CN110713912A - Microorganism regular sampling detection device - Google Patents

Microorganism regular sampling detection device Download PDF

Info

Publication number
CN110713912A
CN110713912A CN201911089554.2A CN201911089554A CN110713912A CN 110713912 A CN110713912 A CN 110713912A CN 201911089554 A CN201911089554 A CN 201911089554A CN 110713912 A CN110713912 A CN 110713912A
Authority
CN
China
Prior art keywords
cavity
sliding
sampling
rack
block
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
CN201911089554.2A
Other languages
Chinese (zh)
Inventor
乐续春
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Pan'an Rouman Microbial Technology Co Ltd
Original Assignee
Pan'an Rouman Microbial Technology Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Pan'an Rouman Microbial Technology Co Ltd filed Critical Pan'an Rouman Microbial Technology Co Ltd
Priority to CN201911089554.2A priority Critical patent/CN110713912A/en
Publication of CN110713912A publication Critical patent/CN110713912A/en
Withdrawn legal-status Critical Current

Links

Images

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M33/00Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus
    • C12M33/04Means for introduction, transport, positioning, extraction, harvesting, peeling or sampling of biological material in or from the apparatus by injection or suction, e.g. using pipettes, syringes, needles
    • AHUMAN NECESSITIES
    • A61MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61LMETHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L2/00Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
    • A61L2/02Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using physical phenomena
    • A61L2/08Radiation
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12MAPPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00Means for regulation, monitoring, measurement or control, e.g. flow regulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

The invention relates to the field of microorganism culture, in particular to a microorganism periodic sampling detection device, which comprises a machine body and a sampling cavity arranged in the machine body, wherein a culture cavity communicated through an isolation channel is arranged in the bottom wall of the sampling cavity, a sliding cavity with a leftward opening is arranged in the end wall of the right side of the sampling cavity, a sliding frame with the left side extending into the sampling cavity is slidably arranged in the sliding cavity, and an absorption device for absorbing strains is fixedly arranged in the sliding frame in the sampling cavity. The observation by a microscope is convenient for follow-up observation, and the degree of automation is high and more reliable.

