CN114852387A

CN114852387A - Trace oligonucleotide synthesis filling device and method

Info

Publication number: CN114852387A
Application number: CN202210643094.9A
Authority: CN
Inventors: 茆辉; 陈鹏
Original assignee: Nanjing Jisheng Aoma Biomedical Co ltd
Current assignee: Nanjing Jisheng Aoma Biomedical Co ltd
Priority date: 2022-06-08
Filing date: 2022-06-08
Publication date: 2022-08-05

Abstract

The invention discloses a trace oligonucleotide synthesis filling device and a method, relating to the technical field of nucleotide extraction and aiming at providing a trace oligonucleotide synthesis filling device and a method for realizing automatic opening and closing of an injection channel in a reagent tube, simplifying the reagent filling steps and improving the reagent filling efficiency, and the technical key points are that the reagent tube comprises a liquid filling tube section, the top end of the liquid filling tube section is provided with a liquid inlet tube section, the top of the liquid inlet tube section is provided with a liquid inlet, an injection channel is formed below the liquid inlet, a sealing plate is movably arranged in the inner cavity of the liquid inlet tube section, the injection tube passes through the liquid inlet to push the sealing plate to move to open the injection channel, and the sealing plate returns to the original position to seal the injection channel after the injection tube leaves the liquid inlet tube section, the technical effect is that the shape of the reagent tube is limited, the device comprises the liquid filling tube section and the liquid inlet tube section, and the injection channel in the liquid inlet tube section is sealed by the sealing plate, the whole device realizes the automatic opening and closing of the injection channel, simplifies the reagent filling steps and improves the efficiency.

Description

Trace oligonucleotide synthesis filling device and method

Technical Field

The invention relates to the technical field of nucleotide extraction, in particular to a trace oligonucleotide synthesis filling device and a trace oligonucleotide synthesis filling method.

Background

Oligonucleotides play an important role in the field of modern life sciences, generally refer to linear polynucleotide fragments formed by connecting nucleotide residues by phosphodiester bonds, can be automatically synthesized by an instrument, and mainly play a role in serving as primers, gene probes and the like for DNA synthesis.

In the synthetic process of oligonucleotide, need extract the nucleic acid reagent to the deep-hole board that is equipped with the lysate in, later put into the nucleic acid with the deep-hole board and carry out automation mechanized operation in the appearance, and to the filling of nucleic acid extraction reagent, but it is comparatively loaded down with trivial details among the prior art, because the structure of reagent pipe generally includes body and lid two parts, generally connect as an organic wholely through plastics connecting portion between lid and the body, when the automatic filling of reagent, need carry out material loading, filling, four steps of membrane sealing and unloading, reagent filling efficiency has been reduced.

Disclosure of Invention

Technical problem to be solved

Aiming at the defects of the prior art, the invention provides a trace oligonucleotide synthesis filling device and method for realizing automatic opening and closing of an injection channel in a reagent tube, simplifying the reagent filling steps and improving the reagent filling efficiency.

(II) technical scheme

In order to achieve the purpose, the invention provides the following technical scheme: a trace oligonucleotide synthesis filling device comprises a workbench, wherein a rotating table is mounted on the workbench, the power source of the rotating table is a driving motor, a feeding area, a filling area and a discharging area are arranged on the rotating table, object placing plates are mounted on the feeding area, the filling area and the discharging area and used for placing reagent tubes, a feeding mechanism is arranged above the feeding area, a filling mechanism is arranged above the filling area, and a discharging mechanism is arranged above the discharging area;

the reagent tube feeding device comprises a worktable, a reagent tube, a feeding mechanism, a vibrating disk, a feeding mechanism and a discharging mechanism, wherein the worktable is also provided with the vibrating disk, the outer part of the vibrating disk is provided with a feeding track, the vibrating disk sequentially discharges the reagent tubes onto the feeding track, the feeding track is used for conveying materials, and the feeding mechanism is used for extracting the materials of the feeding track to a feeding area;

the feeding mechanism comprises a lifting part and a transmission part, the lifting part is used for extracting the reagent tube and driving the reagent tube to move along the vertical direction, and the transmission part is used for driving the lifting part to move along the horizontal direction;

the filling mechanism comprises a lifting part, a filling part and a liquid medicine tank, the liquid medicine tank is connected with the filling part through a conveying pipe, the lifting part is used for driving the filling part to ascend and descend, a plurality of injection tubes are arranged on the filling part, the injection tubes are communicated with the conveying pipe, and the injection tubes are used for injecting reagents into the reagent tubes;

a material receiving box is further installed on the outer side of the workbench, a discharging slideway is arranged between the material receiving box and the rotating platform, the discharging mechanism is used for extracting materials in a discharging area onto the discharging slideway, and the materials enter the material receiving box along the discharging slideway;

