CN115944699A - Traditional Chinese medicine composition with effects of activating spleen, tonifying qi, promoting digestion and removing food stagnation as well as preparation method and application thereof - Google Patents
Traditional Chinese medicine composition with effects of activating spleen, tonifying qi, promoting digestion and removing food stagnation as well as preparation method and application thereof Download PDFInfo
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Classifications
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Medicines Containing Plant Substances (AREA)
Abstract
The invention provides a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, which comprises the following raw materials in parts by weight: 6 to 14 parts of bran-fried bighead atractylodes rhizome, 6 to 14 parts of dried orange peel, 6 to 14 parts of fried chicken's gizzard-membrane, 6 to 14 parts of fried malt, 6 to 14 parts of fried medicated leaven, 4 to 10 parts of fried areca-nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg and 2 to 8 parts of poria cocos; 1-6 parts of coix seed, 1-6 parts of Chinese yam, 1-6 parts of green tangerine peel and 1-4 parts of honey-fried licorice root. The invention adopts the specific components and the proportion, has synergistic effect, has obvious treatment effect on diseases such as spleen dysfunction, dyspepsia and the like, and has obviously better drug effect than the stomach invigorating and digestion promoting tablets prepared by the traditional process.
Description
Technical Field
The invention relates to the technical field of traditional Chinese medicines, in particular to a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, and a preparation method and application thereof.
Background
With the improvement of living standard of people, the life rhythm is accelerated, and other factors, wherein diseases such as functional dyspepsia caused by weakness of spleen and stomach have obvious rising trend, and the life quality is influenced.
The existing stomach invigorating and digestion promoting tablet is prepared from radix Pseudostellariae, pericarpium Citri Tangerinae, rhizoma Dioscoreae, fructus Hordei Germinatus preparata, and fructus crataegi; pulverizing half amount of radix Pseudostellariae and rhizoma Dioscoreae powder into fine powder, decocting pericarpium Citri Tangerinae, rhizoma Dioscoreae, fructus Hordei Germinatus preparata, fructus crataegi and the rest radix Pseudostellariae with water for 2 hr twice, mixing decoctions, filtering, concentrating at low temperature to obtain soft extract, or concentrating to obtain fluid extract with relative density of 1.08-1.12 (65 deg.C). The extraction method is a traditional Chinese medicine preparation method, and has the advantages of laggard process, low content of effective components, slow action, and large dosage, and the dosage is 3-6 tablets per time and 3 times a day.
Therefore, it is very important to provide a tablet for invigorating stomach and promoting digestion, which has a drug effect obviously superior to that of the tablet prepared by the traditional process.
Disclosure of Invention
In view of the above, the traditional Chinese medicine composition provided by the invention has the effects of activating spleen and tonifying qi, and promoting digestion and resolving stagnation.
The invention provides a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, which comprises the following raw materials in parts by weight:
6 to 14 parts of bran-fried bighead atractylodes rhizome, 6 to 14 parts of dried orange peel, 6 to 14 parts of fried chicken's gizzard-membrane, 6 to 14 parts of fried malt, 6 to 14 parts of fried medicated leaven, 4 to 10 parts of fried areca nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg, 2 to 8 parts of poria cocos, 1 to 6 parts of coix seed, 1 to 6 parts of Chinese yam, 1 to 6 parts of green tangerine peel and 1 to 4 parts of honey-fried licorice root.
Preferably, the traditional Chinese medicine composition comprises the following raw materials in parts by weight:
6 to 13 portions of bran-fried largehead atractylodes rhizome, 6 to 13 portions of tangerine peel, 6 to 13 portions of fried chicken's gizzard-membrane, 6 to 13 portions of fried malt, 6 to 13 portions of fried medicated leaven, 4 to 10 portions of fried areca, 4 to 10 portions of fried hawthorn, 4 to 10 portions of radix pseudostellariae, 2 to 8 portions of amomum fruit, 2 to 8 portions of nutmeg, 2 to 8 portions of tuckahoe, 1 to 6 portions of coix seed, 1 to 6 portions of yam, 1 to 6 portions of green tangerine peel and 1 to 3 portions of honey-fried licorice root.
Preferably, the traditional Chinese medicine composition comprises the following raw materials in parts by weight:
6 to 12 parts of bran-fried bighead atractylodes rhizome, 6 to 12 parts of dried orange peel, 6 to 12 parts of fried chicken's gizzard-membrane, 6 to 12 parts of fried malt, 6 to 12 parts of fried medicated leaven, 4 to 10 parts of fried areca nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg, 2 to 8 parts of poria cocos, 1 to 6 parts of coix seed, 1 to 6 parts of Chinese yam, 1 to 6 parts of green tangerine peel and 1 to 2 parts of honey-fried licorice root.
The invention provides a preparation method of a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, which comprises the following steps:
a) Pulverizing pericarpium Citri Tangerinae, fructus Amomi, semen Myristicae, and pericarpium Citri Reticulatae viride, soaking in water, and extracting to obtain volatile oil and residue solution; dissolving the volatile oil with ethanol, and clathrating with cyclodextrin to obtain first extractive solution;
b) Crushing the fried hawthorn, the bran-fried bighead atractylodes rhizome, the poria cocos and the radix pseudostellariae, and extracting with 60-70% ethanol to obtain an ethanol extract; loading the alcohol extract on macroporous adsorption resin, eluting, and concentrating the eluent to obtain a second extract;
c) Reflux-extracting endothelium corneum Gigeriae Galli, coicis semen, fructus Hordei Germinatus preparata, rhizoma Dioscoreae, massa Medicata Fermentata preparata, arecae semen preparata, and radix Glycyrrhizae Preparata with the above residue solution, concentrating, mixing with ethanol, standing, and filtering to obtain primary extractive solution; putting the primary extract on resin, eluting and concentrating to obtain a third extract;
d) Mixing the first extractive solution, the second extractive solution and the third extractive solution, and drying.
