CN115918910A - Probiotics protein multivitamin and preparation method thereof - Google Patents

Probiotics protein multivitamin and preparation method thereof Download PDF

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CN115918910A
CN115918910A CN202310037042.1A CN202310037042A CN115918910A CN 115918910 A CN115918910 A CN 115918910A CN 202310037042 A CN202310037042 A CN 202310037042A CN 115918910 A CN115918910 A CN 115918910A
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protein
probiotic
multivitamin
lactobacillus
immune
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邱德文
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Changsha Zhongweike Bioengineering Co ltd
Xiangtan Zhongweike Bioengineering Co ltd
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Changsha Zhongweike Bioengineering Co ltd
Xiangtan Zhongweike Bioengineering Co ltd
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Abstract

The invention belongs to the field of probiotic compound vitamins, and particularly discloses a probiotic protein compound vitamin and a preparation method thereof, wherein the probiotic protein compound vitamin contains an immune protein activating factor and a compound vitamin; the immune protein activator contains probiotic proteins: the probiotic is selected from: one or more of lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, animal bifidobacterium animalis, bifidobacterium lactis and lactococcus lactis subspecies lactis; the invention extracts protein in probiotics capable of improving human immunity and strengthening physique, adds multivitamins beneficial to strengthening human immunity and promoting fatigue recovery through scientific compatibility according to the principle of function complementation, and finally adds high-activity components such as maca powder, pseudo-ginseng powder and the like according to the edible taste and the edible convenience to prepare the probiotic protein multivitamin preparation.

Description

Probiotics protein compound vitamin and preparation method thereof
Technical Field
The invention belongs to the field of probiotic multivitamins, and particularly discloses a probiotic protein multivitamin and a preparation method thereof.
Background
Probiotics are active microorganisms that, when ingested in sufficient quantities, can produce health benefits. Probiotics that maintain adequate levels in the body have been shown to support immune function, aid digestive health, and even promote metabolism. The two most well known probiotics that support digestive and immune health are bifidobacteria, most commonly found in the large intestine, while lactobacilli are present in the small intestine. The world health organization defines probiotics as "active microorganisms that, when ingested in sufficient quantities, produce health benefits to the host". Many foods and supplements contain active microorganisms, but only those containing characteristic strains with scientifically proven effects on health can be correctly called "probiotics". According to this definition, probiotic strains must be characterized by their specific properties, efficacy and health benefits. Probiotics are a class of active microorganisms that act by improving the balance of the host's intestinal microflora, and can produce a definite health effect to improve the host's microecological balance and exert beneficial effects. In 2001, the world Food and Agriculture Organization (FAO) and the World Health Organization (WHO) also defined probiotics as follows: live bacteria which exert an effective effect on the health of the consumer when ingested in an appropriate amount. The main bacteria or fungi in human bodies and animal bodies are: clostridium butyricum, lactobacillus, bifidobacterium, lactobacillus acidophilus, actinomycetes, saccharomycetes and the like. The probiotics are closely related to the health and longevity of human beings, delicious foods which are eaten by people for thousands of years, such as yoghourt, pickled vegetables, pickled Chinese cabbage, fermented soya beans, natto, black tea fungus and the like, are probiotic foods, and beneficial microorganisms in the foods, such as yeast, bifidobacterium, lactobacillus, actinomycetes, bacillus subtilis and the like, bring the delicious taste and health to people. The intestinal flora is the largest bacterial bank and the largest micro-ecosystem of human beings, inhabits more than 1000 kinds of bacteria, 100 trillion bacteria, and is 10 times of the total number of human cells, and the intestinal flora comprises various microorganisms such as bacteria, fungi, archaea, protozoa and plants (even viruses). The intestinal flora is closely related to the human body, is a unity of mutualistic relationship, and is also called as the second genome of human.
Probiotics (English: probiotics) refers to an ingesting microorganism that is generally considered to be positively beneficial to a host (e.g., an animal or human) after ingestion. Medically, scientific evidence currently fails to show health support effects of probiotic use in improving the immune system, improving gut health, relieving constipation, and preventing colds, and the federal trade commission in the united states also does not allow for such advertising of claim efficacy [6]. The british national health care agency also does not consider sufficient evidence for these alleged therapeutic effects. Probiotics are generally safe, but in very few cases may cause bacterial-host interactions and adverse reactions. It is believed by researchers that the administration of probiotics after antibiotic treatment would prevent the recovery of the gut microbiome.