the blanking mechanism comprises a clamping part and a driving part, the clamping part is used for clamping the reagent tube and driving the reagent tube to move along the vertical direction, and the driving part is used for driving the clamping part to move along the horizontal direction;

the reagent tube comprises a liquid filling tube section, a liquid inlet tube section is arranged at the top end of the liquid filling tube section, a liquid inlet is formed in the top of the liquid inlet tube section, an injection channel is formed below the liquid inlet, a sealing plate is movably mounted in an inner cavity of the liquid inlet tube section, the injection tube penetrates through the liquid inlet to push the sealing plate to move to open the injection channel, and after the injection tube leaves the liquid inlet tube section, the sealing plate returns to the original position to seal the injection channel.

Preferably, the workbench is provided with a mounting frame plate, the transmission part and the driving part are the same mechanism, the transmission part comprises a driving cylinder and a fixed rod, the telescopic end of the driving cylinder is provided with a transmission rod, the transmission rod is sleeved with the fixed rod in a sliding manner outside the fixed rod, and the material lifting part and the clamping part are fixedly mounted on the transmission rod;

the material lifting part and the clamping part respectively comprise a lifting cylinder and a fixed block, two clamping plates and two power cylinders are installed at the bottom of the fixed block in a sliding mode, and the power cylinders drive the two clamping plates to move relatively.

Preferably, the rubber layer is all installed to the opposite side of grip block, offers the mounting groove with reagent pipe looks adaptation on the rubber layer.

Preferably, the inner cavity of the liquid inlet pipe section is rotatably provided with a rotating rod, a sealing plate is fixedly arranged on the rotating rod, and the diameter of the sealing plate is matched with the inner diameter of the liquid inlet pipe section;

the sealing plate comprises a gravity part and a light plate part, the weight of the light plate part is lower than that of the gravity part, and the injection tube penetrates through the liquid inlet to push the light plate part to rotate downwards and open the injection channel;

the inner wall of the liquid inlet pipe section is provided with a baffle block which is used for preventing the sealing plate from rotating under the action of gravity.

Preferably, the number of the baffle blocks is two, wherein the bottom surface of one baffle block is abutted against the top surface of the light plate portion, and the top surface of the other baffle block is abutted against the bottom surface of the gravity portion.

Preferably, the liquid inlet pipe section is arranged in a circular truncated cone shape, two sealing plates are oppositely arranged in an inner cavity of the liquid inlet pipe section, the tops of the sealing plates are fixedly connected with the top of the liquid inlet pipe section through reset springs, and the outer sides of the sealing plates are slidably connected with the inner wall of the liquid inlet pipe section;

the injection pipe penetrates through the liquid inlet to push the sealing plates to slide downwards along the inner wall of the liquid inlet pipe section, and the two sealing plates are separated to open an injection channel.

Preferably, be equipped with the hollow layer between the inner wall of feed liquor pipeline section and the outer wall, the inner chamber of hollow layer is equipped with the limiting plate, the inner wall laminating of limiting plate and hollow layer, the inner wall of feed liquor pipeline section offer with the long type hole of hollow layer intercommunication, the inside in long type hole is equipped with the connecting rod, the both ends of connecting rod respectively with limiting plate and closing plate connection, and the closing plate seals sealed hole.

A method for synthesizing and filling a trace amount of oligonucleotide is applied to the method for synthesizing and filling the trace amount of oligonucleotide, and comprises the following steps:

s1, pouring the reagent tubes into the vibrating disk, starting the vibrating disk, and discharging the reagent tubes to the feeding track in order;

s2, the reagent tube is driven to move by the feeding track, and the material of the feeding track is extracted to a feeding area by the feeding mechanism to finish feeding;

s3, the driving motor and the feeding mechanism run synchronously, the driving motor drives the rotating platform to rotate until the reagent tube rotates to the filling area, and meanwhile, the feeding mechanism returns to the original position, and the lifting part moves to the position above the feeding track;

s4, the feeding mechanism and the filling mechanism synchronously operate, the feeding mechanism extracts the materials of the feeding track to a feeding area, an injection tube of the filling mechanism injects the reagents into the reagent tube and returns to the original position, and feeding and filling are completed simultaneously;

s5, the driving motor and the feeding mechanism continue to run synchronously, the driving motor rotates until the filled reagent tube moves to the blanking area, the filled reagent tube moves to the filling area, and meanwhile, the feeding mechanism returns to the original position, and the lifting part moves to the upper part of the feeding track;

s6, the feeding mechanism, the filling mechanism and the discharging mechanism synchronously operate, and feeding, filling and discharging are completed simultaneously;

the reagent tube is lifted by a lifting part of the feeding mechanism until the transmission part drives the reagent tube to move to the feeding area, and the lifting part is separated from the reagent tube to finish feeding; the filling mechanism is provided with a lifting part and a sealing plate, wherein the lifting part drives the filling part to descend, the injection tube penetrates through the liquid inlet to push the sealing plate to move to open an injection channel, and the injection tube injects a reagent into the reagent tube and returns to the original position to finish filling; the clamping part of the blanking mechanism extracts the reagent tube until the driving part drives the reagent tube to move to the upper part of the blanking slideway, and the clamping part loosens the fixation of the reagent tube to complete blanking.