Preferably, the crushed mesh number in the step A) is 40-50 meshes; the soaking time is 0.5-1 h; the material-liquid ratio is 1; the extraction is a steam distillation extraction method, and the extraction time is 6-8 h; the mass ratio of the cyclodextrin to the volatile oil is 6-10; the inclusion temperature is 45-55 ℃ and the inclusion time is 3-5 h.
Preferably, the extraction times in the step B) are 2-4 times; each time is 4-8 min; the mass concentration of the alcohol extract is 0.5g/mL; the macroporous adsorption resin is CAD-45 resin; the elution is specifically as follows: eluting with water of 4-6 times of column volume, discarding the eluent, and eluting with ethanol of 70% of 5 times of column volume; the concentration is reduced pressure concentration at 45-50 ℃.
Preferably, the reflux extraction frequency of the step C) is 1-2 times; the reflux time is 1 to 1.5 hours; the concentration is a thick paste with the relative density of 1.10-1.20; mixing with ethanol to obtain ethanol content of 60%; the standing time is 20-24 h.
Preferably, the mass concentration of the primary extract in the step C) is 0.5g/mL; the resin is CAD-45 resin; the elution is specifically that water with 4 times of column volume is adopted for elution, the elution liquid is discarded, and 55 percent of the column volume of 6 to 8 times is used for elution by ethanol; the concentration is reduced pressure concentration at 45-50 ℃.
The invention provides application of the composition or the composition prepared by the preparation method in preparing products for activating spleen and replenishing qi, and promoting digestion and resolving stagnation.
The invention provides a product for activating spleen, tonifying qi, promoting digestion and resolving stagnation, which comprises the composition or the composition prepared by the preparation method.
Compared with the prior art, the invention provides a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, which comprises the following raw materials in parts by weight: 6 to 14 parts of bran-fried bighead atractylodes rhizome, 6 to 14 parts of dried orange peel, 6 to 14 parts of fried chicken's gizzard-membrane, 6 to 14 parts of fried malt, 6 to 14 parts of fried medicated leaven, 4 to 10 parts of fried areca-nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg and 2 to 8 parts of poria cocos; 1-6 parts of coix seed, 1-6 parts of Chinese yam, 1-6 parts of green tangerine peel and 1-4 parts of honey-fried licorice root. The invention adopts the specific components and the proportion, has synergistic effect, has obvious treatment effect on diseases such as spleen dysfunction, dyspepsia and the like, and has obviously better drug effect than the stomach invigorating and digestion promoting tablets prepared by the traditional process.
Detailed Description
The invention provides a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, and a preparation method and application thereof. It is expressly intended that all such similar substitutes and modifications apparent to those skilled in the art are deemed to be within the scope of the invention. While the methods and applications of this invention have been described in terms of preferred embodiments, it will be apparent to those of ordinary skill in the art that variations and modifications in the methods and applications described herein, as well as other suitable variations and combinations, may be made to implement and use the techniques of this invention without departing from the spirit and scope of the invention.
The invention provides a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, which comprises the following raw materials in parts by weight:
6 to 14 parts of bran-fried bighead atractylodes rhizome, 6 to 14 parts of dried orange peel, 6 to 14 parts of fried chicken's gizzard-membrane, 6 to 14 parts of fried malt, 6 to 14 parts of fried medicated leaven, 4 to 10 parts of fried areca nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg, 2 to 8 parts of poria cocos, 1 to 6 parts of coix seed, 1 to 6 parts of Chinese yam, 1 to 6 parts of green tangerine peel and 1 to 4 parts of honey-fried licorice root.
The source of the traditional Chinese medicine is not limited and the traditional Chinese medicine can be sold in the market.
The traditional Chinese medicine composition provided by the invention comprises 6-14 parts by weight of bran-fried bighead atractylodes rhizome; preferably 6 to 13 parts by weight; more preferably 6 to 12 parts by weight.
The traditional Chinese medicine composition provided by the invention comprises 6-14 parts by weight of dried orange peel; preferably 6 to 13 parts by weight; more preferably 6 to 12 parts by weight.
The traditional Chinese medicine composition provided by the invention comprises 6-14 parts by weight of fried chicken's gizzard-membrane; preferably 6 to 13 parts by weight; more preferably from 6 to 12 parts by weight.
The traditional Chinese medicine composition provided by the invention comprises 6-14 parts by weight of roasted malt; preferably 6 to 13 parts by weight; more preferably from 6 to 12 parts by weight.
The traditional Chinese medicine composition provided by the invention comprises 6-14 parts by weight of fried medicated leaven; preferably 6 to 13 parts by weight; more preferably 6 to 12 parts by weight.
The traditional Chinese medicine composition provided by the invention comprises 4-10 parts by weight of fried areca; specifically, 4 parts by weight, 6 parts by weight, 8 parts by weight, or 10 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 4-10 parts by weight of fried hawthorn; specifically, it may be 4 parts by weight, 6 parts by weight, 8 parts by weight or 10 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 4-10 parts by weight of radix pseudostellariae; specifically, it may be 4 parts by weight, 6 parts by weight, 8 parts by weight or 10 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 2-8 parts by weight of fructus amomi; specifically, it may be 2 parts by weight, 4 parts by weight, 6 parts by weight or 8 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 2-8 parts by weight of nutmeg; specifically, 2 parts by weight, 4 parts by weight, 6 parts by weight or 8 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 2-8 parts by weight of poria cocos; specifically, it may be 2 parts by weight, 4 parts by weight, 6 parts by weight or 8 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 1-6 parts by weight of coix seeds; specifically, it may be 1 part by weight, 2 parts by weight, 4 parts by weight or 6 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 1-6 parts by weight of Chinese yam; specifically, it may be 1 part by weight, 2 parts by weight, 4 parts by weight or 6 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 1-6 parts by weight of pericarpium citri reticulatae viride; specifically, it may be 1 part by weight, 2 parts by weight, 4 parts by weight or 6 parts by weight; or a point value between any of the above.
