CN116103135A - Medical science detects preprocessing device and medical science detection device - Google Patents

Abstract

The invention provides a medical detection pretreatment device and a medical detection device, which belong to the technical field of detection equipment and comprise a nucleic acid extraction unit, a sample adding and distributing unit, a pipetting mechanism and a clamping jaw mechanism; the nucleic acid extraction unit comprises a magnetic rod extraction assembly, a pore plate seat and a first sliding rail; the pipetting mechanism comprises a pipetting gun assembly and a third slide rail; the sample addition and distribution unit comprises a sample rack and a hole plate rack. The device extracts nucleic acid through the magnetic rod extraction component, then carries out sample preparation processing through the sample addition distribution unit, and finally transfers the prepared sample through the pipetting mechanism and the clamping jaw mechanism, so that the whole device is simple and convenient to operate, the automation degree is high, and the detection efficiency is effectively improved.

Description

Medical science detects preprocessing device and medical science detection device

Technical Field

The invention relates to the technical field of detection equipment, in particular to a medical detection pretreatment device and a medical detection device.

Background

Nucleic acid detection is a medical detection mode for in-vitro diagnosis, and can be used for rapidly detecting pathogen nucleic acid in a detection sample. The nucleic acid detection is the most direct, reliable and sensitive method for realizing early, rapid and specific detection of pathogens, and has important application in the fields of disease diagnosis, health monitoring and the like. Nucleic acid detection is performed by detecting genetic materials (such as DNA or RNA) of pathogens to determine information such as the types, the concentrations and the like of the pathogens, and interference of factors such as the disease infection window period and the like is avoided. The detection sample is required to be subjected to nucleic acid extraction treatment before nucleic acid detection, such as nucleic acid extraction involving a plurality of samples, has high requirements on operators and equipment, and the device in the prior nucleic acid extraction pretreatment process is low in automation degree, complex in operation and long in operation time, has high requirements on operators and operation environments, and greatly limits the use population and the use environments of the device.

Therefore, how to make the device capable of simply, conveniently and rapidly detecting and improving the detection efficiency in the pretreatment process of medical detection is a technical problem to be solved in the field.

Disclosure of Invention

Therefore, the technical problem to be solved by the invention is to overcome the defect of low automation degree in the prior art, so as to provide a medical detection pretreatment device and a medical detection device.

In order to solve the problems, a medical detection pretreatment device comprises a nucleic acid extraction unit, a sample adding and distributing unit, a pipetting mechanism and a clamping jaw mechanism;

the nucleic acid extraction unit comprises a magnetic rod extraction assembly, an orifice plate seat and a first sliding rail, wherein the orifice plate seat is arranged on the side face of the first sliding rail at intervals, and the magnetic rod extraction assembly can move along the first sliding rail so as to perform nucleic acid extraction operation on an orifice plate on the orifice plate seat;

the pipetting mechanism comprises a pipetting gun assembly and a third slide rail, and the pipetting gun assembly can reciprocate along the third slide rail;

the sample adding and distributing unit comprises a sample rack and a pore plate rack, the clamping jaw mechanism can clamp and transfer between the sample rack and the pore plate rack, and the clamping jaw mechanism and the pipetting mechanism are both installed on the third slide rail.

Preferably, the pipette assembly has a first slide, the jaw mechanism has a second slide, and the first slide and the second slide are each independently reciprocable along a third slide rail.

Preferably, the clamping jaw mechanism is provided with a first clamping jaw and a second clamping jaw, the first clamping jaw is used for clamping and uncovering a sample tube on the sample rack, and the second clamping jaw is used for clamping and uncovering the pore plate.

Preferably, the first clamping jaw and the second clamping jaw are mounted on two sides of the second sliding arm through the mounting frame.

The sample rack is characterized by further comprising a third clamping jaw and a code scanner, wherein the third clamping jaw is arranged at intervals with the sample rack and used for clamping and fixing the sample tube which is moved out of the sample rack, and the code scanner is arranged on the side face of the sample rack and used for reading the label of the sample tube after clamping and fixing.

Preferably, the orifice plate frame comprises a first tray area, wherein the first tray area comprises at least one deep-hole plate tray, and further comprises a first gun head box, and the first gun head box is arranged adjacent to the deep-hole plate tray and is used for placing a plurality of liquid sucking gun heads.

As a preferable scheme, an auxiliary support is arranged at one side of the first gun head box at intervals, a plurality of gun head holes suitable for placing liquid suction gun heads are formed in the auxiliary support, and the liquid suction gun heads can be extracted from the auxiliary support by the liquid transfer mechanism.

Preferably, the pipetting mechanism is capable of moving a predetermined distance along the length direction of the auxiliary support to extract a corresponding number of pipette tips.

Preferably, the well plate rack further comprises a second tray area, wherein the second tray area comprises at least one shallow well plate tray, and the shallow well plate tray is used for placing shallow well plates for separating liquid.

The liquid sucking gun head is characterized by further comprising a second gun head box which is arranged at intervals with the shallow hole plate tray and used for placing a plurality of liquid sucking gun heads, and the liquid sucking gun heads can be used for sucking liquid and separating liquid.

Preferably, the second tray area further includes at least one deep hole plate tray, and the deep hole plate tray is disposed between the second gun head box and the shallow hole plate tray or adjacent to the second gun head box/shallow hole plate tray.

As a preferred scheme, deep hole board tray includes at least one heating tray, be equipped with the heat conduction seat on the heating tray, the heat conduction seat includes main hot plate and side hot plate, main hot plate interval is provided with the platy protruding of row distribution, side hot plate sets up main hot plate side, and platy protruding extending direction is perpendicular.

Preferably, the gun further comprises a waste groove, wherein the waste groove is arranged between the first gun head box and the second gun head box.

Preferably, the sample adding and distributing unit further comprises a reagent rack, and the reagent rack is arranged between the first tray area and the second tray area.

Preferably, the sample rack is arranged between the nucleic acid extraction unit and the first tray section.

As a preferred scheme, the magnetic rod extraction assembly includes:

a magnetic bar assembly having a plurality of magnetic bars; the magnetic rod assembly is provided with at least two columns;

the magnetic rod sleeve assemblies are provided with the magnetic rod sleeves the same as the magnetic rods in number;

the pore plate seat is provided with at least one pore plate groove for placing a pore plate, and the pore plate is provided with a plurality of rows of reaction holes;

the nucleic acid extraction unit further comprises:

the first moving assembly is provided with a first driving motor and a second driving motor, and the first driving motor drives the magnetic rod assembly to move in the vertical direction; the second driving motor drives the magnetic rod sleeve assembly to move in the vertical direction;

the second moving assembly is provided with a third driving motor, and the third driving motor drives the magnetic rod assembly and the magnetic rod sleeve assembly to move in a horizontal first direction;

and the third moving assembly is provided with a fourth driving motor, the fourth driving motor drives the orifice plate seat to move in a horizontal second direction, and the horizontal second direction is perpendicular to the horizontal first direction.

As a preferred scheme, the magnetic rod extraction assembly further comprises: the second slide rail, the drip-proof plate slides and sets up on the second slide rail, and is located the below of bar magnet cover subassembly.

The device also comprises a film sealing unit, wherein the film sealing unit is arranged adjacent to the sample adding and distributing unit and is used for sealing the shallow pore plate.

Preferably, the film sealing unit includes:

the bottom plate is provided with a bottom plate,

the die frame tray is horizontally arranged in a moving manner relative to the bottom plate, the die frame tray is provided with a mounting hole, a pore plate sealing film is suitable for being placed in the mounting hole, and the pore plate sealing film is in a locking state and an unlocking state in the mounting hole;

the pore plate sealing film comprises a mold frame and a sealing film layer, wherein the mold frame is suitable for being sleeved on the outer surface of the side wall of the pore plate, and the sealing film layer is arranged in the mold frame;

the orifice plate tray is arranged between the die frame tray and the bottom plate and is arranged in a sliding manner relative to the bottom plate; the orifice plate tray has a mounting slot adapted to receive an orifice plate;

the frame pressing mechanism is arranged on the bottom plate and is provided with a frame pressing plate which is arranged in a moving mode in the vertical direction, and the frame pressing plate is used for pressing the pore plate sealing film in the unlocking state onto the pore plate along the film frame.

