CN116040191A - Sample transfer device for biological sample library and transfer method thereof - Google Patents

Sample transfer device for biological sample library and transfer method thereof Download PDF

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Publication number
CN116040191A
CN116040191A CN202310275545.2A CN202310275545A CN116040191A CN 116040191 A CN116040191 A CN 116040191A CN 202310275545 A CN202310275545 A CN 202310275545A CN 116040191 A CN116040191 A CN 116040191A
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CN
China
Prior art keywords
transfer
door
window body
access
assembly
Prior art date
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Pending
Application number
CN202310275545.2A
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Chinese (zh)
Inventor
赵军
邢书赫
林帅
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Zhiliang Zhikong Biotechnology Shandong Co ltd
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Zhiliang Zhikong Biotechnology Shandong Co ltd
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Application filed by Zhiliang Zhikong Biotechnology Shandong Co ltd filed Critical Zhiliang Zhikong Biotechnology Shandong Co ltd
Priority to CN202310275545.2A priority Critical patent/CN116040191A/en
Publication of CN116040191A publication Critical patent/CN116040191A/en
Pending legal-status Critical Current

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    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65GTRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
    • B65G1/00Storing articles, individually or in orderly arrangement, in warehouses or magazines
    • B65G1/02Storage devices
    • B65G1/04Storage devices mechanical
    • B65G1/137Storage devices mechanical with arrangements or automatic control means for selecting which articles are to be removed
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65DCONTAINERS FOR STORAGE OR TRANSPORT OF ARTICLES OR MATERIALS, e.g. BAGS, BARRELS, BOTTLES, BOXES, CANS, CARTONS, CRATES, DRUMS, JARS, TANKS, HOPPERS, FORWARDING CONTAINERS; ACCESSORIES, CLOSURES, OR FITTINGS THEREFOR; PACKAGING ELEMENTS; PACKAGES
    • B65D88/00Large containers
    • B65D88/74Large containers having means for heating, cooling, aerating or other conditioning of contents
    • BPERFORMING OPERATIONS; TRANSPORTING
    • B65CONVEYING; PACKING; STORING; HANDLING THIN OR FILAMENTARY MATERIAL
    • B65GTRANSPORT OR STORAGE DEVICES, e.g. CONVEYORS FOR LOADING OR TIPPING, SHOP CONVEYOR SYSTEMS OR PNEUMATIC TUBE CONVEYORS
    • B65G1/00Storing articles, individually or in orderly arrangement, in warehouses or magazines
    • B65G1/02Storage devices
    • B65G1/04Storage devices mechanical
    • B65G1/0485Check-in, check-out devices

Abstract

The invention discloses a sample transfer device for a biological sample library and a transfer method thereof, wherein the transfer device comprises a transfer window body, a transfer door driven by a transfer opening and closing mechanism is arranged on the transfer window body, an access door driven by an access opening and closing mechanism is arranged in the transfer window body, a moving platform and a synchronous belt transfer mechanism are arranged in the transfer window body to jointly drive a transfer tank to move between the transfer opening and the access opening, a precooling component for supplying cooling gas into the transfer window body and a temperature and humidity detection device are arranged at the upper part of the transfer window body, and a liquid nitrogen filling mechanism is arranged at the upper part of the transfer window body.

Description

Sample transfer device for biological sample library and transfer method thereof
Technical Field
The invention relates to the technical field of biological sample access, in particular to a sample transfer device for a biological sample library and a transfer method thereof.
Background
The biological sample library is a large-scale device for storing important biological samples at low temperature for a long time, the biological sample library is kept in a constant temperature and humidity environment for a long time, the influence of temperature and humidity in the environment outside the biological sample library on the samples in the sample library needs to be reduced as much as possible, the samples are usually placed in a special container such as a transfer tank, and then the operations of storing and transferring the samples out of the sample library are carried out through a transfer device.
The existing transfer device is usually operated manually, doors and windows of the transfer device are opened and closed manually, sealing of the doors and windows is easy to be poor, the transfer distance of the existing automatic transfer device is short, in the transfer process, samples in the transfer tank can undergo multiple changes of environmental temperature and humidity, the changes of the environmental temperature and humidity reduce the sample quality in the transfer tank, meanwhile, the environment in a sample warehouse is affected, and the refrigeration cost is increased.
