CN115943963A - Sterilization composition containing methionin and dichlobeniazox and application thereof - Google Patents
Sterilization composition containing methionin and dichlobeniazox and application thereof Download PDFInfo
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- 239000000203 mixture Substances 0.000 title claims abstract description 38
- 230000001954 sterilising effect Effects 0.000 title claims description 5
- XUYPXLNMDZIRQH-LURJTMIESA-N N-acetyl-L-methionine Chemical compound CSCC[C@@H](C(O)=O)NC(C)=O XUYPXLNMDZIRQH-LURJTMIESA-N 0.000 title description 3
- 229930182817 methionine Natural products 0.000 title description 3
- 238000004659 sterilization and disinfection Methods 0.000 title description 3
- 230000000844 anti-bacterial effect Effects 0.000 claims abstract description 22
- 201000010099 disease Diseases 0.000 claims abstract description 21
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 claims abstract description 21
- 239000003795 chemical substances by application Substances 0.000 claims description 25
- 235000007164 Oryza sativa Nutrition 0.000 claims description 17
- 235000009566 rice Nutrition 0.000 claims description 17
- 240000008067 Cucumis sativus Species 0.000 claims description 15
- 235000010799 Cucumis sativus var sativus Nutrition 0.000 claims description 15
- 125000001434 methanylylidene group Chemical group [H]C#[*] 0.000 claims description 11
- 239000000375 suspending agent Substances 0.000 claims description 8
- 239000004480 active ingredient Substances 0.000 claims description 7
- 239000002270 dispersing agent Substances 0.000 claims description 6
- 239000003995 emulsifying agent Substances 0.000 claims description 6
- 239000002904 solvent Substances 0.000 claims description 6
- 239000000080 wetting agent Substances 0.000 claims description 6
- 206010039509 Scab Diseases 0.000 claims description 5
- 239000006184 cosolvent Substances 0.000 claims description 5
- 239000000945 filler Substances 0.000 claims description 5
- 241000233679 Peronosporaceae Species 0.000 claims description 4
- 239000012752 auxiliary agent Substances 0.000 claims description 4
- 239000000839 emulsion Substances 0.000 claims description 4
- 239000004530 micro-emulsion Substances 0.000 claims description 4
- 239000004308 thiabendazole Substances 0.000 claims description 4
- 235000010296 thiabendazole Nutrition 0.000 claims description 4
- 229960004546 thiabendazole Drugs 0.000 claims description 4
- 239000002562 thickening agent Substances 0.000 claims description 4
- 239000004562 water dispersible granule Substances 0.000 claims description 4
- 239000004563 wettable powder Substances 0.000 claims description 4
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 claims description 3
- 229920000742 Cotton Polymers 0.000 claims description 3
- 241000223218 Fusarium Species 0.000 claims description 3
- 240000008790 Musa x paradisiaca Species 0.000 claims description 3
- 235000018290 Musa x paradisiaca Nutrition 0.000 claims description 3
- 241000233622 Phytophthora infestans Species 0.000 claims description 3
- 241000221696 Sclerotinia sclerotiorum Species 0.000 claims description 3
- 235000021307 Triticum Nutrition 0.000 claims description 3
- 241000082085 Verticillium <Phyllachorales> Species 0.000 claims description 3
- 239000002518 antifoaming agent Substances 0.000 claims description 3
- 241000723873 Tobacco mosaic virus Species 0.000 claims description 2
- 230000000855 fungicidal effect Effects 0.000 claims description 2
- 239000008187 granular material Substances 0.000 claims description 2
- 239000003755 preservative agent Substances 0.000 claims description 2
- 230000002335 preservative effect Effects 0.000 claims description 2
- 240000007594 Oryza sativa Species 0.000 claims 5
- WJCNZQLZVWNLKY-UHFFFAOYSA-N thiabendazole Chemical compound S1C=NC(C=2NC3=CC=CC=C3N=2)=C1 WJCNZQLZVWNLKY-UHFFFAOYSA-N 0.000 claims 2
- 244000098338 Triticum aestivum Species 0.000 claims 1