Preferably, the third slide rail is installed above the nucleic acid extraction unit, the sample adding and distributing unit and the sealing membrane unit, which are sequentially arranged.

Preferably, the first sliding rail and the third sliding rail are mutually perpendicular in the horizontal direction, and the third sliding rail is arranged above the end part of the first sliding rail of the crusher.

The optical detection device further comprises an optical detection unit, a separation plate is arranged between the film sealing unit and the optical detection unit, and a through hole is formed in the separation plate.

The optical detection device also comprises a transmission mechanism, a film sealing unit and a film sealing unit, wherein the transmission mechanism is arranged at the front end of the film sealing unit and is used for transferring a pore plate which is packaged by the film sealing to the optical detection unit through a passing hole on the separation plate;

the transmission mechanism comprises a bearing table, and the bearing table is suitable for placing a sample tray;

the connecting plate is connected with the bearing table;

and the driving motor can drive the connecting plate to move between the film sealing unit and the optical detection unit.

The partition plate further comprises a cover plate, and the cover plate is covered on the through hole.

The technical scheme of the invention has the following advantages:

the invention provides a medical detection pretreatment device, which comprises a nucleic acid extraction unit, a sample adding and distributing unit, a pipetting mechanism and a clamping jaw mechanism, wherein the clamping jaw mechanism is arranged on the sample adding and distributing unit; the nucleic acid extraction unit comprises a magnetic rod extraction assembly, an orifice plate seat and a first sliding rail, wherein the orifice plate seat is arranged on the side face of the first sliding rail at intervals, and the magnetic rod extraction assembly can move along the first sliding rail so as to perform nucleic acid extraction operation on an orifice plate on the orifice plate seat; the pipetting mechanism comprises a pipetting gun assembly and a third slide rail, and the pipetting gun assembly can reciprocate along the third slide rail; the sample adding and distributing unit comprises a sample rack and a pore plate rack, the clamping jaw mechanism can clamp and transfer between the sample rack and the pore plate rack, and the clamping jaw mechanism and the pipetting mechanism are both installed on the third slide rail. The device extracts nucleic acid through the magnetic rod extraction component, then carries out sample preparation processing through the sample addition distribution unit, and finally transfers the prepared sample through the pipetting mechanism and the clamping jaw mechanism, so that the whole device is simple and convenient to operate, the automation degree is high, and the detection efficiency is effectively improved.

Drawings

In order to more clearly illustrate the embodiments of the present invention or the technical solutions in the prior art, the drawings that are needed in the description of the embodiments or the prior art will be briefly described, and it is obvious that the drawings in the description below are some embodiments of the present invention, and other drawings can be obtained according to the drawings without inventive effort for a person skilled in the art.

Fig. 1 is a schematic diagram of the overall structure of a medical detection device according to the present invention.

Fig. 2 is a schematic diagram showing the overall structure of a nucleic acid extraction mechanism of the medical detection device according to the present invention.

Fig. 3 is a schematic structural diagram of a first moving mechanism of the medical detection device provided by the invention.

Fig. 4 is a schematic diagram of switching different pore plates of the medical detection device provided by the invention.

Fig. 5 is a schematic diagram of a sample distribution unit of the medical detection device according to the present invention.

Fig. 6 is a schematic diagram of the overall structure of a heating mechanism of the medical detection device provided by the invention.

Fig. 7 is a schematic structural diagram of a deep hole plate of a heating mechanism of a medical detection device provided by the invention.

Fig. 8 is a cross-sectional view of a deep well plate of a heating mechanism of a medical testing apparatus provided by the present invention.

Fig. 9 is a schematic structural diagram of a deep hole plate of a heating mechanism of a medical detection device provided by the invention.

Fig. 10 is a schematic structural diagram of a heating element of a heating mechanism of a medical detection device according to the present invention.

Fig. 11 is a schematic diagram of the overall structure of a pipetting mechanism of the medical detection device according to the present invention.

Fig. 12 is a schematic view showing a second view of the whole structure of a pipetting mechanism of the medical detection device according to the invention.

Fig. 13 is a schematic structural view of a sixth driving motor of a pipetting mechanism of the medical detection device according to the invention.

Fig. 14 is a schematic structural view of an auxiliary support of a pipetting mechanism of the medical detection device according to the invention.

Fig. 15 is a schematic diagram showing the overall structure of a conveying mechanism of a medical detecting device according to the present invention.

FIG. 16 is a schematic view of a transmission structure of a medical testing apparatus according to the present invention

Fig. 17 is an enlarged view at a in fig. 16.

Fig. 18 is a schematic view of a first angular perspective structure of an orifice sealing film of a medical detection device according to the present invention.

Fig. 19 is a schematic view of a second angular perspective structure of an orifice sealing film of a medical detection device according to the present invention.

Fig. 20 is a schematic perspective view of a film sealing mechanism of a medical detection device according to the present invention.

Fig. 21 is a schematic perspective view of a mold frame tray of a medical detection device according to the present invention.

Fig. 22 is a partial enlarged view at B in fig. 21.

Fig. 23 is a schematic perspective view of an orifice plate tray of the medical detection device according to the present invention.

Fig. 24 is a schematic perspective view of a frame pressing mechanism of a medical detection device according to the present invention.

Fig. 25 is a schematic perspective view of a film pressing mechanism of the medical detection device provided by the invention.

Fig. 26 is a schematic diagram of the mounting structure of the frame tray and the orifice plate tray of the present invention.

Fig. 27 is a schematic structural diagram of a photoelectric correlation switch according to the present invention.

Reference numerals illustrate:

1. a magnetic bar assembly; 2. a magnetic rod sleeve assembly; 3. a pore plate seat; 4. a first driving motor; 5. a second driving motor; 6. a third driving motor; 7. a fourth driving motor; 8. an anti-drip tray; 9. a first conveyor belt; 10. a first slide rail; 11. a second conveyor belt; 12. a second slide rail; 13. a fifth driving motor; 14. a heating element; 15. a heat conduction seat; 16. a protrusion; 17. a deep well plate; 18. a groove; 19. a thermal protection switch; 20. a temperature control sensor; 21. a heat shield; 22. a base; 23. a first gun head box; 24. an auxiliary bracket; 25. a gun head hole; 26. a first slider arm; 27. a third slide rail; 28. a sixth driving motor; 29. a seventh driving motor; 30. an eighth driving motor; 31. a discard tank; 32. a liquid bracket to be tested; 33. a partition plate; 34. a through hole; 35. a cover plate; 36. a carrying platform; 37. a ninth driving motor; 38. a connecting plate; 39. a driving shaft; 40. a driven shaft; 41. a third conveyor belt; 42. a fourth slide rail; 43. a slide block; 44. an upper clamping plate; 45. a lower clamping plate; 46. a second manipulator; 47. a mold frame; 48. sealing film layer; 49. a protective layer; 50. an easy-to-tear handle; 51. a bottom plate; 52. a mold frame tray; 53. a mounting hole; 54. a tongue plate; 55. an elastic member; 56. a guide post; 57. an orifice plate tray; 58. a mounting groove; 59. a frame pressing mechanism; 60. a first mounting bracket; 61. a first top plate; 62. a tenth driving motor; 63. a first moving plate; 64. pressing a frame plate; 65. a rolling wheel; 66. a first lead screw; 67. a first nut; 68. a first optical axis; 69. a film pressing mechanism; 70. a second mounting bracket; 71. a second top plate; 72. an eleventh driving motor; 73. a second lead screw; 74. a second nut; 75. a roller; 76. a second optical axis; 77. a receiving end; 78. a transmitting end; 79. an orifice plate sealing film; 80. a first manipulator; 81. a sample rack; 82. a deep hole plate tray; 83. a shallow hole plate tray; 84. heating the tray; 85. and a reagent rack.

