CN102119595B - Method for improving germination rate of triploid seedless watermelon seeds - Google Patents
Method for improving germination rate of triploid seedless watermelon seeds Download PDFInfo
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- CN102119595B CN102119595B CN2010105869000A CN201010586900A CN102119595B CN 102119595 B CN102119595 B CN 102119595B CN 2010105869000 A CN2010105869000 A CN 2010105869000A CN 201010586900 A CN201010586900 A CN 201010586900A CN 102119595 B CN102119595 B CN 102119595B
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- seed
- germination rate
- seedless watermelon
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Links
- 241000219109 Citrullus Species 0.000 title claims abstract description 34
- 235000012828 Citrullus lanatus var citroides Nutrition 0.000 title claims abstract description 34
- 238000000034 method Methods 0.000 title claims abstract description 19
- 230000035784 germination Effects 0.000 title claims abstract description 18
- 208000026487 Triploidy Diseases 0.000 title claims abstract description 17
- LHJQIRIGXXHNLA-UHFFFAOYSA-N calcium peroxide Chemical compound [Ca+2].[O-][O-] LHJQIRIGXXHNLA-UHFFFAOYSA-N 0.000 claims abstract description 21
- 239000004343 Calcium peroxide Substances 0.000 claims abstract description 12
- 235000019402 calcium peroxide Nutrition 0.000 claims abstract description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 12
- MHAJPDPJQMAIIY-UHFFFAOYSA-N Hydrogen peroxide Chemical compound OO MHAJPDPJQMAIIY-UHFFFAOYSA-N 0.000 claims abstract description 11
- 238000005406 washing Methods 0.000 claims abstract 2
- 230000007226 seed germination Effects 0.000 claims description 14
- 239000007788 liquid Substances 0.000 claims 2
- 230000000887 hydrating effect Effects 0.000 claims 1
- 238000012258 culturing Methods 0.000 abstract 1
- 239000002352 surface water Substances 0.000 abstract 1
- 239000000292 calcium oxide Substances 0.000 description 13
- ODINCKMPIJJUCX-UHFFFAOYSA-N calcium oxide Inorganic materials [Ca]=O ODINCKMPIJJUCX-UHFFFAOYSA-N 0.000 description 13
- 210000003491 skin Anatomy 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000012360 testing method Methods 0.000 description 7
- 238000005213 imbibition Methods 0.000 description 6
- 210000001161 mammalian embryo Anatomy 0.000 description 6
- 238000004519 manufacturing process Methods 0.000 description 4
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 3
- 239000012530 fluid Substances 0.000 description 3
- 239000011259 mixed solution Substances 0.000 description 3
- 239000001301 oxygen Substances 0.000 description 3
- 229910052760 oxygen Inorganic materials 0.000 description 3
- 238000004659 sterilization and disinfection Methods 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- BRPQOXSCLDDYGP-UHFFFAOYSA-N calcium oxide Chemical compound [O-2].[Ca+2] BRPQOXSCLDDYGP-UHFFFAOYSA-N 0.000 description 2
- 235000009508 confectionery Nutrition 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 235000012907 honey Nutrition 0.000 description 2
- 229960002163 hydrogen peroxide Drugs 0.000 description 2
- 238000005286 illumination Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 238000012545 processing Methods 0.000 description 2
- 239000004576 sand Substances 0.000 description 2
- 239000002904 solvent Substances 0.000 description 2
- 235000014347 soups Nutrition 0.000 description 2
- 244000241235 Citrullus lanatus Species 0.000 description 1
- 235000009831 Citrullus lanatus Nutrition 0.000 description 1
- 244000241257 Cucumis melo Species 0.000 description 1
- 235000015510 Cucumis melo subsp melo Nutrition 0.000 description 1
- 208000035240 Disease Resistance Diseases 0.000 description 1
- 208000020584 Polyploidy Diseases 0.000 description 1
- 241001062472 Stokellia anisodon Species 0.000 description 1
- 208000035199 Tetraploidy Diseases 0.000 description 1
- FJJCIZWZNKZHII-UHFFFAOYSA-N [4,6-bis(cyanoamino)-1,3,5-triazin-2-yl]cyanamide Chemical compound N#CNC1=NC(NC#N)=NC(NC#N)=N1 FJJCIZWZNKZHII-UHFFFAOYSA-N 0.000 description 1
- 238000010521 absorption reaction Methods 0.000 description 1
- 238000010923 batch production Methods 0.000 description 1
- 238000000354 decomposition reaction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 210000002615 epidermis Anatomy 0.000 description 1
- 238000011010 flushing procedure Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000007789 gas Substances 0.000 description 1
- 238000009396 hybridization Methods 0.000 description 1
- 230000036571 hydration Effects 0.000 description 1
- 238000006703 hydration reaction Methods 0.000 description 1
- 229910052739 hydrogen Inorganic materials 0.000 description 1
- 208000015181 infectious disease Diseases 0.000 description 1
- 201000004792 malaria Diseases 0.000 description 1
- 230000003211 malignant effect Effects 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 231100000252 nontoxic Toxicity 0.000 description 1
- 230000003000 nontoxic effect Effects 0.000 description 1
- 230000008520 organization Effects 0.000 description 1