- 239000007900 aqueous suspension Substances 0.000 claims 1
- 239000004495 emulsifiable concentrate Substances 0.000 claims 1
- 239000012053 oil suspension Substances 0.000 claims 1
- 150000001412 amines Chemical class 0.000 abstract description 18
- 239000000575 pesticide Substances 0.000 abstract description 12
- 230000002195 synergetic effect Effects 0.000 abstract description 6
- 239000003899 bactericide agent Substances 0.000 abstract description 5
- 238000003912 environmental pollution Methods 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 4
- VZWOXDYRBDIHMA-UHFFFAOYSA-N 2-methyl-1,3-thiazole Chemical compound CC1=NC=CS1 VZWOXDYRBDIHMA-UHFFFAOYSA-N 0.000 abstract 2
- 230000001018 virulence Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 13
- 241000209094 Oryza Species 0.000 description 12
- 244000052769 pathogen Species 0.000 description 12
- 230000001717 pathogenic effect Effects 0.000 description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 12
- 238000012360 testing method Methods 0.000 description 11
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 9
- RJQXTJLFIWVMTO-TYNCELHUSA-N Methicillin Chemical compound COC1=CC=CC(OC)=C1C(=O)N[C@@H]1C(=O)N2[C@@H](C(O)=O)C(C)(C)S[C@@H]21 RJQXTJLFIWVMTO-TYNCELHUSA-N 0.000 description 9
- 230000001580 bacterial effect Effects 0.000 description 9
- 229960003085 meticillin Drugs 0.000 description 9
- JZRWCGZRTZMZEH-UHFFFAOYSA-N Thiamine Natural products CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N JZRWCGZRTZMZEH-UHFFFAOYSA-N 0.000 description 8
- 229960003495 thiamine Drugs 0.000 description 8
- 235000019157 thiamine Nutrition 0.000 description 8
- 239000011721 thiamine Substances 0.000 description 8
- 238000002156 mixing Methods 0.000 description 7
- KYMBYSLLVAOCFI-UHFFFAOYSA-N thiamine Chemical compound CC1=C(CCO)SCN1CC1=CN=C(C)N=C1N KYMBYSLLVAOCFI-UHFFFAOYSA-N 0.000 description 7
- 238000013329 compounding Methods 0.000 description 6
- 239000013530 defoamer Substances 0.000 description 6
- 239000003814 drug Substances 0.000 description 6
- 239000000463 material Substances 0.000 description 6
- OFFFOVCCGHKJES-UHFFFAOYSA-N 4-methylthiadiazole Chemical compound CC1=CSN=N1 OFFFOVCCGHKJES-UHFFFAOYSA-N 0.000 description 5
- 241000813090 Rhizoctonia solani Species 0.000 description 5
- 238000002360 preparation method Methods 0.000 description 5
- 239000008367 deionised water Substances 0.000 description 4
- 229910021641 deionized water Inorganic materials 0.000 description 4
- 239000001963 growth medium Substances 0.000 description 4
- 238000000034 method Methods 0.000 description 4
- 241000894006 Bacteria Species 0.000 description 3
- 241000222199 Colletotrichum Species 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- SNAAJJQQZSMGQD-UHFFFAOYSA-N aluminum magnesium Chemical compound [Mg].[Al] SNAAJJQQZSMGQD-UHFFFAOYSA-N 0.000 description 3
- 239000000440 bentonite Substances 0.000 description 3
- 229910000278 bentonite Inorganic materials 0.000 description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 description 3
- OOCMUZJPDXYRFD-UHFFFAOYSA-L calcium;2-dodecylbenzenesulfonate Chemical compound [Ca+2].CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O.CCCCCCCCCCCCC1=CC=CC=C1S([O-])(=O)=O OOCMUZJPDXYRFD-UHFFFAOYSA-L 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000001939 inductive effect Effects 0.000 description 3
- 238000011835 investigation Methods 0.000 description 3
- 229920001296 polysiloxane Polymers 0.000 description 3
- WXMKPNITSTVMEF-UHFFFAOYSA-M sodium benzoate Chemical compound [Na+].[O-]C(=O)C1=CC=CC=C1 WXMKPNITSTVMEF-UHFFFAOYSA-M 0.000 description 3
- 235000010234 sodium benzoate Nutrition 0.000 description 3
- 239000004299 sodium benzoate Substances 0.000 description 3
- 229920005552 sodium lignosulfonate Polymers 0.000 description 3
- 238000010998 test method Methods 0.000 description 3
- -1 thiabendazole amine Chemical class 0.000 description 3
- 206010059866 Drug resistance Diseases 0.000 description 2
- 241001330975 Magnaporthe oryzae Species 0.000 description 2
- 241000209140 Triticum Species 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 230000000996 additive effect Effects 0.000 description 2
- 230000008485 antagonism Effects 0.000 description 2
- 230000002528 anti-freeze Effects 0.000 description 2
- 239000007798 antifreeze agent Substances 0.000 description 2
- 239000005667 attractant Substances 0.000 description 2
- IOJUPLGTWVMSFF-UHFFFAOYSA-N benzothiazole Chemical group C1=CC=C2SC=NC2=C1 IOJUPLGTWVMSFF-UHFFFAOYSA-N 0.000 description 2
- 238000004364 calculation method Methods 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 230000031902 chemoattractant activity Effects 0.000 description 2
- 238000012258 culturing Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000009036 growth inhibition Effects 0.000 description 2
- 230000028644 hyphal growth Effects 0.000 description 2
- 238000005259 measurement Methods 0.000 description 2
- 230000002265 prevention Effects 0.000 description 2
- 238000000611 regression analysis Methods 0.000 description 2
- 229920002545 silicone oil Polymers 0.000 description 2
- 231100000820 toxicity test Toxicity 0.000 description 2
- RBTBFTRPCNLSDE-UHFFFAOYSA-N 3,7-bis(dimethylamino)phenothiazin-5-ium Chemical compound C1=CC(N(C)C)=CC2=[S+]C3=CC(N(C)C)=CC=C3N=C21 RBTBFTRPCNLSDE-UHFFFAOYSA-N 0.000 description 1
- 241000228212 Aspergillus Species 0.000 description 1
- 240000006439 Aspergillus oryzae Species 0.000 description 1
- 235000002247 Aspergillus oryzae Nutrition 0.000 description 1
- 241000222235 Colletotrichum orbiculare Species 0.000 description 1
- 241000219112 Cucumis Species 0.000 description 1
- 235000010071 Cucumis prophetarum Nutrition 0.000 description 1
- 241000223195 Fusarium graminearum Species 0.000 description 1
- 241000220225 Malus Species 0.000 description 1
- 241000607479 Yersinia pestis Species 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000003905 agrochemical Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 239000011230 binding agent Substances 0.000 description 1
- 239000007767 bonding agent Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 230000006806 disease prevention Effects 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000000417 fungicide Substances 0.000 description 1
- 238000009775 high-speed stirring Methods 0.000 description 1
- 238000010902 jet-milling Methods 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 230000007246 mechanism Effects 0.000 description 1
- 125000002496 methyl group Chemical group [H]C([H])([H])* 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 239000000447 pesticide residue Substances 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 238000001228 spectrum Methods 0.000 description 1
- 238000005507 spraying Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 238000001308 synthesis method Methods 0.000 description 1
- 230000009885 systemic effect Effects 0.000 description 1
- VLLMWSRANPNYQX-UHFFFAOYSA-N thiadiazole Chemical compound C1=CSN=N1.C1=CSN=N1 VLLMWSRANPNYQX-UHFFFAOYSA-N 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
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- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention belongs to the field of agricultural bactericides, and provides a bactericidal composition containing methyl thiazole induced amine and dichlobenizox and an application thereof, wherein the mass ratio of the methyl thiazole induced amine to the dichlobenizox is 80; the bactericidal composition has excellent synergistic effect on crop diseases; can effectively reduce the dosage of pesticide, slow down the generation of disease resistance, reduce environmental pollution and is safe to crops in field observation.
Description
Technical Field
The invention belongs to the field of agricultural bactericides, and particularly relates to a bactericidal composition containing methine luramine and dichlobenizox and application thereof.
Background
The chemical agent has the advantages of obvious control effect, convenience and simplicity in use and quick response, but chemical control can cause harm to life safety of people and livestock, pesticide residue after use can cause environmental pollution, and the disease resistance can be gradually enhanced after the single agent is used for a long time. The pesticide compounding is a method for using a bactericide to reduce the generation of pesticide resistance of diseases, and particularly the bactericidal composition with a synergistic effect can delay or overcome the pesticide resistance generated after screening pesticides on pests, reduce the cost and further reduce the environmental safety problem in pesticide use.
The methyl thiadiazole induced amine is a thiadiazole plant disease prevention activator jointly developed and researched by southern university. CN100420686C discloses a synthesis method of thiamine induced amine, and also discloses that the thiamine induced amine has excellent control effects on cucumber fusarium wilt, wheat fusarium graminearum, rice aspergillus oryzae, sclerotinia sclerotiorum, apple rot pathogen, banana anthracnose pathogen, cucumber anthracnose pathogen, cotton verticillium wilt and potato late blight; CN101091490A discloses that it has an excellent control effect on cucumber downy mildew. The current research result shows that the compound has good effect of inducing disease resistance to virus diseases, and the action mechanism is inducing plants to generate systemic and broad-spectrum disease resistance activity.
Dichlobeniazox is a benzothiazole bactericide, is mainly used for preventing and treating rice diseases, particularly has good prevention effect on rice blast and sheath blight, and also has excellent prevention and treatment effect on cucumber anthracnose.
Because of the non-standard use of the existing pesticide, the resistance of the disease to the pesticide is stronger and stronger, so that the use amount of the pesticide is increased year by year, and the environmental pollution is more and more serious. Therefore, the rational use of agricultural chemicals and fungicides for preparing compositions to expand the fungicidal spectrum and reduce the resistance to diseases is imminent. No report about compounding of methionin and Dichlorobentazox exists in the prior art.
Disclosure of Invention
The invention provides a bactericidal composition with excellent synergistic effect, and the specific technical scheme is as follows:
the active ingredients of the sterilizing composition comprise the following components in percentage by mass, wherein the ratio of the methyl thia-lured amine to the Dichlorobentazox is (80).
The mass ratio of the methyl thiadiazole induced amine to the Dichlorobentazox in the bactericidal composition is (40).
The mass ratio of the methyl thiadiazole induced amine to the Dichlorobentiazox in the bactericidal composition is 20-1.
The sterilization composition comprises an auxiliary agent, and the weight percentage of the active ingredients is 1-80%. Preferably, the weight percentage of the active ingredients is 10-60%.
The auxiliary agent is one or more of wetting agent, emulsifier, dispersant, suspending agent, antifreezing agent, preservative, thickener, defoaming agent, solvent, cosolvent and filler.
The preparation of the bactericidal composition is missible oil, oil suspending agent, water suspending agent, wettable powder, water dispersible granules, microemulsion, emulsion in water and granules.
The bactericidal composition is used for preventing and treating crop diseases.
The crop diseases comprise cucumber anthracnose pathogen, rice sheath blight pathogen, rice blast pathogen, cucumber downy mildew, cucumber fusarium wilt pathogen, wheat scab pathogen, rice aspergillus, rape sclerotinia sclerotiorum, apple rot pathogen, banana anthracnose pathogen, cotton verticillium wilt pathogen, potato late blight and tobacco mosaic virus. Preferably, the crop disease is cucumber downy mildew.
By adopting the technical scheme, the invention has the following beneficial effects:
when the mass ratio of the thiabendazole amine to the dichlobeniazox is 40-1; when the mass ratio of the methicillin-induced amine to the Dichlorobentazox is 20-1. The composition can effectively reduce the dosage of pesticide, slow the generation of drug resistance of diseases and reduce environmental pollution when the composition shows synergistic action. And is safe to crops in field observation
Detailed Description
The present invention will be further explained with reference to specific embodiments.
Example 1: indoor toxicity test of cucumber anthracnose virus by compounding of methicillin-resistant amine and Dichlobeniazox
Test materials:
cucumber anthracnose pathogen (Colletotrichum lagenarium); collecting and identifying in the field, and culturing and storing indoors.
The test method comprises the following steps:
the measurement was carried out by a hyphal growth rate method. Based on the preliminary experiment, a series of PDA culture media containing medicine with concentration gradient are prepared. And cutting off the bacterial blocks along the edges of the bacterial colonies by using a puncher with the diameter of 5mm, respectively placing the bacterial blocks on the medicine-containing culture media with different concentration gradients, placing the bacterial blocks in an incubator for culture, measuring the diameters of the bacterial colonies by adopting cross when the bacterial colonies of a control group grow to 4/5 of a culture dish, and calculating the growth inhibition rate of hyphae. Corresponding regression analysis is carried out according to the concentration of each single agent and the composition and the corresponding control effect to obtain the EC of each agent 50 The value is obtained.
The bacteriostasis rate% = (diameter of control colony-diameter of treated colony)/(diameter of control colony-5) × 100%.
Calculating the co-toxicity coefficient (CTC) of the mixture by using a co-toxicity coefficient calculation method of the Sun Yunpei, and determining the synergy of the mixture.
Single agent virulence index = (EC of standard agent) 50 A certain single agent EC 50 )×100;
Theoretical virulence index (TTI) = the virulence index of A single agent multiplied by the proportion of A single agent in the mixed preparation + the virulence index of B single agent multiplied by the proportion of B single agent in the mixed preparation;
observed virulence index (ATI) = (standard single dose EC) 50 EC of value/blend 50 Value) × 100;
co-toxicity coefficient (CTC) = (measured virulence index/theoretical virulence index) × 100;
the mixture has synergistic effect when CTC is greater than 120, antagonism when CTC is less than 80, and additive effect between 80-120.
Test results
As can be seen from Table 1, EC of thiamine against Colletotrichum cucumis 50 EC value of 3.2815mg/L, dichlorobentiazox for Colletotrichum cucumerinum 50 A value of 0.2408mg/L; when the mass ratio of the methyl thiadiazole induced amine to the Dichlorobentazox is 40-1; when the mass ratio of the thiabendazole amine to the dichlobeniazox is 80.
Table 1: indoor test of compounding of methicillin-induced amine and Dichlobeniazox on cucumber colletotrichum
Example 2: indoor toxicity test of compounding of methicillin-inducing amine and Dichlobeniazox on rice sheath blight germ
Test materials:
rhizoctonia solani (Rhizoctonia solani); collecting and identifying in the field, and culturing and storing indoors.
The test method comprises the following steps:
the measurement was carried out by the hyphal growth rate method. Based on the preliminary experiment, a series of PDA culture media containing medicine with concentration gradient are prepared. And cutting off bacterium blocks along the edges of the bacterial colonies by using a puncher with the diameter of 5mm, respectively placing the bacterium blocks on medicine-containing culture media with different concentration gradients, placing the bacterium blocks in an incubator for culture, measuring the diameter of the bacterial colonies by adopting cross when the bacterial colonies of a control group grow to 4/5 of the culture dish, and calculating the hypha growth inhibition rate. Corresponding regression analysis is carried out according to the concentration of each single agent and the composition and the corresponding control effect to obtain the EC of each agent 50 The value is obtained.
Bacteriostasis% = (control colony diameter-treated colony diameter)/(control colony diameter-5) × 100%.
Calculating the co-toxicity coefficient (CTC) of the mixture by using a co-toxicity coefficient calculation method of the Sun Yunpei, and determining the synergy of the mixture.
Single dose virulence index = (EC of standard agent) 50 A single dose of EC 50 )×100;
Theoretical virulence index (TTI) = the virulence index of A single agent multiplied by the proportion of A single agent in the mixed preparation + the virulence index of B single agent multiplied by the proportion of B single agent in the mixed preparation;
observed virulence index (ATI) = (standard single dose EC) 50 EC of value/blend 50 Value) × 100;
co-toxicity coefficient (CTC) = (measured virulence index/theoretical virulence index) × 100;
the mixture has synergistic effect when CTC is greater than 120, antagonism when CTC is less than 80, and additive effect between 80-120.
Test results
As shown in Table 2, the EC of thiamine for Rhizoctonia solani 50 The value 21.4434mg/L, EC of Dichlorobentazox against Rhizoctonia solani 50 A value of 0.8096mg/L; when the mass ratio of the methicillin-induced amine to the Dichlorobentazox is 20-1; when the mass ratio of the methicillin-induced amine to the Dichlorobentazox is 80.
Table 2: indoor test of compounding methicillin-induced amine and Dichlobeniazox on rhizoctonia solani
Example 3 field control of methionin and Dichlorobentazox combinations on Pyricularia oryzae
And (3) testing crop diseases: blast of rice
Testing the medicament:
30 percent of suspending agent (comprising, for example, 25 percent of methyl thiamine, 5 percent of Dichlorobentazox, 5 percent of calcium dodecyl benzene sulfonate, 3 percent of sodium lignosulfonate, 2 percent of NNO, 2 percent of glycerol, 1.5 percent of magnesium aluminum silicate, 0.5 percent of sodium benzoate, 0.5 percent of silicone defoamer, 1 percent of bentonite and water to make up 100 percent);
25 percent of suspending agent (comprising the following components, by weight, 25 percent of methyl thiadiazole induced amine, 5 percent of calcium dodecyl benzene sulfonate, 3 percent of sodium lignosulfonate, 2 percent of NNO, 2 percent of glycerol, 1.5 percent of magnesium aluminum silicate, 0.5 percent of sodium benzoate, 0.5 percent of silicone defoamer silicone oil, 1 percent of bentonite and 100 percent of water;
5 percent of suspending agent (comprising the following components in percentage by weight: dichlorobentazox 5 percent, calcium dodecyl benzene sulfonate 5 percent, sodium lignosulfonate 3 percent, NNO 2 percent, glycerol 2 percent, magnesium aluminum silicate 1.5 percent, sodium benzoate 0.5 percent, silicone defoamer silicone oil 0.5 percent, bentonite 1 percent and water to 100 percent);
the test method comprises the following steps: refer to the pesticide field efficacy test criterion (I) that the bactericide prevents and treats the rice leaf diseases. Setting a cell: each cell area is 50m 2 Randomized block permutation, trial was repeated 4 times per treatment. The pesticide is sprayed for three times on stems and leaves of the rice in the jointing stage, the booting stage and the crevasse stage respectively, and the liquid spraying amount is 40 kg/mu; the investigation was started 30d after the third dose.
The investigation method comprises the following steps: randomly taking 5 spots per cell for investigation, 50 plants per spot, and flag leaves and two leaves below the flag leaves.
Grading standard of leaf blast:
level 0: no disease;
level 1: the number of the leaf spots is less than 5, and the length is less than 1cm;
and 3, stage: 6-10 leaf spots with length larger than 1cm;
and 5, stage: 11-25 leaf spots, wherein part of the spots are connected into pieces and occupy 10-25% of the leaf area;
and 7, stage: the number of the scab of the leaf is more than 26, and the scab is connected into pieces and occupies 26-50% of the area of the leaf;
and 9, stage: the scabs are connected into pieces and occupy more than 50% of the leaf area or are completely withered.
And (3) calculating the drug effect:
disease index = Σ [ diseased leaf number at each level × relative level value ] × 100/[ total leaf number investigated × highest level representative value ]
Control effect (%) = [ (control area disease index-treatment area disease index)/control area disease index ] × 100
Test results
As can be seen from Table 2, the control effect of the 30% suspending agent on rice blast is 92.0% compared with that of the single agent; the control effect of the compound medicament on the rice blast is obviously improved, the use amount of active ingredients can be effectively reduced, the environmental pollution is reduced, and the generation of the drug resistance of diseases is slowed down; and added observations are safe for crops.
Table 3: test of field control effect of compound agent on rice blast
Example 4 formulation example
The agent of the present invention may also be configured as the following agents:
wettable powder:
comprises the following components in percentage by weight: 1-40% of methine luramine, 1-40% of Dichlorobentazox, 1-10% of dispersing agent, 1-10% of wetting agent and the balance of filler to 100%. Mixing the thiamine attractant, the Dichlorobentazox, the dispersing agent, the wetting agent and the filler, uniformly mixing in a mixing cylinder, crushing by an airflow crusher, and uniformly mixing to obtain the thiamine attractant Dichlorobentazox wettable powder product.
Water dispersible granules:
comprises the following components in percentage by weight: 1-40% of methine luramine, 1-40% of Dichlorobentazox, 1-10% of dispersing agent, 1-10% of wetting agent, 1-5% of disintegrating agent, 1-5% of binding agent and the balance of filling material, wherein the balance is 100%. Carrying out jet milling on the methine luramine, the Dichlorobentazox, the dispersing agent, the wetting agent, the disintegrating agent, the bonding agent, the filler and the like to obtain the required particle size, thus obtaining the granulating material. Quantitatively feeding the material into a fluidized bed granulating drier, granulating and drying to obtain the product of the thiamine-induced amine dichlobbentazox water dispersible granule.
Emulsion in water:
comprises the following components in parts by weight: 1-40% of methine luramine, 1-40% of dichlobeniazox, 1-20% of solvent and cosolvent, 1-10% of emulsifier, 0.5-5% of antifreeze, 0.1-2% of thickener, 0.1-2% of defoamer and the balance of deionized water. Mixing the formula materials, and adding the methicillin technical, dichlobeniazox technical, solvent and emulsifier together to dissolve the mixture into a uniform oil phase; mixing deionized water, an antifreeze agent, a thickening agent and a defoaming agent together to form a uniform water phase. Adding the water phase into the oil phase under high-speed stirring to obtain the aqueous emulsion of methicillin.
Microemulsion:
comprises the following components in parts by weight: 1 to 40 percent of methine luramine, 1 to 40 percent of Dichlorobentazox, 1 to 30 percent of solvent and cosolvent, 5 to 30 percent of emulsifier, 0.5 to 5 percent of antifreeze, 0.1 to 2 percent of defoamer and the balance of deionized water. Dissolving a methine luramine technical and a Dichlobeniazox technical in a reaction kettle filled with a solvent and a cosolvent; adding the emulsifier, the antifreeze agent, the defoamer and the deionized water into a reaction kettle filled with the solution, and then intensively mixing and homogenizing to finally obtain the transparent methylthioninium chloride-dichlobeniazox microemulsion.
Finally, it is also noted that the above-mentioned list is only a few specific embodiments of the present invention. All modifications which can be derived or suggested from the disclosure herein by a person skilled in the art are deemed to be within the scope of the present invention.
Claims (10)
1. The sterilizing composition containing the methine luramine is characterized in that the active ingredients comprise the methine luramine and Dichlorobentiazox, and the mass ratio of the methine luramine to the Dichlorobentiazox is 80.
2. The bactericidal composition according to claim 1, wherein the mass ratio of the thiabendazole to the dichlobenizox is 40.
3. The bactericidal composition according to claim 1, wherein the mass ratio of the thiabendazole to the dichlobenizox is 20.
4. The bactericidal composition according to any one of claims 1 to 3, wherein the bactericidal composition comprises the auxiliary agent, and the active ingredient is contained in an amount of 1 to 80% by weight.
5. The bactericidal composition of claim 4, wherein the active ingredients are present in an amount ranging from 10% to 60% by weight.
6. The bactericidal composition of claim 3, wherein the auxiliary agent is one or more of a wetting agent, an emulsifying agent, a dispersing agent, a suspending agent, an antifreezing agent, a preservative, a thickening agent, an antifoaming agent, a solvent, a cosolvent, and a filler.
7. The bactericidal composition of claim 6, wherein the bactericidal composition is formulated as emulsifiable concentrate, oil suspension, aqueous suspension, wettable powder, water dispersible granules, microemulsion, aqueous emulsion, or granules.
8. Use of the fungicidal composition according to any one of claims 1 to 7 for controlling crop diseases.
9. The use of claim 8, wherein the crop diseases comprise cucumber anthracnose, rice sheath blight, rice blast, cucumber downy mildew, cucumber fusarium wilt, wheat scab, rice mold, sclerotinia sclerotiorum, apple rot, banana anthracnose, cotton verticillium wilt, potato late blight, tobacco mosaic virus.
10. The use according to claim 9, wherein the crop diseases are cucumber anthracnose, rice sheath blight and rice blast.
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