CN115193812A - Cleaning station system of chemiluminescence detector - Google Patents
Cleaning station system of chemiluminescence detector Download PDFInfo
- Publication number
- CN115193812A CN115193812A CN202210895095.2A CN202210895095A CN115193812A CN 115193812 A CN115193812 A CN 115193812A CN 202210895095 A CN202210895095 A CN 202210895095A CN 115193812 A CN115193812 A CN 115193812A
- Authority
- CN
- China
- Prior art keywords
- reaction cup
- cleaning
- magnet
- needle
- magnets
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
Images
Classifications
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/10—Cleaning involving contact with liquid with additional treatment of the liquid or of the object being cleaned, e.g. by heat, by electricity or by vibration
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/04—Cleaning involving contact with liquid
- B08B3/08—Cleaning involving contact with liquid the liquid having chemical or dissolving effect
Landscapes
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Automatic Analysis And Handling Materials Therefor (AREA)
Abstract
The invention discloses a cleaning station system of a chemiluminescence detector, which comprises a cleaning needle assembly, a reaction cup rotating module, a magnetic bead adsorption assembly and a controller module. The magnetic bead adsorption component is used for adsorbing magnetic beads on the wall of the reaction cup when the cleaning needle assembly extracts waste liquid, and the magnetic beads are dispersed in the reaction cup when the liquid is pumped and cleaned. Control module combines the sensor among the washing needle subassembly, whether can accurately detect and squeeze into quantitative cleaning liquid with full pipe state when beating liquid to and whether take place the stifled needle condition when taking out the waste liquid, avoid because the magnetic bead washs final detection effect of thorough influence. Simultaneously, reaction cup rotates the module and utilizes the magnet of reaction cup support bottom and reaction cup rolling disc magnet between the interact, rotates the in-process at the reaction cup rolling disc, realizes the effective mixing of magnetic bead in the reaction cup and washs to make when inhaling the waste liquid the magnetic bead adsorbed on the minor face of reaction cup, when being compared in the long limit of adsorbing in the reaction cup, reduce the magnetic loss, avoid appearing the stifled needle condition.
Description
Technical Field
The invention relates to a cleaning station system of a chemiluminescence detector.
Background
The principle of chemiluminescence is to excite an intermediate using free energy released by a chemical reaction, returning it from an excited state to a ground state. Common intermediates are alkaline phosphatase-amantadine, horseradish peroxidase-luminol derivatives, and horseradish peroxidase-luminol derivatives. When the intermediate returns from the excited state to the ground state, photons of an isolevel are released, and the photons are measured and quantitatively analyzed. Chemiluminescence has fluorescence specificity, does not need exciting light, avoids the influence of stray light of the exciting light in fluorescence analysis, improves sensitivity, avoids environmental pollution and health hazard caused by radiation analysis, and is an excellent quantitative analysis method.
The magnetic particle chemiluminescence immune analysis is an analysis method combining magnetic separation technology, chemiluminescence technology and immune analysis technology. The technology fully utilizes the rapid and easy automation of the magnetic separation technology, the high sensitivity of the chemiluminescence technology and the specificity of immunoassay, and shows irreplaceable effects in the field of bioanalysis.
At present, the magnetic particle chemical analysis immunoassay is applied to a tubular chemiluminescence immunoassay item and an electrochemical luminescence immunoassay item. Tubular chemiluminescence is currently the most rapidly developing chemiluminescence detection instrument. Although the types of magnetic particles used for chemiluminescence are various at present, the most basic requirements are fast magnetic response, monodispersity, uniform particle size, good suspension property, low non-specific adsorption, good stability and the like, so that the magnetic polystyrene microspheres are most commonly used.
Aiming at different principles of chemiluminescence detection, the main application methods of magnetic beads in the field of chemiluminescence include an indirect method, a capture method, a solid-phase antigen competition method, a double-antibody sandwich method and the like. Wherein the indirect method comprises: a) Fixing antigen on the surface of the magnetic microsphere; b) Adding a sample (containing a target antibody), incubating and washing; c) Adding enzyme-labeled anti-antibody for incubation and cleaning; d) Adding a luminescent substrate, and detecting a signal. The capture method comprises the following steps: a) Connecting the IgM antibody to a solid phase carrier to form solid-phase IgM, and cleaning; b) Adding diluted serum, capturing IgM antibodies in the sample by solid phase antibodies, and cleaning to remove other immunoglobulins and impurity components in the serum; c) Adding specific antigen reagent which is only combined with the specific IgM on the solid phase, and cleaning; d) Adding enzyme-labeled antibody aiming at specificity, reacting and combining the enzyme-labeled antibody with the antigen combined on the solid phase, and cleaning; e) Adding substrate for color development, and detecting signals. The solid phase antigen competition method comprises: a) Fixing specific antigen on the surface of the magnetic bead; b) Adding a mixed solution of a detected sample and the enzyme-labeled antigen for incubation; c) Adding a luminescent substrate, and detecting a signal. The double antibody sandwich method comprises the following steps: a) Fixing an antibody on the surface of the magnetic bead; b) Adding a sample and an enzyme-labeled secondary antibody for incubation and cleaning; c) Adding a luminescent substrate, and detecting a signal.
