CN220251501U - Processing box for experimental sample of modification group - Google Patents

Abstract

The utility model discloses a modified histology experiment sample treatment box, which comprises a box body with a rectangular structure, wherein a pair of operation holes with sealing doors are formed in the front wall of the box body, flexible protective sleeves are connected to the orifices of the operation holes, a transport box jack is formed in the front wall of the box body below the operation holes, and flexible sealing blocks are arranged in the orifices of the transport box jack; the top wall of the box body is provided with a nitrogen blowing pipe, the lower edges of the left side wall and the right side wall of the box body are provided with one-way exhaust ports, the box body is internally provided with a lifting plate and a rotating plate, and the outer wall of the box body is provided with a first power source and a second power source; the lifting plate is driven by a first power source to have a first position propped against the top wall of the box body and a second position propped against the bottom wall of the box body, and the rotating plate is driven by a second power source to have a vertical position propped against the front wall of the box body and a horizontal position flush with the lower edge of the jack of the transfer box. The utility model has the advantages of greatly reducing the air content in the box body, effectively improving the concentration of nitrogen introduced into the box body and improving the effect of sample pretreatment.

Description

Processing box for experimental sample of modification group

Technical Field

The utility model relates to the technical field of modification histology experiments, in particular to a modification histology experiment sample treatment box.

Background

Modification histology is a hotspot in the current basic research that is the alteration of protein function by studying modifications on different proteins, for example: in general, proteins are activated after phosphorylation to perform their physiological functions, and protein degradation occurs after ubiquitination, and there are various types of glycosylation, acetylation, and the like.

The method for detecting the mass spectrum is required to be applied in the research and modification of the group, the sample is required to be treated before the mass spectrum, and the sample is required to be isolated from air during the treatment, so that the nitrogen is not easy to react with amino acid residues on protein, the influence on the result can be well avoided, and the sample is mostly placed in a transport box with an open structure and then placed in a nitrogen box for treatment. However, there is no nitrogen box for modification group experiments on the market at present, usually the reagent or the sample and the transfer box are placed in a closed box body together, then nitrogen is filled into the box body, but the air in the box body can only be mixed with the nitrogen by directly filling the nitrogen, and the nitrogen concentration can not be obviously improved.

Disclosure of Invention

The utility model aims to provide a processing box for a modified histology experiment sample.

In order to achieve the above purpose, the present utility model may adopt the following technical scheme:

the utility model relates to a modified histology experiment sample treatment box, which comprises a box body with a rectangular structure, wherein a pair of operation holes with sealing doors are formed in the front wall of the box body, flexible protective sleeves are connected to the orifices of the operation holes, a transport box jack is formed in the front wall of the box body below the operation holes, and flexible sealing blocks are arranged in the orifices of the transport box jack; a nitrogen blowing pipe is arranged on the top wall of the box body, unidirectional exhaust ports are arranged at the lower edges of the left side wall and the right side wall of the box body, a lifting plate and a rotating plate are arranged in the box body, and a first power source and a second power source are arranged on the outer wall of the box body; the lifting plate is driven by the first power source to move upwards to a first position propped against the top wall of the box body and move downwards to a second position propped against the bottom wall of the box body, the rotating plate is driven by the second power source to rotate upwards to a vertical position propped against the front wall of the box body and rotate downwards to a horizontal position flush with the lower edge of the jack of the transfer box, the front edge of the lifting plate props against the upper edge of the rotating plate located at the vertical position when the lifting plate is located at the first position, and the lower surface of the rotating plate is attached to the upper surface of the lifting plate located at the second position when the rotating plate is located at the horizontal position.

The utility model has the advantages that when nitrogen is introduced into the box body, the downward moving lifting plate can be utilized to squeeze air in the box body, and the operation hole and the transfer box jack are further effectively blocked by matching with the rotating plate at the vertical position, so that the air can be discharged through the unidirectional exhaust port as much as possible, the unidirectional exhaust port can be easily blocked when the lifting plate moves to the second position, the air content in the box body is greatly reduced, the nitrogen concentration introduced into the box body is effectively improved, the sample pretreatment effect is improved, and the mass spectrum detection accuracy of the modification group is improved.

