CN116019858A - Compound preparation containing bacillus bailii, preparation method and application thereof - Google Patents
Compound preparation containing bacillus bailii, preparation method and application thereof Download PDFInfo
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- CN116019858A CN116019858A CN202211259679.7A CN202211259679A CN116019858A CN 116019858 A CN116019858 A CN 116019858A CN 202211259679 A CN202211259679 A CN 202211259679A CN 116019858 A CN116019858 A CN 116019858A
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- enterococcus faecalis
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- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention provides a compound preparation containing bacillus belicus, a preparation method and application thereof, and relates to the technical field of biological preparations. The invention provides a compound preparation containing bacillus belicus, which comprises bacillus belicus, bacillus subtilis, enterococcus faecalis and Chinese medicinal concentrated solution; the mass ratio of bacillus bailii, bacillus subtilis, enterococcus faecalis to the traditional Chinese medicine concentrated solution is 3-5:4-6:3-5:2-4. Experiments prove that the bacillus belicus-containing compound preparation can effectively prevent and treat the poultry callus on sole and the occurrence of related bad symptoms such as leg diseases caused by mycotoxin, feed and trace element deficiency, protect intestinal tracts, reduce the morbidity of the callus on meat ducks, broilers and geese, further reduce feed conversion ratio and death rate, improve the final weight and provide a development way for non-antibiotic cultivation.
Description
Technical Field
The invention relates to the technical field of biological preparations, in particular to a compound preparation containing bacillus bailii, a preparation method and application thereof.
Background
In poultry, dermatitis of Foot Pads (FPD) is a problem that seriously affects poultry health, productivity, animal welfare, etc. FPD can cause necrotic lesions on the plantar surface of poultry, a skin inflammation common in intensive farming, also known as hoof dermatitis and contact dermatitis, which occur on the heels and toes of poultry. Under suitable development times and conditions, this situation can develop within 7 d.
The main problem of FPD is the increase in water content of the cushioning material, which is initially that the plantar skin begins to change color, and then slowly ulcers, which can cause swelling, heating and redness under the skin, causing thickening of the skin surface. When dermatitis is severe, bacteria (e.g., escherichia coli, staphylococcus aureus, etc.) may grow in large amounts at the ulcer, causing pain to the poultry, especially leg diseases and various behavioral disorders, resulting in serious economic loss.
Poultry callus occurs for a variety of reasons, including feeding environmental conditions such as litter material, litter moisture, litter thickness, litter pH, etc., drinkers, seasonal factors, diet nutrient deficiency (biotin, zinc), cereal raw materials, diet protein levels and sources, ion balance, mold, mycotoxin, gender and weight, breed, etc. Therefore, in order to avoid the occurrence of the callus on the sole, proper padding, proper drinking water device, good feeding management (temperature and humidity, checking ions in water quality) and nutrition technology (providing a complete ration formula, preventing the deficiency of nutrients of broilers and adding complex enzymes into ration) need to be provided on the padding selection. In view of the influence of the poultry callus on the production performance, it is imperative to find a simple and feasible control method with good effect and low cost.
Disclosure of Invention
Therefore, the invention aims to provide the compound preparation containing bacillus bailii, which can prevent and treat poultry callus, improve poultry breeding productivity and reduce death elimination rate.
In order to solve the technical problems, the invention provides the following technical scheme:
the invention provides a compound preparation containing bacillus belicus, which comprises bacillus belicus, bacillus subtilis, enterococcus faecalis and Chinese medicinal concentrated solution; the mass ratio of bacillus bailii, bacillus subtilis, enterococcus faecalis to the traditional Chinese medicine concentrated solution is 3-5:4-6:3-5:2-4.
Preferably, the bacillus belicus is deposited with the accession number of CICC 25046; the deposit number of the bacillus subtilis is CICC6060; the enterococcus faecalis has a deposit number of CICC 21869.
Preferably, the traditional Chinese medicine concentrated solution comprises the following raw materials in parts by weight: 50-80 parts of radix scutellariae, 260-350 parts of pulsatilla chinensis, 450-550 parts of hairyvein agrimony and 400-550 parts of poria cocos.
Preferably, the traditional Chinese medicine concentrated solution is prepared by distilling and extracting baical skullcap root, chinese pulsatilla root, hairyvein agrimony and Indian buead, concentrating and mixing with auxiliary materials.