Disclosure of Invention
The invention aims to overcome the defects in the prior art, provides a sample transfer device for a biological sample library and a transfer method thereof, and solves the problem that the quality of a sample is affected by repeated changes of environmental temperature and humidity in the process of sample storage and retrieval in the biological sample library.
The technical scheme adopted for solving the technical problems is as follows:
the sample transfer device for the biological sample library comprises a transfer window body and a controller, wherein the transfer window body is internally and slidably connected with a mobile platform, a driving assembly is arranged on the lower side of the mobile platform, a synchronous belt transfer mechanism for driving a transfer tank to translate is arranged on the mobile platform, a transfer port is arranged at the front part of the transfer window body and is hinged with a transfer door, the transfer door is connected with a transfer opening and closing mechanism, the mobile platform penetrates through the transfer port and is slidably connected onto a bottom plate of the transfer window body along the front-back direction, a liquid nitrogen filling mechanism and an access port are arranged at the upper part of the transfer window body, the access port is hinged with an access door, and the access door is connected with the access opening and closing mechanism;
the transfer window is internally provided with a precooling component for supplying cooling gas, a temperature and humidity detection device and a jacking component for driving the transfer tank, the jacking component corresponds to the access port from top to bottom, and the driving component, the synchronous belt conveying mechanism, the transfer opening and closing mechanism, the access opening and closing mechanism, the precooling component and the jacking component are all electrically connected with the controller.
Further, the joint position of the transfer door and the transfer port is provided with a sealing ring, and the joint position of the access door and the access port is provided with a sealing ring.
Further, the precooling assembly comprises a gas dispersing part, a connector and a control valve, one end of the connector is connected with the gas dispersing part, the other end of the connector is connected with the control valve, the control valve is communicated with an external cooling air source, the gas dispersing part comprises a shell, and a plurality of nozzles are arranged on the side face of the shell.
Further, the gas supplied by the pre-cooling assembly is cooling gas without water vapor.
Further, the jacking component comprises two sleeved guide sleeves and guide rods, the two guide sleeves are connected with the bottom plate, the upper ends of the two guide rods penetrate through the bottom plate and are connected through the jacking table, the lower ends of the two guide rods are connected through a connecting plate, the connecting plate is connected with one end of the jacking piece, the other end of the jacking piece is connected with the bottom plate, and a position sensor is arranged at the initial position of the up-and-down movement of the connecting plate.
Further, the transmission opening and closing mechanism comprises a push rod, one end of the push rod is rotationally connected with the transmission door, the other end of the push rod is rotationally connected with the screw rod nut assembly, the driving end of the screw rod nut assembly is connected with the first motor, and a position sensor is arranged at the initial position of the movement of the transmission door.
Further, the access opening and closing mechanism comprises a rotating shaft connected with the rotating end of the access door, the end part of the rotating shaft is connected with a second motor, and a position sensor is arranged at the initial position of the movement of the access door.
A sample transfer method for a biological sample library is applied to a sample transfer device for the biological sample library, and comprises the following steps:
s1, when a biological sample is stored in a biological sample library, a robot conveys a transfer tank to the side of a transfer port of a transfer window body, and a controller starts a precooling assembly to replace gas in the transfer window body;
s2, controlling the transfer opening and closing mechanism to open the transfer door, driving the front end of the mobile platform to move out of the transfer port by the driving assembly, placing the transfer tank on the synchronous belt conveying mechanism at the front end of the mobile platform by the robot, reversely moving and resetting the mobile platform, and resetting the transfer door;
s3, driving the transfer tank to move below the access port by the synchronous belt conveying mechanism;
s4, the controller starts the jacking component to drive the top of the transfer tank to be flush with the access port, and when the temperature and humidity detection device detects that the temperature and humidity in the transfer window reach the required range, the precooling component stops working;
s5, controlling an access opening and closing mechanism to open an access door, taking a cover of the transfer tank by a robot, taking samples in the transfer tank, resetting the cover, resetting the access door, and resetting the jacking component;
s6, the controller starts the precooling assembly to replace gas in the transfer window body;
s7, controlling a transfer opening and closing mechanism to open a transfer door, driving the transfer tank to move to the front end of the mobile platform by a synchronous belt conveying mechanism, driving the front end of the mobile platform to move out of a transfer port by a driving assembly, taking away the transfer tank by a robot, resetting the mobile platform, and resetting the transfer door;
and S8, when the temperature and humidity detection device detects that the temperature and humidity in the transmission window reach the required range, the precooling assembly stops working.