Description

Microorganism regular sampling detection device
Technical Field
The invention relates to the field of microorganism culture, in particular to a microorganism regular sampling detection device.
Background
In the process of microorganism culture, the cultured sanitary ware needs to be sampled and detected regularly, in the sampling process, the mixed bacteria are mixed due to the irregular operation, the growth environment of microorganism strains is influenced, the traditional sampling method has low automation degree, the on-time sampling is forgotten, and the data is lost, so that a microorganism regular sampling and detecting device is needed to be arranged to solve the problems.
Disclosure of Invention
The invention aims to provide a microorganism regular sampling detection device, which can overcome the defects in the prior art, thereby improving the practicability of equipment.
The technical scheme adopted by the invention for solving the technical problems is as follows: the invention relates to a microorganism regular sampling detection device, which comprises a machine body and a sampling cavity arranged in the machine body, wherein a culture cavity communicated through an isolated channel is arranged in the bottom wall of the sampling cavity, a sliding cavity with a left opening is arranged in the end wall on the right side of the sampling cavity, a sliding frame with the left side extending into the sampling cavity is arranged in the sliding cavity in a sliding way, a suction device for sucking strains is fixedly arranged in the sliding frame in the sampling cavity, a lifting device capable of driving the sliding frame to move up and down is arranged in the sliding cavity, a conveying cavity communicated with the sliding frame is arranged in the end wall on the left side of the sampling cavity, a sliding block is arranged in the conveying cavity in a sliding way, a conveying device for conveying glass sheets is arranged in the sliding block, the conveying device can convey the glass sheets placed in a storage cavity with a forward opening arranged in the, the fixed dog that sets up of glass piece front side opening department, when hoisting device drives the carriage moves down, the response piece moves down along with the carriage, when the inductor senses the carriage for the first time, the inductor control the sterilamp that sets up in the intercommunication chamber between sample chamber and the storing chamber is right the operation of disinfecting in sample chamber and the storing chamber, after the completion of disinfecting, the carriage continues to move down under the hoisting device effect, the carriage moves down the in-process the hoisting device drives the isolated device that sets up in the isolated passageway end wall will sample the chamber with sample chamber with cultivate the chamber intercommunication, at this moment the sampling tube that sets up in the suction means inserts in cultivateing the intracavity, when the carriage moves down to the sliding chamber bottom, the carriage triggers the isolated device to close, at the moment, the sampling tube is inserted into the isolation channel, the isolation device triggers the suction device to suck strains in the culture cavity, the lifting device turns to drive the sliding frame to move upwards, after the sampling tube is moved out of the isolation channel, an isolation plate arranged in the isolation device moves rightwards under the action of a first spring arranged on the left side of the isolation plate to seal the isolation channel, so that the sampling cavity and the culture cavity are isolated, when the sliding frame moves upwards to drive the sensing block to move upwards to abut against the sensor, the sensor controls the conveying device to convey a glass sheet into the sampling cavity, when the glass sheet is positioned at the bottom of the sampling tube, the sliding frame continues to move upwards under the action of the lifting device, and when a first sliding rod arranged in the sampling tube abuts against the top wall of the sampling cavity, the strains sucked by the suction device are extruded onto the glass sheet, at the moment, the conveying device drives the strain to move to the lower side of the storage cavity, at the moment, the glass sheet in the storage cavity moves downwards to cover the upper side of the strain, and at the moment, a sealing door which is hinged and connected in a swinging cavity arranged in the front side end wall of the machine body is opened, so that the upper glass piece and the lower glass piece of the strain are pushed out of the device, and the strain sampling is completed.
Furthermore, the suction device comprises an extraction cavity arranged in the sampling tube, a suction tube communicated with the outside is arranged in the bottom wall of the extraction cavity, a push block is arranged in the extraction cavity in a sliding manner, a first sliding rod fixedly connected with the push block is arranged in a first sliding hole arranged in the top wall of the extraction cavity in a sliding manner, a second spring is arranged between the first sliding rod and the top wall of the sampling tube, a first guide sliding groove communicated with the outside is arranged in the left end wall of the extraction cavity, a first rack with the tail end inserted into a first clamping groove arranged in the left end wall of the push block is arranged in the first guide sliding groove in a sliding manner, a second guide sliding groove with a leftward opening is arranged in the left end wall of the sampling tube, a second rack is arranged in the second guide sliding groove in a sliding manner, and a third spring is arranged between the second rack and the right end wall of the second guide sliding groove, the second guide chute and the first guide chute are communicated with each other to form a first gear cavity, a first gear which is meshed with the second rack and the first rack is slidably arranged in the first gear cavity, the isolation plate moves rightwards to extrude the second rack, and therefore the first rack is driven to move out of the first clamping groove, and the push block is unlocked.
