 80-120 mesh gauze;

s4, concentrating: and (3) preparing the filtered liquid medicine in the step (S3) into crude drug with the concentration of 1g/mL at the temperature of 60 ℃ under the pressure of 0.9MPa by using a vacuum pump and a rotary evaporator for standby.

The medicine property and pharmacology of each traditional Chinese medicine component are as follows:

pulsatilla root is often used as a main medicine for treating PD, and is cold in nature and bitter in taste; the Chinese medicinal composition has the effects of invigorating stomach and large intestine, relieving diarrhea with astringents, clearing heat and detoxicating, cooling blood and stopping diarrhea, inhibiting bacillus dysenteriae and astringing intestinal mucosa, and relieving diarrhea and sterilizing PD;

baical skullcap root, radix Scutellariae, which has cold property and bitter taste, enters lung, gall bladder, spleen, large intestine and small intestine channels, and has the effects of clearing heat and drying dampness, purging pathogenic fire and removing toxin, and stopping bleeding;

dandelion has the effects of clearing heat and detoxicating, detumescence and resolving hard mass, enters liver channels, and has a definite target spot for the liver of a sick chicken infected with PD;

purslane is cold in nature, sour in taste, enters liver and large intestine meridians, and sour can astringe, so that purslane is a common medicament for treating diarrhea;

the chebula fruit has flat property, bitter, sour and astringent taste, can astringe large intestine, stop diarrhea, and is suitable for long-term diarrhea and other symptoms;

astragalus root has warm nature, sweet taste, and lung and spleen meridian return, and has the effects of tonifying qi and yang, tonifying defensive and consolidating superficial resistance, inducing diuresis and reducing edema;

pericarpium Citri Reticulatae is warm in nature, bitter and pungent in flavor; has effects of regulating qi-flowing, invigorating spleen, eliminating dampness and resolving phlegm.

The invention has the technical effects and advantages that:

the pulsatilla root and the baikal skullcap root in the invention are used as monarch drugs for clearing heat and detoxicating, and drying dampness and cooling blood; dandelion, purslane and myrobalan can assist the monarch drugs in clearing heat and detoxicating, astringing intestines and stopping diarrhea, and are ministerial drugs; the astragalus and the dried orange peel supplement qi and regulate qi to be used as adjuvant and guiding drugs; the medicines are combined together to play roles of clearing heat and detoxicating, eliminating dampness and relieving diarrhea; the traditional Chinese medicine formula is prepared from traditional Chinese medicinal materials, and has no additional additives or biological enzymes, and besides the strains possibly contained in chicken spleen and duodenal bacteria are removed approximately and thoroughly, crude drug components of the traditional Chinese medicine formula are rapidly discharged out of the body along with excrement, so that the relapse of pullorum after treatment is avoided, the feeding and normal development of the chicken after treatment are ensured, the subsequent growth and development of the chicken after treatment are ensured, the problems of anorexia, slow speed increase, weight reduction and the like of the chicken after treatment are avoided, and the traditional Chinese medicine formula is beneficial to promoting green antibiotic-free cultivation.

Drawings

FIG. 1 is a bar graph showing the weight gain results of individual groups of chicks according to the present invention.

FIG. 2 is a bar graph showing liver index results for each group of chicks according to the present invention.

FIG. 3 is a bar graph showing spleen index results of individual groups of chicks according to the present invention.

Fig. 4 is a bar graph showing the liver fungus load results of each group of chicks according to the present invention.

Fig. 5 is a bar graph showing the results of the spleen bacterial load of each group of chicks of the present invention.

FIG. 6 is a bar graph showing the results of the duodenal load of each group of chicks of the present invention.

Fig. 7 is a bar graph showing the results of cecum load for each group of chicks of the present invention.

Detailed Description

The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

The present invention will be described in further detail with reference to the following examples for the purpose of making the object of the present invention more specifically clear. The described embodiments of the invention are only some, but not all, embodiments of the invention.

