CN115969911A - Traditional Chinese medicine composition with anti-anxiety effect and preparation method and application thereof - Google Patents
Traditional Chinese medicine composition with anti-anxiety effect and preparation method and application thereof Download PDFInfo
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Abstract
The invention discloses a traditional Chinese medicine composition with an antianxiety effect, and a preparation method and application thereof, and relates to the technical field of traditional Chinese medicine preparations. The invention is prepared by adding nacre, fried spina date seed, bran-fried bighead atractylodes rhizome, dandelion, salvia miltiorrhiza and the like on the basis of vinegar-fried bupleurum chinense, raw white paeony root and bran-fried immature bitter orange, wherein the nacre in the formula has the effects of calming the nerves and arresting convulsion, and calming the liver and suppressing yang; the spina date seed has the effects of nourishing heart, tonifying liver, calming heart and soothing nerves; the dandelion can clear away the fire and heat from the liver channel, and the salvia miltiorrhiza can clear away the heart fire and cool the blood, relieve restlessness and calm the nerves; the bran-fried bighead atractylodes rhizome has the effects of tonifying spleen and qi, protecting spleen and stomach, and soothing liver, relieving depression, clearing liver and reducing fire. The results of open field experiments, elevated plus maze experiments and CORT content in mouse serum show that the traditional Chinese medicine composition has obvious anxiolytic effect.
Description
Technical Field
The invention belongs to the technical field of traditional Chinese medicine preparations, and particularly relates to a traditional Chinese medicine composition with an anxiolytic effect, and a preparation method and application thereof.
Background
Generalized anxiety disorder is a neurological disorder with anxiety as the main manifestation, and is one of the most common mental disorders in clinic. It is often manifested as serious anxiety, stress and fear, accompanied by autonomic dysfunction such as palpitation, sweating, trembling hands, frequent urination, etc. Epidemiological survey of the national mental disorder published in 2019 shows that the lifetime prevalence rate of the generalized anxiety disorder is 0.3%, and the generalized anxiety disorder often coexists with various chronic diseases, and seriously affects the social functions of patients. Sertraline, paroxetine and escitalopram are first-line treatment medicines for generalized anxiety disorder, but pure anxiolytic medicines have poor curative effect, slow drug effect and narrow treatment spectrum, about one third of patients cannot be relieved, residual symptoms exist for a long time, adverse reactions such as somnolence, gastrointestinal discomfort and the like are obvious, and the life quality of the patients is reduced. Therefore, it is important to find and develop some anxiolytic drugs with good curative effect and less adverse reactions.
Master thesis (mind tranquilizing prescription ' curative effect and influence on HAMA score of adult's curative effect on primary insomnia ', quality of quality) studies the insomnia treating and anxiety resisting effects of the ' mind tranquilizing prescription ' proved by Chengbaotian education, the ' mind tranquilizing prescription ' is prepared from 10 parts of radix Scutellariae, ramulus Cinnamomi, radix Polygalae, radix Codonopsis, rhizoma Pinelliae Preparata and radix Glycyrrhizae, 15 parts of radix Paeoniae alba and semen Ziziphi Spinosae, 20 parts of cortex Albizziae, 24 parts of radix bupleuri, 30 parts of Concha Ostreae, 30 parts of Os Draconis, 70 parts of caulis Polygoni Multiflori and 7 Chinese dates. The application method comprises the following steps: decoct the drugs with water, take the decoction 1h before sleep, and take the decoction 1 day. Research shows that compared with the traditional treatment based on syndrome differentiation, the nerve-soothing prescription group developed by the professor Chen Baodan not only has obvious curative effect on the primary insomnia patients, but also can obviously reduce the anxiety symptoms of the patients, has better effect compared with the treatment based on syndrome differentiation, and can be related to the improvement of the anxiety symptoms with the improvement of the sleep quality. And the medicament has no obvious influence on liver and kidney functions, and all cases have no complications in the medicament taking process, so that the tranquilizer is safe and reliable when continuously taken for 3 months.
A journal paper (the experiment of treating anxiety and depression by using heart nourishing decoction and radix bupleuri and white paeony root, and the academic conference proceedings of the medical and medical society of heart diseases in china in 2014), which utilizes the heart nourishing decoction and the radix bupleuri and white paeony root, has good effect on treating heart diseases and resisting anxiety and depression.
