CN116019731A - Herba centellae compound with safety acne removing effect for prepubertal period - Google Patents
Herba centellae compound with safety acne removing effect for prepubertal period Download PDFInfo
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- CN116019731A CN116019731A CN202211428790.4A CN202211428790A CN116019731A CN 116019731 A CN116019731 A CN 116019731A CN 202211428790 A CN202211428790 A CN 202211428790A CN 116019731 A CN116019731 A CN 116019731A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The invention discloses a centella asiatica compound with a safe acne removing effect aiming at prepubertal stage, which mainly comprises the following components: madecassoside, a wound healing villus and octanoylglycine; the hair-wound treating flower comprises hair-wound treating flower extract and auxiliary materials, wherein the auxiliary materials comprise glycerol, water, potassium sorbate, sodium citrate, citric acid and sodium metabisulfite. The centella asiatica compound disclosed by the invention comprises the following components in parts by weight: 5-30 parts of madecassoside, 25-60 parts of a wound-healing erigeron breviscapus, 10-45 parts of octanoylglycine, 8-25 parts of a PH regulator and the balance of water. According to the invention, the madecassoside and the wound-healing villus flower are combined with the octanoylglycine to be compounded and applied to the raw material formula of the nursing product for preventing skin acne, thereby having multiple effects of resisting bacteria, resisting inflammation, relieving and repairing and balancing grease.
Description
Technical Field
The invention relates to the technical field of skin care products, in particular to a centella asiatica compound with a safety acne removing effect before puberty.
Background
The intrinsic cause of acne in prepubertal skin is mainly due to the massive secretion of sebum, abnormal keratinization of the pilo-sebaceous duct orifice, propionibacterium acnes colonization, immune inflammatory response and genetic correlation; the external factors are mainly caused by factors such as emotion, diet, improper use of skin care products, sun drying and the like. The symptoms are mainly acne, the early stage related to microorganisms is mainly caused by streptococcus and the middle and later stage is mainly caused by propionibacterium acnes, and the treatment scheme can use anti-acne skin care products with keratolytic or exfoliating functions, anti-inflammatory and antibacterial anti-acne skin care products and other treatment means through external and oral antibacterial drugs.
Madecassoside (MC) is the highest content pentacyclic triterpene compound of centella asiatica, and has the formula C 48 H 78 O 20 The appearance shape of the polymer was white crystals with respect to the molecular mass 975.13. Madecassoside is the secondary plant metabolite of saponins synthesized by centella asiatica by the isoprene pathway. Studies show that madecassoside plays a very good role in moisturizing, resisting oxidation, promoting wound healing and the like in solving skin problems.
The wound-healing villus flowers are derived from south Europe, and are spread throughout Europe, western Asia, north Africa and the United states, and can adapt to different climates and soils. The medicine is suitable for relieving spasm, detoxication, reducing swelling, promoting wound healing and the like in the field of medicine; in the field of skin care, the extract of flowers from the group of fluffy flowers (ANTHYLLIS VULNERARIA) for wound healing has astringent, anti-irritant, antibacterial, soothing and antioxidant effects due to the tannins and flavonoids. CN 111150689A discloses a composition containing wound-healing villus flowers and application thereof, mainly emphasizing the effects of moisturizing, antioxidation and repairing the skin; CN 114224799A discloses a composition for anti-allergic repair and a preparation method thereof, which mainly has the anti-irritation repair effect on sensitive muscles. At present, no nursing product for safely removing acnes exists.
Disclosure of Invention
Aiming at the defects existing in the prior art, the invention aims to provide the centella asiatica compound with the efficacy of safely dispelling acnes before puberty, and the centella asiatica compound is used in a raw material formula of a nursing product for preventing skin acnes by combining the treatment of downy flowers with octanoylglycine, so that the centella asiatica compound has multiple effects of resisting bacteria, resisting inflammation, relieving and repairing and balancing grease. To achieve the above objects and other advantages in accordance with the purpose of the present invention, there is provided a centella asiatica compound having a safe acne-removing effect against prepubertal stage, comprising:
madecassoside, a wound healing villus and octanoylglycine, a pH regulator and water;
the hair-wound treating flower comprises hair-wound treating flower extract and auxiliary materials, wherein the auxiliary materials comprise glycerol, water, potassium sorbate, sodium citrate, citric acid and sodium metabisulfite.