Preferably, in S5, when the feeding mechanism and the discharging mechanism are operated, the driving cylinder drives the driving rod to operate, and the driving rod drives the lifting member and the clamping member to move simultaneously.

(III) advantageous effects

Compared with the prior art, the invention provides a trace oligonucleotide synthesis filling device and a method, which have the following beneficial effects:

1. through the shape of injecing reagent pipe, including liquid loading pipeline section and feed liquor pipeline section, the injection passage in the feed liquor pipeline section is sealed by the closing plate, when the injection pipe passed the inlet and injects or draw reagent, promotes the closing plate motion and opens the injection passage, and when the injection pipe left the feed liquor pipeline section, the closing plate recovered the normal position and continues to seal the injection passage, and the automation of injection passage is realized opening and shutting to whole device to retrench the filling step of reagent, improve the filling efficiency of reagent.

2. Because the shape of the reagent tube is limited, the worker can directly insert the injection tube of the extractor into the reagent tube without manually opening the cover, and the extraction efficiency of the reagent is improved.

Drawings

FIG. 1 is a schematic front view of one embodiment of the present invention;

FIG. 2 is a schematic top view of one embodiment of the present invention;

FIG. 3 is a schematic diagram of the movement of a reagent tube in one embodiment of the present invention;

FIG. 4 is a schematic diagram of the movement of a reagent tube according to another embodiment of the present invention;

FIG. 5 is an enlarged view taken at A in FIG. 4 according to the present invention;

fig. 6 is a schematic view of one embodiment of the holding member of the present invention.

In the figure: 1. a work table; 2. a rotating table; 3. a drive motor; 4. a feeding area; 5. a filling area; 6. a connecting rod; 7. a blanking area; 8. a storage plate; 9. a reagent tube; 901. a liquid loading pipe section; 902. a liquid inlet pipe section; 10. a feeding mechanism; 1001. a material lifting component; 1002. a transmission member; 11. a filling mechanism; 1101. a lifting member; 1102. a filling component; 12. a blanking mechanism; 1201. a clamping member; 1202. a drive member; 13. vibrating the disc; 14. a feeding track; 15. a liquid medicine tank; 16. a delivery pipe; 17. an injection tube; 18. a material receiving box; 19. a blanking slideway; 20. a liquid inlet; 21. an injection channel; 22. a sealing plate; 2201. a gravity section; 2202. a light plate portion; 23. mounting a frame plate; 24. a driving cylinder; 25. fixing the rod; 26. a transmission rod; 27. a lifting cylinder; 28. a fixed block; 29. a clamping plate; 30. a power cylinder; 31. a rubber layer; 32. mounting grooves; 33. rotating the rod; 34. a baffle block; 35. a return spring; 36. a hollow layer; 37. a limiting plate; 38. an elongated hole.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the drawings in the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

Referring to fig. 1-6, a trace oligonucleotide synthesis filling device includes a workbench 1, a rotating table 2 is mounted on the workbench 1, a power source of the rotating table 2 is a driving motor 3, a feeding area 4, a filling area 5 and a discharging area 7 are arranged on the rotating table 2, object placing plates 8 are mounted on the feeding area 4, the filling area 5 and the discharging area 7, the object placing plates 8 are used for placing reagent tubes 9, a feeding mechanism 10 is arranged above the feeding area 4, a filling mechanism 11 is arranged above the filling area 5, and a discharging mechanism 12 is arranged above the discharging area 7;

the worktable 1 is also provided with a vibrating disk 13, a feeding track 14 is arranged outside the vibrating disk 13, the vibrating disk 13 orderly discharges the reagent tubes 9 onto the feeding track 14, the feeding track 14 is used for conveying materials, and the feeding mechanism 10 is used for extracting the materials of the feeding track 14 to the feeding area 4;

the feeding mechanism 10 comprises a lifting component 1001 and a transmission component 1002, wherein the lifting component 1001 is used for extracting the reagent tube 9 and driving the reagent tube to move along the vertical direction, and the transmission component 1002 is used for driving the lifting component 1001 to move along the horizontal direction;