A journal paper (observation of curative effect of depression-resolving and blood-activating method for treating angina pectoris with anxiety and depression, liu Guo Ying, shanxi Chinese medicine, 34 th vol. No. 10) discusses the clinical curative effect of depression-resolving and blood-activating method for patients with coronary heart disease and angina pectoris with anxiety and depression symptoms. The method comprises the following steps: the control group is administered with conventional western medicines such as XIAOXINTONG, BAIYINAN, and simvastatin, and is administered with Daigoxin as anxiolytic and antidepressant medicines, and the patients in the treatment group are administered with Chinese medicines (bupleuri radix, radix Paeoniae Rubra, radix Paeoniae alba, rhizoma Ligustici Chuanxiong, notoginseng radix, rhizoma Cyperi, and cortex Albizziae, etc.) for resolving stagnation and promoting blood circulation. The results show that the total effective rate of the treatment group is 91.4 percent, which is obviously higher than 71.4 percent of the control group, and the difference has statistical significance (P is less than 0.05); the SAS and SDS scores of two groups of patients are reduced compared with those before treatment, but the indexes of the treatment group of patients are obviously lower than those of a control group after treatment, the difference has statistical significance (P is less than 0.05), and the effects of soothing liver-qi stagnation, promoting blood circulation to remove meridian obstruction, enriching blood and nourishing blood can be achieved by combining the medicines in the prescription.
The patent (CN 108686133A) provides a traditional Chinese medicine for improving postoperative anxiety state of thyroid cancer patients, which comprises the following components: radix bupleuri, radix scutellariae, pericarpium citri reticulatae, pinellia ternate, caulis bambusae in taeniam, fructus aurantii immaturus, radix gentianae, fructus gardeniae, semen cassiae, chrysanthemum, radix paeoniae alba, radix rehmanniae, caulis polygoni multiflori, magnetite, mother of pearl, nodus nelumbinis rhizomatis, leech, centipede, spina gleditsiae, pepper, liquorice, selfheal, seaweed, poria cocos and rhizoma atractylodis macrocephalae. The prepared pure traditional Chinese medicine is prepared, has the effects of clearing liver and purging fire, and activating blood and dissolving stasis, prevents and delays the recurrence and metastasis of thyroid cancer, and is an innovation of postoperative rehabilitation medicines for thyroid cancer.
Therefore, the traditional Chinese medicine has feasibility for treating anxiety neurosis, but the traditional Chinese medicine preparation in the prior art still has unsatisfactory curative effect on the aspect of antianxiety. Therefore, it is necessary to provide a Chinese medicinal composition with good curative effect and less adverse reactions and having an anxiolytic effect.
Disclosure of Invention
The invention provides a traditional Chinese medicine composition with an anxiolytic effect, and a preparation method and application thereof, aiming at the problems in the prior art. The invention is prepared by adding nacre, fried spina date seed, bran-fried bighead atractylodes rhizome, dandelion, salvia miltiorrhiza and the like on the basis of vinegar-fried bupleurum, raw white paeony root and bran-fried immature bitter orange, wherein the nacre in the formula has the effects of calming the nerves and arresting convulsion, and calming the liver and suppressing yang; the spina date seed has the effects of nourishing heart and liver, calming heart and soothing nerves; the dandelion can clear away the fire and heat from the liver channel, and the salvia miltiorrhiza can clear away the heart fire and cool the blood, relieve restlessness and calm the nerves; the bran-fried bighead atractylodes rhizome has the effects of tonifying spleen and qi, protecting spleen and stomach, and soothing liver, relieving depression, clearing liver and reducing fire. The results of open field experiments, elevated plus maze experiments and CORT content in mouse serum show that the traditional Chinese medicine composition has obvious anxiolytic effect.
In order to achieve the purpose, the technical scheme adopted by the invention is as follows:
in a first aspect, the invention provides a traditional Chinese medicine composition with an antianxiety effect, which comprises the following components in parts by weight:
8-12 parts of vinegar-processed bupleurum chinense, 8-12 parts of raw white peony root, 5-7 parts of bran-fried immature bitter orange, 16-24 parts of mother-of-pearl, 12-18 parts of fried spina date seed, 4-8 parts of bran-fried bighead atractylodes rhizome, 4-8 parts of dandelion and 8-12 parts of salvia miltiorrhiza.