Preferably, the following components in parts by weight: 5-30 parts of madecassoside, 25-60 parts of a wound-healing erigeron breviscapus, 10-45 parts of octanoylglycine, 8-25 parts of a PH regulator and the balance of water.
Preferably, the content of the hydroxy asiaticoside is preferably 7-15 parts by weight.
Preferably, the octanoylglycine is 15-30 parts by mass.
Preferably, the PH regulator is 10-15 parts by weight of arginine.
Preferably, in the hair and flower injury treatment, the weight parts of glycerin are more than 20, water is 10-20, potassium sorbate is 0.2, sodium citrate is 0.16, citric acid is 0.08, sodium metabisulfite is 0.008 and the hair and flower injury treatment extract is 2-4.
Preferably, the mass portion of the wound velvet flower is 25-55 portions.
Use of madecassoside, wound-healing villus and octanoylglycine in the preparation of skin care product for eliminating acne.
Compared with the prior art, the invention has the beneficial effects that: the madecassoside is used as a main component, and the madecassoside is combined with the octanoylglycine to be applied to the raw material formula of the nursing product for preventing skin acne, thereby having multiple effects of resisting bacteria, resisting inflammation, relieving and repairing and balancing grease.
Drawings
FIG. 1 is a graph comparing the soothing effects of 1% of the formulation of example 1 of the centella asiatica compound having a safe acne-removing effect against pre-pubertal according to the present invention;
FIG. 2 is a bar graph of the soothing effect of 1% of the formulation addition concentration of example 1 for a pre-pubertal centella asiatica compound with safe acne-dispelling efficacy according to the present invention;
FIG. 3 is a graph comparing the soothing effects of the formulation of example 2 with 1.5% additive concentration for the pre-pubertal centella asiatica compound having a safe acne-dispelling efficacy according to the present invention;
FIG. 4 is a bar graph of the soothing effect of the formulation of example 2 with an additive concentration of 1.5% for a pre-pubertal centella asiatica compound with safe anti-acne efficacy according to the present invention;
FIG. 5 is a graph comparing the soothing effects of 2% of the formulation of example 3 of the centella asiatica compound having a safe acne-removing effect against pre-pubertal according to the present invention;
fig. 6 is a bar graph of the soothing effect of 2% of the formulation addition concentration of example 3 for the pre-pubertal centella asiatica compound with safe acne-dispelling efficacy according to the present invention.
Fig. 7 is a typical graph of oil control efficacy of the centella asiatica compound according to the present invention for pre-pubertal safety anti-acne efficacy.
Detailed Description
The following description of the embodiments of the present invention will be made clearly and completely with reference to the accompanying drawings, in which it is apparent that the embodiments described are only some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Referring to fig. 1-6, a centella asiatica compound having a safe acne-removing effect for pre-pubertal use, comprising: madecassoside, a wound healing villus and octanoylglycine, a pH regulator and water;
the hair-wound treating flower comprises hair-wound treating flower extract and auxiliary materials, wherein the auxiliary materials comprise glycerol, water, potassium sorbate, sodium citrate, citric acid and sodium metabisulfite;
the PH regulator is one of arginine, triethanolamine, aminopropanediol or sodium hydroxide.
Further, the following components in parts by weight: 5-30 parts of madecassoside, 25-60 parts of a wound-healing erigeron breviscapus, 10-45 parts of octanoylglycine, 8-25 parts of a PH regulator and the balance of water.
Further, the content of the hydroxy asiaticoside is preferably 7-15 parts by mass.
Further, the octanoylglycine is preferably 15 to 30 parts by mass.
Further, the pH regulator is preferably arginine in an amount of 10 to 15 parts by mass.
Further, in the hair and flower injury treatment, the weight parts of glycerin are more than 20, water is 10-20, potassium sorbate is 0.2, sodium citrate is 0.16, citric acid is 0.08, sodium metabisulfite is 0.008 and the hair and flower injury treatment extract is 2-4.
Further, the mass portion of the wound velvet flower is preferably 25-55 portions.
Use of madecassoside, wound-healing villus and octanoylglycine in the preparation of skin care product for eliminating acne.
1. Antibacterial efficacy test
1. Detecting items: antibacterial rate: propionibacterium acnes CICC 10312
2. The detection basis and method are as follows: WS/T650-2019 quantitative antibacterial test of suspension 5.1.1 of antibacterial and bacteriostatic Effect evaluation method
Example 1, sample No. 3-1 formulation addition concentration 1.0%
Detection result:
Test Resultw
note that: WS/T650-2019 method for evaluating antibacterial and bacteriostatic Effect specifies: the antibacterial rate is more than or equal to 50-90%, and the product has antibacterial effect; the bacteriostasis rate is more than or equal to 90%, and the product has stronger bacteriostasis effect.