Further, the first power source comprises a first sealed shell arranged on the rear wall of the box body, a screw rod with the same height as the box body and a first motor for driving the screw rod to rotate are vertically arranged in the first sealed shell, a vertical sliding groove corresponding to the screw rod in an adapting mode is formed in the rear wall of the box body positioned on the inner side of the first sealed shell, a sliding block horizontally penetrating through the vertical sliding groove is connected to the lifting plate, and a follower nut adapting to the screw rod in a screwed mode is connected to the sliding block; the screw rod is driven to rotate through the first motor, so that the follower nut sleeved on the screw rod can move up and down easily under the action of the matching block of the sliding block and the vertical sliding groove, the lifting plate is driven to move up and down by the first motor, and the conversion between the first position and the second position of the lifting plate is realized easily.

Further, the second power source comprises a second sealed shell arranged on the right side wall of the box body, a second motor is arranged in the second sealed shell, and a driving shaft of the second motor horizontally penetrates through the right side wall of the box body and is vertically and fixedly connected to the right edge of the rotating plate; the second motor drives the rotating plate to rotate, so that the conversion between the vertical position and the horizontal position of the rotating plate is easily realized.

Further, for convenient processing and subsequent use, the sealing door is a rotary door plate hinged on the front wall of the box body, and the rotary door plate is clung to the front wall of the box body and is provided with a closed position for being buckled on the operation hole in a sealing way and an open position for being buckled above the operation hole in a rotating way.

Further, the flexible sealing block is a rubber sealing block or a silica gel sealing block which is matched with and covers the transport box jack, the upper edge of the flexible sealing block is fixedly connected to the upper edge of the orifice of the transport box jack, and the lower edge of the flexible sealing block is lapped on the lower edge of the orifice of the transport box jack; the box jack is transported to the convenience in pushing into the box in transporting the box to can be when transporting the box and extract and transport the box jack outward, utilize flexible fender self elasticity to rebound the closing cap to transport the box jack, reduce the air through transporting in the box jack gets into the box as far as possible, promote the nitrogen gas concentration that fills in the box.

Further, the nitrogen blowing pipe is composed of a blowing main pipe, a first blowing branch pipe and a second blowing branch pipe, wherein the first blowing branch pipe and the second blowing branch pipe are communicated with the blowing main pipe through a three-way joint, the air outlet end of the first blowing branch pipe is connected to the top wall of the box body, the air outlet end of the second blowing branch pipe passes through the top wall of the box body in a sealing way and extends into the inner cavity of the box body, and control valves are respectively arranged on the first blowing branch pipe and the second blowing branch pipe; the first air blowing branch pipe can continuously blow nitrogen into the box body, and the second air blowing branch pipe can be used for facilitating an operator to directly blow nitrogen to the sample in a short distance in actual operation, so that air in the sample is rapidly discharged.

Further, at least two groups of tightening belts are arranged on the flexible protective sleeve at intervals along the length direction; after the two arms of an operator extend into the box body through the flexible protective sleeve, the flexible protective sleeve can be fastened, so that the normal operation of the operator is ensured, and air can be reduced to enter the box body through a gap of the flexible protective sleeve.

Drawings

Fig. 1 is a schematic structural view of the present utility model.

Fig. 2 is a top view (hidden nitrogen purge blowing pipe) of fig. 1.

Fig. 3 is a state diagram of an initial station of the present utility model.

Fig. 4 is a final station state diagram of the present utility model.

Detailed Description

The following description of the embodiments of the present utility model will be made clearly and fully with reference to the accompanying drawings, in which it is evident that the embodiments described are only some, but not all embodiments of the utility model. All other embodiments, which can be made by those skilled in the art based on the embodiments of the utility model without making any inventive effort, are intended to be within the scope of the utility model.

It should be noted that all directional indicators (such as up, down, left, right, front, and rear … …) in the embodiments of the present utility model are merely used to explain the relative positional relationship, movement, etc. between the components in a particular posture (as shown in the drawings), and if the particular posture is changed, the directional indicator is changed accordingly.