In the above method, the magnetic microspheres (magnetic beads) need to be washed with a washing solution during the chemiluminescence detection process, and the waste liquid needs to be extracted after washing. According to the chemiluminescence and detection principle thereof, whether the magnetic bead cleaning is thorough or not directly influences the final detection accuracy. Among the current chemiluminescence detector, the washing needle for squeezing into the washing liquid and extracting the waste liquid all adopts the sleeve structure, and the waste liquid needle at center is used for absorbing the waste liquid promptly, and the liquid injection needle that coaxially cup joints on the waste liquid needle is used for injecting liquid, and waste liquid pipeline and washing liquid pipeline are connected respectively to the top of waste liquid needle and liquid injection needle. In practice, it is found that before and after the cleaning solution substrate bottle is replaced, bubbles appear in a cleaning solution pipeline during solution pumping, namely, the cleaning solution enters the reaction cup in a gas-liquid mixed state, and the situation directly causes that the amount of the cleaning solution injected into the reaction cup at this time is less than a set amount, so that the cleaning condition of the magnetic beads does not reach the standard. In addition, when waste liquid is extracted, the granular magnetic beads are often extracted along with the waste liquid, and if the magnetic beads excessively enter the waste liquid needle along with the waste liquid at the same time, the waste liquid needle is often blocked, namely the needle is blocked; if the waste liquid in the reaction cup is not completely extracted, the result of the subsequent measurement process is directly influenced.
In addition, in the existing chemiluminescence detector, because all parts are highly integrated and are compactly distributed in a limited space, a two-claw mechanical clamping jaw clamp for clamping a reaction cup cannot horizontally rotate the clamping jaw angle. When the magnetic beads are cleaned, the reaction cup filled with the magnetic beads is clamped by the two-claw mechanical clamping jaw and then vertically and downwards inserted into a reaction cup support positioned on the reaction cup rotating module for taking and placing the cup position. And the cleaning needle for injecting cleaning liquid and extracting waste liquid is arranged above the cleaning device and is driven by a motor to move up and down along the vertical direction.
Disclosure of Invention
The invention aims to: to the prior art, a cleaning station system of the chemiluminescence detector is provided, and the cleaning effect of magnetic beads is improved.
The technical scheme is as follows: a cleaning station system of a chemiluminescence detector comprises a cleaning needle assembly, a reaction cup rotating module, a magnetic bead adsorption assembly and a controller module; the cleaning needle assembly is arranged above the reaction cup rotating module and is driven by a motor to move up and down; the magnetic bead adsorption assembly is used for adsorbing magnetic beads on the wall of the reaction cup when the cleaning needle assembly extracts waste liquid, and dispersing the magnetic beads in the reaction cup when liquid pumping cleaning is carried out;
the cleaning needle assembly further comprises a pressure sensor connected in series with the waste liquid pipeline and a non-contact liquid sensor arranged beside the cleaning liquid pipeline, and the pressure sensor and the non-contact liquid sensor are connected with the controller module; when the controller module detects that the pressure of the waste liquid pipeline is smaller than a set threshold value, a needle blocking signal is output; and the controller module judges three states of bubbles, empty pipes and full pipes of the cleaning liquid pipeline according to the output signals of the non-contact liquid sensor and correspondingly outputs state signals when liquid drops or bubbles are in a state.
Further, the controller module judges three states of bubble, empty pipe and full pipe of the cleaning liquid pipeline according to the voltage change output by the non-contact liquid sensor, and specifically includes:
sampling the output value of the non-contact liquid sensor in the empty pipe state of the cleaning liquid pipeline to obtain a reference value of the empty pipe state;
continuously sampling the output value of the non-contact liquid sensor, calculating the duty ratio of an oscillation signal in unit time, and if the oscillation duty ratio of the signal is greater than a threshold value, determining that the signal is in a bubble state;
and continuously sampling the output value of the non-contact liquid sensor, and if the difference value of two adjacent sampling values is smaller than a threshold value and the sampling values are larger than the reference value of the empty pipe state, determining that the pipe is in the full pipe state.
Furthermore, the reaction cup rotating module comprises a bottom plate, a reaction cup bracket, a reaction cup rotating disc and a first motor; the reaction cup rotating disc is arranged above the bottom plate in parallel, and the first motor is used for driving the reaction cup rotating disc to rotate around the central shaft; a plurality of first through holes are distributed on the reaction cup rotating disc at intervals along the circumference;
a plurality of first magnets are distributed on the bottom plate at intervals along the circumference, the magnetic pole directions of the first magnets are arranged along the radial direction of the bottom plate, the S pole is positioned at the inner side, and the N pole is positioned at the outer side; the bottom plate is also provided with a second magnet, and the second magnet is positioned between two adjacent first magnets and is opposite to the cup taking and placing position;
a reaction cup accommodating groove with a rectangular cross section is formed in the reaction cup support, a rotating shaft is vertically fixed at the bottom of the reaction cup support, a rotating bearing is connected to the rotating shaft, the rotating bearing is embedded into a first through hole in a reaction cup rotating disc, and the lower end of the rotating shaft penetrates through the reaction cup rotating disc and is fixedly connected with a first magnet fixing block; a third magnet is arranged on the first magnet fixing block, and the magnetic pole direction of the third magnet is consistent with the long axis direction of the cross section of the reaction cup accommodating groove; the third magnet, the first magnet and the second magnet are all positioned on the same horizontal circumference;
the magnetic bead adsorption component comprises a plurality of fourth magnets which are arranged on the inner side of the reaction cup support and are arranged at intervals along the circumference.