Clinical efficacy test of the invention

Embodiment one:

(1) Preparation of bacterial liquid

Taking out CVCC1789 strain from a refrigerator at-80 ℃, operating among bacteria, sucking 10 mu L of the strain out by a pipetting gun, placing the strain in a conical flask filled with 100mL of tryptone soybean broth culture medium (TSB), shaking the strain for 12 hours at the temperature of 37 ℃ by a shaking table at the rotating speed of 180rpm to activate the strain, sucking 50 mu L of the strain from the activated strain liquid, uniformly coating the strain on a Maiconkai culture medium by a coating rod, culturing the strain in the incubator at the temperature of 37 ℃ for 18-24 hours, observing that smaller colorless or amber colonies are generated on the Maiconkai culture medium, picking typical single colonies to carry out gram staining, and observing round red two ends under a microscope, namely the short rod-shaped bacteria, namely Salmonella pullorum; selecting single colony in 50mL TSB culture medium, shaking at 37deg.C with 180rpm rotary table for 12 hr, sucking 2mL of shaking bacterial liquid between bacteria, measuring absorbance at enzyme marker, sucking 100 μL of uniform coating on Maikang Kai culture medium with pipette, and repeating for 3 times for each concentration; culturing in a constant temperature incubator at 37 ℃ for 18-24 hours, and performing plate counting; finally, the concentration of the bacterial liquid is regulated to be 2.5x108CFU/0.2mL;

(2) PD model establishment

50 healthy sea-blue brown chickens with 1 day age are purchased from the national academy of sciences of Changchun, and each chicken is irrigated with 2.5x108CFU/0.2mL of bacterial liquid, and the amount of the irrigated stomach is 0.2 mL/chicken; 20 chickens after the toxin is attacked have the phenomena of mental wilting, low head, sagging wings, white pasty pull, non-forming feces, and a few sick chickens have the feces adhesion to anus; 24 hours after the toxicity attack, 45 chickens have the phenomena of mental wilting, low head necking, wing sagging, white pasty drawing, non-formed feces, and few sick chickens have the phenomenon that the feces adhere to anus; any 10 chickens are selected from 45 chickens with clinical symptoms for carrying out the section examination, the section examination finds that the liver and spleen are swollen, the liver and spleen have needlepoint-shaped gray-white dead spots, the disease tissues are ground and coated on a Maiconkai culture medium, a constant temperature incubator at 37 ℃ is used for culturing for 18-24 hours to see gray-white or colorless colonies, single colonies are selected for gram staining, and red short rod-shaped bacteria are observed under a microscope, which indicates that the molding is successful.

Embodiment two:

(1) Preparation of liquid medicine

Accurately weighing 30 g of pulsatilla chinensis, 30 g of scutellaria baicalensis, 20 g of dandelion, 20 g of purslane, 20 g of myrobalan, 10 g of astragalus membranaceus and 10 g of dried orange peel; wrapping the Chinese medicinal materials with three layers of gauze, placing into a marmite, adding distilled water without the Chinese medicinal materials into the marmite, soaking for 30min, boiling with strong fire, decocting with slow fire for 30min, filtering the decoction with eight layers of gauze, pouring the filtered decoction into a round bottom flask, and preparing into crude drug with concentration of 1g/mL under the conditions of 0.9MPa and 60deg.C with vacuum pump and rotary evaporator;

(2) Preparation of bacterial liquid

The operation steps are the same as in (1) in the first embodiment;

(3) Drug efficacy test of traditional Chinese medicine compound preparation on PD