the filling mechanism 11 comprises a lifting component 1101, a filling component 1102 and a liquid medicine tank 15, the liquid medicine tank 15 is connected with the filling component 1102 through a conveying pipe 16, the lifting component 1101 is used for driving the filling component 1102 to ascend and descend, the filling component 1102 is provided with a plurality of injection pipes 17, the injection pipes 17 are communicated with the conveying pipe 16, and the injection pipes 17 are used for injecting reagents into the reagent pipes 9;

a material receiving box 18 is further installed on the outer side of the workbench 1, a discharging slideway 19 is arranged between the material receiving box 18 and the rotating platform 2, the discharging mechanism 12 is used for extracting the material in the discharging area 7 onto the discharging slideway 19, and the material enters the material receiving box 18 along the discharging slideway 19;

the blanking mechanism 12 comprises a clamping part 1201 and a driving part 1202, wherein the clamping part 1201 is used for clamping the reagent tube 9 and driving the reagent tube to move along the vertical direction, and the driving part 1202 is used for driving the clamping part 1201 to move along the horizontal direction;

the reagent tube 9 comprises a liquid filling tube section 901, a liquid inlet tube section 902 is arranged at the top end of the liquid filling tube section 901, a liquid inlet 20 is arranged at the top end of the liquid inlet tube section 902, an injection channel 21 is formed below the liquid inlet 20, a sealing plate 22 is movably arranged in the inner cavity of the liquid inlet tube section 902, the injection tube 17 penetrates through the liquid inlet 20 to push the sealing plate 22 to move to open the injection channel 21, and after the injection tube 17 leaves the liquid inlet tube section 902, the sealing plate 22 returns to the original position to seal the injection channel 21.

In the invention, the shape of the reagent tube 9 is limited, the reagent tube comprises a liquid containing tube section 901 and a liquid inlet tube section 902, a sealing plate 22 is arranged in the liquid inlet tube section 902, the injection channel 21 is sealed by the sealing plate 22, and the injection channel 21 can be automatically opened by pushing the sealing plate 22 through the injection tube 17, so that the filling steps of the reagent are simplified, and meanwhile, when the reagent is extracted manually, the cover is not required to be opened manually, and the extraction efficiency of the reagent is improved.

The transmission component 1002 and the driving component 1202 may be a hydraulic cylinder transmission mechanism or a screw transmission mechanism, which are both in the prior art and are not described herein again;

in this embodiment, the feeding mechanism 10, the filling mechanism 11, and the discharging mechanism 12 operate synchronously to complete feeding, filling, and discharging simultaneously, wherein the reagent tube 9 is lifted by the lifting member 1001 of the feeding mechanism 10 until the reagent tube 9 is moved to the feeding area 4 by the driving member 1002, and the lifting member is separated from the reagent tube 9 to complete feeding; the lifting component 1101 of the filling mechanism 11 drives the filling component 1102 to descend, the injection tube 17 penetrates through the liquid inlet 20 to push the sealing plate 22 to move so as to open the injection channel 21, and the injection tube 17 injects the reagent into the reagent tube 9 and returns to the original position, so that filling is completed; the clamping part 1201 of the blanking mechanism 12 extracts the reagent tube 9 until the driving part 1202 drives the reagent tube 9 to move to the upper part of the blanking slideway 19, and the clamping part 1201 loosens the fixation of the reagent tube 9 to complete blanking.

Because the feeding mechanism 10 and the blanking mechanism 12 run synchronously, the invention specifically provides an embodiment, and one mechanism is utilized to drive the lifting part 1001 and the clamping part 1201 to move horizontally, so that the structure compactness is improved;

specifically, the mounting frame plate 23 is arranged on the workbench 1, the transmission part 1002 and the driving part 1202 are the same mechanism, the transmission part 1002 comprises a driving cylinder 24 and a fixed rod 25, the telescopic end of the driving cylinder 24 is provided with a transmission rod 26, the transmission rod 26 is in sliding sleeve joint with the fixed rod 25 outside the fixed rod 25, and the material lifting part 1001 and the clamping part 1201 are both fixedly mounted on the transmission rod 26;

the material lifting component 1001 and the clamping component 1201 both comprise a lifting cylinder 27 and a fixing block 28, two clamping plates 29 and two power cylinders 30 are installed at the bottom of the fixing block 28 in a sliding mode, and the power cylinders 30 drive the two clamping plates 29 to move relatively.