Further, in the magnetic bead adsorption assembly, the fourth magnets are respectively fixed on second magnet fixing blocks, the second magnet fixing blocks are connected with driving structures, and the driving structures drive the second magnet fixing blocks to move along the radial direction.
The mixing component comprises a third motor, at least one second through hole positioned between two adjacent first magnets is further formed in the bottom plate, a third magnet fixing block is embedded into the second through hole, a fifth magnet is arranged on the third magnet fixing block, and the fifth magnet, the first magnet, the second magnet and the third magnet are all positioned on the same horizontal circumference; and the third motor is arranged below the bottom plate and used for driving the third magnet fixing block to rotate.
Furthermore, the driving structure comprises a plurality of supporting rods, a chassis and a second motor, wherein a plurality of T-shaped sliding grooves are formed in the chassis along the radial direction, each second magnet fixing block is fixedly connected with one end of one supporting rod, the main body of each supporting rod is correspondingly inserted into one T-shaped sliding groove in the chassis, and the output shaft of the second motor is connected with each supporting rod through a connecting rod transmission mechanism.
Further, the vertical distance between the third magnet and the bottom of the reaction cup in the reaction cup accommodating groove is more than or equal to 30 mm.
Further, the cleaning needle assembly comprises a cleaning needle bracket, a fourth motor, a cleaning needle and a sensor fixing plate; the cleaning needle is vertically fixed on the cleaning needle support, and the fourth motor drives the cleaning needle support to move up and down so as to drive the cleaning needle to move up and down; the sensor fixing plate is arranged beside the cleaning needle support and used for fixing a pressure sensor, a non-contact liquid sensor and a pipeline connected with the cleaning needle.
Has the advantages that: 1. the cleaning station system of the chemiluminescence detector can accurately detect whether quantitative cleaning liquid is injected in a full pipe state during liquid injection and whether needle blockage occurs during waste liquid extraction, and avoids the final detection effect from being influenced because magnetic beads are not thoroughly cleaned.
2. According to the invention, the interaction between the magnet at the bottom of the reaction cup support and the magnet of the reaction cup rotating disc is utilized, so that the effective uniform mixing and cleaning of the magnetic beads in the reaction cup are realized in the rotating process of the reaction cup rotating disc, the magnetic beads are adsorbed on the short side of the reaction cup when the waste liquid is absorbed, and compared with the situation that the magnetic beads are adsorbed on the long side of the reaction cup, the situation that the magnetic beads excessively enter the waste liquid needle along with the waste liquid at the same moment is avoided, and the magnetic loss can be reduced. Compare in the distance of reaction cup support through adjustment fourth magnet, can further improve magnetic bead cleaning performance to further avoid inhaling the magnetic loss when the waste liquid. The dynamic uniform mixing of the magnetic beads in the cleaning liquid is realized through the uniform mixing component, and the cleaning effect of the magnetic beads is further improved.
Drawings
FIG. 1 is a schematic perspective view of a cleaning station system of the present invention;
FIG. 2 is a schematic top view of the cleaning station system of the present invention with sensor components omitted;
FIG. 3 is a schematic bottom view of the cleaning station system of the present invention;
FIG. 4 is a schematic view of the distribution and driving structure of the fourth magnet in the cleaning station system of the present invention;
FIG. 5 is a schematic diagram of the construction of the blending assembly of the cleaning station system of the present invention;
FIG. 6 is a schematic diagram of the structure of the cuvette holder in the system of the cleaning station according to the present invention.
Detailed Description
The invention is further explained below with reference to the drawings.
Example 1:
a cleaning station system of a chemiluminescence detector comprises a cleaning needle assembly, a reaction cup rotating module, a magnetic bead adsorption assembly and a controller module. The cleaning needle assembly is arranged above the cup rotating module and is driven by a motor to move up and down. The magnetic bead adsorption component adsorbs the magnetic bead on the wall of the reaction cup when being used for cleaning waste liquid extracted by the needle assembly, and the magnetic bead is dispersed in the reaction cup when the liquid is pumped and cleaned. The cleaning needle assembly also comprises a pressure sensor connected in series with the waste liquid pipeline and a non-contact liquid sensor arranged beside the cleaning liquid pipeline, and the pressure sensor and the non-contact liquid sensor are connected with a controller module. And when the controller module detects that the pressure of the waste liquid pipeline is less than a set threshold value, a needle blocking signal is output. The controller module judges three states of bubbles, empty pipes and full pipes of the cleaning liquid pipeline according to the output signals of the non-contact liquid sensor, and correspondingly outputs state signals when the liquid drops or the bubbles are in a state.