More preferably, the auxiliary material is any one of starch, maltodextrin and glucose.
The invention also provides a preparation method of the compound preparation, which comprises the following steps:
mixing bacillus behenii powder, bacillus subtilis powder, enterococcus faecalis powder and the traditional Chinese medicine concentrated solution to obtain the compound preparation containing bacillus behenii.
Preferably, the bacillus beliensis bacterial powder is obtained by microencapsulating bacterial mud obtained by fermenting bacillus beliensis and centrifuging.
Preferably, the bacillus subtilis bacterial powder is obtained by fermenting bacillus subtilis, centrifuging and microencapsulating; the enterococcus faecalis bacterial powder is obtained by fermenting enterococcus faecalis, centrifuging and coating in a microencapsulated way.
Preferably, the microencapsulation coating is a soluble starch.
The invention also provides the application of the compound preparation or the preparation method in poultry cultivation.
The invention has the beneficial effects that:
the invention provides a compound preparation containing bacillus belicus, which combines a microecological preparation and a traditional Chinese veterinary medicine preparation, wherein bacillus belicus, bacillus subtilis and enterococcus faecalis can reduce the occurrence of leg diseases of poultry. The baikal skullcap root added in the compound preparation has the functions of clearing heat and drying dampness, purging fire and removing toxin, clearing heat and nourishing yin, eliminating dampness, nourishing blood and regulating yin and yang balance of the organism, and can stop bleeding; the pulsatilla chinensis is cold in nature and is commonly used for clearing heat and detoxicating, cooling blood and stopping bleeding, promoting liver toxin expelling and the like; the hairyvein agrimony has the functions of astringing to stop bleeding, tonifying deficiency and inhibiting bacteria and expelling parasites after entering heart, liver and spleen channels; poria has effects of inducing diuresis to alleviate edema, invigorating spleen, relieving diarrhea, nourishing heart, and tranquilizing; the Chinese medicine can protect poultry viscera, improve immunity of poultry and detoxication and antitoxic capability of organism, increase survival rate, feed conversion rate and improve production performance. Experiments prove that the compound preparation can effectively prevent and treat the poultry callus and the related adverse symptoms such as leg diseases caused by mycotoxin, feed and trace element deficiency, reduce the incidence of the callus on meat ducks, broiler chickens and meat geese, further reduce feed conversion ratio and death rate, improve the final weight and provide a development path for nonresistant cultivation.
Biological preservation description:
the bacillus belicus of the invention is named in a classification way: bacillus beliae is preserved in China center for type culture Collection of industrial microorganisms, with the preservation number of CICC 25046, the preservation date of 2020, 12 months and 24 days, and the preservation address: building No. 3 of 24 th yard No. 6 in Jiuxian bridge, the region of Chaoyang in Beijing city.
The bacillus subtilis provided by the invention is classified and named: bacillus subtilis purchased from China center for type culture collection of Industrial microorganisms, accession number CICC6060, accession number: building No. 3 of 24 th yard No. 6 in Jiuxian bridge, the region of Chaoyang in Beijing city.
The enterococcus faecalis is classified and named: enterococcus faecalis purchased from China industry microbiological culture Collection center with accession number CICC 21869, accession number: building No. 3 of 24 th yard No. 6 in Jiuxian bridge, the region of Chaoyang in Beijing city.
Detailed Description
The invention provides a compound preparation containing bacillus belicus, which comprises bacillus belicus, bacillus subtilis, enterococcus faecalis and Chinese medicinal concentrated solution; the mass ratio of bacillus bailii, bacillus subtilis, enterococcus faecalis to the traditional Chinese medicine concentrated solution is 3-5:4-6:3-5:2-4.