Further, the lifting device comprises a first threaded hole which is arranged in the sliding frame and is vertically communicated, a first threaded rod is connected with the first threaded hole in an internal thread mode, the top end of the first threaded rod is in power connection with a first motor fixedly arranged in the top wall of the sliding cavity, a pushing cavity is arranged in the bottom wall of the sliding cavity, a first rotating shaft which is rotatably arranged in the top wall of the pushing cavity and is provided with a cam at the bottom end is fixedly arranged, a ratchet mechanism with one-way transmissibility is arranged between the first rotating shaft and the first threaded rod, the first threaded rod positively rotates to drive the sliding frame to move downwards, the first threaded rod drives the cam to rotate through the ratchet mechanism, and when the sliding frame moves upwards, the first threaded rod reversely rotates to drive the first rotating shaft to rotate.
Further, conveyor include the second screw hole that link up about setting up in the sliding block, second screw hole female connection have with carry the second threaded rod that second motor power that sets up in the chamber left side end wall is connected, be provided with the ascending recess of opening in the sliding block roof, slidable is provided with the push pedal in the recess, the push pedal with only one between the sliding block up end the height of glass piece, be provided with the ascending second sliding hole of opening in the recess diapire, slidable in the second sliding hole be provided with push pedal fixed connection's second slide bar, the push pedal with be provided with the fourth spring between the recess diapire, be provided with the third sliding hole to the left in the sliding block left side end wall, slidable is provided with the third slide bar in the third sliding hole, the third slide bar with be provided with between the third sliding hole roof drive after the third slide bar displacement drive And the third sliding rod is reset.
Further, the isolation device comprises a sliding groove which is arranged in the end wall of the isolation channel and is communicated with the pushing cavity, the isolating plate is arranged in the sliding groove in a sliding manner, a communicating hole which is communicated up and down is arranged in the isolating plate, a third guide chute with a downward opening is arranged in the top wall of the sliding chute, a third rack matched with a second clamping groove arranged in the top wall of the isolation plate is arranged in the third guide chute in a sliding manner, a fifth spring is arranged between the third rack and the top wall of the sliding groove, a fourth sliding guide groove with an upward opening is arranged in the bottom wall of the sampling cavity, a fourth rack is arranged in the fourth guide chute in a sliding manner, a second gear cavity is communicated and arranged between the fourth guide chute and the third guide chute, and a second gear which is meshed with the third rack and the fourth rack is slidably arranged in the second gear cavity.
Further, be provided with the intercommunication in the side end wall behind the transport chamber the second slide opening and the U-shaped groove in swing chamber, U-shaped inslot slidable is provided with the U-shaped piece, U-shaped piece right side bottom with sealing door butt, U-shaped piece right side top with the glass piece butt, the U-shaped piece with be provided with the sixth spring between the U-shaped groove, be provided with the outside filler hole of intercommunication in the wall of cultivation chamber right side end, be provided with the sealed piece of detachable in the filler hole.
The invention has the beneficial effects that: the regular sampling detection device for the microorganisms, provided by the invention, can realize regular sampling detection of a culture solution of microorganism strains, the device is used for isolating the culture chamber from the sampling chamber and sterilizing the interior of the sampling chamber before sampling, the probability of mixing of mixed bacteria in the culture sample in the device is effectively reduced, the device can be used for automatically placing the sampled culture solution between two sterilized glass sheets, the subsequent observation by using a microscope is facilitated, the automation degree is high, and the device is more reliable.
Drawings
In order to more clearly illustrate the embodiments of the invention or the technical solutions in the prior art, the drawings used in the description of the embodiments or the prior art will be briefly described below, and it is obvious that the drawings in the following description are only some embodiments of the invention, and it is obvious for those skilled in the art that other drawings can be obtained based on these drawings without creative efforts.