As shown in fig. 1 to 3, the cuvette rotating module includes a base plate 1, a cuvette holder 2, a cuvette rotating plate 3, and a first motor 4. Reaction cup rolling disc 3 parallel arrangement is in bottom plate 1 top, and first motor 4 sets up in bottom plate 1 below, and reaction cup rolling disc 3 central authorities are fixed with the center pin, and first motor 4 is used for driving the center pin and rotates to drive reaction cup rolling disc 3 around the center pin rotates. A plurality of first through holes 5 are distributed on the reaction cup rotating disc 3 at intervals along the circumference.
A plurality of first magnets 6 are distributed on the bottom plate 1 at intervals along the circumference, the magnetic pole direction of the first magnets 6 is arranged along the radial direction of the bottom plate 1, the S pole is positioned on the inner side, and the N pole is positioned on the outer side. The bottom plate 1 is also provided with a second magnet 7, and the second magnet 7 is positioned between two adjacent first magnets 6 and is opposite to the cup taking and placing position. In this embodiment, the magnetic pole direction of the second magnet 7 is perpendicular to the radial direction of the base plate 1 so as to correspond to the included angle of the two-jaw mechanical clamping jaw.
As shown in figure 6, be equipped with the reaction cup holding tank that the cross section is rectangular on reaction cup support 2, the vertical fixed pivot in bottom of reaction cup support 2 is connected with rolling bearing 8 in the pivot, and rolling bearing 8 is embedded into the first through-hole 5 on the reaction cup rolling disc 3, and the first magnet fixed block 9 of reaction cup rolling disc 3 and fixedly connected with is worn out to the pivot lower extreme. Third magnet 10 is embedded into the first magnet fixing block 9, and the magnetic pole direction of the third magnet 10 is consistent with the long axis direction of the cross section of the reaction cup accommodating groove. The third magnet 10 is located on the same horizontal circumference as the first magnet 6 and the second magnet 7.
The magnetic bead adsorption component comprises a plurality of fourth magnets 11 which are arranged on the inner side of the reaction cup support 2 and are arranged along the circumference at intervals.
The cleaning needle assembly comprises a cleaning needle bracket 18, a fourth motor 19, a cleaning needle 20 and a sensor fixing plate 27. The cleaning needle 20 is vertically fixed on the cleaning needle support 18, and the fourth motor 19 drives the cleaning needle support 18 to move up and down, so as to drive the cleaning needle 20 to move up and down. The sensor fixing plate 27 is disposed beside the cleaning needle holder 18 for fixing the pressure sensor 28, the non-contact liquid sensor, and the pipe line 29 connected to the cleaning needle 20.
In the structure of the invention, the reaction cup 21 with magnetic beads is clamped by the two-jaw mechanical clamping jaw and then vertically inserted downwards into the reaction cup support positioned on the reaction cup rotating module for taking and placing the cup position, in the embodiment, when the reaction cup 21 is positioned at the cup taking and placing position, the long edge of the cross section of the reaction cup 21 is vertical to the radial direction of the reaction cup rotating disc 3; the first motor 4 drives the reaction cup rotating disc 3 to rotate, so as to drive the reaction cups 21 in the reaction cup support for taking and placing the cup positions to rotate along the circumference, the third magnets 10 arranged at the bottom of the reaction cup support 2 and the first magnets 6 distributed on the reaction cup rotating disc 3 at intervals along the circumference generate attraction and repulsion effects, the reaction cup support 2 generates autorotation in the process of reaching the next first magnet 6 position, and the reaction cups 21 are switched into short sides of the cross sections of the reaction cups 21 to be vertical to the radial direction of the reaction cups 21 along the reaction cup rotating disc 3 when reaching the next first magnet 6 position; in the process that the reaction cup support 2 rotates from the position of one first magnet 6 to the position of the next first magnet 6, the reaction cup support 2 rotates, the short side of the cross section of the reaction cup 21 is always kept perpendicular to the reaction cup 21 in the radial direction of the reaction cup rotating disc 3 at the position of the first magnet 6, and meanwhile, the same short side of the cross section of the reaction cup 21 is always kept close to the circle center of the reaction cup rotating disc 3. In the above process, the reaction cup 21 continuously rotates in the process of driving the reaction cup rotating disc 3 to move, and the distance and the relative position between the magnetic bead in the reaction cup 21 and the fourth magnet 11 change during rotation, so that the magnetic bead generates a moving track in the reaction cup 21, the magnetic bead is in better contact with a cleaning solution in the reaction cup, and the magnetic bead is more thoroughly cleaned.