Detailed Description

The following description of the embodiments of the present invention will be made apparent and fully in view of the accompanying drawings, in which some, but not all embodiments of the invention are shown. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.

In the description of the present invention, it should be noted that the directions or positional relationships indicated by the terms "center", "upper", "lower", "left", "right", "vertical", "horizontal", "inner", "outer", etc. are based on the directions or positional relationships shown in the drawings, are merely for convenience of describing the present invention and simplifying the description, and do not indicate or imply that the devices or elements referred to must have a specific orientation, be configured and operated in a specific orientation, and thus should not be construed as limiting the present invention.

In the description of the present invention, it should be noted that, unless explicitly specified and limited otherwise, the terms "mounted," "connected," and "connected" are to be construed broadly, and may be either fixedly connected, detachably connected, or integrally connected, for example; can be mechanically or electrically connected; can be directly connected or indirectly connected through an intermediate medium, and can be communication between two elements. The specific meaning of the above terms in the present invention will be understood in specific cases by those of ordinary skill in the art.

In addition, the technical features of the different embodiments of the present invention described below may be combined with each other as long as they do not collide with each other.

Example 1

As shown in fig. 1 and 2, the medical detection device provided by the invention comprises a nucleic acid extraction unit, a sample adding and distributing unit, a pipetting mechanism, a clamping jaw mechanism, a sealing film unit and an optical detection unit. The third slide rail 27 is installed in nucleic acid extraction unit, application of sample distribution unit and the sealing membrane unit top that sets gradually, and first slide rail 10 and third slide rail 27 are mutually perpendicular in the horizontal direction, and first slide rail 10 is located the front end of nucleic acid extraction unit, and third slide rail 27 sets up in first slide rail 10 tip top.

As shown in fig. 2, the nucleic acid extraction unit in this scheme includes a magnetic rod extraction assembly, a hole plate seat and a first slide rail 10, where the hole plate seat is disposed on a side surface of the first slide rail 10 at intervals, and the magnetic rod extraction assembly can move along the first slide rail 10 to perform a nucleic acid extraction operation on a hole plate on the hole plate seat.

The magnetic bar extraction assembly includes: a magnetic bar assembly 1 having a plurality of magnetic bars, the magnetic bar assembly 1 having at least two columns; the magnetic rod sleeve assembly 2 is provided with magnetic rod sleeves the same as the number of the magnetic rods; the orifice plate seat 3 is provided with at least one orifice plate groove for placing an orifice plate, and the orifice plate is provided with a plurality of rows of reaction holes; the size of the pore plate slot positions can be customized according to the pore plate specification, and the same pore plate can be placed in all the slot positions, or different types of pore plates can be placed in all the slot positions.

The nucleic acid extraction unit further comprises: the first moving assembly is provided with a first driving motor 4 and a second driving motor 5, and the first driving motor 4 drives the magnetic rod assembly 1 to move in the vertical direction; the second driving motor 5 drives the magnetic rod sleeve assembly 2 to move in the vertical direction; the second moving assembly is provided with a third driving motor 6, the third driving motor 6 drives the magnetic rod assembly 1 and the magnetic rod sleeve assembly 2 to move in a horizontal first direction, and the horizontal first direction moves along the extending direction of the orifice plate seat 3 in the scheme; the third moving assembly is provided with a fourth driving motor 7, the fourth driving motor 7 drives the orifice plate seat 3 to move in a horizontal second direction, the horizontal second direction is perpendicular to the horizontal first direction, and the horizontal second direction is perpendicular to the extending direction of the orifice plate seat 3 in the scheme. According to the device, the magnetic rod assembly 1 and the magnetic rod sleeve assembly 2 are moved in the horizontal direction and the vertical direction through the first moving assembly and the second moving assembly, the nucleic acid extraction process of the magnetic rod assembly 1 and the magnetic rod sleeve assembly 2 is guaranteed, when the number of the magnetic rod assemblies 1 is smaller than the number of holes of a pore plate, the magnetic rod assembly 1 only uses single-row holes in the using process, the magnetic rod assembly 1 arranged in the second horizontal direction is defined as a row in the scheme, the magnetic rod assembly arranged in the first horizontal direction is defined as a column, the pore plate seat 3 is driven to move in the horizontal second direction through the third moving assembly, the magnetic rod assembly 1 can use double-row holes, the adaptation between the number of the magnetic rod assemblies 1 and the magnetic rod sleeve assembly 2 and the number of the holes of the pore plate seat 3 are different is realized, and therefore the overall detection efficiency is improved.

The number of the magnetic rods in the device is 48, the magnetic rods are 6*8, the number of the holes of the pore plate is 48 or 96. The device is used for placing a 48-hole plate and a 96-hole plate in the hole plate seat 3 according to capacity requirements. When the 48 pore plates and the 96 pore plates are placed in the pore plate seat, as shown in fig. 4, when the 48 pore plates are used, only 48 magnetic rods are required to correspond to the pore positions of the 48 pore plates, when the 48 pore plates are switched to the 96 pore plates, only the third moving assembly is required to drive the pore plate seat to move the distance of half pore positions in the 48 pore plates in the direction of the vertical pore plate seat 3, at the moment, the magnetic rods just correspond to the singular rows or the double rows in the 96 pore plates, and further, the selection of the singular rows and the double rows in the 96 pore plates is realized by changing the positive and negative directions of the pore plate seat 3 in the vertical pore plate seat. According to the scheme, the 96 pore plates are divided into single lines and double lines, the position of the pore plate seat 3 is changed through the third moving assembly, the 96 pore plates are split into two 48 pore plates for use, and the magnetic bars are switched between the 48 pore plates and the 96 pore plates.

The magnetic bar extraction assembly further comprises: the second slide rail, anti-drip dish 8 slides and sets up on the second slide rail, and is located the below of bar magnet cover subassembly 2, and bar magnet cover subassembly 2 is carrying the nucleic acid transfer in-process at every turn, and the magnetic bead surface is probably carrying too much liquid, and too much liquid can drip under the effect of gravity this moment, and the liquid of drip can be caught by anti-drip dish 8, has avoided polluting other orifice plates. For further increasing the practicality of antidrip dish 8, still be provided with fourth removal subassembly, fourth removal subassembly has fifth driving motor 13, drives antidrip dish 8 through fifth driving motor 13 and removes along the extending direction of orifice plate seat 3 for antidrip dish 8 can follow bar magnet cover subassembly 2 synchronous motion, guarantees bar magnet cover subassembly 2 whole journey and does not pollute other orifice plates, increases the practicality of this device.

The device is provided with a first conveyor belt 9, the first conveyor belt 9 is connected with the output end of a third driving motor 6, the first conveyor belt 9 is fixedly connected with a first moving assembly, the third driving motor 6 is connected with the first moving assembly through the first conveyor belt 9, the first moving assembly is fixedly connected with the conveyor belt and moves along with the movement of the first conveyor belt 9, and the advantages of low noise and low friction of the conveyor belt are utilized to further increase the stability of the first conveyor belt 9. The first sliding rails 10 are provided with two groups, the two groups of first sliding rails 10 are distributed on two sides of the first conveying belt 9, and the first moving assembly is arranged on the first sliding rails 10 in a sliding manner, so that the first moving assembly moves more stably in the horizontal direction.

As shown in fig. 3, the second conveyor belt 11 is connected with the fifth driving motor 13, the second conveyor belt 11 is fixedly connected with the drip-preventing disc 8, the fifth driving motor 13 is connected with the drip-preventing disc 8 through the second conveyor belt 11, the drip-preventing disc 8 is fixedly connected with the second conveyor belt 11 and moves along with the movement of the second conveyor belt 11, and the advantages of low noise and low friction of the conveyor belt are utilized. In order to further increase the stability of the second conveyor belt 11, the second slide rails 12 have two groups, and the two groups of second slide rails 12 are distributed on two sides of the second conveyor belt 11, and the drip tray 8 is slidably arranged on the second slide rails 12, so that the movement of the drip tray 8 in the horizontal direction is more stable.

A sample dispensing unit according to the present invention as shown in fig. 5 includes a sample rack 81 and a well plate rack, and a gripper mechanism capable of performing a gripping transfer operation between the sample rack and the well plate rack, both of which are mounted on a third slide rail 27.

The orifice plate frame includes first tray district and second tray district, and first tray district includes at least one deep hole board tray 82, is provided with first rifle head box 23 adjacent with deep hole board tray 82, and first rifle head box 23 is used for placing a plurality of imbibition rifle heads. The second tray section includes at least one shallow hole plate tray 83, and the shallow hole plate tray 83 houses a shallow hole plate for separating liquid. And a second gun head box which is arranged at intervals with the shallow hole plate tray 83 and is used for placing a plurality of liquid sucking gun heads, wherein the liquid sucking gun heads can be used for sucking and separating liquid. The second pallet section also includes at least one deep-well plate pallet 82, the deep-well plate pallet 82 being disposed between the second gun head box and the shallow-well plate pallet 83 or adjacent to the second gun head box/shallow-well plate pallet 83. The deep hole plate tray 82 is used for placing a deep hole plate, the deep hole plate is used for sample processing and nucleic acid processing, the deep hole plate comprises a 48 deep hole plate and a 96 deep hole plate, the shallow hole plate tray 83 is used for placing a shallow hole plate, the shallow hole plate comprises a 96 shallow hole plate, a 384 shallow hole plate and the like, and the deep hole plate tray can be specifically selected according to detection flux requirements. Further, the deep hole plate tray 82 may be consistent with the specification of the shallow hole plate tray 83, so as to improve the detection efficiency, and the second tray area may also be provided with a deep hole plate for placing the deep hole plate tray to complete nucleic acid extraction, and is adjacent to the shallow hole plate to be separated, so as to facilitate the separation of the liquid by the liquid suction gun head, and after the nucleic acid extraction, the sample in the deep hole plate is separated into the shallow hole plate, so as to facilitate the subsequent film sealing and high-throughput testing.

The deep hole plate tray at least comprises a heating tray 84, a heating mechanism is arranged on the heating tray 84, and the heating mechanism in the device is shown in fig. 6 to 10 and comprises: a heating element 14; the heat conduction seat 15 is connected with the heating element 14, and the heat conduction seat 15 comprises a main heating plate, and a plurality of bulges 16 distributed in rows are arranged on the main heating plate at intervals; the lower surface of the deep hole plate 17 is provided with a groove 18, and the groove 18 is matched with the protrusion 16. Further, the protrusion 16 is a fin-shaped protrusion 16; the device transmits the heat of the heating element 14 to the deep hole plate 17 through the heat conduction seat 15, a plurality of bulges 16 are arranged on the heat conduction seat 15, grooves 18 matched with the bulges 16 are also arranged at the bottom of the deep hole plate 17, and the heat conduction seat 15 heats the grooves 18 in the deep hole plate 17 through the contact of the grooves 18 and the bulges 16, so that the heat conduction seat 15 heats the deep hole plate 17 from the bottom and the inside, the heating is more uniform, the contact area between the heat conduction seat 15 and the deep hole plate 17 is increased by phase change, the heating speed is high, the time is saved, and the efficiency of an analysis process is effectively increased.

As shown in fig. 7, in the present device, the depth of the groove 18 on the lower surface of the deep hole plate 17 also affects the heating speed, but the opening of the groove 18 also occupies the space of the deep hole plate 17, affects the volume in each hole of the deep hole plate 17, and in this embodiment, the depth of the groove 18 is set to be about half the height of the deep hole plate 17, and in order to increase the heating speed of the deep hole plate 17, the groove 18 of the deep hole plate 17 may be lengthened. As shown in fig. 9, in order to facilitate the production and processing and mass production, the protrusions 16 are arranged in rows, and the protrusions 16 in rows/columns can be mass-produced by only a simple process in the processing process, thereby increasing the practicability of the device.

The shape of the bulge 16 is the same as that of the deep hole plate 17, the bulge 16 and the deep hole plate 17 are clamped together through friction force through the cooperation of the bulge 16 and the groove 18, so that the bulge 16 and the deep hole plate 17 are integrally formed, the connection stability of the bulge 16 and the deep hole plate 17 is further ensured, the bulge 16 can be further provided with a buckling position, the buckling is a notch formed in the bulge 16, the groove 18 is internally provided with a joint position matched with the buckling position, each bulge 16 is provided with a plurality of buckling positions, the contact area of the bulge 16 and the deep hole plate 17 is increased through the arrangement of the buckling positions and the joint position, the friction force is increased, and the bulge 16 and the deep hole plate 17 are more firmly connected.

In order to increase the heating efficiency, the projections 16 may be formed in an aligned and combined shape, thereby maximizing the contact area between the projections 16 and the grooves 18 of the deep hole plate 17. Above the deep hole plate 17 are arranged rows/columns of deep holes, with grooves 18 arranged between each row and/or column of deep holes. The shape of the recess 18 matches the shape of the protrusion 16.

Wherein the heat conduction seat 15 further comprises a side heating plate, and the side heating plate is arranged at the side edge of the main heating plate and is perpendicular to the extending direction of the protrusion 16; the side heating plate can assist in heating the hole sites on the side, and heat dissipation of the side holes is avoided.

Optionally, the main heating plate is pre-buried in deep hole board 17 bottom, and main heating plate and deep hole board 17 integrated into one piece can avoid adopting too many heat conductors part to produce heat transfer trouble and produce the influence to the heating result. As an alternative embodiment, the main heating plate may be placed at the bottom of the deep hole plate 17, which can facilitate mass production and increase practicality.

In the practical use process, the deep hole plate 17 generally selects 48 hole plates and 96 hole plates, the side walls of the row deep holes of the 48 hole plates and the 96 hole plates are tightly attached to the side walls of the row deep holes of the 96 hole plates by the aid of the protrusions 16, the distance between every two adjacent plate-shaped protrusions can just accommodate a single row deep hole of the 48 hole plates, and meanwhile, two rows of deep holes of the 96 hole plates can be accommodated, at the moment, only the bottom and one side edge of the single row deep hole of the 96 hole plates are in contact with a main heating plate, but the required heating solution is small in volume, so that the requirement of rapid heating can be met.

As shown in fig. 8-9, in order to further improve the heat conducting performance of the heat conducting seat 15, the material of the heat conducting seat 15 is aluminum, aluminum metal has good heat conducting performance, and the aluminum heat conducting seat 15 has low cost, is convenient for mass production and use, and increases the practicability of the device.

As shown in fig. 10, the device is further provided with a thermal protection switch 19, the thermal protection switch 19 is arranged on one side of the heating element 14, and the thermal protection switch 19 is electrically connected with the heating element 14, when the heating element 14 is abnormal, the heating element 14 is heated to be too high in temperature and easy to be dangerous, the thermal protection switch 19 controls the heating element 14 to stop heating, the whole device is protected, and the safety of the whole device is improved.

The device is also provided with a temperature control sensor 20, the temperature control sensor 20 is arranged on one side of the heating element 14, the temperature control sensor 20 is used for controlling the temperature of the heating element 14, and the temperature of the heating element 14 can be detected and controlled through the temperature control sensor 20 because the temperatures required by different reagents are different, so that the requirements of different reagents are met, and the device has the advantages of accuracy and high efficiency through the control of the temperature control sensor 20, and the practicability of the device is improved.

The device is also provided with a heat shield 21, a heat insulation groove is arranged in the heat shield 21, the heating element 14 is placed in the heat insulation groove, the upper surface of the heating element 14 is directly connected with the heat conduction seat 15, and the heat conduction seat 15 is adaptively connected with the deep hole plate 17; the heat shield 21 is generally made of materials with poor heat conduction performance, the heat shield 21 can effectively isolate heat of the heating element 14 from overflowing, phase change increases the heating speed of the heating element 14, the working efficiency of the whole device is improved, and the practicability of the device is improved.

The device is also provided with a base 22, the heat shield 21 is suitable for being placed on the base 22, the device can be placed on most of work tables through the base 22, and the base 22 is supported on the ground through four legs, so that the whole device is more stable.

The sample addition and dispensing unit further includes a reagent rack 85, the reagent rack 85 being disposed between the first tray section and the second tray section. The sample holder 81 is disposed between the nucleic acid extraction unit and the first tray section.

A pipetting mechanism according to the invention as shown in fig. 11 and 12 comprises a pipetting gun assembly and a third slide 27, the pipetting gun assembly being capable of reciprocating along the third slide 27. The pipetting mechanism includes: the gun head bracket is used for placing a first gun head box 23, and the first gun head box 23 can be used for placing a plurality of liquid suction gun heads in a row; the mounting frame is provided with a pipette assembly in a sliding manner, and the pipette assembly is provided with a plurality of pipette pumps which are arranged at intervals; the auxiliary bracket 24 is arranged at one end of the gun head bracket, and a plurality of gun head holes 25 which are suitable for placing the liquid suction gun heads are arranged on the auxiliary bracket 24; the liquid to be measured bracket 32 is arranged at one side of the gun head bracket and is used for placing liquid to be measured; the driving assembly drives the pipette assembly to move along the direction of the auxiliary bracket 24, and the pipette assembly is moved by a preset distance to obtain a corresponding number of gun heads, in particular to move in the vertical direction, the extending direction of the auxiliary bracket and the moving direction perpendicular to the extending direction of the auxiliary bracket. This device is with liquid-transfering gun subassembly slip setting on the mounting bracket, liquid-transfering gun subassembly removes gun head support top under drive assembly's drive, and obtain the liquid-transfering gun head of a whole row on the gun head support under drive assembly's drive, later remove liquid-transfering gun subassembly that has the liquid-transfering gun head to auxiliary stand 24 top, with a whole row liquid-transfering gun head, all place on auxiliary stand 24, later adjust the relative position of liquid-transfering gun subassembly and auxiliary stand 24 through drive assembly, make the liquid-transfering gun subassembly can select the liquid-transfering gun head of part on the auxiliary stand 24, make the quantity of gun head quantity on the liquid-transfering gun subassembly can adapt to the quantity of test liquid, can not cause the wasting of resources, the efficiency of whole process has been improved.

When a single sample tube needs to be operated or 8 gun heads do not need to be used for pipetting at a time, the gun head holes 25 in the auxiliary support 24 are 8, and when the single sample tube needs to be operated or 8 gun heads do not need to be used for pipetting at a time, the gun head holes 25 in the auxiliary support 24 are eight, and the longitudinal spacing of the disposable gun heads in the first gun head box 23 is 9mm, and the spacing of the gun head holes 25 is also 9mm, so that the auxiliary support 24 can be better matched with the use of the first gun head box 23 because the first gun head box 23 is generally arranged in 96 rows and 12 columns. Under the condition that an auxiliary support is not needed, a sample to be tested (generally solid) can be directly placed on the deep hole plate after being processed, and then 8 pipette assemblies are operated at a time for processing.

The mounting bracket in this device is including first wiper 26 and second wiper, with the one end sliding connection of pipette subassembly and first wiper 26, clamping jaw mechanism sets up on the second wiper, first clamping jaw and second clamping jaw are all installed in the both sides of second wiper through the mounting bracket, first wiper 26, second wiper and third slide rail 27 sliding connection, homoenergetic independently follow third slide rail 27 reciprocating motion to second wiper and first wiper 26 parallel arrangement. And the first sliding arm 26 and the second sliding arm are both arranged in the horizontal direction, so that the pipette assembly can move randomly in the horizontal direction under the action of the first sliding arm 26 and the second sliding arm.

The clamping jaw mechanism comprises a first mechanical arm 80, a first clamping jaw and a second clamping jaw are simultaneously installed on the first mechanical arm 80, the first clamping jaw clamps and uncovers the sample tube, the second clamping jaw is used for clamping the pore plate, the first clamping jaw and the second clamping jaw transfer the pore plate from the nucleic acid extraction unit to the first tray area and the second tray area of the sample adding and distributing unit, the pore plate of the first tray area and the pore plate of the second tray area transfer the pore plate to the membrane sealing unit to conduct membrane sealing operation and the like. The first clamping jaw clamps on the cover of the sample tube, the cover is rotationally taken down through the rotating motor, the sample tube further comprises a third clamping jaw and a code scanner, the third clamping jaw is arranged at intervals with the sample frame and used for clamping and fixing the sample tube, the code scanner is arranged on the side face of the sample frame and used for reading a label of the sample tube after clamping and fixing, and the working states of the first clamping jaw, the second clamping jaw and the third clamping jaw are selected according to requirements.

As shown in fig. 12, in order to secure a driving force of the movement of the pipette assembly, the driving assembly includes a first driving assembly, a second driving assembly, and a third driving assembly; the first driving assembly comprises a sixth driving motor 28, the output end of the sixth driving motor 28 is connected with the pipette assembly, and the sixth driving motor 28 drives the pipette assembly to move in the vertical direction, wherein the vertical direction in the scheme is the movement in the vertical direction; in order to realize the connection between the sixth driving motor 28 and the pipette assembly, a screw structure is arranged between the sixth driving motor 28 and the pipette assembly, and the rotation motion of the output shaft of the sixth driving motor 28 is converted into linear motion through the screw structure, and meanwhile, the characteristics of high precision, reversibility and high efficiency are achieved.

As shown in fig. 13, a retracting mechanism is further provided on one side of the sixth driving motor 28, the retracting mechanism including a retracting motor and a retracting head; the motor that moves back sets up on the liquid-transfering gun subassembly, the output of motor that moves back is connected with moving back the head, move back the headgear and establish on the rifle head of liquid-transfering gun subassembly, because the liquid-transfering gun carries out the butt with the imbibition rifle head through sixth driving motor 28, be connected liquid-transfering rifle head and liquid-transfering gun subassembly rifle head through frictional force, so can slide on the liquid-transfering rifle head through moving back the motor area that moves back the head, can move back the imbibition rifle head after liquid-transfering rifle head uses to accomplish, increase whole device's degree of automation, whole device is more efficient.

The second driving assembly comprises a seventh driving motor 29, the seventh driving motor 29 is arranged on the first sliding arm 26, the seventh driving motor 29 is arranged at one end of the first sliding arm 26 close to the second sliding arm and drives the pipette assembly to move along the extending direction of the auxiliary bracket 24, the seventh driving motor 29 is combined with the sliding rail structure through a servo motor, and movement of the pipette assembly in the extending direction of the auxiliary bracket 24 is achieved.

The third driving assembly comprises an eighth driving motor 30 and a controller, the eighth driving motor 30 is arranged on the third sliding rail 27, the eighth driving motor 30 is arranged at one end of the third sliding rail 27 close to the first sliding arm 26, the liquid-transferring gun assembly is driven to move vertically to the upper side of the first gun head box 23 under the combined action of the eighth driving motor 30 and the seventh driving motor 29, the eighth driving motor 30 is electrically connected with the controller, the combination of the liquid-transferring gun assembly and the sliding rail structure is realized, the movement of the liquid-transferring gun assembly in the moving direction vertical to the extending direction of the auxiliary bracket 24 is realized, the controller is used for controlling the eighth driving motor 30, the driving distance of the eighth driving motor 30 can be controlled, when the liquid-transferring gun assembly needs a plurality of gun heads (the quantity is smaller than 8), the liquid-transferring gun assembly is moved to the upper side of the first gun head box 23 under the combined action of the eighth driving motor 29, the liquid-transferring gun assembly is regulated to move vertically, the liquid-transferring gun assembly is enabled to take a row of liquid-transferring gun heads from the first gun head box 23 to the auxiliary bracket 24, and then the liquid-transferring gun assembly is enabled to be placed to the position above the auxiliary bracket through the liquid-transferring gun heads 25, and the liquid-transferring gun assembly can be completely required to be controlled, and the liquid-transferring gun heads can be prevented from being required to be completely and the liquid-transferring gun assembly is required to be placed on the auxiliary bracket through the auxiliary bracket. In the device, 8-row pipettors are selected as the pipette assembly.

When 8 gun heads are used once, the pipette gun assembly directly moves to the position above the first gun head box 23, moves downwards, sleeves 8 pipette gun heads, and then performs pipetting.

When 1 tip is required for a single use, the pipette gun assembly is moved above the first tip box 23, moved downward, and the 8 pipette tips are pulled over the auxiliary support 24, and the 8 pipette tips are pulled down to drop into the tip holes 25 of the auxiliary support 24, as shown in fig. 14. The pipette gun assembly is longitudinally moved by 63mm, so that the innermost pipette gun assembly is aligned with the outermost pipette gun head (the pipette gun assembly can be reversed), and then the pipette gun heads are sleeved downwards, so that only one pipette gun head is installed on the pipette gun assembly, and the rest positions are empty. The first pipette tip is discarded after use, the longitudinal offset distance is 54mm when the pipette tip is fetched for the second time, and so on, and finally the selected pipette tip assembly is moved to the position above the liquid to be measured bracket 32 to extract the liquid to be measured.

The device is also provided with a waste groove 31, the waste groove 31 is arranged between the first gun head box 23 and the second gun head box, after the liquid to be tested is tested by the liquid-transferring gun assembly, the liquid-transferring gun assembly is moved to the upper part of the waste groove 31, the liquid-transferring gun heads are retracted and fall into the waste groove 31, the collection of the waste gun heads is realized, and the efficiency and the practicability of the device are improved.

The device also comprises a film sealing unit for sealing the pore plate, and the film sealing unit is arranged adjacent to the second tray area.

As shown in fig. 18 to 19, the film sealing mechanism provided by the invention comprises a mold frame 47, a film sealing layer 48 and a protective layer 49; the mold frame 47 is rectangular and is matched with the size of the pore plate, and is suitable for being sleeved on the outer surface of the side wall of the pore plate. The inside of the mold frame 47 is smooth, has a slope, and is more conveniently sleeved on the side wall of the orifice plate.

A sealing film layer 48 is adhered to the upper surface of the mold frame 47, the sealing film layer 48 has an adhesive surface, the adhesive surface is adhered to the mold frame 47, and the adhesive surface is used for adhering to the orifice plate. The adhesive surface is covered with a protective layer 49, and the protective layer 49 is used for protecting the adhesive surface from contamination. One end of a tearing handle 50 is connected to the protective layer 49, and the other end of the tearing handle 50 extends in a direction away from the protective layer 49.

This orifice plate sealing membrane 79 is through setting up the mould frame 47, makes things convenient for people to take, does not need operating personnel to directly take sealing membrane layer 48, and then can not cause the pollution, has the protection of protective layer 49 at ordinary times simultaneously, has reduced unnecessary pollution.

As shown in fig. 20, further comprising: a base plate 51, a die frame tray 52, an orifice plate tray 57, a frame pressing mechanism 59, and a film pressing mechanism 69.

As shown in fig. 21, the mold frame tray 52 is used to mount and transfer the orifice plate sealing film 79 described above. Specifically, the mold frame tray 52 is slidably disposed on the bottom plate 51, the mold frame tray 52 has a mounting hole 53 for placing the orifice plate sealing film 79, the size of the mounting hole 53 is identical to the size of the mold frame 47, and the orifice plate sealing film 79 has a locked state and an unlocked state in the mounting hole 53.

A pore plate sealing film limiting piece is arranged on the mold frame tray, and can extend into the mounting hole; the orifice plate sealing film limiting piece can extend into the orifice plate sealing film limiting piece in an axial rotation mode or a sliding mode.

As shown in fig. 22, through holes are formed on both sides of the mounting hole 53 of the mold frame tray 52, and a lock mechanism is provided in the through holes. Specifically, one end of the tongue plate 54 is installed in the through hole, and the other end of the tongue plate 54 protrudes toward the installation hole 53; an elastic member 55 is provided between the tongue plate 54 and the inside of the through hole, the elastic member 55 having a driving force that drives the tongue plate 54 to protrude toward the mounting hole 53. Meanwhile, a guide post 56 is vertically disposed on the tongue plate 54 located in the through hole, and further, two guide posts 56 are disposed on one tongue plate 54 plate.

A guide rail and a sliding block 43 are installed below the mold frame tray 52, the sliding block 43 is connected with a screw nut, the screw nut is connected with a motor through a screw, and the motor is fixedly arranged on the bottom plate 51. The motor drives the orifice plate tray 57 to move on the chassis.

In use, under the action of the resilient member 55, the tongue plate 54 extends out of the mounting hole 53 and holds the orifice plate sealing membrane 79 so that the orifice plate sealing membrane 79 does not fall. Then, by pushing the guide post 56 against the driving force of the elastic member 55, the tongue plate 54 is retracted to the direction of the through hole, and the orifice plate sealing film 79 is not pulled any more, so that the orifice plate sealing film 79 can be dropped from the mounting hole 53.

As shown in fig. 23, the well plate tray 57 is used to mount and push well plates, in this case 384 well plates. The orifice plate tray 57 is provided with a mounting groove 58 for placing the orifice plate, and a plurality of clamping plates and clamping positions are arranged in the mounting groove 58 for fixing the orifice plate.

Two guide rails are mounted on the bottom plate 51, and the orifice plate tray 57 is slidably provided on the guide rails by the slider 43. A screw nut is arranged at the center of the bottom of the tray, and the screw nut is connected with a motor through a screw.

As shown in fig. 24, the frame pressing mechanism 59 functions to press the orifice plate sealing film 79 down onto the orifice plate. The frame pressing mechanism 59 includes a first mounting bracket 60, the first mounting bracket 60 is mounted on the bottom plate 51, a first top plate 61 is provided at an upper end of the first mounting bracket 60, a tenth driving motor 62 is mounted on the first top plate 61, and further, the tenth driving motor 62 is a stepping motor.

The tenth driving motor 62 is connected with a first moving plate 63 through a first transmission assembly, a frame pressing plate 64 is installed at the lower end of the first moving plate 63, and rolling wheels 65 are provided at both sides of the frame pressing plate 64. Further, the tenth driving motor 62 is connected to the upper end of the first screw 66 through a timing belt, and the lower end of the first screw 66 is rotatably connected to the bottom plate 51 through a bearing. The first screw rod 66 is sleeved with a first nut 67, and the first nut 67 is connected with the first moving plate 63. The tenth driving motor 62 is realized to drive the frame pressing plate 64 to move.

In order to further ensure the stability of the movement of the first nut 67, a first optical axis 68 is provided at one side of the first screw, and the first nut 67 is connected to the first optical axis 68 through a linear bearing.

In the use process, when the orifice plate tray 57 and the frame film tray are arranged on the first moving plate 63, the tenth driving motor 62 drives the first moving plate 63 to move downwards, the rolling wheel 65 firstly pushes the guide post 56 to retract the tongue plate 54, the orifice plate sealing film 79 is in an unlocking state and moves downwards continuously, and the frame pressing plate 64 presses the die frame 47 of the orifice plate sealing film 79 to enable the die frame to be buckled above the orifice plate.

As shown in fig. 25, the film pressing mechanism 69 is used to provide a pressing force to adhere the seal film 48 to the surface of the orifice plate. Specifically, a second mounting bracket 70 is mounted on the bottom plate 51, a second top plate 71 is provided at an upper end of the second mounting bracket 70, an eleventh driving motor 72 is mounted on the second top plate 71, and the eleventh driving motor 72 is connected to the roller 75 through a second transmission assembly.

The eleventh driving motor 72 is connected to the upper end of the second screw 73 through a timing belt, and the lower end of the second screw 73 is rotatably connected to the bottom plate 51 through a bearing. The second screw 73 is sleeved with a second nut 74, and the first nut 67 is connected with a roller 75. The eleventh driving motor 72 is realized to drive the movement of the roller 75.