In this embodiment, the specifically defined transmission component 1002 and the drive component 1202 are the same structure, and the structure includes a drive cylinder 24 and a fixing rod 25, the outside of the fixing rod 25 is slidably sleeved with the transmission rod 26, the telescopic end of the drive cylinder 24 is connected with the transmission rod 26, when the device is operated, the drive cylinder 24 drives the transmission rod 26 to move horizontally, and the material lifting component 1001 and the clamping component 1201 realize synchronous movement; the material lifting component 1001 and the clamping component 1201 both comprise two clamping plates 29 and a power cylinder 30, the power cylinder 30 is used for pushing the clamping plates 29 to move relatively, clamping of the plurality of reagent tubes 9 is completed, and synchronous feeding and discharging of the plurality of reagent tubes 9 are guaranteed.

It should be noted that, a plurality of mounting holes adapted to the reagent tubes 9 are formed in the object placing plate 8, the reagent tubes 9 are inserted into the mounting holes, in order to ensure that the clamping plate 29 can contact with the reagent tubes 9 to clamp the reagent tubes 9, the depth of the mounting holes is smaller than the height of the reagent tubes 9, and the top ends of the reagent tubes 9 extend to the upper side of the mounting holes.

Further, rubber layers 31 are mounted on opposite sides of the clamping plate 29, and mounting grooves 32 matched with the reagent tubes 9 are formed in the rubber layers 31.

The setting of rubber layer 31 can protect reagent pipe 9, prevents that it is impaired at the extrusion in-process, and simultaneously, the stability to reagent pipe 9 centre gripping can be improved in seting up of mounting groove 32, prevents that reagent pipe 9 from dropping at the clamping-process.

Example 2

It should be noted that, in the present invention, the sealing plate 22 is movably mounted on the liquid inlet pipe section 902, and the movable mounting may be up and down movement or rotation, when the sealing plate 22 is at the initial position, the injection channel 21 is closed to prevent the reagent from flowing out of the pipe body, when the liquid needs to be filled or extracted, the injection channel 21 can be opened by pushing the sealing plate 22 through the liquid inlet 20 by the injection pipe 17, and after the injection pipe 17 leaves, the sealing plate 22 can be reset to close the injection channel 21 again.

The present invention provides an embodiment in which the seal plate 22 is able to rotate in the inlet pipe section 902:

referring to fig. 3, specifically, the inner cavity of the liquid inlet pipe section 902 is rotatably provided with a rotating rod 33, a sealing plate 22 is fixedly arranged on the rotating rod 33, and the diameter of the sealing plate 22 is matched with the inner diameter of the liquid inlet pipe section 902;

the sealing plate 22 comprises a gravity part 2201 and a light plate part 2202, the weight of the light plate part 2202 is lower than that of the gravity part 2201, and the injection tube 17 penetrates through the liquid inlet 20 to push the light plate part 2202 to rotate downwards and open the injection channel 21;

the inner wall of the liquid inlet pipe section 902 is provided with a baffle block 34, and the baffle block 34 is used for preventing the sealing plate 22 from rotating under the action of gravity

In this embodiment, since the sealing plate 22 includes the gravity portion 2201 and the light plate portion 2202, the gravity portion 2201 has a downward rotating force without an external force, and the light plate portion 2202 moves upward to block the sealing plate 22 from rotating by gravity, so as to seal the injection channel 21, and when it is necessary to inject or extract a reagent, the injection tube 17 passes through the liquid inlet 20 and applies a downward pressure to the light plate portion 2202, so that the light plate portion 2202 rotates downward, and the gravity portion 2201 rotates upward and moves above the liquid inlet section 902 through the liquid inlet 20, so as to finally open the injection channel 21, and when the injection tube 17 leaves, the sealing plate 22 rotates in the opposite direction under the action of gravity to reset, so as to close the injection channel 21 again.

Further, two baffle blocks 34 are provided, in which the bottom surface of one baffle block 34 abuts against the top surface of light plate portion 2202, and the top surface of the other baffle block 34 abuts against the bottom surface of gravity portion 2201.

Stop the closing plate 22 rotation under the action of gravity, only need a baffle piece 34 can, set up two baffle pieces 34 in this embodiment, be in order to stop closing plate 22 rotation in, improve sealed effect, because closing plate 22 contradicts with baffle piece 34 under the action of gravity, the two closely links to each other, seals injection channel 21 once more, prevents that reagent from flowing out.

Example 3

The present invention provides an embodiment in which the seal plate 22 can move up and down in the feed pipe section 902:

referring to fig. 4 and 5, specifically, the liquid inlet pipe section 902 is arranged in a truncated cone shape, two sealing plates 22 are oppositely arranged in an inner cavity of the liquid inlet pipe section 902, the top of each sealing plate 22 is fixedly connected with the top of the liquid inlet pipe section 902 through a return spring 35, and the outer sides of the sealing plates 22 are slidably connected with the inner wall of the liquid inlet pipe section 902;

the injection tube 17 passes through the inlet 20 to push the sealing plates 22 to slide down along the inner wall of the inlet pipe section 902, and the two sealing plates 22 are separated to open the injection channel 21.