When the reaction cup support 2 rotates to the first magnet 6 just below the cleaning needle 20, under the action of the fourth magnet 11, the magnetic beads are adsorbed on the one side of the short side of the reaction cup 21, the cleaning needle 20 moves downwards to be inserted into the center of the reaction cup 21, and because the angle of the reaction cup support 2 is fixed at the moment, the magnetic beads are adsorbed on the one side of the short side of the reaction cup 21 and are farther away from the cleaning needle 20 compared with the situation that the magnetic beads are adsorbed on the one side of the short side of the reaction cup 21, the magnetic beads adsorbed on the farther short side of the distance can be slightly influenced when the cleaning needle 20 absorbs waste liquid, so that the magnetic loss is reduced, and the situation that the magnetic beads are excessively introduced into the waste liquid needle at the same moment and block the needle can be further avoided. When the reaction cup support 2 returns to the cup taking and placing position after rotating for one circle, the long edge of the cross section of the reaction cup 21 is recovered to be vertical to the reaction cup 21 along the radial direction of the reaction cup rotating disc 3, so that the two-jaw mechanical clamping jaw clamp is convenient to take and place the reaction cup 21.
Preferably, the vertical distance between the third magnet 10 and the bottom of the cuvette in the cuvette-holding groove is not less than 30 mm in order to avoid the influence of the third magnet 10 on the magnetic beads in the cuvette 21. The bottom plate 1 is also provided with a first optocoupler 23 for detecting the rotation angle of the reaction cup rotating disc 3.
In the invention, the controller module judges three states of bubble, empty pipe and full pipe of the cleaning liquid pipeline according to the voltage change output by the non-contact liquid sensor, and the method specifically comprises the following steps:
and sampling the output value of the non-contact liquid sensor in the empty pipe state of the cleaning liquid pipeline to obtain a reference value of the empty pipe state. In this embodiment, the method for calculating the reference value of the empty pipe state includes acquiring the output value of the non-contact liquid sensor n times, and calculating an average value of sampling after removing the maximum value and the minimum value:
wherein the content of the first and second substances,
the reference value is in an empty pipe state, ai is the sampling value of the ith time, amin is the minimum value of sampling, and Amax is the maximum value of sampling; n is generally 10 or more.
Continuously sampling the output value of the non-contact liquid sensor, calculating the duty ratio of an oscillation signal in unit time, and if the oscillation duty ratio of the signal is greater than a threshold value P 2 Then, it is determined as a bubble state. That is, the present invention continuously samples, and if the difference between the current sampling value Ai and the previous sampling value A (i-1) is greater than the threshold value P 1 Then, the oscillation signal appears, and the continuous oscillation signal appears, namely, the bubble state of gas-liquid mixing appears in the pipeline, wherein, the threshold value P 1 And a threshold value P 2 The value of (b) can be obtained by experimental calibration.
Continuously sampling the output value of the non-contact liquid sensor if two adjacent liquid sensors are adjacentThe difference values of the sub-sampling values are all smaller than the threshold value P 3 And the sampling values are all larger than the reference value of the empty pipe state
It is determined as a full pipe state. That is, in the full tube state, the detection value of the non-contact liquid sensor is a non-oscillation signal. Threshold value P 3 The difference is different according to the sensor, and the detection precision value of the sensor is generally 2 times.
The invention adopts two types of sensors to respectively detect the waste liquid pipeline and the cleaning liquid pipeline, when a waste liquid needle is blocked or bubbles appear in the cleaning liquid pipeline during liquid injection, the controller sends out corresponding prompt signals, and can select to eliminate the influenced chemiluminescence detection value according to the related prompt signals, namely, the detection value with possibly influenced precision is excluded, so as to ensure the accuracy of the final output result of the instrument.
Example 2:
the difference from embodiment 1 is only that, in the magnetic bead adsorption assembly, the fourth magnets 11 are respectively fixed on the second magnet fixing blocks 12, and the second magnet fixing blocks 12 are connected with a driving structure, and the driving structure drives the second magnet fixing blocks 12 to displace along the radial direction.
Through adjusting fourth magnet 11 in radial ascending position, adjust relative reaction cup 21's distance promptly, can realize that reaction cup 21 from just rotating 6 position in-process of next first magnet to 6 position of a first magnet, the realization that the magnetic bead can be better is broken up and is gathered, is favorable to the better and washing liquid contact of magnetic bead.
When the reaction cup support 2 rotates to the first magnet 6 which is right opposite to the lower part of the cleaning needle 20 and the cleaning needle 20 sucks waste liquid, the fourth magnet 11 is controlled to be closer to the reaction cup 21, so that the suction force to magnetic beads is enhanced, and the magnetic loss during sucking the waste liquid is further avoided; when the cleaning needle 20 is used for pumping liquid, the fourth magnet 11 is controlled to be far away from the reaction cup 21, so that magnetic beads are dispersed, comprehensive contact between the magnetic beads and cleaning liquid is facilitated, and the magnetic bead cleaning effect is improved.
Specifically, as shown in fig. 4, the driving structure includes a plurality of supporting rods 13, a chassis 14, and a second motor 15, the chassis 14 is radially provided with a plurality of T-shaped sliding grooves 24, each second magnet fixing block 12 is respectively and fixedly connected to one end of one supporting rod 13, a main body of each supporting rod 13 is respectively and correspondingly inserted into one T-shaped sliding groove 24 on the chassis 14, and an output shaft of the second motor 15 is connected to each supporting rod 13 through a connecting rod transmission mechanism. And a second optical coupler 25 for detecting the moving position of the support rod 13 is also arranged on each T-shaped sliding groove 24.