In the present utility model, unless specifically stated and limited otherwise, the terms "connected," "affixed," and the like are to be construed broadly, and for example, "affixed" may be a fixed connection, a removable connection, or an integral body; can be mechanically or electrically connected; either directly or indirectly, through intermediaries, or both, may be in communication with each other or in interaction with each other, unless expressly defined otherwise. The specific meaning of the above terms in the present utility model can be understood by those of ordinary skill in the art according to the specific circumstances.

Furthermore, descriptions such as those referred to as "first," "second," and the like, are provided for descriptive purposes only and are not to be construed as indicating or implying a relative importance or implying an order of magnitude of the indicated technical features in the present disclosure. Thus, a feature defining "a first" or "a second" may explicitly or implicitly include at least one such feature.

As shown in fig. 1 to 4, the treatment box for modified laboratory samples according to the present utility model comprises a rectangular box 1, wherein a pair of operation holes 3 with sealing doors 2 are formed in the front wall of the box 1, and for convenience in processing, manufacturing and subsequent use, the sealing doors 2 may be specifically a rotary door plate hinged to the front wall of the box 1, and the rotary door plate is tightly attached to the front wall of the box 1 and has a closed position sealed on the operation holes 3 and an open position rotated above the operation holes 3. In addition, the inner edge of the hole opening of the operation hole 3 is also connected with a flexible protective sleeve 4 extending into the box body 1, and at least two groups of tightening belts 5 are arranged on the flexible protective sleeve 4 at intervals along the length direction, wherein one group of tightening belts 5 is positioned on the inner port edge of the flexible protective sleeve 4; the flexible protection oversleeve 4 can be convenient for the operating personnel hand stretch into the box 1 and operate, and tie up the area 5 and be the elasticity clitellum that the ring was fixed in on the flexible protection oversleeve 4, when operating personnel's both arms stretch into the box 1 through flexible protection oversleeve 4, can utilize the elasticity of tie up area 5 to tie up flexible protection oversleeve 4, and then guarantee operating personnel normal operating in, can effectively reduce the air and get into in the box 1 through the gap of flexible protection oversleeve 4.

The box 1 front wall that is located the operation hole 3 below has been seted up and has been transported box jack 6, the size of this box jack 6 should be with the current size looks adaptation that uses the conventional box of transporting when decorating the experimental sample preliminary treatment of group, thereby can guarantee to transport the box and easily insert in the box 1 through transporting box jack 6, of course still can set up one deck flexible rubber bed course or flexible silica gel bed course on transporting box jack 6's pore wall, thereby when transporting the box and inserting and transporting box jack 6 in, can make and transport the box jack 6 between laminating inseparabler, promote the leakproofness between the two, reduce the air through transporting box jack 6 entering box 1 as far as possible. In addition, still can set up a flexible fender 7 in the drill way of transporting box jack 6, this flexible fender 7 can be the rubber separation blade or the silica gel separation blade of adaptation closing cap transport box jack 6, the upper edge of flexible fender 7 links firmly on transporting box jack 6's drill way upper edge, the lower edge of flexible fender 7 then overlap joint is on transporting box jack 6's drill way lower edge, thereby form the closing cap to transporting box jack 6, and when transporting box jack 6 and pushing into the transport box, transport the box and can easily push up flexible fender 7, ensure to transport the box and can easily push into box 1 in, simultaneously can also utilize flexible fender 7's self elasticity and automatic resilience when transporting the box and ejecting out of transporting box jack 6, transport box jack 6 is sealed again, further reduction air gets into the box through transporting box jack 6, promote the inside seal of box 1.

