CN116172022A - Plant growth regulator, preparation method and application thereof - Google Patents

Plant growth regulator, preparation method and application thereof Download PDF

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Publication number
CN116172022A
CN116172022A CN202310192997.4A CN202310192997A CN116172022A CN 116172022 A CN116172022 A CN 116172022A CN 202310192997 A CN202310192997 A CN 202310192997A CN 116172022 A CN116172022 A CN 116172022A
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Prior art keywords
plant growth
growth regulator
plant
leaching
preparation
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CN202310192997.4A
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Chinese (zh)
Inventor
赵晓明
柳嘉桐
梁莹
孟欣然
赵迪
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Shenyang University
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Shenyang University
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Priority to CN202310192997.4A priority Critical patent/CN116172022A/en
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    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • A01N65/08Magnoliopsida [dicotyledons]
    • A01N65/12Asteraceae or Compositae [Aster or Sunflower family], e.g. daisy, pyrethrum, artichoke, lettuce, sunflower, wormwood or tarragon
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N65/00Biocides, pest repellants or attractants, or plant growth regulators containing material from algae, lichens, bryophyta, multi-cellular fungi or plants, or extracts thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02ATECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Plant Pathology (AREA)
  • Environmental Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Agronomy & Crop Science (AREA)
  • Mycology (AREA)
  • Microbiology (AREA)
  • Dentistry (AREA)
  • Biotechnology (AREA)
  • Natural Medicines & Medicinal Plants (AREA)
  • Botany (AREA)
  • Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Pest Control & Pesticides (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)
  • Cultivation Of Plants (AREA)

Abstract

The invention belongs to the field of plant growth regulator development, and particularly relates to a plant growth regulator, a preparation method and application thereof. The preparation method of the plant growth regulator comprises the following steps: and (3) obtaining stem and leaf samples of the plant of the chlorophytum comosum, grinding and crushing the stem and leaf samples, then placing the ground and crushed stem and leaf samples in leaching liquor for leaching, filtering the leaching liquor, centrifuging the filtrate, and taking supernatant to obtain the plant growth regulator. The invention also provides application of the plant growth regulator in promoting plant growth and development, including regulation and control effects of promoting seed germination, optimizing root system configuration, increasing leaf area and improving root-to-crown ratio. The plant extract material is easy to obtain, the preparation method is simple, the extraction rate is high, the cost is low, the plant extract material is safe and pollution-free, and the plant extract material can effectively promote the growth and development of plants.

Description

Plant growth regulator, preparation method and application thereof
Technical Field
The invention belongs to the technical field of plant growth regulation, and particularly relates to a plant growth regulator, a preparation method and application thereof.
Background
Various environmental problems are increasingly prominent in the world, pollution problems are endlessly layered, water resources are scarce, effective land areas are smaller and smaller, great influence is caused on agricultural production, and the maximum benefit of limited land production is a target which is always expected to be realized in agricultural production. The plant growth regulator is used to regulate the growth state of plant, and this is one common technological process for stabilizing yield, improving quality and raising crop stress resistance. Although widely used in agriculture, it belongs to a class of agricultural chemicals, which are liable to cause environmental pollution in synthesis and production.
Plant growth regulators in the current market are various in variety and different in effect, and are often required to be applied in a matched mode. Farmers often lack guidance in production to use improperly, which results in poor effects or the occurrence of phytotoxicity to affect crop quality and food safety. Therefore, the search for safe and environment-friendly agricultural production methods is a direction of continuous development of agriculture. The development of natural plant growth regulators with strong comprehensive regulation capability, easy availability and no toxic or side effect is also an urgent need in agricultural production.
Disclosure of Invention
The invention aims to solve the technical problems and overcome the defects and shortcomings in the background art, and provides a plant growth regulator, a preparation method and application thereof.
The invention adopts a key plant raw material as a component of a plant growth regulator: an extract of Chlorophytum comosum.
The preferred pearl chlorophytum comosum of the invention is of the circular leaf variety, green bell (Senecio rowleyanus) is an evergreen ornamental plant of the genus senecio in the family of Compositae. Subtropical regions of the original southwest non-drought contain a large amount of sesquicool guaiacols. The main ingredients are eugenol (Spathulenol 22.9%), chromene B (germacrene B12.4%), laurel (myrcene 12.8%) and viradiflorol (viradiflorol 11%). Has obvious antibacterial effect on microorganisms.
The preparation method of the plant growth regulator comprises the following operation steps:
(1) Obtaining a stem and leaf sample of a chlorophytum comosum-green bell, grinding and crushing;
(2) Leaching the crushed materials in leaching liquor and filtering;
(3) Centrifuging the filtrate, and taking supernatant as the plant growth regulator.
Preferably, the weight ratio of the crushed material to the leaching solution in the step (2) is 1:1 to 1:10, more preferably 1:5. The leaching solution is water. The leaching time is 12 hours to 24 hours.
Preferably, the centrifugation parameter in the step (3) is 8000r/min to 12000r/min, and the centrifugation time is 5min to 15min, more preferably 10000r/min, and 10min.
The invention provides application of the plant growth regulator extracted by the method in the following aspects: (1) promoting seed germination;
(2) Optimizing the root system configuration;
(3) Increasing the blade area;
(4) The root-cap ratio is improved.
Compared with the prior art, the gain effect of the invention is as follows:
the plant growth regulator of the invention is derived from plants, does not need chemical synthesis, and does not influence the environment.
The adopted materials are natural plants, can be widely planted, have strong adaptability, are easy to obtain and have low cost.
The extract of the present invention can be extracted without the need of a chemical organic solvent.
The key components of the invention are extracted from natural plants, and the invention has no toxic or side effect on the plants after application.
The plant growth regulator has multiple and comprehensive plant growth regulating and controlling effects and more optimal plant growth promoting effects.
Detailed Description
The present invention will be described in further detail with reference to specific embodiments in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Examples:
the preparation method of the plant growth regulator comprises the following steps:
(1) Obtaining 100g of a stem and leaf sample of a chlorophytum comosum-green bell, grinding and crushing;
(2) Placing the crushed material into 500ml of ultrapure water, leaching for 15 hours, and filtering;
(3) Centrifuging the filtrate at 10000r/min for 10min, and collecting supernatant as the plant growth regulator. Placing in a refrigerator for standby.
In order to examine the effect of the plant growth regulator on plant growth regulation, tomato, mung bean, capsicum and corn seeds are taken as test samples on the basis of a comparative test, and relevant indexes such as seed germination, root system configuration, leaf area, root cap ratio and the like are measured. The result shows that the growth regulator of the invention can effectively and comprehensively regulate plant development and has obvious promotion effect on plant growth.
The experimental method comprises the following steps:
1. accelerating germination of 200 seeds of tomato, mung bean, capsicum and corn seeds, wherein 100 seeds of each plant are added with 10 mu L/L concentration plant growth regulator, and the other 100 seeds are added with ultrapure water as a control;
2. sowing after accelerating germination of tomato, mung bean, chilli and corn seeds, wherein 20 plants are selected, 10 seedlings of each plant are taken, 20 mu L/L concentration of the plant growth regulator is added, and the other 10 plants are added with ultrapure water as a control, and three physiological indexes of root system configuration, leaf area and root cap ratio are measured after 10 days.
Experimental results:
the germination of seeds was observed and calculated, and the results are shown in table 1:
TABLE 1 Effect of plant growth regulators on seed germination of different plants
Figure BDA0004106301410000041
From the results in table 1, it can be seen that the plant growth regulator can effectively promote germination of seeds and promote germination vigor of seeds.
The root system configuration, leaf area and root cap ratio of different plants were measured and the results are shown in tables 2-4.
TABLE 2 influence of plant growth regulators on different plant root system configurations
Figure BDA0004106301410000051
Note that data represent mean ± standard error, different lower case letters represent significant differences (P < 0.05), the same as below.
TABLE 3 Effect of plant growth regulators on different plant leaf areas
Figure BDA0004106301410000052
TABLE 4 Effect of plant growth regulators on different plant root cap ratios
Figure BDA0004106301410000053
From the results in tables 2-4, it can be seen that the plant growth regulator has better regulation and control effects on the aspects of optimizing root system configuration, increasing blade area, improving root cap ratio and the like.
The above description is only of a preferred embodiment of the invention and the invention is not limited to the examples given above. It should be understood that all equivalents and obvious modifications made by any person skilled in the art under the guidance of the present specification are intended to be protected by the present invention.

Claims (6)

1. The plant growth regulator is characterized by comprising an extract of the chlorophytum comosum.
2. A plant growth regulator according to claim 1, wherein the extract is obtained from the species chlorophytum comosum circular leaf-green bell.
3. The method for preparing a plant growth regulator according to claim 1, characterized by comprising the following steps:
(1) Obtaining stems and leaves of the pearl chlorophytum comosum, grinding and crushing;
(2) Leaching the crushed materials in leaching liquor and filtering;
(3) Centrifuging the filtrate, and taking supernatant as the plant growth regulator.
4. A method of preparing a plant growth regulator according to claim 3, wherein the weight ratio of crushed material to leaching solution in step (2) is from 1:1 to 1:10, more preferably 1:5, preferably the leaching solution is water, more preferably the leaching time is from 12 hours to 24 hours.
5. A method of preparing a plant growth regulator according to claim 3, wherein the centrifugation parameters in step (3) are 8000r/min to 12000r/min, and the centrifugation time is 5min to 15min, more preferably 10000r/min,10min.
6. Use of a plant growth regulator obtained by extraction according to the method of any one of claims 3 to 5 for regulating plant growth in at least one of the following ways:
(1) Promoting germination of seeds;
(2) Optimizing the root system configuration;
(3) Increasing the blade area;
(4) The root-cap ratio is improved.
CN202310192997.4A 2023-03-03 2023-03-03 Plant growth regulator, preparation method and application thereof Pending CN116172022A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202310192997.4A CN116172022A (en) 2023-03-03 2023-03-03 Plant growth regulator, preparation method and application thereof

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202310192997.4A CN116172022A (en) 2023-03-03 2023-03-03 Plant growth regulator, preparation method and application thereof

Publications (1)

Publication Number Publication Date
CN116172022A true CN116172022A (en) 2023-05-30

Family

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Family Applications (1)

Application Number Title Priority Date Filing Date
CN202310192997.4A Pending CN116172022A (en) 2023-03-03 2023-03-03 Plant growth regulator, preparation method and application thereof

Country Status (1)

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CN (1) CN116172022A (en)

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