In this embodiment, the sealing plate 22 can move along the inner wall of the liquid inlet pipe section 902 under the thrust action of the injection pipe 17, because the circular truncated cone-shaped arrangement of the liquid inlet pipe section 902, move down along with two sealing plates 22, gradually keep away from between the two, until the distance between the two is greater than the outer diameter of the injection pipe 17, the injection channel 21 is completely opened, and after the injection pipe 17 leaves the liquid inlet pipe section 902, the sealing plate 22 returns to the original position under the action of the reset spring 35, the injection channel 21 is sealed, the automatic opening and closing of the injection channel 21 are realized, and the convenience of reagent injection and extraction is improved.

Further, be equipped with hollow layer 36 between the inner wall of feed liquor pipeline section 902 and the outer wall, hollow layer 36's inner chamber is equipped with limiting plate 37, limiting plate 37 and hollow layer 36's inner wall laminating, the long type hole 38 with hollow layer 36 intercommunication is seted up to feed liquor pipeline section 902's inner wall, the inside in long type hole 38 is equipped with connecting rod 6, the both ends of connecting rod 6 are connected with limiting plate 37 and closing plate 22 respectively, and closing plate 22 seals the sealed hole.

In order to guarantee that the sealing plate 22 can move along the inner wall of the liquid inlet pipe section 902, in the embodiment, a hollow layer 36 is further arranged, a limiting plate 37 is arranged in the inner cavity of the hollow layer 36, meanwhile, a long hole 38 is formed in the inner wall of the liquid inlet pipe section 902, a connecting rod 6 is arranged inside the long hole 38, the limiting plate 37 and the sealing plate 22 are connected through the connecting rod 6, and due to the fact that the limiting plate 37 is attached to the inner wall of the hollow layer 36, the moving direction of the sealing plate 22 is limited, the sealing plate is prevented from descending along the vertical direction, and the injection channel 21 is ensured to be smoothly opened one by one.

Example 4

Aiming at the device for synthesizing and filling the trace oligonucleotide, the invention also provides a method for synthesizing and filling the trace oligonucleotide, which comprises the following steps:

s1, pouring the reagent tubes 9 into the vibrating disk 13, starting the vibrating disk 13, and discharging the reagent tubes 9 to the feeding track 14 in order;

s2, the feeding rail 14 drives the reagent tube 9 to move, and the feeding mechanism 10 extracts the materials of the feeding rail 14 to the feeding area 4 to finish feeding;

s3, the driving motor 3 and the feeding mechanism 10 run synchronously, the driving motor 3 drives the rotating platform 2 to rotate until the reagent tube 9 rotates to the filling area 5, meanwhile, the feeding mechanism 10 returns to the original position, and the material lifting part 1001 moves to the upper part of the feeding track 14;

s4, the feeding mechanism 10 and the filling mechanism 11 synchronously operate, the feeding mechanism 10 extracts the materials of the feeding track 14 to the feeding area 4, the injection pipe 17 of the filling mechanism 11 injects the reagent into the reagent pipe 9 and returns to the original position, and feeding and filling are completed simultaneously;

s5, the driving motor 3 and the feeding mechanism 10 continue to run synchronously, the driving motor 3 rotates until the filled reagent tube 9 moves to the blanking area 7, the filled reagent tube 9 moves to the filling area 5, and the feeding mechanism 10 returns to the original position, and the lifting member 1001 moves to the upper side of the feeding track 14;

s6, the feeding mechanism 10, the filling mechanism 11 and the blanking mechanism 12 synchronously run, and feeding, filling and blanking are completed simultaneously;

the lifting part 1001 of the feeding mechanism 10 lifts the reagent tube 9 until the transmission part 1002 drives the reagent tube 9 to move to the feeding area 4, and the lifting part is separated from the reagent tube 9 to complete feeding; the lifting component 1101 of the filling mechanism 11 drives the filling component 1102 to descend, the injection tube 17 penetrates through the liquid inlet 20 to push the sealing plate 22 to move so as to open the injection channel 21, and the injection tube 17 injects the reagent into the reagent tube 9 and returns to the original position, so that filling is completed; the clamping part 1201 of the blanking mechanism 12 extracts the reagent tube 9 until the driving part 1202 drives the reagent tube 9 to move to the upper part of the blanking slideway 19, and the clamping part 1201 loosens the fixation of the reagent tube 9 to complete blanking.

Further, in S6, when the feeding mechanism 10 and the discharging mechanism 12 are operated, the driving cylinder 24 is driven to operate the driving rod 26, and the driving rod 26 drives the lifting member 1001 and the holding member 1201 to move simultaneously.

