CN112656924A - A Chinese medicinal composition with antiinflammatory, analgesic, and antibacterial effects, and its preparation method - Google Patents
A Chinese medicinal composition with antiinflammatory, analgesic, and antibacterial effects, and its preparation method Download PDFInfo
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Abstract
The invention relates to a traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria, which comprises dried ginger, turmeric, pepper, lost stick, wormwood, rhizoma gastrodiae, dried orange peel, green tangerine peel, notopterygium root, clematis root, combined spicebush root, phellodendron bark, teasel root, red paeony root, liquorice, Chinese angelica, Szechuan lovage rhizome, Japanese ampelopsis root, manchurian wildginger, raw bitter orange, chaulmoogratree seed, raw eucommia bark, raw aconite root, costus root, raw stiff silkworm, raw fennel, raw monkshood root, raw kusnezoff monkshood root, raw peach kernel, raw polygala root, garden balsam stem, orientvine stem, common cnidium fruit, achyranthes root, south dodder seed, raw nutgrass galingale rhizome; the Chinese medicinal composition can be used for preventing and treating soreness of waist and legs, difficulty in flexion and extension of joint, and relieving pain caused by traumatic injury, rheumatic arthritis, rheumatoid arthritis, and malignant tumor.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicines, and particularly relates to an anti-inflammatory analgesic or antibacterial traditional Chinese medicine composition and a preparation method thereof.
Background
The application therapy is a traditional Chinese medicine external therapy which directly acts the traditional Chinese medicine preparation on the skin, acupuncture points and pathological change parts of patients under the guidance of the traditional Chinese medicine theory, can exert local treatment effect by non-oral methods such as stimulating channels and collaterals and acupuncture points, transdermal absorption and the like, and can even achieve the treatment effect of the whole body by skin or mucosa absorption. The therapy is not taken orally and is not directly injected into the body, has the characteristics of safe and convenient use, little side effect and less burden on the body, is easily accepted by doctors, is widely applied to surgical, orthopedic, dermatologic, ENT, anorectal, internal medicine and gynecological diseases, and particularly has the advantages of oral administration and injection administration methods for the old, children and other people who are inconvenient to take and are not suitable for taking medicine due to physical reasons or can not take medicine. Transdermal drug delivery systems associated with patch therapy have been shown to have unique therapeutic effects on a number of diseases, and some plasters have been used for analgesia in the treatment of advanced malignancies. In addition, products such as waist support, shoulder support, eye protection, neck support, knee protection, etc. developed based on the principle of the application therapy are also gaining popularity in the market.
Disclosure of Invention
The invention aims to overcome the defects of the prior art and provides a traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria. The Chinese medicinal composition has antiinflammatory, repercussive, analgesic, and antibacterial effects, and can be used for preventing and treating soreness of waist and legs, difficulty in flexion and extension of joint, and relieving pain caused by traumatic injury, rheumatic arthritis, and rheumatoid arthritis, and malignant tumor.
The invention also provides a preparation method of the traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria.
In order to achieve the purpose, the invention adopts the following technical scheme:
a traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria mainly comprises the following raw material medicines in parts by weight:
60-120 parts of dried ginger, 20-40 parts of turmeric, 20-40 parts of pepper, 20-40 parts of ramulus et folium Claoxyli, 20-40 parts of wormwood, 20-40 parts of rhizoma Gastrodiae, 5-20 parts of dried orange peel, 5-20 parts of green tangerine peel, 5-20 parts of notopterygium root, 5-20 parts of radix Clematidis, 5-20 parts of radix Linderae, 5-20 parts of cortex Phellodendri, 5-20 parts of radix Dipsaci, 5-20 parts of radix Paeoniae Rubra, 5-20 parts of licorice, 5-20 parts of Angelica sinensis, 5-20 parts of Ligusticum wallichii, 5-20 parts of radix Ampelopsis, 5-20 parts of asarum, 5-20 parts of raw fructus Aurantii, 5-20 parts of chaulmoogra seed, 5-20 parts of raw eucommia bark, 5-20 parts of raw radix Aconiti lateralis, 5-20 parts of costus root, 5-20 parts of raw Bombyx Batryticatus, 5-20 parts of raw fennel, 5, 5-20 parts of raw peach kernels, 5-20 parts of raw polygala tenuifolia, 5-20 parts of garden balsam stems, 5-20 parts of caulis sinomenii, 5-20 parts of common cnidium fruits, 5-20 parts of radix achyranthis bidentatae, 5-20 parts of Chinese dodder seeds, 5-20 parts of raw rhizoma cyperi, 5-20 parts of raw bighead atractylodes rhizome, 5-20 parts of raw szechwan chinaberry fruits, 5-20 parts of cinnamon, 5-20 parts of camphor, 5-20 parts of dragon's blood, 5-20 parts of catechu, 5-20 parts of myrrh, 5-20 parts of frankincense and 5-20 parts of flos caryophylli.
The invention provides a preparation method of the traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria, which comprises the following steps: proportionally placing the other raw materials except cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli in an extraction tank, extracting with 70-80% ethanol under heating and refluxing, filtering the extractive solution (300 mesh), and concentrating under reduced pressure to relative density of 1.4-1.6; adding cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli powder, mixing, and concentrating to obtain Chinese medicinal composition paste.