In a preferred embodiment, the mass ratio of the vinegar-processed bupleurum chinense, the nacre and the fried spina date seed is 10:20:15.
the traditional Chinese medicine composition with the antianxiety effect (namely, the traditional Chinese medicine composition in the embodiment 1) consists of 8 medicines of vinegar-processed bupleurum chinense, raw white paeony root, bran-fried immature bitter orange, nacre, fried spina date seed, bran-fried bighead atractylodes rhizome, dandelion and salvia miltiorrhiza, and is a clinically proved formula. In the formula, the monarch drug bupleurum root is used for soothing liver and relieving depression and expelling pathogenic factors; peony is used as a minister to nourish blood, soften liver and nourish liver; the assistant drug, immature bitter orange, regulates qi and relieves depression, purges heat and dissipates stagnation, ascends and descends with the radix bupleuri to regulate qi movement; the liquorice root, a guiding drug, is harmonized with the middle warmer and is in slow urgency, and the four drugs play roles of expelling pathogenic factors and relieving depression, soothing liver and regulating qi. The traditional Chinese medicine composition of the embodiment 1 is prepared by adding nacre, fried spina date seed, bran-fried bighead atractylodes rhizome, dandelion, salvia miltiorrhiza and the like on the basis of vinegar-fried bupleurum chinense, raw white paeony root and bran-fried immature bitter orange, wherein the nacre is used for calming the nerves and arresting convulsion and calming the liver and suppressing yang; the spina date seed has the effects of nourishing heart, tonifying liver, calming heart and soothing nerves; the dandelion can clear away the fire and heat from the liver channel, and the salvia miltiorrhiza can clear away the heart fire and cool the blood, relieve restlessness and calm the nerves; the bran-fried bighead atractylodes rhizome has the effects of tonifying spleen and qi, protecting spleen and stomach, soothing liver, relieving depression, clearing liver and reducing fire, and has a good anti-anxiety effect.
In a preferred embodiment, the traditional Chinese medicine composition with the anti-anxiety function is prepared by the following formula in parts by weight:
10 parts of vinegar-processed bupleurum chinense, 10 parts of raw white paeony root, 6 parts of bran-fried immature bitter orange, 20 parts of mother-of-pearl, 15 parts of fried spina date seed, 6 parts of bran-fried bighead atractylodes rhizome, 6 parts of dandelion and 10 parts of salvia miltiorrhiza.
In a second aspect, the present application further provides a preparation method of the above traditional Chinese medicine composition with an anxiolytic effect, comprising the following steps:
weighing the medicinal materials according to the formula, adding water, decocting, filtering, and concentrating the decoction.
In a preferred embodiment, 4-10 times of water is added for decocting for 2-5 times, the decoction is combined, and the mixture is concentrated to obtain the traditional Chinese medicine.
In a third aspect, the invention provides an application of the traditional Chinese medicine composition with an anxiolytic effect in preparing an anxiolytic medicine.
In a preferred embodiment, the dosage form of the anxiolytic is selected from the group consisting of decoction, granules, oral liquid, tablets and capsules.
In a preferred embodiment, the Chinese medicinal composition is added with a proper amount of auxiliary materials and prepared into granules.
In a preferred embodiment, the excipient is one or more of a filler, a binder, a wetting agent, a disintegrant, a lubricant, and a film coating material.
In a preferred embodiment, the traditional Chinese medicine composition is added with a proper amount of preparation auxiliary materials to prepare an oral liquid.
In a preferred embodiment, the formulation excipients are one or more of solvents, fragrances, flavoring agents, clarifiers, and preservatives.
