CN116076191A

CN116076191A - Method for improving physical and chemical properties of soil by using leymus chinensis

Info

Publication number: CN116076191A
Application number: CN202310092825.XA
Authority: CN
Inventors: 林宇龙; 梅琳琳; 崔国文; 李冰; 张攀; 秦立刚; 胡国富; 王明君
Original assignee: Northeast Agricultural University
Current assignee: Northeast Agricultural University
Priority date: 2023-02-09
Filing date: 2023-02-09
Publication date: 2023-05-09
Anticipated expiration: 2043-02-09
Also published as: CN116076191B

Abstract

The invention discloses a method for improving physical and chemical properties of soil by using leymus chinensis, belonging to the technical field of agricultural planting, which comprises the following steps: (1) seed pretreatment: removing glume of the leymus chinensis seeds, sterilizing the seeds, and putting the sterilized seeds into polyethylene glycol solution and an accelerant for pretreatment to obtain an accelerant-seed mixed solution; (2) seed germination treatment: mixing the promoter-seed mixed solution into a matrix, spreading to 0.5-1cm thick, and culturing to obtain germinated seeds; (3) sowing: mixing germinated seeds with matrix at a ratio of 1-1.5kg/m ² Is planted in the saline-alkali soil to treat the saline-alkali soil. According to the invention, the problem of strong dormancy and low germination rate of the leymus chinensis seeds is solved by treating the leymus chinensis seeds, so that the germination and growth of the leymus chinensis are promoted, and the improvement effect of the leymus chinensis on the physical and chemical properties of the saline-alkali soil is further improved.

Description

Method for improving physical and chemical properties of soil by using leymus chinensis

Technical Field

The invention relates to the technical field of agricultural planting, in particular to a method for improving physical and chemical properties of soil by using leymus chinensis.

Background

Soil salinization is a worldwide problem of soil degradation and has become one of the major limiting factors impeding global agricultural production. The salinization land area of China is about 1 hundred million hm & lt 2 & gt, and continuously grows at a very high rate every year, 50% of cultivated land is expected to face salinization trend in 2050, the land capacity is seriously hindered from being lifted, grain safety and land resource reasonable utilization are threatened, and serious economic and ecological problems are caused. In recent years, phytoremediation has been increasingly used in saline-alkali soil remediation with its excellent ecological and economic value. Rhizosphere is a tiny soil area around the plant root, which is the basis for the soil to form a carbon cycle and the ultimate productivity of the terrestrial ecosystem of the earth. The root secretion is active with the rhizosphere microorganism interaction system in the rhizosphere ecological environment, and plays an important role in the plant restoration process.

Leymus chinensis (Trin.) Tzvel is a perennial grass, a dominant species of northeast pine tender grassland plant community, has strong cold resistance and salt and alkali resistance and high grass yield, and is a high-grade grass which is not available in animal husbandry, especially dairy cow breeding. The root secretion of the leymus chinensis can improve the physical and chemical properties and the structure of the soil so as to repair degraded lands and saline-alkali lands, the root secretion of the leymus chinensis can increase the organic matters, total nitrogen, quick-acting phosphorus and other soil nutrients of the soil, the conductivity, the pH and the like of the soil are reduced so as to repair the degraded lands and the saline-alkali lands, and the soil is repaired by planting the leymus chinensis, so that the leymus chinensis is an important measure for soil repair. However, the leymus chinensis seeds have strong dormancy characteristics, and the germination rate of the seeds is low, so that the soil restoration effect is greatly reduced.

Therefore, a method for improving the physical and chemical properties of soil by using the leymus chinensis is needed at present, and the problem that the soil restoration effect is low due to high dormancy and low germination rate of leymus chinensis seeds is solved.

Disclosure of Invention

In view of the above, the invention aims to provide a method for improving physical and chemical properties of soil by using leymus chinensis, which solves the problems of high dormancy and low germination rate of leymus chinensis seeds, and further solves the problem of low soil restoration efficiency of leymus chinensis.