The above is only a specific embodiment of the present invention, but the technical features of the present invention are not limited thereto. Any simple changes, equivalent substitutions or modifications made based on the present invention to solve the same technical problems and achieve the same technical effects are within the scope of the present invention.

Claims

1. The utility model provides a synthetic filling device of trace oligonucleotide, includes workstation (1), installs swivel stand (2) on workstation (1), and the power supply of swivel stand (2) is driving motor (3), its characterized in that: a feeding area (4), a filling area (5) and a discharging area (7) are arranged on the rotating table (2), object placing plates (8) are arranged on the feeding area (4), the filling area (5) and the discharging area (7), the object placing plates (8) are used for placing reagent tubes (9), a feeding mechanism (10) is arranged above the feeding area (4), a filling mechanism (11) is arranged above the filling area (5), and a discharging mechanism (12) is arranged above the discharging area (7);

the reagent tube feeding device is characterized in that a vibration disc (13) is further arranged on the workbench (1), a feeding track (14) is mounted outside the vibration disc (13), the vibration disc (13) sequentially discharges reagent tubes (9) onto the feeding track (14), the feeding track (14) is used for conveying materials, and the feeding mechanism (10) is used for extracting the materials of the feeding track (14) to the feeding area (4);

the feeding mechanism (10) comprises a lifting part (1001) and a transmission part (1002), the lifting part (1001) is used for extracting the reagent tube (9) and driving the reagent tube to move along the vertical direction, and the transmission part (1002) is used for driving the lifting part (1001) to move along the horizontal direction;

the filling mechanism (11) comprises a lifting component (1101), a filling component (1102) and a liquid medicine tank (15), the liquid medicine tank (15) is connected with the filling component (1102) through a conveying pipe (16), the lifting component (1101) is used for driving the filling component (1102) to ascend and descend, a plurality of injection pipes (17) are arranged on the filling component (1102), the injection pipes (17) are communicated with the conveying pipe (16), and the injection pipes (17) are used for injecting reagents into the reagent pipes (9);

a material receiving box (18) is further installed on the outer side of the workbench (1), a discharging slide way (19) is arranged between the material receiving box (18) and the rotating platform (2), the discharging mechanism (12) is used for extracting materials in the discharging area (7) to the discharging slide way (19), and the materials enter the material receiving box (18) along the discharging slide way (19);

the blanking mechanism (12) comprises a clamping part (1201) and a driving part (1202), the clamping part (1201) is used for clamping the reagent tube (9) and driving the reagent tube to move along the vertical direction, and the driving part (1202) is used for driving the clamping part (1201) to move along the horizontal direction;

reagent pipe (9) are including dress liquid pipe section (901), the top of dress liquid pipe section (901) is equipped with feed liquor pipe section (902), inlet (20) have been seted up at the top of feed liquor pipe section (902), injection channel (21) are formed to the below of inlet (20), the inner chamber movable mounting of feed liquor pipe section (902) has closing plate (22), injection tube (17) pass inlet (20) and promote closing plate (22) motion and open injection channel (21), and injection tube (17) leave behind feed liquor pipe section (902), closing plate (22) resume normal position and seal injection channel (21).

2. The loading device for micro-oligonucleotide synthesis according to claim 1, wherein: the workbench (1) is provided with a mounting frame plate (23), the transmission part (1002) and the driving part (1202) are the same mechanism, the transmission part (1002) comprises a driving cylinder (24) and a fixed rod (25), the telescopic end of the driving cylinder (24) is provided with a transmission rod (26), the transmission rod (26) is in sliding sleeve joint with the fixed rod (25) outside the fixed rod (25), and the material lifting part (1001) and the clamping part (1201) are fixedly mounted on the transmission rod (26);

the material lifting component (1001) and the clamping component (1201) both comprise a lifting cylinder (27) and a fixing block (28), two clamping plates (29) and two power cylinders (30) are installed at the bottom of the fixing block (28) in a sliding mode, and the power cylinders (30) drive the two clamping plates (29) to move relatively.

3. The loading device for micro-oligonucleotide synthesis according to claim 2, wherein: rubber layer (31) are all installed to the opposite side of grip block (29), offer on rubber layer (31) with reagent pipe (9) the mounting groove (32) of looks adaptation.

4. The loading device for micro-oligonucleotide synthesis according to claim 1, wherein: the inner cavity of the liquid inlet pipe section (902) is rotatably provided with a rotating rod (33), a sealing plate (22) is fixedly arranged on the rotating rod (33), and the diameter of the sealing plate (22) is matched with the inner diameter of the liquid inlet pipe section (902);

the sealing plate (22) comprises a gravity part (2201) and a light plate part (2202), the weight of the light plate part (2202) is lower than that of the gravity part (2201), and the injection tube (17) penetrates through the liquid inlet (20) to push the light plate part (2202) to rotate downwards and open the injection channel (21);

the inner wall of the liquid inlet pipe section (902) is provided with a baffle block (34), and the baffle block (34) is used for preventing the sealing plate (22) from rotating under the action of gravity.