The traditional Chinese medicine composition provided by the invention comprises 1-4 parts by weight of honey-fried licorice root; specifically, the amount of the compound is 1 part by weight, 2 parts by weight or 4 parts by weight; or a point value between any of the above.
In a part of preferred embodiments of the present invention, the Chinese medicinal composition comprises the following raw materials in parts by weight:
6 to 13 parts of bran-fried bighead atractylodes rhizome, 6 to 13 parts of dried orange peel, 6 to 13 parts of fried chicken's gizzard-membrane, 6 to 13 parts of fried malt, 6 to 13 parts of fried medicated leaven, 4 to 10 parts of fried areca-nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg and 2 to 8 parts of poria cocos; 1-6 parts of coix seed, 1-6 parts of Chinese yam, 1-6 parts of green tangerine peel and 1-3 parts of honey-fried licorice root.
In a part of preferred embodiments of the present invention, the Chinese medicinal composition comprises the following raw materials in parts by weight:
6 to 12 parts of bran-fried bighead atractylodes rhizome, 6 to 12 parts of dried orange peel, 6 to 12 parts of fried chicken's gizzard-membrane, 6 to 12 parts of fried malt, 6 to 12 parts of fried medicated leaven, 4 to 10 parts of fried areca-nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg and 2 to 8 parts of poria cocos; 1-6 parts of coix seed, 1-6 parts of Chinese yam, 1-6 parts of green tangerine peel and 1-2 parts of honey-fried licorice root.
In one preferred embodiment of the invention, the traditional Chinese medicine composition comprises the following raw materials in parts by weight: 12 parts of bran-fried bighead atractylodes rhizome, 12 parts of dried orange peel, 12 parts of fried chicken's gizzard-membrane, 12 parts of fried malt, 12 parts of fried medicated leaven, 10 parts of fried areca nut, 10 parts of fried hawthorn, 10 parts of radix pseudostellariae, 8 parts of fructus amomi, 8 parts of nutmeg and 8 parts of poria cocos; 6 parts of coix seeds, 6 parts of Chinese yam, 6 parts of green tangerine peels and 2 parts of honey-fried licorice roots;
in one preferred embodiment of the present invention, the Chinese medicinal composition comprises the following raw materials in parts by weight: 10 parts of bran-fried bighead atractylodes rhizome, 10 parts of dried orange peel, 10 parts of fried chicken's gizzard-membrane, 10 parts of fried malt, 10 parts of fried medicated leaven, 8 parts of fried areca nut, 8 parts of fried hawthorn, 8 parts of radix pseudostellariae, 6 parts of fructus amomi, 6 parts of nutmeg and 6 parts of poria cocos; 4 parts of coix seeds, 4 parts of Chinese yam, 4 parts of green tangerine peels and 2 parts of honey-fried licorice roots;
in one preferred embodiment of the present invention, the Chinese medicinal composition comprises the following raw materials in parts by weight: 10 parts of bran-fried bighead atractylodes rhizome, 10 parts of dried orange peel, 12 parts of fried chicken's gizzard-membrane, 12 parts of fried malt, 8 parts of fried medicated leaven, 8 parts of fried areca seed, 6 parts of fried hawthorn, 6 parts of radix pseudostellariae, 6 parts of fructus amomi, 6 parts of nutmeg and 6 parts of poria cocos; 4 parts of coix seeds, 4 parts of Chinese yam, 4 parts of green tangerine peels and 2 parts of honey-fried licorice roots;
in one preferred embodiment of the present invention, the Chinese medicinal composition comprises the following raw materials in parts by weight: 8 parts of bran-fried bighead atractylodes rhizome, 8 parts of dried orange peel, 8 parts of fried chicken's gizzard-membrane, 8 parts of fried malt, 8 parts of fried medicated leaven, 6 parts of fried areca nut, 6 parts of fried hawthorn, 6 parts of radix pseudostellariae, 4 parts of fructus amomi, 4 parts of nutmeg and 4 parts of poria cocos; 2 parts of coix seeds, 2 parts of Chinese yam, 2 parts of green tangerine orange peel and 2 parts of honey-fried licorice roots;
in one preferred embodiment of the present invention, the Chinese medicinal composition comprises the following raw materials in parts by weight: 6 parts of bran-fried bighead atractylodes rhizome, 6 parts of dried orange peel, 6 parts of fried chicken's gizzard-membrane, 6 parts of fried malt, 6 parts of fried medicated leaven, 4 parts of fried areca nut, 4 parts of fried hawthorn, 4 parts of radix pseudostellariae, 2 parts of fructus amomi, 2 parts of nutmeg and 2 parts of poria cocos; 1 part of coix seed, 1 part of Chinese yam, 1 part of green tangerine peel and 1 part of honey-fried licorice root.
The invention adds the medicated leaven and the betel nut which are fried to be four immortals, adds the atractylodes macrocephala, the tuckahoe, the coix seed and the fried chicken gizzard membrane to strengthen the efficacy of strengthening the spleen and stomach, adds the amomum fruit, the nutmeg and the green tangerine peel which are good at strengthening the spleen and regulating the flow of qi, eliminating the food retention and relieving the stagnation, and adds the honey-fried licorice root which relieves spasm and pain to harmonize the medicines, and simultaneously carries out enrichment, separation and purification of effective parts of all the medicinal materials.
