CN115932310B - Detachable sample position and chemiluminescent immunoassay device - Google Patents
Detachable sample position and chemiluminescent immunoassay device Download PDFInfo
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- CN115932310B CN115932310B CN202211683991.9A CN202211683991A CN115932310B CN 115932310 B CN115932310 B CN 115932310B CN 202211683991 A CN202211683991 A CN 202211683991A CN 115932310 B CN115932310 B CN 115932310B
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Abstract
The application discloses a detachable sample position and a chemiluminescent immunoassay device, which comprises a base and a sample tray arranged on the base, wherein a plurality of sample tubes are inserted in parallel in the circumferential direction on the sample tray, compound sample liquid coated with magnetic beads is contained in the sample tubes, an electromagnet is further arranged at the position of the sample tray, which is inserted with the sample tubes, the chemiluminescent immunoassay device further comprises a washing liquid unit, a driving assembly, a first needle tube and a second needle tube, the second needle tube is sleeved on the outer wall of the first needle tube, and the driving assembly is used for driving the first needle tube and the second needle tube to be separated from waste liquid in the sample tubes and cleaning the inner wall of the sample tubes; the application has the beneficial effects that: because the second needle tube is sleeved on the outer wall of the first needle tube, when waste liquid is pumped out, the first needle tube needs to extend into the liquid, and the second needle tube does not need to contact the waste liquid, the driving assembly drives the second needle tube to flush the compound in the sample tube, and a flushing effect is generated on the outer wall of the first needle tube.
Description
Technical Field
The application relates to the technical field related to chemiluminescence immunoassay, in particular to a detachable sample position and a chemiluminescence immunoassay device.
Background
In the known chemiluminescence immunoassay, an antigen or an antibody is directly marked by a chemiluminescent agent, the antibody which is used for coating magnetic beads excessively is used in the experimental process, the antibody, the antigen to be detected and a quantitative marked acridine ester antigen are added into a reaction cup for incubation at the same time, the marked antigen and the antibody (or the antigen to be detected and the antibody) are combined to form a complex sample liquid, and the complex and waste liquid are separated by magnetic washing after the average amount of the sample liquid is added into a plurality of sample tubes.
For example, CN102998473B, patent publication No. 2014, 11, and patent publication No. 06, discloses a full-automatic chemiluminescence immunoassay analyzer, which comprises a sample injection portion, a reaction control portion and an optical detection portion, wherein the sample injection portion comprises a reaction cup storage bin, a sample conveying device, a reagent disk, a sample diluting device, a mechanical gripper, a sample needle, a reagent needle, a magnetic bead stirring mechanism, a diluent needle and a diluent bin; the reaction control part consists of a reaction cup incubation disc and a magnetic bead cleaning disc; the optical detection part consists of an optical detection disc, a substrate needle and a substrate bin; all the components are arranged on the frame, and the sample injection part and the optical detection part are respectively positioned at two sides of the reaction control part. The application adopts an independent optical detection system, a magnetic bead cleaning system and a sample diluting system, optimizes the structure and ensures that the control is simpler. Has the characteristics of strong function, high sensitivity and low cost, and can greatly promote the development of the field of chemiluminescent immunoassay instruments.
The prior art has the defects that the injection of the cleaning liquid and the extraction of the waste liquid are carried out by two needle tubes each time, and the needle tubes for extracting the waste liquid are cleaned independently after being used.
Disclosure of Invention
The application aims to provide a detachable sample position and solve the technical problems in the related art.
In order to achieve the above object, the present application provides the following technical solutions:
can dismantle sample position, including the base and locate the sample dish on the base, a plurality of sample pipes have been inserted side by side to the circumference on the sample dish, hold the compound sample liquid of coating magnetic bead in the sample pipe, insert on the sample dish and establish the position of sample pipe still is equipped with the electro-magnet, still includes the lotion unit, and it includes drive assembly, first needle tubing and second needle tubing, the second needle tubing is overlapped and is established on the outer wall of first needle tubing, drive assembly is used for the drive first needle tubing with the second needle tubing is taken out and is left waste liquid in the sample pipe and washs the sample pipe inner wall.