In the invention, the bacillus beleiensis is preferably a strain which is autonomously separated and stored by research and development departments of Dalian three-instrument animal medicine limited company, and the storage number of the bacillus beleiensis is CICC 25046; the bacillus subtilis is preferably purchased from China center for type culture collection (CICC) 6060; the enterococcus faecalis is preferably purchased from China center for type culture collection of Industrial microorganisms, and the preservation number of the enterococcus faecalis is CICC 21869. In the present invention, the total number of colonies of Bacillus belicus is preferably (20-30). Times.10 10 CFU/g, more preferably (22-28). Times.10 10 CFU/g; the total number of colonies of the enterococcus faecalis is preferably (15-25). Times.10 10 CFU/g, more preferably 21X 10 10 CFU/g; the total number of colonies of the Bacillus subtilis is preferably (20-30). Times.10 10 CFU/g, more preferably23-28)×10 10 CFU/g。
In the invention, the traditional Chinese medicine concentrated solution preferably comprises the following raw materials in parts by weight: 50-80 parts of radix scutellariae, 260-350 parts of pulsatilla chinensis, 450-550 parts of hairyvein agrimony and 400-550 parts of poria cocos; the traditional Chinese medicine concentrated solution more preferably comprises the following raw materials in parts by weight: 55-70 parts of radix scutellariae, 280-300 parts of pulsatilla chinensis, 460-500 parts of hairyvein agrimony and 440-500 parts of poria cocos. In the invention, the traditional Chinese medicine concentrated solution is preferably prepared by distilling and extracting baical skullcap root, chinese pulsatilla root, hairyvein agrimony and Indian buead, concentrating and then mixing with auxiliary materials; in the distillation extraction, the feed liquid ratio of the traditional Chinese medicine raw materials to water is preferably 1:8-12, more preferably 1:10; the number of distillations is preferably 3; the concentration is preferably performed by concentrating the extract in a volume ratio of 1:5. In the invention, the auxiliary material is preferably any one of starch, maltodextrin or glucose. The invention utilizes the concentrated traditional Chinese medicine liquid to be mixed with auxiliary materials, and can ensure that the effective components of the traditional Chinese medicine concentrated liquid are protected. In the invention, the traditional Chinese medicine concentrated solution is preferably powder or liquid preparation; the traditional Chinese medicine concentrated solution powder is preferably obtained by distilling and extracting radix scutellariae, chinese pulsatilla root, hairyvein agrimony and poria cocos, concentrating, mixing with auxiliary materials and drying; the drying mode is not particularly limited, and a conventional drying mode in the art may be adopted.
The invention also provides a preparation method of the compound preparation, which comprises the following steps:
mixing bacillus behenii powder, bacillus subtilis powder, enterococcus faecalis powder and the traditional Chinese medicine concentrated solution to obtain the compound preparation containing bacillus behenii. In the invention, the bacillus belicus bacterial powder is obtained by microencapsulating and coating bacterial mud obtained by fermenting bacillus belicus and centrifuging; the bacillus subtilis bacterial powder is obtained by fermenting bacillus subtilis, centrifuging and microencapsulating; the enterococcus faecalis bacterial powder is obtained by fermenting enterococcus faecalis, centrifuging and coating in a microencapsulated way. In the present invention, the microencapsulation coating is preferably spherical microencapsulation coating using soluble starch. The fermentation modes of the bacillus belicus, the bacillus subtilis and the enterococcus faecalis are not particularly limited, and the bacillus belicus, the bacillus subtilis and the enterococcus faecalis can be obtained by adopting a conventional strain fermentation method in the field. The method for coating the micro-capsules is not particularly limited, and the strain is coated by adopting a conventional micro-capsule coating method in the field. In the present invention, the mixing is preferably three-dimensional mixing; the three-dimensional mixing is an all-dimensional mixing. The three-dimensional mixing is preferably carried out by adopting a three-dimensional mixer, so that the uniformity of the compound preparation is ensured.
The present invention will be described in detail below with reference to examples for the purpose of making the objects, technical solutions and advantages of the present invention more apparent, but they should not be construed as limiting the scope of the present invention.
In the following examples, conventional methods are used unless otherwise specified.
Materials, reagents and the like used in the examples described below are commercially available unless otherwise specified.