Further, in step S5, after the robot takes the lid of the transfer tank, the liquid nitrogen is filled into the transfer tank through the liquid nitrogen filling mechanism at the top of the transfer window, and then the sample in the transfer tank is taken, and the lid is reset.
The invention has the following beneficial effects:
1. the transfer opening and closing mechanism is arranged at the front part of the transfer window body to drive the transfer door to open and close, the access opening and closing mechanism is arranged at the upper part of the transfer window body to drive the access door to open and close, the moving platform and the synchronous belt conveying mechanism are controlled by the controller to drive the transfer tank to move between the transfer port and the access port together, the liquid nitrogen filling mechanism is arranged at the upper part of the transfer window body, the precooling component for supplying cooling gas and the temperature and humidity detection device are arranged in the transfer window body, the gas in the transfer window body is replaced by the precooling component, the environment in the transfer window body is kept at the specified temperature and humidity, the sample is filled into the transfer tank by the liquid nitrogen filling mechanism, the sample is in the specified temperature and humidity environment during waiting to be taken, the influence of repeated change of the environment temperature and humidity on the sample is effectively reduced in the sample access process in the biological sample warehouse, and the sample quality in the sample access process is ensured.
2. The pressure release valve communicated with the transfer window body is arranged at the bottom of the transfer window body, when the pressure in the transfer window body exceeds a specified value, the pressure in the transfer window body is kept within a specified range by exhausting the gas through the pressure release valve, and external environment gas is prevented from entering the transfer window body.
3. And the joint positions of the transfer door and the transfer port and the joint positions of the access door and the access port are respectively provided with a sealing ring, when the transfer door and the access door are in a closed state, the sealing rings play a role in blocking the circulation of internal and external gases, and the replacement efficiency of the precooling assembly on the gas in the transfer window body is improved.
4. The gas dispersing piece is arranged in the transmission window, the cooling gas rapidly enters the transmission window through the gas dispersing piece to carry out gas replacement, the gas replacement efficiency is improved,
5. install jacking subassembly in the transfer window, when the transfer jar is located jacking subassembly top, drive transfer jar top and access mouth parallel and level are favorable to the operation of robot access sample in to the transfer jar.
Drawings
The invention is further described with reference to the accompanying drawings:
FIG. 1 is a schematic perspective view of the present invention;
FIG. 2 is a front view of the present invention;
FIG. 3 is a right side view of the present invention;
FIG. 4 is a cross-sectional view taken at A-A of FIG. 2;
FIG. 5 is a schematic view of the structure of the present invention when the transfer door and the access door are closed;
FIG. 6 is a cross-sectional perspective view of the portion B-B of FIG. 5;
FIG. 7 is a cross-sectional view taken at B-B of FIG. 5;
FIG. 8 is an enlarged view of a portion of FIG. 1 at A;
FIG. 9 is an enlarged view of a portion of FIG. 1 at B;
FIG. 10 is an enlarged view of a portion of FIG. 6 at C;
FIG. 11 is a partial enlarged view of the portion D of FIG. 4;
in the figure, 1, a transmission window body; 11. a transfer port; 12. a transfer gate; 13. a bottom plate; 14. an access port; 15. an access door; 2. a mobile platform; 3. a drive assembly; 4. a conveying mechanism; 5. a transmission opening and closing mechanism; 51. a push rod; 53. a screw rod and nut assembly; 52. a first motor; 6. an access opening and closing mechanism; 61. a rotating shaft; 62. a second motor; 7. a pre-cooling assembly; 71. a gas dispersion member; 711. a housing; 712. a spout; 72. a joint; 8. a jacking assembly; 81. guide sleeve; 82. a guide rod; 83. a jacking table; 84. a connecting plate; 85. and a jacking piece.
Detailed Description
In order to make the technical solution of the present invention better understood by those skilled in the art, the technical solution of the present invention will be clearly and completely described below with reference to the accompanying drawings.
As shown in fig. 1-6, the invention provides a sample transfer device for a biological sample library, which comprises a transfer window body 1 and a controller, wherein a moving platform 2 and a driving component 3 for driving the moving platform 2 to move are connected in a sliding manner in the transfer window body 1, and a synchronous belt conveying mechanism 4 for driving a transfer tank to translate is arranged on the moving platform 2. The movable platform 2 is driven to move back and forth in the transmission window body 1 through the driving component 3, the transfer tank is placed on the synchronous belt conveying mechanism 4 and moves together with the movable platform 2, the transfer tank is driven to move back and forth in the transmission window body 1 through the synchronous belt conveying mechanism 4, the driving component 3 is in a gear-rack transmission mode driven by a motor, the synchronous belt conveying mechanism 4 is widely applied in the field, the structure and principle of the synchronous belt conveying mechanism are well known to those skilled in the art, and the structure and principle of the synchronous belt conveying mechanism are not described in detail.
As shown in fig. 2-4, a transmission opening 11 is arranged at the front part of the transmission window body 1, a transmission door 12 is hinged at the transmission opening 11, the transmission door 12 is connected with a transmission opening and closing mechanism 5, and the transmission opening and closing mechanism 5 drives the transmission door 12 to open towards the outer side of the transmission window body 1;
the moving platform 2 passes through the transmission port 11 and is connected to the bottom plate 13 of the transmission window body 1 in a sliding manner along the front-back direction, a position sensor is arranged at the starting position of the front-back movement of the moving platform 2, the moving platform 2 is driven by the driving component 3, the front end of the moving platform 2 extends out of the transmission port 11 or retreats into the transmission window body 1, and the transmission tank is driven to move in and out of the transmission window body 1 in a translation manner from the transmission port 11 by matching with the driving action of the synchronous belt transmission mechanism 4.
The upper part of the transfer window body 1 is provided with a liquid nitrogen filling mechanism for filling liquid nitrogen into the transfer tank and an access port 14, the access port 14 is hinged with an access door 15, the access door 15 is connected with an access opening and closing mechanism 6, and the access opening and closing mechanism 6 drives the access door 15 to open to the outer side of the transfer window body 1; the access port 14 is provided on the side of the upper portion of the transfer window 1 away from the transfer port 11, and when the transfer pot is translated below the access port 14, the driving of the timing belt transfer mechanism 4 is stopped.
The precooling assembly 7 for supplying cooling gas, the jacking assembly 8 for driving the transfer tank and the temperature and humidity detection device are arranged in the transfer window body 1, the jacking assembly 8 corresponds to the access port 14 up and down, the precooling assembly 7 is arranged close to the access port 14, the cooling of the transfer tank by the rapid cooling gas is facilitated, a pressure relief valve communicated with the inside of the transfer window body 1 is arranged at the bottom of the transfer window body 1, when the precooling assembly 7 supplies cooling gas to the transfer window body 1, and when the pressure in the transfer window body 1 exceeds a specified value, the pressure in the transfer window body 1 is kept in a specified range by exhausting the gas through the pressure relief valve, and external environment gas is prevented from entering the transfer window body 1.
When the synchronous belt conveying mechanism 4 drives the transfer tank to translate below the access port 14, the jacking component 8 is positioned at the lower side of the transfer tank; when the jacking component 8 is started to jack the transfer tank to the access port 14 and the access door 15 is opened, the liquid nitrogen filling mechanism at the upper part of the transfer window body 1 can fill liquid nitrogen into the transfer tank.
The driving component 3, the synchronous belt conveying mechanism 4, the transmission opening and closing mechanism 5, the access opening and closing mechanism 6, the precooling component 7 and the jacking component 8 are all electrically connected with the controller.
As shown in fig. 4, a sealing ring is provided at a position where the transfer door 12 is attached to the transfer port 11, and a sealing ring is provided at a position where the access door 15 is attached to the access port 14. When the transfer door 12 and the access door 15 are in a closed state, the sealing ring plays a role in blocking the circulation of internal and external gases, and improves the replacement efficiency of the precooling assembly 7 on the gas in the transfer window body 1.
As shown in fig. 6 and 10, the pre-cooling assembly 7 includes a gas dispersing member 71 and a joint 72, one end of the joint 72 is communicated with an external cooling gas source through a control valve, the other end of the joint 72 is communicated with a housing 711 of the gas dispersing member 71, and a plurality of nozzles 712 are arranged on the side surface of the housing 711. The cooling gas rapidly enters the transmission window body 1 through the nozzles 712 to carry out gas replacement, the nozzles 712 are arrayed on the housing 711 towards the synchronous belt transmission mechanism 4, the gas replacement efficiency is improved, and the cooling gas supplied by the precooling assembly 7 is water vapor-free cooling gas.
The external cooling gas is provided by the air precooler, the control valve is electrically connected with the controller, and the action of the control valve is controlled by the controller so as to control the on-off of the cooling gas flowing to the gas dispersing piece 71; the cooling gas is used for replacing the gas entering the internal environment of the transfer window body 1 due to the opening of the transfer door 12 or the access door 15, so that the internal temperature and humidity are kept constant, and the sample in the transfer tank is always in the constant temperature and humidity environment meeting the requirements in the transfer process;
as shown in fig. 4 and 11, the jacking component 8 includes two sleeved guide sleeves 81 and guide rods 82, the two guide sleeves 81 are connected with the bottom plate 13, the upper ends of the two guide rods 82 penetrate through the bottom plate 13 and are connected through a jacking table 83, the lower ends of the two guide rods 82 are connected through a connecting plate 84, the connecting plate 84 is connected with one end of a jacking piece 85, the other end of the jacking piece 85 is connected with the bottom plate 13, and a position sensor is arranged at the initial position of the up-and-down movement of the connecting plate 84. The jacking piece 85 is electrically connected with the controller, when the transfer pot is located above the jacking component 8, the controller controls the jacking piece 85 to stretch or shrink, the connecting plate 84 is driven to move downwards or upwards, the jacking table 83 is driven to move downwards or upwards through the guide rod 82, the jacking table 83 is made to abut against the transfer pot and drive the transfer pot to move upwards or downwards, and the transfer pot after jacking is beneficial to the operation of a robot for storing and taking samples into the transfer pot.
As shown in fig. 4 and 7-9, the transmission opening and closing mechanism 5 includes a push rod 51, one end of the push rod 51 is rotatably connected with the transmission door 12, the other end of the push rod 51 is rotatably connected with a screw rod nut assembly 53, the driving end of the screw rod nut assembly 53 is connected with a first motor 52, and a position sensor is arranged at the starting position of the movement of the transmission door 12.
The first motor 52 is electrically connected with the controller, and the controller controls the first motor 52 to rotate, so that the screw rod nut assembly 53 drives the push rod 51 to push or pull the transfer door 12 to swing around the rotating end, so as to open or close the transfer door 12, and when the transfer door 12 is closed, the transfer opening and closing mechanism 5 is located inside the transfer window body 1. The lead screw and nut assembly 53 is well known in the art and its structure and principles are well known to those skilled in the art and will not be described in detail herein.
The access opening and closing mechanism 6 comprises a rotating shaft 61 connected with the rotating end of the access door 15, the end part of the rotating shaft 61 is connected with a second motor 62, and a position sensor is arranged at the initial position of the movement of the access door 15. The access opening and closing mechanism 6 is installed on the outer side of the upper portion of the transmission window body 1, the second motor 62 is electrically connected with the controller, and the controller controls the second motor 62 to drive the rotating shaft 61 to rotate so as to drive the connected access door 15 to swing around the rotating end. The controller judges the open or closed state of the access door 15 or the transfer door 12 by a signal transmitted from the position sensor to judge the control operation to be performed.
A sample transfer method for a biological sample library is applied to a sample transfer device for the biological sample library, and comprises the following steps:
s1, when a biological sample is stored in a biological sample library, a robot conveys a transfer tank to the side of a transfer port 11 of a transfer window body 1, and a controller starts a precooling assembly 7 to replace gas in the transfer window body 1;
s2, controlling the transfer opening and closing mechanism 5 to open the transfer door 12, driving the front end of the mobile platform 2 to move out of the transfer port 11 by the driving assembly 3, placing the transfer tank on the front end synchronous belt conveying mechanism 4 of the mobile platform 2 by the robot, reversely moving and resetting the mobile platform 2, and resetting the transfer door 12;
s3, the synchronous belt conveying mechanism 4 drives the transfer tank to move below the access port 14;
s4, the controller starts the jacking component 8 to drive the top of the transfer tank to be flush with the access port 14, and when the temperature and humidity detection device detects that the temperature and humidity in the transfer window body 1 reach the required range, the precooling component 7 stops working;
s5, controlling the access opening and closing mechanism 6 to open the access door 15, taking a transfer tank cover by a robot, taking samples in the transfer tank, resetting the tank cover, resetting the access door 15, and resetting the jacking component 8;
s6, the controller starts the pre-cooling assembly 7 to replace the gas in the transmission window body 1;
s7, controlling the transfer opening and closing mechanism 5 to open the transfer door 12, driving the transfer tank to move to the front end of the mobile platform 2 by the synchronous belt conveying mechanism 4, driving the front end of the mobile platform 2 to move out of the transfer port 11 by the driving component 3, taking the transfer tank away by the robot, resetting the mobile platform 2, and resetting the transfer door 12;
and S8, when the temperature and humidity detection device detects that the temperature and humidity in the transmission window body 1 reaches the required range, the precooling assembly 7 stops working. The transfer of the sample to the biological sample storage repository is completed so far.
In step S5, after the robot takes the cover of the transfer tank, the liquid nitrogen is filled into the transfer tank through the liquid nitrogen filling mechanism at the top of the transfer window 1, and then the sample in the transfer tank is taken, and the cover is reset.
When the biological sample is transferred out of the biological sample library, the steps of the transfer method are basically the same as the operation of storing the biological sample into the biological sample library, and the difference is that: in step S5, the access opening and closing mechanism 6 is controlled to open the access door 15, the robot takes away the tank cover of the transfer tank, after filling liquid nitrogen into the transfer tank through the liquid nitrogen filling mechanism at the top of the transfer window 1, the sample is placed into the transfer tank, the tank cover is reset, the access door 15 is reset, and the jacking component 8 is reset.
In the process of storing or transferring biological samples into a biological sample library, in order to enable the samples to be in a specified environment during waiting to be taken away, after a robot takes away a cover of a transfer tank, the temperature and the humidity of the environment where the samples are located are always kept constant by filling liquid nitrogen into the transfer tank, so that the influence of environmental changes on the samples in the transfer process is effectively reduced.
In the description of the present invention, the terms "upper", "lower", "front", "rear", "left", "right", "top", "bottom", "vertical", "horizontal", etc. refer to the orientation or positional relationship based on that shown in the drawings, and are merely for the purpose of describing the present invention and do not require that the present invention must be constructed or operated in a specific orientation, and thus should not be construed as limiting the present invention. "connected" and "connected" in the present invention are to be understood broadly, and may be, for example, connected or detachably connected; the connection may be direct or indirect through intermediate members, and the specific meaning of the terms may be understood in detail by those skilled in the art.
The foregoing has been described in what is considered to be the preferred embodiments of the invention, and the description of specific examples is only intended to provide a better understanding of the principles of the invention. It will be apparent to those skilled in the art that modifications and equivalents may be made in accordance with the principles of the invention, and such modifications and equivalents are considered to fall within the scope of the invention.

Claims (9)

1. The sample transfer device for the biological sample library comprises a transfer window body (1) and a controller, wherein the transfer window body (1) is connected with a moving platform (2) in a sliding manner, a driving assembly (3) is arranged on the lower side of the moving platform (2), a synchronous belt transfer mechanism (4) for driving a transfer tank to translate is arranged on the moving platform (2), and the sample transfer device is characterized in that a transfer port (11) is arranged at the front part of the transfer window body (1), the transfer port (11) is hinged with a transfer door (12), the transfer door (12) is connected with a transfer opening and closing mechanism (5), the moving platform (2) penetrates through the transfer port (11) and is connected onto a bottom plate (13) of the transfer window body (1) in a sliding manner along the front-back direction, a liquid nitrogen filling mechanism and an access port (14) are arranged on the upper part of the transfer window body (1), the access port (14) is hinged with an access door (15), and the access door (15) is connected with an access opening and closing mechanism (6).
The cooling device is characterized in that a precooling assembly (7) for supplying cooling gas, a temperature and humidity detection device and a jacking assembly (8) for driving the transfer tank are arranged in the transfer window body (1), the jacking assembly (8) corresponds to the access port (14) up and down, and the driving assembly (3), the synchronous belt conveying mechanism (4), the transfer opening and closing mechanism (5), the access opening and closing mechanism (6), the precooling assembly (7) and the jacking assembly (8) are all electrically connected with the controller.
2. The sample transfer apparatus for a biological sample library according to claim 1, wherein a sealing ring is provided at a position where the transfer door (12) is attached to the transfer port (11), and a sealing ring is provided at a position where the access door (15) is attached to the access port (14).
3. A sample transfer apparatus for a biological sample library according to claim 1, wherein the pre-cooling assembly (7) comprises a gas dispersing member (71), a connector (72) and a control valve, one end of the connector (72) is connected to the gas dispersing member (71), the other end is connected to the control valve, the control valve is communicated with an external cooling air source, the gas dispersing member (71) comprises a housing (711), and a plurality of nozzles (712) are arranged on the side surface of the housing (711).
4. A sample transfer apparatus for biological sample libraries as claimed in claim 3, wherein the gas supplied by the pre-cooling assembly (7) is a moisture free cooling gas.
5. The sample transfer apparatus for a biological sample library according to claim 1, wherein the jacking component (8) comprises two sleeved guide sleeves (81) and guide rods (82), the two guide sleeves (81) are connected with the bottom plate (13), the upper ends of the two guide rods (82) penetrate through the bottom plate (13) and are connected through the jacking table (83), the lower ends of the two guide rods (82) are connected through a connecting plate (84), the connecting plate (84) is connected with one end of the jacking piece (85), the other end of the jacking piece (85) is connected with the bottom plate (13), and a position sensor is arranged at the initial position of the up-down movement of the connecting plate (84).
6. The sample transfer apparatus for a biological sample library according to claim 1, wherein the transfer opening and closing mechanism (5) comprises a push rod (51), one end of the push rod (51) is rotatably connected with the transfer door (12), the other end of the push rod (51) is rotatably connected with a screw rod screw assembly (53), the driving end of the screw rod screw assembly (53) is connected with a first motor (52), and a position sensor is arranged at the starting position of the movement of the transfer door (12).
7. A sample transfer apparatus for a biological sample library according to claim 1, wherein the access opening and closing mechanism (6) comprises a rotating shaft (61) connected to a rotating end of the access door (15), an end of the rotating shaft (61) is connected to a second motor (62), and a position sensor is provided at a starting position of the movement of the access door (15).
8. A sample transfer method for a biological sample library, applied to the sample transfer apparatus for a biological sample library according to claim 1, comprising the steps of:
s1, when a biological sample is stored in a biological sample library, a robot conveys a transfer tank to the side of a transfer port (11) of a transfer window (1), and a controller starts a precooling assembly (7) to replace gas in the transfer window (1);
s2, controlling a transfer opening and closing mechanism (5) to open a transfer door (12), driving the front end of a mobile platform (2) to move out of a transfer port (11) by a driving assembly (3), placing a transfer tank on a synchronous belt conveying mechanism (4) at the front end of the mobile platform (2) by a robot, reversely moving and resetting the mobile platform (2), and resetting the transfer door (12);
s3, driving the transfer tank to move below the access port (14) by the synchronous belt conveying mechanism (4);
s4, the controller starts the jacking component (8) to drive the top of the transfer pot to be level with the access port (14), and when the temperature and humidity detection device detects that the temperature and humidity in the transmission window body (1) reach the required range, the precooling component (7) stops working;
s5, controlling an access opening and closing mechanism (6) to open an access door (15), taking a transfer tank cover by a robot, taking samples in the transfer tank, resetting the tank cover, resetting the access door (15), and resetting a jacking component (8);
s6, the controller starts the pre-cooling assembly (7) to replace gas in the transmission window body (1);
s7, controlling a transfer opening and closing mechanism (5) to open a transfer door (12), driving a transfer tank to move to the front end of a mobile platform (2) by a synchronous belt conveying mechanism (4), driving the front end of the mobile platform (2) to move out of a transfer port (11) by a driving assembly (3), taking the transfer tank away by a robot, resetting the mobile platform (2), and resetting the transfer door (12);
s8, when the temperature and humidity detection device detects that the temperature and humidity in the transmission window body (1) reach the required range, the precooling assembly (7) stops working.
9. The sample transfer method for biological sample library according to claim 8, wherein in step 5, after the robot takes the transfer can lid, liquid nitrogen is filled into the transfer can through the liquid nitrogen filling mechanism at the top of the transfer window (1), and then the sample in the transfer can is taken again, and the can lid is reset.
CN202310275545.2A 2023-03-21 2023-03-21 Sample transfer device for biological sample library and transfer method thereof Pending CN116040191A (en)

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