Above-mentioned, drive assembly includes one and rotates locates support body on the base, offer first slide on the support body, be equipped with first slider in the first slide, first needle tubing keep away from the orificial one end rigid coupling of test tube in on the first slider, be equipped with first actuating source on the support body, first actuating source drives first slider is in remove in the first slide.
Above-mentioned, still offered the second slide on the support body, it is equipped with the second slider to slide in the second slide, the second needle tubing keep away from the orificial one end rigid coupling of test tube in on the second slider, still be equipped with the second actuating source on the support body, the second actuating source drives the second slider is in move in the second slide.
The first driving source comprises a motor, a first gear is arranged on an output shaft of the motor, a first rack is arranged on the first sliding block, the first gear is meshed with the first rack, two groups of teeth are symmetrically arranged on two sides of the first rack in the width direction, the second driving source comprises a second gear and a second rack arranged on the second sliding block, the second gear is meshed with the second rack, a friction piece is arranged at the upper end part of the second needle tube, the first needle tube is in friction contact with the friction piece, and a first limiting surface is arranged at the lower end part of the second sliding way;
the first needle tube stretches into the stroke of the sample tube, the friction piece drives the second needle tube, the second sliding block and the second rack to move downwards synchronously, when the second rack contacts with the first limiting surface, the other group of teeth in the first rack are meshed with the second gear, and the second needle tube reaches the liquid level of the sample liquid in the sample tube;
when the first rack continues to move downwards, the friction force of the friction piece is overcome, and the second rack is driven to move upwards through the second gear.
The lower port of the first needle tube is flush with the lower port of the second needle tube.
When the first needle tube drives the second needle tube to move downwards to the liquid level of the sample liquid in the sample tube through the friction piece, the lower port of the second needle tube is not contacted with the sample liquid, and when the first needle tube is contacted with the inner bottom surface of the sample tube, the lower port of the second needle tube is still positioned in the test tube.
The first rack is connected with the first needle tube, the second needle tube is connected with the first slide, the first slide is connected with the second needle tube, and the first slide is connected with the first needle tube.
The lower port of the second needle tube is provided with an inner flushing port and an outer flushing port, the inner flushing port is used for flushing the outer wall of the first needle tube, and the outer flushing port is used for flushing the inner wall of the sample tube.
The friction member is made of a soft material having elasticity.
The application also relates to a chemiluminescent immunoassay device using a detachable sample comprising the above.
The application has the beneficial effects that: because the second needle tube is sleeved on the outer wall of the first needle tube, when waste liquid is pumped out, the first needle tube needs to extend into the liquid, and the second needle tube does not need to contact the waste liquid, the driving assembly drives the second needle tube to flush the compound in the sample tube, and a flushing effect is generated on the outer wall of the first needle tube.
Drawings
In order to more clearly illustrate the embodiments of the present application or the technical solutions in the prior art, the drawings required for the embodiments will be briefly described below, and it is apparent that the drawings in the following description are only some embodiments described in the present application, and other drawings may be obtained according to these drawings for a person having ordinary skill in the art.
FIG. 1 is a schematic perspective view of a detachable sample site according to an embodiment of the present application;
FIG. 2 is a schematic cross-sectional view of a wash solution unit with a detachable sample site according to an embodiment of the present application;
FIG. 3 is an enlarged schematic view of FIG. 2A;
FIG. 4 is a schematic cross-sectional view of a wash solution unit with a detachable sample site according to another embodiment of the present application.
Reference numerals illustrate:
1. a base; 2. a sample tray; 20. a sample tube; 21. an electromagnet; 3. a washing liquid unit; 30. a drive assembly; 300. a frame body; 301. a first slideway; 302. a first slider; 303. a first gear; 304. a first rack; 305. a second slideway; 306. a second slider; 307. a second gear; 308. a second rack; 309. a friction member; 31. a first needle tube; 32. a second needle tube; 320. an inner flushing port; 321. an outer flushing port; 33. a first limiting surface; 34. the second limiting surface; 35. a first driving rope; 36. a second driving rope; 37. an elastic member; 38. inserting blocks; 4. a jacking mechanism; 40. a first upper top block; 41. a third rack; 42. a third gear; 43. a second upper top block; 44. and a third limiting surface.