The invention solves the technical problems by the following technical means:

a method for improving physical and chemical properties of soil by using leymus chinensis, which comprises the following steps:

(1) Seed pretreatment: removing glume of the leymus chinensis seeds, sterilizing the seeds, and putting the sterilized seeds into polyethylene glycol solution and an accelerant for pretreatment to obtain an accelerant-seed mixed solution;

(2) Seed germination treatment: mixing the promoter-seed mixed solution into matrix, and then spreading with thickness of 0.5-1cm for cultivation to obtain germinated seeds;

(3) Sowing: mixing germinated seeds with matrix at a ratio of 1-1.5kg/m ² Is planted in the saline-alkali soil to treat the saline-alkali soil.

Further, the seed glume removal method in step (1) is as follows:

soaking the seeds in 50-70% sulfuric acid solution for 8-15 mm, rinsing with clear water, kneading for 5-8min, removing glume, and air drying to obtain shelled seeds.

The glume on the surface of the leymus chinensis seeds is an important factor for inhibiting the germination of the leymus chinensis seeds, and the glume of the leymus chinensis seeds is removed by rubbing by hands after being soaked in sulfuric acid, so that the inhibition effect of the glume on the germination of the seeds is eliminated, and the germination rate of the leymus chinensis seeds can be effectively improved.

Further, the seed disinfection method in the step (1) is as follows:

soaking dehulled seeds in 2-5% sodium hypochlorite solution for 5-8min, and washing with sterile water for 3-5 times to obtain sterilized seeds.

Further, the seed pretreatment method in the step (1) is as follows:

soaking the sterilized seeds in 2-5% polyethylene glycol solution for 15-25 hr, and soaking the seeds in the promoter for 20-36 hr to obtain promoter-seed mixed solution.

The polyethylene glycol is adopted to treat the seeds, so that the water absorption rate of the leymus chinensis seeds during germination can be slowed down, the damage of a cell membrane system caused by cell swelling due to the fact that the water absorption rate is too high is prevented, in addition, the hormone content, the protein content and the metabolism of the seeds in the germination process can be regulated through the polyethylene glycol treatment, and the germination of the seeds is promoted.

Further, the seed cultivation method in the step (2) is as follows:

sterilizing the substrate, spraying water to soak the substrate, adding the accelerator-seed mixed solution into the substrate, uniformly mixing, spreading the mixture to a thickness of 0.5-1cm, culturing at 25 ℃ for 16 hours, culturing at 20 ℃ for 8 hours, circulating with 24 hours as a period, and pouring water every 12 hours; and excavating alkaline soil with half of the weight of the matrix after 5 days, uniformly mixing the seeds, the matrix and the alkaline soil to a thickness of 0.5-1cm, continuously culturing, adding the alkaline soil every 5 days according to the same method, and obtaining the germinated seeds after 10-20 days.

After the Chinese caterpillar fungus is sowed in the saline-alkali soil, the saline-alkali soil can have adverse effect on the germination and growth of the Chinese caterpillar fungus, so that root hair cells are dehydrated, cell poisoning and the like.

Further, the matrix is vermiculite, perlite and carbonized rice husk according to the weight ratio of 1:1:1, and mixing the materials in proportion.

Further, the weight ratio of the matrix to the seeds is 100: (2-5).

Further, the accelerator comprises the following raw materials in parts by weight:

5-7 parts of sodium sulfanilate, 5-7 parts of N-ethylaniline, 3-5 parts of ammonium persulfate, 3-5 parts of L-cysteine, 5-10 parts of vitamin B1 and 5-10 parts of papain.

Further, the preparation method of the accelerator comprises the following steps:

(1) Adding water into sodium sulfanilate, and stirring until the sodium sulfanilate is dissolved to obtain a sodium sulfanilate solution; adding 75wt% ethanol into N-ethylaniline, stirring uniformly, adding a sodium sulfanilate solution, and stirring at the speed of 200-300r/min for 2-3min to obtain a mixed solution A;

(2) Mixing ammonium persulfate and L-cysteine, and then adding the mixture into water for dissolution to obtain a mixed solution B;

(3) Mixing vitamin B1 and papain, adding into water, stirring for dissolving, adding mixed solution A and mixed solution B, and stirring at 500-1000r/min for 8-10min to obtain the final product.