The invention provides a preparation method of a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, which comprises the following steps:
a) Pulverizing pericarpium Citri Tangerinae, fructus Amomi, semen Myristicae, and pericarpium Citri Reticulatae viride, soaking in water, and extracting to obtain volatile oil and residue solution; dissolving the volatile oil with ethanol, and clathrating with cyclodextrin to obtain first extractive solution;
b) Crushing the fried hawthorn, the bran-fried bighead atractylodes rhizome, the poria cocos and the radix pseudostellariae, and extracting with 60-70% ethanol to obtain an ethanol extract; loading the alcohol extract on macroporous adsorption resin, eluting, and concentrating the eluent to obtain a second extract;
c) Reflux-extracting endothelium corneum Gigeriae Galli, coicis semen, fructus Hordei Germinatus preparata, rhizoma Dioscoreae, massa Medicata Fermentata preparata, arecae semen preparata, and radix Glycyrrhizae Preparata with the above residue solution, concentrating, mixing with ethanol, standing, and filtering to obtain primary extractive solution; putting the primary extract on resin, eluting and concentrating to obtain a third extract;
d) Mixing the first extractive solution, the second extractive solution and the third extractive solution, and drying.
The preparation method of the traditional Chinese medicine composition with the effects of activating spleen, tonifying qi, promoting digestion and resolving stagnation, provided by the invention, comprises the steps of crushing pericarpium citri reticulatae, fructus amomi, nutmeg and pericarpium citri reticulatae viride.
The crushed mesh number is 40-50 meshes; more preferably 40 mesh. The material-liquid ratio is 1; namely, the mass ratio of the dried orange peel, the amomum fruit, the nutmeg, the green tangerine peel and the water is 1; the soaking time is 0.5-1 h; the extraction is a steam distillation extraction method, volatile oil is extracted by steam distillation for 6 to 8 hours, the volatile oil is collected and dissolved by equal amount of ethanol for later use.
Weighing cyclodextrin with 6-10 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3-5 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin clathrate compound.
Crushing the fried hawthorn, the bran-fried bighead atractylodes rhizome, the poria cocos and the radix pseudostellariae into coarse powder, carrying out flash extraction with 60-70% of ethanol for 4-8 minutes each time for 2-4 times, filtering the extracted medicine residues, and recovering the ethanol at the temperature below 50 ℃. And adding water to dilute the obtained extracting solution to 0.5g of crude drug per milliliter, namely the mass concentration of the alcohol extracting solution is 0.5g/mL.
Passing through the treated macroporous adsorption resin, wherein the macroporous adsorption resin is CAD-45; the elution is specifically as follows: eluting with water of 4-6 times of column volume, discarding the eluent, and eluting with ethanol of 70% of 5 times of column volume; the concentration is reduced pressure concentration at 45-50 ℃.
Reflux-extracting endothelium corneum Gigeriae Galli preparata, coicis semen, fructus Hordei Germinatus preparata, rhizoma Dioscoreae, massa Medicata Fermentata preparata, arecae semen preparata, and radix Glycyrrhizae Preparata with the above residue solution. The medicine residue liquid is the medicine residue liquid obtained in the step A). The reflux extraction times are 1-2; the reflux time is 1 to 1.5 hours; more preferably, the reflux extraction is performed 1 to 2 times; the reflux time is 1.1 to 1.4 hours; filtering, concentrating the filtrate to obtain soft extract with relative density of 1.10-1.20 deg.C, adding 95% ethanol to make ethanol content reach 60%, and standing; the standing time is 20-24 h; more preferably 24h.
Filtering, and recovering ethanol from the filtrate. Diluting the obtained extract with water to 0.5g crude drug per ml, which is: the mass concentration of the primary extract is 0.5g/mL; through the treated CAD-45 resin; the elution is specifically that water with 4 times of column volume is adopted for elution, the elution liquid is discarded, and 55 percent of the column volume of 6 to 8 times is used for elution by ethanol; the concentration is reduced pressure concentration at 45-50 ℃.
Mixing the first extractive solution, the second extractive solution and the third extractive solution, and drying. The mixing method of the present invention is not limited, and those skilled in the art will be familiar with it. The present invention is not limited to the specific manner of drying, and those skilled in the art will be familiar with the drying method.
The invention adopts the technical means of steam distillation extraction, ethanol flash extraction, ethanol heating reflux extraction, macroporous adsorption resin and the like to enrich, separate and purify the effective parts with different medicinal flavors, and the method can furthest extract the active ingredients such as hesperidin, adenosine and the like in the raw materials. The preparation method of the invention is adopted to enrich, separate and purify the effective part, and experiments show that the traditional Chinese medicine composition has obvious treatment effect on diseases such as functional dyspepsia caused by weakness of the spleen and the stomach.
The invention provides application of the composition or the composition prepared by the preparation method in preparing products for activating spleen and replenishing qi, and promoting digestion and resolving stagnation.
The invention provides a product for activating spleen, tonifying qi, promoting digestion and resolving stagnation, which comprises the composition or the composition prepared by the preparation method.
The product of the invention includes but is not limited to food, medicine or health care product. Also comprises auxiliary materials or ingredients which are commonly used in the field of medicines or foods.
Pharmacodynamic experiments prove that the traditional Chinese medicine composition prepared by the invention has obvious efficacy, high bioavailability and stable and reliable quality, can be prepared into different dosage forms according to the requirements of patients, and is convenient to carry and easy to take.
The dosage form of the invention includes but is not limited to tablets, powders, granules, pills and the like.
The invention provides a traditional Chinese medicine composition with the effects of activating spleen, tonifying qi, promoting digestion and resolving stagnation, which comprises the following raw materials in parts by weight: 6 to 14 parts of bran-fried bighead atractylodes rhizome, 6 to 14 parts of dried orange peel, 6 to 14 parts of fried chicken's gizzard-membrane, 6 to 14 parts of fried malt, 6 to 14 parts of fried medicated leaven, 4 to 10 parts of fried areca-nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg and 2 to 8 parts of poria cocos; 1-6 parts of coix seeds, 1-6 parts of Chinese yam, 1-6 parts of green tangerine orange peel and 1-4 parts of honey-fried licorice roots. The invention adopts the specific components and the proportion, has synergistic effect, has obvious treatment effect on diseases such as spleen dysfunction, dyspepsia and the like, and has obviously better drug effect than the stomach invigorating and digestion promoting tablets prepared by the traditional process.