As shown in fig. 26, in order to further secure the smoothness of the movement of the second nut 74, a second optical axis 76 is provided at one side of the second screw, and the first nut 67 is connected to the second optical axis 76 through a linear bearing.

Springs are provided on both sides of the roller 75 for ensuring a quantitatively controllable pressing force.

As shown in fig. 27, in order to detect whether the orifice sealing film 79 and the orifice are properly mounted, a photo-correlation switch is provided, which has a receiving end 77 and a transmitting end 78 disposed opposite to each other, the transmitting end 78 being disposed on the bottom plate 51, and the receiving end 77 being disposed on the second top plate 71.

A separation plate is arranged between the film sealing unit and the optical detection unit. The front end of the film sealing unit is provided with a transmission mechanism for transferring the pore plate which is packaged by the film sealing to the optical detection unit through the passing hole on the partition plate.

As shown in fig. 15 to 17, the optical detection device further comprises a transmission mechanism movably arranged between the film sealing unit and the optical detection unit; the partition plate 33 is arranged between the film sealing unit and the optical detection unit in a blocking manner, a through hole 34 is formed in the partition plate 33, and the through hole 34 is in an open state when the transmission mechanism passes through and is also in a closed state when the transmission mechanism does not pass through. Placing the pore plate on a transmission mechanism, and moving the pore plate between the film sealing unit and the optical detection unit under the drive of the transmission mechanism; the orifice plate passes through the through holes 34 on the partition plate 33 under the drive of the transmission mechanism, the through holes 34 are in an open state when the orifice plate passes through, and are in a closed state when the orifice plate does not pass through, so that independent sealing of different working areas can be effectively avoided, manual carrying is effectively avoided, and the degree of automation is high;

In order to ensure the tightness between the film sealing unit and the optical detection unit, a cover plate 35 is further arranged, the cover plate 35 is covered on the through hole 34, the cover plate 35 is rotatably arranged on the partition plate 33, and when the through hole 34 is in an open state, the cover plate 35 rotates under the action of external force to open the through hole 34; when the passing hole 34 is in a closed state, the cover plate 35 is in a stationary state and covers the passing hole 34 to close the passing hole 34; the cover plate 35 can further isolate the film sealing unit from the optical detection unit, so that mutual infection is avoided, the cover plate 35 is connected with the partition plate 33 through a hinge, the cover plate 35 is arranged on the through hole 34, the cover plate 35 is connected with a spring, the spring is arranged on the hinge and provides driving force for the cover of the cover plate 35, and the cover is arranged on the partition plate 33 when the cover plate 35 is static and can be opened only under the action of external force. In this embodiment, the transmission mechanism is placed in the film sealing unit, the cover plate 35 is disposed in the optical detection unit, and the transmission mechanism and the cover plate 35 are not simultaneously present in one area, or the transmission mechanism may be placed in the optical detection unit, and the cover plate 35 may be placed in the film sealing unit.

As shown in fig. 16, the transmission mechanism in the present apparatus further includes: a carrying table 36 and a ninth driving motor 37, the carrying table 36 is suitable for placing the hole plate, the carrying table 36 is also suitable for penetrating through the through hole 34, the section of the carrying table 36 is smaller than the size of the through hole 34, the carrying table 36 is connected with the ninth driving motor 37, the ninth driving motor 37 drives the carrying table 36 to reciprocate, and the ninth driving motor 37 is a power source of the whole conveying device. The transmission mechanism is further provided with a connecting plate 38, the connecting plate 38 is L-shaped, the long side of the connecting plate 38 is fixedly connected with the bottom of the bearing table 36, the short side of the connecting plate 38 is connected with the ninth driving motor 37, the connecting plate 38 connects the ninth driving motor 37 and the bearing table 36 into a whole, the device has the advantages of simplicity and high efficiency, and the practicality of the device is improved.

As shown in fig. 17, a slider 43 is further provided on the short side of the connection plate 38, and the slider 43 is slidably provided on the fourth slide rail 42 so that the connection plate 38 moves on the fourth slide rail 42 together with the slider 43. Fourth slide rail 42 and third conveying 41 parallel arrangement to set up the top at third conveyer belt 41 with fourth slide rail 42, set up like this and can improve the stability of connecting plate 38 when removing, guarantee the steady operation of whole device, increase the practicality of this device.

The output shaft of the ninth driving motor 37 is fixedly connected with the driving shaft 39, the driven shaft 40 is further arranged at a position corresponding to the driving shaft 39, the driving shaft 39 and the driven shaft 40 are arranged in parallel, the driving shaft 39 is provided with the third conveying belt 41, the third conveying belt 41 is sleeved on the driving shaft 39 and the driven shaft 40, the ninth driving motor 37 drives the third conveying belt 41 to operate, the connecting plate 38 is connected with the third conveying belt 41, the movement that the ninth driving motor 37 drives the connecting plate 38 is indirectly realized, the indirect transmission through the third conveying belt 41 has the advantage of high transmission ratio, and the device is simple in structure, easy to operate and high in practicability. The rotation of the ninth driving motor 37 may be changed to horizontal movement not only by using the third conveyor belt 41 but also by a screw structure.

The third conveyer belt 41 is connected with the connecting plate 38 in this device, wherein be provided with the holder on the third conveyer belt 41, the holder centre gripping is on the third conveyer belt 41, the holder includes punch holder 44 and lower plate 45, punch holder 44 and slider 43 fixed connection, lower plate 45 passes through the bolt and is connected with punch holder 44, and can adjust the clearance size between punch holder 44 and the lower plate 45 through the bolt, set up the advantage that has the regulation of being convenient for like this, be provided with the flank of tooth at the upper surface of lower plate 45, the flank of tooth can increase the pressure of lower plate 45 to the third conveyer belt 41, make the holder more firm at the centre gripping in-process, can not take place the relative slip, increase the transmission ratio, increase the practicality of this device.

The device is also provided with a second manipulator 46 at the optical detection unit, the second manipulator 46 is used for grabbing the pore plate penetrating through the through hole 34, the automation degree of the whole device is further increased, the manual workload is effectively reduced, the working efficiency is increased, the second manipulator 46 is connected with a servo motor in the scheme, and the second manipulator 46 is driven to move in the vertical direction through a private motor, so that the second manipulator 46 can take the pore plate off the bearing table 36 after clamping the pore plate.

Example 2

The specific steps of the detection using the medical detection pretreatment apparatus of embodiment 1 include:

(1) The clamping jaw mechanism is used for clamping and uncovering the sample tube;

(2) The liquid in the sample tube is transferred into a 48-hole plate of the sample adding and distributing unit by the liquid transferring mechanism;

(3) Heating the pore plate by a heating tray in the first tray area after adding the corresponding reagent;

(4) The jaw mechanism transfers the 48-well plate to the nucleic acid extraction unit;

(5) The nucleic acid extraction unit performs purification operation by a magnetic bead method;

(6) The liquid transferring structure divides the purified sample liquid into 384-hole plates and adds the reaction liquid;

(7) The clamping jaw mechanism transfers the 384-hole plate to a film sealing unit, and the film sealing unit withdraws after film sealing operation;

(8) The clamping jaw mechanism transfers the 384-hole plate after film sealing to the transmission mechanism and conveys the 384-hole plate to the optical detection unit;

(9) The optical detection unit performs amplification detection and outputs a detection result.

It is apparent that the above examples are given by way of illustration only and are not limiting of the embodiments. Other variations or modifications of the above teachings will be apparent to those of ordinary skill in the art. It is not necessary here nor is it exhaustive of all embodiments. While still being apparent from variations or modifications that may be made by those skilled in the art are within the scope of the invention.