Example 1
1) Respectively fermenting bacillus belicus, enterococcus faecalis and bacillus subtilis according to respective conventional fermentation processes, microencapsulating and coating the obtained bacterial sludge by using soluble starch, and drying to obtain bacterial powder of each strain; wherein, the total number of bacterial colonies of each bacterial powder is: bacillus bailii 25×10 10 CFU/g; enterococcus faecalis 15X 10 10 CFU/g; bacillus subtilis is 20×10 10 CFU/g；
2) Weighing 55 parts of baical skullcap root, 260 parts of Chinese pulsatilla root, 500 parts of hairyvein agrimony and 400 parts of Indian buead, distilling and extracting the weighed Chinese medicinal materials and water according to the feed liquid ratio of 1:10, concentrating the extracting solution according to the ratio of 1:5 after 3 times of extraction, and drying by using auxiliary material starch to obtain Chinese medicinal powder;
3) The bacillus behenensis bacterial powder, the enterococcus faecalis bacterial powder, the bacillus subtilis bacterial powder and the traditional Chinese medicine powder are effectively mixed in a three-dimensional mode according to the mass ratio of 3:4:3:4, and are packaged for standby.
Example 2
Bacillus behenii, enterococcus faecalis and bacillus subtilis are respectively treated according to the following stepsFermenting respectively by conventional fermentation process, microencapsulating and coating the obtained bacterial sludge by using soluble starch, and drying to obtain bacterial powder of each strain respectively; wherein, the total number of bacterial colonies of each bacterial powder is: bacillus bailii 30×10 10 CFU/g; enterococcus faecalis 21X 10 10 CFU/g; the bacillus subtilis is 30 multiplied by 10 10 CFU/g；
2) Weighing 70 parts of baical skullcap root, 300 parts of Chinese pulsatilla root, 550 parts of hairyvein agrimony and 500 parts of Indian buead, distilling and extracting the weighed Chinese medicinal materials and water according to a feed liquid ratio of 1:10, concentrating the extracting solution according to a ratio of 1:5 after 3 times of extraction, and drying by using auxiliary materials glucose to obtain Chinese medicinal powder:
3) The bacillus behenensis bacterial powder, the enterococcus faecalis bacterial powder, the bacillus subtilis bacterial powder and the traditional Chinese medicine powder are effectively mixed in a three-dimensional mode according to the mass ratio of 5:4:4:5, and are packaged for standby.
Example 3
Respectively fermenting bacillus belicus, enterococcus faecalis and bacillus subtilis according to respective conventional fermentation processes, microencapsulating and coating the obtained bacterial sludge by using soluble starch, and drying to obtain bacterial powder of each strain; wherein, the total number of bacterial colonies of each bacterial powder is: bacillus bailii 20×10 10 CFU/g; enterococcus faecalis 15X 10 10 CFU/g; the bacillus subtilis is 25 multiplied by 10 10 CFU/g；
2) Weighing 80 parts of baical skullcap root, 280 parts of Chinese pulsatilla root, 460 parts of hairyvein agrimony and 440 parts of Indian buead, distilling and extracting the weighed Chinese medicinal materials and water according to the feed liquid ratio of 1:10, concentrating the extracting solution according to the ratio of 1:5 after 3 times of extraction, and drying by using auxiliary material maltodextrin to obtain Chinese medicinal powder;
3) The bacillus behenensis bacterial powder, the enterococcus faecalis bacterial powder, the bacillus subtilis bacterial powder and the traditional Chinese medicine powder are effectively mixed in a three-dimensional mode according to the mass ratio of 4:4:3:4, and are packaged for standby.
Comparative example 1
The procedure for the preparation of the compound formulation was the same as in example 1, except that: bacillus belicus was not added to this comparative example.
Comparative example 2
The procedure for the preparation of the compound formulation was the same as in example 1, except that: enterococcus faecalis was not added in this comparative example.
Comparative example 3
The procedure for the preparation of the compound formulation was the same as in example 1, except that: in this comparative example, no Bacillus subtilis was added.
Comparative example 4
The procedure for the preparation of the compound formulation was the same as in example 1, except that: in this comparative example, scutellariae radix and radix Pulsatillae were removed.
Example 4
Taking broiler chickens as experimental objects, selecting five houses for each object, wherein each 4500 broiler chickens is provided with a group of control groups: normal drinking and feeding; two test groups: the broiler chickens in the period from 1 to 21 days of age are respectively fed with 200g/T feed of the compound preparation of the embodiment 1 to 3 of the invention, and the broiler chickens in the period from 21 days of age to out-of-stock are respectively fed with 400g/T of the compound preparation of the embodiment 1 of the invention; two comparative examples were set simultaneously: 200g/T of the compound preparation of the comparative examples 1-4 are respectively fed. During the test, the mental state of the test animal was observed at any time, the occurrence of the callus on sole inflammation was recorded at any time, and the production performance-related index was calculated after the end of the test, to obtain tables 1 to 7. The calculation modes of the incidence rate and the death rate of the broiler foot pad inflammation are as follows:
incidence of footpad inflammation = number of episodes (only)/total number of trials (only) ×100%;
mortality = number of dead (only)/total number of trials (only) ×100%.