Example 3:
as shown in fig. 5, the device is different from the embodiment 2 only in that the device further comprises a blending component, the blending component comprises a third motor 16, at least one second through hole located between two adjacent first magnets 6 is further formed in the bottom plate 1, a third magnet fixing block 17 is embedded in the second through hole, a fifth magnet is arranged on the third magnet fixing block 17, and the third motor 16 is arranged below the bottom plate 1 and used for driving the third magnet fixing block 17 to rotate.
When the reaction cup support 2 rotates to face the third magnet fixing block 17, the fourth magnet 11 is controlled to be away from the reaction cup 21, the third magnet fixing block 17 is driven to rotate to drive the fifth magnet to rotate, the reaction cup support 2 is driven to rotate by the attraction between the fifth magnet and the third magnet 10, dynamic mixing of magnetic beads in cleaning liquid is achieved, and the magnetic bead cleaning effect is improved.
If the mixing subassembly sets up in the below just to wasing needle 20, the magnetic bead adsorbs all the time in the minor face of reaction cup when guaranteeing the imbibition, and this mixing subassembly still includes the third opto-coupler 22 that is used for detecting the 17 turned angle of third magnet fixed block to and with the opto-coupler separation blade 26 of 17 pivot fixed connection of third magnet fixed block.
The foregoing is only a preferred embodiment of the present invention, and it should be noted that, for those skilled in the art, various modifications and decorations can be made without departing from the principle of the present invention, and these modifications and decorations should also be regarded as the protection scope of the present invention.
Claims (8)
1. A cleaning station system of a chemiluminescent detector is characterized by comprising a cleaning needle assembly, a reaction cup rotating module, a magnetic bead adsorption assembly and a controller module; the cleaning needle assembly is arranged above the reaction cup rotating module and is driven by a motor to move up and down; the magnetic bead adsorption assembly is used for adsorbing magnetic beads on the wall of the reaction cup when the cleaning needle assembly extracts waste liquid, and dispersing the magnetic beads in the reaction cup when the liquid is extracted and cleaned;
the cleaning needle assembly also comprises a pressure sensor connected in series with the waste liquid pipeline and a non-contact liquid sensor arranged beside the cleaning liquid pipeline, and the pressure sensor and the non-contact liquid sensor are connected with the controller module; when the controller module detects that the pressure of the waste liquid pipeline is smaller than a set threshold value, a needle blocking signal is output; and the controller module judges three states of bubbles, an empty pipe and a full pipe of the cleaning liquid pipeline according to the output signal of the non-contact liquid sensor and correspondingly outputs a state signal when the liquid drops or the bubbles are in a state.
2. The system of claim 1, wherein the controller module determines three states of bubble, empty pipe and full pipe of the cleaning solution pipeline according to the voltage variation output by the non-contact liquid sensor, and specifically comprises:
sampling the output value of the non-contact liquid sensor in the empty pipe state of the cleaning liquid pipeline to obtain a reference value of the empty pipe state;
continuously sampling the output value of the non-contact liquid sensor, calculating the duty ratio of an oscillation signal in unit time, and if the oscillation duty ratio of the signal is greater than a threshold value, determining that the signal is in a bubble state;
and continuously sampling the output value of the non-contact liquid sensor, and if the difference value of the two adjacent sampling values is smaller than the threshold value and the sampling values are larger than the reference value of the empty pipe state, judging that the pipe is in a full pipe state.
3. The cleaning station system of the chemiluminescence detector according to claim 1 or 2, wherein the cuvette rotating module comprises a base plate (1), a cuvette holder (2), a cuvette rotating disk (3), and a first motor (4); the reaction cup rotating disc (3) is arranged above the bottom plate (1) in parallel, and the first motor (4) is used for driving the reaction cup rotating disc (3) to rotate around a central shaft; a plurality of first through holes (5) are distributed on the reaction cup rotating disc (3) at intervals along the circumference;
a plurality of first magnets (6) are distributed on the bottom plate (1) at intervals along the circumference, the magnetic pole directions of the first magnets (6) are arranged along the radial direction of the bottom plate (1), the S pole is positioned at the inner side, and the N pole is positioned at the outer side; the bottom plate (1) is also provided with a second magnet (7), and the second magnet (7) is positioned between two adjacent first magnets (6) and is opposite to the cup taking and placing positions;
a reaction cup accommodating groove with a rectangular cross section is formed in the reaction cup support (2), a rotating shaft is vertically fixed at the bottom of the reaction cup support (2), a rotating bearing (8) is connected to the rotating shaft, the rotating bearing (8) is embedded into a first through hole (5) in the reaction cup rotating disc (3), and the lower end of the rotating shaft penetrates through the reaction cup rotating disc (3) and is fixedly connected with a first magnet fixing block (9); a third magnet (10) is arranged on the first magnet fixing block (9), and the magnetic pole direction of the third magnet (10) is consistent with the long axis direction of the cross section of the reaction cup accommodating groove; the third magnet (10), the first magnet (6) and the second magnet (7) are all positioned on the same horizontal circumference;
the magnetic bead adsorption component comprises a plurality of fourth magnets (11) which are arranged on the inner side of the reaction cup support (2) at intervals along the circumference.
4. The cleaning station system of the chemiluminescent detector according to claim 3, wherein the magnetic bead adsorption assembly comprises fourth magnets (11) respectively fixed on second magnet fixing blocks (12), and the second magnet fixing blocks (12) are connected with a driving structure, and the driving structure drives the second magnet fixing blocks (12) to displace along a radial direction.
5. The cleaning station system of the chemiluminescence detector according to claim 4, further comprising a blending component, wherein the blending component comprises a third motor (16), the bottom plate (1) is further provided with at least one second through hole located between two adjacent first magnets (6), a third magnet fixing block (17) is embedded in the second through hole, a fifth magnet is arranged on the third magnet fixing block (17), and the fifth magnet, the first magnets (6), the second magnets (7) and the third magnets (10) are all located on the same horizontal circumference; the third motor (16) is arranged below the bottom plate (1) and used for driving the third magnet fixing block (17) to rotate.
6. The cleaning station system of the chemiluminescence detector according to claim 4, wherein the driving structure comprises a plurality of support rods (13), a chassis (14), and a second motor (15), wherein the chassis (14) is provided with a plurality of T-shaped sliding grooves along a radial direction, each second magnet fixing block (12) is fixedly connected with one end of one support rod (13), the main bodies of the support rods (13) are correspondingly inserted into one T-shaped sliding groove on the chassis (14), and the output shaft of the second motor (15) is connected with each support rod (13) through a connecting rod transmission mechanism.
7. The cleaning station system of chemiluminescence detection apparatus according to any of claims 4 to 6, wherein the vertical distance between the third magnet (10) and the bottom of the cuvette in the cuvette-accommodating groove is 30 mm or more.
8. The washing station system of the chemiluminescent detector according to any one of claims 4 to 6 wherein the washing needle assembly comprises a washing needle holder (18), a fourth motor (19), a washing needle (20), a sensor fixing plate (27); the cleaning needle (20) is vertically fixed on the cleaning needle support (18), and the fourth motor (19) drives the cleaning needle support (18) to move up and down so as to drive the cleaning needle (20) to move up and down; the sensor fixing plate (27) is arranged beside the cleaning needle bracket (18) and is used for fixing pipelines connected with the pressure sensor, the non-contact liquid sensor and the cleaning needle (20).
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210895095.2A CN115193812B (en) | 2022-07-28 | 2022-07-28 | Cleaning station system of chemiluminescent detector |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210895095.2A CN115193812B (en) | 2022-07-28 | 2022-07-28 | Cleaning station system of chemiluminescent detector |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115193812A true CN115193812A (en) | 2022-10-18 |
| CN115193812B CN115193812B (en) | 2023-06-23 |
Family
ID=83583148
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210895095.2A Active CN115193812B (en) | 2022-07-28 | 2022-07-28 | Cleaning station system of chemiluminescent detector |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115193812B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115932310A (en) * | 2022-12-27 | 2023-04-07 | 山东中鸿特检生物科技有限公司 | Detachable sample site and chemiluminescence immunoassay device |
| CN117333484A (en) * | 2023-11-30 | 2024-01-02 | 山东罗斯夫新材料科技有限公司 | Method for detecting acrylic emulsion production wastewater based on image processing |
Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103599898A (en) * | 2013-10-29 | 2014-02-26 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer magnetic bead washing device |
| WO2015025378A1 (en) * | 2013-08-21 | 2015-02-26 | 株式会社日立製作所 | Sample analysis method and sample analysis device |
| CN104722526A (en) * | 2015-03-20 | 2015-06-24 | 北京中航赛维生物科技有限公司 | Magnetism separating device |
| CN109030807A (en) * | 2018-08-09 | 2018-12-18 | 苏州长光华医生物医学工程有限公司 | The detection system and method for needle are blocked up suitable for cleaning separation function |
| CN211757232U (en) * | 2019-12-03 | 2020-10-27 | 北京乐普诊断科技股份有限公司 | Magnetic bead cleaning device for analyzer |
| CN213364798U (en) * | 2019-10-25 | 2021-06-04 | 苏州长光华医生物医学工程有限公司 | Waste liquid needle detection device for cleaning and separating waste liquid of chemiluminescence analyzer |
| CN113514655A (en) * | 2021-05-13 | 2021-10-19 | 桂林优利特医疗电子有限公司 | Small-size full-automatic chemiluminescence immunoassay appearance liquid route system |