In order to further explain the invention, the following embodiments are combined to describe the traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation, and the preparation method and the application thereof in detail.
Example 1
The formula is as follows: 12 parts of bran-fried bighead atractylodes rhizome, 12 parts of dried orange peel, 12 parts of fried chicken's gizzard-membrane, 12 parts of fried malt, 12 parts of fried medicated leaven, 10 parts of fried areca nut, 10 parts of fried hawthorn, 10 parts of radix pseudostellariae, 8 parts of fructus amomi, 8 parts of nutmeg and 8 parts of poria cocos; 6 parts of coix seed, 6 parts of Chinese yam, 6 parts of green tangerine peel and 2 parts of honey-fried licorice root
(1) Pulverizing pericarpium Citri Tangerinae, fructus Amomi, semen Myristicae, and pericarpium Citri Reticulatae viride into fine powder, sieving with 40 mesh sieve, adding 10 times of water, soaking for 1 hr, extracting volatile oil by steam distillation for 6 hr, collecting volatile oil, and dissolving with equal amount of ethanol; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin volatile oil.
(2) Pulverizing parched fructus crataegi, atractylodis rhizoma, poria and radix Pseudostellariae into coarse powder, extracting with 70% ethanol under flash extraction for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
(3) Taking fried chicken's gizzard-membrane, coix seed, fried malt, chinese yam, fried medicated leaven, fried areca nut and honey-fried licorice root, extracting the dregs and the extracting solution in the step (1) for 2 times by hot reflux, each time for 1 hour, filtering, concentrating the filtrate into thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the alcohol content reaches 60%, standing for 24 hours, filtering, and recovering the ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% of 6-8 times column volume of ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Example 2
The formula is as follows: 10 parts of bran-fried bighead atractylodes rhizome, 10 parts of dried orange peel, 10 parts of fried chicken's gizzard-membrane, 10 parts of fried malt, 10 parts of fried medicated leaven, 8 parts of fried areca nut, 8 parts of fried hawthorn, 8 parts of radix pseudostellariae, 6 parts of fructus amomi, 6 parts of nutmeg and 6 parts of poria cocos; coix seed 4 parts, chinese yam 4 parts, green tangerine orange peel 4 parts, honey-fried licorice root 2 parts
Pulverizing pericarpium citri reticulatae, fructus amomi, nutmeg and pericarpium citri reticulatae viride into fine powder, sieving the fine powder by a 40-mesh sieve, adding 10 times of water, soaking for 1 hour, extracting volatile oil for 6 hours by steam distillation, collecting the volatile oil, and dissolving the volatile oil by using equivalent ethanol for later use; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin clathrate compound.
Pulverizing parched fructus crataegi, atractylodis rhizoma, poria and radix Pseudostellariae into coarse powder, extracting with 70% ethanol by flash extraction for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (3) taking fried chicken gizzard membrane, coix seed, fried malt, chinese yam, fried medicated leaven, fried betel nut and honey-fried licorice root, extracting the dregs and the extracting solution in the step (1) by hot reflux for 2 times, wherein each time is 1 hour, filtering, concentrating the filtrate into thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the alcohol content reaches 60%, standing for 24 hours, filtering, and recovering the ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% of 6-8 times column volume of ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Example 3
The formula is as follows: 10 parts of bran-fried bighead atractylodes rhizome, 10 parts of dried orange peel, 12 parts of fried chicken's gizzard-membrane, 12 parts of fried malt, 8 parts of fried medicated leaven, 8 parts of fried areca nut, 6 parts of fried hawthorn, 6 parts of radix pseudostellariae, 6 parts of fructus amomi, 6 parts of nutmeg and 6 parts of poria cocos; coix seed 4 parts, chinese yam 4 parts, green tangerine orange peel 4 parts, honey-fried licorice root 2 parts
Pulverizing pericarpium citri reticulatae, fructus amomi, nutmeg and pericarpium citri reticulatae viride into fine powder, sieving the fine powder with a 40-mesh sieve, adding 10 times of water, soaking for 1 hour, extracting volatile oil for 6 hours by steam distillation, collecting the volatile oil, and dissolving the volatile oil with equal amount of ethanol for later use; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin volatile oil.
Pulverizing parched fructus crataegi, atractylodis rhizoma, poria and radix Pseudostellariae into coarse powder, extracting with 70% ethanol by flash extraction for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (3) taking fried chicken gizzard membrane, coix seed, fried malt, chinese yam, fried medicated leaven, fried betel nut and honey-fried licorice root, extracting the dregs and the extracting solution in the step (1) by hot reflux for 2 times, wherein each time is 1 hour, filtering, concentrating the filtrate into thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the alcohol content reaches 60%, standing for 24 hours, filtering, and recovering the ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% of 6-8 times column volume of ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Example 4
The formula is as follows: 8 parts of bran-fried bighead atractylodes rhizome, 8 parts of dried orange peel, 8 parts of fried chicken's gizzard-membrane, 8 parts of fried malt, 8 parts of fried medicated leaven, 6 parts of fried areca nut, 6 parts of fried hawthorn, 6 parts of radix pseudostellariae, 4 parts of fructus amomi, 4 parts of nutmeg and 4 parts of poria cocos; 2 parts of coix seed, 2 parts of Chinese yam, 2 parts of green tangerine peel and 2 parts of honey-fried licorice root
Pulverizing pericarpium citri reticulatae, fructus amomi, nutmeg and pericarpium citri reticulatae viride into fine powder, sieving the fine powder with a 40-mesh sieve, adding 10 times of water, soaking for 1 hour, extracting volatile oil for 6 hours by steam distillation, collecting the volatile oil, and dissolving the volatile oil with equal amount of ethanol for later use; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin volatile oil.