In a preferred embodiment, the traditional Chinese medicine composition is added with a proper amount of auxiliary materials to be prepared into tablets, and the auxiliary materials are one or more of diluents, binders, lubricants and disintegrants.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention is prepared by adding nacre, fried spina date seed, bran-fried bighead atractylodes rhizome, dandelion, salvia miltiorrhiza and the like on the basis of vinegar-fried bupleurum, raw white paeony root and bran-fried immature bitter orange, wherein the nacre in the formula has the effects of calming the nerves and arresting convulsion, and calming the liver and suppressing yang; the spina date seed has the effects of nourishing heart, tonifying liver, calming heart and soothing nerves; the dandelion can clear away the fire and heat from the liver channel, and the salvia miltiorrhiza can clear away the heart fire and cool the blood, relieve restlessness and calm the nerves; the bran-fried bighead atractylodes rhizome has the effects of tonifying spleen and qi, protecting spleen and stomach, soothing liver, relieving depression, clearing liver and reducing fire, and has a good anti-anxiety effect.
2. The invention constructs an anxiety model by using a chronic constraint stress method, evaluates the anxiolytic effect of a medicament by an open field experiment, an elevated plus maze experiment and the CORT content in the serum of a mouse, and shows that the central activity time of the mouse in an administration group is obviously increased, the proportion OE% and the proportion OT% of the time for the mouse to enter an open arm are both increased, the effect is more obvious under medium and high dose, the CORT content in the serum of the mouse is obviously reduced, and the results all show that the traditional Chinese medicine composition has obvious anxiolytic curative effect.
Detailed Description
It should be noted that the raw materials used in the present invention are all common commercial products, and the sources thereof are not particularly limited.
Example 1 preparation of a Chinese medicinal composition having an anxiolytic effect
10g of vinegar bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 20g of mother-of-pearl, 15g of fried spina date seed, 6g of bran-fried bighead atractylodes rhizome, 6g of dandelion and 10g of salvia miltiorrhiza, adding 6 times of water for decocting, decocting for 3 times, extracting for 1 hour each time, combining decoction, concentrating, and drying under reduced pressure to obtain the extract.
Further, the decoction is combined and prepared into granules.
The specific process comprises the following steps: 10g of vinegar bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 20g of mother-of-pearl, 15g of fried spina date seed, 6g of bran-fried bighead atractylodes rhizome, 6g of dandelion and 10g of salvia miltiorrhiza are taken, 6 times of water is added for decocting, 3 times of decoction are carried out, 1 hour of extraction is carried out each time, decoction liquid is filtered, filtrate is combined and concentrated to relative density of 1.21-1.25 (55 ℃), a proper amount of dextrin is added, granules are prepared, and the Chinese medicinal preparation is dried.
Example 2 preparation of a Chinese medicinal composition having an anxiolytic effect
8g of vinegar-processed bupleurum chinense, 12g of raw white paeony root, 7g of bran-fried immature bitter orange, 16g of mother-of-pearl, 12g of fried spina date seed, 8g of bran-fried bighead atractylodes rhizome, 8g of dandelion and 12g of salvia miltiorrhiza are taken, 8 times of water is added for decoction, the decoction is carried out for 3 times, the extraction is carried out for 1 hour each time, the decoction liquids are combined, and the extract is obtained by decompression and drying after the concentration.
Example 3 preparation of a Chinese medicinal composition having an anxiolytic effect
12g of vinegar bupleurum chinense, 8g of raw white paeony root, 5g of bran-fried immature bitter orange, 24g of mother-of-pearl, 18g of fried spina date seed, 4g of bran-fried bighead atractylodes rhizome, 4g of dandelion and 8g of salvia miltiorrhiza, adding 10 times of water for decocting, decocting for 2 times, extracting for 1 hour each time, combining decoction, concentrating, and drying under reduced pressure to obtain the extract.
Comparative example 1
According to the prescription of journal literature ("Wenzhi Tang plus experience of treating insomnia for Wenzhi for Wenzli", jindi et al, hubei J. TCM, 35, vol. 11, page 28), the traditional Chinese medicine composition granules are prepared according to the process of the embodiment 1 of the invention. The method comprises the following specific steps:
10g of radix bupleuri, 10g of rhizoma pinellinae praeparata, 10g of radix scutellariae, 10g of caulis bambusae in taeniam, 20g of immature bitter orange, 10g of angelica sinensis, 10g of ligusticum wallichii, 10g of radix paeoniae alba, 10g of radix rehmanniae, 20g of caulis polygoni multiflori, 30g of spina date seed, 10g of ground beetle, 10g of safflower, 30g of radix salviae miltiorrhizae, 15g of calamus, 10g of polygala tenuifolia, 10g of mother-of-pearl, 10g of cortex moutan and 10g of fried gardenia, and the medicinal materials are taken, decocted by adding 6 times of water, decocted for 3 times, extracted for 1 hour each time, the decocted liquid is combined, concentrated and dried under reduced pressure to obtain the extract.