The invention is further illustrated with reference to the following figures and examples.
FIG. 1 is a schematic view of the overall structure of a device for sampling and detecting microorganisms periodically according to the present invention.
Fig. 2 is a schematic view of the structure a-a in fig. 1.
Fig. 3 is an enlarged schematic view of B in fig. 1.
Fig. 4 is an enlarged schematic view of C in fig. 1.
Detailed Description
The invention will now be described in detail with reference to fig. 1-4, wherein for ease of description the orientations described hereinafter are now defined as follows: the up, down, left, right, and front-back directions described below correspond to the up, down, left, right, and front-back directions in the projection relationship of fig. 1 itself.
The microorganism regular sampling detection device described with reference to fig. 1-4 comprises a body 10 and a sampling cavity 11 disposed in the body 10, wherein a culture cavity 32 communicated with the bottom wall of the sampling cavity 11 through an isolation channel 30 is disposed in the bottom wall of the sampling cavity 11, a sliding cavity 21 with a leftward opening is disposed in the end wall of the right side of the sampling cavity 11, a sliding frame 19 with a left side extending into the sampling cavity 11 is slidably disposed in the sliding cavity 21, a suction device 99 for sucking strains is fixedly disposed in the sliding frame 19 in the sampling cavity 11, a lifting device 98 capable of driving the sliding frame 19 to move up and down is disposed in the sliding cavity 21, a conveying cavity 33 communicated with the sliding frame is disposed in the end wall of the left side of the sampling cavity 11, a sliding block 40 is slidably disposed in the conveying cavity 33, and a conveying device 97 for conveying glass sheets is disposed in, conveyor 97 can be with glass piece 47 placed in the storing chamber 44 that the opening that sets up in the roof of transport chamber 33 was forward is sent into sample chamber 11 one by one, the fixed dog 75 that sets up of glass piece 47 front side opening department, when hoisting device 98 drove carriage 19 moved down, response piece 18 accompanies carriage 19 moves down, when inductor 20 sensed for the first time carriage 19, inductor 20 control sample chamber 11 with the sterilamp 46 that sets up in the intercommunication chamber 45 between the storing chamber 44 is right sample chamber 11 and carry out sterilization operation in the storing chamber 44, treat to disinfect the completion after, carriage 19 continues to move down under hoisting device 98 acts on, carriage 19 moves down the in-process hoisting device 98 drives isolation device 96 that sets up in the isolated passageway 30 end wall will sample chamber 11 with cultivate the chamber 32 intercommunication, at this time, the sampling tube 15 arranged in the suction device 99 is inserted into the culture cavity 32, when the sliding frame 19 moves downwards to the bottom of the sliding cavity 21, the sliding frame 19 triggers the isolation device 96 to close, at this time, because the sampling tube 15 is inserted into the isolation channel 30, the isolation device 96 triggers the suction device 99 to suck strains in the culture cavity 32, at this time, the lifting device 98 turns to drive the sliding frame 19 to move upwards, after the sampling tube 15 moves out of the isolation channel 30, the isolation plate 29 arranged in the isolation device 96 moves rightwards under the action of the first spring 31 arranged at the left side of the isolation plate to close the isolation channel 30, so as to isolate the sampling cavity 11 from the culture cavity 32, when the sliding frame 19 moves upwards to drive the sensing block 18 to move upwards to abut against the sensor 20, the sensor 20 controls the conveying device 97 to convey a glass sheet into the sampling cavity 11, when the glass block is positioned at the bottom of the sampling tube 15, the sliding frame 19 continues to move upwards under the action of the lifting device 98, when the first sliding rod 12 arranged in the sampling tube 15 is abutted against the top wall of the sampling cavity 11, strains sucked by the sucking device 99 are extruded onto the glass sheet, at the moment, the conveying device 97 drives the strains to move leftwards to the lower part of the storage cavity 44, at the moment, the glass sheet 47 in the storage cavity 44 moves downwards to cover the upper part of the strains, at the moment, the sealing door 54 hinged and connected in the swing cavity 53 arranged in the front side end wall of the machine body 10 is opened, so that the upper glass and the lower glass of the strains are pushed out of the device, and therefore, the sampling of the strains is.