Pulverizing parched fructus crataegi, atractylodis rhizoma, poria and radix Pseudostellariae into coarse powder, extracting with 70% ethanol by flash extraction for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (3) taking fried chicken gizzard membrane, coix seed, fried malt, chinese yam, fried medicated leaven, fried betel nut, dregs of a decoction and an extracting solution in the step (1), extracting for 2 times by hot reflux, filtering for 1 hour each time, concentrating the filtrate to thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the ethanol content reaches 60%, standing for 24 hours, filtering, and recovering ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% of 6-8 times column volume of ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Example 5
The formula is as follows: 6 parts of bran-fried bighead atractylodes rhizome, 6 parts of dried orange peel, 6 parts of fried chicken's gizzard-membrane, 6 parts of fried malt, 6 parts of fried medicated leaven, 4 parts of fried areca nut, 4 parts of fried hawthorn, 4 parts of radix pseudostellariae, 2 parts of fructus amomi, 2 parts of nutmeg and 2 parts of poria cocos; 1 part of coix seed, 1 part of Chinese yam, 1 part of green tangerine peel and 1 part of honey-fried licorice root
Pulverizing pericarpium citri reticulatae, fructus amomi, nutmeg and pericarpium citri reticulatae viride into fine powder, sieving the fine powder by a 40-mesh sieve, adding 10 times of water, soaking for 1 hour, extracting volatile oil for 6 hours by steam distillation, collecting the volatile oil, and dissolving the volatile oil by using equivalent ethanol for later use; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin volatile oil.
Pulverizing parched fructus crataegi, atractylodis rhizoma, poria and radix Pseudostellariae into coarse powder, extracting with 70% ethanol by flash extraction for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (3) taking fried chicken gizzard membrane, coix seed, fried malt, chinese yam, fried medicated leaven, fried betel nut and honey-fried licorice root, extracting the dregs and the extracting solution in the step (1) by hot reflux for 2 times, wherein each time is 1 hour, filtering, concentrating the filtrate into thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the alcohol content reaches 60%, standing for 24 hours, filtering, and recovering the ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% ethanol 6-8 times column volume, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Comparative example 1
The formula is as follows: 12 parts of radix astragali preparata, 12 parts of pinellia ternate, 12 parts of fried chicken's gizzard-membrane, 12 parts of fried rice sprout, 12 parts of fried medicated leaven, 10 parts of fried areca nut, 10 parts of fried hawthorn, 10 parts of radix pseudostellariae, 8 parts of fructus amomi, 8 parts of nutmeg and 8 parts of poria cocos; 6 parts of coix seed, 6 parts of Chinese yam, 6 parts of green tangerine peel and 2 parts of honey-fried licorice root
Pulverizing fructus amomi, nutmeg and pericarpium citri reticulatae viride into fine powder, sieving the fine powder with a 40-mesh sieve, adding 10 times of water, soaking for 1 hour, extracting volatile oil for 6 hours by steam distillation, collecting the volatile oil, and dissolving the volatile oil with equal amount of ethanol for later use; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin clathrate compound.
Pulverizing parched fructus crataegi, rhizoma Pinelliae, radix astragali Preparata, poria and radix Pseudostellariae into coarse powder, extracting with 70% ethanol by flash extraction for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (3) taking fried chicken's gizzard-membrane, coix seed, fried rice sprout, chinese yam, fried medicated leaven, fried areca seed and honey-fried licorice root, extracting the dregs and the extracting solution in the step (1) by hot reflux for 2 times, 1 hour each time, filtering, concentrating the filtrate to thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the alcohol content reaches 60%, standing for 24 hours, filtering, and recovering the ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% of 6-8 times column volume of ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Comparative example 2
The formula is as follows: 12 parts of fried chicken's gizzard-membrane, 12 parts of fried malt, 12 parts of fried medicated leaven, 10 parts of fried betel nut, 10 parts of fried hawthorn, 8 parts of fructus amomi, 8 parts of nutmeg and 8 parts of poria cocos; 6 parts of coix seed, 6 parts of Chinese yam, 6 parts of green tangerine peel and 2 parts of honey-fried licorice root
(1) Pulverizing fructus Amomi, semen Myristicae, and pericarpium Citri Reticulatae viride into fine powder, sieving with 40 mesh sieve, adding 10 times of water, soaking for 1 hr, extracting volatile oil by steam distillation for 6 hr, collecting volatile oil, and dissolving with equal amount of ethanol; weighing cyclodextrin with 8 times of volatile oil amount to prepare a saturated aqueous solution at 45-55 ℃, adding the volatile oil while stirring, continuously stirring for 3 hours at the temperature of 45-55 ℃, refrigerating overnight, filtering, and airing to obtain the cyclodextrin volatile oil.
(2) Pulverizing parched fructus crataegi and Poria into coarse powder, extracting with 70% ethanol for 4 min for 2-4 times, filtering the residue, and recovering ethanol at 50 deg.C or below. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through treated macroporous adsorbent resin, eluting with 4 times column volume of water, discarding eluate, eluting with 5 times column volume of 70% ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
(3) Taking fried chicken's gizzard-membrane, coix seed, fried malt, chinese yam, fried medicated leaven, fried areca nut and honey-fried licorice root, extracting the dregs and the extracting solution in the step (1) for 2 times by hot reflux, each time for 1 hour, filtering, concentrating the filtrate into thick paste with the relative density of 1.10-1.20 ℃, adding 95% ethanol to ensure that the alcohol content reaches 60%, standing for 24 hours, filtering, and recovering the ethanol from the filtrate. Diluting the obtained extractive solution with water to 0.5g crude drug per ml, passing through processed CAD-45 resin, eluting with 4 times column volume of water, discarding eluate, eluting with 55% of 6-8 times column volume of ethanol, collecting ethanol eluate, and concentrating under reduced pressure below 50 deg.C to obtain the final product.
And (4) uniformly mixing the extracting solutions obtained in the step (1), the step (2) and the step (3) and drying to obtain the compound.
Pharmacodynamic study of proof examples
1. Experimental Material
1.1 Test drugs
The tablet for invigorating stomach and promoting digestion, the original tablet for invigorating stomach and promoting digestion, the deficient prescription and the alternative preparation are provided by Jilin revised pharmaceutical industry New drug development Limited company.
1.2 animals
Wistar rats, 110, male and female halves; the number of ICR mice is 110, half of male and female, the ICR mice are in SPF grade, the animal production license number is SCXK (Ji) -2020-0002, and the high-protein high-heat feed and the SPF big and small mouse maintenance feed are provided by Yinss experimental animal technology Limited liability company in Changchun city.
1.3 reagent
Gastrin kit, motilin kit and cholinesterase kit were purchased from Nanjing Biotechnology Ltd.
2 method of experiment
2.1 Effect on secretion of hormones (gastrin, motilin) and neurotransmitters (cholinesterase) from the gastrointestinal tract of rats in the spleen deficiency and indigestion model
2.1.1 Experimental methods
After the animals are bought, the animals are fed with water and food freely for 3d, the animals are randomly divided into 11 groups according to the body weight, each group comprises 10 animals, and the groups respectively comprise a blank control group, a model group, a CN200710017728.5 preparation group, an original stomach invigorating and digestion promoting tablet group, a prescription lack group, a replacement group, an example 1, an example 2, an example 3, an example 4 and an example 5, and the body weight, the abdominal circumference, and the average food intake and the excrement intake of each mouse in the current day of the animals in each group before the model building are recorded. The blank control group was normally bred, and 2ml/100g of distilled water was intragastrically administered. The model group rats are fed with self-made high-protein and high-heat feed (the feed is prepared by mixing dried fish floss, bean flour, flour and milk powder according to the proportion of 1. The corresponding liquid medicine 2ml/100g is respectively filled in each group of administration, equal volume of distilled water is given to the model group and the normal control group once a day for 7 days continuously, and the body mass, the abdominal circumference, the average food intake and the excrement amount of each mouse in the current day are recorded after treatment. Before the last administration, rats in each group are fasted for 24 hours, after the last administration or the distilled water filling, blood is taken from orbital veins, 3000r/min and centrifuged for 15min, serum is separated, an ELISA kit is used for measuring the levels of gastrin, motilin and cholinesterase in the serum, and the specific operation is carried out according to the instructions in the kit.
2.1.2 results of the experiment
The levels of gastrin, motilin and cholinesterase were significantly reduced in the model group compared to the blank group (P < 0.001). Compared with a model group, the levels of gastrin, motilin and cholinesterase of each administration group are remarkably improved (P < 0.001), wherein the effects of the examples 1 to 5 are better than those of the original stomach invigorating and digestion promoting tablet, the CN200710017728.5 preparation, the deficient prescription and the alternative preparation. See table 1 for details.
TABLE 1 influence of Gastrin and cholinesterase in serum of rats with spleen deficiency and indigestion model: (
n＝10)/>
Note: in comparison with the blank set, the results, △△△ P<0.001; in comparison with the set of models, ### P<0.001; compared with the original tablet group for invigorating stomach and promoting digestion, * P<0.05， ** P<0.01， *** P<0.001; compared with the CN200710017728.5 preparation group, ▲ P<0.05， ▲▲ P<0.01， ▲▲▲ P<0.001。
2.2 Effect on intestinal propulsion of spleen deficiency dyspepsia model mice
2.2.1 Experimental methods
After the animals are purchased, the animals are fed with free diet and water for 3 days, the animals are randomly divided into 11 groups according to the body weight, and each group comprises 10 animals, namely a blank control group, a model group, a CN200710017728.5 preparation group, an original stomach invigorating and digestion promoting tablet group, a lacking formula group, an alternative group, an embodiment 1, an embodiment 2, an embodiment 3, an embodiment 4 and an embodiment 5. The blank control group is normally raised, distilled water is filled into the stomach for 0.2ml/10g, the other groups of mice are fed with self-made high-protein and high-heat feed every day (the feed is prepared from dried fish floss, bean flour, flour and milk powder according to the proportion of 1. The administration of each group is respectively filled with corresponding liquid medicine of 0.2ml/10g, the model group and the normal control group are administered with distilled water of equal volume once a day for 7 days, and each group of mice fasted for 18 hours before the last administration are not forbidden to supply water. After the last administration or 1h of distilled water, each group of mice was gavaged with 0.2ml/10g of freshly prepared 5% charcoal powder suspension. After 10min, the small intestine was quickly removed, the mesentery was gently peeled off and laid flat, and the distance between the full length of the small intestine and the distance that the carbon powder suspension pushed from the pyloric sphincter to the end of the small intestine was measured. The small intestine propulsion rate was calculated as "(distance from pylorus to front of black charcoal powder suspension/full length of small intestine) × 100%)".
2.2.2 results of the experiment
Compared with the blank group, the small intestine propulsion rate of the model group is extremely obviously reduced (P < 0.001), which indicates that the gastrointestinal motility of the model group is inhibited. Compared with the model group, the small intestine propulsion rate (P <0.01, P < -0.001) of the spleen deficiency food accumulation model mouse can be obviously improved by each administration group, wherein the effects of the examples 1 to 5 are better than the effects of the original stomach invigorating and food stagnation eliminating tablet, the CN200710017728.5 preparation, the lack formula and the replacement preparation. See table 2 for details.
TABLE 2 Effect on intestinal propulsion in spleen deficiency dyspepsia model mice: (
n＝10)/>
Note: in comparison to the blank set, the results, △△△ P<0.001; in comparison with the set of models, ## P<0.01， ### P<0.001; compared with the original tablet group for invigorating stomach and promoting digestion, *** P<0.001; compared with the CN200710017728.5 preparation group, ▲ P<0.05， ▲▲ P<0.01， ▲▲▲ P<0.001。
3 conclusion
Through the pharmacodynamic experiments carried out on the traditional Chinese medicine composition, the traditional Chinese medicine composition disclosed by the invention can improve the small intestine propulsion rate of a spleen deficiency indigestion model animal, promote the secretion of gastrin and motilin, increase the level of cholinesterase, promote the gastrointestinal peristalsis through the dual regulation of gastrointestinal tract hormone and neurotransmitter, and enable the motor function of the digestive tract of an organism to tend to be normal. The effect is obviously superior to the original stomach invigorating and digestion promoting tablet, CN200710017728.5 preparation, absent prescription and alternative preparation.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and amendments can be made without departing from the principle of the present invention, and these modifications and amendments should also be considered as the protection scope of the present invention.
