CN115873716A - Penicillium ZC1 strain and application thereof in degradation of green manure residues - Google Patents
Penicillium ZC1 strain and application thereof in degradation of green manure residues Download PDFInfo
- Publication number
- CN115873716A CN115873716A CN202210937244.7A CN202210937244A CN115873716A CN 115873716 A CN115873716 A CN 115873716A CN 202210937244 A CN202210937244 A CN 202210937244A CN 115873716 A CN115873716 A CN 115873716A
- Authority
- CN
- China
- Prior art keywords
- green manure
- penicillium
- strain
- degradation
- green
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 210000003608 fece Anatomy 0.000 title claims abstract description 72
- 239000010871 livestock manure Substances 0.000 title claims abstract description 72
- 230000015556 catabolic process Effects 0.000 title claims abstract description 25
- 238000006731 degradation reaction Methods 0.000 title claims abstract description 25
- 241000228143 Penicillium Species 0.000 title claims abstract description 23
- 229920005610 lignin Polymers 0.000 claims abstract description 11
- 239000001913 cellulose Substances 0.000 claims abstract description 10
- 229920002678 cellulose Polymers 0.000 claims abstract description 10
- 229920002488 Hemicellulose Polymers 0.000 claims abstract description 7
- 238000004321 preservation Methods 0.000 claims description 11
- 239000003795 chemical substances by application Substances 0.000 claims description 4
- 230000000813 microbial effect Effects 0.000 claims description 4
- 244000005700 microbiome Species 0.000 claims description 4
- 241000228168 Penicillium sp. Species 0.000 claims description 3
- 239000004480 active ingredient Substances 0.000 claims description 2
- 239000001963 growth medium Substances 0.000 abstract description 23
- 230000012010 growth Effects 0.000 abstract description 8
- 238000012258 culturing Methods 0.000 abstract description 6
- 238000012360 testing method Methods 0.000 abstract description 5
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 17
- 230000000694 effects Effects 0.000 description 17
- 239000008103 glucose Substances 0.000 description 17
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 16
- 108090000790 Enzymes Proteins 0.000 description 14
- 102000004190 Enzymes Human genes 0.000 description 14
- 229940088598 enzyme Drugs 0.000 description 14
- 239000002689 soil Substances 0.000 description 13
- 239000000843 powder Substances 0.000 description 9
- 238000011282 treatment Methods 0.000 description 9
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 8
- LHGVFZTZFXWLCP-UHFFFAOYSA-N guaiacol Chemical compound COC1=CC=CC=C1O LHGVFZTZFXWLCP-UHFFFAOYSA-N 0.000 description 8
- 239000000243 solution Substances 0.000 description 8
- 238000000855 fermentation Methods 0.000 description 7
- 230000004151 fermentation Effects 0.000 description 7
- 238000009630 liquid culture Methods 0.000 description 7
- 230000001580 bacterial effect Effects 0.000 description 6
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 description 6
- 238000000354 decomposition reaction Methods 0.000 description 6
- 239000012153 distilled water Substances 0.000 description 6
- 239000002609 medium Substances 0.000 description 6
- 229920001817 Agar Polymers 0.000 description 5
- 240000001238 Gaultheria procumbens Species 0.000 description 5
- 235000007297 Gaultheria procumbens Nutrition 0.000 description 5
- 239000000284 extract Substances 0.000 description 5
- 239000000463 material Substances 0.000 description 5
- 238000000034 method Methods 0.000 description 5
- OSWPMRLSEDHDFF-UHFFFAOYSA-N methyl salicylate Chemical compound COC(=O)C1=CC=CC=C1O OSWPMRLSEDHDFF-UHFFFAOYSA-N 0.000 description 5
- 239000000203 mixture Substances 0.000 description 5
- 239000007787 solid Substances 0.000 description 5
- 239000006228 supernatant Substances 0.000 description 5
- 241000233866 Fungi Species 0.000 description 4
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 4
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 4
- 244000061456 Solanum tuberosum Species 0.000 description 4
- 235000002595 Solanum tuberosum Nutrition 0.000 description 4
- 239000008272 agar Substances 0.000 description 4
- 239000002585 base Substances 0.000 description 4
- 229960001867 guaiacol Drugs 0.000 description 4
- 239000007788 liquid Substances 0.000 description 4
- 229910052757 nitrogen Inorganic materials 0.000 description 4
- 230000001954 sterilising effect Effects 0.000 description 4
- UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
- 239000000758 substrate Substances 0.000 description 4
- 230000007306 turnover Effects 0.000 description 4
- 108010059892 Cellulase Proteins 0.000 description 3
- 241000196324 Embryophyta Species 0.000 description 3
- PEDCQBHIVMGVHV-UHFFFAOYSA-N Glycerine Chemical compound OCC(O)CO PEDCQBHIVMGVHV-UHFFFAOYSA-N 0.000 description 3
- 108010029541 Laccase Proteins 0.000 description 3
- 108010054320 Lignin peroxidase Proteins 0.000 description 3
- 241000209082 Lolium Species 0.000 description 3
- 108010059896 Manganese peroxidase Proteins 0.000 description 3
- CKUAXEQHGKSLHN-UHFFFAOYSA-N [C].[N] Chemical compound [C].[N] CKUAXEQHGKSLHN-UHFFFAOYSA-N 0.000 description 3
- 229940041514 candida albicans extract Drugs 0.000 description 3
- 229940106157 cellulase Drugs 0.000 description 3
- 239000003153 chemical reaction reagent Substances 0.000 description 3
- 238000001816 cooling Methods 0.000 description 3
- 230000000593 degrading effect Effects 0.000 description 3
- 238000001035 drying Methods 0.000 description 3
- 239000003337 fertilizer Substances 0.000 description 3
- 108010062085 ligninase Proteins 0.000 description 3
- 230000000877 morphologic effect Effects 0.000 description 3
- 238000003825 pressing Methods 0.000 description 3