140 sea-sky-brown chickens with 1 day old are purchased from a farm of the vinca, raised to 7 days old and have good growth conditions, and 140 chickens are randomly divided into 7 groups, namely a blank group, a model group, a 2g/kg preventive group, a 1g/kg low-dose treatment group, a 2g/kg medium-dose treatment group, a 4g/kg high-dose treatment group and a western medicine group (amoxicillin soluble powder); molding the other groups according to the method except that the blank group is filled with 0.2mL of physiological saline, wherein the concentration of the bacterium liquid filled into the stomach of each chicken is 2.5x108CFU/0.2mL; the preventive group is administrated once a day at 5 days of age, for 5 days, and the preventive group, the low-dose therapeutic group, the medium-dose therapeutic group and the high-dose therapeutic group are administrated by 2g/kg, 1g/kg, 2g/kg and 4g/kg of stomach-lavage respectively, once a day, for 5 days; the western medicine group is administrated by stomach irrigation, the administration dosage is 0.4g/kg, once a day, and the administration lasts for 5 days; each group freely ingests drinking water during the test period, and after 5 days of administration, the water is observed for 5 days for carrying out the section inspection;

recording clinical symptoms of each group of chickens, such as mental state, body temperature and weight, feces, drinking water and the like, every day after the toxin is attacked; changes in dissections, such as liver, spleen, duodenum, cecum, etc.; scoring clinical symptoms and profile changes of each group of chickens one day before profile, scoring criteria as in tables 1 and 2, and scoring statistics for each group of clinical symptoms and profile changes as in tables 3 and 4;

TABLE 1 clinical symptom scoring criteria

Table 2 section scoring criteria

Table 3 statistics of clinical symptom scores for each group

Table 4 shows the values of the section change

Combining the data in tables 1-4 to obtain the therapeutic effect of the Chinese medicinal preparation on PD

Clinical symptoms

All chicken mental states in the blank group are fed with water, and the fecal conditions are normal; the model group has the phenomena that all chickens have mental wilt, low head necking, wing sagging, white pasty and unshaped feces, and few sick chickens have the feces adhered to anus; the clinical symptoms of 90% of chickens are relieved to different degrees after the treatment of the prevention group; the low dose treatment group had 70% reduction in clinical symptoms in chickens; the clinical symptoms of 85% of chickens in the medium-dose treatment group are relieved to different degrees; the high dose treatment group had 75% of the clinical symptoms of chickens reduced to varying degrees; the clinical symptoms of 70% of chickens are relieved to different degrees after the western medicine group is treated;

changes in dissecting examination

The chicken profile inspection conditions of the blank group are normal, the typical profile inspection changes of all chickens in the model group are different, the profile inspection changes of 90% of chickens in the prevention group are reduced to different degrees, the profile inspection changes of 65% of chickens in the low-dose treatment group are reduced to different degrees, the profile inspection changes of 80% of chickens in the medium-dose treatment group are reduced to different degrees, the profile inspection changes of 70% of chickens in the high-dose treatment group are reduced to different degrees, and the profile inspection changes of 90% of chickens in the western group are reduced to different degrees;

using One-way ANOVA analysis data, graph Pad Pr i sm 8.0.0 plotted, representing significant differences P <0.05 compared to model group; * Represents that the difference was very significant P < 0.01 compared to the model group;

wherein: c: blank group, M: model group, prevention: traditional Chinese medicine prevention group, low: low dose treatment group, in: medium dose treatment group, high: high dose treatment group, western medicine: western medicine group.

Prevention and treatment effect of traditional Chinese medicine compound preparation on PD

The results were as follows:

(1) Weight gain of chicks of each group

As a result, as shown in fig. 1, the weight gain of the blank group, the preventive group and the middle-dose treatment group is extremely remarkably increased (P < 0.01) compared with the weight gain of the model group; the weight of the low-dose treatment group, the high-dose treatment group and the western medicine group is obviously increased (P is less than 0.05);

(2) Liver index results for groups of chicks

As a result, as shown in fig. 2, the liver index of the blank group was extremely significantly reduced (P < 0.01) compared to the model group; the liver index of the preventive group, the middle-dose treatment group and the western medicine group is obviously lower than that of the model group (P is less than 0.05);