Claims (10)
1. A traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation is characterized by comprising the following raw materials in parts by weight:
6 to 14 parts of bran-fried bighead atractylodes rhizome, 6 to 14 parts of dried orange peel, 6 to 14 parts of fried chicken's gizzard-membrane, 6 to 14 parts of fried malt, 6 to 14 parts of fried medicated leaven, 4 to 10 parts of fried areca nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg, 2 to 8 parts of poria cocos, 1 to 6 parts of coix seed, 1 to 6 parts of Chinese yam, 1 to 6 parts of green tangerine peel and 1 to 4 parts of honey-fried licorice root.
2. The composition of claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight:
6 to 13 portions of bran-fried largehead atractylodes rhizome, 6 to 13 portions of tangerine peel, 6 to 13 portions of fried chicken's gizzard-membrane, 6 to 13 portions of fried malt, 6 to 13 portions of fried medicated leaven, 4 to 10 portions of fried areca-nut, 4 to 10 portions of fried hawthorn, 4 to 10 portions of radix pseudostellariae, 2 to 8 portions of fructus amomi, 2 to 8 portions of nutmeg, 2 to 8 portions of tuckahoe, 1 to 6 portions of coix seed, 1 to 6 portions of yam, 1 to 6 portions of green tangerine peel and 1 to 3 portions of honey-fried licorice root.
3. The composition of claim 1, wherein the traditional Chinese medicine composition comprises the following raw materials in parts by weight:
6 to 12 parts of bran-fried bighead atractylodes rhizome, 6 to 12 parts of dried orange peel, 6 to 12 parts of fried chicken's gizzard-membrane, 6 to 12 parts of fried malt, 6 to 12 parts of fried medicated leaven, 4 to 10 parts of fried areca nut, 4 to 10 parts of fried hawthorn, 4 to 10 parts of radix pseudostellariae, 2 to 8 parts of fructus amomi, 2 to 8 parts of nutmeg, 2 to 8 parts of poria cocos, 1 to 6 parts of coix seed, 1 to 6 parts of Chinese yam, 1 to 6 parts of green tangerine peel and 1 to 2 parts of honey-fried licorice root.
4. A preparation method of a traditional Chinese medicine composition with the effects of activating spleen and replenishing qi, and promoting digestion and resolving stagnation is characterized by comprising the following steps:
a) Pulverizing pericarpium Citri Tangerinae, fructus Amomi, semen Myristicae, and pericarpium Citri Reticulatae viride, soaking in water, and extracting to obtain volatile oil and residue solution; dissolving the volatile oil with ethanol, and clathrating with cyclodextrin to obtain first extractive solution;
b) Crushing fried hawthorn, bran-fried bighead atractylodes rhizome, poria cocos and radix pseudostellariae, and extracting with 60-70% ethanol to obtain an ethanol extract; loading the alcohol extract on macroporous adsorption resin, eluting, and concentrating the eluent to obtain a second extract;
c) Reflux-extracting endothelium corneum Gigeriae Galli, coicis semen, fructus Hordei Germinatus preparata, rhizoma Dioscoreae, massa Medicata Fermentata preparata, arecae semen preparata, and radix Glycyrrhizae Preparata with the above residue solution, concentrating, mixing with ethanol, standing, and filtering to obtain primary extractive solution; putting the primary extract on resin, eluting and concentrating to obtain a third extract;
d) Mixing the first extractive solution, the second extractive solution and the third extractive solution, and drying.
5. The method according to claim 4, wherein the crushed particles of step A) have a mesh size of 40 to 50 mesh; the soaking time is 0.5-1 h; the material-liquid ratio is 1; the extraction is a steam distillation extraction method, and the extraction time is 6-8 h; the mass ratio of the cyclodextrin to the volatile oil is 6-10; the inclusion temperature is 45-55 ℃, and the inclusion time is 3-5 h.
6. The method according to claim 4, wherein the number of times of extraction in the step B) is 2 to 4; each time is 4-8 min; the mass concentration of the alcohol extract is 0.5g/mL; the macroporous adsorption resin is CAD-45 resin; the elution is specifically as follows: eluting with water of 4-6 times of column volume, discarding the eluent, and eluting with ethanol of 70% of 5 times of column volume; the concentration is reduced pressure concentration at 45-50 ℃.
7. The method according to claim 4, wherein the reflux extraction of step C) is performed 1 to 2 times; the reflux time is 1 to 1.5 hours; the concentration is a thick paste with the relative density of 1.10-1.20; mixing with ethanol to obtain 60% ethanol content; the standing time is 20-24 h.
8. The preparation method according to claim 4, wherein the mass concentration of the primary extract in the step C) is 0.5g/mL; the resin is CAD-45 resin; the elution is specifically to elute by adopting water with 4 times of column volume, discard the eluent and elute by using 55 percent of 6 to 8 times of column volume with ethanol; the concentration is reduced pressure concentration at 45-50 ℃.
9. Use of the composition according to any one of claims 1 to 3 or the composition prepared by the preparation method according to any one of claims 4 to 8 in the preparation of a product for activating spleen and replenishing qi, and promoting digestion and resolving stagnation.
10. A product for activating the spleen and replenishing qi, and promoting digestion and resolving food stagnation, which comprises the composition of any one of claims 1 to 3 or the composition prepared by the preparation method of any one of claims 4 to 8.
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