Comparative example 2
The prescription is as follows: 10g of vinegar-processed bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 0g of mother-of-pearl, 0g of fried spina date seed, 23.5g of bran-fried bighead atractylodes rhizome, 23.5g of dandelion and 10g of salvia miltiorrhiza as in example 1; concentrating, and drying under reduced pressure to obtain extract.
Comparative example 3
The prescription is as follows: 10g of vinegar-processed bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 27g of mother-of-pearl, 20g of fried spina date seed, 0g of bran-fried bighead atractylodes rhizome, 0g of dandelion and 10g of salvia miltiorrhiza, and the rest is the same as in example 1; concentrating, and drying under reduced pressure to obtain extract.
Comparative example 4
The prescription is as follows: 10g of vinegar-processed bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 35g of mother-of-pearl, 0g of fried spina date seed, 6g of bran-fried bighead atractylodes rhizome, 6g of dandelion and 10g of salvia miltiorrhiza, and the rest is the same as in example 1; concentrating, and drying under reduced pressure to obtain extract.
Comparative example 5
The prescription is as follows: 10g of vinegar-processed bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 0g of mother-of-pearl, 35g of fried spina date seed, 6g of bran-fried bighead atractylodes rhizome, 6g of dandelion and 10g of salvia miltiorrhiza, and the rest is the same as in example 1; concentrating, and drying under reduced pressure to obtain extract.
Comparative example 6
The prescription is as follows: 10g of vinegar-processed bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 20g of mother-of-pearl, 15g of fried spina date seed, 0g of bran-fried bighead atractylodes rhizome, 12g of dandelion and 10g of salvia miltiorrhiza, and the rest is the same as in example 1; concentrating, and drying under reduced pressure to obtain extract.
Comparative example 7
The prescription is as follows: 10g of vinegar-processed bupleurum chinense, 10g of raw white paeony root, 6g of bran-fried immature bitter orange, 20g of mother-of-pearl, 15g of fried spina date seed, 12g of bran-fried bighead atractylodes rhizome, 0g of dandelion and 10g of salvia miltiorrhiza, and the rest is the same as in example 1; concentrating, and drying under reduced pressure to obtain extract.
TABLE 1 formulations of examples 1-3 and comparative examples 1-7
Test examples
The anxiolytic effect of examples 1-3 and comparative examples 1-7 was verified as follows:
method and material
1.1 Main instruments and reagents
A multifunctional microplate reader, a high speed refrigerated centrifuge (thermo fisher scientific, usa); fluoxetine hydrochloride capsules (Suzhou Zhonghua pharmaceuticals industries, ltd., batch number: H20064844); mouse CORTELISA kit (Wuhan Irelet Biotech, inc., cat. E-OSEL-M0001)
1.2 model preparation and administration
The experiment adopts a chronic constraint stress method to construct an anxiety model. The specific molding treatment method comprises the following steps: a small vent hole is drilled at the bottom of a 50mL centrifuge tube to manufacture a binding device, and a hole arm is smooth as much as possible during punching so as to avoid unnecessary injury to a mouse and increase uncertain factors of an experiment. And (3) placing the mouse into a processed centrifugal tube for a fixed time every day to receive constraint stress for 3h, screwing a tube cover after the mouse is placed into the centrifugal tube, horizontally placing and fixing the centrifugal tube, keeping an air hole smooth, and continuously molding for 7d.
Daily administration one hour before restraint stress, normal and model groups of rats (8 per group)Only) gavage of 0.9% sodium chloride solution, the low dose groups of the extracts of examples 1-3 and comparative examples 1-7 were 3.15 g.crude drug kg -1 ·d -1 Intragastric administration, wherein the dosage of the composition is 6.29g crude drug/kg -1 ·d -1 The high-dose group for gastric lavage is 12.59g crude drug kg -1 ·d -1 Gavage, 3 mg-kg of fluoxetine hydrochloride group -1 ·d -1 And (5) performing intragastric administration.