Claims (23)

1. The medical detection pretreatment device is characterized by comprising a nucleic acid extraction unit, a sample adding and distributing unit, a pipetting mechanism and a clamping jaw mechanism;

the nucleic acid extraction unit comprises a magnetic rod extraction assembly, an orifice plate seat (3) and a first sliding rail (10), wherein the orifice plate seat (3) is arranged on the side face of the first sliding rail (10) at intervals, and the magnetic rod extraction assembly can move along the first sliding rail (10) so as to perform nucleic acid extraction operation on an orifice plate on the orifice plate seat (3);

the pipetting mechanism comprises a pipetting gun assembly and a third slide rail (27), the pipetting gun assembly being capable of reciprocating along the third slide rail (27);

the sample adding and distributing unit comprises a sample rack and a pore plate rack, the clamping jaw mechanism can clamp and transfer between the sample rack and the pore plate rack, and the clamping jaw mechanism and the pipetting mechanism are both installed on a third sliding rail (27).

2. Medical detection pretreatment device according to claim 1, characterized in that the pipette assembly has a first slide (26), the gripper mechanism has a second slide, and the first slide (26) and the second slide are each independently reciprocable along a third slide rail (27).

3. The medical testing pretreatment apparatus according to claim 2, wherein the gripper mechanism has a first gripper for gripping and uncapping the sample tube on the sample rack and a second gripper for gripping the well plate.

4. A medical testing pretreatment device according to claim 3, wherein the first jaw and the second jaw are mounted on both sides of the second slide arm by means of a mounting bracket.

5. A medical testing pretreatment device according to claim 3, further comprising a third clamping jaw and a code scanner, wherein the third clamping jaw is arranged at intervals with the sample rack and is used for clamping and fixing the sample tube removed from the sample rack, and the code scanner is arranged on the side surface of the sample rack and is used for reading the label of the sample tube after clamping and fixing.

6. The medical detection pretreatment apparatus according to any one of claims 1 to 5, wherein the well plate rack comprises a first tray section comprising at least one deep-well plate tray, and further comprising a first gun head box (23) disposed adjacent to the deep-well plate tray for placing a plurality of pipette tips.

7. Medical detection pretreatment device according to claim 6, characterized in that an auxiliary support (24) is arranged at one side of the first gun head box (23) at intervals, a plurality of gun head holes (25) suitable for placing liquid suction gun heads are arranged on the auxiliary support (24), and the liquid transfer mechanism can extract at least one liquid suction gun head from the auxiliary support (24).

8. The medical detection pretreatment apparatus according to claim 7, wherein the pipetting mechanism is movable a predetermined distance along a length direction of the auxiliary stand (24) to extract a corresponding number of the pipette tips.

9. The medical detection pretreatment apparatus according to claim 6, wherein the orifice plate carrier further comprises a second tray section including at least one shallow orifice plate tray (83), the shallow orifice plate tray (83) placing a shallow orifice plate for liquid separation.

10. The medical testing pretreatment apparatus of claim 9, further comprising a second tip box spaced from said shallow plate tray (83) for holding a plurality of pipette tips for pipetting and dispensing.

11. The medical detection pretreatment apparatus according to claim 10, wherein the second tray section further comprises at least one deep-well plate tray provided between the second gun head box and the shallow-well plate tray (83) or provided adjacent to the second gun head box/the shallow-well plate tray (83).

12. Medical detection pretreatment device according to claim 11, characterized in that the deep-hole plate tray comprises at least one heating tray, the heating tray is provided with a heat conducting seat (15), the heat conducting seat (15) comprises a main heating plate and side heating plates, the main heating plates are provided with plate-shaped bulges (16) distributed in rows at intervals, and the side heating plates are arranged on the side edges of the main heating plates and are perpendicular to the extending direction of the plate-shaped bulges (16).

13. The medical detection pretreatment apparatus according to claim 10, further comprising a discard tank (31), the discard tank (31) being disposed between the first gun head box (23) and the second gun head box.

14. The medical testing pretreatment device of claim 9, wherein the sample addition and dispensing unit further comprises a reagent rack disposed between the first tray section and the second tray section.

15. The medical detection pretreatment device according to claim 6, wherein the sample rack is disposed between the nucleic acid extraction unit and the first tray section.

16. The medical detection pretreatment apparatus according to claim 1, wherein the magnetic rod extraction assembly comprises:

A magnetic rod assembly (1) having a plurality of magnetic rods; the magnetic rod assembly (1) is provided with at least two rows;

the magnetic rod sleeve assembly (2) is provided with magnetic rod sleeves the same as the magnetic rods in number;

the pore plate seat (3) is provided with at least one pore plate groove for placing a pore plate, and the pore plate is provided with a plurality of rows of reaction holes;

the nucleic acid extraction unit further comprises:

the first moving assembly is provided with a first driving motor (4) and a second driving motor (5), and the first driving motor (4) drives the magnetic rod assembly (1) to move in the vertical direction; the second driving motor (5) drives the magnetic rod sleeve assembly (2) to move in the vertical direction;

the second moving assembly is provided with a third driving motor (6), and the third driving motor (6) drives the magnetic rod assembly (1) and the magnetic rod sleeve assembly (2) to move in a horizontal first direction;

and the third moving assembly is provided with a fourth driving motor (7), and the fourth driving motor (7) drives the orifice plate seat (3) to move in a horizontal second direction which is perpendicular to the horizontal first direction.

17. The medical detection pre-processing apparatus according to claim 16, wherein the magnetic rod extraction assembly further comprises: the second sliding rail (12) and the drip-proof disc (8) are arranged on the second sliding rail (12) in a sliding manner and are positioned below the magnetic rod sleeve assembly (2).

18. The medical testing pretreatment device of any of claims 9 to 14, further comprising a sealing unit disposed adjacent to the loading dispensing unit for sealing the shallow well plate.

19. The medical detection pretreatment apparatus according to claim 18, wherein the film sealing unit comprises:

a bottom plate (51),

a mold frame tray (52) horizontally movably arranged relative to the bottom plate (51), wherein the mold frame tray is provided with a mounting hole (53), an orifice plate sealing film (79) is suitable for being placed in the mounting hole (53), and the orifice plate sealing film (79) is in a locking state and an unlocking state in the mounting hole (53);

the orifice plate sealing film (79) comprises a die frame (47) which is suitable for being sleeved on the outer surface of the side wall of the orifice plate and a sealing film layer (48) which is arranged in the die frame (47);

an orifice plate tray (57) provided between the mold frame tray (52) and the bottom plate (51), and slidably provided with respect to the bottom plate (51); the orifice plate tray (57) has mounting slots (58) adapted to receive an orifice plate;

the frame pressing mechanism (59) is arranged on the bottom plate (51), the frame pressing mechanism (59) is provided with a frame pressing plate (64) which is arranged in a moving mode in the vertical direction, and the frame pressing plate (64) is used for pressing the orifice plate sealing film (79) in the unlocking state onto the orifice plate along the film frame (47).

20. The medical detection pretreatment device according to claim 18, wherein the third slide rail (27) is installed above the nucleic acid extraction unit, the sample addition and distribution unit, and the sealing membrane unit, which are sequentially provided.

21. The medical detection pretreatment apparatus according to claim 20, characterized in that the first slide rail (10) and the third slide rail (27) are mutually perpendicular in the horizontal direction, and the third slide rail (27) is disposed above the end of the first slide rail (10).

22. A medical testing device, characterized by comprising the medical testing pretreatment device according to any one of claims 18-21, and further comprising an optical testing unit, wherein a separation plate is arranged between the sealing film unit and the optical testing unit, and a through hole (34) is arranged on the separation plate.

23. The medical detection device according to claim 22, further comprising a transmission mechanism arranged at the front end of the film sealing unit for transferring the aperture plate completed with film sealing encapsulation to the optical detection unit through a through hole (34) on the partition plate;

the transmission mechanism comprises a bearing table, and the bearing table is suitable for placing a sample tray;

a connection plate (38) connected to the carrying floor;

A second drive motor (5), the second drive motor (5) being capable of driving the connection plate (38) to move between the film sealing unit and the optical detection unit;

the partition plate further comprises a cover plate (35), and the cover plate (35) is arranged on the through hole (34) in a covering mode.

Images