Example 2, sample No. 3-2 formulation addition concentration 1.5%
Detection result:
TTest Results
note that: WS/T650-2019 method for evaluating antibacterial and bacteriostatic Effect specifies: the antibacterial rate is more than or equal to 50-90%, and the product has antibacterial effect; the bacteriostasis rate is more than or equal to 90%, and the product has stronger bacteriostasis effect.
Example 3, sample No. 3-3 formulation addition concentration 2.0%
Detection result:
Test Results
note that: WS/T650-2019 method for evaluating antibacterial and bacteriostatic Effect specifies: the antibacterial rate is more than or equal to 50-90%, and the product has antibacterial effect; the bacteriostasis rate is more than or equal to 90 percent,
the product has strong antibacterial effect.
2. Test of soothing and repairing efficacy
1. Test items: relief efficacy: test for inhibition of neutrophil aggregation of zebra fish embryo
2. The testing method comprises the following steps: and (3) testing by using a model of neutrophil aggregation caused by copper sulfate-induced nerve dome cell injury in the embryonic side line region of the zebra fish. 24 fish embryos are exposed to 0.16mg/L copper sulfate pentahydrate and 5g/L sample solution, a model control group is set at the same time, the fish embryos are fixed and sudan black stained after 40min of exposure, and the number of neutrophils in the lateral line area is counted and statistically analyzed.
Example 1: sample No. 3-1 formulation addition concentration 1.0%
Test results:
as shown in fig. 1-2, model control group: the relieving effect is not obvious, and the inhibition rate of neutrophil aggregation is 0%; positive control group examples: 0.0036mg/L indomethacin, and the relieving effect is obvious and the inhibition rate of neutrophil aggregation is 30 percent; test sample group: VI-20221503S-8complex plus 4 (3-1.0%) -relieving effect is remarkable, and the inhibition rate of neutrophil aggregation is 44%.
Example 2: sample No. 3-2 formulation addition concentration 1.5%
Test results
As shown in fig. 3-4, model control group: the relaxing effect is not obvious, namely the inhibition rate of neutrophil aggregation is 0%; positive control group examples: 0.0036mg/L indomethacin, and the relieving effect is obvious and the inhibition rate of neutrophil aggregation is 30 percent; test sample group: VI-20221503S-9complex plus 4 (3-2.5%), the relieving effect is remarkable and the inhibition rate of neutrophil aggregation is 41%.
Example 3: sample No. 3-2 formulation addition concentration 1.5%
Test results
As shown in fig. 5-6, model control group: the relaxing effect is not obvious, namely the inhibition rate of neutrophil aggregation is 0%; positive control group examples: 0.0036mg/L indomethacin, and the relieving effect is obvious and the inhibition rate of neutrophil aggregation is 30 percent; test sample group: VI-20221503S-10complex plus 4 (3-3.0%), the relieving effect is remarkable-46% of neutrophil aggregation inhibition rate.
3. Cell oil control efficacy test
The method comprises the following steps: human sebaceous gland cell oil control efficacy test method
The method comprises the following steps: research on active ingredient separation and inhibition of human sebaceous gland cell androgen synthesis mechanism of coptis chinensis
System and sample size:
test system: human sebaceous gland cells (SZ 95).
Cell algebra: at least 2 generations.
Cell mass per well: 1X 105 cells/well.
The principle method comprises the following steps: sebaceous glands are the source and target tissues of androgens, in which dehydroepiandrosterone is produced, which are converted, catalyzed by a series of enzymes, to the most active testosterone and 5α -dihydrotestosterone, which stimulate the synthesis of lipids and the proliferation and differentiation of sebaceous gland cells. In this proliferation and differentiation process, undifferentiated naive cells first proliferate, and part of the daughter cells form lipid droplets and move toward the center of glandular cells, gradually developing and differentiating into mature sebaceous gland cells capable of accumulating and secreting lipids. The experiment detects the amount of grease secreted by sebaceous gland cells by a nile red staining method, so as to judge whether a sample has the oil control effect.
The experimental steps are as follows:
1. cells were seeded in six well plates (1×105 cells/well), incubated at 37 ℃ with 5% CO2 for 24h.