In the preparation process of the traditional Chinese medicine composition, 7 medicines of cinnamon, camphor, dragon's blood, catechu, myrrh, frankincense and flos caryophyllata contain volatile components, so that the traditional Chinese medicine composition is not suitable for being extracted together with other raw material medicines.
Further, the other raw materials except for cinnamon, camphor, dragon's blood, catechu, myrrh, frankincense and flos caryophyllata are mixed with 70-80% ethanol according to the solid-to-liquid ratio of 1 g: adding 5-8 ml; reflux extracting for 2-4 times (extraction temperature is 70-90 deg.C), each time for 2-4 hr. Vacuum concentrating at 50-70 deg.c and-0.07-0.09 MPa to recover solvent.
The invention also provides a preparation method of the anti-inflammatory analgesic or antibacterial traditional Chinese medicine composition hot-melt pressure-sensitive adhesive plaster, which comprises the following steps:
1) taking the other raw materials except cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli at a certain proportion, placing in an extraction tank, extracting with 70-80% ethanol under heating and refluxing, filtering the extractive solution, and concentrating under reduced pressure to relative density of 1.4-1.6 to obtain paste for use;
2) melting 1000 parts of hot melt pressure sensitive adhesive at 300 deg.C, cooling to 175 deg.C, adding 2.4kg of paste, and powders of cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli, and mixing; and coating the fabric with a coating machine, wherein the coating thickness is 0.5mm, the surface is covered with ion paper, and the fabric is cut into patches with the length and the width of 8 x 12cm, namely the hot melt pressure sensitive adhesive patches.
A preparation method of the traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria comprises the following steps:
1) proportionally placing the other raw materials except cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli in an extraction tank, extracting with 70-80% ethanol under heating and refluxing, filtering the extractive solution, and concentrating under reduced pressure to relative density of 1.4-1.6; adding cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli powder, mixing to obtain concentrated unguent of Chinese medicinal composition;
2) weighing 20 parts of the traditional Chinese medicine composition concentrated paste, 10 parts of anhydrous lanolin and 100 parts of vaseline for later use;
3) placing the concentrated Chinese medicinal composition paste into a mortar, adding sterilized vaseline and anhydrous lanolin cooled to 55-65 deg.C, and grinding.
In addition, the traditional Chinese medicine composition can be prepared into a medicinal preparation together with conventional pharmaceutic adjuvants in pharmaceutics. The medicinal preparation can be external patch, external ointment, liniment, cataplasm, etc., can be made into patch of different shapes such as knee patch, cervical vertebra patch, navel patch, heel patch, foot patch, nose patch, acupoint patch, etc. according to different application parts of knee, cervical vertebra, lumbar vertebra, navel patch, heel patch, foot patch, nose patch, etc., and has effects of resisting inflammation, dredging collaterals, promoting blood circulation, dispelling cold, eliminating dampness, relieving pain, and treating diseases such as acute soft tissue injury, acute synovitis, abdominal pain, external tumor, etc.
The traditional Chinese medicine composition for external treatment disclosed by the invention takes the dried ginger as a monarch drug in the prescription, and the dried ginger can increase local blood circulation to dissolve wind cold and stimulate hair follicles to open so as to play the roles of dispelling cold, relieving pain and removing moisture.
Turmeric, pepper, lotus seed, wormwood and gastrodia tuber are used as ministerial drugs. Wherein, the turmeric has the effects of breaking blood and promoting qi circulation, and dredging channels and relieving pain, and is suitable for symptoms such as rheumatism shoulder and arm pain, traumatic swelling pain and the like; pepper is pungent in taste and warm in nature, has the effects of warming the middle-jiao to dispel cold, descending qi and dissolving phlegm, and has activity in various aspects such as analgesia, sedation, cytoprotection, anti-inflammation, bacteriostasis, disinsection, anti-tumor and the like according to modern pharmacology; the medicine is not thrown with a stick, has pungent and slightly bitter taste, has small toxicity, mild nature, and has the effects of expelling wind and removing dampness, and reducing swelling and relieving pain, and the medicine recorded in the raw herbal medicine property preparation records that the medicine has the effects of dispelling wind and removing dampness, and reducing swelling and relieving pain, is used for treating rheumatic arthritis, lumbocrural pain, traumatic swelling and pain, beriberi edema, and can also be used for treating burn, scald and traumatic hemorrhage. The wormwood is pungent and bitter in taste, warm in nature, enters liver, spleen and kidney meridians, has the effects of warming meridians, removing dampness, dispelling cold, stopping bleeding, diminishing inflammation, relieving asthma, relieving cough, preventing miscarriage, resisting allergy and the like, can eliminate dampness and relieve itching when being externally used, and is recorded as a main hemostatic medicine by doctors in past; gastrodia elata, a common medicine for dispelling wind and eliminating dampness and warming and activating meridians and collaterals, is applied to traditional Chinese herbs in all ages, for example, Gastrodia elata recorded in Kaibao Bencao has the effects of treating various kinds of rheumatism, treating spasm of limbs … …, benefiting waist and knee, strengthening tendons and strengthening force, so that Gastrodia elata recorded in Yaobao Bencao has the effects of reducing body weight, activating meridian and benefiting waist and knee, and regulating blood vessels, and so on. The five medicines play the roles of dispelling cold, expelling wind, removing dampness, dredging collaterals, relieving pain and resisting allergy together, are essential in the formula.