Advantageously, the suction device 99 comprises a drawing cavity 74 arranged in the sampling tube 15, a drawing tube 23 communicated with the outside is arranged in the bottom wall of the drawing cavity 74, a push block 73 is slidably arranged in the drawing cavity 74, the first sliding rod 12 fixedly connected with the push block 73 is slidably arranged in a first sliding hole 14 arranged in the top wall of the drawing cavity 74, a second spring 13 is arranged between the first sliding rod 12 and the top wall of the sampling tube 15, a first guide chute 71 communicated with the outside is arranged in the left end wall of the drawing cavity 74, a first rack 70 with the end inserted into a first clamping groove 72 arranged in the left end wall of the push block 73 is slidably arranged in the first guide chute 71, a second guide chute 65 with a leftward opening is arranged in the left end wall of the sampling tube 15, and a second rack 66 is slidably arranged in the second guide chute 65, a third spring 67 is arranged between the second rack 66 and the right side end wall of the second slide guiding groove 65, a first gear cavity 69 is arranged between the second slide guiding groove 65 and the first slide guiding groove 71 in a communicating manner, a first gear 68 which is engaged with the second rack 66 and the first rack 70 is slidably arranged in the first gear cavity 69, the isolation plate 29 is extruded by moving right to move the second rack 66, so that the first rack 70 is driven to move out of the first clamping groove 72, and the push block 73 is unlocked.
Advantageously, said lifting means 98 comprise a first threaded hole 17, which passes through the carriage 19 from top to bottom, a first threaded rod 22 is connected with the first threaded hole 17 through internal threads, the tail end of the top of the first threaded rod 22 is in power connection with a first motor 16 fixedly arranged in the top wall of the sliding cavity 21, a pushing cavity 26 is arranged in the bottom wall of the sliding cavity 21, a first rotating shaft 76 with a bottom end fixedly provided with a cam 25 is rotatably arranged in the top wall of the pushing cavity 26, a ratchet mechanism 24 with one-way transmissibility is arranged between the first rotating shaft 76 and the first threaded rod 22, when the sliding frame 19 is driven to move downwards by the forward rotation of the first threaded rod 22, the first threaded rod 22 drives the cam 25 to rotate through the ratchet mechanism 24, when the first threaded rod 22 rotates reversely to drive the sliding frame 19 to move upwards, the first threaded rod 22 cannot drive the first rotating shaft 76 to rotate.
Advantageously, the conveying device 97 comprises a second threaded hole 38 penetrating left and right in the sliding block 40, a second threaded rod 34 connected with the second motor 39 arranged in the left end wall of the conveying cavity 33 in a power mode is connected in the second threaded hole 38 in a threaded mode, a groove 43 with an upward opening is arranged in the top wall of the sliding block 40, a push plate 48 is arranged in the groove 43 in a sliding mode, the height of only one glass sheet 47 is between the push plate 48 and the upper end surface of the sliding block 40, a second sliding hole 41 with an upward opening is arranged in the bottom wall of the groove 43, a second sliding rod 42 fixedly connected with the push plate 48 is arranged in the second sliding hole 41 in a sliding mode, a fourth spring 49 is arranged between the push plate 48 and the bottom wall of the groove 43, a third sliding hole 36 with a leftward opening is arranged in the left end wall of the sliding block 40, and a third sliding rod 35 is arranged in the third sliding hole, a reset device 37 is disposed between the third sliding rod 35 and the top wall of the third sliding hole 36, and drives the third sliding rod 35 to reset after the third sliding rod 35 displaces.
Beneficially, the isolation device 96 includes a sliding groove 58 disposed in an end wall of the isolation channel 30 and communicated with the pushing cavity 26, the isolation plate 29 is slidably disposed in the sliding groove 58, a communicating hole 59 penetrating up and down is disposed in the isolation plate 29, a third guide sliding groove 56 with a downward opening is disposed in a top wall of the sliding groove 58, a third rack 57 matched with a second clamping groove 60 disposed in a top wall of the isolation plate 29 is slidably disposed in the third guide sliding groove 56, a fifth spring 55 is disposed between the third rack 57 and the top wall of the sliding groove 58, a fourth guide sliding groove 61 with an upward opening is disposed in a bottom wall of the sampling cavity 11, a fourth rack 64 is slidably disposed in the fourth guide sliding groove 61, a second gear cavity 63 is disposed between the fourth guide sliding groove 61 and the third guide sliding groove 56, and a second gear cavity 63 engaged with the third rack 57 and the fourth rack 64 is slidably disposed in the second gear cavity 63 And a gear 62.
Beneficially, a U-shaped groove 51 communicating the second sliding hole 41 and the swinging cavity 53 is arranged in the rear side end wall of the conveying cavity 33, a U-shaped block 52 is slidably arranged in the U-shaped groove 51, the bottom of the right side of the U-shaped block 52 abuts against the sealing door 54, the top of the right side of the U-shaped block 52 abuts against the glass sheet 47, a sixth spring 50 is arranged between the U-shaped block 52 and the U-shaped groove 51, a filling hole 28 communicating with the outside is arranged in the end wall of the right side of the culture cavity 32, and a detachable sealing block 27 is arranged in the filling hole 28.
The fixing and connecting method in this embodiment includes, but is not limited to, bolting, welding, and the like.