A nitrogen blowing pipe is provided on the top wall of the tank 1, specifically, the nitrogen blowing pipe is composed of a main blowing pipe 8, and a first blowing branch pipe 9 and a second blowing branch pipe 10 which are communicated with the main blowing pipe 8 through a three-way joint, and control valves 11 are provided on the first blowing branch pipe 9 and the second blowing branch pipe 10, respectively. The air blowing main pipe 8, the first air blowing branch pipe 9 and the second air blowing branch pipe 10 are flexible pipes, and the air outlet end of the first air blowing branch pipe 9 is fixedly connected to the top wall of the box body 1 so as to be communicated with the inner cavity of the box body 1, and can continuously blow nitrogen into the box body 1; the air outlet end of the second air blowing branch pipe 10 passes through the top wall of the box body 1 in a sealing way and extends into the inner cavity of the box body, so that hands of operators can extend into the box body 1 to operate the second air blowing branch pipe 10 in actual operation, and then nitrogen can be blown to a sample in a direct short distance, and the aim of rapidly discharging air in the sample is achieved.

The lower edges of the left side wall and the right side wall of the box body 1 are provided with one-way exhaust ports 12, a lifting plate 13 and a rotating plate 14 are arranged in the box body 1, and a first power source for driving the lifting plate 13 to move and a second power source for driving the rotating plate 14 to move are arranged on the outer wall of the box body 1; at this time, the lifting plate 13 is driven by the first power source to have a first position to move upward to be abutted against the top wall of the case 1 and a second position to move downward to be abutted against the bottom wall of the case 1, and the rotating plate 14 is driven by the second power source to have a vertical position to rotate upward to be abutted against the front wall of the case 1 and a horizontal position to be flush with the lower edge of the transfer box insertion hole 6. Specifically, the first power source includes the first sealed shell 15 that sets up on the back wall of box 1, vertically be provided with in the first sealed shell 15 with the lead screw 16 of box 1 highly uniform and drive the first motor 17 of lead screw 16 rotation, at this moment, set up the vertical spout that corresponds with the lead screw 16 adaptation on the box 1 back wall of first sealed shell 15 inboard, be connected with the slider 18 that passes from the vertical spout level on lifter plate 13, be connected with the follower nut 19 of adaptation spiro union on lead screw 16 on the slider 18, thereby can drive the lead screw 16 rotation through first motor 17, and then make the follower nut 19 that is overlapped on lead screw 16 follow the lead screw 16 length direction and reciprocate under the cooperation blocking effect of slider 18 and vertical spout, thereby realize driving lifter plate 13 by first motor 17 and reciprocate, easily realize lifter plate 13 conversion between first position and second position. The second power source comprises a second sealed shell 20 arranged on the right side wall of the box body 1, a second motor 21 is arranged in the second sealed shell 20, and a driving shaft of the second motor 21 horizontally penetrates through the right side wall of the box body 1 and is vertically and fixedly connected to the upper corner of the right edge of the rotating plate 14, so that the rotating plate 14 can be driven to rotate through the second motor 21, and the conversion of the rotating plate 14 between the vertical position and the horizontal position can be easily realized.

In addition, in the process of the back and forth movement of the lifting plate 13 between the first position and the second position, the left side edge, the right side edge and the rear edge of the lifting plate should be always attached to the left side wall, the right side wall and the rear wall of the box body 1, and in the process of the back and forth movement of the rotating plate 14 between the vertical position and the horizontal position, the left side edge and the right side edge of the lifting plate should be always attached to the left side wall and the right side wall of the box body 1; meanwhile, when the lifting plate 13 is positioned at the first position, the front edge of the lifting plate 13 just props against the upper edge of the rotating plate 14 positioned at the vertical position, when the lifting plate 13 is positioned at the second position, the left and right side edges of the lifting plate just block the unidirectional exhaust ports 12 on the left and right side walls of the box body 1, when the rotating plate 14 is positioned at the vertical position, the upper surface of the lifting plate is just jointed and buckled on the upper surface of the lifting plate 13 positioned at the second position when the rotating plate 14 is positioned at the horizontal position, and the upper surface of the lifting plate is just jointed and buckled on the upper surface of the cover operation hole 3 and the transfer box jack 6 on the front arm of the box body 1.

When the device is used, nitrogen is continuously blown into the box body 1 through the first blowing branch pipe 9 by the blowing main pipe 8, the lifting plate 13 can be driven to move from the first position to the second position by the first power source in the process of blowing the nitrogen, the lifting plate 13 can extrude air in the box body 1 downwards as much as possible in the moving process and discharge the air out of the box body 1 through the unidirectional exhaust port 12, meanwhile, the rotating plate 14 is always in the vertical position in the process, and the operation hole 3 and the transfer box jack 6 can be effectively blocked, so that the air content in the box body 1 is greatly reduced, and the nitrogen concentration introduced into the box body 1 is effectively improved; and then the rotating plate 14 is driven to move from the vertical position to the horizontal position by the second power source, so that the sample can be pretreated by inserting the transfer box into the transfer box jack 6, the effect of sample pretreatment is effectively improved, and the accuracy of mass spectrum detection in subsequent modification group is improved.

Claims (7)

1. A modified histology experiment sample treatment box which characterized in that: the box comprises a box body with a rectangular structure, wherein a pair of operation holes with sealing doors are formed in the front wall of the box body, flexible protection oversleeves are connected to the orifice of each operation hole, a transfer box jack is formed in the front wall of the box body below each operation hole, and flexible sealing blocks are arranged in the orifice of each transfer box jack; a nitrogen blowing pipe is arranged on the top wall of the box body, unidirectional exhaust ports are arranged at the lower edges of the left side wall and the right side wall of the box body, a lifting plate and a rotating plate are arranged in the box body, and a first power source and a second power source are arranged on the outer wall of the box body; the lifting plate is driven by the first power source to move upwards to a first position propped against the top wall of the box body and move downwards to a second position propped against the bottom wall of the box body, the rotating plate is driven by the second power source to rotate upwards to a vertical position propped against the front wall of the box body and rotate downwards to a horizontal position flush with the lower edge of the jack of the transfer box, the front edge of the lifting plate props against the upper edge of the rotating plate located at the vertical position when the lifting plate is located at the first position, and the lower surface of the rotating plate is attached to the upper surface of the lifting plate located at the second position when the rotating plate is located at the horizontal position.

2. The modified histology experiment sample processing kit of claim 1, wherein: the first power source is arranged on the rear wall of the box body, a screw rod with the height being identical to that of the box body and a first motor for driving the screw rod to rotate are vertically arranged in the first sealed housing, a vertical sliding groove corresponding to the screw rod in an adaptation mode is formed in the rear wall of the box body on the inner side of the first sealed housing, a sliding block penetrating through the vertical sliding groove horizontally is connected to the lifting plate, and an adaptation screw joint is connected to the sliding block through a follow-up nut on the screw rod.

3. The modified histology experiment sample processing kit of claim 1, wherein: the second power source comprises a second sealing shell arranged on the right side wall of the box body, a second motor is arranged in the second sealing shell, and a driving shaft of the second motor horizontally penetrates through the right side wall of the box body and is vertically and fixedly connected to the right edge of the rotating plate.

4. The modified histology experiment sample processing kit of claim 1, wherein: the sealing door is a rotary door plate hinged on the front wall of the box body, and the rotary door plate is clung to the front wall of the box body and is provided with a closed position for rotating to be sealed and buckled on the operation hole and an open position for rotating to be above the operation hole.

5. The modified histology experiment sample processing kit of claim 1, wherein: the flexible sealing block is a rubber baffle or a silica gel baffle which is matched with the sealing cover of the transport box jack, the upper edge of the flexible sealing block is fixedly connected to the upper edge of the orifice of the transport box jack, and the lower edge of the flexible sealing block is lapped on the lower edge of the orifice of the transport box jack.

6. The modified histology experiment sample processing kit of claim 1, wherein: the nitrogen blowing pipe is composed of a blowing main pipe, a first blowing branch pipe and a second blowing branch pipe, wherein the first blowing branch pipe and the second blowing branch pipe are communicated with the blowing main pipe through a three-way joint, the air outlet end of the first blowing branch pipe is connected to the top wall of the box body, the air outlet end of the second blowing branch pipe penetrates through the top wall of the box body in a sealing mode and extends into the inner cavity of the box body, and control valves are respectively arranged on the first blowing branch pipe and the second blowing branch pipe.

7. The modified histology experiment sample processing kit of claim 1, wherein: at least two groups of tightening belts are arranged on the flexible protective oversleeve at intervals along the length direction.