1. Examples 1-3 and comparative examples 1-4 broiler test results:
table 1 example 1 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence rate, feed-meat ratio and death rate of the callus on the sole of the experimental group are extremely lower than those of the control group (p < 0.01), and the final weight is extremely higher than that of the control group (p < 0.01), so that the preparation has an effective prevention effect on the broiler chicken cultivation.
Table 2 example 2 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
As can be seen from the test results, the incidence of callus inflammation, feed-to-meat ratio, and death rate of the group of example 2 were significantly lower than that of the control group (p < 0.01), and the final weight was significantly higher than that of the control group (p < 0.01). Although the component content was changed, the effect of example 2 was superior to that of example 1.
Table 3 example 3 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the test results, it can be seen that the incidence of callus inflammation, feed-to-meat ratio, and death rate of example 3 group were significantly lower than that of the control group (p < 0.01), and the final weight was significantly higher than that of the control group (p < 0.01).
Table 4 comparative example 1 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the test results, it can be seen that each group of comparative example 1 had a higher incidence of callus inflammation, feed-to-meat ratio, and death rate than the group of example 1, and that the end weight of the group of comparative example 1 was extremely lower than that of the group of example 1 (p < 0.01). Thus, bacillus belicus plays an important role in the preparation, and the incidence rate, feed-meat ratio, last and death rate of the callus inflammation have extremely remarkable influence.
Table 5 comparative example 2 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
As can be seen from the test results, the incidence rate of the foot pad inflammation of each group of the comparative example 2 is extremely higher than that of the group of the example 1 (p < 0.01), the feed-meat ratio and the death rate are extremely higher than that of the group of the example 1 (p < 0.01), and no obvious difference exists in the final weight. The result shows that the effect of enterococcus faecalis on various indexes of the examined broiler chickens is obviously lower than that of bacillus bailii.
Table 6 comparative example 3 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
As can be seen from the test results, the incidence of foot-pad inflammation, feed-to-meat ratio, and death rate of each group of comparative example 3 were all significantly higher than that of the group of example 1 (p < 0.01), and the final weight of the group of comparative example was significantly lower than that of the group of example 1 (p < 0.01). As can be seen from the results, the addition of bacillus subtilis in the preparation has a great influence on various indexes of broiler chickens, so that the strain is indispensable as a composite preparation.
Table 7 comparative example 4 broiler test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
As can be seen from the test results, the incidence of callus on the sole, feed/meat ratio, and death rate of each group of comparative example 4 were all significantly higher than those of the group of example 1 (p < 0.01), and the final weight of the group of comparative example was significantly lower than that of the group of example 1 (p < 0.01). The result shows that the addition of no baikal skullcap root and pulsatilla root in the preparation has great influence on various indexes of the broiler chickens.
In conclusion, bacillus belicus, enterococcus faecalis, bacillus subtilis and traditional Chinese medicine components in the composite preparation act synergistically with each other, so that the composite preparation has the effect of preventing and treating poultry callus.
2. Examples 1-3 and comparative examples 1-4 meat duck test results:
to further illustrate the effect of the compound formulation of the present invention in other poultry, the same experimental group and procedure of the broiler chickens of this example were used to develop relevant tests in the clinic of meat ducks and meat geese, and the results are shown in tables 8-21.
Table 8 example 1 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
It can be seen that the control group (p < 0.01) had significantly lower foot inflammation, feed conversion and death rate, and the control group (p < 0.05) had significantly higher final weight.
Table 9 example 2 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
It can be seen that the incidence of callus on the sole of example 2 group was significantly lower than that of the control group (p < 0.01), the feed conversion ratio and the death rate were significantly lower than those of the control group (p < 0.05), and the final weight was significantly higher than that of the control group (p < 0.05).
Table 10 example 3 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
It can be seen that the incidence of the callus inflammation and the feed conversion ratio of the meat ducks in the example 3 group are extremely higher than those in the test group (p < 0.01), the final weight is extremely higher than that in the control group (p < 0.05), and the death rate is extremely lower than that in the control group (p < 0.05).