The earliest probiotic for human consumption was from yogurt. As early as more than 3000 years before the west yuan, buttermilk has been produced and drunk by ancient nomadic nationalities living in turkish plateau. The initial yogurt milk may have originated by chance. After the storage method is lagged, the goat milk is often deteriorated due to the pollution of harmful bacteria to the goat milk, but lactic acid bacteria of the yogurt accidentally enter the goat milk in the primary air, and the beneficial bacteria cause the goat milk to change similarly to the change in the gastrointestinal tract, so that the goat milk becomes more sour, sweet and palatable, which is the earliest yogurt. Early 20 th century, russian scientist Ilia Mei Nikov (
Figure BDA0004049183150000021
Metchnikoff) in studying why people who have a long life are more in bulgaria, it was found that these people all like to drink yogurt, and a theory that lactic acid bacteria contained in fermented milk are beneficial to human health was proposed. The result of the Mei-Nikov study was inspired by the Isaac Carasso (English) of Spanish businessman to establish a yogurt manufacturing plant (today's Daneng group) in 1919, and the yogurt was sold in the pharmacy as a "long-life beverage" with medicinal effect.
The term "lactic acid bacteria" is a general term for bacteria capable of producing large amounts of lactic acid by fermentation using carbohydrates, and is a rather bulky group of bacteria, and recently, through the development of science and molecular biology, it is widely recognized that lactic acid bacteria are subdivided and expanded from the early 4 genera (genus) to the 17 genera of 273 (species): enterococcus (Enterococcus), lactobacillus (Lactobacillus), bacillus (Bacillus), clostridium (Clostridium), lactococcus (Lactococcus lactis), leuconostoc (english: leuconostoc) (Leuconostoc), pediococcus (english: pediococcus (Pediococcus), carnobacterium (Carnobacterium), prokaryotum (Vagococcus), tetragenococcus (English), bifidobacterium (English), atopobium (Atopobium), weissella (Weissella), anaerococcus (Abiotrophia), streptococcus (English), rhodomela (Salmonella), oenococcus (Oenococcus), paralactis (Paralactis-Lactobacillus), lactobacillus (Paralactis-Lactobacillus). It mainly colonizes the small intestine, where the greatest number of bacteria are located, and it trails all the vaginal wall, cervix and urethra. Can slightly reduce cholesterol (4%), is suspected to regulate immunity and reduce infection of candida vaginitis and the like. Lactobacillus brevis (English: lactobacillus brevis) -Lactobacillus brevis, the existence of which can be found in human mouth, mouse tail and intestinal tract, and also in milk and cheese. Can inhibit intestinal pathogenic bacteria, maintain digestive tract function, promote appetite and regulate immunity. Lactobacillus casei-Lactobacillus casei, abbreviated as C. Is quite acid-resistant, can effectively pass the test of choline in gastric acid, and can enter the intestinal tract to colonize on a large scale. L.delbrueckii subsp.bulbgaricus (English: lactobacillus delbrueckii subsp.bulbgaricus) -Lactobacillus bulgaricus is a subspecies of Lactobacillus delbrueckii and is widely used in the process of making yogurt. Is suspected to have the effects of regulating the health of the gastrointestinal tract, promoting the digestion and absorption, enhancing the immunologic function and the like. Lactobacillus johnsonii, abbreviated as LJ bacterium. Lactobacillus paracasei, LP for short. Has good effects of resisting gastric acid and bile salt and colonizing in intestinal tract. Can promote the secretion of Th1 cell hormone in vivo, inhibit the sensitive immune response caused by Th2 cell, and balance the immune system. Can be used for treating allergic symptoms such as atopic dermatitis. Lactobacillus reuteri (English: lactobacillus reuteri) -Lactobacillus delbrueckii, R bacteria for short. Is one of the few lactic acid bacteria found in adults and infants, and can help the intestinal cells of babies to grow and promote the reproduction of beneficial bacteria. Lactobacillus rhamnous (English: lactobacillus rhamnous) -Lactobacillus rhamnosus. Lactobacillus rhamnosus GG-LGG bacteria for short. Formally named lactobacillus rhamnosus GG strain. Is the most studied probiotic bacteria in the world at present and is one of the first probiotics which are proved to be capable of surviving and colonizing the intestinal tract of human body. Can increase sufficient intercellular albumin-10 in serum and reduce the formation of cytokines that cause local sensitive immune responses. Can promote growth of beneficial bacteria, reduce allergy to milk or food, and treat unknown reasons or acute diarrhea. Lactobacillus salivarius-Lactobacillus salivarius. Bifidobacterium bifidum-bifidum, called B bacteria for short. The intestinal acid environment is maintained, and the propagation of harmful bacteria is reduced; it has effects in enhancing immunity, reducing cholesterol, improving constipation, and regulating intestinal tract physiological function. Bifidobacterium infantis-infant type Bifidenbacilia. It is known as bifida infantum because it is found in the intestine of infants. Can relieve inflammatory injury caused by excessive reaction of human immune system. Bifidobacterium lactis-Ratti B. Is obtained by separating from the feces of healthy infants and is one of the most common flora in human intestinal tract. Resists gastric acid and bile salt, colonizes in intestinal tracts and achieves the effect of increasing good bacteria in vivo. Can relieve gastritis and improve lactose intolerance. Bifidobacterium longum, also called "Rhizopus longus". The beneficial bacteria in the intestinal tract are mainly bifidobacterium longum and are also true human protozoan strains. Can effectively regulate Th2 cell immunoreaction, reduce IgE content in blood of allergic patients, possibly improve allergic symptoms caused by pollen, and possibly improve diarrhea caused by antibiotics and food pathogenic bacteria. Bacillus coagulons, a strain of Lactobacillus sp. Can be used for improving symptoms caused by intestinal dysbacteriosis, such as acute and chronic diarrhea, chronic constipation, abdominal distention, dyspepsia, etc. Clostridium clostridium butyricum, also known as clostridium butyricum, butyric acid bacteria or uterine fungus. Preventing and treating chronic diarrhea, antibiotic-associated enteritis, constipation or alternate diarrhea and constipation, and normalizing intestinal function. Saccharomyces boulardii-Saccharomyces boulardii.
According to a recently published '2021 intestinal health investigation report', the intestinal problems of Chinese people tend to be popular, people who often sit in offices are high-risk people, constipation symptoms caused by the intestinal problems are the most common intestinal problems, and constipation often causes other problems such as oral peculiar smell, abdominal distension, dyspepsia and the like. The intestinal tract is not only the digestive organ of the human body, but also the largest immune and toxin expelling organ of the human body. The immune cells are distributed on the intestinal canal wall, and can resist a large amount of harmful substances outside the human body to damage the health of the human body, so that the human body is prevented from being injured, and the intestinal canal wall is called as a first defense line for human health. Maintaining intestinal health plays a crucial role in overall physical health. According to the statistics of the world health organization, the number of people dying from gastrointestinal diseases every year in the world is more than 1000 ten thousand, and one fifth of people in China have gastrointestinal diseases and live at the first position in the world. Meanwhile, when diseases occur, people often urgently find an effective solution, but the prevention is still the optimal choice in the past from the present point of view.
Disclosure of Invention
In order to solve the problems, the invention discloses a probiotic protein multivitamin and a preparation method thereof, wherein proteins in probiotics capable of improving human immunity and enhancing physique are extracted, multivitamins beneficial to enhancing human immunity resistance and promoting fatigue recovery are added through scientific compatibility according to the principle of function complementation, and finally, high-active components such as maca powder and pseudo-ginseng powder are added according to edible mouthfeel and edible convenience to prepare probiotic protein multivitamin granules, tablets, buccal tablets, capsules and oral liquid.
The technical scheme of the invention is as follows:
a probiotic protein compound vitamin contains immune protein activating factor and compound vitamin; the immune protein activator contains probiotic proteins:
the probiotic is selected from: one or more of lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, animal bifidobacterium animalis, bifidobacterium lactis and lactococcus lactis subspecies lactis.
Further, the above-mentioned probiotic protein multivitamin, the immune protein activator is prepared by the following steps: the probiotic strains are fermented to obtain a large amount of thalli, and then the immune protein activator with the immune activation function in the probiotic strains is prepared by shearing and crushing cells, centrifugal precipitation and fractional separation and extraction.
Furthermore, the fermentation process of the probiotic protein multivitamin is that a plurality of strains are fermented simultaneously,
the formula of the culture medium is as follows: 1.4-2.3% of sweet corn flour, 0.8-1.5% of tryptone, 0.7-1.2% of beef extract, 0.3-1% of yeast extract, 0.4-0.8% of glucose, 0.3-0.6% of sodium glutamate, 0.3-0.6% of white granulated sugar, 0.05-0.15% of potassium dihydrogen phosphate, 0.02-0.05% of ferrous sulfate and the balance of water;
the fermentation parameters are as follows: pH value: 6.0 to 6.5; and (3) sterilization temperature: 115-121 ℃; fermentation temperature: 35-40 ℃; the fermentation time is 28-35h.
Furthermore, the probiotic protein multivitamin contains effective components and auxiliary materials,
the effective components are as follows: immune protein activating factor, compound vitamin, maca powder and pseudo-ginseng powder;
the auxiliary materials are as follows: dextrin and corn starch.
Further, the probiotic protein multivitamin contains one or more of vitamins A, B, C, D, E and K.
Vitamin complex: vitamins are a class of organic compounds essential to maintain human health, promote growth and development, and regulate physiological functions. These compounds are present in natural foods and are generally not synthesized or synthesized in small amounts in vivo, and are not satisfactory. Due to dietary habits, lack of nutrition knowledge and changes of life styles of modern people, an ideal balanced diet mode is difficult to achieve, according to investigation of authoritative departments, most people in China can not provide certain vitamins and mineral substances in a balanced way in daily diet, calcium, iron, vitamin A and B2 are common, and zinc, selenium, vitamin B1 and vitamin C are common. It is therefore necessary to administer nutrient supplements. In the case of malnutrition, multivitamins are often lacking and multiple vitamins should be supplemented, since supplementation of only one if multiple nutrients are lacking interferes with the absorption and metabolism of other vitamins and minerals.
Vitamin A: promoting growth, and maintaining normal functions of epithelial tissue such as mucosa, conjunctiva, cornea, etc.; participate in the synthesis of rhodopsin, and enhance the photosensitivity of the retina; participate in many oxidative processes in the body. Vitamin B2 (also known as riboflavin) deficiency affects the biological oxidation of the body and causes metabolic disorders. Manifested by angular cheilitis, glossitis, conjunctivitis, scrotitis, etc. Vitamin C (also known as vitamin C, ascorbic acid) is involved in the redox process and carbohydrate metabolism process in vivo. Participating in the generation of interstitial substance of tissue cells, reducing the fragility of capillary vessels, accelerating the blood coagulation and increasing the resistance to infection. Participate in the detoxification function. It can promote absorption of iron in intestinal tract, and has antihistaminic (antiallergic) effect. Vitamin D2 (alternative: calciferol) vitamin D3 alternative: cholecalciferol: promote the absorption and storage of calcium and phosphorus in the intestine, regulate the calcium and phosphorus levels in the blood plasma by matching with parathyroid hormone and calcitonin, and promote the normal calcification of bones. Vitamin E: a strong antioxidant; can protect the biological membrane from being attacked by free radicals and is an effective anti-aging nutrient. Improving the immunity of the human body. The integrity of the red blood cells is maintained, and the biosynthesis of the red blood cells is promoted. Vitamin E is lipid soluble and its toxicity should be noted. When the medicine is taken by 800-3200 mg/day, muscle weakness, fatigue, vomiting and diarrhea can occur occasionally. The most significant toxic effects of vitamin E >1000 mg/day are the significant bleeding that can result.
The invention selects high-quality vitamin A, B, C, D, E, K and other compound vitamins as the effective components, and can further enhance the immunity of the human body, promote the metabolism of the human body, improve the physique of the human body and the like.
Further, the probiotic protein multivitamin is prepared from the following raw materials in percentage by mass: 80-90% of immune protein activating factor, 2-4% of compound vitamin, 0.5-2% of maca powder, 0.5-2% of pseudo-ginseng powder, 3-8% of dextrin and 2-6% of corn starch.
Preferably, the probiotic protein multivitamin is prepared from the following raw materials in percentage by mass: 85% of immune protein activating factor, 3% of compound vitamin, 1% of maca powder, 1% of pseudo-ginseng powder, 5% of dextrin and 5% of corn starch.
Furthermore, the dosage form of the probiotic protein multivitamin comprises but is not limited to oral liquid, granules, tablets, buccal tablets or capsules.
Furthermore, in the granule, the fineness of the granule of the probiotic protein multivitamin granule is 10-50 meshes, but the fineness of the granule which can not pass through 10 meshes and can pass through 65 meshes is not more than 8%; the finished product has dry appearance, uniform particles and consistent color, and has no agglomeration and moisture; the water solution electuary can be dissolved by adding 20 parts of boiled water into 1 part of finished product; the granule has good taste, small size, and convenient administration, storage and transportation.
Further, the preparation method of the probiotic protein multivitamin comprises the following steps:
s1, performing shake flask fermentation on strains, performing fermentation in an S2 fermentation tank, breaking cell walls in S3, performing centrifugal filtration and precipitation in S4, adding multivitamin, maca powder, pseudo-ginseng powder and auxiliary materials in S5, fully stirring and mixing, and S6, performing spray drying and granulation or tabletting.
Compared with the prior art, the invention has the following beneficial effects:
in the technical scheme, the probiotics are optimized and combined to form a compound microbial flora, a stable probiotic flora is formed through activation, culture medium selection and fermentation technical parameter optimization, fermentation culture is carried out, and the immune protein activating factor, namely the probiotic protein, is obtained through separation by the steps of cell shearing and crushing, centrifugal precipitation separation and the like of a microbial fermentation culture solution. The obtained probiotic protein has multiple characteristics of probiotics, improves the immunity of a human body, promotes the metabolism of the human body, promotes the metabolic circulation of the human body, is not a live bacterium, can avoid negative effects such as intestinal flora imbalance and the like caused by the fact that the live bacterium is not fused with the intestinal flora, can participate in and promote the metabolic pathway of the immunity of the human body, activates an immune signal pathway of the human body, and meanwhile, can promote the metabolism of the human body, improves the nutrient absorption and is beneficial to the health of the human body. The protein produced by the probiotics has strong stability and definite action target, can directly participate in the immune and metabolic pathways of human bodies, and can directly activate the immune signal transduction of the human bodies and stimulate the generation of immune metabolism, thereby activating the immune metabolic reaction of the human bodies and avoiding the limitation of viable bacteria. The probiotic protein has the advantages of strong stability, clear target, quick activation, easy absorption and the like, and has wide application prospect. The oral liquid, the tablet, the buccal agent and the capsule of the probiotic protein multivitamin are prepared according to the national relevant standards. The oral liquid, the tablet, the buccal agent and the capsule of the probiotics protein compound vitamin are all in the protection scope of the invention. The probiotic protein has the functions of activating the immune metabolic pathway of a human body, promoting the immunity and metabolic function of the human body and enhancing the immunity of the human body, and the addition of the multivitamin in the probiotic protein is more beneficial to the improvement of the immunity of the human body and the enhancement of the metabolism of the human body.
The invention extracts protein in probiotics capable of improving human immunity and strengthening physique, adds multivitamins beneficial to strengthening human immunity and promoting fatigue recovery through scientific compatibility according to the principle of function complementation, and finally adds high-active components such as maca powder, pseudo-ginseng powder and the like according to the edible taste and the edible convenience to prepare probiotics protein multivitamin granules, tablets, buccal tablets, capsules and oral liquid or tablets. The product is mainly prepared by combining immune protein repair activating factors, compound vitamins, essential amino acids of human bodies and the like, has wide application prospect, and has the main functions of: the health-care food has the advantages of promoting human body metabolism, improving human body immune potential, enhancing human body immunocompetence, facilitating fatigue recovery and quick restoration of physical fitness after diseases, having good spirit and appearance and vigorous energy, enhancing the physical fitness of human bodies after being used, and having certain resistance to common diseases.
Drawings
FIG. 1 is a schematic view of the preparation process of the present invention.
Detailed Description
The probiotic protein multivitamin is prepared from the following raw materials in percentage by mass: 80-90% of immune protein activating factor, 2-4% of compound vitamin, 0.5-2% of maca powder, 0.5-2% of pseudo-ginseng powder, 3-8% of dextrin and 2-6% of corn starch;
the immune protein activating factor is prepared by the following steps: fermenting probiotic strains to obtain a large number of thalli, shearing and crushing cells, centrifuging and precipitating, and separating and extracting step by step to obtain immune protein activators with immune activation function;
the probiotics comprise: lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, bifidobacterium animalis subsp, bifidobacterium lactis, and lactococcus lactis subsp lactis;
the fermentation process is that a plurality of strains are fermented simultaneously,
the formula of the culture medium is as follows: 1.4-2.3% of sweet corn flour, 0.8-1.5% of tryptone, 0.7-1.2% of beef extract, 0.3-1% of yeast extract, 0.4-0.8% of glucose, 0.3-0.6% of sodium glutamate, 0.3-0.6% of white granulated sugar, 0.05-0.15% of potassium dihydrogen phosphate, 0.02-0.05% of ferrous sulfate and the balance of water;
the fermentation parameters are as follows: pH value: 6.0-6.5; and (3) sterilization temperature: 115-121 ℃; fermentation temperature: 35-40 ℃; the fermentation time is 28-35h;
the compound vitamin contains vitamins A, B, C, D, E and K.
The preparation method of the probiotic protein multivitamin comprises the following steps of:
s1, performing shake flask fermentation on strains, performing fermentation in an S2 fermentation tank, breaking cell walls in S3, performing centrifugal filtration and precipitation in S4, adding multivitamin, maca powder, pseudo-ginseng powder and auxiliary materials in S5, fully stirring and mixing, and S6, performing spray drying and granulation or tabletting.
The technical solutions in the embodiments of the present invention are clearly and completely described below, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The strain numbers of the probiotics in the examples of the present invention are as follows: lactobacillus acidophilus (strain No.: NCFM), lactobacillus rhamnosus (strain No.: LGG and HN 001), lactobacillus reuteri (strain No.: DSM 17938), bifidobacterium breve (strain No.: M-16 v), lactobacillus fermentum (strain No.: CECT 5716), bacillus subtilis (strain No.: 1.14985), bifidobacterium animalis subspecies animal (strain No.: BB-12), bifidobacterium lactis (strain No.: HN019 and Bi-07), lactococcus lactis subspecies lactis (strain No.: 1.2472).
Example 1
The probiotic protein multivitamin is prepared from the following raw materials in percentage by mass: 80% of immune protein activating factor, 3% of compound vitamin, 1% of maca powder, 2% of pseudo-ginseng powder, 8% of dextrin and 6% of corn starch;
the immune protein activating factor is prepared by the following steps: fermenting probiotic strains to obtain a large amount of thalli, shearing and crushing cells, centrifuging and precipitating, and separating and extracting step by step to obtain immune protein activators with immune activation effect;
the probiotics comprise: lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, bifidobacterium animalis subsp, bifidobacterium lactis, and lactococcus lactis subsp lactis;
the fermentation process is that a plurality of strains are fermented simultaneously,
the formula of the culture medium is as follows: 1.4% of sweet corn flour, 0.8% of tryptone, 0.7% of beef extract, 0.3% of yeast extract, 0.4% of glucose, 0.3% of sodium glutamate, 0.3% of white granulated sugar, 0.05% of potassium dihydrogen phosphate, 0.02% of ferrous sulfate and the balance of water;
the fermentation parameters are as follows: pH value: 6.0-6.5; and (3) sterilization temperature: 115-121 ℃; fermentation temperature: 35-40 ℃; the fermentation time is 28-35h;
the compound vitamin contains vitamins A, B, C, D, E and K.
The preparation method of the probiotic protein multivitamin mainly comprises the following steps with reference to the flow of figure 1:
s1, performing shake flask fermentation on strains, performing fermentation in an S2 fermentation tank, breaking cell walls in S3, performing centrifugal filtration and precipitation in S4, adding multivitamin, maca powder, pseudo-ginseng powder and auxiliary materials in S5, fully stirring and mixing, and S6, performing spray drying and granulation or tabletting.
Example 2
The probiotic protein multivitamin is prepared from the following raw materials in percentage by mass: 85% of immune protein activating factor, 3% of compound vitamin, 1.5% of maca powder, 1.5% of pseudo-ginseng powder, 5% of dextrin and 4% of corn starch;
the immune protein activator is prepared by the following steps: fermenting probiotic strains to obtain a large amount of thalli, shearing and crushing cells, centrifuging and precipitating, and separating and extracting step by step to obtain immune protein activators with immune activation effect;
the probiotics comprise: lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, bifidobacterium animalis subsp, bifidobacterium lactis, and lactococcus lactis subsp lactis;
the fermentation process is that a plurality of strains are fermented simultaneously,
the formula of the culture medium is as follows: 1.8% of sweet corn flour, 1.3% of tryptone, 1.0% of beef extract, 0.7% of yeast extract, 0.7% of glucose, 0.45% of sodium glutamate, 0.45% of white granulated sugar, 0.1% of potassium dihydrogen phosphate, 0.03% of ferrous sulfate and the balance of water;
the fermentation parameters are as follows: pH value: 6.0-6.5; and (3) sterilization temperature: 115-121 ℃; fermentation temperature: 35-40 ℃; the fermentation time is 28-35h;
the compound vitamin contains vitamins A, B, C, D, E and K.
The preparation method of the probiotic protein multivitamin mainly comprises the following steps with reference to the flow of figure 1:
s1, performing shake flask fermentation on strains, performing fermentation in an S2 fermentation tank, breaking cell walls in S3, performing centrifugal filtration and precipitation in S4, adding multivitamin, maca powder, pseudo-ginseng powder and auxiliary materials in S5, fully stirring and mixing, and S6, performing spray drying and granulation or tabletting.
Example 3
The probiotic protein multivitamin is prepared from the following raw materials in percentage by mass: 90% of immune protein activating factor, 2% of compound vitamin, 1% of maca powder, 0.5% of pseudo-ginseng powder, 3% of dextrin and 3.5% of corn starch;
the immune protein activating factor is prepared by the following steps: fermenting probiotic strains to obtain a large number of thalli, shearing and crushing cells, centrifuging and precipitating, and separating and extracting step by step to obtain immune protein activators with immune activation function;
the probiotics comprise: lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, bifidobacterium animalis subsp, bifidobacterium lactis, and lactococcus lactis subsp lactis;
the fermentation process is that a plurality of strains are fermented simultaneously,
the formula of the culture medium is as follows: 2.3% of sweet corn flour, 1.5% of tryptone, 1.2% of beef extract, 1% of yeast extract, 0.8% of glucose, 0.6% of sodium glutamate, 0.6% of white granulated sugar, 0.15% of potassium dihydrogen phosphate, 0.05% of ferrous sulfate and the balance of water;
the fermentation parameters are as follows: pH value: 6.0-6.5; and (3) sterilization temperature: 115-121 ℃; fermentation temperature: 35-40 ℃; the fermentation time is 28-35h;
the compound vitamin contains vitamins A, B, C, D, E and K.
The preparation method of the probiotic protein multivitamin mainly comprises the following steps with reference to the flow of figure 1:
s1, performing shake flask fermentation on strains, performing fermentation in an S2 fermentation tank, breaking cell walls in S3, performing centrifugal filtration and precipitation in S4, adding multivitamin, maca powder, pseudo-ginseng powder and auxiliary materials in S5, fully stirring and mixing, and S6, performing spray drying and granulation or tabletting.
Experimental example 1
Growth test by mouse feeding
1. The test animals were: SPF grade ICR mice, 40, half male and female, weight 18.0-22.0 g when tested. All animals were acclimatized for 5 days before testing, and had free access to water and food. The experimental animals are raised in a barrier environment with the temperature of 20-26 ℃ and the relative humidity of 40-70%.
2. Grouping tests: every 10 female mice 10 male mice were grouped into two groups. The stomach was perfused with purified water as a blank group, and the probiotic protein multivitamin perfusate prepared in example 1 was perfused with purified water as a treatment group.
3. Subject administration mode: animals are fasted for 16h before the test, and are gavaged once daily at the rate of 10mL/kg. Bw, and are continuously gavaged for 7d.
4. The test method comprises the following steps: two groups of mice were weighed for food intake: feeding 100g/d of maintenance feed to each group in a fixed amount, and weighing the residual feed in the previous day; control mice weight change: mice were weighed on day eight of the experiment.
5. As a result: the test results are shown in table 1 below. The food intake of the mice in the test group is increased by 5.33 percent compared with the control group, and the body weight is increased by 6.90 percent compared with the control group after the test is finished.
TABLE 1 mouse feed growth test
Blank group Treatment group Treatment group was increased (%)
Daily food intake (g) 50.6 53.3 5.33
Average body weight (g) 23.2 24.8 6.90
As can be seen from the data in the table 1, the probiotic protein multivitamin disclosed by the invention can remarkably promote the appetite of mice and increase the weight of the mice, and the product has high nutritional value.
Experimental example 2
Female, 85 years old, 41.20 kg, because of unwilling to wear false teeth, the diet is basically liquid diet or soft glutinous food, which causes slow gastrointestinal motility, constipation, defecation for 1-2 weeks, and dry excrement in small granule shape. Under the condition of no change of dietary structure, the product (2 g per day in example 1) is taken for three weeks, then the weight is 42.10 kg, the defecation is carried out for 3-4 times in one week, the defecation is changed into soft stool, and the complexion and mental state are obviously improved.
Experimental example 3
The male is 45 years old, 78.15 kilograms, and is sensitive to intestines and stomach and frequent diarrhea due to long-term drinking and irregular diet, and the average diarrhea is 1-2 times per week. The product (product prepared in example 2, 2g daily) was taken on a consistent basis with no change in dietary structure, and the weight was 77.10 kg two months later, with 3 diarrhea days for two months, both occurring in the first two weeks of use.
Experimental example 4
For men, the weight of 66.35 kg in 24 years old is changed from normal night stay, overeating, being loved to roast, causing gastrointestinal dysfunction at night and constipation and diarrhea, and under the condition of not changing the dietary structure, the body weight of 64.20 kg after the product (the product prepared in example 3, 1g per day) is taken continuously for three months, the normal functions of the intestines and the stomach are basically recovered, and the times of constipation and diarrhea are reduced.
From the above experimental examples it can be seen that: the probiotic protein has the functions of activating the immune metabolic pathway of a human body, promoting the immunity and metabolic function of the human body and enhancing the immune physique of the human body, and the addition of the multivitamins in the probiotic protein is more favorable for improving the immunity of the human body and enhancing the metabolism of the human body, is favorable for fatigue recovery and quick repair of the physique after illness.
The above examples are a limited number of preferred embodiments of the present invention, and the description thereof is more specific and detailed, but should not be construed as limiting the scope of the present invention. It should be noted that, for a person skilled in the art, several variations and modifications can be made without departing from the inventive concept, which falls within the scope of the present invention.

Claims (10)

1. A probiotic protein multivitamin, which is characterized by comprising an immune protein activating factor and multivitamin; the immune protein activator contains probiotic proteins:
the probiotic is selected from: one or more of lactobacillus acidophilus, lactobacillus rhamnosus, lactobacillus reuteri, bifidobacterium breve, lactobacillus fermentum, bacillus subtilis, animal species of bifidobacterium animalis, bifidobacterium lactis and lactococcus lactis.
2. The probiotic protein multivitamin of claim 1, wherein the immune protein activator is prepared by the following steps: the probiotic strains are fermented to obtain a large number of thalli, and then the immunoprotein activator with the immune activation function in the probiotic strains is prepared by shearing and crushing cells, centrifugal precipitation and step-by-step separation and extraction.
3. The probiotic protein multivitamin of claim 2, wherein the fermentation process is simultaneous fermentation of multiple strains,
the formula of the culture medium is as follows: 1.4-2.3% of sweet corn flour, 0.8-1.5% of tryptone, 0.7-1.2% of beef extract, 0.3-1% of yeast extract, 0.4-0.8% of glucose, 0.3-0.6% of sodium glutamate, 0.3-0.6% of white granulated sugar, 0.05-0.15% of potassium dihydrogen phosphate, 0.02-0.05% of ferrous sulfate and the balance of water;
the fermentation parameters are as follows: pH value: 6.0-6.5; sterilization temperature: 115-121 ℃; fermentation temperature: 35-40 ℃; the fermentation time is 28-35h.
4. The probiotic protein multivitamin of claim 1, comprising effective components and auxiliary materials,
the effective components are as follows: immune protein activating factor, compound vitamin, maca powder and pseudo-ginseng powder;
the auxiliary materials are as follows: dextrin and corn starch.
5. The probiotic protein multivitamin of claim 4, wherein the multivitamin comprises one or more of vitamins A, B, C, D, E and K.
6. The probiotic protein multivitamin according to claim 4, which is characterized by comprising the following raw materials in percentage by mass: 80-90% of immune protein activating factor, 2-4% of compound vitamin, 0.5-2% of maca powder, 0.5-2% of pseudo-ginseng powder, 3-8% of dextrin and 2-6% of corn starch.
7. The probiotic protein multivitamin according to claim 4, characterized by comprising the following raw materials in percentage by mass: 85% of immune protein activating factor, 3% of compound vitamin, 1% of maca powder, 1% of pseudo-ginseng powder, 5% of dextrin and 4% of corn starch.
8. The probiotic protein multivitamin of claim 4, in a dosage form including, but not limited to, oral liquid, granules, tablets, buccal formulations or capsules.
9. The probiotic protein multivitamin preparation according to claim 8, wherein the granules of the probiotic protein multivitamin preparation have fineness that all granules pass through a 10-50 mesh sieve, and fine granules which cannot pass through a 10-mesh sieve and can pass through a 65-mesh sieve are not more than 8%.
10. The method of preparing the probiotic protein multivitamin of any one of claims 4 to 9, comprising the steps of:
s1, performing shake flask fermentation on strains, performing fermentation in an S2 fermentation tank, breaking cell walls in S3, performing centrifugal filtration and precipitation in S4, adding multivitamin, maca powder, pseudo-ginseng powder and auxiliary materials in S5, fully stirring and mixing, and S6, performing spray drying and granulation or tabletting.
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