- 108090000623 proteins and genes Proteins 0.000 description 3
- XOSXWYQMOYSSKB-LDKJGXKFSA-L water blue Chemical compound CC1=CC(/C(\C(C=C2)=CC=C2NC(C=C2)=CC=C2S([O-])(=O)=O)=C(\C=C2)/C=C/C\2=N\C(C=C2)=CC=C2S([O-])(=O)=O)=CC(S(O)(=O)=O)=C1N.[Na+].[Na+] XOSXWYQMOYSSKB-LDKJGXKFSA-L 0.000 description 3
- 239000012138 yeast extract Substances 0.000 description 3
- 241000894006 Bacteria Species 0.000 description 2
- 239000002028 Biomass Substances 0.000 description 2
- 241000219193 Brassicaceae Species 0.000 description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 description 2
- 239000001888 Peptone Substances 0.000 description 2
- 108010080698 Peptones Proteins 0.000 description 2
- 241000209504 Poaceae Species 0.000 description 2
- 241000219000 Populus Species 0.000 description 2
- 244000088415 Raphanus sativus Species 0.000 description 2
- 235000006140 Raphanus sativus var sativus Nutrition 0.000 description 2
- 241000219793 Trifolium Species 0.000 description 2
- 229930003451 Vitamin B1 Natural products 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 2
- BFNBIHQBYMNNAN-UHFFFAOYSA-N ammonium sulfate Chemical compound N.N.OS(O)(=O)=O BFNBIHQBYMNNAN-UHFFFAOYSA-N 0.000 description 2
- 229910052921 ammonium sulfate Inorganic materials 0.000 description 2
- 235000011130 ammonium sulphate Nutrition 0.000 description 2
- 239000000872 buffer Substances 0.000 description 2
- 229910052799 carbon Inorganic materials 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 238000010586 diagram Methods 0.000 description 2
- 210000003527 eukaryotic cell Anatomy 0.000 description 2
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 2
- 235000019341 magnesium sulphate Nutrition 0.000 description 2
- 238000004519 manufacturing process Methods 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 2
- 235000019796 monopotassium phosphate Nutrition 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 235000019319 peptone Nutrition 0.000 description 2
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 description 2
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 238000012216 screening Methods 0.000 description 2
- 238000007873 sieving Methods 0.000 description 2
- 239000011780 sodium chloride Substances 0.000 description 2
- 239000010902 straw Substances 0.000 description 2
- 229960005322 streptomycin Drugs 0.000 description 2
- 229960003495 thiamine Drugs 0.000 description 2
- DPJRMOMPQZCRJU-UHFFFAOYSA-M thiamine hydrochloride Chemical compound Cl.[Cl-].CC1=C(CCO)SC=[N+]1CC1=CN=C(C)N=C1N DPJRMOMPQZCRJU-UHFFFAOYSA-M 0.000 description 2
- 239000011691 vitamin B1 Substances 0.000 description 2
- 235000010374 vitamin B1 Nutrition 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- ZTOJFFHGPLIVKC-YAFCTCPESA-N (2e)-3-ethyl-2-[(z)-(3-ethyl-6-sulfo-1,3-benzothiazol-2-ylidene)hydrazinylidene]-1,3-benzothiazole-6-sulfonic acid Chemical compound S\1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C/1=N/N=C1/SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-YAFCTCPESA-N 0.000 description 1
- 229920000936 Agarose Polymers 0.000 description 1
- 241001061264 Astragalus Species 0.000 description 1
- 235000010110 Astragalus glycyphyllos Nutrition 0.000 description 1
- 235000008744 Brassica perviridis Nutrition 0.000 description 1
- 241000712024 Brassica rapa var. perviridis Species 0.000 description 1
- 235000002566 Capsicum Nutrition 0.000 description 1
- LZZYPRNAOMGNLH-UHFFFAOYSA-M Cetrimonium bromide Chemical compound [Br-].CCCCCCCCCCCCCCCC[N+](C)(C)C LZZYPRNAOMGNLH-UHFFFAOYSA-M 0.000 description 1
- 108020004414 DNA Proteins 0.000 description 1
- 108091023242 Internal transcribed spacer Proteins 0.000 description 1
- 238000012408 PCR amplification Methods 0.000 description 1
- 239000006002 Pepper Substances 0.000 description 1
- 102000003992 Peroxidases Human genes 0.000 description 1
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 description 1
- 235000016761 Piper aduncum Nutrition 0.000 description 1
- 235000017804 Piper guineense Nutrition 0.000 description 1
- 244000203593 Piper nigrum Species 0.000 description 1
- 235000008184 Piper nigrum Nutrition 0.000 description 1
- 240000004713 Pisum sativum Species 0.000 description 1
- 235000016815 Pisum sativum var arvense Nutrition 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 244000007853 Sarothamnus scoparius Species 0.000 description 1
- 241000168254 Siro Species 0.000 description 1
- 241001460073 Trichoderma asperellum Species 0.000 description 1
- 241000219873 Vicia Species 0.000 description 1
- 240000002895 Vicia hirsuta Species 0.000 description 1
- 241001464837 Viridiplantae Species 0.000 description 1
- ZTOJFFHGPLIVKC-CLFAGFIQSA-N abts Chemical compound S/1C2=CC(S(O)(=O)=O)=CC=C2N(CC)C\1=N\N=C1/SC2=CC(S(O)(=O)=O)=CC=C2N1CC ZTOJFFHGPLIVKC-CLFAGFIQSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000003213 activating effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 238000000137 annealing Methods 0.000 description 1
- 235000006533 astragalus Nutrition 0.000 description 1
- 238000003287 bathing Methods 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 description 1
- 238000009835 boiling Methods 0.000 description 1
- 239000012295 chemical reaction liquid Substances 0.000 description 1
- 238000007621 cluster analysis Methods 0.000 description 1
- 230000019771 cognition Effects 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 238000004925 denaturation Methods 0.000 description 1
- 230000036425 denaturation Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- BNIILDVGGAEEIG-UHFFFAOYSA-L disodium hydrogen phosphate Chemical compound [Na+].[Na+].OP([O-])([O-])=O BNIILDVGGAEEIG-UHFFFAOYSA-L 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000001962 electrophoresis Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 230000035558 fertility Effects 0.000 description 1