2. After the incubation, the normal control group is replaced by fresh medium, the model control group contains testosterone medium, the sample control group contains sample with corresponding concentration and testosterone medium, and the sample control group is incubated for 24 hours at 37 ℃ with 5% CO 2.
3. After the incubation, the medium was removed, pre-chilled D-Hank's washed 3 times, fixed with 60% isopropyl alcohol, D-Hank's washed, nile red stained, photographed with a fluorescence microscope, and Image J analyzed the fluorescence intensity of each group.
A: fluorescence intensity
Applicability and limitations:
the method is suitable for testing the oil control efficacy of cosmetics and raw materials thereof, and requires that a sample can be dissolved in water or prepared into a suspension which can be uniformly dispersed in water.
Judging the basis:
statistical analysis p <0.05, judged significant differences.
Example, sample number complex plus 4 (3-1.0%) formulation addition concentration 1.0%
Test results:
a typical graph of the oil control efficacy of this sample is shown in fig. 7 below.
The fluorescence intensity of the sample numbered complex plus 4 (3-1.0%) was observed to be significantly reduced compared to the model control group, revealing that the sample had oil control efficacy.
Conclusion:
under the experimental conditions, the sample complex plus 4 has the oil control effect.
The number of devices and the scale of processing described herein are intended to simplify the description of the invention, and applications, modifications and variations of the invention will be apparent to those skilled in the art.
Although embodiments of the present invention have been disclosed above, it is not limited to the details and embodiments shown and described, it is well suited to various fields of use for which the invention would be readily apparent to those skilled in the art, and accordingly, the invention is not limited to the specific details and illustrations shown and described herein, without departing from the general concepts defined in the claims and their equivalents.
Claims (8)
1. A centella asiatica compound with a safe acne removing effect for prepubertal period, which is characterized by comprising the following components:
madecassoside, a treatment for injury to villus flowers, octanoylglycine, a pH regulator, water;
the hair-wound treating flower comprises hair-wound treating flower extract and auxiliary materials, wherein the auxiliary materials comprise glycerol, water, potassium sorbate, sodium citrate, citric acid and sodium metabisulfite;
the PH regulator is one of arginine, triethanolamine, aminopropanediol or sodium hydroxide.
2. The centella asiatica compound with a safe acne-removing effect for prepubertal according to claim 1, wherein the centella asiatica compound is characterized by comprising the following components in parts by mass: 5-30 parts of madecassoside, 25-60 parts of a wound-healing erigeron breviscapus, 10-45 parts of octanoylglycine, 8-25 parts of a PH regulator and the balance of water.
3. The centella asiatica compound having a safe acne-removing effect against prepubertal according to claim 2, wherein the amount of the hydroxy centella asiatica glycoside is preferably 7-15 parts by mass.
4. The centella asiatica compound having a safe acne-removing effect against prepubertal according to claim 2, wherein the amount of octanoylglycine is preferably 15-30 parts by mass.
5. The centella asiatica compound having a safe acne-removing effect against prepubertal according to claim 1, wherein the PH adjuster is preferably arginine 10-15 parts by mass.
6. The centella asiatica compound with a safe acne-removing effect for prepubertal according to claim 2, wherein in the wound-healing villus flowers, the glycerol is more than 20, the water is 10-20, the potassium sorbate is 0.2, the sodium citrate is 0.16, the citric acid is 0.08, the sodium metabisulfite is 0.008 and the wound-healing villus flower extract is 2-4 in parts by weight.
7. The centella asiatica compound having a safe acne-removing effect for prepubertal according to claim 6, wherein the mass fraction of the wound fluff flower is preferably 25-55 parts.
8. Use of madecassoside, wound-healing villus and octanoylglycine in the preparation of skin care product for eliminating acne.
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| Application Number | Priority Date | Filing Date | Title |
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| CN202211428790.4A CN116019731A (en) | 2022-11-15 | 2022-11-15 | Herba centellae compound with safety acne removing effect for prepubertal period |
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| CN202211428790.4A CN116019731A (en) | 2022-11-15 | 2022-11-15 | Herba centellae compound with safety acne removing effect for prepubertal period |
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| FR2962333A1 (en) * | 2010-07-12 | 2012-01-13 | Oreal | Composition, useful for the preventive and/or curative treatment of skin disorders caused by Propionibacterium acnes e.g. acne and rosacea, comprises 2-alkoxy-4-alkylketone phenol compound and essential oil in medium |
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2022
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