CN115956120A - 用于crispr基因组编辑的修饰的指导rna - Google Patents

用于crispr基因组编辑的修饰的指导rna Download PDF

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Publication number
CN115956120A
CN115956120A CN202180047016.6A CN202180047016A CN115956120A CN 115956120 A CN115956120 A CN 115956120A CN 202180047016 A CN202180047016 A CN 202180047016A CN 115956120 A CN115956120 A CN 115956120A
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crrna
chemically modified
guide rna
tracrrna
rna
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埃里克·约瑟夫·森特海默
阿纳斯塔西娅·科沃罗娃
乔纳森·肯尼斯·沃斯
纳迪亚·阿姆拉尼
陈泽祥
马修·哈斯勒
迪马斯·埃切维里亚·莫伦诺
朱莉娅·弗朗西斯·奥尔特曼
斯科特·沃尔夫
山田健
吉塔利·黛妃
张汉
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University of Massachusetts Amherst
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University of Massachusetts Amherst
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    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2310/34—Spatial arrangement of the modifications
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    • C12N2310/00—Structure or type of the nucleic acid
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    • C12N2800/00—Nucleic acids vectors
    • C12N2800/80—Vectors containing sites for inducing double-stranded breaks, e.g. meganuclease restriction sites

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CN202180047016.6A 2020-05-12 2021-05-12 用于crispr基因组编辑的修饰的指导rna Pending CN115956120A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US202063023313P 2020-05-12 2020-05-12
US63/023,313 2020-05-12
PCT/US2021/032038 WO2021231606A2 (en) 2020-05-12 2021-05-12 Modified guide rnas for crispr genome editing

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CN115956120A true CN115956120A (zh) 2023-04-11

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US (1) US20210388348A1 (https=)
EP (1) EP4150091A4 (https=)
JP (1) JP2023526057A (https=)
CN (1) CN115956120A (https=)
AU (1) AU2021271004A1 (https=)
CA (1) CA3177463A1 (https=)
WO (1) WO2021231606A2 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2024053964A1 (ko) * 2022-09-06 2024-03-14 주식회사 툴젠 캄필로박터 제주니 유래 cas9의 가이드 rna 구조변화를 통한 유전자교정 향상 시스템
IL324634A (en) 2023-05-15 2026-01-01 Nchroma Bio Inc Compositions and methods for epigenetic regulation of hbv gene expression

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2015138510A1 (en) * 2014-03-10 2015-09-17 Editas Medicine., Inc. Crispr/cas-related methods and compositions for treating leber's congenital amaurosis 10 (lca10)
CN112877327B (zh) * 2014-12-18 2024-11-22 综合基因技术公司 基于crispr的组合物和使用方法
EP3250691B9 (en) * 2015-01-28 2023-08-02 Caribou Biosciences, Inc. Crispr hybrid dna/rna polynucleotides and methods of use
CN107709555A (zh) * 2015-05-15 2018-02-16 达尔马科恩有限公司 用于Cas9介导的基因编辑的合成的单向导RNA
EP4545544A3 (en) * 2015-06-29 2025-10-08 Ionis Pharmaceuticals, Inc. Modified crispr rna and modified single crispr rna and uses thereof
WO2017058751A1 (en) * 2015-09-28 2017-04-06 North Carolina State University Methods and compositions for sequence specific antimicrobials
CA2947904A1 (en) * 2015-11-12 2017-05-12 Pfizer Inc. Tissue-specific genome engineering using crispr-cas9
CA3034931A1 (en) * 2016-09-23 2018-03-29 Board Of Trustees Of Southern Illinois University Tuning crispr/cas9 activity with chemically modified nucleotide substitutions
AU2017374044B2 (en) * 2016-12-08 2023-11-30 Intellia Therapeutics, Inc. Modified guide RNAs
WO2018111946A1 (en) * 2016-12-12 2018-06-21 Integrated Dna Technologies, Inc. Genome editing detection
WO2019011118A1 (zh) * 2017-07-14 2019-01-17 苏州克睿基因生物科技有限公司 一种基因编辑系统及基因编辑的方法
WO2019183000A1 (en) * 2018-03-19 2019-09-26 University Of Massachusetts Modified guide rnas for crispr genome editing
KR20210045360A (ko) * 2018-05-16 2021-04-26 신테고 코포레이션 가이드 rna 설계 및 사용을 위한 방법 및 시스템
WO2019237069A1 (en) * 2018-06-08 2019-12-12 Intellia Therapeutics, Inc. Modified guide rnas for gene editing

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Publication number Publication date
WO2021231606A3 (en) 2021-12-16
JP2023526057A (ja) 2023-06-20
EP4150091A4 (en) 2025-07-23
CA3177463A1 (en) 2021-11-18
WO2021231606A2 (en) 2021-11-18
AU2021271004A1 (en) 2023-01-19
US20210388348A1 (en) 2021-12-16
EP4150091A2 (en) 2023-03-22

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Inventor after: Eric Joseph Sentenheimer

Inventor after: Shan Tianjian

Inventor after: Guitari Daifei

Inventor after: Zhang Han

Inventor after: Amir mill

Inventor after: Anastasia Kovorova

Inventor after: Jonathan Kenneth Worth

Inventor after: Nadia Amrani

Inventor after: Chen Zexiang

Inventor after: Hassler Matthew

Inventor after: Dimas Echeveria Moreno

Inventor after: Julia Francis Altman

Inventor after: Scott Wolf

Inventor before: Eric Joseph Sentenheimer

Inventor before: Shan Tianjian

Inventor before: Guitari Daifei

Inventor before: Zhang Han

Inventor before: Anastasia Kovorova

Inventor before: Jonathan Kenneth Worth

Inventor before: Nadia Amrani

Inventor before: Chen Zexiang

Inventor before: Hassler Matthew

Inventor before: Dimas Echeveria Moreno

Inventor before: Julia Francis Altman

Inventor before: Scott Wolf