Pericarpium Citri Tangerinae, pericarpium Citri Reticulatae viride, Notopterygii rhizoma, radix Clematidis, radix Linderae, cortex Phellodendri, radix Dipsaci, radix Paeoniae Rubra, Glycyrrhrizae radix, radix Angelicae sinensis, rhizoma Ligustici Chuanxiong, radix Ampelopsis, herba asari, fructus Aurantii, semen Hydnocarpi, Eucommiae cortex, radix Aconiti lateralis Preparata, radix aucklandiae, Bombyx Batryticatus, fructus Foeniculi, radix Aconiti Kusnezoffii, semen Persicae, radix Polygalae, herba Speranskiae Tuberculatae, caulis Sinomenii, fructus Cnidii, semen Cuscutae, rhizoma Cyperi, Atractylodis rhizoma, fructus Toosendan, cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum, and flos Caryophylli as adjuvant drugs. Achyranthes root, radix Achyranthis bidentatae, as a guiding drug, has the effects of removing blood stasis, stimulating the menstrual flow, tonifying liver and kidney, strengthening tendons and bones, and inducing blood to descend.
The invention adopts a mouse plantar swelling and pain model (Parasto Mojtahed, Zadeh-Ardabilia, Sima Kianpoor Rad, anti-pain and anti-inflammation effects of Neptune krill oil and fat oil induced inflammation in mice: Current states and pilot results [ J ] Biotechnology Reports,2019,22:1-7.) to test and observe the prevention and treatment effects of the external application curative effect of the traditional Chinese medicine composition provided by the invention. The experimental results show that: after the traditional Chinese medicine composition (one gram of ointment is equivalent to 3.2 grams of raw medicinal materials) provided by the invention is administered, the sole swelling and pain index of a mouse with inflammatory pain can be obviously reduced.
The invention adopts mouse auricle swelling models (Huang-he Yu, Ye Lin, Rong Zeng, et al, analytical and anti-inflammatory effects and molecular mechanisms of Kadsura hectorita steps, an anti-clinical Chinese tube ethnomedicinal herb [ J ]. Journal of Ethnopharmacology,2019,238(28):1-8.) to experimentally observe the prevention and treatment effects of the external application curative effect of the traditional Chinese medicine composition provided by the invention. The experimental results show that: after the traditional Chinese medicine composition provided by the invention is administrated, the auricle swelling pain index of a mouse with inflammation pain can be obviously reduced.
Compared with the prior art, the technical scheme of the invention has the following obvious advantages:
1) the traditional Chinese medicine composition has the effects of resisting inflammation, reducing swelling, relieving pain and resisting bacteria, has the characteristics of obvious curative effect, less side effect in clinical application, wide application population, convenience and quickness in use and the like, and can be used for preventing and treating waist and leg ache and joint flexion and extension difficulty, and relieving joint swelling and pain and malignant tumor pain caused by traumatic injury and rheumatic and rheumatoid arthritis;
2) the invention adopts a formaldehyde foot licking experiment and a mouse auricle xylene inflammation experiment to observe the prevention and treatment effects of the traditional Chinese medicine composition on pain and inflammation. The test results show that: has good anti-inflammatory and analgesic effects on the model mouse.
Detailed Description
The technical solution of the present invention is further described in detail with reference to the following examples, but the scope of the present invention is not limited thereto.
In the following examples, the parts of the raw materials added are all parts by weight unless otherwise specified.
Example 1
A preparation method of the ointment of the traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria comprises the following specific steps:
1) preparing an extracting solution: 80 parts of dried ginger, 30 parts of turmeric, 30 parts of pepper, 30 parts of ramulus et folium Claoxyli, 30 parts of wormwood, 30 parts of rhizoma Gastrodiae, 15 parts of dried orange peel, 15 parts of green tangerine peel, 15 parts of notopterygium root, 15 parts of radix clematidis, 15 parts of radix linderae, 15 parts of golden cypress, 15 parts of radix dipsaci, 15 parts of radix paeoniae rubra, 15 parts of liquorice, 15 parts of angelica sinensis, 15 parts of ligusticum wallichii, 15 parts of radix ampelopsis, 15 parts of asarum, 15 parts of raw fructus aurantii, 15 parts of chaulmoogra, 15 parts of raw eucommia ulmoides, 15 parts of raw monkshood, 15 parts of costus root, 15 parts of raw stiff silkworm, 15 parts of raw fennel, 15 parts of raw radix aconiti agrestis, 15 parts of raw peach kernel, 15 parts of raw polygala tenuifolia, 15 parts of garden balsam stem, 15 parts of caulis sino; placing the raw materials in an extraction tank, and extracting with 6 times (v/w) of 75% ethanol under heating and refluxing for 3 times (i.e. adding the raw materials and 75% ethanol at a solid-to-liquid ratio of 1 g: 6 ml), each time for 2 hr;
2) concentrating into paste: mixing the extractive solutions for 3 times, filtering (300 mesh), concentrating the filtrate at 60 deg.C under reduced pressure (-0.09MPa) to recover solvent, decocting to obtain paste with relative density of about 1.5, adding cortex Cinnamomi powder (sieved with 300 mesh sieve) 15 parts, Camphora powder 15 parts, sanguis Draxonis powder 15 parts, Catechu powder 15 parts, Myrrha powder 15 parts, Olibanum powder 15 parts, and flos Caryophylli powder 15 parts, and mixing to obtain concentrated paste of Chinese medicinal composition.