Detailed Description
In order to make the technical scheme of the present application better understood by those skilled in the art, the present application will be further described in detail with reference to the accompanying drawings.
As shown in fig. 1 to 3, the detachable sample position provided by the embodiment of the application comprises a base 1 and a sample tray 2 arranged on the base 1, wherein a plurality of sample tubes 20 are inserted in parallel in the circumferential direction on the sample tray 2, the sample tray 2 is driven to intermittently rotate by a separate driving mechanism, a compound sample liquid coated with magnetic beads is contained in the sample tube 20, an electromagnet 21 is further arranged at the position of the sample tray 2 where the sample tube 20 is inserted, the base 1 further comprises a washing liquid unit 3 which comprises a driving assembly 30, a first needle tube 31 and a second needle tube 32, the second needle tube 32 is sleeved on the outer wall of the first needle tube 31, the driving assembly 30 is used for driving the first needle tube 31 and the second needle tube 32 to be separated from waste liquid in the sample tube 20 and cleaning the inner wall of the sample tube 20, and the washing liquid unit 3 is also driven to rotate by a separate driving mechanism, so that the first needle tube 31 and the second needle tube 32 can be aligned with the sample tube 20 to be cleaned up and down, namely, the second needle tube 32 can be intermittently rotated to the position of the first needle tube 31 and the second needle tube 32 can be moved to the position right under the first needle tube 31.
Specifically, the magnetic bead coated compound sample liquid has magnetism, when the sample tray 2 is intermittently rotated, the washing liquid unit 3 is rotated, so that a sample tube 20 is aligned up and down with the first needle tube 31, at this time, the electromagnet 21 is electrified to generate magnetism, and generates magnetic force which is attracted with the magnetic beads, so that the magnetic beads in the sample liquid move towards the inner wall of the sample tube 20 under the action of the magnetic force of the electromagnet 21, the magnetic bead coated compound is adhered to the inner wall of the sample tube 20 due to the blocking of the wall of the sample tube 20, the original sample liquid becomes waste liquid, the original sample liquid needs to be cleaned out, the driving component 30 drives the first needle tube 31 to extend out from the second needle tube 32 to move towards the waste liquid, the waste liquid can be gradually pumped away after the lower port of the first needle tube 31 contacts the waste liquid, and the magnetic bead coated compound is separated from the waste liquid, however, other substances may remain on the compound left, so that at this time, the driving component 30 drives the first needle tube 31 to reset and drives the second needle tube 32 to gradually go deep into the sample tube 20, when the second needle tube 32 stretches into the mouth of the sample tube 20, the electromagnet 21 is powered off, so that the compound coated with the magnetic beads falls off from the inner wall of the sample tube 20, the second needle tube 32 starts to spray flushing fluid, at this time, the first needle tube 31 is not completely separated from the sample tube 20, the second needle tube 32 flushes the inner wall of the sample tube 20 and the compound, and simultaneously flushes the outer wall of the first needle tube 31, after the flushing fluid in the sample tube 20 is basically the same as the previous waste liquid, the second needle tube 32 stops working, the driving component 30 drives the second needle tube 32 to gradually separate from the sample tube 20, at this time, the electromagnet 21 is powered on again to attract the compound coated with the magnetic beads, so that the compound is adsorbed on the inner wall of the sample tube 20, and the first needle tube 31 is driven to gradually go deep into the waste liquid formed after the flushing of the sample tube 20, the waste liquid is pumped away, the steps are repeated, and after repeated flushing for several times, the compound which basically has no other substance residues can be obtained.
The embodiment of the application has the beneficial effects that: because the second needle tube 32 is sleeved on the outer wall of the first needle tube 31, when the waste liquid is pumped out, the first needle tube 31 needs to extend into the liquid, and the second needle tube 32 does not need to contact the waste liquid, the driving assembly 30 drives the second needle tube 32 to flush the compound in the sample tube 20, and a flushing effect is generated on the outer wall of the first needle tube 31.
In one embodiment provided by the present application, preferably, the driving assembly 30 includes a frame 300 rotatably disposed on the base 1, the frame 300 is provided with a first slide 301, a first slide 302 is disposed in the first slide 301, one end of the first needle tube 31 far from the tube opening of the sample tube 20 is fixedly connected to the first slide 302, the frame 300 is provided with a first driving source, the first driving source drives the first slide 302 to move in the first slide 301 to drive the first needle tube 31 to reciprocate vertically, the frame 300 is also provided with a second slide 305, the second slide 305 is slidably provided with a second slide 306, one end of the second needle tube 32 far from the tube opening of the test tube is fixedly connected to the second slide 306, the frame 300 is also provided with a second driving source, the second driving source drives the second slide 306 to move in the second slide 305, that is, the first driving source moves in the first slide 301, and can drive the first slide 302, so as to drive the first needle tube 31 to move in and out the first slide 306, that another syringe tube 32 is easily flushed by the first syringe 32, that is easily carried out by the first driving source and the second syringe 32 is respectively, the first syringe 32 is moved in the first syringe 32 and the other embodiment, and the other syringe 32 is easily washed by the first syringe 32 is washed by the first driving source and the first syringe 32 and the other syringe 32, a set of drive sources is used to move the first needle cannula 31 and the second needle cannula 32.
The only difference between the liquid sucking and conveying process and the prior art is that a dynamic sealing structure is arranged at the connecting position of the first needle tube 31 and the second needle tube 32, the dynamic sealing structure is the prior art, all other pipeline connection, pump and control modes are not changed substantially due to the arrangement of the first needle tube 31 and the second needle tube 32, and the dynamic sealing structure can be directly used or can be used with reference to the prior art.
In a further embodiment, the first driving source includes a motor (not shown in the drawing), the output shaft of the motor drives the first gear 303 to rotate, the first slider 302 is provided with a first rack 304, the first gear 303 is engaged with the first rack 304, two sets of teeth are symmetrically disposed on two sides of the width direction of the first rack 304, the second driving source includes a second gear 307 and a second rack 308 disposed on the second slider 306, the second gear 307 is engaged with the second rack 308, a friction member 309 is disposed at an upper end portion of the second needle tube 32, the first needle tube 31 is in friction contact with the friction member 309 to bring about friction resistance, a first limit surface 33 is disposed at a lower end portion of the second slider 305, the first gear 31 extends into a stroke of the sample tube 20, the second 32, the second slider 306 and the second rack 308 are driven to move down synchronously by the friction member 309, when the second rack 308 contacts the first limit surface, the second rack 307 is driven by the second rack 308 to move down the second rack 307, and the second rack 307 is driven by the second rack 307 to move down the second rack 307 to reach the sample tube 20.
Specifically, when the waste liquid in the sample tube 20 needs to be pumped away, the motor is started, the output shaft of the motor drives the first gear 303 to rotate, and taking fig. 2 as an example, the first gear 303 rotates clockwise to drive the first rack 304 to move downwards, then the first rack 304 drives the first slider 302 to move downwards in the first slide way 301, the first slider 302 drives the first needle tube 31 to move downwards, because the first needle tube 31 contacts with the friction piece 309, the movement of the first needle tube 31 is limited by the friction resistance of the friction piece 309, the friction piece 309 is arranged at the upper end part of the second needle tube 32, the second needle tube 32 has no other limitation, the movement of the first needle tube 31 drives the second needle tube 32 to move downwards synchronously through the friction piece 309, the movement of the first needle tube 31 and the second needle tube 32 synchronously enter the sample tube 20, the movement of the second needle tube 32 drives the second slider 306 to move downwards in the second slide way 305, the second slider 306 drives the second rack 308 to move downwards synchronously, the