Table 11 comparative example 1 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the test results, the incidence rate, feed-to-meat ratio and death rate of the callus on the sole of comparative example 1 were extremely higher than those of the group of example 1 (p < 0.01), and the final weight was extremely lower than those of the group of example 1 (p < 0.01). The bacillus belicus plays a key role in the compound preparation.
Table 12 comparative example 2 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the test results, the incidence of the foot pad inflammation, the feed-to-meat ratio and the death rate of comparative example 2 were significantly higher than those of example 1 group (p < 0.01), and the final weight was significantly lower than that of example 1 group (p < 0.01). Indicating that enterococcus faecalis plays an important role in the use of the compound preparation.
Table 13 comparative example 3 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the test results, the incidence of the foot pad inflammation, the feed-to-meat ratio and the death rate of comparative example 3 were extremely significantly higher than those of example 1 group (p < 0.01), and the final weight was extremely lower than that of example 1 group (p < 0.01). The bacillus subtilis plays a probiotic role in the compound preparation.
Table 14 comparative example 4 meat duck test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the test results, it can be seen that the incidence of callus inflammation, feed-to-meat ratio and death rate of comparative example 4 were significantly higher than those of example 1 group (p < 0.01), and the final weight was significantly lower than that of example 1 group (p < 0.01). The radix scutellariae and the pulsatillae are proved to play a probiotic role in the use of the compound preparation.
3. Examples 1-3 and comparative examples 1-4 meat goose test results:
table 15 example 1 meat goose test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence rate, feed-meat ratio and death rate of the callus on the foot of the experimental group in the embodiment 1 are extremely lower than those of the control group (p < 0.01), and the final weight is extremely higher than that of the control group (p < 0.01), so that the preparation has an effective prevention effect on the callus on the foot in the meat goose culture.
Table 15 example 2 results of meat goose test
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence of callus inflammation and the death rate of the experimental group in the example 2 are extremely lower than that of the control group (p < 0.01), the difference of feed-to-meat ratio is not obvious (p > 0.01), and the final weight is extremely higher than that of the control group (p < 0.01).
Table 17 example 3 meat goose test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence of callus inflammation and the death rate of the experimental group in the example 3 are extremely lower than that of the control group (p < 0.01), the difference of feed-to-meat ratio is not obvious (p > 0.01), and the final weight is extremely higher than that of the control group (p < 0.01).
Table 18 comparative example 1 meat goose test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
As can be seen from the experimental results, the incidence of foot-pad inflammation, the rate of death and panning of the experimental group of example 1 were significantly lower than that of the groups of comparative example 1 (p < 0.01), the feed-to-meat ratio was significantly lower than that of the groups of comparative example (p < 0.05), and the final weight was significantly higher than that of the control group (p < 0.01).
Table 19 comparative example 2 meat goose test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence of callus inflammation, the rate of death and the rate of pulp panning were significantly lower in the experimental group of example 1 than in the group of comparative example 2 (p < 0.05), the feed-to-meat ratio was significantly lower in the group of comparative example (p < 0.01), and the last significant higher in the control group (p < 0.05).
Table 20 comparative example 3 meat goose test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence of callus inflammation, the rate of death and the rate of pulp panning were significantly lower in the experimental group of example 1 than in the group of comparative example 3 (p < 0.01), the feed conversion was significantly lower in the group of comparative example (p < 0.05), and the last significant higher in the control group (p < 0.05).
Table 21 comparative example 4 meat goose test results
Note that: the same letters in table 1 indicate that the differences are not significant, different letters indicate that the differences are significant, and the large letters indicate that the differences are extremely significant.
From the experimental results, the incidence of callus inflammation, the rate of death and the rate of pulp panning were significantly lower in the experimental group of example 1 than in the group of comparative example 4 (p < 0.01), the feed conversion was significantly lower in the group of comparative example (p < 0.05), and the last significant higher in the control group (p < 0.05).
In conclusion, the bacillus bailii compound preparation can reduce the incidence rate of the callus on sole, and can improve the nutrition absorption capacity and the disease resistance of poultry, thereby improving the production performance.
