CN105532406A - White-pulp loquat new strain preparing method based on red-pulp loquat variety - Google Patents
White-pulp loquat new strain preparing method based on red-pulp loquat variety Download PDFInfo
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- CN105532406A CN105532406A CN201610021365.1A CN201610021365A CN105532406A CN 105532406 A CN105532406 A CN 105532406A CN 201610021365 A CN201610021365 A CN 201610021365A CN 105532406 A CN105532406 A CN 105532406A
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- loquat
- seedling
- seedlet
- white
- white pulp
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G31/00—Soilless cultivation, e.g. hydroponics
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
Abstract
The invention discloses a white-pulp loquat new strain preparing method based on a red-pulp loquat variety. The method comprises the steps of taking seedlets out of mature fruits of the red-pulp loquat variety, and seeding the seedlets on a cultivation medium for seedling cultivation, wherein the cultivation medium is prepared from 100-200 mg/L of EMS, 2-5 mg/L of GA, 0.5 mg/L of 6-BA, 0.1 mg/L of NAA, 0.1 mg/L of IBA, activated carbon and an agar MS cultivation medium; conducting seedling hardening and transplantation to obtain a white-pulp loquat new strain after blossoming and fruiting. By the adoption of the method, the limitation that only a white-pulp loquat variety can be used as the initial material for breeding is broken, cost is low, no special instrument or reagent is needed, the application range is wide, and the success rate is high. The requirement of seedlets on cultivation conditions is low, seedling cultivation is easy, seedlings are robust, the transplantation survival rate of cultivated seedlings can be over 90%, and the white-pulp loquat plant obtaining rate can be about 4%.
Description
Technical field
The invention belongs to field of plant growing technology, particularly relate to a kind of White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars.
Background technology
Loquat is the peculiar subtropical fruit tree originating in China, and fruit is on the occasion of fruit listing in dull season at the end of spring and the beginning of summer, and nutritious, local flavor is splendid, is loved by the people.Loquat Cultivars can be divided into plain boiled pork (white sand) Loquat Cultivars and red meat (red sand) Loquat Cultivars by pulp colour.The pulp milky or faint yellow of White Pulp Loquat kind, fine and tender taste, juice multi-flavor is sweet, and local flavor is splendid, and its endoplasm is better than red meat kind, is most appropriate to eat raw.Although the fruit quality of White Pulp Loquat kind (endoplasm) is better than red meat kind, but the principal item of whole world loquat cultivation is at present red meat kind, plain boiled pork kind mainly occupies certain proportion on the Jiangsu in China's loquat the northern fringe producing region, Zhejiang, Shanghai and other places, and on other market, loquat producing region, difficulty is shown in its trace.Abroad, also there are a small amount of plain boiled pork kind in Spain and Japan, but form commercial scale production.Cause this situation major reason to be the negligible amounts of plain boiled pork kind, applicable improved seeds can not be found in each producing region from limited plain boiled pork variety source.Therefore, large scale cultivating White Pulp Loquat improved seeds are task of top priority of adjustment China and even global loquat industry breed structure.And want large scale cultivating White Pulp Loquat improved seeds, breeding method innovation is then crucial.Up to now, White Pulp Loquat kind is mainly from three approach: one is directly selected by seed selection of growing directly from seeds from plain boiled pork germ plasm resource, and most of plain boiled pork cultivar origin is in this approach; Two by with plain boiled pork kind for parent is cultivated by crossbreeding approach, minority plain boiled pork cultivar origin is in this approach; Three find from bud mutation, and indivedual plain boiled pork cultivar origin is in this approach.
On the whole, existing White Pulp Loquat breeding for quality approach is less, and efficiency is lower, therefore, cultivates the White Pulp Loquat kind can applied on producing in a large number and still shoulders heavy responsibilities, and the method for research and development efficient initiative loquat plain boiled pork kind is still significant.
Summary of the invention
The object of the present invention is to provide a kind of White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars, be intended to solve White Pulp Loquat breeding for quality approach in prior art less, the problem that efficiency is lower.
The present invention realizes like this, a kind of White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars, the described White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars comprises: when red meat Loquat Cultivars fruit proceeds to maturation, from fruit, take out seedlet, be inoculated on medium and cultivate seedling;
Described medium is the MS medium containing EMS100 ~ 200mg/L, GA2 ~ 5mg/L, 6-BA0.5mg/L, NAA0.1mg/L, IBA0.1mg/L, active carbon 0.1% and agar 0.6%.
