CN103340150B - A kind of method of ethylmethane sulfonate mutation process jatropha curcas seed - Google Patents
A kind of method of ethylmethane sulfonate mutation process jatropha curcas seed Download PDFInfo
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- CN103340150B CN103340150B CN201310307293.3A CN201310307293A CN103340150B CN 103340150 B CN103340150 B CN 103340150B CN 201310307293 A CN201310307293 A CN 201310307293A CN 103340150 B CN103340150 B CN 103340150B
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Abstract
The invention discloses a kind of method of ethylmethane sulfonate mutation process jatropha curcas seed.Of the present invention is after first carrying out 30 DEG C of pre-vernalization process to jatropha curcas seed, selects broken shell and to show money or valuables one carries unintentionally and the seed that radicle does not stretch out is soaked in the EMS solution of 0.9%, soak 7 hours after 3-7 days; Seed after process carries out vernalization in 28 DEG C again, incubation time 3-4 days; The planting seed survived after mutagenic treatment is in soft peat soil matrix, and down, earthing 1cm, carries out nursery to radicle.The method overcome the deficiencies in the prior art, decrease mutagenesis concentration and the mutation time of ethylmethane sulfonate, improve the efficiency of inducing mutation of ethylmethane sulfonate to jatropha curcas seed, be conducive to improving Jatropha curcas mutation breeding, promote the industrialized development of Jatropha curcas.
Description
Technical field
The present invention relates to Phytochemistry mutation breeding technologies field, particularly the method for a kind of ethylmethane sulfonate (EMS) mutagenic treatment jatropha curcas seed.
Background technology
Energy-source plant Jatropha curcas (JatrophacurcasL.), also known as Jatropha curcus, cream paulownia, little tung oil tree etc., is Euphorbiaceae Jatropha defoliation small arbor or shrub, drought-enduring resistance to thin and weak, is mainly distributed in subtropical and tropical zones.Jatropha curcas is one of plant that seed oil content is higher, is the desirable feedstock of internationally recognized preparation biodiesel, and has higher comprehensive utilization value.Jatropha curcas is listed in biodiesel gives priority to object because it has the advantage of " do not strive with grain ground, do not strive grain with people ".Although curcas biological diesel oil industry has presented fast-developing situation, realize curcas biological diesel oil industrialized development has also needed the longer cycle.This is mainly due to as a new industry, and Jatropha curcas development is still faced with numerous uncertainties, and wherein topmost uncertainty shows as the yield potential of Jatropha curcas.Jatropha curcas L due to various places plantation is all the wild or seminatural germ plasm resource from unmodified mostly, the Jatropha curcas improved seeds of high yield are lacked on large area is produced, cause its seed production low and unstable, the demand of Biodiesel extensive development cannot be met.
Owing to adopting Jatropha curcas hereditary basis comparatively narrow, being difficult to obtain by Screening germplasm and conventional breeding can the high yielding variety of spread.Therefore, utilize mutagenesis method to be conducive to improving the mutation rate of Jatropha curcas, enrich the hereditary basis of Jatropha curcas, for the acquisition of high yield Jatropha curcas mutant from now on and the seed selection of Jatropha curcas high yielding variety (strain) lay the foundation.Chinese Patent Application No. CN201010500008.6 discloses " a kind of method of Jatropha curcas M_2 seed of High-efficient Production EMS process ", it is characterized in that pre-treatment step, collects jatropha curcas seed, and carries out pre-treatment to seed; Mutagenic treatment step, the EMS solution of debita spissitudo is utilized to carry out mutagenesis process to jatropha curcas seed suitably long-time, in mutagenesis processing procedure, also stir, to ensure that the even and all jatropha curcas seed of EMS solution concentration can fully contact with EMS solution simultaneously; Nursery, transplant step, carry out nursery and transplant, to cultivate Jatropha curcas M_1 plant in good time by the Jatropha curcas M_1 seed through mutagenic treatment; Pollination self step, after the Jatropha curcas M_1 plant cultivated enters flowering stage, carries out pollination self to it, namely obtains Jatropha curcas M_2 seed after fruit maturation.
Jatropha curcas is oil plants, and seed oil content is higher, affects the many factors of seed germination rate, and the optimization process time that seed proposes after soaking after EMS mutagenic treatment and concentration lack accuracy.But removal kind of shell and seed coat carry out EMS process again, cause the workload of process in early stage excessive.Therefore correlation technique immature, also has space perfect further in actual production.
Summary of the invention
For the deficiency that prior art exists, the object of the present invention is to provide the method for a kind of ethylmethane sulfonate (EMS) mutagenic treatment jatropha curcas seed, the method is conducive to obtaining improved seeds, improves output.
Object of the present invention is achieved through the following technical solutions:
The method of a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention, comprises the following steps:
(1) the pre-vernalization of seed: choose full jatropha curcas seed, clear water soaks;
(2) seeds mutagenesis process: the seed that selecting step (1) obtains, is soaked in ethylmethane sulfonate solution;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soaks, obtain germination seed;
(4) nursery: germination seed kind step (3) obtained, in planting tray, carries out nursery;
Seed in described step (2) is show money or valuables one carries unintentionally and the seed that do not stretch out of radicle through the broken shell of preliminary vernalization.
Preferably, in step (1), described full jatropha curcas seed is the full jatropha curcas seed of this season, and described clear water soaks for seed is placed in distilled water, and under room temperature, immersion is after 24 hours, picks up at draining latter 30 DEG C and cultivates vernalization 3-7 days.
Preferably, between the culture period of above-mentioned 3-7 days, drain after seed distilled water being got express developed every 2 days, then cultivate vernalization at carrying out 30 DEG C.
Preferably, in step (2), described ethylmethane sulfonate solution be with volume mass percent concentration be 0.1%, pH value be 7.0 phosphate buffer preparation, the volume mass percent concentration of ethylmethane sulfonate is 0.9%.
Preferably, in described step (2), the time of described immersion is 7 hours.
Preferably, in described step (3), seed is put into clear water and soak for cleaning 3-4 time with distilled water, then be soaked in distilled water 10 minutes, pull out and drain, within 3-4 days, carry out vernalization in 28 DEG C of cultivations.
Preferably, in described step (4), by germination seed kind in planting tray for will the planting seed that survive after mutagenic treatment in soft peat soil matrix, down, earthing 1cm, waters radicle, then earthing is to exposing without seed.
Compared with prior art, advantage of the present invention is: the present invention selects the broken shell through preliminary vernalization to show money or valuables one carries unintentionally and the seed that do not stretch out of radicle, avoid the impact that the variation of oilseed self germination rate is larger, determine the conditional parameters such as the concentration of Best Times, temperature, solution in ethylmethane sulfonate mutation process, decrease time and the concentration of ethylmethane sulfonate mutation jatropha curcas seed, improve the efficiency of inducing mutation of ethylmethane sulfonate, be conducive to improving Jatropha curcas mutation breeding, promote the industrialized development of Jatropha curcas.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail, but embodiments of the present invention are not limited thereto.
The improvement method of EMS mutagenic treatment jatropha curcas seed of the present invention carries out in south China, Tianhe District, Guangzhou City, Guangdong Province innovation center.
First preferred embodiment of the method for embodiment 1 a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention
The present embodiment is a preferred embodiment of the method for a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention.The present embodiment adopts the improvement method of ethylmethane sulfonate mutation jatropha curcas seed, comprises following concrete steps:
(1) the pre-vernalization of seed: choose the jatropha curcas seed that this season is full, seed is placed in distilled water, soaks after 24 hours under room temperature, picks up at draining latter 30 DEG C and cultivate vernalization 7 days, in another embodiment, cultivates vernalization 5 days; Period, drain after seed distilled water being got express developed every 2 days, then cultivate vernalization at carrying out 30 DEG C;
(2) seeds mutagenesis process: the broken shell through preliminary vernalization that selecting step (1) obtains shows money or valuables one carries unintentionally and the seed that do not stretch out of radicle, is soaked in ethylmethane sulfonate solution; Described ethylmethane sulfonate solution be with phosphate buffer preparation volume mass percent concentration be 0.9% ethylmethane sulfonate solution; The volume mass percentage of phosphate buffer is 0.1%, and pH value is 7.0, and the time of immersion is 7 hours;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soak for clean 3 times with distilled water, then be soaked in distilled water 10 minutes, pull out and drain, carry out vernalization in 3 days in 28 DEG C of cultivations, obtain germination seed;
(4) nursery: planting seed step (3) survived after mutagenic treatment is in soft peat soil matrix, and down, earthing 1cm, waters radicle, then earthing is to exposing without seed.
Second preferred embodiment of the method for embodiment 2 a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention
Second preferred embodiment of the method for a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention, comprises the following steps:
(1) the pre-vernalization of seed: choose full jatropha curcas seed, clear water soaks;
(2) seeds mutagenesis process: show money or valuables one carries unintentionally and the seed that do not stretch out of radicle through the broken shell of preliminary vernalization in selecting step (1), be soaked in ethylmethane sulfonate solution;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soaks, obtain germination seed;
(4) nursery: germination seed kind step (3) obtained, in planting tray, carries out nursery.
3rd preferred embodiment of the method for embodiment 3 a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention
A preferred embodiment of the method for a kind of ethylmethane sulfonate mutation process jatropha curcas seed of the present invention, comprises the following steps:
(1) the pre-vernalization of seed: choose the jatropha curcas seed that this season is full, seed is placed in distilled water, soaks after 24 hours under room temperature, picks up at draining latter 30 DEG C and cultivate vernalization 7 days; Between the culture period of above-mentioned 7 days, drain after seed distilled water being got express developed every 2 days, then cultivate vernalization at carrying out 30 DEG C.
(2) seeds mutagenesis process: the broken shell through preliminary vernalization that selecting step (1) obtains shows money or valuables one carries unintentionally and the seed that do not stretch out of radicle, is soaked in ethylmethane sulfonate solution; Described ethylmethane sulfonate solution be with volume mass percent concentration be 0.1%, pH value be 7.0 phosphate buffer preparation, the volume mass percent concentration of described ethylmethane sulfonate is 0.9%, and the time of immersion is 7 hours;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soak for clean 4 times with distilled water, then be soaked in distilled water 10 minutes, pull out and drain, carry out vernalization in 4 days in 28 DEG C of cultivations, obtain germination seed;
(4) nursery: planting seed step (3) survived after mutagenic treatment is in soft peat soil matrix, and down, earthing 1cm, waters radicle, then earthing is to exposing without seed.
A preferred embodiment of embodiment 4 routine mutagenesis processing method
Routine mutagenesis processing method is carried out as follows:
(1) the pre-vernalization of seed: choose full jatropha curcas seed, clear water soaks;
(2) seeds mutagenesis process: by the seed in step (1), is soaked in ethylmethane sulfonate solution;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soaks, obtain germination seed;
(4) nursery: germination seed kind step (3) obtained, in planting tray, carries out nursery.
The comparative experiments of embodiment 5 ethylmethane sulfonate different disposal time
The processing time that ethylmethane sulfonate is different is set, compares percentage of seedgermination.Concrete operation step is as follows:
(1) the pre-vernalization of seed: choose full jatropha curcas seed, clear water soaks;
(2) seeds mutagenesis process: show money or valuables one carries unintentionally and the seed that do not stretch out of radicle through the broken shell of preliminary vernalization in selecting step (1), be soaked in the ethylmethane sulfonate solution of 1.0%, soak time is set to 2,4,6,8,10,12 hours;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soak for cleaning 3-4 time with distilled water, then be soaked in distilled water 10 minutes, pull out and drain, carry out vernalization for 4 days in 28 DEG C of cultivations, obtain germination seed;
(4) half lethal time is determined: investigation seed number, lethal seed number and percentage of seedgermination, utilize statistical software determination half lethal time.
Table 1 is the percentage of seedgermination of ethylmethane sulfonate different disposal time.Table 2 is the medial lethal dose analysis of ethylmethane sulfonate different disposal time.The result of the test of table 2 shows, soak time estimated value is can reach seed semilethal after 6.988 hours, therefore determines that the soak time of ethylmethane sulfonate is 7 hours.
Table 1
| Time (h) | Germination rate (%) |
| 2 | 82.76 |
| 4 | 83.33 |
| 6 | 66.67 |
| 8 | 41.67 |
| 10 | 25.00 |
| 12 | 3.13 |
Table 2
The comparative experiments of the ethylmethane sulfonate process of embodiment 6 variable concentrations
The same batch of seeds of ethylmethane sulfonate process of variable concentrations, compares the germination rate of seed.Concrete operation step is as follows:
(1) the pre-vernalization of seed: choose full jatropha curcas seed, clear water soaks;
(2) seeds mutagenesis process: show money or valuables one carries unintentionally and the seed that do not stretch out of radicle through the broken shell of preliminary vernalization in selecting step (1), be soaked in ethylmethane sulfonate solution, described ethylmethane sulfonate solution is the ethylmethane sulfonate solution of the phosphate buffer containing certain volume mass percent concentration, in the present embodiment, this concentration is set to 0,0.5,1.0,1.2,1.5,2.0%, and soak time is 8 hours;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soak for cleaning 3-4 time with distilled water, then be soaked in distilled water 10 minutes, pull out and drain, carry out vernalization for 4 days in 28 DEG C of cultivations, obtain germination seed;
(4) half lethal concentration is determined: investigation seed number, lethal seed number and percentage of seedgermination, utilize statistical software determination half lethal concentration.
Table 3 is the percentage of seedgermination of the ethylmethane sulfonate process seed of variable concentrations.Table 4 is the medial lethal dose analysis of variable concentrations ethylmethane sulfonate.Experimental result shows, concentration for the treatment of estimated value is can reach seed semilethal after 0.9%, therefore determines that the concentration for the treatment of of ethylmethane sulfonate is 0.9%.
Table 3
| Concentration (%) | Germination rate (%) |
| 0.0 | 100.00 |
| 0.5 | 66.67 |
| 1.0 | 46.67 |
| 1.2 | 41.67 |
| 1.5 | 6.67 |
| 2.0 | 0.00 |
Table 4
The comparative experiments of plant aberration rate is survived after the seed seedling-raising of embodiment 7 embodiment 1,2,3,4
Survive the aberration rate of plant after the seed seedling-raising of comparing embodiment 1,2,3,4, comprise multiple proterties such as plant height, leaf, male and female flowering, fecundity and carry out investigation variation.
Table 5 is aberration rate comparative result.Result of the test shows that the aberration rate surviving plant of the seed that method of the present invention obtains than conventional method is more stable, and higher than the aberration rate of conventional method.
Table 5
| Embodiment | Aberration rate (%) |
| Embodiment 1 | 19.64％ |
| Embodiment 2 | 18.92％ |
| Embodiment 3 | 19.78％ |
| Embodiment 4 | 15％ |
Claims (3)
1. a method for ethylmethane sulfonate mutation process jatropha curcas seed, comprises the following steps:
(1) the pre-vernalization of seed: choose full jatropha curcas seed, clear water soaks; Described full jatropha curcas seed is the full jatropha curcas seed of this season, described clear water soaks for seed is placed in distilled water, soak under room temperature after 24 hours, pick up at draining latter 30 DEG C and cultivate vernalization 3-7 days, during described cultivation 3-7 days, drain after seed distilled water being got express developed every 2 days, then cultivate vernalization at carrying out 30 DEG C;
(2) seeds mutagenesis process: the seed that selecting step (1) obtains, is soaked in ethylmethane sulfonate solution; Described ethylmethane sulfonate solution be with quality concentration of volume percent be 0.1%, pH value be 7.0 phosphate buffer preparation, the quality concentration of volume percent of described ethylmethane sulfonate is 0.9%;
(3) mutagenesis presprouting of seeds: the seed that step (2) obtains is put into clear water and soaks, obtain germination seed, specifically seed being put into clear water soaks for cleaning 3-4 time with distilled water, be soaked in distilled water again 10 minutes, pull out and drain, within 3-4 days, carry out vernalization in 28 DEG C of cultivations;
(4) nursery: germination seed kind step (3) obtained, in planting tray, carries out nursery;
It is characterized in that,
Seed in described step (2) is show money or valuables one carries unintentionally and the seed that do not stretch out of radicle through the broken shell of preliminary vernalization.