Example 5
After the foot pad inflammation of the broiler chickens occurs in clinic, the sick chickens are picked up for separate feeding, and the treatment mode is to feed 400g/T of the compound preparation of the invention 2 according to the age of 1-21 days, and feed 800g/T of the compound preparation of the invention 2 after 21 days. The treatment was carried out in which stage, i.e., at which stage dose, and the effect of the treatment was observed, and the results are shown in tables 22 and 23.
Table 22 results of 10 day-old broiler test
The table shows that the preparation has a cure effect on 10-day-old disease test chickens, and the average cure rate is 77.5% on the basis of the test.
Table 23 test results of 28 day old broilers
The table shows that the preparation has a cure effect on 10-day-old disease test chickens, and the average cure rate is 47.5% on the basis of the test.
The compound preparation can effectively prevent and treat the occurrence of the related adverse symptoms such as the poultry callus and leg diseases caused by mycotoxin, feed and trace element deficiency, and the preparation can reduce the incidence of the poultry callus by examining the feed conversion ratio, the final weight and the death rate of test animals, thereby reducing the feed conversion ratio and the death rate and improving the final weight.
The foregoing description is only illustrative of the present invention and is not intended to limit the scope of the invention, and all equivalent structures or equivalent processes or direct or indirect application in other related arts are included in the scope of the present invention.
Claims (10)
1. The compound preparation containing bacillus belicus is characterized by comprising bacillus belicus, bacillus subtilis, enterococcus faecalis and traditional Chinese medicine concentrated solution; the mass ratio of bacillus bailii, bacillus subtilis, enterococcus faecalis to the traditional Chinese medicine concentrated solution is 3-5:4-6:3-5:2-4.
2. The compound preparation according to claim 1, wherein the bacillus besseyi has a deposit number of cic 25046; the deposit number of the bacillus subtilis is CICC6060; the enterococcus faecalis has a deposit number of CICC 21869.
3. The compound preparation according to claim 1, wherein the traditional Chinese medicine concentrated solution comprises the following raw materials in parts by weight: 50-80 parts of radix scutellariae, 260-350 parts of pulsatilla chinensis, 450-550 parts of hairyvein agrimony and 400-550 parts of poria cocos.
4. The compound preparation according to claim 3, wherein the concentrated Chinese medicine liquid is prepared by extracting radix Scutellariae, radix Pulsatillae, herba et Gemma Agrimoniae and Poria by distillation, concentrating, and mixing with adjuvants.
5. The compound preparation according to claim 4, wherein the auxiliary material is any one of starch, maltodextrin or glucose.
6. A method for preparing the compound preparation of claim 1, which is characterized by comprising the following steps:
mixing bacillus behenii powder, bacillus subtilis powder, enterococcus faecalis powder and the traditional Chinese medicine concentrated solution to obtain the compound preparation containing bacillus behenii.
7. The preparation method of claim 6, wherein the bacillus beliensis powder is obtained by microencapsulating bacterial sludge obtained by fermenting bacillus beliensis and centrifuging.
8. The preparation method of claim 6, wherein the bacillus subtilis powder is obtained by fermenting bacillus subtilis, centrifuging and microencapsulating; the enterococcus faecalis bacterial powder is obtained by fermenting enterococcus faecalis, centrifuging and coating in a microencapsulated way.
9. The method of claim 7 or 8, wherein the microencapsulation coating is a soluble starch.
10. Use of a compound formulation according to any one of claims 1 to 5 or a method of preparation according to any one of claims 6 to 9 in poultry farming.
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| WO2014151837A1 (en) * | 2013-03-15 | 2014-09-25 | Bayer Cropscience Lp | A bacillus subtilis strain for use in maintaining or improving the paw health of poultry |
| CN115044505A (en) * | 2022-06-09 | 2022-09-13 | 江南大学 | Antibacterial lipopeptide produced by bacillus belgii and application of antibacterial lipopeptide in cosmetics and foods |
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| CN117106640A (en) * | 2023-08-08 | 2023-11-24 | 大连三仪动物药品有限公司 | Biological agent for efficiently degrading mycotoxin and preparation method thereof |
| CN117106640B (en) * | 2023-08-08 | 2024-02-02 | 大连三仪动物药品有限公司 | Biological agent for efficiently degrading mycotoxin and preparation method thereof |
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