As shown in fig. 1-4, in the initial state of the apparatus of the present invention, the return means 37 is in the tension state and the second spring 13 is in the compression state.
Sequence of mechanical actions of the whole device:
when the device works, the sealing door 54 is rotated forwards, the storage cavity 44 is communicated with the outside at the moment, the glass sheet 47 is placed into the storage cavity 44, the sealing door 54 is closed, then the sealing block 27 is pulled out of the filling hole 28, microbial strain culture solution is filled into the culture cavity 32 through the filling hole 28, the sealing block 27 is placed into the filling hole 28 again, when the growth state of the microbial strain is required to be detected, the first motor 16 is started to drive the first threaded rod 22 to rotate forwards, so that the sliding frame 19 is driven to move downwards, so that the sampling tube 15 is driven to move downwards, when the sensor 20 senses the sensing block 18 for the first time, the sterilizing lamp 46 is started to sterilize the storage cavity 44 and the sampling cavity 11, and after the sterilization is finished, the sliding frame 19 continues to move downwards, thereby driving the sampling tube 15 to move downwards, the first threaded rod 22 drives the cam 25 to rotate when rotating, thereby driving the isolation plate 29 to move left, when the isolation plate 29 moves left to drive the communication hole 59 to align with the isolation channel 30, the second slot 60 aligns with the third guide slot 56, at this time, the third rack 57 is inserted into the second slot 60, at this time, the sampling tube 15 moves downwards continuously to penetrate through the isolation channel 30 to extend into the culture solution in the culture chamber 32, when the sliding rack 19 moves downwards to abut against the fourth rack 64, the second rack 66 just enters into the communication hole 59, at this time, the sliding rack 19 moves downwards to press against the fourth rack 64, the fourth rack 64 moves downwards to drive the third rack 57 to move upwards to move out of the second slot 60, at this time, the cam 25 device is arranged at the rightmost side, at this time, the isolation plate 29 moves to the right under the action of the first spring 31, at this time, the end wall of the communication hole 59 presses the second rack 66 to the right, the second rack 66 moves to the right to drive the first rack 70 to move out of the first slot 72 to the left, at this time, the push block 73 moves up under the action of the first sliding rod 12, so that the culture solution in the culture chamber 32 is drawn into the drawing chamber 74 through the drawing tube 23, at this time, the first motor 16 is started to drive the first threaded rod 22 to rotate in the reverse direction, so that the carriage 19 moves up, the carriage 19 moves up to drive the sampling tube 15 to move out of the isolation channel 30, at this time, the isolation plate 29 moves to the right under the action of the first spring 31 to seal the isolation channel 30, and when the carriage 19 moves up to drive the sensing block 18 to abut against the sensor 20, the sensor 20 controls the second motor 39 to start to drive the second threaded rod 34 to rotate, thereby driving the slide block 40 to move to the right, thereby driving one glass sheet 47 to move to the right, after the slide block 40 moves to the right, the third slide rod 35 moves to the left, at this time, the second slide rod 42 moves down to drive the push plate 48 to move down, thereby driving the glass sheet 47 to move down, when the glass sheet 47 moves to the position right below the sampling tube 15, the slide frame 19 continues to move up, thereby driving the first slide rod 12 to abut against the top wall of the sampling cavity 11, at this time, the first slide rod 12 moves down to drive the push block 73 to move down to extrude the culture solution in the extraction cavity 74 to the position above the glass sheet 47 through the dip tube 23, at this time, the second motor 39 rotates reversely to drive the glass sheet 47 to move to the left, when the U third slide rod 35 abuts against the left end wall of the conveying cavity 33, the third slide rod 35 moves to the right to move the second slide rod 42 upward, when the groove 43 is aligned with the storage cavity 44, the glass sheet 47 in the storage cavity 44 slides to the position above the glass sheet 47 in the second sliding rod 42 to seal the culture solution, at the moment, the sealing door 54 is opened, the U-shaped block 52 moves forwards under the action of the sixth spring 50, so that the upper glass sheet 47 and the lower glass sheet 47 of the culture solution are ejected out of the device, at the moment, a microscope is used for penetrating through the growth condition of strains in the culture solution between the two glass sheets 47, the steps are periodically repeated, and the growth state of microorganisms in the culture cavity 32 can be penetrated through.
The above embodiments are merely illustrative of the technical ideas and features of the present invention, and the purpose thereof is to enable those skilled in the art to understand the contents of the present invention and implement the present invention, and not to limit the protection scope of the present invention. All equivalent changes and modifications made according to the spirit of the present invention should be covered within the protection scope of the present invention.