Example 2:
a preparation method of a medical hot-melt pressure-sensitive adhesive plaster of a traditional Chinese medicine composition comprises the following steps:
1) weighing 80 parts of dried ginger, 30 parts of turmeric, 30 parts of pepper, 30 parts of ramulus et folium Claoxyli, 30 parts of wormwood, 30 parts of rhizoma Gastrodiae, 15 parts of dried orange peel, 15 parts of green tangerine peel, 15 parts of notopterygium root, 15 parts of radix clematidis, 15 parts of radix linderae, 15 parts of golden cypress, 15 parts of radix dipsaci, 15 parts of radix paeoniae rubra, 15 parts of liquorice, 15 parts of angelica sinensis, 15 parts of ligusticum wallichii, 15 parts of radix ampelopsis, 15 parts of asarum, 15 parts of raw fructus aurantii, 15 parts of chaulmoogra, 15 parts of raw eucommia ulmoides, 15 parts of raw monkshood, 15 parts of costus root, 15 parts of raw stiff silkworm, 15 parts of raw fennel, 15 parts of raw radix aconiti agrestis, 15 parts of raw peach kernel, 15 parts of raw polygala tenuifolia, 15 parts of garden balsam stem, 15 parts of caulis; pulverizing the above materials, mixing, and sieving with 350 mesh sieve to obtain Chinese medicinal powder;
2) melting 1000 parts of hot melt pressure sensitive adhesive (produced by Shanghai Tiandiao new material science and technology Co., Ltd.) at 300 deg.C, adding the above Chinese medicinal composition powder, rapidly stirring to mix well, standing for 4 hr, and cooling to 175 deg.C. Mixing and crushing 7 medicines of 15 parts of cinnamon, 15 parts of camphor, 15 parts of dragon's blood, 15 parts of catechu, 15 parts of myrrh, 15 parts of frankincense, 15 parts of cloves and the like, sieving the mixture by a 300-mesh sieve, adding the mixture, quickly and uniformly stirring the mixture, then coating the mixture on a piece of woven fabric by a coating machine, wherein the coating thickness is 0.5mm, the surface of the woven fabric is covered by ion paper, and then cutting the woven fabric into patches with the length and the width of 8 x 12cm to obtain the plaster.
Example 3:
a preparation method of a hot-melt pressure-sensitive adhesive plaster of a traditional Chinese medicine composition comprises the following steps:
prescription: 80 parts of dried ginger, 30 parts of turmeric, 30 parts of pepper, 30 parts of ramulus et folium Claoxyli, 30 parts of wormwood, 30 parts of rhizoma Gastrodiae, 15 parts of dried orange peel, 15 parts of green tangerine peel, 15 parts of notopterygium root, 15 parts of radix clematidis, 15 parts of radix linderae, 15 parts of golden cypress, 15 parts of teasel root, 15 parts of red peony root, 15 parts of liquorice, 15 parts of angelica sinensis, 15 parts of ligusticum wallichii, 15 parts of radix ampelopsis, 15 parts of asarum, 15 parts of raw fructus aurantii, 15 parts of chaulmoogra, 15 parts of raw eucommia bark, 15 parts of raw monkshood, 15 parts of costus root, 15 parts of raw stiff silkworm, 15 parts of raw fennel, 15 parts of raw radix aconiti, 15 parts of raw kusnezoff monkshood root, 15 parts of raw peach kernel, 15 parts of raw polygala tenuifolia, 15 parts of garden balsam stem, 15 parts of caulis sinomenii, 15 parts of fructus cnidii, 15 parts of achyranthis bidentatae, 15 parts of semen cuscutae, 15 parts of.
The preparation method specifically comprises the following steps:
1) taking other raw materials except cinnamon, camphor, dragon's blood, catechu, myrrh, frankincense and flos caryophyllata according to a certain proportion, placing the raw materials into an extraction tank, heating and refluxing for 3 times by using 6 times (v/w) of 75% ethanol (namely, the raw materials and the 75% ethanol are mixed according to a solid-to-liquid ratio of 1 g: 6ml addition), 2 hours each time; mixing the extractive solutions, filtering (300 mesh), concentrating the filtrate at 60 deg.C under reduced pressure (-0.09MPa) to recover solvent, and decocting to relative density of about 1.5 to obtain paste;
2) melting 1000 parts of hot-melt pressure-sensitive adhesive at 300 ℃, cooling to 175 ℃, adding the ointment, mixing and crushing 7 medicines of 15 parts of cinnamon, 15 parts of camphor, 15 parts of dragon's blood, 15 parts of catechu, 15 parts of myrrh, 15 parts of frankincense, 15 parts of flos caryophyllata and the like, sieving with a 300-mesh sieve, adding the mixture, and quickly and uniformly stirring. Coating the mixture on a woven fabric by using a coating machine, wherein the thickness is 0.5mm, the surface of the woven fabric is covered with ion paper, and then cutting the woven fabric into patches with the length and the width of 8 x 12cm to obtain the adhesive patches.