Further, the weight ratio of the accelerator to the seeds is (20-30): 1.

the method is characterized in that the method comprises the steps of planting the leymus chinensis in the saline-alkali soil, enabling a leymus chinensis root secretion to be active with a rhizosphere microorganism interaction system in a rhizosphere ecological environment, improving physical and chemical properties of the saline-alkali soil and further repairing the saline-alkali soil, wherein the repairing effect of leymus chinensis seeds is reduced due to low growth condition after the leymus chinensis is directly planted due to strong dormancy and low germination rate, the ideal effect of the leymus chinensis cannot be achieved due to the fact that the germination rate of the leymus chinensis seeds is improved after shelling treatment and polyethylene glycol treatment, and the germination rate of the leymus chinensis seeds can be further improved by adding an accelerant, so that the repairing effect is improved due to the fact that seeds are treated by the aid of the accelerant, metabolic pathways of the leymus chinensis seeds in the germination process can be changed, dormancy of the leymus chinensis seeds is broken, and germination of the leymus chinensis seeds is further promoted; ammonium persulfate in the accelerator enters the inside of the seed to promote the decomposition of abscisic acid in the seed, so that the inhibition effect of the abscisic acid on seed germination is reduced; l-cysteine in the promoter can be combined with abscisic acid after entering the inside of the seeds to deactivate the abscisic acid, so that the inhibition effect of the abscisic acid on seed germination is further reduced; vitamin B1 and papain in the accelerator enter the interior of the seeds, so that fat in the seeds is promoted to be converted into acetyl coenzyme A, gibberellin is generated under the internal reaction of the acetyl coenzyme A, the content of gibberellin in the seeds is increased, the germination of the seeds is promoted, the germination rate of the seeds of the leymus chinensis is further increased through the synergistic effect of substances in the accelerator, and the problem that the repair effect is low due to the fact that the dormancy of the seeds of the leymus chinensis is strong and the germination rate is low is solved.

The beneficial effects are that:

1. according to the method for improving the physicochemical properties of the soil by using the leymus chinensis, disclosed by the invention, organic matters, total nitrogen, quick-acting phosphorus and other soil nutrients of the soil can be increased by planting the leymus chinensis in the saline-alkali soil, the conductivity, pH and the like of the soil are reduced, the physicochemical properties of the saline-alkali soil can be effectively improved, and the degraded soil and the saline-alkali soil can be further repaired.

2. According to the method for improving the physical and chemical properties of the soil by using the leymus chinensis, disclosed by the invention, the problems of strong dormancy and low germination rate of leymus chinensis seeds are solved by treating the leymus chinensis seeds, so that the germination rate of the leymus chinensis seeds is improved, and the restoration effect of saline-alkali soil is further improved.

Drawings

Fig. 1: the growth condition diagram of the leymus chinensis in the experimental group 1 is shown in the invention;

fig. 2: is a detailed diagram of the growth condition of the leymus chinensis in the experimental group 1.

Detailed Description

The invention will be described in detail below with reference to specific embodiments and accompanying drawings:

the invention provides a method for improving the physical and chemical properties of soil by using leymus chinensis, which comprises the following steps of:

example 1: preparation of the promoter

600g of sodium sulfanilate, 600g N-ethylaniline, 400g of ammonium persulfate, 400g L-cysteine, 700g of vitamin B1 and 700g of papain are weighed.

The preparation method comprises the following steps:

(1) Adding 6kg of water into sodium sulfanilate, and stirring until the sodium sulfanilate is dissolved to obtain a sodium sulfanilate solution; adding 3kg of 75wt% ethanol into N-ethylaniline, uniformly stirring, adding a sodium sulfanilate solution, and stirring at the speed of 250r/min for 2min to obtain a mixed solution A;

(2) Mixing ammonium persulfate and L-cysteine, and then adding the mixture into 8kg of water for dissolution to obtain a mixed solution B;

(3) Mixing vitamin B1 and papain, adding into 14kg water, stirring for dissolving, adding the mixed solution A and the mixed solution B, and stirring at 700r/min for 9min to obtain the promoter.

Example 2: preparation of accelerator II

500g of sodium sulfanilate, 500g N-ethylaniline, 300g of ammonium persulfate, 300g L-cysteine, 500g of vitamin B1 and 500g of papain are weighed.