- 239000007800 oxidant agent Substances 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 239000010451 perlite Substances 0.000 description 1
- 235000019362 perlite Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 239000000843 powder Substances 0.000 description 1
- 238000007781 pre-processing Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000000630 rising effect Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 238000000528 statistical test Methods 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 241001148471 unidentified anaerobic bacterium Species 0.000 description 1
- 238000005303 weighing Methods 0.000 description 1
Landscapes
- Pretreatment Of Seeds And Plants (AREA)
Abstract
The invention relates to a method for improving the germination rate of triploid seedless watermelons, which adopts the technical scheme that: treating the seeds with the pretreatment solution to swell and hydrate the seeds for 2 to 10 hours, then washing the residual solution on the seed coats with water, draining the surface water, and culturing for germination. Wherein the pretreatment solution is: contains 0.5-3% (mass/volume) of calcium peroxide and 1-5% (volume/volume) of hydrogen peroxide solution.
Description
Technical field
The present invention relates to a kind of method that improves triploid seedless watermelon seed germination rate, especially relate to a kind of through seed being carried out the method for preliminary treatment raising triploid seedless watermelon seed germination rate.
Background technology
Watermelon is important worldwide fruit, adds up calendar year 2001 according to FAO (Food and Agriculture Organization of the United Nation) (FAO), and watermelon occupies the 5th in the world's ten big fruits.1,470,000 hectares of the area under cultivations of China's watermelon, its area and gross yield all account for more than 50% of the world, can be rated as the first in the world watermelon big country.Triploid seedless watermelon [Citrullus lanatus (Thunb.) Matsum. & Nakai] has polyploid and hybrid vigour, and outstanding feature is no seed, and melon skin is thinner; Yellowish pink scarlet, sugar is higher, and the mushy water-melon pulp degree is good; Delicious taste; Strong and the anti-storage of disease resistance is welcome by broad masses deeply, the high elaboration that becomes in the watermelon of, economic worth of fine quality with article.Therefore in variety of watermelon, the shared ratio of seedless watermelon is in the trend of rising always, is seedless watermelon more than 60% in the watermelon production of developed country such as the U.S., Australia especially.But because seedless watermelon is to be obtained by tetraploid and diploid parents hybridization, the triploid seed forms difficulty relatively, and the kind amount is planted skin depth less; Hilum is hard, and embryo hypoplasia, and the absolute weight of embryo is lower; Embryo is heavy only account for seed heavy 38.5%, the embryo volume accounts for 60%～70% of kind of shell inner chamber, also has the lopsided embryo of significant proportion simultaneously; The more common watermelon of germination rate is low, therefore, how to improve the seedless watermelon seed germination rate and becomes the key technology that seedless watermelon is produced.Produce at present to go up to adopt and remove, cut or knock out kind of micromicro to improve the germination rate of seed; But this operation is easy to cause the infringement to embryo; And time-consuming, take a lot of work, the time there is sizable difficulty in large-scale seedless watermelon production in operation, the particularly domestic in recent years price of labour power is constantly soaring; Increase the seedless watermelon seedling cost, limited further developing of seedless watermelon production.
The patent of invention of Zhang Xian etc., Zheng Xiaoying etc. adopts towel and perlite and other solid matrix to cause; The method that improves triploid watermelon seed germination rate and planting percent is difficult to accomplish batch process; And the seed stock performance through causing reduces; Easy bacteria infection in the imbibition process once more after dry treatment is returned in moisture absorption is difficult to use and promote on producing.
Summary of the invention
To the deficiency of prior art, the invention provides a kind of method that improves triploid seedless watermelon seed germination rate, this method is exempted from broken shell and is handled, and simple, cost is low, and no solvent residue is fit to the mass disposal seed.
A kind of technical scheme that improves the method for triploid seedless watermelon seed germination rate provided by the invention is: adopt pretreatment fluid that seed is handled; Make its imbibition hydration 2-10 hour; Wash the residual solution on kind of the skin then, control dried surface moisture, cultivate and germinate; Wherein said pretreatment fluid is: containing the mass and size percentage concentration is that 0.5%-3% calper calcium peroxide and volume concentration expressed in percentage by volume are the solution of 1%-5% hydrogen peroxide.
Wherein pretreatment fluid is preferably: contain concentration and be 1.5% calper calcium peroxide and concentration and be 2% hydrogenperoxide steam generator.
Above-mentioned cultivation germination is meant according to national crop seeds inspection procedure germination test GB/T 3543.4 standards carries out germination test; Seed is laid in the cultivation box that clean aseptic sand is housed uniformly; Or seed is laid on the moistening towel of having sterilized, place 33 ℃ illumination box to germinate.
Calper calcium peroxide is a kind of yellowish crystalline powder, and nontoxic nothing is smelt, and has in water or slowly decomposition in the malaria, the characteristic of putting oxygen for a long time; It also can be used as oxidant and carries out disinfection, and can effectively kill anaerobic bacteria and malignant bacteria.Triploid seedless watermelon seed kind cornu cutaneum materialization, hard, seed are in the imbibition process, and having the gas permeability obstacle is the low one of the main reasons of germination rate.Use the calper calcium peroxide mixed solution to handle and to solve seed not enough problem of oxygen content in the imbibition process, reach the purpose that improves seed germination.Not only cost is low to adopt calper calcium peroxide mixed solution preliminary treatment seed, and is easy to use, and environmentally friendly.Up to the present, research and the production application of using the calper calcium peroxide mixed solution to handle seed promotion sprouting also do not arranged.
Advantage of the present invention is: simple to operate, short sprout the efficient height, and cost is low, no solvent residue, environmentally safe can the mass disposal seed, can accomplish to exempt from broken shell fully and handle, and can directly on producing, use and promote.
Embodiment
According to process of the test the present invention is further described below, but do not mean that restriction scope of the present invention.
Embodiment 1
To the preprocessing solution concentration of seed among the present invention and the screening of pretreatment time:
Observe the CaO of variable concentrations
2To the sprouting situation of triploid seedless watermelon seed treatment after 6 hours.The concentration preparation: 1% is exactly to take by weighing 1gCaO in the table 1
2Be dissolved in the 100ml water 100 seeds of 1 processing, 4 repetitions.H in the table 2
2O
2Concentration be meant at above-mentioned 1.5%CaO
2Solution in add the H of certain volume
2O
2After the volumetric concentration that obtains, like 1.5% CaO
2+ 1%H
2O
2Just be meant that liquor capacity is to add 1.5gCaO in the container of 100ml
2With 1ml H
2O
2Gained.
The CaO of table 1 variable concentrations
2Test data to triploid seedless watermelon seed germination
| CaO 2Concentration | The red no seed of honey | Black ox | Spend skin not have seed |
| 0.50% | 41.20% | 49.30% | 58.40% |
| 1% | 60.90% | 61.20% | 72.50% |
| 1.50% | 76.30% | 79.80% | 80.60% |
| 2% | 77.10% | 81% | 81.80% |
| 2.50% | 78.90% | 81.40% | 82.30% |
| 3% | 80.10% | 82.80% | 82.20% |
| Contrast (water) | 18% | 34% | 56% |
Reach a conclusion: 1.5% above concentration is not remarkable to the raising difference of germination rate, from cost consideration, therefore chooses suitable CaO
2Concentration is 1.50%.
Table 2 1.5%CaO
2H with variable concentrations
2O
2Combination is to the influence of seedless watermelon seed
| H 2O 2Concentration | The red no seed of honey | Black ox | Spend skin not have seed |
| 1% | 80.60% | 82% | 82.80% |
| 2% | 91.30% | 93.20% | 94.13 |
| 3% | 91.40% | 93% | 94.60% |
| 4% | 90.70% | 93.40% | 93.90% |
| 5% | 90.50% | 93.10% | 93.20% |
| Contrast (water) | 17.30% | 35.10% | 54.80% |
Reach a conclusion: choosing concentration is 1.5% (m/v) CaO
2H with 2% (v/v)
2O
2It is more suitable to make up.
The different pretreatment times of table 3 influence 30 ℃ of water temperatures to what close Red Indian race was sprouted
| Combined concentration | 2 hours | 4 hours | 6 hours | 8 hours | 10 hours |
| 1.5%+2% | 52.30% | 85.70% | 91.10% | 92.10% | 83.60% |
| Contrast (water) | 10.90% | 15.30% | 17.80% | 18.10% | 9.70% |
The different pretreatment times of table 4 are to the influence of black ox seed germination
| Combined concentration | 2 hours | 4 hours | 6 hours | 8 hours | 10 hours |
| 1.5%+2% | 60.10% | 87.60% | 93.50% | 92.80% | 84.50% |
| Contrast (water) | 11.30% | 26.90% | 34.60% | 32.10% | 16.90% |
The different pretreatment times of table 5 do not have the influence that seeds is sprouted to spending skin
| Combined concentration | 2 hours | 4 hours | 6 hours | 8 hours | 10 hours |
| 1.5%+2% | 62.10% | 87.90% | 94.1 | 93.50% | 79.60% |
| Contrast (water) | 14.50% | 48.40% | 56% | 55.10% | 17.80% |
Reach a conclusion: pretreatment time choose 6 hours comparatively suitable.
Embodiment 2
Experimental cultivar is the triploid seedless watermelon kind that 3 different natural are sprouted ability, is respectively sweet red no seed, Hei Niu and spends skin not have seed, and natural germination rate is respectively 18%, 34% and 56%.Select for use above-mentioned 3 variety seeds each 400, per 100 as a processing, be positioned over calper calcium peroxide (CaO simultaneously
2) and hydrogen peroxide (H
2O
2) imbibition 6 hours in the 300ml solution, calper calcium peroxide (CaO
2) and hydrogen peroxide (H
2O
2) be respectively 1.5% (m/v) and 2% (v/v), compared in 6 hours with the distilled water imbibition.Then with remaining soup on the clean seed epidermis of distilled water flushing; Control dried surface moisture; Carry out germination test according to national crop seeds inspection procedure germination test GB/T 3543.4 standards; Seed is laid in the cultivation box that clean aseptic sand is housed uniformly, or seed is laid on the moistening towel of having sterilized, place 33 ℃ illumination box to germinate.Observe the sprouting situation line item of going forward side by side after 24 hours.The statistical test result shows after 7 days: sweet red no seed germination rate after said process is handled vernalization that germination rate is lower is 91.3%, and black ox is 93.2%, and spending skin not have seed is 94.13%; And contrast is respectively 17.3%, 35.1% and 54.8%.Result of the test shows that the triploid seedless watermelon seed germination rate through containing the pretreated different germination rates of calcium oxide (CaO2) solution all is significantly improved, and adopts this solution-treated need not pass through other soup sterilizations, calper calcium peroxide (CaO
2) solution itself disinfection is arranged effect simultaneously for seed germination provides required oxygen, reach the effect that improves germination rate.
Claims (2)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105869000A CN102119595B (en) | 2010-12-14 | 2010-12-14 | Method for improving germination rate of triploid seedless watermelon seeds |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2010105869000A CN102119595B (en) | 2010-12-14 | 2010-12-14 | Method for improving germination rate of triploid seedless watermelon seeds |
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| Publication Number | Publication Date |
|---|---|
| CN102119595A CN102119595A (en) | 2011-07-13 |
| CN102119595B true CN102119595B (en) | 2012-02-15 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102498788A (en) * | 2011-11-11 | 2012-06-20 | 云南省农业科学院园艺作物研究所 | Method for improving drought resistance of pepper seedlings |
| CN107124933B (en) * | 2017-05-18 | 2020-07-07 | 安徽省农业科学院园艺研究所 | Seed-free watermelon seed-breaking-free batch seeding and seedling raising method |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49117244A (en) | 1973-03-23 | 1974-11-08 | ||
| JPS5265075A (en) | 1975-11-27 | 1977-05-30 | Nippon Peroxide Co Ltd | Grwoth stimulater for mattgrass |
| US4554171A (en) * | 1983-05-23 | 1985-11-19 | Bowling Clarence C | Process for coating rice seed with calcium peroxide by chemical reaction on the seed |
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2010
- 2010-12-14 CN CN2010105869000A patent/CN102119595B/en not_active Expired - Fee Related
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JPS49117244A (en) | 1973-03-23 | 1974-11-08 | ||
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| CN1869084A (en) * | 2006-04-30 | 2006-11-29 | 王树禹 | Superstrong water absorber and its preparation method and selenium-enriched plant antidraught agent using same as raw material |
| CN1994090A (en) * | 2006-12-19 | 2007-07-11 | 王树禹 | A cold- and draught- resistant seed coating agent and a cold-resistant sprinkling agent |
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Non-Patent Citations (1)
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| CN102119595A (en) | 2011-07-13 |
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Assignee: Hunan Haobo Organic Agriculture Co. Ltd. Assignor: Hunan Agricultural University Contract record no.: 2012430000124 Denomination of invention: Method for improving germination ratio of triploid seedless watermelon seeds Granted publication date: 20120215 License type: Exclusive License Open date: 20110713 Record date: 20120607 |
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