- 238000011049 filling Methods 0.000 description 1
- 239000000706 filtrate Substances 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 230000002538 fungal effect Effects 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 238000012966 insertion method Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 235000021374 legumes Nutrition 0.000 description 1
- 239000004570 mortar (masonry) Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 238000010899 nucleation Methods 0.000 description 1
- 239000003895 organic fertilizer Substances 0.000 description 1
- 239000011368 organic material Substances 0.000 description 1
- 239000005416 organic matter Substances 0.000 description 1
- 108040007629 peroxidase activity proteins Proteins 0.000 description 1
- 229910052698 phosphorus Inorganic materials 0.000 description 1
- 239000011574 phosphorus Substances 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 238000003752 polymerase chain reaction Methods 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000012257 pre-denaturation Methods 0.000 description 1
- 239000000047 product Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 238000000746 purification Methods 0.000 description 1
- 230000035484 reaction time Effects 0.000 description 1
- 238000004626 scanning electron microscopy Methods 0.000 description 1
- 238000007789 sealing Methods 0.000 description 1
- 238000011218 seed culture Methods 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000011734 sodium Substances 0.000 description 1
- 238000009331 sowing Methods 0.000 description 1
- 239000012086 standard solution Substances 0.000 description 1
- 238000003860 storage Methods 0.000 description 1
- 239000013589 supplement Substances 0.000 description 1
- 239000004577 thatch Substances 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- DGVVWUTYPXICAM-UHFFFAOYSA-N β‐Mercaptoethanol Chemical compound OCCS DGVVWUTYPXICAM-UHFFFAOYSA-N 0.000 description 1
Images
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses a penicillium ZC1 and application thereof in degradation of green manure residues, wherein the strain has high growth speed, and filter paper can be completely disintegrated by culturing the strain in a filter paper strip degradation culture medium at 28 ℃ for 5 days; in a green manure residue degradation test, the lignin degradation rate of ZC1 on green manure residues reaches 78.12%, and the cellulose and hemicellulose degradation rates reach 85.17% and 84.46% respectively.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and relates to a Trichoderma asperellum ZC1 strain and application thereof in degradation of green manure residues.
Background
Green manure is a green plant that uses all or overground green bodies produced during the growth of plants to directly or indirectly turn over and press the green bodies into soil to be used as fertilizer or to perform interplanting rotation with main crops, and has the effects of promoting the growth of the main crops, improving the soil properties and the like (Cao Weidong and the like, 2010). The green manure crops are a valuable organic matter resource in the agricultural ecosystem and are rich in carbon, nitrogen, phosphorus, potassium and other trace elements required by the growth of main crops. The green manure is used as a clean organic fertilizer source, plays an important role in the aspects of soil fertility improvement and fertilizer replacement, and is one of important characteristics of modern agriculture.
China has rich green manure resources, 916 kinds of common green manure crops are counted, and 70 kinds of green manure crops (Cao Weidong and the like, 2017) which can adapt to climatic environments of different regions and have good growth conditions are obtained after growth adaptability screening. The green manure suitable for planting at present has various varieties, mainly including leguminous and gramineous. The leguminous green manure has the advantages of nitrogen fixation, large biomass, low carbon-nitrogen ratio and capability of inputting a large amount of carbon and nitrogen into soil. Common legume green manure cultivated abroad is mainly clover and field pea (Rodrigues et al 2015, hogue et al 2010, wells, 2012. The common green manure in China mainly comprises smooth-leaf vetch, hairy vetch, milk vetch and perennial clover. The gramineous green manure has the advantages of high carbon-nitrogen ratio, developed root system, mainly fibrous roots, not deep distribution in a soil layer and no adverse effect on the root system of main crops. Common gramineous green manure is mainly ryegrass and thatch (Tworkoski et al, 2012, ripoche et al, 2011, gouthu et al, 2012;).
At present, the green manure is mainly utilized in three ways of green pressing, covering and self-seeding (Zhou Zhixiang, etc., 1997; poplar leaf bloom, 2020). The green pressing is divided into on-site turning pressing and mowing and burying. The on-site turnover refers to directly turning over the fresh green manure into the soil through turnover machinery or manual labor, wherein the turnover depth is about 20 cm; the mowing and burying refers to that all or part of the upper part of the green manure is mown and then is buried in the soil. The covering is divided into tree disc covering and natural withering covering, wherein the tree disc covering refers to manually mowing green manure and then covering the tree disc of a main crop (poplar leaf bloom, 2020); natural withering and covering refers to that plant residues are kept on the soil after the plant residues naturally die after the growth period of the plant residues is finished by green manure.