The preparation method comprises the following steps:

(1) Adding 5kg of water into sodium sulfanilate, and stirring until the sodium sulfanilate is dissolved to obtain a sodium sulfanilate solution; adding 2.5kg of 75wt% ethanol into N-ethylaniline, uniformly stirring, adding a sodium sulfanilate solution, and stirring at the speed of 200r/min for 2min to obtain a mixed solution A;

(2) Mixing ammonium persulfate and L-cysteine, and then adding the mixture into 6kg of water for dissolution to obtain a mixed solution B;

(3) Mixing vitamin B1 and papain, adding into 10kg of water, dissolving, adding the mixed solution A and the mixed solution B, and stirring at 500r/min for 8min to obtain the promoter.

Example 3: preparation of accelerator three

700g of sodium sulfanilate, 700g N-ethylaniline, 500g of ammonium persulfate, 500g L-cysteine, 1kg of vitamin B1 and 1kg of papain are weighed.

The preparation method comprises the following steps:

(1) Adding 7kg of water into sodium sulfanilate, and stirring until the sodium sulfanilate is dissolved to obtain a sodium sulfanilate solution; adding 3.5kg of 75wt% ethanol into N-ethylaniline, uniformly stirring, adding a sodium sulfanilate solution, and stirring at the speed of 300r/min for 3min to obtain a mixed solution A;

(2) Mixing ammonium persulfate and L-cysteine, and then adding into 10kg of water to dissolve to obtain a mixed solution B;

(3) Mixing vitamin B1 and papain, adding into 20kg of water, stirring for dissolving, adding the mixed solution A and the mixed solution B, and stirring at 1000r/min for 10min to obtain the promoter.

Comparative example 1: preparation of promoter

A comparative example was formed with example 1, except that the accelerator was prepared without adding the mixed solution A prepared from sodium sulfanilic acid and N-ethylaniline.

400g of ammonium persulfate, 400g L-cysteine, 700g of vitamin B1 and 700g of papain were weighed out.

The preparation method comprises the following steps:

(1) Mixing ammonium persulfate and L-cysteine, and then adding the mixture into 8kg of water for dissolution to obtain a mixed solution B;

(2) Mixing vitamin B1 and papain, adding into 14kg water, stirring for dissolving, adding the mixed solution B, and stirring at 700r/min for 9min to obtain the final product.

Comparative example 2: preparation of promoter

A comparative example was formed with example 1, except that no ammonium persulfate was added during the accelerator preparation.

500g of sodium sulfanilic acid, 500g N-ethylaniline, 400g L-cysteine, 500g of vitamin B1 and 500g of papain are weighed.

The preparation method comprises the following steps:

(2) Adding 4kg of water into L-cysteine for dissolving to obtain a mixed solution B;

Comparative example 3: preparation of promoter

A comparative example was formed with example 1, except that the accelerator was prepared without L-cysteine.

The preparation method comprises the following steps:

(2) Adding 4kg of water into ammonium persulfate to dissolve to obtain a mixed solution B;

Comparative example 4: preparation of promoter

In contrast to example 1, the exception is that the accelerator was prepared without vitamin B1 and papain.

The preparation method comprises the following steps:

(3) Mixing the mixed solution A and the mixed solution B, and stirring at the speed of 700r/min for 9min to obtain the accelerator.

Example 4: method for improving physical and chemical properties of soil by using leymus chinensis

Example 4 the accelerator prepared in example 1 was used.

(1) Seed pretreatment: soaking 100g of seeds in a sulfuric acid solution with the mass concentration of 60% for 12 mm, washing with clear water, rubbing for 6min by hand, then putting into water, fishing out glume, fishing out the seeds, and air-drying to obtain shelled seeds; soaking the dehulled seeds in a sodium hypochlorite solution with the mass concentration of 3% for 7min, and then washing the seeds with sterile water for 4 times to obtain sterilized seeds; soaking the sterilized seeds in a polyethylene glycol solution with the mass concentration of 3% for 20 hours, and then taking out the seeds, soaking the seeds in 2.5kg of the accelerator prepared in the example 1 for 30 hours to obtain an accelerator-seed mixed solution;