Further, the condition of culture of described cultivation seedling is temperature 25 ± 1 DEG C, cultivates 15 days, then under the condition of illumination 2000 ± 500lux, 18h/d, cultivate one month under dark condition.
Further, described seedling, after growing up to complete seedling strain, carries out hardening, and method is:
Blake bottle is moved on to lower 2 days of 15 DEG C ~ 25 DEG C conditions, allow the seedlet seedling cultivated adapt to the change of extraneous light and temperature gradually;
Then throw off the aseptic sealed membrane of blake bottle, make outside air enter in blake bottle, seedlet seedling obtained the resistance of microorganism under normal natural conditions and adapts to the change of outside air humidity, after 5 days, loquat seedling being taken out in blake bottle;
Clean the medium on loquat shoot root, transplant in the perlite of sterilizing and the basin of peat 1:1 mixed-matrix, cultivate under being placed in enclosed environment condition, incubation maintenance natural lighting, nearly 100% relative moisture, 25 ± 1 DEG C.Within every two weeks, apply fertilizer once with MS medium nutrient solution;
Remove SLD gradually after 1 month, reduce the relative moisture of loquat seedling growing environment gradually to natural level, move in ordinary nutritional soil when seedling starts to grow young leaves, nursery stock manages routinely.
Further, by described seedlet plantlet of transplant to field, culture technique management routinely, plant to be planted identifies White Pulp Loquat new lines after yielding positive results.
White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars provided by the invention, with red meat Loquat Cultivars for material can formulate out White Pulp Loquat new lines, without the need to being confined to White Pulp Loquat kind as breeding starting material, simple to operate, low cost, do not need special instrument and equipment and reagent, and widely applicable, success rate is high, be applicable to apply.Loquat seedlet of the present invention requires low to condition of culture, and easily cultivate seedling, the growth of seedling is also healthy and strong, and the transplanting survival rate cultivating the seedlet seedling strain obtained can reach more than 90%.The method that the present invention prepares White Pulp Loquat plant can cultivate White Pulp Loquat plant from red meat loquat, and frequency can up to about 4%.The present invention prepares the method for White Pulp Loquat plant, and require low to instrument and equipment, step is easy, low cost, has good actual application prospect.
Accompanying drawing explanation
Fig. 1 is the White Pulp Loquat new lines preparation method flow chart based on red meat Loquat Cultivars that the embodiment of the present invention provides.
Fig. 2 is the seedlet design sketch that the embodiment of the present invention provides.
Embodiment
In order to make object of the present invention, technical scheme and advantage clearly understand, below in conjunction with embodiment, the present invention is further elaborated.Should be appreciated that specific embodiment described herein only in order to explain the present invention, be not intended to limit the present invention.
Below in conjunction with accompanying drawing, the present invention is explained in detail.
As shown in Figure 1, the White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars of the embodiment of the present invention comprises the following steps:
S101: when fruit is about to proceed to maturation, take out seedlet from fruit;
S102: seedlet is inoculated on medium and cultivates seedling;
S103: hardening is carried out to seedlet test-tube plantlet;
S104: cultivate in seedlet transplantation of seedlings to nutritive cube;
S105: seedlet Potted orchard is transplanted to field and cultivates;
S106: identify White Pulp Loquat new lines after plant blossom result.
In an embodiment of the present invention:
In described step S102: the in-vitro culture method of seedlet comprises: be inoculated in by seedlet on medium, cultivate seedling.
Described medium is: the MS medium containing EMS100 ~ 200mg/L, GA2 ~ 5mg/L, 6-BA0.5mg/L, NAA0.1mg/L, IBA0.1mg/L, active carbon 0.1% and agar 0.6%.
Condition of culture is temperature 25 ± 1 DEG C, cultivates 15 days, then under the condition of illumination 2000 ± 500lux, 18h/d, cultivate one month under dark condition.
In described step S103: the method for seedlet test-tube plantlet being carried out to hardening is:
Blake bottle is moved on to lower 2 days of 15 DEG C ~ 25 DEG C conditions, allow the seedlet seedling cultivated adapt to the change of extraneous light and temperature gradually;
Then the aseptic sealed membrane of blake bottle is thrown off, make outside air enter in blake bottle, seedlet seedling is obtained the resistance of microorganism under normal natural conditions and adapts to the change of outside air humidity, after 5 days, seedlet seedling is taken out in blake bottle, is transplanted in nutritive cube.
In described step S104: by the method for cultivating in seedlet transplantation of seedlings to nutritive cube be:
Clean the medium on seedlet shoot root, transplant in the perlite of sterilizing and the basin of peat 1:1 mixed-matrix, cultivate under being placed in enclosed environment condition, incubation maintenance natural lighting, nearly 100% relative moisture, 25 ± 1 DEG C.Within every two weeks, apply fertilizer once with MS medium nutrient solution;
Remove SLD gradually after 1 month, reduce the relative moisture of loquat seedling growing environment gradually to natural level, move in ordinary nutritional soil when seedling starts to grow young leaves, nursery stock manages routinely.
In described step S105: seedlet Potted orchard is transplanted to field and cultivates, method is that culture technique manages routinely.
In described step S106: the method identifying White Pulp Loquat new lines after plant blossom result identifies according to pulp colour, the pulp colour of White Pulp Loquat new lines is white, very clear.
Below in conjunction with specific embodiment, the invention will be further described.
The preparation of embodiment 1 seedlet
1, well-grown loquat plant (kind is ' large five-pointed star ' or ' No. 1, dragon's fountain ') is chosen, when fruit development is about 1.5cm to diameter (Ya'an Sichuan province is about the first ten-day period of the March), dredge and go to grow abnormal fruit, each fruit ear is made to stay 3 ~ 4 fruits, so that fruit can obtain sufficient nutrition and reach full growth.
2, the first tenday period of a month in the last ten-days period in April to May (Ya'an Sichuan province), when loquat diameter reach about 3cm, surperficial fine hair is sloughed off, when fruit is about to proceed to maturation (pericarp starts to turn yellow, pulp start deliquescing), by under Fruit, in flowing water, fruit is cleaned.Under aseptic condition on superclean bench, fruit is cut, take out seedlet (Fig. 1) (seedlet diameter is about 0.1-0.2cm, and common seed diameter is more than 1cm).
The preparation of embodiment 2 seedlet plant
By the seedlet of preparation in embodiment 1, be inoculated in add EMS100 ~ 200mg/L, GA2 ~ 5mg/L, 6-BA0.5mg/L, NAA0.1mg/L, IBA0.1mg/L, active carbon 0.1% and agar 0.6% MS medium on, cultivate 15 days under being placed in 25 ± 1 DEG C of dark conditions, then temperature 25 ± 1 DEG C, illumination 18h, night, 6h, cultivated seedling (about cultivating month) under 1500lux light intensity.
After growing up to complete seedling strain, carry out hardening, to adapt to external environment.Hardening off method is: blake bottle is moved on to lower 2 days of room temperature (15 ~ 25 DEG C) condition, allows the seedlet seedling cultivated adapt to the change of extraneous light and temperature gradually; Then throw off the aseptic sealed membrane of blake bottle, make outside air enter in blake bottle, seedlet seedling is obtained the resistance of microorganism under normal natural conditions and adapts to the change of outside air humidity.After 5 days, loquat seedling is taken out in blake bottle, cleans the medium on seedlet shoot root, transplant in the perlite of sterilizing and the basin of peat 1:1 mixed-matrix, cultivate under being placed in enclosed environment condition, incubation keep natural lighting, nearly 100% relative moisture, 25 ± 1 DEG C.Within every two weeks, apply fertilizer once with MS nutrient solution (i.e. MS liquid nutrient medium).Remove SLD gradually after 1 month, the relative moisture reducing loquat seedling growing environment gradually, to natural level, finally removes SLD.Move in ordinary nutritional soil when seedling starts to grow young leaves, nursery stock manages routinely.
The qualification of embodiment 3 White Pulp Loquat plant
Be transplanted to orchard after seedlet nutritious bag seedling cultivates 1 year, adopt conventional cultivation technology to manage.After plant to be planted is yielded positive results (about 5-6), according to pulp colour qualification and statistics White Pulp Loquat plant, with formula: White Pulp Loquat plant pick-up rate=acquisitions White Pulp Loquat plant number/acquisition plant total * 100% calculates White Pulp Loquat plant pick-up rate (the results are shown in Table 1).