(3) Spleen index results for each group of chicks

As shown in fig. 3, the spleen index of the blank group and the western medicine group is extremely remarkably reduced (P < 0.01) compared with the model group;

(4) Liver bacterial load results for each group of chicks

As shown in fig. 4, the bacterial load of the liver of the blank group is set to be 0, and compared with the model group, the bacterial load of the blank group, the preventing group, the low-dose treatment group, the medium-dose treatment group, the high-dose treatment group and the western medicine group is extremely obviously reduced (P is less than 0.01);

(5) Results of spleen bacterial load of chicks of each group

As shown in fig. 5, assuming that the spleen bacterial load of the blank group is 0, compared with the model group, the spleen bacterial load of the blank group, the prevention group, the medium-dose treatment group, the high-dose treatment group and the western medicine group is extremely obviously reduced (P is less than 0.01);

(6) Results of duodenal load for each group of chicks

As shown in fig. 6, the duodenal bacterial load of the blank group is set to be 0, and compared with the model group, the duodenal bacterial load of the blank group, the prevention group, the middle-dose treatment group, the high-dose treatment group and the western medicine group is extremely obviously reduced (P is less than 0.01);

(7) Results of cecum load for groups of chicks

As a result, as shown in fig. 7, the cecum load of the blank group was 0, and the cecum loads of the blank group, the prophylaxis group, the medium dose treatment group, and the high dose treatment group were extremely significantly reduced (P < 0.01), and the western group bacterial load was significantly reduced (P < 0.05) as compared with the model group.

Summary and analysis

Except for blank groups, before treatment, each group of chickens had mental wilting, wing drooping and head necking, pulled white paste, formed feces, and sometimes anus had feces adhesion; the medicine is used for 5 days, and after observation for 5 days, the mental state of the chicken is obviously improved, the appetite, the defecation and the necrosed point of the liver and the spleen are obviously reduced, the swelling degree of the liver and the spleen is obviously reduced, the duodenal bleeding, the cecum cheese-like substance, the excrement accumulation and the swelling degree are also obviously reduced, and after treatment, the clinical symptoms and the section detection changes of each group are also reduced to different degrees except the model group; in addition, the weight gain of the prevention group and each treatment group is obviously increased compared with the model group, and the liver indexes of the prevention group, the medium-dose treatment group and the western medicine group are obviously reduced compared with the model group; liver and cecum loads were reduced to different extents in both the prophylaxis group and the low, medium, and high dose treatment groups compared to the model group; spleen and duodenal bacterial load in the prophylaxis group, the medium-dose treatment group, the high-dose treatment group and the western medicine group are reduced to different degrees compared with the model group; among the prevention groups of the traditional Chinese medicine prescription, the prevention group has the best effect, and can replace western medicines, namely amoxicillin soluble powder, to prevent and treat pullorum disease;

in addition, the traditional Chinese medicine formula has the advantages that the cure rate of the traditional Chinese medicine formula for preventing and treating chicken pullorum disease is not lower than 90%, the cure rate of the traditional Chinese medicine formula for treating and preventing chicken pullorum disease is as high as 85%, the cure rate of the traditional Chinese medicine formula for treating and preventing chicken pullorum disease is greatly improved compared with the cure rate of 70% of western medicines, the traditional Chinese medicine formula raw materials are only traditional Chinese medicines, no additional additives or biological enzymes are added, the traditional Chinese medicine formula can be used for nearly completely removing the strains possibly contained in chicken spleen and duodenal bacteria, and due to the characteristics of mildness and easy removal of the traditional Chinese medicine, the crude medicine components of the traditional Chinese medicine formula are rapidly removed from the body along with excrement, so that the possibility of drug-resistant strain breeding caused by coexistence of the strains in the body is effectively prevented, in addition, the feeding and developing of the chicken after being cured are avoided, the feeding and developing of the chicken after being cured are prevented, the feeding and developing of the chicken after being cured are ensured, the subsequent growth of the chicken is prevented, the problems of slow anorexia, the chicken weight loss and the like are avoided.

The last points to be described are: first, in the description of the present application, it should be noted that, unless otherwise specified and defined, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be mechanical or electrical, or may be a direct connection between two elements, and "upper," "lower," "left," "right," etc. are merely used to indicate relative positional relationships, which may be changed when the absolute position of the object being described is changed;

secondly: in the drawings of the disclosed embodiments, only the structures related to the embodiments of the present disclosure are referred to, and other structures can refer to the common design, so that the same embodiment and different embodiments of the present disclosure can be combined with each other under the condition of no conflict;

finally: the foregoing description of the preferred embodiments of the invention is not intended to limit the invention to the precise form disclosed, and any such modifications, equivalents, and alternatives falling within the spirit and principles of the invention are intended to be included within the scope of the invention.

Claims (7)

1. A Chinese herbal compound preparation for preventing and treating pullorum disease is characterized in that: the material comprises the following raw materials in parts by weight: 20-40 parts of pulsatilla chinensis, 20-40 parts of scutellaria baicalensis, 10-30 parts of dandelion, 10-30 parts of purslane, 10-30 parts of myrobalan, 5-15 parts of astragalus membranaceus and 5-15 parts of dried orange peel.

2. The traditional Chinese medicine compound preparation for preventing and treating pullorum disease according to claim 1, which is characterized in that: the material comprises the following raw materials in parts by weight: 20 parts of pulsatilla chinensis, 20 parts of scutellaria baicalensis, 10 parts of dandelion, 30 parts of purslane, 30 parts of myrobalan, 15 parts of astragalus membranaceus and 15 parts of dried orange peel.

3. The traditional Chinese medicine compound preparation for preventing and treating pullorum disease according to claim 1, which is characterized in that: the material comprises the following raw materials in parts by weight: 30 parts of pulsatilla chinensis, 30 parts of scutellaria baicalensis, 20 parts of dandelion, 20 parts of purslane, 20 parts of myrobalan, 10 parts of astragalus membranaceus and 10 parts of dried orange peel.

4. The traditional Chinese medicine compound preparation for preventing and treating pullorum disease according to claim 1, which is characterized in that: the material comprises the following raw materials in parts by weight: 40 parts of pulsatilla chinensis, 40 parts of scutellaria baicalensis, 30 parts of dandelion, 10 parts of purslane, 10 parts of myrobalan, 5 parts of astragalus membranaceus and 5 parts of dried orange peel.

5. A preparation method for preparing a traditional Chinese medicine compound preparation for preventing and treating pullorum disease according to any one of claims 1 to 4, which is characterized in that: the method comprises the following specific steps:

s1, preparing raw materials: weighing the traditional Chinese medicines according to the prescription proportion, adding distilled water without the medicinal materials into a marmite, and soaking the traditional Chinese medicines in the marmite for 30min;

s2, decocting: boiling the medicinal materials soaked in the step S1 by using strong fire, and decocting with slow fire for 30min;

s3, filtering: filtering the liquid medicine decocted in the step S2 into a distillation flask by using eight layers of gauze;

s4, concentrating: and (3) preparing the filtered liquid medicine in the step (S3) into crude drug with the concentration of 1g/mL by using a vacuum pump and a rotary evaporator for standby.

6. The method for preparing the traditional Chinese medicine compound preparation for preventing and treating pullorum disease according to claim 5, which is characterized in that: the gauze in the step S3 is 80-120 meshes, and eight layers of stacked gauze are adopted for filtering.

7. The method for preparing the traditional Chinese medicine compound preparation for preventing and treating pullorum disease according to claim 5, which is characterized in that: the conditions for the concentration step in the step S4 were set to 0.9MPa and 60 ℃.

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