Claims (6)

1. The utility model provides a microorganism periodic sampling detection device, includes the fuselage and set up in sample chamber in the fuselage, its characterized in that: the bottom wall of the sampling cavity is internally provided with a culture cavity communicated through an isolation channel, the right side end wall of the sampling cavity is internally provided with a sliding cavity with a left opening, the sliding cavity is internally slidably provided with a sliding frame with a left side extending into the sampling cavity, the sliding frame in the sampling cavity is internally and fixedly provided with a suction device for sucking strains, the sliding cavity is internally provided with a lifting device capable of driving the sliding frame to move up and down, the left side end wall of the sampling cavity is internally provided with a conveying cavity communicated with the sampling cavity, the conveying cavity is internally and slidably provided with a sliding block, the sliding block is internally provided with a conveying device for conveying glass sheets, the conveying device can convey the glass sheets placed in a storage cavity with a forward opening, which is arranged in the top wall of the conveying cavity, into the sampling cavity one by one, a stop block is fixedly arranged, the induction block moves down along with the sliding frame, when the inductor senses the sliding frame for the first time, the inductor controls a sterilizing lamp arranged in a communication cavity between the sampling cavity and the storage cavity to sterilize the sampling cavity and the storage cavity, after sterilization is completed, the sliding frame continues to move down under the action of the lifting device, the lifting device drives an isolation device arranged in the end wall of the isolation channel to communicate the sampling cavity with the sampling cavity and the culture cavity in the sliding frame moving down process, a sampling tube arranged in the suction device is inserted into the culture cavity at the moment, when the sliding frame moves down to the bottom of the sliding cavity, the sliding frame triggers the isolation device to close, at the moment, because the sampling tube is inserted into the isolation channel, the isolation device triggers the suction device to suck strains in the culture cavity, at the moment, the lifting device turns to drive the sliding frame to move upwards, after the sampling tube is moved out of the isolation channel, an isolation plate arranged in the isolation device moves rightwards under the action of a first spring arranged on the left side of the isolation plate to close the isolation channel, so that the sampling cavity is isolated from the culture cavity, when the sliding frame moves upwards to drive the induction block to move upwards to abut against the inductor, the inductor controls the conveying device to convey a glass sheet into the sampling cavity, when the glass sheet is positioned at the bottom of the sampling tube, the sliding frame continues to move upwards under the action of the lifting device, when a first sliding rod arranged in the sampling tube abuts against the top wall of the sampling cavity, strains sucked by the suction device are extruded onto the glass sheet, at the moment, the conveying device drives the strains to move leftwards to the lower part of the storage cavity, at the moment, the glass sheet in the storage cavity moves downwards to cover the strains, and opening a sealing door which is hinged and connected in a swinging cavity arranged in the front side end wall of the machine body, so that the upper glass piece and the lower glass piece of the strain are pushed out of the device, and the strain sampling is finished.
2. The periodic sampling and testing device for microorganisms according to claim 1, wherein: the suction device comprises an extraction cavity arranged in the sampling tube, a dip tube communicated with the outside is arranged in the bottom wall of the extraction cavity, a push block is arranged in the extraction cavity in a sliding manner, a first sliding rod fixedly connected with the push block is arranged in a first sliding hole arranged in the top wall of the extraction cavity in a sliding manner, a second spring is arranged between the first sliding rod and the top wall of the sampling tube, a first guide chute communicated with the outside is arranged in the left end wall of the extraction cavity, a first rack with the tail end inserted into a first clamping groove arranged in the left end wall of the push block is arranged in the first guide chute in a sliding manner, a second guide chute with a leftward opening is arranged in the left end wall of the sampling tube, a second rack is arranged in the second guide chute in a sliding manner, and a third spring is arranged between the second rack and the right end wall of the second guide chute, the second guide chute and the first guide chute are communicated with each other to form a first gear cavity, a first gear which is meshed with the second rack and the first rack is slidably arranged in the first gear cavity, the isolation plate moves rightwards to extrude the second rack, and therefore the first rack is driven to move out of the first clamping groove, and the push block is unlocked.
3. The periodic sampling and testing device for microorganisms according to claim 1, wherein: lifting device includes the first screw hole that link up from top to bottom that sets up in the carriage, first screw hole female connection has first threaded rod, first threaded rod top end with the first motor power of the internal fixation setting of sliding chamber roof is connected, be provided with the top in the sliding chamber diapire and push away the chamber, push away the rotatable first pivot that is provided with the fixed cam that is provided with in chamber roof bottom, first pivot with be provided with the ratchet that has one-way transmissibility between the first threaded rod, first threaded rod forward rotates and drives when the carriage moves down, first threaded rod passes through ratchet drives the cam rotates, first threaded rod antiport drives when the carriage shifts up, first threaded rod can't drive first pivot is rotated.
4. The periodic sampling and testing device for microorganisms according to claim 1, wherein: conveyor includes the second screw hole that link up about setting up in the sliding block, second screw hole female connection have with carry the second threaded rod that second motor power that sets up in the chamber left side end wall is connected, be provided with the ascending recess of opening in the sliding block roof, slidable is provided with the push pedal in the recess, the push pedal with only one between the sliding block up end the height of glass piece, be provided with the ascending second sliding hole of opening in the recess diapire, slidable is provided with in the second sliding hole with push pedal fixed connection's second slide bar, the push pedal with be provided with the fourth spring between the recess diapire, be provided with the third sliding hole of opening left in the sliding block left side end wall, slidable is provided with the third slide bar in the third sliding hole, the third slide bar with be provided with between the third sliding hole roof drive after the third slide bar displacement the third slide bar Reset device for resetting.
5. The periodic sampling and testing device for microorganisms according to claim 1, wherein: the isolation device comprises a sliding groove which is arranged in the end wall of the isolation channel and communicated with the pushing cavity, the isolating plate is arranged in the sliding groove in a sliding manner, a communicating hole which is communicated up and down is arranged in the isolating plate, a third guide chute with a downward opening is arranged in the top wall of the sliding chute, a third rack matched with a second clamping groove arranged in the top wall of the isolation plate is arranged in the third guide chute in a sliding manner, a fifth spring is arranged between the third rack and the top wall of the sliding groove, a fourth sliding guide groove with an upward opening is arranged in the bottom wall of the sampling cavity, a fourth rack is arranged in the fourth guide chute in a sliding manner, a second gear cavity is communicated and arranged between the fourth guide chute and the third guide chute, and a second gear which is meshed with the third rack and the fourth rack is slidably arranged in the second gear cavity.
6. The periodic sampling and testing device for microorganisms according to claim 4, wherein: the conveying cavity rear side end wall is internally provided with a communicated second sliding hole and a U-shaped groove of the swinging cavity, the U-shaped groove is internally provided with a U-shaped block in a slidable manner, the bottom of the right side of the U-shaped block is abutted against a sealing door, the top of the right side of the U-shaped block is abutted against a glass sheet, a sixth spring is arranged between the U-shaped block and the U-shaped groove, a filling hole communicated with the outside is arranged in the end wall of the right side of the cultivation cavity, and a detachable sealing block is arranged in the filling hole.
CN201911089554.2A 2019-11-08 2019-11-08 Microorganism regular sampling detection device Withdrawn CN110713912A (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN201911089554.2A CN110713912A (en) 2019-11-08 2019-11-08 Microorganism regular sampling detection device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN201911089554.2A CN110713912A (en) 2019-11-08 2019-11-08 Microorganism regular sampling detection device