1.3 behavioural testing
1.3.1 open field experiments
Experiment eight days open field experiments were performed. The experimental device consists of an open field reaction box and an automatic data acquisition and processing system, wherein the area of the bottom of the open field reaction box is 1m 2 Divided into 4 zones, each zone in turn comprising a peripheral zone and a central zone. In a quiet environment, the mice are respectively placed in the centers of the bottom surfaces of the 4 areas, a video camera above the reaction box is used for shooting and timing, and the staying time of the mice entering the central area within 5min is observed. Animal activity was recorded throughout using the ANY-maze animal behavioral analysis system. After the experiment, the animals were taken out, and the test area was sprayed with 10% ethanol to remove the residual excreta and odor of the animals.
1.3.2 elevated plus maze experiment
The elevated plus maze experiment was performed immediately after the open field experiment was completed. Before the experiment, the mouse is placed in an open field to move for 5min, then the mouse is placed in the center of the maze, the head of the mouse is opposite to one of the open arms, the movement of the mouse on the elevated plus maze is tracked and recorded by a computer, and the recording time is 5min. The percentage of open arm entry times (OE%) of mice, i.e., open arm entry times/(open arm entry times + closed arm entry times) × 100%, was used as an index for evaluating the anxiolytic effect of the drug.
1.4 detection of CORT content by ELISA
The materials were taken after the behavioural experiment was completed. Collecting blood by picking eyeball, centrifuging at 4 deg.C and 5000rpm/min for 5min, and collecting supernatant to obtain solution to be detected; and (4) determining the content of CORT in the solution to be determined according to the ELISA kit specification.
1.5 statistical treatment
Statistical analysis was performed using GraphPadPrism8 and plotted. What is needed isHas statistical data to
S represents, single-factor variance analysis is adopted for comparison among groups, and pairwise comparison is adoptedtInspection, P<A difference of 0.05 is statistically significant.
2 results
2.1 Effect of Chinese medicinal composition on open field test index
The central activity time of the model group mice was significantly reduced compared to the normal group (P<0.01 ); compared with the model group, the central activity time of the dose group, the high dose group and the fluoxetine group in the extracts of examples 1-3 is obviously increased, and the low dose group of the extracts of examples 1-3 has an increased trend, but the difference is not statistically significant; comparing examples 1-3 with comparative examples 1-7, it can be seen that the extracts of comparative examples 1-7 have substantially no improving effect on the central activity time of mice, and the extracts of examples 1-3 have significantly better effects than comparative examples 1-7, as shown in table 2.
Table 2 effect of traditional Chinese medicine composition on central activity time(s) (x ± s, n = 8)
Note: in comparison with the normal group, ** P<0.01; in comparison with the set of models, # P<0.05， ## P<0.01
2.2 influence of Chinese medicinal composition on elevated plus maze Experimental indices
The number of times the model group mice entered the open arm, the percentage of open arm dwell time (OE% and OT%) were significantly reduced compared to the normal group; compared with the model group, the OE% and OT% of the dose group, the high dose group and the fluoxetine group in the extracts of examples 1-3 are significantly increased, and the low dose group of the extracts of examples 1-3 has an increased trend, but the difference is not statistically significant; comparing examples 1-3 with comparative examples 1-7, it can be seen that the percentage improvement of the open arm entry times and open arm residence time of the mice by the extracts of comparative examples 1-7 is quite limited, and the extracts of examples 1-3 are significantly better than comparative examples 1-7, as shown in table 3.
TABLE 3 proportion of Chinese medicinal composition to number of times of entry into open arm and open arm time (%)
Influence of (x ± s, n = 8)
Note: in comparison with the normal group, ** P<0.01; in comparison with the set of models, # P<0.05， ## P<0.01
2.3 Effect of Chinese medicinal composition on CORT level
Compared with the normal group, the serum CORT content of the model group mice is obviously increased, and the difference has statistical significance (P)<0.05 ); compared with the model group, the serum CORT content of the mice of the traditional Chinese medicine composition group and the fluoxetine group in the examples 1-3 can be obviously reduced (both areP<0.05 ); comparing examples 1-3 with comparative examples 1-7, it can be seen that the serum CORT level of mice is also reduced to some extent after the extracts of comparative examples 1-7 are gavaged, but the reduction is significantly less than that of examples 1-3, see Table 4.