The average yield of the common Chinese green manure is 38.0t/hm 2 Wherein the grass family is highest, the average yield of the green manure of the ryegrass of the grass family reaches 53.2t/hm 2 Secondly, the yield of the green manure of the leguminous is 46.8 to 48.2t/hm 2 Meanwhile, the yield of the green manure of Brassicaceae rape, feita radish and February is lower than 20.0t/hm because the Brassicaceae rape, feita radish and February are the lowest 2 The difference is not obvious and is only about 1/3 of the yield of the ryegrass (Yangyua et al, 2020). Compared with other methods, the method for utilizing the green manure with large biomass by naturally decomposing the green manure in the field after being covered by the withered natural cover can not only fertilize soil, supplement soil organic matters and provide nutrients for main crops, but also save cost, and is a main method for simplifying planting and utilization of the green manure.
Compared with other organic materials such as straw, the green manure has the characteristics of low carbon-nitrogen ratio and quick microbial decomposition. However, due to the different degrees of old and tender of the green manure, young and tender green stems and leaves are easy to decay after naturally withering and returning to the field, but the withered and old stems and leaves have more cellulose and lignin and less water and are difficult to decay. In addition, drought, soil over-acid and over-alkali, and over-high or low temperature all affect the decomposition of green manure. Because the winter green manure is withered and aged She Jiaoduo and the temperature is lower in winter, the field natural decomposition speed is lower. Generally, the winter green manure is sown in 9-10 months every year, and naturally withers and covers in 6-7 months in the next year, and the slow decomposition of the winter green manure can directly influence the sowing of the summer green manure and the colonization and binding of seeds, so that the winter green manure needs to be decomposed quickly and converted into soil organic matters, the nutrient is supplemented for the soil, and the planting of the summer green manure is not influenced.
Because of the limitation of practical conditions such as agricultural technology, insufficient cognition of farmers and the like, the green manure industry in China develops slowly, and related green manure residue degrading bacteria are rarely reported at present. Therefore, a high-efficiency green manure residue degrading bacterium needs to be screened out so as to assist the development of the green manure industry.
Disclosure of Invention
In view of the above, the present invention aims to provide a penicillium ZC1 strain and its use in the degradation of green manure residues.
In order to achieve the purpose, the invention provides the following technical scheme:
1. the Penicillium ZC1 strain is classified and named as Penicillium (Penicillium sp.) ZC1, is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, and has the preservation number of CGMCC No.40237, the preservation date of 2022 years, 7 months and 1 day, and the preservation address of the institute of microbiology, china academy of sciences No.3, north West Lu No.1 Hospital, chaozhou, beijing.
Preferably, the ITS sequence of the penicillium ZC1 strain is shown in SEQ ID NO. 1.
ggggacctgcggaaggatcattactgagtgagggcccctcGGGGtccaacCTCCCACCCGTGTTTAACGAACCTTTGTTGCTTCGGCGGGCCCGCCTCACGGCCGCCGGGGGGCTCCTGCCCCCGGGCCCGCGCCCGCCGAAGCCCCCCCTTGAACGCTGTCTGAAGTTTGCAGTCTGAGAAACTAGCTAAATTAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCCGTCCCCCCCTCTGCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTCGTCACCCGCTCTTGTAGGCCCGGCCGGCGCCAGCCGACCCCAACCCTAAatttttttcaggttgacctcggatcaggtagggatacccgctgaacttaagcatatcataaaacc, shown in SEQ ID NO. 1.
2. The application of the penicillium ZC1 strain in degradation of lignin, cellulose or hemicellulose.
3. Application of a penicillium ZC1 strain in degradation of green manure residues.
4. A microbial agent comprises the active ingredient of the penicillium ZC1 strain, and the mass content of the microbial agent is 5-10%.
The invention has the beneficial effects that:
the invention obtains a new strain of Penicillium, namely Penicillium sp ZC1, by screening and separating green manure residues naturally decomposed in fields in Chongqing. The strain has high growth speed, and conidia can be fully paved on a culture dish with the diameter of 8cm within 72 hours. The bacterial colony is gray green, simple long and upright conidiophores are generated on aerial hyphae, the top ends of the conidiophores are branched in a special symmetrical or asymmetrical broom-shaped mode and are called as broom branches, the branches are multipolar conidiophores, and finally a plurality of phialides are generated, and conidiophores are grown on the phialides, are spherical to oval and are green. The colony was brown on the back.
Culturing in filter paper strip degradation culture medium at 28 deg.C for 5 days to completely disintegrate the filter paper; in a green manure residue degradation test, the lignin degradation rate of ZC1 on green manure residues reaches 78.12%, and the cellulose and hemicellulose degradation rates reach 85.17% and 84.46% respectively.
Drawings
In order to make the object, technical solution and advantages of the present invention more clear, the present invention provides the following drawings for illustration.
FIG. 1 shows the plate color reaction of ZC1 strain guaiacol and aniline blue;
FIG. 2 is a diagram of the cellulose and ligninase activities of a ZC1 strain, wherein A is the cellulose activity and B is the ligninase activity;
FIG. 3 is a phylogenetic tree of the ZC1 strain;
FIG. 4 is a structure diagram of an electron microscope of pepper twigs before and after the ZC1 strain treatment, wherein A is CK non-inoculated treatment and B is ZC1 strain treatment.
FIG. 5 is a glucose standard curve.
Biological preservation information
And (3) classification and naming: penicillium (Penicillium sp.) ZC1;
the preservation number is as follows: CGMCC No.40237;
preservation time: 7 months and 1 day 2022;
the preservation unit: china general microbiological culture Collection center;
and (4) storage address: the institute of microbiology, national academy of sciences, no.3, west Lu No.1, beijing, chaoyang, beicheng, area, beichen.
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
Isolation and identification of ZC1 Strain
(1) Test material
Collecting natural decomposed green manure residues in the fields of Chongqing city, selecting completely decomposed materials which have high decomposition degree, black brown color, bacterial plaque on the surface, moist and soft texture and no pungent smell, putting the completely decomposed materials into a sealed bag, and storing the completely decomposed materials in a refrigerator at 4 ℃ in a laboratory.