(2) Seed germination treatment: respectively weighing 833g of vermiculite, perlite and carbonized rice husk, mixing, and stir-frying in an iron pan at 120 ℃ for 10min to obtain a sterilized matrix; spraying water on the sterilized substrate to fully soak the substrate, adding the accelerator-seed mixed solution into the substrate, uniformly mixing, spreading the mixture to the thickness of 0.6cm, culturing the mixture at 25 ℃ for 16 hours, culturing the mixture at 20 ℃ for 8 hours, circulating the mixture for 24 hours, and pouring water once every 12 hours to fully soak the substrate; digging 1.25kg of saline-alkali soil after 5 days, uniformly mixing seeds, matrixes and saline-alkali soil, spreading the mixture to a thickness of 0.6cm, continuously culturing, adding the saline-alkali soil every 5 days according to the same method, and obtaining germinated seeds after 15 days;

(3) Sowing: the germinated seeds were mixed with the matrix soil at 1.3kg/m ² Is planted in the saline-alkali soil to treat the saline-alkali soil.

Comparative example 5: method for improving physical and chemical properties of soil by using leymus chinensis

A comparative example was formed with example 4, except that no dehulling treatment was performed at the time of seed pretreatment.

(1) Seed pretreatment: soaking 100g of seeds in a sodium hypochlorite solution with the mass concentration of 3% for 7min, and then washing with sterile water for 4 times to obtain sterilized seeds; soaking the sterilized seeds in a polyethylene glycol solution with the mass concentration of 3% for 20 hours, and then taking out the seeds, soaking the seeds in 2.5kg of the accelerator prepared in the example 1 for 30 hours to obtain an accelerator-seed mixed solution;

Comparative example 6: method for improving physical and chemical properties of soil by using leymus chinensis

A comparative example was formed with example 4, except that seed pretreatment was not performed with polyethylene glycol solution.

(1) Seed pretreatment: soaking 100g of seeds in a sulfuric acid solution with the mass concentration of 60% for 12 mm, washing with clear water, rubbing for 6min by hand, putting into water, fishing out glume, fishing out the seeds, and air-drying to obtain shelled seeds; soaking the dehulled seeds in a sodium hypochlorite solution with the mass concentration of 3% for 7min, and then washing the seeds with sterile water for 4 times to obtain sterilized seeds; soaking the sterilized seeds in 2.5kg of the promoter prepared in example 1 for 30 hours to obtain a promoter-seed mixed solution;

(2) Seed germination treatment: respectively weighing 833g of vermiculite, perlite and carbonized rice husk, mixing, and stir-frying in an iron pan at 120 ℃ for 10min to obtain a sterilized matrix; spraying water on the sterilized substrate to fully soak the substrate, adding the accelerator-seed mixed solution into the substrate, uniformly mixing, spreading the mixture to the thickness of 0.6cm, culturing the mixture at 25 ℃ for 16 hours, culturing the mixture at 20 ℃ for 8 hours, circulating the mixture for 24 hours, and pouring water once every 12 hours to fully soak the substrate; digging 1.25kg of saline-alkali soil after 5 days, uniformly mixing seeds, matrixes and saline-alkali soil to a thickness of 0.6cm, continuously culturing, adding the saline-alkali soil every 5 days according to the same method, and obtaining germinated seeds after 15 days;

Comparative example 7: method for improving physical and chemical properties of soil by using leymus chinensis

In contrast to example 4, the only difference is that the seed pretreatment is not carried out with the accelerator.

(1) Seed pretreatment: soaking 100g of seeds in a sulfuric acid solution with the mass concentration of 60% for 12 mm, washing with clear water, rubbing for 6min by hand, putting into water, fishing out glume, fishing out the seeds, and air-drying to obtain shelled seeds; soaking the dehulled seeds in a sodium hypochlorite solution with the mass concentration of 3% for 7min, and then washing the seeds with sterile water for 4 times to obtain sterilized seeds; soaking the sterilized seeds in a polyethylene glycol solution with the mass concentration of 3% for 20 hours, and then fishing out the seeds to obtain pretreated seeds;

(2) Seed germination treatment: respectively weighing 833g of vermiculite, perlite and carbonized rice husk, mixing, and stir-frying in an iron pan at 120 ℃ for 10min to obtain a sterilized matrix; spraying water on the sterilized substrate to fully soak the substrate, adding the pretreated seeds into the substrate, uniformly mixing, spreading the seeds to the thickness of 0.6cm, culturing the seeds at 25 ℃ for 16 hours, culturing the seeds at 20 ℃ for 8 hours, circulating the seeds with the period of 24 hours, and pouring water once every 12 hours to fully soak the substrate; digging 1.25kg of saline-alkali soil after 5 days, uniformly mixing seeds, matrixes and saline-alkali soil to a thickness of 0.6cm, continuously culturing, adding the saline-alkali soil every 5 days according to the same method, and obtaining germinated seeds after 15 days;

Comparative example 8: method for improving physical and chemical properties of soil by using leymus chinensis

In contrast to example 4, the only difference is that saline-alkali soil is not added to the substrate at the time of seed germination treatment, but normal soil is added.

(1) Seed pretreatment: soaking 100g of seeds in a sulfuric acid solution with the mass concentration of 60% for 12 mm, washing with clear water, rubbing for 6min by hand, putting into water, fishing out glume, fishing out the seeds, and air-drying to obtain shelled seeds; soaking the dehulled seeds in a sodium hypochlorite solution with the mass concentration of 3% for 7min, and then washing the seeds with sterile water for 4 times to obtain sterilized seeds; soaking the sterilized seeds in a polyethylene glycol solution with the mass concentration of 3% for 20 hours, and then taking out the seeds, soaking the seeds in 2.5kg of the accelerator prepared in the example 1 for 30 hours to obtain an accelerator-seed mixed solution;

(2) Seed germination treatment: respectively weighing and mixing vermiculite, perlite and carbonized rice husk 833g, and putting the mixture into an iron pan for stir-frying at 120 ℃ for 10min to obtain a sterilized matrix; spraying water on the sterilized substrate to fully soak the substrate, adding an accelerator-seed mixed solution into the substrate, uniformly mixing, spreading the substrate to a thickness of 0.6cm, culturing the substrate for 16 hours at 25 ℃, culturing the substrate for 8 hours at 20 ℃, circulating the substrate with a period of 24 hours, pouring water once every 12 hours to fully soak the substrate, digging 1.25kg of normal soil after 5 days, uniformly mixing the seed, the substrate and saline-alkali soil to a thickness of 0.6cm, continuously culturing the substrate, adding the normal soil once every 5 days according to the same method, and obtaining germinated seeds after 15 days;

Blank control group:

the blank control group seeds are not dehulled and treated by polyethylene glycol solution, are directly disinfected, are soaked by adopting clear water as an accelerator and are pretreated, and saline-alkali soil is not added during germination treatment, but normal soil is added.

(1) Seed pretreatment: soaking 100g of seeds in a sodium hypochlorite solution with the mass concentration of 3% for 7min for sterilization, and then washing with sterile water for 4 times to obtain sterilized seeds; soaking the sterilized seeds in 2.5kg of clear water for 30 hours to obtain a clear water-seed mixed solution;

(2) Seed germination treatment: respectively weighing and mixing vermiculite, perlite and carbonized rice husk 833g, and putting the mixture into an iron pan for stir-frying at 120 ℃ for 10min to obtain a sterilized matrix; spraying water on the sterilized substrate to fully soak the substrate, adding clear water-seed mixed solution into the substrate, uniformly mixing, spreading the substrate to the thickness of 0.6cm, culturing the substrate for 16 hours at 25 ℃, culturing the substrate for 8 hours at 20 ℃, circulating the substrate with 24 hours as a period, pouring water once every 12 hours to fully soak the substrate, digging 1.25kg of normal soil after 5 days, uniformly mixing the seed, the substrate and saline-alkali soil to the thickness of 0.6cm, continuously culturing the substrate, adding the normal soil once every 5 days according to the same method, and obtaining germinated seeds after 15 days;

Experiment: experiment for improving physical and chemical properties of soil by using leymus chinensis

