CN116114421A - Method for improving loess by using lactic acid-rich solution to promote plant growth - Google Patents
Method for improving loess by using lactic acid-rich solution to promote plant growth Download PDFInfo
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- CN116114421A CN116114421A CN202310057275.8A CN202310057275A CN116114421A CN 116114421 A CN116114421 A CN 116114421A CN 202310057275 A CN202310057275 A CN 202310057275A CN 116114421 A CN116114421 A CN 116114421A
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- lactic acid
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01B—SOIL WORKING IN AGRICULTURE OR FORESTRY; PARTS, DETAILS, OR ACCESSORIES OF AGRICULTURAL MACHINES OR IMPLEMENTS, IN GENERAL
- A01B79/00—Methods for working soil
- A01B79/02—Methods for working soil combined with other agricultural processing, e.g. fertilising, planting
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
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Abstract
The invention discloses a method for improving loess by using a lactic acid-rich solution to promote plant growth, which comprises the following steps: loess treatment; preparing a lactic acid-rich solution; the method comprises the steps of improving loess cultivation, wherein the lactic acid-rich solution is a solution with the lactic acid content not lower than 1g/L, the proportion of L-lactic acid in lactic acid not lower than 80%, the suspended matter content lower than 2g/L and the pH value between 3.0 and 4.2. The invention can popularize the application of the waste fermentation broth as the loess soil conditioner, evaluates the soil improvement effect through the plant growth condition, has reasonable step sequence and has great application prospect.
Description
Technical Field
The invention relates to the field of loess improvement, in particular to the field of organic acid improvement of loess, and relates to a method for improving loess by using a lactic acid-rich solution to promote plant growth.
Background
Loess has low organic matter content and relatively barren soil. Excessive grazing and improper agricultural farming can lead to vegetation destruction and soil degradation, which poses a threat to loess area soil quality and soil ecosystem sustainability. In this regard, loess quality can be improved by increasing the activity and diversity of soil microorganisms, particularly soil rhizosphere microorganisms. Soil amendments represented by organic acids can enhance the available nutrients of soil and promote the proliferation of rhizosphere microorganisms.
However, organic acids produced by the petrochemical industry are expensive. Inexpensive organic acids can be produced by fermentation processes. Organic acids such as lactic acid, acetic acid, propionic acid and butyric acid are easily obtained from anaerobic digestion of kitchen wastes and kitchen wastes. Accordingly, attempts have been made to utilize such materials as soil amendments to increase the available nutrients of the soil.
At present, researches show that although conditioning agents such as compost, biogas residues and biochar have a certain effect on improving loess quality. However, these conditioning agents are generally directly added to the soil at one time, and are affected by the natural environment, so that the growth of beneficial microorganisms in the soil is slow, and even the beneficial microorganisms are competing with each other, so that the improvement effect is very little and the loess improvement is possibly unfavorable.
Especially lactic acid can be prepared in the long-distance transportation process of kitchen waste or in the raw material ensilage process. And, lactic acid volatility is low, which will reduce transportation consumption and the potential for environmental volatile contamination. The inventor experiment researches find that the easily available lactic acid can promote the development of specific rhizosphere microorganisms in soil, regulate the interaction of the rhizosphere microorganisms and plants, and promote the synthesis of specific volatile plant metabolites for plant growth and information transmission, thereby promoting the plant growth, improving the plant immunity, reducing plant diseases and insect pests and improving the plant growth quantity.
Therefore, the invention provides a technical method for improving loess to promote plant growth by using a lactic acid-rich solution.
Disclosure of Invention
Because of the defects in the prior art, the invention provides a method for improving loess by using a lactic acid-rich solution to promote plant growth, which has practical significance for loess improvement and biomass waste recycling.
In order to achieve the above object, the present invention provides the following technical solutions:
a method for improving loess to promote plant growth using a lactic acid rich solution, the method comprising the steps of: (1) loess treatment; (2) preparing a lactic acid-rich solution; (3) improved loess cultivation; wherein the lactic acid-rich solution refers to a solution with the lactic acid content not lower than 1g/L, the proportion of L-lactic acid in lactic acid not lower than 80%, the suspended matter content lower than 2g/L and the pH value between 3.0 and 4.2.
In one embodiment of the present invention, the loess treatment in step (1) means: firstly, removing animal and plant and gravel residues in a raw loess sample; the soil was then screened and, after three to five days of air drying, the soil sample was placed into a container.
In one embodiment of the invention, in step (1), preferably, the depth of the container is not more than 40cm; more preferably, each container is filled with soil in an amount of 2/3 to 3/4 of the container volume.
In one embodiment of the present invention, in the step (2), the lactic acid-rich solution is preferably a pretreated raw material derived from biomass waste sources, such as kitchen waste fermentation broth, straw silage leachate, and the like.
In one embodiment of the present invention, the lactic acid rich solution is diluted to 410-4110mg-C/L before being used for the modified loess culture in step (3).
In one embodiment of the present invention, in the step (3), the amount of lactic acid applied is 0.07-0.75 mg-C/g-loess in terms of lactic acid when the loess is cultivated.
In one embodiment of the present invention, in the step (3), the specific process of the modified loess cultivation is as follows: placing loess prepared with the lactic acid rich solution in a greenhouse with temperature of 25+ -2deg.C and humidity of 55% + -7%, and culturing in dark place for 5-7 days, wherein 28-57g/kg loess is added daily during culturing period to obtain the final product.
The invention also provides the improved loess prepared by the method.
The invention also provides application of the improved loess in plant cultivation.
Said invention has the following advantages or beneficial effects:
(1) The inventor experiment researches find that the easily available lactic acid can promote the development of specific rhizosphere microorganisms in soil, regulate the interaction of the rhizosphere microorganisms and plants, and promote the synthesis of specific volatile plant metabolites for plant growth and information transmission, thereby promoting the plant growth, improving the plant immunity, reducing plant diseases and insect pests and improving the plant growth quantity. So that the addition of lactic acid can be used to modify loess. And the lactic acid can be prepared in the long-distance transportation process of the kitchen waste or in the raw material ensilage process, and the volatility of the lactic acid is low, so that the transportation consumption and the possibility of environmental volatilization pollution are reduced.
(2) The loess is improved by using the lactic acid-rich solution to promote plant growth, the soil improvement effect is realized through the evolution of specific type soil rhizosphere microorganisms, the plant growth is promoted, and the plant immunity is improved. The method has reasonable step sequence and has great application prospect. The lactic acid-rich solution is mainly prepared by anaerobic fermentation of biomass wastes such as kitchen waste, so that the cost is low, chemical production in petrochemical industry can be replaced, soil carbon fixation is realized through land utilization, and extremely remarkable carbon reduction benefits are obtained.
Drawings
FIG. 1 is a principal component analysis of wheat rhizosphere bacteria in the treatment and control groups.
FIG. 2 is a principal component analysis of wheat rhizosphere fungi in the treatment and control groups.
Fig. 3 is a principal component analysis of wheat VOCs from the treatment and control groups.
Detailed Description
The preparation and effects of the modified hydrothermal biochar according to the present invention are further described below by way of specific examples. The technical content set forth in these examples is illustrative, but not limiting, and should not be construed as limiting the scope of the invention.
Example 1
Adding different organic acids to yellowImproving soil, wherein the organic acids are acetic acid, propionic acid, butyric acid, lactic acid, citric acid, and malic acid, and the application amount is 12mg-C/cm 2 The same water was added simultaneously as a blank, once every five days. As can be seen from the measurement of the content of the water-soluble total salt in the soil, when lactic acid, citric acid and malic acid are added, the content of the water-soluble total salt in the soil is higher than that of acetic acid, propionic acid and butyric acid, and the effect is best and better than that of acetic acid, propionic acid and butyric acid. Lactic acid is more preferred in view of the cost of lactic acid, malic acid, and citric acid.
The inventors found that the L-lactic acid is highly abundant and less likely to induce a marginal reaction in annual applications because lactic acid in a fermentation broth may have different optical activities such as L-lactic acid, D-lactic acid and DL-lactic acid, and therefore, a lactic acid-rich solution having a high content of L-lactic acid is preferable for improving loess.
Example 2
Obtaining a lactic acid-rich solution: and (3) carrying out anaerobic fermentation on the kitchen waste at 41 ℃ for 3 days, and centrifuging the fermentation liquor for 20 minutes at a rotating speed of 1000rpm to obtain a solution with the L-lactic acid content of 8g/L, the suspended matter content of less than 120mg/L and the pH value of 3.7.
The loess method is improved by the lactic acid-rich solution: diluting the stock solution of the lactic acid-rich solution to 820mg-C/L by tap water after standing overnight, and uniformly irrigating the stock solution of the lactic acid-rich solution into soil; lactic acid was applied in an amount of 0.2 mg-C/g-loess as a treatment group.
The method for cultivating the improved loess comprises the following steps: the loess prepared with the lactic acid rich solution is placed in a greenhouse with the temperature of 25+/-2 ℃ and the humidity of 55+/-7% and is cultivated for 5 days in a dark place. During the cultivation, 50g/kg loess was added as a water amount per day.