(2) Culture medium
Enrichment culture medium: 200g of potato extract, 20g of glucose, 0.33g of streptomycin and 1000mL of distilled water, and the pH is natural.
Lignin selection medium: 2g of alkaline lignin, 1.33g of ammonium sulfate, 0.5g of magnesium sulfate, 1g of monopotassium phosphate, 0.2g of disodium hydrogen phosphate, 0.33g of streptomycin and 20g of agar, and adding distilled water to reach the volume of 1000mL (refer to Guo Xiaowei and the like, 2017, wang Jing and the like, 2020).
Gu-PDA medium: 200g of potato extract and 20g of agar, 6g of dried green manure powder (the green manure is collected from Hechuan base of laboratory farm of southwest university, collected withered stems and leaves of winter green manure, cleaned, dried to constant weight at 65 ℃, crushed for later use by a sieve of 40 mm) is used for replacing glucose, guaiacol is 0.4m, water is added to the mixture to be constant volume of 1L, and the mixture is sterilized for 3min at 121 ℃ for qualitative determination of laccase/peroxidase production capacity of the strain (as shown in the upper half part of a figure 1).
AB-PDA medium: 200g of potato extract and 20g of agar, 6g of dried green fertilizer powder is used for replacing glucose, 0.1g of aniline blue is added, and the volume is adjusted to 1L by adding water.
PDA culture medium: 200g of potato extract, 20g of glucose and 20g of agar (agar is not added in a liquid culture medium, and 8g/L of agar is added in a semi-solid culture medium).
(3) Separation and purification of ZC1 bacterial strain
Weighing 10g of decomposed green manure residue sample, culturing in 90mL of enrichment medium at 28 ℃ and 120r/min for 48h, and selecting a dilution gradient of 10 -1 、10 -3 、10 -5 、10 -7 And 10 -9 Mu.l of the culture solution is smeared on a lignin selection medium respectively and cultured for 48h at 28 ℃. Selecting strains with obvious fungus morphology on Gu-PDA and AB-PDA culture medium, selecting strains with obvious color development circle and color change circle, repeatedly purifying on PDA culture medium until pure culture to obtain glycerol bacterial liquid, and storing at-80 deg.C. Specifically, as shown in fig. 1, the upper half shows guaiacol color and the lower half shows aniline blue color.
The selected strain is named ZC1 and preserved in China general microbiological culture Collection center (CGMCC for short, address: no.3 Siro 1 of Beijing city Kogyo-sunward, kyoho) within 1 month 7 in 2022 years, the preservation number is CGMCC No.40237, and the selected strain is classified and named as Penicillium sp ZC1.
Example 2
Identification of ZC1 Strain
1) Morphological identification
Activating the preserved strain ZC1 by a PDA culture medium, and observing the morphological characteristics of the colony; then, the culture is carried out by the plate insertion method, and the morphological structure of the culture is observed under a microscope.
ZC1 strain is shown in figure 1, wherein colonies are grayish green, simple long and upright conidiophores are generated on aerial hyphae, the top ends of the conidiophores are branched in a special symmetrical or asymmetrical broom-shaped mode and are called broom-shaped branches, the conidiophores branched into multiple poles finally generate a plurality of phialides, conidial chains are grown on the phialides, and the conidia are spherical to oval and are green. The colony was brown on the back. The growth speed is high, and the conidia can be paved on a culture dish with the diameter of 8cm within 72 hours.
2) Physiological and biochemical identification
a. And (3) cellulase activity determination:
(1) seed culture medium: 5g of peptone, 10g of beef extract, 5g of yeast powder, 5g of glucose, 5g of sodium chloride and 1000mL of distilled water, and the pH value is 7.0.
Fermentation enzyme-producing culture medium: 3g of peptone, 0.5g of yeast extract powder, 2.0g of ammonium sulfate, 4g of monopotassium phosphate, 0.3g of sodium chloride, 0.3g of magnesium sulfate, 20g of CMC-Na and 1000mL of distilled water.
(2) 5 pieces of 1 cm-diameter fungi were inoculated into a seed medium and cultured at 36 ℃ at 160r/min for 18 hours to prepare a seed solution. Adding 3 percent of inoculum size (volume) of seed liquid into 200mL of fermentation enzyme production culture medium, culturing for 24h at 36 ℃ and 160r/min, centrifuging for 10min at 4 ℃ and 5000r/min, and taking supernatant to measure enzyme activity.
(3) The filter paper enzyme activity determination method comprises the following steps: 5mL of fermentation broth extracted from the sample was added to each centrifuge tube. And (5) putting the mixture into a centrifuge and operating the centrifuge for 10min at the speed of 5000r/min to obtain supernatant, namely the crude enzyme solution required by the test. 0.5mL of crude enzyme solution was extracted to measure cellulase activity, and 50mg of Xinhua filter paper was used as a substrate and placed in 4 tubes with 20mL stoppers, while 0.5mL of enzyme solution and 1.5mL of citric acid buffer (0.05 mol/L of citric acid buffer, formulation method Liu Di, 2008) were added to the 4 tubes. One of the tubes was filled with 1.5mL DNS reagent as a control. Preheating 4 test tubes in a water bath at 50 ℃, adding 50mg of filter paper after 10min, reacting for 60min at the temperature, taking out samples, immediately adding 2.0mL of DNS reagent into each sample, placing in a water bath at 100 ℃ again for 5min, cooling after taking out, metering the volume to 15mL, and measuring the absorbance of each sample by a spectrophotometer. The measured OD values were compared with a glucose standard curve, and the OD values were converted into glucose concentrations. The amount of enzyme required to produce 1. Mu. MoL of glucose per hour of substrate is expressed as one unit of enzyme activity (U) FPA as follows:
in the formula: g is the glucose content; v: fixing the volume; t: reaction time; a: adding enzyme; b: mass of substrate; 5.56 is the amount of 1mg of glucose base material (. Mu. Mol).