The preparation result of table 1 White Pulp Loquat plant
In the embodiment above, MS medium (MurashigeandSkoogmedium)
Formula:
Macroelement: CALCIUM CHLORIDE DIHYDRATE (CaCl
2) 440.0mg/L, potassium dihydrogen phosphate (KH
2pO
4) 170.0mg/L, potassium nitrate (KNO
3) 1900.0mg/L, bitter salt (MgSO
47H
2o) 370.0mg/L, ammonium nitrate (NH
4nO
3) 1650.0mg/L; Trace element: Salzburg vitriol (CuSO
45H
2o) 0.025mg/L, molysite (FeNaEDTA) 36.7mg/L, boric acid (H
3bO
3) 6.2mg/L, Manganous sulfate monohydrate (MnSO
4h
2o) 16.9mg/L, Zinc vitriol (ZnSO
47H
2o) 8.6mg/L, four molybdic acid hydrate sodium (Na
2mo
44H
2o) 0.25mg/L, potassium iodide (KI) 0.83mg/L.Organic matter: glycine (Glycine) 2.0mg/L, inositol (Myo-inositol) 100mg/L, nicotinic acid (Nicotinicacid) 0.5mg/L, VB60.5mg/L, VB10.1mg/L.
In addition, the full name of filling a prescription of more than abridging is respectively, EMS: ethylmethane sulfonate (ethylmethanesulfonate), GA: gibberellic acid (geberellicacid), IBA: indolebutyric acid (indolebutyricacid), NAA: methyl α-naphthyl acetate (α-naphthaleneaceticacid), 6-BA:6-benzyl purine (6-benzyladenine).
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any amendments done within the spirit and principles in the present invention, equivalent replacement and improvement etc., all should be included within protection scope of the present invention.
Claims (5)
1. the White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars, it is characterized in that, the described White Pulp Loquat new lines preparation method based on red meat Loquat Cultivars comprises: when red meat Loquat Cultivars fruit proceeds to maturation, from fruit, take out seedlet, be inoculated on medium and cultivate seedling;
Described medium contains the MS medium of EMS100 ~ 200mg/L, GA2 ~ 5mg/L, 6-BA0.5mg/L, NAA0.1mg/L, IBA0.1mg/L, active carbon 0.1% and agar 0.6%.
2. as claimed in claim 1 based on the White Pulp Loquat new lines preparation method of red meat Loquat Cultivars, it is characterized in that, the condition of culture that described seedlet cultivates seedling is temperature 25 ± 1 DEG C, cultivate 15 days under dark condition, then, under the condition of illumination 2000 ± 500lux, 18h/d, cultivate one month.
3., as claimed in claim 1 based on the White Pulp Loquat new lines preparation method of red meat Loquat Cultivars, it is characterized in that, described seedling, after growing up to complete seedling strain, carries out hardening, and method is:
Blake bottle is moved on to lower 2 days of 15 DEG C ~ 25 DEG C conditions, allow the seedlet seedling cultivated adapt to the change of extraneous light and temperature gradually;
Then throw off the aseptic sealed membrane of blake bottle, make outside air enter in blake bottle, seedlet seedling obtained the resistance of microorganism under normal natural conditions and adapts to the change of outside air humidity, after 5 days, loquat seedling being taken out in blake bottle;
Clean the medium on loquat shoot root, transplant in the perlite of sterilizing and the basin of peat 1:1 mixed-matrix, cultivate under being placed in enclosed environment condition, incubation maintenance natural lighting, nearly 100% relative moisture, 25 ± 1 DEG C.Within every two weeks, apply fertilizer once with MS medium nutrient solution;
Remove SLD gradually after 1 month, reduce the relative moisture of growing environment gradually to natural level, move in ordinary nutritional soil when seedling starts to grow young leaves, nursery stock manages routinely.
4. as claimed in claim 1 based on the White Pulp Loquat new lines preparation method of red meat Loquat Cultivars, it is characterized in that, described seedlet plant is transplanted to field, culture technique management routinely cultivate 1 year in ordinary nutritional soil basin alms bowl after.
5., as claimed in claim 1 based on the White Pulp Loquat new lines preparation method of red meat Loquat Cultivars, it is characterized in that, after being transplanted to the plant blossom result in field, identify White Pulp Loquat new lines.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN116138168A (en) * | 2023-02-17 | 2023-05-23 | 北京林业大学 | Culture medium for promoting germination of raspberry seeds and tissue culture seedling method |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN116138168A (en) * | 2023-02-17 | 2023-05-23 | 北京林业大学 | Culture medium for promoting germination of raspberry seeds and tissue culture seedling method |
| CN116138168B (en) * | 2023-02-17 | 2024-04-05 | 北京林业大学 | Culture medium for promoting germination of raspberry seeds and tissue culture seedling method |
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