Publications (1)

Publication Number Publication Date
CN110713912A true CN110713912A (en) 2020-01-21

Family

ID=69214956

Family Applications (1)

Application Number Title Priority Date Filing Date
CN201911089554.2A Withdrawn CN110713912A (en) 2019-11-08 2019-11-08 Microorganism regular sampling detection device

Country Status (1)

Country Link
CN (1) CN110713912A (en)

Cited By (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111632179A (en) * 2020-05-25 2020-09-08 上海履济技术服务中心 Sterilization verifier and application thereof
CN112481074A (en) * 2020-11-18 2021-03-12 中国人民解放军陆军军医大学第二附属医院 Intestinal canal bacterium liquid culture apparatus
CN113136314A (en) * 2021-05-14 2021-07-20 唐山师范学院 Microbial cultivation integration equipment
CN115177752A (en) * 2022-09-13 2022-10-14 江苏中慧元通生物科技股份有限公司 Sterilization apparatus of culture medium for vaccine production

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN111632179A (en) * 2020-05-25 2020-09-08 上海履济技术服务中心 Sterilization verifier and application thereof
CN112481074A (en) * 2020-11-18 2021-03-12 中国人民解放军陆军军医大学第二附属医院 Intestinal canal bacterium liquid culture apparatus
CN113136314A (en) * 2021-05-14 2021-07-20 唐山师范学院 Microbial cultivation integration equipment
CN113136314B (en) * 2021-05-14 2022-02-25 唐山师范学院 Microbial cultivation integration equipment
CN115177752A (en) * 2022-09-13 2022-10-14 江苏中慧元通生物科技股份有限公司 Sterilization apparatus of culture medium for vaccine production

Similar Documents

Publication Publication Date Title
CN110713912A (en) Microorganism regular sampling detection device
CN105620994B (en) A kind of intelligence sample is filed for reference cabinet system
WO2017148392A1 (en) Vacuum blood collection tube labelling machine and vacuum blood collection tube conveying and labelling method
CN208689063U (en) A kind of sample analysis system
WO2022143456A1 (en) Nucleic acid extraction and fluorescent pcr detection system
CN209866123U (en) Test tube placing box for physical examination center
CN112592814A (en) Microorganism periodic sampling detection device and sampling method
CN110354283B (en) Intelligent dinner plate disinfection cabinet convenient to take
CN112623369A (en) Bagging equipment with open bag lock material structure
CN109486666B (en) Microbial gene quantitative sampling device for biological control
CN110352757B (en) Apple bagging machine
CN108698005B (en) Liquid supply system
CN212829615U (en) Culture medium box transfer mechanism and microbial sample intelligent processing system
CN111529732A (en) Medical test tube internal standard sterilizing machine and using method thereof
CN110118672B (en) Material sampling device of belt conveyor
JP2002104586A (en) Lifter for filling constant quantity of liquid into container
CN106476463A (en) A kind of processing method of document handling apparatus
CN112938873A (en) Production line is carried with packing to food processing
CN213229534U (en) Storage box for preventive vaccine test tube
CN206142408U (en) Sample holder pushing hands mechanism with switch and be written into direction
CN220625838U (en) Rice sampler
CN218908555U (en) Automatic feeding cabinet for chemical analysis
CN214004633U (en) A inoculation device for cherry tomato embryo fermentation
CN219771053U (en) Automatic turn-over of gluing box closes equipment of box
CN117778149B (en) Yeast fermentation product detection sample liquid extraction device

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
WW01 Invention patent application withdrawn after publication
WW01 Invention patent application withdrawn after publication

Application publication date: 20200121