TABLE 4 combination of Chinese herbs on mouse serum CORT (ng. ML) -1 ) Influence of (x ± s, n = 8)
Note: in comparison with the normal group, * P<0.05; in comparison with the set of models, # P<0.05
in conclusion, it can be seen that the central activity time of the mice in the administration group (examples 1-3) is significantly increased, the proportion of the frequency of the mice entering the open arms OE% and the proportion of the time to the frequency of the mice entering the open arms OT% are both increased, the higher dosage is more significant, the CORT content in the serum of the mice is significantly reduced, and the above results show that the traditional Chinese medicine composition of the invention has significant anti-anxiety effect. As can be seen from example 1 and comparative examples 2 to 7, significant anxiolytic effects can be obtained when Concha Margaritifera, parched semen Ziziphi Spinosae, bran-parched rhizoma Atractylodis Macrocephalae, and herba Taraxaci are used together.
Finally, it should be noted that the above-mentioned contents are only used for illustrating the technical solutions of the present invention, and do not limit the protection scope of the present invention, and those skilled in the art can make simple modifications or equivalent substitutions on the technical solutions of the present invention without departing from the spirit and scope of the technical solutions of the present invention.
Claims (11)
1. The traditional Chinese medicine composition with the effect of resisting anxiety is characterized by comprising the following components in parts by weight:
8-12 parts of vinegar-processed bupleurum chinense, 8-12 parts of raw white peony root, 5-7 parts of bran-fried immature bitter orange, 16-24 parts of mother-of-pearl, 12-18 parts of fried spina date seed, 4-8 parts of bran-fried bighead atractylodes rhizome, 4-8 parts of dandelion and 8-12 parts of salvia miltiorrhiza.
2. The traditional Chinese medicine composition as claimed in claim 1, wherein the mass ratio of the vinegar-processed bupleurum chinense, the mother-of-pearl and the stir-fried spina date seed is 10:20:15.
3. the traditional Chinese medicine composition with the anxiolytic effect is characterized in that the traditional Chinese medicine composition with the anxiolytic effect is prepared by the following formula in parts by weight:
10 parts of vinegar-processed bupleurum chinense, 10 parts of raw white paeony root, 6 parts of bran-fried immature bitter orange, 20 parts of mother-of-pearl, 15 parts of fried spina date seed, 6 parts of bran-fried bighead atractylodes rhizome, 6 parts of dandelion and 10 parts of salvia miltiorrhiza.
4. A method for preparing the traditional Chinese medicine composition of any one of claims 1 to 3, which comprises the following steps:
weighing the medicinal materials according to the formula, adding water, decocting, filtering, and concentrating the decoction.
5. The preparation method according to claim 4, wherein the extract is obtained by adding 4 to 10 times of water and decocting for 2 to 5 times, combining the decoctions, and concentrating.
6. Use of the Chinese medicinal composition according to any one of claims 1 to 3 or the Chinese medicinal composition prepared by the preparation method according to any one of claims 4 to 5 in the preparation of an anxiolytic.
7. The use of claim 6, wherein the anxiolytic is in a dosage form selected from the group consisting of a decoction, a granule, an oral liquid, a tablet, and a capsule.
8. The use of claim 7, wherein the Chinese medicinal composition is prepared into granules with an appropriate amount of adjuvant.
9. The use of claim 8, wherein the excipient is one or more of a filler, a binder, a wetting agent, a disintegrant, a lubricant, and a film coating material.
10. The use of claim 7, wherein the Chinese medicinal composition is prepared into oral liquid by adding a proper amount of preparation auxiliary materials; the preparation adjuvants are one or more of solvent, aromatic, correctant, clarifier and antiseptic.
11. The use of claim 7, wherein the Chinese medicinal composition is formulated into a tablet with an appropriate amount of adjuvants, wherein the adjuvants are one or more of diluent, binder, lubricant and disintegrant.
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