Planting plants: wheat was selected as the experimental plant and grown for 33 days. The same volume of deionized water was added as a control group,
loess effect is improved to rich lactic acid solution: mixing loess and water according to water-soil ratio of 5/1, shaking to obtain soil leaching solution, and measuring the contents of soluble Total Carbon (TC), total Organic Carbon (TOC), total Inorganic Carbon (IC) and Total Nitrogen (TN) in the soil leaching solutions of the treatment group and the control group.
The measurement results show that the soluble Total Carbon (TC) content of the treatment group is improved by 16 percent, the Total Organic Carbon (TOC) content is improved by 19.4 percent, and the Total Nitrogen (TN) content is reduced by 44.4 percent compared with the control group.
The wheat stem length and fresh weight were measured, and the results showed that the treated group had 32.5% increase in wheat stem length and 13.6% increase in fresh weight relative to the control group. Therefore, after the lactic acid-rich solution is improved, the organic carbon content in the soil is increased, the nitrogen content is reduced, and the growth of wheat is obviously promoted.
The bacterial and fungal microbial diversity of the microorganisms was confirmed by measuring 16S and 18S rDNA of the rhizosphere microorganisms of the treated group and the control group, and the Principal Component Analysis (PCA) showed that the modified loess was different from the wheat rhizosphere microorganisms in the untreated loess, and as a result, as shown in FIG. 1 (rhizosphere bacteria) and FIG. 2 (rhizosphere fungi), the microorganisms of the treated group and the control group were distributed in different quadrants, and it was seen that the lactic acid solution had an effect on the rhizosphere microorganisms.
Various Volatile Organic Compounds (VOCs) of the overground parts of the wheat in the treatment group and the control group are measured by adopting a gas chromatograph-high-resolution mass spectrometer, and a main component analysis (PCA) shows that the loess after the improvement treatment has obvious difference with the loess without the improvement, and the VOCs in the treatment group and the control group are distributed in different quadrants as shown in a figure 3, so that the lactic acid solution has an influence on the VOCs of the overground parts of the wheat.
While the present invention has been described in detail through the foregoing description of the preferred embodiment, it should be understood that the foregoing description is not to be considered as limiting the invention. Many modifications and substitutions of the present invention will become apparent to those of ordinary skill in the art upon reading the foregoing. Accordingly, the scope of the invention should be limited only by the attached claims.
Claims (9)
1. A method for improving loess to promote plant growth using a lactic acid rich solution, comprising the steps of: (1) loess treatment; (2) preparing a lactic acid-rich solution; (3) improved loess cultivation; wherein the lactic acid-rich solution refers to a solution with the lactic acid content not lower than 1g/L, the proportion of L-lactic acid in lactic acid not lower than 80%, the suspended matter content lower than 2g/L and the pH value between 3.0 and 4.2.
2. The method of claim 1, wherein in the step (3), the lactic acid-rich solution is diluted to 410-4110mg-C/L before being used for the cultivation of the modified loess.
3. The method of claim 2, wherein in the step (3), the amount of lactic acid applied is 0.07-0.75 mg-C/g-loess in terms of lactic acid when the modified loess is cultivated.
4. The method of claim 1, wherein in the step (3), the specific process of the modified loess cultivation is as follows: placing loess prepared with the lactic acid rich solution in a greenhouse with temperature of 25+ -2deg.C and humidity of 55% + -7%, and culturing in dark place for 5-7 days, wherein 28-57g/kg loess is added daily during culturing period to obtain the final product.
5. The method of claim 1, wherein the loess treatment in the step (1) means: firstly, removing animal and plant and gravel residues in a raw loess sample; the soil was then screened and after three days of air drying, all soil samples were placed into the container.
6. The method of claim 5, wherein in step (1), the depth of the container is preferably no more than 40cm; more preferably, each container is filled with soil in an amount of 2/3 to 3/4 of the container volume.
7. The method according to claim 1, wherein in step (2), the lactic acid rich solution is preferably a pretreated raw material derived from biomass waste sources such as kitchen waste fermentation broth, straw silage lixivium, etc.
8. The modified loess prepared by the method as set forth in any one of claims 1 to 7.
9. Use of the modified loess as set forth in claim 8 in plant cultivation.
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202310057275.8A CN116114421A (en) | 2023-01-17 | 2023-01-17 | Method for improving loess by using lactic acid-rich solution to promote plant growth |
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| Application Number | Priority Date | Filing Date | Title |
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| CN202310057275.8A CN116114421A (en) | 2023-01-17 | 2023-01-17 | Method for improving loess by using lactic acid-rich solution to promote plant growth |
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Inventor after: Lv Fan Inventor after: Chen Menmen Inventor after: He Pinjing Inventor after: Zhang Hua Inventor after: Shao Liming Inventor before: Lv Fan Inventor before: Zhang Hua Inventor before: Shao Liming Inventor before: He Pinjing Inventor before: Chen Menmen |
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