5. The loading device for micro-oligonucleotide synthesis according to claim 1, wherein: the number of the baffle plates (34) is two, wherein the bottom surface of one baffle plate (34) is abutted against the top surface of the light plate part (2202), and the top surface of the other baffle plate (34) is abutted against the bottom surface of the gravity part (2201).

6. The loading device for micro-oligonucleotide synthesis according to claim 1, wherein: the liquid inlet pipe section (902) is arranged in a circular truncated cone shape, two sealing plates (22) are oppositely arranged in the inner cavity of the liquid inlet pipe section (902), the tops of the sealing plates (22) are fixedly connected with the top of the liquid inlet pipe section (902) through a return spring (35), and the outer sides of the sealing plates (22) are slidably connected with the inner wall of the liquid inlet pipe section (902);

the injection pipe (17) penetrates through the liquid inlet (20) to push the sealing plates (22) to slide downwards along the inner wall of the liquid inlet pipe section (902), and the two sealing plates (22) are separated to open the injection channel (21).

7. The loading device for micro-oligonucleotide synthesis according to claim 6, wherein: be equipped with hollow layer (36) between the inner wall of feed liquor pipeline section (902) and the outer wall, the inner chamber of hollow layer (36) is equipped with limiting plate (37), limiting plate (37) and the inner wall laminating of hollow layer (36), long type hole (38) with hollow layer (36) intercommunication are seted up to the inner wall of feed liquor pipeline section (902), the inside in long type hole (38) is equipped with connecting rod (6), the both ends of connecting rod (6) are connected with limiting plate (37) and closing plate (22) respectively, and closing plate (22) seal the sealed hole.

8. A filling method for synthesizing a micro-amount oligonucleotide according to claim 2, comprising the steps of:

s1, pouring the reagent tubes (9) into the vibrating disk (13), starting the vibrating disk (13), and discharging the reagent tubes (9) to the feeding track (14) in order;

s2, the feeding rail (14) drives the reagent tube (9) to move, and the feeding mechanism (10) extracts the materials of the feeding rail (14) to the feeding area (4) to finish feeding;

s3, the driving motor (3) and the feeding mechanism (10) run synchronously, the driving motor (3) drives the rotating platform (2) to rotate until the reagent tube (9) rotates to the filling area (5), the feeding mechanism (10) returns to the original position, and the lifting part (1001) moves to the position above the feeding track (14);

s4, the feeding mechanism (10) and the filling mechanism (11) synchronously operate, the feeding mechanism (10) extracts the materials of the feeding track (14) to the feeding area (4), the injection pipe (17) of the filling mechanism (11) injects the reagent into the reagent pipe (9) and returns to the original position, and feeding and filling are completed simultaneously;

s5, the driving motor (3) and the feeding mechanism (10) continue to synchronously operate, the driving motor (3) rotates until the filled reagent tube (9) moves to the blanking area (7), the filled reagent tube (9) moves to the filling area (5), meanwhile, the feeding mechanism (10) returns to the original position, and the lifting part (1001) moves to the position above the feeding track (14);

s6, the feeding mechanism (10), the filling mechanism (11) and the discharging mechanism (12) synchronously operate, and feeding, filling and discharging are completed simultaneously;

the reagent tube (9) is lifted by a lifting part (1001) of the feeding mechanism (10) until the transmission part (1002) drives the reagent tube (9) to move to the feeding area (4), and the lifting part is separated from the reagent tube (9) to finish feeding; the filling mechanism (11) is provided with a lifting component (1101) which drives a filling component (1102) to descend, the injection tube (17) penetrates through the liquid inlet (20) to push the sealing plate (22) to move so as to open the injection channel (21), and the injection tube (17) injects a reagent into the reagent tube (9) and restores the original position to complete filling; the clamping part (1201) of the blanking mechanism (12) extracts the reagent tube (9) until the driving part (1202) drives the reagent tube (9) to move to the upper part of the blanking slideway (19), and the clamping part (1201) loosens the fixation of the reagent tube (9) to complete blanking.

9. The loading device for micro-oligonucleotide synthesis according to claim 8, wherein: in S6, when the feeding mechanism (10) and the discharging mechanism (12) operate, the driving cylinder (24) drives the driving rod (26) to operate, and the driving rod (26) drives the lifting part (1001) and the clamping part (1201) to move simultaneously.