Example 4:
a topical Chinese medicinal ointment and its preparation method are provided, which comprises:
prescription: 65 parts of dried ginger, 20 parts of turmeric, 20 parts of pepper, 20 parts of ramulus et folium Claoxyli, 40 parts of wormwood, 40 parts of rhizoma Gastrodiae, 10 parts of dried orange peel, 20 parts of green tangerine peel, 10 parts of notopterygium root, 10 parts of radix clematidis, 10 parts of radix linderae, 20 parts of cortex phellodendri, 20 parts of radix dipsaci, 20 parts of radix paeoniae rubra, 10 parts of liquorice, 10 parts of angelica sinensis, 10 parts of ligusticum wallichii, 10 parts of radix ampelopsis, 10 parts of asarum, 10 parts of raw fructus aurantii, 10 parts of chaulmoogra, 10 parts of raw eucommia ulmoides, 12 parts of raw monkshood, 20 parts of costus root, 20 parts of raw stiff silkworm, 20 parts of raw fennel, 20 parts of raw radix aconiti, 20 parts of raw kusnezoff monkshood root, 20 parts of raw peach kernel, 20 parts of raw polygala tenuifolia, 20 parts of garden balsam stem, 20 parts of caulis sinomenii acutissimonstratae, 20 parts of fructus cnidii, 20 parts of achyranthis bidentatae. The preparation method specifically comprises the following steps:
1) weighing 20 parts of the traditional Chinese medicine composition concentrated paste prepared by the method of the embodiment 1, 10 parts of anhydrous lanolin and 100 parts of vaseline for later use;
2) placing the concentrated Chinese medicinal composition paste into a mortar, adding sterilized vaseline and anhydrous lanolin cooled to 55-65 deg.C, and grinding.
Example 5:
a Chinese medicinal composition hydrogel and its preparation method are as follows:
prescription: 110 parts of dried ginger, 40 parts of turmeric, 40 parts of pepper, 20 parts of ramulus et folium Claoxyli, 20 parts of wormwood, 20 parts of rhizoma Gastrodiae, 20 parts of dried orange peel, 10 parts of green tangerine peel, 20 parts of notopterygium root, 20 parts of radix clematidis, 20 parts of radix linderae, 10 parts of cortex phellodendri, 10 parts of radix dipsaci, 10 parts of radix paeoniae rubra, 20 parts of liquorice, 20 parts of angelica sinensis, 20 parts of ligusticum wallichii, 20 parts of radix ampelopsis, 20 parts of asarum, 20 parts of raw fructus aurantii, 20 parts of chaulmoogra, 20 parts of raw eucommia ulmoides, 8 parts of raw monkshood, 10 parts of costus root, 10 parts of raw stiff silkworm, 10 parts of raw fennel, 10 parts of raw radix aconiti agrestis, 10 parts of raw peach kernel, 10 parts of raw polygala tenuifolia, 10 parts of garden balsam stem, 10 parts of caulis sinomenii acutissimonii, 10 parts of fructus cnidii, 10 parts of achyranthis bidentatae, 20 parts of. The preparation method specifically comprises the following steps:
1) weighing 10 parts of the concentrated ointment of the traditional Chinese medicine composition prepared by the method of the embodiment 1, 10 parts of cross-linked sodium polyacrylate (SDB-L-400, produced by Zhengzhou Bill chemical products Co., Ltd.), PEG-400080 parts and 100 parts of glycerol for later use;
2) placing PEG-4000 and glycerol in a beaker, heating to dissolve completely, adding the concentrated paste of the Chinese medicinal composition, and mixing;
3) adding 800 parts of water (60 ℃) into SDB-L-400, and uniformly grinding in a mortar to prepare a hydrogel matrix;
4) and (3) uniformly mixing the hydrogel matrix obtained in the step 3) and the product obtained in the step 2).
Note: SDB-L-400 is a high water-absorbing resin material, can absorb 200-300 times of self weight in 90 seconds and can expand into a colloidal semi-solid, and the embodiment takes the SDB-L-400 as a substrate, so that the SDB-L-400 can play roles in moisturizing, thickening and infiltrating skin; PEG-4000 is used as transdermal absorption enhancer.
Application test 1: bacteriostatic activity experiment of traditional Chinese medicine composition
1. Experimental strains: coli ATCC 25922, Staphylococcus aureus ATCC6538 and Candida albicans ATCC10231, all purchased from Nanjing fecal Biotech Ltd.
2. The experimental method comprises the following steps: determination of bacteriostatic Activity of the concentrated paste of the Chinese medicinal composition obtained in example 1 by Filter paper sheet method
Firstly, a plurality of circular filter paper sheets with the diameter of 6mm are prepared and sterilized. Clamping with sterile forceps, respectively adding into the concentrated paste water solution of example 1 with concentration of 0.1g/mL, 0.4g/mL, and 1g/mL, soaking for 2 hr, taking out, and air drying under sterile condition. Putting the sterilized culture dishes and MH agar culture medium into a clean bench, cooling to 60 ℃, pouring plates with about 20mL of each dish, horizontally placing the culture dishes after pouring, and solidifying the culture medium. 0.2mL of the bacterial suspension was pipetted onto the plate and spread evenly with a spreading bar. The air-dried filter paper pieces are clamped by tweezers and placed on a seeded flat plate, 3 filter paper pieces are placed in each dish in an equilateral triangle shape and are parallel to each other, the dish is placed in an incubator and is inversely cultured for 24 hours at 37 ℃, and the diameter of a bacteriostatic circle is measured.
3. Judging the index: and determining the sensitivity according to the diameter of the inhibition zone. The diameter of the inhibition zone is less than 10mm, the resistance is drug resistance, the moderate sensitivity is 10-15 mm, and the high sensitivity is more than 15 mm.