second rack 308 drives the second gear 307 engaged with the second rack 308 to rotate clockwise, when the second rack 308 moves downwards and contacts with the first limiting surface 33, the first limiting surface 33 can prevent the second rack 308 from moving downwards, so that the second needle tube 32 can not move downwards, the motor can continue to operate, the first needle tube 31 can continue to move downwards, the first needle tube 31 overcomes the friction of the friction piece 309 and moves downwards, at the moment, the teeth on the other side of the first rack 304 are engaged with the second gear 307, the first rack 304 can drive the second gear 307 to rotate anticlockwise, the anticlockwise rotation of the second gear 307 can drive the second rack 308 engaged with the second gear 307 to move upwards, the second slider 306 drives the second needle tube 32 to move upwards in the second sliding, the first needle tube 31 and the second needle tube 32 move towards opposite directions, because the second needle tube 32 is sleeved on the outer wall of the first needle tube 31, in order to avoid that when the first needle tube 31 reaches the inner bottom surface of the sample tube 20, the second needle tube 32 can be just positioned at the tube opening of the sample tube 20 through the size design, so that when the second needle tube 32 is used for flushing the whole inner wall of the sample tube 20, the flushing length of the outer wall of the first needle tube 31 can be prolonged, preferably, the lower port of the first needle tube 31 and the lower port of the second needle tube 32 are flush when the lower port of the first needle tube 31 and the lower port of the second needle tube 32 enter the sample tube 20 together, and the lower port of the second needle tube 32 is not contacted with sample liquid (or flushing liquid generated when the second needle tube 32 is flushed later), so that the second needle tube 32 is not polluted by waste liquid, after the first needle tube 31 is pumped out of the waste liquid in the sample tube 20, the motor output shaft drives the first gear 303 to rotate anticlockwise, so that the first gear 307 is driven to rotate clockwise, the lower port of the second needle tube 32 starts to move downwards, and the inner wall of the composite needle tube 32 is flushed for a plurality of times, and the flushing operation can be completed after the inner wall of the composite needle tube is repeated.
Still further, the lower end portion of the first slide 301 is provided with a second limiting surface 34, when the first rack 304 contacts the second limiting surface 34, the first needle tube 31 contacts the inner bottom surface of the sample tube 20, and specifically, the second limiting surface 34 is provided, so that the first rack 304 can be limited when the first rack 304 contacts the second needle tube, and the motor cannot drive the first rack 304 to move downwards continuously through the first gear 303 even if the motor continues to rotate, so that the first needle tube 31 and the inner bottom surface of the sample tube 20 are prevented from being damaged by larger extrusion force generated between the first needle tube 31 and the inner bottom surface of the sample tube 20.
Preferably, the lower port of the second needle tube 32 is provided with an inner flushing port 320 and an outer flushing port 321, the inner flushing port 320 is used for flushing the outer wall of the first needle tube 31, the outer flushing port 321 is used for flushing the inner wall of the sample tube 20, specifically, if the lower port of the second needle tube 32 is in a cylindrical structure, the flushing fluid ejected from the lower port of the second needle tube 32 is also ejected in a cylindrical structure, the flushing fluid is difficult to flush the inner wall of the sample tube 20, so that the inner flushing port 320 and the outer flushing port 321 are arranged, the flushing fluid ejected from the second needle tube 32 is divided into two parts, and the flushing fluid can be flushed to the outer wall of the first needle tube 31 and the inner wall of the sample tube 20.
Preferably, the friction member 309 is made of a flexible material, and specifically, when friction is performed, so that the first needle tube 31 can drive the second needle tube 32 to move downward in the initial stage, and a sealing effect can be generated on the upper port of the second needle tube 32 by using the elastic force of the flexible material, so that when the flushing fluid enters the second needle tube 32, the flushing fluid is prevented from being sprayed out from the upper port of the second needle tube 32.
In another embodiment provided by the application, taking fig. 4 as an example, when the friction piece 309 is cancelled, that is, the motor output shaft drives the first gear 303 to rotate clockwise, the first rack 304 moves down to drive the first slider 302 to move down in the first chute, the first slider 302 drives the first needle tube 31 to move down, and the lower port of the first needle tube 31 stretches into a part of the second needle tube 32 in the initial state, in this embodiment, the position of the sample tray 2 where the test tube is inserted is further provided with the jacking mechanism 4, the frame body 300 is provided with the first driving rope 35, the second driving rope 36 and the elastic piece 37 connecting the first driving rope 35 and the second driving rope 36, the first slider 302 is inserted with the insertion block 38 in a friction manner, one end of the first driving rope 35 is fixedly connected to the insertion block 38, that is, in the process of beginning the downward movement of the first needle tube 31, the first slider 302 pulls the first driving rope 35 together to move, the first driving rope 35 pulls the elastic member 37 to move, the elastic member 37 pulls the second driving rope 36 to move, the second driving rope 36 drives power to the jacking mechanism 4, the jacking mechanism 4 jacks the sample tube 20 from the sample tray 2, in the process, the elastic member 37 generates elastic force and gradually increases, but the elastic member 37 cannot be separated from the first slider 302, when the elastic force of the elastic member 37 reaches the maximum, the friction force between the inserting member 38 and the first slider 302 is overcome, so that the elastic member 37 is separated from the first slider 302, when the liquid level of the sample liquid in the sample tube 20 is about to contact the lower port of the second needle tube 32, but the elastic member 38 cannot contact the liquid level of the sample liquid in the sample tube 20, for example, the distance between the liquid level of the sample liquid in the sample tube 20 and the pipe orifice is 8cm, when the distance between the lower port of the second needle tube 32 and the liquid level of the sample liquid is 1cm, the jacking mechanism 4 stops jacking the sample tube 20 continuously, at the moment, the elastic force of the elastic member 37 is not yet at the maximum, the elastic force of the elastic member 37 is continuously increased by the continuous downward movement of the first needle tube 31, and the first needle tube 31 extends out of the second needle tube 32 and stretches into the liquid surface to start pumping waste liquid, because the sample tube 20 moves upward, the electromagnet 21 is fixed in position, and when the sample tube 20 is not jacked by the jacking mechanism 4, the magnetic force of the electromagnet 21 can enable the compound surrounding the magnetic beads to be attached to the upper position in the sample liquid, so that the compound coated with the magnetic beads in the test tube can be gradually accumulated at the inner bottom of the sample tube 20 under the action of the magnetic force, for example, the sample liquid in the sample tube 20 is 6cm in height (the position between 5cm and 6cm when the sample tube 20 is not jacked), the compound is accumulated at the position of 1cm at the bottom, so the first needle tube 31 will not have the opportunity to draw the compound when beginning to draw the waste liquid from 6cm, wait until the first needle tube 31 reaches 2cm, at this time the elasticity of the elastic piece 37 reaches the maximum, the first needle tube 31 will still move down, then the first slider 302 breaks away from the insert 38, the jack mechanism 4 also resets gradually under the resilience force of the elastic piece 37, then the sample tube 20 returns to the initial position, under the effect of magnetic force, the compound returns to the position between 5cm and 6cm originally, at this time, when the first needle tube 31 continues to move down to draw the waste liquid, there is no compound in the residual waste liquid, after the compound in a sample tube 20 is washed, the insert 38 will be inserted with the first slider 302 again under the extrusion action in the process that the first slider 302 returns to the initial position.
During the downward movement of the first needle tube 31, the first rack 304 will also engage with the second gear 307, and the operation of the first needle tube 31 and the second needle tube 32 will be the same as described in the embodiment of the present application, and will not be described in detail herein.
The upper pushing mechanism 4 comprises a first upper pushing block 40 vertically sliding on the base 1, a third rack 41 is arranged on the first upper pushing block 40, a third gear 42 is arranged on the base 1, the third rack 41 is meshed with the third gear 42, one end of the second driving rope 36 is wound on the central shaft of the third gear 42, when the first sliding block 302 moves downwards, the second driving rope 36 pulls the third gear 42 to rotate clockwise, a second upper pushing block 43 is vertically sliding on the sample tray 2 at the position where the sample tube 20 is inserted, the first upper pushing block 40 pushes the second upper pushing block 43, the second upper pushing block 43 pushes the sample tube 20, a third limiting surface 44 is arranged on the base 1, and the third limiting surface 44 limits the upward moving distance of the first upper pushing block 40 so as to prevent the sample tube 20 from being pushed up too high.
The other embodiment provided by the application has the beneficial effects that: in the process of pumping the first needle tube 31 from the waste liquid, the compound coated with the magnetic beads is firstly moved in a concentrated manner, so that the waste liquid pumped from the first needle tube 31 does not contain the compound, and part of the compound is prevented from being carried away by the pumping force of the first needle tube 31, and the detection amount of the compound is prevented from being influenced.
In yet another embodiment, the present application is directed to a chemiluminescent immunoassay device utilizing a detachable sample comprising the above.