1. For better comparison, the accelerators prepared in example 1 and comparative examples 1 to 4 were subjected to experiments for improving physical and chemical properties of soil by leymus chinensis, and the experiments were divided into 10 groups: experimental group 1, control groups 1-8, and blank control group. Selecting an demonstration base for restoring and treating grasslands of northeast agricultural university to perform experiments for improving physical and chemical properties of the soil of the leymus chinensis, dividing a region as an experimental area and measuringThe initial physicochemical parameters (organic matter, total nitrogen, quick-acting phosphorus, conductivity and pH) of the soil in the experimental area are shown in Table 1, and the soil in the experimental area is divided into 10 areas of 16m after being ploughed and raked uniformly ² The square small areas of the formula (1) correspond to the experimental group (1), the control group (1-8) and the blank control group respectively, and then the Chinese wildrye is planted to improve the physical and chemical properties of the soil.

2. Accelerating agent

Experiment group 1: the accelerator prepared in example 1 was selected;

control groups 1-4: the accelerators prepared in comparative examples 1 to 4 were selected, respectively;

control group 5-8: the accelerator prepared in example 1 was selected;

blank control group: clean water was selected as a control.

3. Method for improving physical and chemical properties of soil by using leymus chinensis

Experiment group 1: a method for improving physical and chemical properties of soil by using the leymus chinensis in the embodiment 4 is selected;

control groups 1-4: a method for improving physical and chemical properties of soil by using the leymus chinensis in the embodiment 4 is selected;

control group 5-8: the method for improving the physical and chemical properties of the soil by using the leymus chinensis of the comparative examples 5-8 is respectively selected;

blank control group: a method for improving physical and chemical properties of soil by selecting herba Caprae Seu Ovis in blank control group is provided.

4. And (3) germination percentage measurement: after 15 days of seed germination treatment, 100g of a seed matrix soil mixture was randomly selected from the experimental group 1, the control groups 1 to 8 and the blank control group, the seeds in the matrix soil mixture were sorted out and separated into germinated seeds and ungerminated seeds, the seed germination rate was calculated, and 3 replicates were performed, and average data were obtained as shown in table 2.

5. Improvement effect measurement: the physical and chemical parameters of each group of soil were measured again after one year of the cultivation of leymus chinensis, and the obtained data are shown in table 3.

TABLE 1

Organic matter (g/kg)	Total nitrogen (g/kg)	Quick-acting phosphorus (mg/kg)	Conductivity (ms/cm)	pH
					8.74	0.56	5.38	0.45	8.37

TABLE 2

TABLE 3 Table 3

From the data analysis of table 2, it can be seen that:

(1) Compared with the experimental group 1, the germination rate of the seeds of the experimental group 1 is obviously higher than that of the experimental group 1-8 and the blank control group, wherein the germination rate of the seeds of the experimental group 1 is 9.9 percent higher than that of the experimental group 1, and the control group 1 is prepared by the promoter without adding the mixed solution A prepared from sodium sulfanilate and N-ethylaniline, so that the metabolic pathway of the seeds is not changed, the dormancy of the seeds is broken, and the germination rate of the seeds is reduced;

(2) Compared with the experimental group 1, the germination rate of the seeds in the control group 2 is 11 percent higher, because the accelerator of the control group 2 is not added with ammonium persulfate when being prepared, and can not promote the decomposition of the abscisic acid, the abscisic acid can inhibit the germination of the seeds, and the germination rate of the seeds is reduced;

(3) Compared with the experimental group 1, the germination rate of the seeds in the control group 3 is 10 percent higher than that in the experimental group 1, because the accelerator of the control group 3 is not added with L-cysteine during preparation, and cannot be combined with the abscisic acid to inactivate the abscisic acid, the abscisic acid inhibits the germination of the seeds, and the germination rate of the seeds is reduced;

(4) Compared with the experimental group 1, the germination rate of the seeds in the control group 4 is higher than 11.1%, because the promoter in the control group 4 is not added with vitamin B1 and papain during preparation, the fat in the seeds cannot be promoted to be converted into acetyl coenzyme A, so that the gibberellin content in the seeds cannot be increased, the germination of the seeds cannot be promoted, and the germination rate of the seeds is reduced;

(5) Compared with the experimental group 1, the germination rate of the seeds in the experimental group 1 is 16.1 percent higher in the control group 5, because the control group 5 is not dehulled when being subjected to the seed pretreatment, the seed shell inhibits the germination of the seeds, and the germination rate of the seeds is reduced;

(6) Compared with the experimental group 1, the germination rate of the seeds in the control group 6 is 15.7 percent higher than that in the experimental group 1, because the control group 6 is not treated by polyethylene glycol solution when the seed pretreatment is carried out, the hormone content, the protein content and the metabolism of the seeds cannot be carried out, and the germination rate of the seeds is reduced;

(7) Compared with the experimental group 1, the germination rate of the seeds in the control group 7 is 19.5% higher than that in the experimental group 1, because the control group 7 is not treated by the accelerator when being subjected to the seed pretreatment, the dormancy period of the seeds is not broken, the abscisic acid inhibition effect is reduced, the gibberellin content is improved, and the germination rate of the seeds is reduced;

(8) From the data analysis of tables 1 and 3, it can be seen that: the content of organic matters, total nitrogen and quick-acting phosphorus in the soil after the leymus chinensis is repaired is obviously increased, the conductivity and the pH value are obviously reduced, and the leymus chinensis has good repairing effect on the saline-alkali soil; compared with the control group 1-8 and the blank control group, the organic matters, total nitrogen and quick-acting phosphorus in the soil after the repair of the experimental group 1 are higher than those of the control group 1-8 and the blank control group, and the conductivity and the pH are lower than those of the control group 1-8 and the blank control group, so that the method is adopted to treat the leymus chinensis seeds and then sow the leymus seeds in the saline-alkali soil, the germination rate of the leymus seeds can be improved, the growth of leymus seeds is promoted, the physicochemical properties of the leymus soil are improved, the organic matters, total nitrogen and quick-acting phosphorus content in the saline-alkali soil are effectively increased, and the conductivity, the pH and the like of the soil are reduced.

The above embodiments are only for illustrating the technical solution of the present invention and not for limiting the same, and although the present invention has been described in detail with reference to the preferred embodiments, it should be understood by those skilled in the art that modifications and equivalents may be made thereto without departing from the spirit and scope of the technical solution of the present invention, which is intended to be covered by the scope of the claims of the present invention. The technology, shape, and construction parts of the present invention, which are not described in detail, are known in the art.

Claims

1. A method for improving physical and chemical properties of soil by using leymus chinensis, which is characterized by comprising the following steps:

(2) Seed germination treatment: mixing the promoter-seed mixed solution into a matrix, spreading to 0.5-1cm thick, and culturing to obtain germinated seeds;

2. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 1, wherein the glume removing method in the step (1) is as follows:

soaking the seeds in a sulfuric acid solution with the mass concentration of 50-70% for 8-15 mm, washing with clear water, rubbing with hands for 5-8min, putting into water, fishing out the glume, fishing out the seeds, and air-drying to obtain shelled seeds.

3. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 1, wherein the seed disinfection method in the step (1) is as follows:

4. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 1, wherein the seed pretreatment method in the step (1) is as follows:

5. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 1, wherein the seed cultivation method in the step (2) is as follows:

sterilizing the substrate, spraying water to soak the substrate, adding the accelerator-seed mixed solution into the substrate, uniformly mixing, spreading the mixture to a thickness of 0.5-1cm, culturing at 25 ℃ for 16 hours, culturing at 20 ℃ for 8 hours, circulating with 24 hours as a period, and pouring water every 12 hours; and excavating alkaline soil with a half weight of the matrix after 5 days, uniformly mixing the seeds, the matrix and the alkaline soil, spreading the mixture to a thickness of 0.5-1cm, continuously culturing, adding the alkaline soil every 5 days according to the same method, and obtaining the germinated seeds after 10-20 days.

6. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 5, wherein the matrix is vermiculite, perlite and carbonized rice husk according to a weight ratio of 1:1:1, and mixing the materials in proportion.

7. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 6, wherein the weight ratio of the matrix to the seeds is 100: (3-5).

8. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 5, wherein the accelerator comprises the following raw materials in parts by weight:

9. The method for improving physical and chemical properties of soil by using leymus chinensis according to claim 8, wherein the preparation method of the accelerator is as follows: