CN116077400A - Plant formula with antioxidant and anti-aging effects and application thereof - Google Patents
Plant formula with antioxidant and anti-aging effects and application thereof Download PDFInfo
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Images
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9789—Magnoliopsida [dicotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9728—Fungi, e.g. yeasts
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K8/00—Cosmetics or similar toiletry preparations
- A61K8/18—Cosmetics or similar toiletry preparations characterised by the composition
- A61K8/96—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution
- A61K8/97—Cosmetics or similar toiletry preparations characterised by the composition containing materials, or derivatives thereof of undetermined constitution from algae, fungi, lichens or plants; from derivatives thereof
- A61K8/9783—Angiosperms [Magnoliophyta]
- A61K8/9794—Liliopsida [monocotyledons]
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61Q—SPECIFIC USE OF COSMETICS OR SIMILAR TOILETRY PREPARATIONS
- A61Q19/00—Preparations for care of the skin
- A61Q19/08—Anti-ageing preparations
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K2800/00—Properties of cosmetic compositions or active ingredients thereof or formulation aids used therein and process related aspects
- A61K2800/40—Chemical, physico-chemical or functional or structural properties of particular ingredients
- A61K2800/59—Mixtures
- A61K2800/592—Mixtures of compounds complementing their respective functions
- A61K2800/5922—At least two compounds being classified in the same subclass of A61K8/18
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Veterinary Medicine (AREA)
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- Animal Behavior & Ethology (AREA)
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- Botany (AREA)
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- Engineering & Computer Science (AREA)
- Dermatology (AREA)
- Gerontology & Geriatric Medicine (AREA)
- Medicines Containing Plant Substances (AREA)
Abstract
The invention discloses a plant formula with antioxidant and anti-aging effects, which comprises a functional substance, deionized water and glycerin in a weight ratio of 7:70:30, wherein the functional substance comprises 15 parts of monarch drug, 67.5 parts of ministerial drug, 6 parts of adjuvant drug and 15 parts of conductant drug in parts by weight, the monarch drug comprises kylin, the ministerial drug comprises safflower, red paeony root, angelica tail, ginseng, poria cocos and bighead atractylodes rhizome, the adjuvant drug comprises dried ginger, cinnamon and clove, and the conductant drug is liquorice, and the plant formula can be applied to the preparation of skin care products and cosmetics. The plant formula of the invention has mild effect, has the effects of resisting wrinkles and aging, and has double effects of nutrition and curative effect.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a plant formula with antioxidant and anti-aging effects and application thereof.
Background
With the development of economy and the continuous improvement of living standard, people have an increasing attention to skin health and maintenance. The skin care product can add a layer of protective barrier to the skin of people, reduce the damage of the skin to external physical and chemical factors, delay the generation of skin wrinkles and slow down aging. From the aspect of anti-wrinkle raw materials, currently generally used anti-wrinkle raw materials comprise enzymes, peptides, vitamins, plant active ingredient categories and the like, but with the continuous improvement of the requirements of people on the safety and environmental protection of skin care products, an anti-wrinkle anti-aging raw material with higher economical safety, environmental protection and reliable curative effect needs to be sought.
Disclosure of Invention
In order to solve the problems, the invention provides a plant formula with antioxidant and anti-aging effects and application thereof.
The invention adopts the following technical scheme:
the plant formula with the effects of resisting oxidization and aging comprises the functional substances, deionized water and glycerin in a weight ratio of 7:70:30, wherein the functional substances comprise 15 parts of monarch drugs, 67.5 parts of ministerial drugs, 6 parts of adjuvant drugs and 15 parts of conductant drugs in parts by weight, the monarch drugs comprise eucheuma, the ministerial drugs comprise safflower, red paeony root, chinese angelica tail, ginseng, poria cocos and bighead atractylodes rhizome, the adjuvant drugs comprise dried ginger, cinnamon and clove, and the conductant drugs are liquorice.
Further, the functional material comprises the following components in parts by weight: 13-17 parts of kylin, 5-7 parts of safflower, 11-13 parts of red paeony root, 11-13 parts of Chinese angelica tail, 11.5-13.5 parts of ginseng, 11.5-13.5 parts of poria cocos, 11.5-13.5 parts of bighead atractylodes rhizome, 1-3 parts of dried ginger, 1-3 parts of cinnamon, 1-3 parts of clove and 13-17 parts of liquorice.
Further, the functional material comprises the following components in parts by weight: 15 parts of kylin, 6 parts of safflower, 12 parts of red paeony root, 12 parts of Chinese angelica tail, 12.5 parts of ginseng, 12.5 parts of poria cocos, 12.5 parts of bighead atractylodes rhizome, 2 parts of dried ginger, 2 parts of cinnamon, 2 parts of clove and 15 parts of liquorice.
Further, the preparation method of the plant formula comprises the following steps:
s1, weighing the functional objects according to the weight part ratio;
s2, uniformly mixing the functional substance with deionized water according to a ratio of 1:10, refluxing with hot water at 100 ℃ for 2 hours, cooling, and filtering to obtain filtrate;
and S3, compounding the filtrate and the glycerol according to the ratio of 7:3, and then finely filtering to obtain the plant formula stock solution.
The plant composition with antioxidant and antiaging effects is used for preparing skin care products and cosmetics.
After the technical scheme is adopted, compared with the background technology, the invention has the following advantages:
the plant formula of the invention utilizes the Chinese herbal medicine extract as the effective component, contains abundant flavone active components, has the effects of antioxidation and aging resistance, can be used as an environment-friendly, safe and natural skin care product raw material, and has the dual effects of nutrition and curative effect;
the kylin is used as a rare traditional Chinese medicine material, has the effects of activating blood circulation to dissipate blood stasis, resisting bacteria and diminishing inflammation and promoting tissue restoration, is synergistic with safflower, red paeony root and angelica tail, combines the sedative effect of ginseng, poria cocos and bighead atractylodes rhizome, acts on skin, can promote the migration of epidermal cells, remarkably promote the proliferation of fibroblasts, increase the elasticity of skin and slow down the formation of wrinkles, has the effects of resisting oxidation and aging, has the advantages of warming and having no side effect, can be directly contacted with the skin when being applied to the preparation of skin care products and cosmetics, and is economical, safe and healthy.
Drawings
FIG. 1 is a graph showing the results of COL-I test on a plant formulation of the present invention;
FIG. 2 is a graph showing the results of MMP-1 detection by the plant formulation of the present invention.
Detailed Description
The present invention will be described in further detail with reference to the drawings and examples, in order to make the objects, technical solutions and advantages of the present invention more apparent. It should be understood that the specific embodiments described herein are for purposes of illustration only and are not intended to limit the scope of the invention.
Examples
The plant formula with the effects of resisting oxidization and aging comprises the following components in parts by weight: 15 parts of kylin, 6 parts of safflower, 12 parts of red paeony root, 12 parts of Chinese angelica tail, 12.5 parts of ginseng, 12.5 parts of poria cocos, 12.5 parts of bighead atractylodes rhizome, 2 parts of dried ginger, 2 parts of cinnamon, 2 parts of clove and 15 parts of liquorice.
The preparation method of the plant formula comprises the following steps:
s1, weighing the functional objects according to the weight part ratio;
s2, uniformly mixing the functional substance with deionized water according to a ratio of 1:10, refluxing with hot water at 100 ℃ for 2 hours, cooling, and filtering to obtain filtrate;
and S3, compounding the filtrate and the glycerol according to the ratio of 7:3, and then finely filtering to obtain the plant formula stock solution.
The plant formula is applied to the preparation of skin care products and cosmetics.
Experiment detection of the content of Total Flavonoids active ingredient in a plant formulation
1. Precisely weighing 0.1g of rutin reference substance, dissolving with 75% ethanol by volume fraction, and fixing volume to 100mL volumetric flask, wherein the mass concentration of rutin solution is 1.0g/L.
2. Precisely sucking the rutin solution 0.0mL,2.0mL,4.0mL,6.0mL,8.0mL and 10.0mL into a 10mL volumetric flask, diluting with 75% ethanol to scale, and shaking to obtain reference solution with mass concentration of 0.0mg/mL,2.0mg/mL,4.0mg/mL,6.0mg/mL,8.0mg/mL and 10.0 mg/mL.
3. And (3) determination of a reference substance: respectively taking 1mL of reference substance solutions with different concentrations into a 10mL volumetric flask, sequentially adding 4mL of deionized water and 0.3mL of NaNO with mass fraction of 5% 2 Adding 0.3mL of Al (NO) with mass fraction of 10% into the aqueous solution after 5min 3 ) 3 2mL of NaOH aqueous solution with mass fraction of 1mol/L and 2.4mL of deionized water are sequentially added after the aqueous solution reacts for 6min, and absorbance is measured at 510nm after 10 min; and drawing a standard curve by taking the absorbance value A as an ordinate and the rutin reference substance concentration as an abscissa.
4. Sample measurement: taking 1mL of plant formula stock solution, sequentially adding 4mL of deionized water and 0.3mL of NaNO with mass fraction of 5% into a test tube 2 Adding 0.3mL of Al (NO) with mass fraction of 10% into the aqueous solution after 5min 3 ) 3 After the reaction for 6min, 2mL of NaOH aqueous solution with mass fraction of 1mol/L and 2.4mL of deionized water are sequentially added, and absorbance is measured at 510nm after 10 min.
Experimental results: the total flavone content in the plant formula can reach 0.37mg/mL.
Anti-radical detection of experimental two-plant formulations
Measurement of DPPH radical scavenger
1. Preparing a DPPH ethanol solution: 20mg DPPH is weighed, added with absolute ethyl alcohol to be dissolved, and is stored in a 250mL volumetric flask at 0-4 ℃ in a dark place, and is effective in 4 hours after being prepared.
2. Preparing a liquid to be tested: and (3) taking the plant formula stock solution in the experiment I, and diluting to obtain the solution to be tested with the concentration of 2.5%, 5% and 10% of the stock solution. The positive control is 1mg/mL vitamin C, and is stored at 0-4deg.C in dark place for use.
3. The specific experimental steps are as follows: the reagents were added according to the following table feed-to-liquid ratios.
| Numbering device | DPPH solution | Absolute ethyl alcohol | Solution to be measured | Total volume of |
| A pipe | 1mL | —— | 1mL | 2mL |
| B pipe | 1mL | 1mL | —— | 2mL |
| C pipe | —— | 1mL | 1mL | 2mL |
After 30min of reaction, absorbance values of A, B, C tubes were measured with a microplate reader at 517 nm. The clearance rate calculation formula is: clearance (%) = [ (b+c) -a ]/B
4. Analysis of results:
the DPPH clearance rates of the plant compositions with different concentrations are shown in the following table 1.1:
TABLE 1.1 DPPH clearance of different concentration plant formulations
The experimental results in the table 1.1 show that the plant formula can obviously inhibit DPPH free radical and has obvious antioxidation effect.
(II) determination of ABTS radical scavenger
1. ABTS aqueous solution configuration: 0.03841g of ABTS is precisely weighed, dissolved and fixed in 10mL of water;
2. K2S2O8 aqueous solution configuration: accurate weighing 0.0662g K 2 S 2 O 8 Dissolving and fixing the solution in 100mL of water;
3. mother liquor preparation: mixing the above ABTS aqueous solution with K 2 S 2 O 8 Mixing the aqueous solution in equal volume, and carrying out low-temperature light-shielding reaction for 12-16 h;
4. ABTS working solution configuration: the mother solution was diluted with absolute ethanol to an OD of 0.7±0.02 at 734nm and prepared as-is.
5. Preparing a liquid to be tested: and diluting the plant formula stock solution to obtain a solution to be tested with the concentration of 2.5%, 5% and 10% of the stock solution. The positive control is 1mg/mL vitamin C, and is stored at 0-4deg.C in dark place for use.
6. The specific experimental steps are as follows:
the following reagents are added according to the ratio of the feed liquid in the table:
| numbering device | Sample of | Water and its preparation method | ABTS reagent |
| Experimental group (A) | 0.2mL | --- | 0.8mL |
| Blank group (A0) | --- | 0.2mL | 0.8mL |
The reaction was carried out at room temperature for 30min under dark conditions, and the absorbance at 734nm was measured using an ELISA reader. ABTS radical clearance: clearance (%) = [ (A0-a)/A0 ]. 100%.
7. Analysis of results:
the ABTS clearance rates of the plant formulations at different concentrations are shown in table 1.2 below:
TABTS clearance from different concentration plant formulations
The experimental results in the table 1.2 show that the plant formula can obviously inhibit ABTS free radicals and has obvious antioxidation effect.
Detection of influence of Experimental three-plant formula on synthesis of type I collagen and matrix metalloproteinase 1
The experiment is carried out by a fibroblast type I collagen synthesis promotion experiment, and the influence of the plant formula on the synthesis of type I collagen and matrix metalloproteinase 1 is detected.
Measurement of fibroblast Activity by CCK-8 method
1. Cell inoculation: according to 1E 4 Cell/well seeding Density cells were seeded into 96 well plates, incubator (37 ℃,5% CO) 2 ) Incubate overnight.
2. Preparing liquid: the experiment set up solvent control group, sample group and zeroing group. The plant formulation was diluted to 5 concentrations of 2.5%, 5%, 10%, 20%, 40% etc. with serum-free medium and 3 replicate wells were placed at each concentration gradient.
3. Administration: and when the cell plating rate in the 96-well plate reaches 80% -90%, the administration is carried out. 200. Mu.L of culture solution was added to each well of the solvent control group; 200 mu L of culture solution containing a corresponding concentration of a sample is added into each hole of the sample group; the zeroed group was inoculated without cells, and only 200. Mu.L of cell culture medium was added. After completion of the administration, the 96-well plate was placed in an incubator (37 ℃,5% co 2) for culturing.
4. After incubation of the cells for 24h, 10. Mu.L of CCK-8 working solution was added to each well, incubated at 37℃for 1h in the absence of light, and OD values were read at 450 nm.
5. Cell relative viability calculation: according to the calculation of the formula,
6. Cytotoxicity test results are shown in table 1.3 below:
TABLE 1.3 cytotoxicity test results
The experimental results in table 1.3 above show that the plant formulation has a proliferative effect on fibroblasts. Sample concentrations of 2.5%, 5% and 10% were selected for type I collagen and matrix metalloproteinase 1 detection experiments.
(II) type I collagen and matrix metalloproteinase 1 detection
1. Inoculating: according to 6E 5 Cell/well seeding density cells were seeded into 6-well plates and incubated overnight in incubator (37 ℃,5% co 2).
2. UVB irradiation: when the cell plating rate in the 6-hole plate reaches 80% -90%, the culture solution is discarded, 1mL of PBS solution is added into each hole, the cells are washed, the washing is repeated for 2 times, and then a proper amount of PBS is added to cover the cells, UVB radiation is carried out, and the radiation dose is 50mJ/cm 2 。
3. Administration: the experiments set up blank, UVB, sample.
The blank group was irradiated without UVB and was inoculated with 2mL of medium.
After UVB irradiation, the UVB group was inoculated with 2mL of medium.
After UVB irradiation, the drug groups were inoculated with 2mL of sample media (2.5%, 5%, 10%) of different concentrations. 37 ℃,5% CO 2 The incubator continues to incubate for 24 hours.
4. Collecting supernatant: after 24h of incubation, the cell culture supernatant was collected in an EP tube and stored in a freezer at-80 ℃.
5. Detection of type I collagen (COL-I) content: cell samples were tested according to the instructions of the COL-I ELISA kit.
6. Matrix metalloproteinase 1 (MMP-1): cell samples were tested according to MMP-1ELISA kit protocol.
7. Statistical analysis of results
Test results were plotted using GraphPadPrism Program software, and the sample groups were compared to the negative control groups, using SPSS statistical analysis, p <0.05 (x) indicated significant differences, and p <0.01 (x) indicated very significant differences.
(1) COL-I test results
According to the ELISA kit instructions, COL-I content detection was performed, and the detection results are shown in Table 1.4 below.
TABLE 1.4 COL-I detection results
Table 1.4 above yields histograms as shown in FIG. 1, and FIG. 1 shows COL-I test results for plant formulations. Compared with a blank group, the COL-I content of the UVB group is extremely obviously reduced, which proves that the UVB stimulation condition of the experiment is effective; the increased COL-I content in the sample group compared to the UVB group indicates that the sample has a promoting effect on the synthesis of type I collagen.
(2) MMP-1 assay
According to the ELISA kit specification, MMP-1 content was detected, and the detection results are shown in Table 1.5.
TABLE 1.5 MMP-1 assay results
Table 1.5 above generates histograms as shown in FIG. 2, and FIG. 2 shows MMP-1 detection results for plant formulations. Compared with a blank group, the MMP-1 content of the UVB group is extremely obviously increased, which proves that the UVB stimulation condition of the experiment is effective. Compared with the UVB group, the MMP-1 content of the sample group is reduced, which indicates that the sample has an inhibition effect on the synthesis of matrix metalloproteinase 1.
In conclusion, the plant formula can inhibit DPPH and ABTS free radical generation, promote the synthesis of type I collagen and inhibit the secretion of matrix metalloproteinase 1, and is a plant efficacy raw material with oxidation resistance and anti-wrinkle effects.
The present invention is not limited to the above-mentioned embodiments, and any changes or substitutions that can be easily understood by those skilled in the art within the technical scope of the present invention are intended to be included in the scope of the present invention. Therefore, the protection scope of the present invention should be subject to the protection scope of the claims.
Claims (5)
1. A plant formula with antioxidant and anti-aging effects is characterized in that: the traditional Chinese medicine composition comprises, by weight, functional materials, deionized water and glycerin in a ratio of 7:70:30, wherein the functional materials comprise, by weight, 15 parts of monarch drugs, 67.5 parts of ministerial drugs, 6 parts of adjuvant drugs and 15 parts of conductant drugs, the monarch drugs comprise kylin, the ministerial drugs comprise safflower, red paeony root, chinese angelica tail, ginseng, poria cocos and bighead atractylodes rhizome, the adjuvant drugs comprise dried ginger, cinnamon and clove, and the conductant drugs are licorice.
2. A plant formulation having antioxidant and anti-aging effects according to claim 1, wherein: the functional material comprises the following components in parts by weight: 13-17 parts of kylin, 5-7 parts of safflower, 11-13 parts of red paeony root, 11-13 parts of Chinese angelica tail, 11.5-13.5 parts of ginseng, 11.5-13.5 parts of poria cocos, 11.5-13.5 parts of bighead atractylodes rhizome, 1-3 parts of dried ginger, 1-3 parts of cinnamon, 1-3 parts of clove and 13-17 parts of liquorice.
3. A plant formulation having antioxidant and anti-aging effects as claimed in claim 2, wherein: the functional material comprises the following components in parts by weight: 15 parts of kylin, 6 parts of safflower, 12 parts of red paeony root, 12 parts of Chinese angelica tail, 12.5 parts of ginseng, 12.5 parts of poria cocos, 12.5 parts of bighead atractylodes rhizome, 2 parts of dried ginger, 2 parts of cinnamon, 2 parts of clove and 15 parts of liquorice.
4. A plant formulation having antioxidant and anti-aging effects as claimed in claim 3, wherein: the preparation method of the plant formula comprises the following steps:
s1, weighing the functional objects according to the weight part ratio;
s2, uniformly mixing the functional substance with deionized water according to a ratio of 1:10, refluxing with hot water at 100 ℃ for 2 hours, cooling, and filtering to obtain filtrate;
and S3, compounding the filtrate and the glycerol according to the ratio of 7:3, and then finely filtering to obtain the plant formula stock solution.
5. An application of a plant formula with antioxidant and anti-aging effects is characterized in that: the use of a plant formulation according to any one of claims 1 to 4 for the preparation of skin care products, cosmetics.
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