While certain exemplary embodiments of the present application have been described above by way of illustration only, it will be apparent to those of ordinary skill in the art that modifications may be made to the described embodiments in various different ways without departing from the spirit and scope of the application. Accordingly, the drawings and description are to be regarded as illustrative in nature and not as restrictive of the scope of the application, which is defined by the appended claims.
Claims (5)
1. Can dismantle sample position, including the base and locate the sample dish on the base, a plurality of sample pipes have been inserted side by side to the circumference on the sample dish, hold the compound sample liquid of coating magnetic bead in the sample pipe, insert on the sample dish and establish the position of sample pipe still is equipped with the electro-magnet, its characterized in that still includes:
the washing liquid unit comprises a driving assembly, a first needle tube and a second needle tube, wherein the second needle tube is sleeved on the outer wall of the first needle tube, and the driving assembly is used for driving the first needle tube and the second needle tube to be separated from waste liquid in the sample tube and cleaning the inner wall of the sample tube;
the driving assembly comprises a frame body rotationally arranged on the base, a first slideway is arranged on the frame body, a first sliding block is arranged in the first slideway, one end of the first needle tube, which is far away from the mouth of the sample tube, is fixedly connected to the first sliding block, a first driving source is arranged on the frame body, and the first driving source drives the first sliding block to move in the first slideway;
the rack body is also provided with a second slide way, a second slide block is arranged in the second slide way in a sliding way, one end of the second needle tube, which is far away from the mouth of the sample tube, is fixedly connected with the second slide block, and the rack body is also provided with a second driving source which drives the second slide block to move in the second slide way;
the first driving source comprises a motor, a first gear is arranged on an output shaft of the motor, a first rack is arranged on the first sliding block, the first gear is meshed with the first rack, two groups of teeth are symmetrically arranged on two sides of the first rack in the width direction, the second driving source comprises a second gear and a second rack arranged on the second sliding block, the second gear is meshed with the second rack, and a first limiting surface is arranged at the lower end part of the second sliding way;
the first slider is inserted with an insertion block in a friction mode, one end of the first driving rope is fixedly connected with the insertion block, namely, in the process that the first needle tube starts to move downwards, the first slider pulls the first driving rope to move together, the first driving rope pulls the elastic piece to move, the elastic piece pulls the second driving rope to move, the second driving rope transmits power to the jacking mechanism, and the jacking mechanism jacks the sample tube from the sample disc;
the upper pushing mechanism comprises a first upper pushing block vertically sliding on the base, a third rack is arranged on the first upper pushing block, a third gear is arranged on the base, the third rack is meshed with the third gear, one end of a second driving rope is wound on a central shaft of the third gear, when the first sliding block moves downwards, the second driving rope can pull the third gear to rotate clockwise, a second upper pushing block is vertically sliding on the position of the sample tray, where the sample tube is inserted, the second upper pushing block is pushed up on the first upper pushing block, the sample tube is pushed up on the second upper pushing block, a third limiting surface is arranged on the base, and the upward moving distance of the first upper pushing block is limited by the third limiting surface.
2. The removable sample site of claim 1, wherein in an initial state, the first syringe lower port is flush with the lower port of the second syringe.
3. The detachable sample site of claim 1, wherein the lower end of the first slide is provided with a second limiting surface, and the first needle cannula contacts the bottom surface within the sample tube when the first rack contacts the second limiting surface.
4. The detachable sample site of claim 1, wherein the lower port of the second needle cannula is provided with an inner flushing port for flushing the outer wall of the first needle cannula and an outer flushing port for flushing the inner wall of the sample tube.
5. Chemiluminescent immunoassay device comprising a detachable sample site according to any one of claims 1-4.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211683991.9A CN115932310B (en) | 2022-12-27 | 2022-12-27 | Detachable sample position and chemiluminescent immunoassay device |
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211683991.9A CN115932310B (en) | 2022-12-27 | 2022-12-27 | Detachable sample position and chemiluminescent immunoassay device |
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| CN115932310A CN115932310A (en) | 2023-04-07 |
| CN115932310B true CN115932310B (en) | 2023-08-18 |
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Citations (25)
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2022
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