4. The results are shown in Table 1.
Table 1 bacteriostatic activity of the concentrated ointment of the Chinese medicinal composition obtained in example 1 against three common bacteria
Note: the diameter of the inhibition zone in the table is the average measurement value of three parallel experiments
As can be seen from the results in table 1, the traditional Chinese medicine composition concentrated ointment obtained in example 1 is highly sensitive to three common bacteria in medium dose (0.4g/mL) and high dose (1g/mL), and the bacteriostatic effect is enhanced with the increase of the dose, which indicates that the traditional Chinese medicine composition concentrated ointment obtained in example 1 has better inhibitory effect on three common bacteria.
Application test 2: mouse formaldehyde foot licking experiment of traditional Chinese medicine composition
2.1 Experimental animals
Female mice of Kunming species were provided by Schlekschada laboratory animals Co., Ltd, Hunan (Lot #43004700058462, laboratory animal license number: SCKX (Hunan) 2016-.
2.2 Experimental modeling and drug administration
The mice were randomly divided into three groups of 20 mice each. Depilatory cream is applied to both sides of the spine of the mouse for 5min and then cleaned with gauze. The mice of each group are coated with drugs in the unhairing area (0.2 mL/mouse of physiological saline is coated in a blank control group, 0.2 g/mouse of hydrocortisone is coated in a positive control group, and 0.2 g/mouse of the concentrated ointment of the traditional Chinese medicine composition in the example 1 is coated in an experimental group), the drugs are continuously coated for 7 days, and after the mice are administered for 1h on the 7 th day, 0.04 mL/mouse of formaldehyde solution of 2.5mL/L is injected in the left rear sole, and the timing is immediately carried out.
2.3 Observation index
The cumulative time of left hind paw licking and left hind paw withdrawal of mice within 0-5min and 20-30min after injection was recorded separately and the differences between groups were compared.
2.4 results of the experiment
Statistical analysis of the experimental data was performed using SPSS 17.0 for t-test, and differences were considered statistically significant when P < 0.05. The results are shown in Table 2.
TABLE 2 Effect of groups of drugs on the Formaldehyde pain test in mice
*Comparison with blank control group P<0.05
The results in table 2 show: compared with a blank control group, the times of licking the feet of the mice in the positive control group and the experimental group are obviously reduced within a time period of 0-5min, and the times of licking the feet of the mice in the experimental group are less than those of the mice in the positive drug control group within a time period of 20-30min, which shows that the concentrated ointment of the traditional Chinese medicine composition in the embodiment 1 has good anti-inflammatory and analgesic effects on the mice in the model.
Application test 3: experiment for inflammation caused by xylene in mouse ear of traditional Chinese medicine composition
3.1 Experimental animals
Female mice of Kunming species were provided by Schlekschada laboratory animals Co., Ltd, Hunan (Lot #43004700058462, laboratory animal license number: SCKX (Hunan) 2016-.
3.2 Experimental modeling and drug administration
The mice were randomly divided into three groups of 6 mice each. Depilatory cream is applied to both sides of the spine of the mouse for 5min and then cleaned with gauze. Each group of mice is coated with drugs in a depilation region (a blank control group is coated with 0.2mL of physiological saline, a positive control group is coated with 0.2g of hydrocortisone, and an experimental group is coated with 0.2g of the traditional Chinese medicine composition concentrated paste in example 1), the drugs are continuously coated for 7 days, after the mice are administered for 1h on the 7 th day, the two surfaces of the right ear of the mice are coated with 36 mul of dimethylbenzene respectively, the left ear is used as a control, and the mice are killed after 30min by dislocation of cervical vertebrae.
3.3 Observation index
Cutting two ears along the base line of auricle, respectively punching round ear pieces at the same position with a puncher with diameter of 8mm, weighing with an electronic balance, and calculating swelling degree and swelling inhibition rate.
Swelling degree is the right ear weight-left ear weight.
The swelling inhibition rate is (average swelling degree of blank control group-average swelling degree of administration group)/average swelling degree of blank control group × 100%.
3.4 results of the experiment
Statistical analysis of the experimental data was performed using SPSS 17.0 for t-test, and differences were considered statistically significant when P < 0.05. The results are shown in Table 3.
TABLE 3 Effect of each group of drugs on xylene-induced ear swelling in mice
*Comparison with blank control group P<0.05
The results in table 3 show: compared with a blank control group, the auricle swelling degrees of the mice of the positive control group and the experimental group are obviously reduced, and the auricle swelling inhibition rate of the mice of the experimental group is higher than that of the mice of the positive control group, so that the concentrated ointment of the traditional Chinese medicine composition in the example 1 has good anti-inflammatory and swelling-reducing effects on the model mice.
Application test 4: acute soft tissue injury test
4.1 Experimental animals
SD rats were provided by Schlekstaka laboratory animals Ltd, Hunan (Lot #43004700058462, laboratory animal license number: SCKX (Hunan) 2016-.
4.2 Experimental modeling and drug administration
The left hind leg of the SD rat was clipped out of the rat 1 day before the experiment and then the left hind leg of the rat was removed with 8% sodium sulfide solution. Marking the outer side of the middle part of the left hind limb, carrying out intraperitoneal injection anesthesia (0.7 mL/rat) on the rat by using a 10% chloral hydrate solution, fixing the rat on a rat board after anesthesia, fixing a marked part on a soft tissue impactor table, vertically placing a hollow tube with the height of 100cm and the diameter of 1.2cm at the marked part, continuously striking the marked left hind limb soft tissue for 3 times by using a weight with the mass of 100g along the free falling body of the hollow tube, and causing obvious subcutaneous bleeding and swelling at the struck part. 30 rats with the weight of about 180-220 g and half male and female parts successfully modeled in a local acute soft tissue injury model are taken and randomly divided into a blank control group, a positive control group (Xiaoshangtong liniment, Chinese medicine standard Z10880032, Yang Zhongzhi pharmaceutical industry Limited responsibility company) and an experimental group, wherein each group contains 10 rats, each group takes a striking part as the center, corresponding medicines are smeared on the rats (0.2 mL/one of 0.9% physiological saline is given to the blank control group, the administration dose of the Xiaoshangtong liniment is 0.046 g/one, and the administration dose of the experimental group is 0.4 g/one of the concentrated traditional Chinese medicine composition in the example 1), taking the degree of covering the damaged part, fixedly administering the medicine for 1 time/d by using gauze and a breathable adhesive tape, continuously administering the medicine for 5 days after molding every day, removing the medicine 6 hours after the medicine is administered every day, observing the swelling and fading conditions of ecchymosis, and scoring according to a scoring standard table (shown in a table 4) of the ecchymosis of the local soft tissue injury.
TABLE 4 Scoring standard table for soft tissue injury ecchymoses
4.3 results of the experiment
Statistical analysis of the experimental data was performed using SPSS 17.0 for t-test, and differences were considered statistically significant when P < 0.05. The results are shown in Table 5.
TABLE 5 Effect of each group of drugs on acute Soft tissue injury in rats
Note: n is equal to 10, and n is equal to 10,*，**respectively represent comparison with blank control group P<0.05，P<0.01。
The results in table 5 show: compared with a blank control group, the acute soft tissue injury degree of the positive control group and the test group is obviously reduced, and the experimental group has better treatment effect than that of a positive control group rat, which shows that the concentrated traditional Chinese medicine composition paste in the embodiment 1 has good treatment effect on the acute soft tissue injury model rat.
Application test 5: clinical test of prevention and treatment of neck, shoulder, waist and leg pain using the hot-melt pressure-sensitive adhesive patch of the traditional Chinese medicine composition in example 3
5.1 data and methods
The hot melt pressure sensitive adhesive plaster is used for preventing and treating 100 patients with neck, shoulder, waist and leg pain, and clinical observation is carried out on 54 male patients and 46 female patients, wherein the age of the male patients is between 28 and 94 years, and the average age of the female patients is 46 years.
5.2 the diagnosis standard meets the middle cold-dampness type related diagnosis standard of the Chinese clinical disease diagnosis curative effect standard, and the patients show that the neck, shoulder, waist and leg are acid and swollen, pain, turn to the side is unfavorable, the patients feel cold and aggravate, the limbs are cold and the like.
5.3 inclusion and exclusion standard inclusion standard, namely, meeting the diagnosis standard; secondly, the consciousness is clear, and the communication can be carried out automatically; voluntarily participating and signing an informed consent. The elimination standard is that the liver, kidney, heart and lung are combined to have abnormal functions or organic lesions; ② there is operation history of neck, shoulder, waist and leg; ③ accompanied with proliferative spondylitis, spinal stenosis, intraspinal lesions and other lumbar diseases; and fourthly, the patient can not be matched with the treatment.
5.4, method: a multicenter random single-blind parallel control method is adopted, random numbers are generated by a random number table (refer to medical experiment design and statistical analysis), and a test group and a control group are regulated, wherein each group comprises 50 cases. Experimental groups: the Chinese medicinal composition hot-melt pressure-sensitive adhesive prepared in example 3 is applied to the affected part once a day for 12 hours. Control group: the appearance of the plaster is consistent with that of the plaster in the example 3, but the traditional Chinese medicine composition is not contained. The experiment was observed 3 days later.
5.5 observation indexes and judgment standards: the two groups of patients were observed continuously and the treatment effect and the basal disease pain score were recorded for both groups of patients. The clinical curative effect is that the clinical symptoms such as neck, shoulder, waist and leg pains and the like are completely disappeared, the limb movement is not limited, and the recovery is realized after the normal recovery; the clinical symptoms such as neck, shoulder, waist and leg pains and the like are obviously improved, and the limb movement is basically recovered to be obvious; the symptoms of neck, shoulder, waist and leg pain are improved, and the limb movement is improved to be effective; neither clinical symptoms nor limb movement were restored to ineffectiveness.
5.6 results
The statistical method comprises the following steps: the data processing is carried out by adopting SPSS 17.0 software, the counting data is expressed by percentage and Chi is adopted2The test shows that the difference is statistically significant when P is less than 0.05. The results are shown in Table 6.
TABLE 6 statistics of clinical effects
As can be seen from Table 6, the total effective rate of the treatment in the experimental group was higher than that in the control group, and the difference was statistically significant (P < 0.05).