(4) And (3) preparing a glucose standard curve: 0.1mg/mL of glucose standard solution (0 mL, 0.2 mL, 0.4 mL, 0.6 mL, 0.8 mL, 1.0 mL, 1.2 mL and 1.5 mL) was pipetted into a colorimetric tube, 1.5mL was replenished with distilled water, 2mL of DNS reagent was added, mixed well and boiled in boiling water for 5min. The volume is reduced to 15mL by cooling. OD was measured with a spectrophotometer at 540 nm. And drawing a glucose standard curve by taking the content (mg) of glucose as a horizontal coordinate and the OD value as a vertical coordinate, and fitting a regression equation. As shown in fig. 5.
The cellulase activity results are shown in FIG. 2A.
b. And (3) ligninase activity determination:
(1) liquid culture medium: 20g of glucose, 5g of yeast extract powder 2 PO 4 1g，MgSO 4 ·7H 2 O 0.5g，ZnSO 4 ·7H 2 O50 mg, adding water to a constant volume of 1L, naturally adding PH, subpackaging into 250mL conical flasks, pouring 100mL of liquid culture medium into each flask, sterilizing at 121 ℃ for 30min, and adding 40 mu L of vitamin B1.
Solid culture medium of green manure residues: drying the green manure residues at 60 ℃ to constant weight, crushing, sieving by a 40-mesh sieve, adding the green manure residues to a culture dish according to the ratio (mass ratio) of green manure residues to water =1:2.5, and sterilizing at 121 ℃ for 30min.
(2) Inoculating 5 fungus blocks with diameter of 1cm into liquid culture medium, and performing shake culture at 25 deg.C and 150r/min for 6d.
(3) And (2) according to the volume of seed fermentation liquor: inoculating the seed fermentation liquor into a green manure residue solid culture medium according to the mass ratio (mass ratio) of 1:1, culturing at 25 ℃ for 30d, taking out 3 parts of pretreated green manure residue samples every 5d, weighing 1g of the samples in a centrifuge tube, adding 3mL of distilled water, shaking at 25 ℃ and 150r/min for 4h, filtering 4 layers of yarns, centrifuging the filtrate at 4 ℃ for 10min at 3000r/min, taking the supernatant, and storing the supernatant, namely the crude extracellular enzyme solution in a refrigerator at 4 ℃ (Cong Shan 2014, she Jianjiang and the like 2018). The activity of laccase, manganese peroxidase (MnP) and lignin peroxidase (LiP) in the pretreated green manure residue sample is measured by using a kit (Chongqing Amida Biotechnology Co., ltd.). Definition of laccase activity units (U): mu. Mol of 2,2 '-diaza-bis-3-ethylbenzothiazoline-6-sulfonic acid (2,2' -azino-bis (3-ethylbenzothiazoline-6-sulfonic acid), ABTS) substrate was oxidized at a wavelength of 420nm per minute. Definition of MnP activity units (U): the amount of enzyme required to oxidize 1. Mu. Mol of guaiacol per minute at a wavelength of 465 nm. Definition of the unit of LiP activity (U): at the wavelength of 651nm, the change of absorbance value per minute in each 1mL reaction system is 0.01 which is an enzyme activity unit.
The results of enzyme activity are shown in B in FIG. 2.
And (3) data analysis: the software SPSS 22.0 was used to calculate the mean and standard deviation and analyze the significance of the differences between the treatments.
3) Molecular biological identification of fungal ITS
Inoculating the purified strain on 100mL PDA liquid culture medium,the culture was carried out overnight. The funnel was filtered and the residual culture liquid was blotted dry, transferred to a mortar and then liquid nitrogen was added rapidly to grind the tissue into powder, and the ground powder was immediately transferred to a 1.5ml Eppendorf tube. Adding 2 times volume of preheated 2% CTAB (containing 0.1% by mass of beta-mercaptoethanol), mixing thoroughly, bathing at 65 ℃ for 30-45min, mixing uniformly every 15min, cooling to room temperature, centrifuging at 10000rmp for 10min, transferring the supernatant to another clean 1.5mL Eppendorf tube, adding an equal volume of Tris-chloroform-isoamyl alcohol mixture (volume ratio 25 2 The O solution dissolves DNA.
The ITS sequence is a small gene segment positioned between 18S,5.8S and 28S of the IDNA coding gene, and the ITS sequence in eukaryotic cells is conserved and is not influenced by the change of environmental conditions. Therefore, the distance of relationship and the phylogenetic position of ITS base sequence in eukaryotic cells can be analyzed by analyzing the homology of ITS base sequence with other microbial species through universal primers.
The ITS universal primers are as follows:
ITS1:5'GGAAGTAAAAGTCGTAACAAGG-3' as shown in SEQ ID NO. 2;
ITS4:5'TCCTCC GCTTATTGATGC-3' as shown in SEQ ID NO. 3.
The PCR amplification procedure was: after 5min of pre-denaturation at 94 ℃: beginning to cycle 94 ℃ denaturation 30s,52 ℃ annealing 60s,72 ℃ extension for 2min, totally 30 cycles, 72 ℃ extension for 10min, taking 2 microliter of reaction liquid to carry out agarose electrophoresis detection with the mass percentage of 1%, sending PCR amplification products to Shanghai Meiji bioengineering Co., ltd for sequencing, inputting the sequence into GenBank for sequence homology comparison and analysis.
4) Construction of ITS sequence phylogenetic Tree
And respectively downloading ITS sequences of strains with sequence similarity of more than 97 percent with the sequence of the degrading strain ZC1 from a Genbank gene database. After cluster analysis by ClustalX, phylogenetic evolutionary trees were generated using MEGA4.1 software. The results are shown in FIG. 3.
Example 3
Determination of green manure residue powder degradation capability of ZC1 strain
Liquid culture medium: 20g of glucose, 5g of yeast extract powder 2 PO 4 1g，MgSO 4 ·7H 2 O 0.5g，ZnSO 4 ·7H 2 O50 mg, adding water to a constant volume of 1L, adding PH, dispensing into 250mL conical bottles, pouring liquid culture medium into each bottle of 100mL, sterilizing at 121 ℃ for 30min, and adding vitamin B1 of 40 mu L.
Solid culture medium of green manure residues: drying the green manure residues at 60 ℃ to constant weight, crushing, sieving by a 40-mesh sieve, adding the green manure residues to a culture dish according to the ratio (mass ratio) of water =1:2.5, and sterilizing at 121 ℃ for 30min.
And (2) according to the volume of seed fermentation liquor: inoculating the seed fermentation liquor into a green manure residue solid culture medium according to the weight of the green manure residue =1:1, culturing at 28 ℃ for 40d, sampling every 10d (sampling at the 5 th time), taking out the sample after 40d, drying at 60 ℃ to constant weight, and filling into a self-sealing bag for later use. The degradation of the sample was observed by scanning electron microscopy, as shown in FIG. 4, wherein A is CK without bacterial treatment, and FIG. 2 is ZC1 strain after bacterial treatment.
And determining the lignin, cellulose and hemicellulose contents of the pretreated green manure residues, as shown in tables 1-3: ( Note: CK1 is 2 generation white rot fungus NDM3-2, purchased from Beijing Biotech center; CK2 is a composite straw decomposition agent, purchased from Zhong Xi Biotechnology Co., ltd, the same as below )
TABLE 1 ZC1 treatment of Green manure residue for 40 days Lignin degradation
TABLE 2 ZC1 treatment of Green manure residue for 40 days cellulose degradation
TABLE 3 degradation rate of hemicellulose by ZC1 treatment of green manure residues for 40 days
As can be seen from tables 1-3, the Penicillium ZC1 strain of the invention has obvious advantages in the aspects of lignin degradation, cellulose degradation and hemicellulose degradation of green manure residues.
Finally, it is noted that the above-mentioned preferred embodiments illustrate rather than limit the invention, and that, although the invention has been described in detail with reference to the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and detail may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (5)
1. The Penicillium ZC1 strain is characterized in that the classification is named as Penicillium (Penicillium sp.) ZC1, the Penicillium ZC1 strain is preserved in the common microorganism center of China Committee for culture Collection of microorganisms, the preservation number is CGMCC No.40237, the preservation date is 2022 years, 7 months and 1 day, and the preservation address is the institute of microbiology of the institute of China academy of sciences No.3, north Cheng West Lu No.1 Hospital, chaoyang, beijing.
2. The penicillium ZC1 strain according to claim 1, wherein the ITS sequence of the penicillium ZC1 strain is as shown in SEQ ID No. 1.
ggggacctgcggaaggatcattactgagtgagggcccctcGGGGtccaacCTCCCACCCGTGTTTAACGAACCTTTGTTGCTTCGGCGGGCCCGCCTCACGGCCGCCGGGGGGCTCCTGCCCCCGGGCCCGCGCCCGCCGAAGCCCCCCCTTGAACGCTGTCTGAAGTTTGCAGTCTGAGAAACTAGCTAAATTAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCCGTCCCCCCCTCTGCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTCGTCACCCGCTCTTGTAGGCCCGGCCGGCGCCAGCCGACCCCAACCCTAAatttttttcaggttgacctcggatcaggtagggatacccgctgaacttaagcatatcataaaacc, shown in SEQ ID NO. 1.
3. Use of the penicillium ZC1 strain according to claim 1 or 2 for the degradation of lignin, cellulose or hemicellulose.
4. Use of the penicillium ZC1 strain according to claim 1 or 2 for the degradation of green manure residues.
5. A microbial agent characterized by comprising the Penicillium ZC1 strain of claim 1 or 2 as an active ingredient in an amount of 5 to 10% by mass.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210937244.7A CN115873716B (en) | 2022-08-05 | 2022-08-05 | Penicillium ZC1 strain and application thereof in degradation of green manure residues |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202210937244.7A CN115873716B (en) | 2022-08-05 | 2022-08-05 | Penicillium ZC1 strain and application thereof in degradation of green manure residues |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115873716A true CN115873716A (en) | 2023-03-31 |
| CN115873716B CN115873716B (en) | 2024-03-01 |
Family
ID=85769590
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202210937244.7A Active CN115873716B (en) | 2022-08-05 | 2022-08-05 | Penicillium ZC1 strain and application thereof in degradation of green manure residues |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115873716B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005130737A (en) * | 2003-10-29 | 2005-05-26 | Hodogaya Chem Co Ltd | Penicilium bilaii for promoting degradation of lawn grass thatch and formulation containing the same |
| BRPI0805560A2 (en) * | 2008-12-29 | 2010-09-08 | Petroleo Brasileiro Sa | production process of an enzymatic preparation for cellulose hydrolysis of lignocellulosic waste and its application in ethanol production |
| CN102586123A (en) * | 2012-02-24 | 2012-07-18 | 熊鹏 | Secondary screening method of strain with high yield of lignocellulose degrading enzyme |
| CN102703344A (en) * | 2012-05-10 | 2012-10-03 | 上海交通大学 | Straw degradation actinomycete and application thereof |
| EP2658984A1 (en) * | 2010-12-31 | 2013-11-06 | Clariant Produkte (Deutschland) GmbH | Efficient lignocellulose hydrolysis with integrated enzyme production |
| US20150203833A1 (en) * | 2010-07-30 | 2015-07-23 | Clean-Vantage Llc | Aspergillus containing beta-glucosidase, beta-glucosidases and nucleic acids encoding the same |
| CN107937279A (en) * | 2017-11-27 | 2018-04-20 | 农业部规划设计研究院 | A kind of maize straw degraded acidifying microbial inoculum and preparation method thereof |
| CN108812029A (en) * | 2018-07-10 | 2018-11-16 | 西南大学 | A kind of reservoir area of Three Gorges sloping upland citrus orchard Green manuring method |
| CN114381378A (en) * | 2021-12-27 | 2022-04-22 | 农业农村部环境保护科研监测所 | Penicillium chrysogenum capable of degrading lignin and application thereof |
-
2022
- 2022-08-05 CN CN202210937244.7A patent/CN115873716B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP2005130737A (en) * | 2003-10-29 | 2005-05-26 | Hodogaya Chem Co Ltd | Penicilium bilaii for promoting degradation of lawn grass thatch and formulation containing the same |
| BRPI0805560A2 (en) * | 2008-12-29 | 2010-09-08 | Petroleo Brasileiro Sa | production process of an enzymatic preparation for cellulose hydrolysis of lignocellulosic waste and its application in ethanol production |
| US20150203833A1 (en) * | 2010-07-30 | 2015-07-23 | Clean-Vantage Llc | Aspergillus containing beta-glucosidase, beta-glucosidases and nucleic acids encoding the same |
| EP2658984A1 (en) * | 2010-12-31 | 2013-11-06 | Clariant Produkte (Deutschland) GmbH | Efficient lignocellulose hydrolysis with integrated enzyme production |
| CN102586123A (en) * | 2012-02-24 | 2012-07-18 | 熊鹏 | Secondary screening method of strain with high yield of lignocellulose degrading enzyme |
| CN102703344A (en) * | 2012-05-10 | 2012-10-03 | 上海交通大学 | Straw degradation actinomycete and application thereof |
| CN107937279A (en) * | 2017-11-27 | 2018-04-20 | 农业部规划设计研究院 | A kind of maize straw degraded acidifying microbial inoculum and preparation method thereof |
| CN108812029A (en) * | 2018-07-10 | 2018-11-16 | 西南大学 | A kind of reservoir area of Three Gorges sloping upland citrus orchard Green manuring method |
| CN114381378A (en) * | 2021-12-27 | 2022-04-22 | 农业农村部环境保护科研监测所 | Penicillium chrysogenum capable of degrading lignin and application thereof |
Non-Patent Citations (2)
| Title |
|---|
| KAISA MARJAMAA等: "Novel penicillium cellulases for total hydrolysis of lignocellulosics", ENZYME MICROB TECHNOL, 13 March 2013 (2013-03-13) * |
| 樊亚娟: "青霉菌秸秆发酵物中酶协同氧化降解木质素机制研究", 中国优秀硕士学位论文全文数据库 工程科技I辑, 15 February 2019 (2019-02-15) * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115873716B (en) | 2024-03-01 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN105567612B (en) | A kind of degradation composite bacteria agent preparation of garden waste and application | |
| CN112410244B (en) | Bacillus aryabhattai and application thereof | |
| CN111876351A (en) | Bacillus belgii and application thereof in relieving apple continuous cropping obstacle | |
| CN105647832B (en) | One plant of high temperature resistant garden waste decomposer FHM1 and its application | |
| CN109796289A (en) | It is a kind of using oil-tea camellia husks as bio-fertilizer of raw material and preparation method thereof | |
| CN102174423B (en) | Bacillus licheniformis CH15 for degrading straws and bacterial agent thereof | |
| CN105567609B (en) | One plant of high temperature resistant garden waste decomposer ST2 and its application | |
| CN102174403A (en) | Application of waste compost composite bacterial agent to improving drought resistance of lawn grass | |
| CN111363694B (en) | Stagnant corynebacterium, screening method and application thereof | |
| CN106967637B (en) | Pear branch degrading bacterium L2 and microbial inoculum thereof | |
| CN105670966B (en) | High-temperature-resistant garden waste decomposing bacteria ST4 and application thereof | |
| CN103173387B (en) | Growth-promoting bacteria for facilitating growth of rape and microbial organic fertilizer | |
| KR100252469B1 (en) | Microbial preparation for manufacturing compost and its manufacturing method | |
| CN107586748B (en) | A kind of China's sporangium and its application | |
| CN115772473A (en) | Trichoderma asperellum ZC3 strain and application thereof in pepper branch degradation | |
| CN105567608B (en) | High-temperature-resistant garden waste decomposing bacteria ST1 and application thereof | |
| CN115873716B (en) | Penicillium ZC1 strain and application thereof in degradation of green manure residues | |
| CN112481173B (en) | Bacillus endophyticus GBW-F008 for producing indoleacetic acid and application thereof | |
| CN111808758B (en) | Crop growth-promoting endophytic fungus and application thereof | |
| CN112715758B (en) | Lactobacillus buchneri BL4 and method for preparing silage by using same | |
| CN113880614A (en) | Method for preparing organic fertilizer by utilizing penicillin fungi residue compost | |
| CN105567610B (en) | One plant of high temperature resistant garden waste decomposer ST5 and its application | |
| CN105567607B (en) | High-temperature-resistant garden waste decomposing bacteria ST3 and application thereof | |
| CN108841750A (en) | A kind of effective biological agent and preparation method thereof for decomposing rice straw | |
| CN108707560A (en) | A kind of microbial liquid bacterial manure and its preparation method and application |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |