CN115918908A - Quick-soluble sodium hyaluronate, preparation method, application and product - Google Patents
Quick-soluble sodium hyaluronate, preparation method, application and product Download PDFInfo
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- 229920002385 Sodium hyaluronate Polymers 0.000 title claims abstract description 137
- 229940010747 sodium hyaluronate Drugs 0.000 title claims abstract description 137
- YWIVKILSMZOHHF-QJZPQSOGSA-N sodium;(2s,3s,4s,5r,6r)-6-[(2s,3r,4r,5s,6r)-3-acetamido-2-[(2s,3s,4r,5r,6r)-6-[(2r,3r,4r,5s,6r)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2- Chemical compound [Na+].CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 YWIVKILSMZOHHF-QJZPQSOGSA-N 0.000 title claims abstract description 137
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- TUPZEYHYWIEDIH-WAIFQNFQSA-N beta-carotene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1=C(C)CCCC1(C)C)C=CC=C(/C)C=CC2=CCCCC2(C)C TUPZEYHYWIEDIH-WAIFQNFQSA-N 0.000 claims description 8
- 235000013734 beta-carotene Nutrition 0.000 claims description 8
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- OENHQHLEOONYIE-JLTXGRSLSA-N β-Carotene Chemical compound CC=1CCCC(C)(C)C=1\C=C\C(\C)=C\C=C\C(\C)=C\C=C\C=C(/C)\C=C\C=C(/C)\C=C\C1=C(C)CCCC1(C)C OENHQHLEOONYIE-JLTXGRSLSA-N 0.000 claims description 8
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- PZNPLUBHRSSFHT-RRHRGVEJSA-N 1-hexadecanoyl-2-octadecanoyl-sn-glycero-3-phosphocholine Chemical compound CCCCCCCCCCCCCCCCCC(=O)O[C@@H](COP([O-])(=O)OCC[N+](C)(C)C)COC(=O)CCCCCCCCCCCCCCC PZNPLUBHRSSFHT-RRHRGVEJSA-N 0.000 claims 1
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- KIUKXJAPPMFGSW-DNGZLQJQSA-N (2S,3S,4S,5R,6R)-6-[(2S,3R,4R,5S,6R)-3-Acetamido-2-[(2S,3S,4R,5R,6R)-6-[(2R,3R,4R,5S,6R)-3-acetamido-2,5-dihydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-2-carboxy-4,5-dihydroxyoxan-3-yl]oxy-5-hydroxy-6-(hydroxymethyl)oxan-4-yl]oxy-3,4,5-trihydroxyoxane-2-carboxylic acid Chemical compound CC(=O)N[C@H]1[C@H](O)O[C@H](CO)[C@@H](O)[C@@H]1O[C@H]1[C@H](O)[C@@H](O)[C@H](O[C@H]2[C@@H]([C@@H](O[C@H]3[C@@H]([C@@H](O)[C@H](O)[C@H](O3)C(O)=O)O)[C@H](O)[C@@H](CO)O2)NC(C)=O)[C@@H](C(O)=O)O1 KIUKXJAPPMFGSW-DNGZLQJQSA-N 0.000 description 5
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- IAJILQKETJEXLJ-UHFFFAOYSA-N Galacturonsaeure Natural products O=CC(O)C(O)C(O)C(O)C(O)=O IAJILQKETJEXLJ-UHFFFAOYSA-N 0.000 description 2
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- MKYBYDHXWVHEJW-UHFFFAOYSA-N N-[1-oxo-1-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propan-2-yl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(C(C)NC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 MKYBYDHXWVHEJW-UHFFFAOYSA-N 0.000 description 1
- OVRNDRQMDRJTHS-UHFFFAOYSA-N N-acelyl-D-glucosamine Natural products CC(=O)NC1C(O)OC(CO)C(O)C1O OVRNDRQMDRJTHS-UHFFFAOYSA-N 0.000 description 1
- IAJILQKETJEXLJ-QTBDOELSSA-N aldehydo-D-glucuronic acid Chemical compound O=C[C@H](O)[C@@H](O)[C@H](O)[C@H](O)C(O)=O IAJILQKETJEXLJ-QTBDOELSSA-N 0.000 description 1
- AEMOLEFTQBMNLQ-WAXACMCWSA-N alpha-D-glucuronic acid Chemical compound O[C@H]1O[C@H](C(O)=O)[C@@H](O)[C@H](O)[C@H]1O AEMOLEFTQBMNLQ-WAXACMCWSA-N 0.000 description 1
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
- Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change
Landscapes
- Coloring Foods And Improving Nutritive Qualities (AREA)
Abstract
The invention provides a quick-soluble sodium hyaluronate, a preparation method, an application and a product, and relates to the technical field of functional beverages, health products and medicines. Compared with single sodium hyaluronate, the prepared quick-soluble sodium hyaluronate product can be quickly dissolved in water, and the situations of precipitation, agglomeration of particles into blocks and the like do not occur. The invention selects the protein agent as the embedding wall material, improves the solubility of the sodium hyaluronate, increases the nutritive value of the instant sodium hyaluronate, provides high-quality protein for human bodies, and improves the immunity of the human bodies.
Description
Technical Field
The invention relates to the technical field of functional beverages, health-care products and medicines, in particular to a quick-soluble sodium hyaluronate, a preparation method, application and a product.
Background
Hyaluronic acid is commonly called hyaluronic acid, and the molecular formula is (C) 14 H 21 NO 11 ) N is the macromolecular polysaccharide of glucuronic acid consisting of D-glucuronic acid and N-acetyl-D-glucosamine disaccharide. Sodium hyaluronate (C) in the form of its sodium salt is mostly used in industrial production 14 H 2 ONO 11 Na) n for easier storage and processing. By virtue of four unique properties of human self-source property, biocompatibility, strong water locking property and visco-elastic lubricity, the sodium hyaluronate has extremely high application value in a plurality of fields such as medicine, food, daily chemicals and the like. But the terminal market scale in the food field is lower, and the overall maturity also has a great promotion space.
Currently, the related food-grade sodium hyaluronate products are rarely applied by technical means. Sodium hyaluronate has excellent solubility in human body, but the dissolution speed is slow due to excessively strong water retention in the processing process, which is a difficult problem restricting the production of products, and the problem needs to be solved urgently.
At present, the fields of beverages, oral liquids and the like containing hyaluronic acid on the market have no related technical support aiming at the aspect of instant solubility. Based on the method, the technology for embedding the sodium hyaluronate by the microcapsule and the related instant product have important significance for the development of the sodium hyaluronate in the field of food.
In view of the above, the present invention is particularly proposed.
Disclosure of Invention
The invention aims to provide a method for embedding instant sodium hyaluronate, which is used for solving at least one technical problem in the prior art.
The second object of the present invention is to provide a rapidly soluble sodium hyaluronate produced by the above-mentioned embedding method.
The invention also aims to provide the application of the instant sodium hyaluronate in preparing beverages.
The fourth purpose of the invention is to provide a solid beverage.
The fifth purpose of the invention is to provide a liquid beverage.
In order to achieve the above purpose of the present invention, the following technical solutions are adopted:
the invention provides an embedding method of instant sodium hyaluronate, which comprises the following steps: adding a protein agent into the sodium hyaluronate solution, uniformly mixing, carrying out pretreatment, sequentially adding a dissolution accelerator and an emulsifier, uniformly mixing again to obtain a mixed solution, and carrying out spray drying on the mixed solution to obtain the solid powder of the instant sodium hyaluronate.
Further, the molecular weight of the sodium hyaluronate is 11 × 10 4 ～50×10 4 Da；
Preferably, the sodium hyaluronate solution is a sodium hyaluronate aqueous solution, wherein the mass ratio of sodium hyaluronate to water is 1.
Further, the protein agent comprises whey protein, preferably whey protein concentrate;
preferably, the whey protein concentrate has a purity of 75% to 85%, preferably 80%;
preferably, the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1.
Further, the solubilizing agent comprises sucrose;
preferably, the mass ratio of the sodium hyaluronate to the sucrose is 1;
the emulsifier comprises at least one of sucrose ester and soybean lecithin;
preferably, the mass ratio of the sodium hyaluronate to the sucrose ester to the soybean lecithin is 1.
Further, the pretreatment comprises rewarming and pH adjustment after low-temperature treatment;
preferably, the pretreatment comprises returning to room temperature after standing for 6 hours at 4 ℃ and adjusting pH =7.0.
Further, adding an emulsifier and then uniformly mixing in a heating and stirring manner;
preferably, the method further comprises the step of sterilizing the mixed solution before spray drying;
preferably, the mixed solution is subjected to ultrahigh-temperature instantaneous sterilization, cooled to room temperature and then subjected to spray drying;
preferably, the ultrahigh temperature instantaneous sterilization heating temperature is set to be 130-150 ℃, and the heating time is 5-15 seconds;
preferably, the spray drying parameters are set as: the temperature of the air inlet is 170-180 ℃, and the temperature of the air outlet is 80-105 ℃.
The invention also provides the quick-soluble sodium hyaluronate prepared by the embedding method of the quick-soluble sodium hyaluronate.
The invention also provides the application of the instant sodium hyaluronate in preparing beverages;
preferably, the beverage comprises a solid beverage and a liquid beverage;
preferably, the solid beverage comprises instant milk tea and protein powder; the liquid beverage comprises a sports drink.
The invention also provides a solid beverage, which comprises the instant sodium hyaluronate and main ingredients acceptable in food;
preferably, the mass ratio of the instant sodium hyaluronate to the main material is 1.65-5.67;
preferably, the main material comprises milk tea powder or albumen powder.
The invention also provides a liquid beverage, which comprises the instant sodium hyaluronate and auxiliary materials acceptable for food;
preferably, the adjuvants include distilled water, sweeteners and nutritional extracts;
preferably, the sweetener comprises one or more of mogroside, trehalose, and erythritol; the nutritional extract comprises cranberry extract, bighead atractylodes rhizome extract and beta-carotene;
preferably, the addition amount of the instant sodium hyaluronate and mogroside is 0.15-0.5% of the amount of sucrose; and/or, the addition amount of the trehalose is 5-15% of the dosage of the sucrose; and/or the addition amount of the erythritol is 5-12% of the amount of the sucrose; the mass ratio of the mogroside to the trehalose to the erythritol is 0.013;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the cranberry extract is 1;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the extract of the white atractylodes rhizome is 1;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the beta-carotene is 1.
Compared with the prior art, the invention has the following beneficial effects:
1. the invention adopts a spray drying process, embeds the sodium hyaluronate in a microcapsule form, and effectively improves the quick solubility of the sodium hyaluronate. Compared with single sodium hyaluronate, the prepared instant sodium hyaluronate product can be rapidly dissolved in water, and the situations of precipitation, agglomeration of powder particles, block shape and the like can not occur.
2. The invention selects the protein agent as the embedding wall material, improves the solubility of the sodium hyaluronate, increases the nutritive value of the instant sodium hyaluronate, provides high-quality protein for human bodies, and improves the immunity of the human bodies.
3. The invention adds the sodium hyaluronate into milk tea, solid protein beverage and sports beverage, and has the effects of moisturizing, improving skin state, strengthening joints and the like.
Detailed Description
Unless defined otherwise herein, scientific and technical terms used in connection with the present invention shall have the meanings that are commonly understood by one of ordinary skill in the art. The meaning and scope of a term should be clear, however, in the event of any potential ambiguity, the definition provided herein takes precedence over any dictionary or extrinsic definition. In this application, the use of "or" means "and/or" unless stated otherwise. Furthermore, the use of the term "including" and other forms is not limiting.
Generally, the nomenclature used, and the techniques thereof, in connection with the cell and tissue culture, molecular biology, immunology, microbiology, genetics and protein and nucleic acid chemistry and hybridization described herein are those well known and commonly employed in the art. Unless otherwise indicated, the methods and techniques of the present invention are generally performed according to conventional methods well known in the art and as described in various general and more specific references that are cited and discussed throughout the present specification. Enzymatic reactions and purification techniques are performed according to the manufacturer's instructions, as commonly practiced in the art, or as described herein. The nomenclature used in connection with the analytical chemistry, synthetic organic chemistry, and medical and pharmaceutical chemistry described herein, and the laboratory procedures and techniques thereof, are those well known and commonly employed in the art.
The technical solutions of the present invention will be described clearly and completely with reference to the following embodiments, and it should be understood that the described embodiments are some, but not all, embodiments of the present invention. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.
The microcapsule embedding technology utilizes various natural or synthetic high molecular compounds as external phases to completely wrap the target without changing the original chemical properties of the target. The microcapsule embedding technology can improve the stability of products, prevent the mutual interference among all components and change the physical properties (color, shape, density and dispersion performance) of the core material. In the food field, some substances can be integrated into a food system and keep physiological activity. The microcapsule embedding technology is developed for many years, and the spray drying method, the physicochemical method, the interfacial polymerization method and the like are available, wherein the spray drying method is the most mature and is widely applied in the field of foods. The invention realizes the embedding of sodium hyaluronate by microcapsules by a spray drying means. Specifically, according to one aspect of the present invention, there is provided a method for embedding a rapidly soluble sodium hyaluronate, comprising: adding a protein agent into the sodium hyaluronate solution, uniformly mixing, carrying out pretreatment, sequentially adding a dissolution accelerator and an emulsifier, uniformly mixing again to obtain a mixed solution, and carrying out spray drying on the mixed solution to obtain the solid powder of the instant sodium hyaluronate.
The embedding method provided by the invention is simple in process and convenient to operate, and the quick-soluble sodium hyaluronate prepared by the method provided by the invention effectively improves the quick-solubility of sodium hyaluronate, and compared with single sodium hyaluronate, the quick-soluble sodium hyaluronate can be quickly dissolved in water, and the situations of precipitation, agglomeration of particles into blocks and the like are avoided. In addition, the invention selects the protein agent as the embedding wall material, improves the solubility of the sodium hyaluronate, increases the nutritive value of the instant sodium hyaluronate, provides high-quality protein for human bodies, and improves the immunity of the human bodies.
In some preferred embodiments, the molecular weight of the sodium hyaluronate is 11 x 10 4 ～50×10 4 Da, for example, but not limited to, may be 11X 10 4 Da、20×10 4 Da、30×10 4 Da、40×10 4 Da or 50X 10 4 Da。
Preferably, the sodium hyaluronate solution is an aqueous solution of sodium hyaluronate, wherein the mass ratio of sodium hyaluronate to water is 1.
In some preferred embodiments, the protein agent comprises whey protein, which is a fast-dissolving whey protein, which itself has good fast-solubility, and is preferably whey protein concentrate for industrial use;
preferably, the whey protein concentrate has a purity of 75% to 85%, preferably 80%; when the purity of the whey protein concentrate is too low, the encapsulation efficiency is low, raw materials are wasted, and when the concentration of the whey protein concentrate is too high, the whey protein produces gel, so that the powder spraying operation cannot be carried out.
Preferably, the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1.
In some preferred embodiments, the solubilizing agent comprises sucrose; the use of sucrose as a dissolution promoter according to the present invention can increase the solubility of the solution on the one hand and also the taste of the sweet-tasting product on the other hand.
The emulsifier in the invention can reduce electrostatic repulsion between whey protein and polysaccharide, so that the particle size of the whey protein and the polysaccharide is uniform, the emulsifier comprises at least one of sucrose ester and soybean lecithin, and typical emulsifiers can be selected from sucrose ester alone, soybean lecithin alone or sucrose ester and soybean lecithin. When fully selected, the emulsifier used in the present invention further contributes to changing the surface hydrophobicity of the whey protein concentrate with hyaluronic acid.
Preferably, the mass ratio of the sodium hyaluronate to sucrose is 1;
preferably, the mass ratio of the sodium hyaluronate, sucrose ester and soybean lecithin is 1.
In some preferred embodiments, the pretreatment comprises a post-hypothermic rewarming and pH adjustment; the protein and hyaluronic acid can be further fully hydrated and completely dissolved by adopting a treatment mode of rewarming after low-temperature treatment.
Preferably, the pretreatment comprises returning to room temperature after standing for 6 hours at 4 ℃ and adjusting pH =7.0. Wherein, the room temperature is 20-25 ℃.
In some preferred embodiments, the emulsifier is added and then is uniformly mixed by heating and stirring; in the case of heating and stirring, the container is kept sealed to prevent evaporation of water, which leads to increase in the total solid content ratio and generation of gel, and the mouth of the container may be covered with a plastic wrap, for example.
Preferably, the method further comprises the step of sterilizing the mixed solution before spray drying; the mixed solution is preferably sterilized instantaneously at ultra-high temperature, cooled to room temperature and then spray-dried, wherein stirring is continued during heating.
Wherein the ultrahigh temperature instantaneous sterilization heating temperature is set to be 130-150 ℃, and the heating time is 5-15 seconds; the spray drying parameters are set as follows: the temperature of the air inlet is 170-180 ℃, and the temperature of the air outlet is 80-105 ℃.
According to the second aspect of the invention, the quick-soluble sodium hyaluronate prepared by the embedding method of the quick-soluble sodium hyaluronate is also provided.
The instant sodium hyaluronate provided by the invention can be quickly dissolved in water, the situations of precipitation, agglomeration of particles into blocks and the like do not occur, and the instant sodium hyaluronate also has high nutritive value, can provide high-quality protein for human bodies and can improve the immunity of the human bodies.
Based on the beneficial effects of the instant sodium hyaluronate, according to the third aspect of the invention, the invention also provides the application of the instant sodium hyaluronate in preparing a beverage.
The invention adds the sodium hyaluronate into milk tea, solid protein beverage and sports beverage, and has the effects of moisturizing, improving skin state, strengthening joints and the like.
The beverage can be solid beverage or liquid beverage. Specifically, the solid beverage is powder, and is packaged in small bags, such as but not limited to instant milk tea and protein powder; the liquid beverage is a solution, not more than 250ml, bottled, such as but not limited to sports drinks.
It is understood that the instant sodium hyaluronate according to the present invention is preferably used in the field of beverages, and may be used in the fields of food, medicine, etc., based on its instant property.
As a relative specific embodiment, the solid beverage provided by the invention comprises the instant sodium hyaluronate and a food-acceptable main material;
preferably, the mass ratio of the instant sodium hyaluronate to the main material is 1.65-5.67;
preferably, the main material comprises milk tea powder or albumen powder.
As a relative specific embodiment, the liquid beverage provided by the invention comprises the instant sodium hyaluronate and food acceptable auxiliary materials;
preferably, the adjuvants include distilled water, sweeteners and nutritional extracts;
preferably, the sweetener comprises one or more of mogroside, trehalose, and erythritol; the nutritional extract comprises cranberry extract, bighead atractylodes rhizome extract and beta-carotene;
when 50% purity mogroside, 45% sucrose sweet trehalose and 55% sucrose sweet erythritol are selected, the amount of the rapidly soluble sodium hyaluronate and mogroside added is preferably 0.15-0.5% of the amount of sucrose; and/or, the addition amount of the trehalose is 5-15% of the dosage of the sucrose; and/or the addition amount of the erythritol is 5-12% of the amount of the sucrose; the mass ratio of the mogroside to the trehalose to the erythritol is 0.013;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the cranberry extract is 1;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the extract of the white atractylodes rhizome is 1;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the beta-carotene is 1.
It should be noted that, because the daily consumption of sodium hyaluronate by human body is not more than 0.2 g, each bag or bottle of the instant milk tea, protein powder or sports drink provided by the invention contains no more than 0.2 g of sodium hyaluronate. The invention controls the intake of sodium hyaluronate within a reasonable range, does not exceed 0.2 g per day, and exerts the nutrition and health care effects on the premise of ensuring safety.
In addition, the invention also provides a preparation method of the solid beverage or the liquid beverage, and the solid beverage or the liquid beverage is obtained by mixing the instant sodium hyaluronate with main materials or auxiliary materials acceptable for food according to the formula amount.
The invention is further illustrated by the following examples. The materials in the examples are prepared according to known methods or are directly commercially available, unless otherwise specified.
Example 1
The embodiment provides an embedding method of instant sodium hyaluronate, which comprises the following steps:
(1) Dissolving: using a molecular weight of 11X 10 4 Adding Da sodium hyaluronate into distilled water for slow dissolution, then adding 80% whey protein concentrate, and stirring until the whey protein concentrate is completely dissolved, wherein the mass ratio of the sodium hyaluronate to the distilled water is 1;
(2) Hydration: refrigerating the mixed solution obtained in the step (1) for 6 hours at the temperature of 4 ℃;
(3) Adjusting the pH: taking the cold-stored solution in the step (2), returning to room temperature, and adjusting the pH =7.0;
(4) Adding a reagent: adding sucrose, sucrose ester, and soybean lecithin. The mass ratio of the sodium hyaluronate to the sucrose is 1; the mass ratio of the sodium hyaluronate to the sucrose ester to the soybean lecithin is 1;
(5) Ultra-high temperature instant sterilization: the heating temperature is set to be 130-150 ℃, the heating time is 5-15 seconds, the stirring is continuously carried out in the heating process, and then the mixture is cooled to the room temperature;
(6) Spray drying: and (3) removing the mixed solution, spraying powder, and taking the sprayed superfine powder, namely the instant sodium hyaluronate.
Example 2
The embodiment provides an embedding method of instant sodium hyaluronate, which comprises the following steps:
(1) Dissolving: with a molecular weight of 50X 10 4 Da sodium hyaluronate, adding distilled water to slowly dissolve, adding whey protein concentrate with purity of 75%, stirring to completely dissolve, adding sodium hyaluronate andthe mass ratio of the distilled water is 1;
(2) Hydration: refrigerating the mixed solution obtained in the step (1) for 6 hours at the temperature of 4 ℃;
(3) Adjusting the pH: taking the cold-stored solution in the step (2), returning to room temperature, and adjusting the pH =7.0;
(4) Adding a reagent: adding sucrose, sucrose ester, and soybean lecithin. The mass ratio of the sodium hyaluronate to the sucrose is 1; the mass ratio of the sodium hyaluronate to the sucrose ester to the soybean lecithin is 1;
(5) Ultra-high temperature instant sterilization: the heating temperature is set to be 130-150 ℃, the heating time is 5-15 seconds, the stirring is continuously carried out in the heating process, and then the mixture is cooled to the room temperature;
(6) And (3) spray drying: and (3) removing the mixed solution, spraying powder, and taking the sprayed superfine powder, namely the instant sodium hyaluronate.
Example 3
The embodiment provides an embedding method of instant sodium hyaluronate, which comprises the following steps:
(1) Dissolving: with a molecular weight of 35X 10 4 Adding Da sodium hyaluronate into distilled water for slow dissolution, then adding whey protein concentrate with the purity of 85% and stirring until the whey protein concentrate is completely dissolved, wherein the mass ratio of the sodium hyaluronate to the distilled water is 1;
(2) Hydration: refrigerating the mixed solution obtained in the step (1) for 6 hours at 4 ℃;
(3) Adjusting the pH: taking the cold-stored solution in the step (2), returning to room temperature, and adjusting the pH =7.0;
(4) Adding a reagent: adding sucrose, sucrose ester, and soybean lecithin. The mass ratio of the sodium hyaluronate to the sucrose is 1; the mass ratio of the sodium hyaluronate to the sucrose ester to the soybean lecithin is 1;
(5) Ultra-high temperature instant sterilization: the heating temperature is set to be 130-150 ℃, the heating time is 5-15 seconds, the stirring is continuously carried out in the heating process, and then the mixture is cooled to the room temperature;
(6) Spray drying: the obtained mixed solution is taken out for spraying powder, and the sprayed ultrafine powder is taken out, namely the instant sodium hyaluronate.
Comparative example 1
This comparative example provides a method for embedding a rapidly soluble sodium hyaluronate with a molecular weight of 55X 10, which differs from example 1 4 Da。
Comparative example 2
The comparative example provides an embedding method of the instant sodium hyaluronate, which is different from the method of example 1 in that the mass ratio of the sodium hyaluronate to the distilled water is 1.
Comparative example 3
The comparative example provides an embedding method of the instant sodium hyaluronate, which is different from the method of example 1 in that the mass ratio of the sodium hyaluronate to the sucrose is 1.
Comparative example 4
The comparative example provides an embedding method of the instant sodium hyaluronate, which is different from the method of example 1 in that the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1.
Comparative example 5
This comparative example provides a method for embedding a rapidly soluble sodium hyaluronate, which is different from example 1 in that step (2) is not included.
Examples of the experiments
The dissolution performance of the instant sodium hyaluronate prepared by the embedding method of the instant sodium hyaluronate provided by examples 1-3 and comparative examples 1-5 was tested by water absorption index, water solubility index, zeta potential, stability, dispersibility and solubility,
water absorption index and Water solubility index determination methods: accurately weighing 2.0g of the product, placing the product in a centrifuge tube with known constant weight, adding 25mL of distilled water, and shaking until the product powder is completely dispersed in the water. Maintaining the temperature in 30 deg.C water bath for 30min, and oscillating once every 10 min. Centrifuging at 4 000r/min for 30min, collecting supernatant, pouring into a plate with known constant weight, and evaporating in an oven at 105 deg.C to dry to constant weight. The water solubility index and water absorption index are calculated as follows:
in the formula: WSI is water solubility index; WAI is the water absorption index; w0 is the mass of raw material powder, g; w 1 Mass of centrifuge tube, g; w 2 Is the plate mass, g; w is a group of 3 G is the sum of the masses of the plate and the supernatant; w 4 Is the sum of the mass of the centrifuge tube and the sediment, g.
Zeta potential: the method is characterized in that a laser Doppler electrophoresis light scattering technology is adopted for measurement, the temperature is set to be 25 ℃, a sample solution diluted by 100 times is injected into an electrophoresis cell with voltages applied to two ends, and a Zeta potential is directly integrated and calculated from the electrophoresis mobility determined by an experiment by using a Helmholtz-Smoluchowski equation.
Stability and dispersibility: dissolving 0.625g powder in 5mL deionized water, warm bathing at 40 deg.C for 10min, and then vortexing for 2min to dissolve milk powder completely. And (3) dropwise adding the emulsion to an injection port of a laser diffraction particle size analyzer, wherein the dropwise adding amount ensures that the refractive index of the sample is between 10 and 15 percent, and the average value is obtained by measuring each sample for three times.
The solubility determination method refers to the determination of the solubility of infant food and dairy products in national standard for food safety of national standard GB 5413.29-2010.
The results of the experiment are given in the following table:
| group of | Index of water absorption | Index of water solubility | Zeta potential | Stability of | Dispersibility in water | Solubility in water |
| Example 1 | 188.05 | 72.76 | -16.84 | 0.03081 | 0.8313 | 99.8407 |
| Example 2 | 182.62 | 71.52 | -17.192 | 0.04668 | 0.6844 | 99.8789 |
| Example 3 | 187.37 | 73.21 | -21.56 | 0.03484 | 0.5885 | 99.5179 |
| Comparative example 1 | 191.15 | 73.25 | -10.25 | 0.02458 | 0.5485 | 95.244 |
| Comparative example 2 | 145.32 | 56.22 | -8.65 | 0.02415 | 0.5785 | 96.2584 |
| Comparative example 3 | 153.24 | 45.85 | -7.54 | 0.01532 | 0.6215 | 97.2545 |
| Comparative example 4 | 180.52 | 57.22 | -8.25 | 0.01475 | 0.6127 | 95.2257 |
| Comparative example 5 | 135.55 | 45.21 | -5.63 | 0.01658 | 0.5483 | 93.2458 |
As can be seen from the above table, the molecular weight of sodium hyaluronate, the ratio of sodium hyaluronate to distilled water, sucrose, whey protein concentrate and the hydration step all affect the production of instant sodium hyaluronate; from the viewpoint of cost saving, the instant sodium hyaluronate prepared in the embodiment 1 with better effect is applied to the subsequent embodiments.
Example 4 preparation of instant milk tea
Mixing the instant sodium hyaluronate provided by the embodiment 1 of the invention and milk tea powder in a ratio of 1:5.67, and subpackaging 15 g of product powder in a small bag; when in mixing, 200 ml of hot water with the temperature of about 80 ℃ is added into each small bag.
EXAMPLE 5 preparation of protein powder
The instant sodium hyaluronate and protein powder provided by the embodiment 1 of the invention are mixed in a ratio of 1:5.67 weight ratio to obtain product powder, and subpackaging 15 g of the product powder into small bags; when in mixing, about 200 ml of hot water at 80 ℃ is added into each small bag.
Example 6 preparation of sports drink
The instant sodium hyaluronate, mogroside (hunan sinceri biotechnology limited), trehalose (japanese rice-flour-pudding), erythritol (Shandong bowling bio-ken corporation), cranberry extract (shaanxi yang grass biotechnology), white atractylodes rhizome extract (Lanzhou Woltley), and beta-carotene (Lanzhou Woltley) provided in example 1 of the present invention were mixed and dissolved in distilled water to prepare a sport drink. The adding amount of the mogroside is 0.15-0.5% of the using amount of the sucrose, the adding amount of the trehalose is 5-15% of the using amount of the sucrose, and the adding amount of the erythritol is 5-12% of the using amount of the sucrose, wherein the mass ratio of the mogroside to the trehalose to the erythritol is ensured to be 0.013; the mass ratio of the quick-soluble sodium hyaluronate to the cranberry extract is 1: 2-8, wherein the mass ratio of the instant sodium hyaluronate to the white atractylodes rhizome extract is 1: 0.05-0.3, the mass ratio of the instant sodium hyaluronate to the beta-carotene is 1:0.1 to 0.5, and the total volume of each part is not more than 250ml.
Finally, it should be noted that: the above embodiments are only used to illustrate the technical solution of the present invention, and not to limit the same; while the invention has been described in detail and with reference to the foregoing embodiments, it will be understood by those skilled in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some or all of the technical features may be equivalently replaced; and these modifications or substitutions do not depart from the spirit of the corresponding technical solutions of the embodiments of the present invention.
Claims (10)
1. An embedding method of instant sodium hyaluronate is characterized by comprising the following steps: adding a protein agent into the sodium hyaluronate solution, uniformly mixing, then carrying out pretreatment, then adding a dissolution accelerator and an emulsifier, uniformly mixing again to obtain a mixed solution, and carrying out spray drying on the mixed solution to obtain the solid powder of the instant sodium hyaluronate.
2. Embedding method according to claim 1, characterized in that the molecular weight of the sodium hyaluronate is 11 x 10 4 ～50×10 4 Da；
Preferably, the sodium hyaluronate solution is a sodium hyaluronate aqueous solution, wherein the mass ratio of sodium hyaluronate to water is 1.
3. Embedding method according to claim 1, characterized in that the protein agent comprises whey protein, preferably whey protein concentrate;
preferably, the whey protein concentrate has a purity of 75% to 85%, preferably 80%;
preferably, the mass ratio of the sodium hyaluronate to the whey protein concentrate is 1.
4. The embedding method according to claim 1, wherein the dissolution promoting agent comprises sucrose;
preferably, the mass ratio of the sodium hyaluronate to the sucrose is 1;
preferably, the emulsifier comprises at least one of sucrose ester and soy lecithin;
preferably, the mass ratio of the sodium hyaluronate, sucrose ester and soybean lecithin is 1.
5. The embedding method according to claim 1, wherein the pretreatment comprises a temperature recovery and pH adjustment after the low-temperature treatment;
preferably, the pretreatment comprises returning to room temperature after standing for 6 hours at 4 ℃ and adjusting pH =7.0.
6. The embedding method according to claim 1, wherein the emulsifier is added and then the mixture is uniformly mixed by heating and stirring;
preferably, the method further comprises the step of sterilizing the mixed solution before spray drying;
preferably, the mixed solution is subjected to ultrahigh-temperature instantaneous sterilization, cooled to room temperature and then spray-dried;
preferably, the ultrahigh temperature instantaneous sterilization heating temperature is set to be 130-150 ℃, and the heating time is 5-15 seconds;
preferably, the spray drying parameters are set as: the temperature of the air inlet is 170-180 ℃, and the temperature of the air outlet is 80-105 ℃.
7. The instant sodium hyaluronate prepared by the embedding method of the instant sodium hyaluronate according to any one of claims 1 to 6.
8. Use of the instant sodium hyaluronate of claim 7 for the preparation of a beverage;
preferably, the beverage comprises a solid beverage and a liquid beverage;
preferably, the solid beverage comprises instant milk tea and protein powder; the liquid beverage comprises a sports drink.
9. A solid beverage, which is characterized by comprising the instant sodium hyaluronate of claim 7 and a main ingredient acceptable for food;
preferably, the mass ratio of the instant sodium hyaluronate to the main material is 1;
preferably, the main material comprises milk tea powder or albumen powder.
10. A liquid beverage comprising the instant sodium hyaluronate of claim 7 and a food acceptable excipient;
preferably, the adjuvants include distilled water, sweeteners and nutritional extracts;
preferably, the sweetener comprises one or more of mogroside, trehalose, and erythritol; the nutritional extract comprises cranberry extract, bighead atractylodes rhizome extract and beta-carotene;
preferably, the addition amount of the mogroside is 0.15-0.5% of the using amount of the sucrose; and/or, the addition amount of the trehalose is 5-15% of the dosage of the sucrose; and/or the addition amount of the erythritol is 5-12% of the amount of the sucrose; the mass ratio of the mogroside to the trehalose to the erythritol is 0.013;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the cranberry extract is 1;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the extract of the white atractylodes rhizome is 1;
preferably, the mass ratio of the quick-soluble sodium hyaluronate to the beta-carotene is 1.
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