2. the method for ethylmethane sulfonate mutation process jatropha curcas seed according to claim 1, is characterized in that, in described step (2), the time of described immersion is 7 hours.
3. the method for ethylmethane sulfonate mutation process jatropha curcas seed according to claim 1, it is characterized in that, in described step (4), by germination seed kind in planting tray for will the planting seed that survive after mutagenic treatment in soft peat soil matrix, radicle down, earthing 1cm, waters, then earthing is to exposing without seed.
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| CN104542265A (en) * | 2013-10-25 | 2015-04-29 | 北京林业大学 | Mutation breeding method for cunninghamia lanceolata |
| CN105010131A (en) * | 2015-07-27 | 2015-11-04 | 杭州市农业科学研究院 | Operation method for salvia splendens EMS(Ethyl methane sulfonate) mutation breeding |
| CN105210862B (en) * | 2015-09-18 | 2017-09-15 | 云南省烟草农业科学研究院 | A kind of vegetable seeds method of mutagenesis and device |
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| CN105875404B (en) * | 2016-04-13 | 2018-05-29 | 广东省农业科学院作物研究所 | A kind of method of ethylmethane sulfonate mutation processing sugarcane |
| CN106068757B (en) * | 2016-06-14 | 2018-10-09 | 泰安市泰山林业科学研究院 | A kind of method of mutagenesis of wingceltis coloured silk leaf kind |
| CN111685036B (en) * | 2020-06-07 | 2021-07-23 | 广东省农业科学院作物研究所 | Method for mutagenizing andrographis paniculata by ethyl methanesulfonate |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101801176A (en) * | 2007-09-24 | 2010-08-11 | 凯津公司 | Method for the selection of plants with specific mutations |
| CN102017891A (en) * | 2010-10-08 | 2011-04-20 | 普罗米绿色能源(深圳)有限公司 | Method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester) |
| CN102907317A (en) * | 2012-11-06 | 2013-02-06 | 中华全国供销合作总社南京野生植物综合利用研究所 | Method for inducing euphorbia lathyris seeds and screening salt-tolerant mutants |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| IL121750A0 (en) * | 1997-09-11 | 1998-02-22 | Yeda Res & Dev | Method for large scale mutagenesis and gene knockout in crop plants |
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Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101801176A (en) * | 2007-09-24 | 2010-08-11 | 凯津公司 | Method for the selection of plants with specific mutations |
| CN102017891A (en) * | 2010-10-08 | 2011-04-20 | 普罗米绿色能源(深圳)有限公司 | Method for efficiently producing jatropha M_2 seeds processed by EMS (methanesulfonic acid ethyl ester) |
| CN102907317A (en) * | 2012-11-06 | 2013-02-06 | 中华全国供销合作总社南京野生植物综合利用研究所 | Method for inducing euphorbia lathyris seeds and screening salt-tolerant mutants |
Non-Patent Citations (1)
| Title |
|---|
| 甲基磺酸乙酯( EMS)处理对麻疯树( Jatropha curcas L.)萌发种子的生理效应;徐刚;《种子》;20101025;第29卷(第10期);第49页摘要，第50页左栏"材料处理和种子萌发"部分 * |
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