Claims (5)
1. A traditional Chinese medicine composition for diminishing inflammation, relieving pain or resisting bacteria is characterized by mainly comprising the following raw material medicines in parts by weight: 60-120 parts of dried ginger, 20-40 parts of turmeric, 20-40 parts of pepper, 20-40 parts of ramulus et folium Claoxyli, 20-40 parts of wormwood, 20-40 parts of rhizoma Gastrodiae, 5-20 parts of dried orange peel, 5-20 parts of green tangerine peel, 5-20 parts of notopterygium root, 5-20 parts of radix Clematidis, 5-20 parts of radix Linderae, 5-20 parts of cortex Phellodendri, 5-20 parts of radix Dipsaci, 5-20 parts of radix Paeoniae Rubra, 5-20 parts of licorice, 5-20 parts of Angelica sinensis, 5-20 parts of Ligusticum wallichii, 5-20 parts of radix Ampelopsis, 5-20 parts of asarum, 5-20 parts of raw fructus Aurantii, 5-20 parts of chaulmoogra seed, 5-20 parts of raw eucommia bark, 5-20 parts of raw radix Aconiti lateralis, 5-20 parts of costus root, 5-20 parts of raw Bombyx Batryticatus, 5-20 parts of raw fennel, 5, 5-20 parts of raw peach kernels, 5-20 parts of raw polygala tenuifolia, 5-20 parts of garden balsam stems, 5-20 parts of caulis sinomenii, 5-20 parts of common cnidium fruits, 5-20 parts of radix achyranthis bidentatae, 5-20 parts of Chinese dodder seeds, 5-20 parts of raw rhizoma cyperi, 5-20 parts of raw bighead atractylodes rhizome, 5-20 parts of raw szechwan chinaberry fruits, 5-20 parts of cinnamon, 5-20 parts of camphor, 5-20 parts of dragon's blood, 5-20 parts of catechu, 5-20 parts of myrrh, 5-20 parts of frankincense and 5-20 parts of flos caryophylli.
2. The method for preparing a Chinese medicinal composition with antiinflammatory, analgesic, and antibacterial effects as claimed in claim 1, wherein the raw materials except cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum, and flos Caryophylli are proportionally taken and placed in an extraction tank, and are extracted by heating and refluxing with 70-80% ethanol, and the extractive solution is filtered and concentrated under reduced pressure to relative density of 1.4-1.6; adding cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli powder, mixing, and concentrating to obtain Chinese medicinal composition paste.
3. The method for preparing a Chinese medicinal composition for anti-inflammatory, analgesic or antibacterial of claim 2, wherein the other raw materials except for cinnamon, camphor, dragon's blood, catechu, myrrh, frankincense and cloves are mixed with 70-80% ethanol in a solid-to-liquid ratio of 1 g: adding 5-8 ml; reflux extracting for 2-4 times, each for 2-4 hr.
4. The method for preparing the anti-inflammatory, analgesic or antibacterial Chinese medicinal composition of claim 1, comprising the steps of:
1) taking the other raw materials except cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli at a certain proportion, placing in an extraction tank, extracting with 70-80% ethanol under heating and refluxing, filtering the extractive solution, and concentrating under reduced pressure to relative density of 1.4-1.6 to obtain paste for use;
2) melting 1000 parts of hot melt pressure sensitive adhesive at 300 ℃, cooling to 175 ℃, adding the paste, and cinnamon, camphor, dragon's blood, catechu, myrrh, frankincense and flos caryophyllata powder, and uniformly mixing; and coating the fabric with a coating machine, wherein the coating thickness is 0.5mm, the surface is covered with ion paper, and the fabric is cut into patches with the length and the width of 8 x 12cm, namely the hot melt pressure sensitive adhesive patches.
5. The method for preparing the anti-inflammatory, analgesic or antibacterial Chinese medicinal composition of claim 1, comprising the steps of:
1) proportionally placing the other raw materials except cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli in an extraction tank, extracting with 70-80% ethanol under heating and refluxing, filtering the extractive solution, and concentrating under reduced pressure to relative density of 1.4-1.6; adding cortex Cinnamomi, Camphora, sanguis Draxonis, Catechu, Myrrha, Olibanum and flos Caryophylli powder, mixing to obtain concentrated unguent of Chinese medicinal composition;
2) weighing 20 parts of the traditional Chinese medicine composition concentrated paste, 10 parts of anhydrous lanolin and 100 parts of vaseline for later use;
3) placing the concentrated Chinese medicinal composition paste into a mortar, adding sterilized vaseline and anhydrous lanolin cooled to 55-65 deg.C, and grinding.
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| CN116077400A (en) * | 2021-11-08 | 2023-05-09 | 厦门本草真源生物医药科技有限公司 | Plant formula with antioxidant and anti-aging effects and application thereof |
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| CN1915375A (en) * | 2006-08-10 | 2007-02-21 | 沈阳东陵药业股份有限公司 | Chinese traditional medicine for expeling wind and cold, relaxing muscles and tendons, harmonizing link and alleviating pain, and method for fabricating dog skin plaster purpose made |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN1915375A (en) * | 2006-08-10 | 2007-02-21 | 沈阳东陵药业股份有限公司 | Chinese traditional medicine for expeling wind and cold, relaxing muscles and tendons, harmonizing link and alleviating pain, and method for fabricating dog skin plaster purpose made |
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| 陈惠藩: "《中医外科常用外用方选》", 31 August 1986, 福建科学技术出版社 * |
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| CN116077400A (en) * | 2021-11-08 | 2023-05-09 | 厦门本草真源生物医药科技有限公司 | Plant formula with antioxidant and anti-aging effects and application thereof |
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