| CN215415060U (en) * | 2021-07-01 | 2022-01-04 | 瑞捷生物科技江苏有限公司 | Cleaning disc for chemiluminescence analyzer |
| CN113916780A (en) * | 2021-10-08 | 2022-01-11 | 安图实验仪器(郑州)有限公司 | Magnetic bead cleaning, incubation and photometric device |
| US20220397583A1 (en) * | 2020-01-12 | 2022-12-15 | Lansion Biotechnology Co., Ltd. | Fully-automatic chemiluminescence immunoassay analyzer |
-
2022
- 2022-07-28 CN CN202210895095.2A patent/CN115193812B/en active Active
Patent Citations (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2015025378A1 (en) * | 2013-08-21 | 2015-02-26 | 株式会社日立製作所 | Sample analysis method and sample analysis device |
| CN103599898A (en) * | 2013-10-29 | 2014-02-26 | 北京利德曼生化股份有限公司 | Full-automatic chemiluminescence immune analyzer magnetic bead washing device |
| CN104722526A (en) * | 2015-03-20 | 2015-06-24 | 北京中航赛维生物科技有限公司 | Magnetism separating device |
| CN109030807A (en) * | 2018-08-09 | 2018-12-18 | 苏州长光华医生物医学工程有限公司 | The detection system and method for needle are blocked up suitable for cleaning separation function |
| CN213364798U (en) * | 2019-10-25 | 2021-06-04 | 苏州长光华医生物医学工程有限公司 | Waste liquid needle detection device for cleaning and separating waste liquid of chemiluminescence analyzer |
| CN211757232U (en) * | 2019-12-03 | 2020-10-27 | 北京乐普诊断科技股份有限公司 | Magnetic bead cleaning device for analyzer |
| US20220397583A1 (en) * | 2020-01-12 | 2022-12-15 | Lansion Biotechnology Co., Ltd. | Fully-automatic chemiluminescence immunoassay analyzer |
| CN113514655A (en) * | 2021-05-13 | 2021-10-19 | 桂林优利特医疗电子有限公司 | Small-size full-automatic chemiluminescence immunoassay appearance liquid route system |
| CN215415060U (en) * | 2021-07-01 | 2022-01-04 | 瑞捷生物科技江苏有限公司 | Cleaning disc for chemiluminescence analyzer |
| CN113916780A (en) * | 2021-10-08 | 2022-01-11 | 安图实验仪器(郑州)有限公司 | Magnetic bead cleaning, incubation and photometric device |
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115932310A (en) * | 2022-12-27 | 2023-04-07 | 山东中鸿特检生物科技有限公司 | Detachable sample site and chemiluminescence immunoassay device |
| CN115932310B (en) * | 2022-12-27 | 2023-08-18 | 山东中鸿特检生物科技有限公司 | Detachable sample position and chemiluminescent immunoassay device |
| CN117333484A (en) * | 2023-11-30 | 2024-01-02 | 山东罗斯夫新材料科技有限公司 | Method for detecting acrylic emulsion production wastewater based on image processing |
| CN117333484B (en) * | 2023-11-30 | 2024-02-20 | 山东罗斯夫新材料科技有限公司 | Method for detecting acrylic emulsion production wastewater based on image processing |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115193812B (en) | 2023-06-23 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN115193812A (en) | Cleaning station system of chemiluminescence detector | |
| US11300571B2 (en) | Assay apparatuses, methods and reagents | |
| EP1870713B1 (en) | Apparatus for multiple automatic analysis of biosamples, method for autoanalysis, and reaction cuvette | |
| KR101725340B1 (en) | Assay apparatuses, methods and reagents | |
| CN104897901A (en) | Goldmag particle-based acridinium ester chemiluminescence immunological detection method of HE4 | |
| US20080206099A1 (en) | Magnetic separator and analyzer using the same | |
| WO2020062946A1 (en) | Magnetic bead cleaning device and chemiluminescence immunoassay analyzer | |
| WO2021031776A1 (en) | Fully-automated chemiluminescence immunoassay method | |
| JP5331551B2 (en) | Analysis equipment | |
| AU2023258454A1 (en) | Assay apparatuses, methods and reagents | |
| CN110082548B (en) | Adsorption mechanism, cleaning device, chemiluminescence detector and cleaning method | |
| CN110076154B (en) | Reaction cup cleaning method of rotating disc type reaction cup cleaning device | |
| JPH09325148A (en) | Chemical analyzer | |
| CN114965280B (en) | Reaction cup magnetic bead separation module of chemiluminescence detector | |
| CN208140724U (en) | Automatic lmunoassays analyzer | |
| CN213517172U (en) | Full-automatic chemiluminescence immunoassay analyzer | |
| CN209148549U (en) | Chemiluminescence detecting | |
| CN110082291B (en) | Adsorption mechanism, cleaning device, chemiluminescence detector and cleaning method | |
| CN104181140A (en) | Detection disc applied to full-automatic tubular time resolution detector | |
| CN214895325U (en) | Homogeneous immunoassay's instant detecting system | |
| CN111381056A (en) | Magnetic separation device, sample analyzer, and flow type fluorescence immunoassay analyzer | |
| CN103454135A (en) | Device for combining sample enrichment with analytical instrument | |
| AU2018204596B2 (en) | Assay Apparatuses, Methods and Reagents | |
| CN210534169U (en) | Sample device for full-automatic chemiluminescence immunoassay | |
| KR102378634B1 (en) | Rapid immunodiagnostic method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |