CN115873716B - Penicillium ZC1 strain and application thereof in degradation of green manure residues - Google Patents
Penicillium ZC1 strain and application thereof in degradation of green manure residues Download PDFInfo
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Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02W—CLIMATE CHANGE MITIGATION TECHNOLOGIES RELATED TO WASTEWATER TREATMENT OR WASTE MANAGEMENT
- Y02W30/00—Technologies for solid waste management
- Y02W30/40—Bio-organic fraction processing; Production of fertilisers from the organic fraction of waste or refuse
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- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses penicillium ZC1 and application thereof in degradation of green manure residues, wherein the strain has high growth speed, and can completely disintegrate filter paper after being cultured for 5 days at 28 ℃ in a filter paper degradation culture medium; in the green manure residue degradation test, the lignin degradation rate of ZC1 on the green manure residue reaches 78.12%, and the cellulose and hemicellulose degradation rates respectively reach 85.17% and 84.46%.
Description
Technical Field
The invention belongs to the technical field of microorganisms, and relates to trichoderma asperellum ZC1 strain and application thereof in degradation of green manure residues.
Background
Green manure is a green plant (Cao Weidong, 2010) which is produced in the plant growth process and is directly or indirectly turned and pressed into soil to be used as fertilizer or is rotated with a main crop by interplanting the green plant and the main crop to promote the growth of the main crop, improve the soil properties and the like. The green manure crop is a valuable organic resource in the agricultural ecological system, and is rich in carbon, nitrogen, phosphorus, potassium and other microelements required by the growth of main crops. The green manure is used as a clean organic fertilizer source, plays an important role in fertility improvement and replacing chemical fertilizers, and is one of important characteristics of modern agriculture.
The green manure resources in China are rich, 916 types of green manure crops are counted, and after growth adaptability screening, more than 70 green manure crops (Cao Weidong and the like, 2017) which can adapt to the climatic environments of different areas and have good growth conditions are obtained. The green manure suitable for planting at present has various varieties, mainly comprising leguminous plants and gramineous plants. The leguminous green manure has the advantages of nitrogen fixation, large biomass, low carbon nitrogen ratio and capability of inputting a large amount of carbon and nitrogen into soil. The most common cultivated leguminous green manure in foreign countries is mainly clover and field peas (Rodrigues et al 2015; hogue et al 2010; wells 2012). The domestic common green manure mainly comprises vetch, mao She seed, astragalus sinicus and perennial clover. The Gramineae green manure has the advantages of higher self carbon nitrogen ratio, developed root system, taking fibrous roots as the main materials, deep distribution in soil layers and no adverse effect on the root system of main crops. Common green manure for growing grasses is mainly composed of ryegrass and thatch (Tworkoski et al 2012;Ripoche et al, 2011;Gouthu et al, 2012).
At present, green manure is mainly used in three modes of green pressing, covering and self-propagation (Zhou Zhixiang, etc., 1997; yang Shehua, 2020). The green pressing is divided into two types of in-situ turning pressing and mowing landfill. In-situ turning refers to turning fresh green manure into soil directly by a turning machine or manual work, wherein the turning depth is about 20 cm; cutting and landfill refers to the process of intensively burying all or part of the upper parts of the green manure into soil after cutting. The covering is divided into a tree plate covering and a natural wilting covering, wherein the tree plate covering refers to manually cutting green manure and then covering the green manure at the tree plate (Yang Shehua, 2020) of the main crops; natural wilt coverage refers to the retention of plant residues on the soil following the natural death of the green manure itself in its growth phase.
The average yield of the Chinese common green manure is 38.0t/hm 2 Wherein the average yield of the green fertilizer of the ryegrass of the Gramineae is 53.2t/hm 2 Secondly, the yield of the leguminous green manure is 46.8 to 48.2t/hm 2 The yield of the rape, the field-fattened radish and the green manure of the february in the cruciferae is lower than 20.0t/hm 2 And the difference is not obvious, which is only about 1/3 of the ryegrass yield (Yang Shehua, etc., 2020). For green manure with large biomass, the method is similar to other modesCompared with the method, the method for utilizing natural decay in the field after natural wilting and covering can not only fertilize soil, supplement soil organic matters and provide nutrients for main crops, but also save cost, and is a main method for planting and utilizing green manure in a light and simplified manner.
Compared with other organic materials such as straw, the green manure has the characteristics of low carbon-nitrogen ratio and quick microbial decomposition. However, because of different degrees of tenderness of the green manure, the tender green stems and leaves after naturally withering and returning to the field are easy to decompose, but the cellulose and lignin of the old stems and leaves are more, the moisture is less, and the decomposition is difficult. In addition, drought, soil peracid, excessive alkali, excessive temperature or low temperature can affect the decomposition of green manure. As the winter green manure has more old stems and leaves and lower temperature in winter, the natural decomposition speed in the field is slower. The winter green manure is sown in 9-10 months each year, natural withering is carried out in 6-7 months of the second year, and the slow decomposition of the winter green manure can directly influence the sowing of the summer green manure and the colonization and downward bundling of seeds, so that the winter green manure is required to be quickly decomposed and converted into soil organic matters, and the planting of the summer green manure is not influenced while the nutrients are supplemented for the soil.
Because of the limitation of practical conditions such as agricultural technology, insufficient cognition of farmers and the like, the green manure industry in China is slow to develop, and the related green manure residue degrading bacterial agent is freshly reported. Based on the above, it is needed to screen out a green manure residue high-efficiency degradation bacterium so as to assist the development of green manure industry.
Disclosure of Invention
In view of the above, the present invention aims to provide a penicillium ZC1 strain and its application in degradation of green manure residues.
In order to achieve the above purpose, the present invention provides the following technical solutions:
1. penicillium ZC1 strain, which is classified and named as Penicillium sp.) ZC1, is preserved in China general microbiological culture Collection center (CGMCC) with a preservation number of CGMCC No.40237 and a preservation date of 2022, 7 months and 1 day, and has a preservation address of China academy of microorganisms, national institute No.3, north Chen West Lu 1, the Korean region of Beijing.
Preferably, the ITS sequence of the penicillium ZC1 strain is shown as SEQ ID NO. 1.
ggggacctgcggaaggatcattactgagtgagggcccctcGGGGtccaacCTCCCACCCGTGTTTAACGAACCTTTGTTGCTTCGGCGGGCCCGCCTCACGGCCGCCGGGGGGCTCCTGCCCCCGGGCCCGCGCCCGCCGAAGCCCCCCCTTGAACGCTGTCTGAAGTTTGCAGTCTGAGAAACTAGCTAAATTAGTTAAAACTTTCAACAACGGATCTCTTGGTTCCGGCATCGATGAAGAACGCAGCGAAATGCGATAACTAATGTGAATTGCAGAATTCAGTGAATCATCGAGTCTTTGAACGCACATTGCGCCCTCTGGTATTCCGGAGGGCATGCCTGTCCGAGCGTCATTGCTGCCCTCAAGCACGGCTTGTGTGTTGGGCCCCCGTCCCCCCCTCTGCCGGGGGGACGGGCCCGAAAGGCAGCGGCGGCACCGCGTCCGGTCCTCGAGCGTATGGGGCTTCGTCACCCGCTCTTGTAGGCCCGGCCGGCGCCAGCCGACCCCAACCCTAAatttttttcaggttgacctcggatcaggtagggatacccgctgaacttaagcatatcataaaacc, as shown in SEQ ID NO. 1.
2. The application of the strain Penicillium ZC1 in lignin, cellulose or hemicellulose degradation.
3. The application of the penicillium ZC1 strain in degrading green manure residues.
4. The microbial agent comprises the active ingredient of the penicillium ZC1 strain, and the mass content of the penicillium ZC1 strain is 5-10%.
The invention has the beneficial effects that:
the invention screens and separates a new strain of Penicillium from green manure residues naturally decomposed in field in Chongqing city, namely Penicillium sp. The strain has high growth speed, and conidium can be spread on a culture dish with the diameter of 8cm within 72 hours. The bacterial colony is grey green, a simple long upright conidiophore is generated on the aerial hypha, the top end of the bacterial colony is branched in a special symmetrical or asymmetrical broom-shaped mode, the special broom-shaped branch is called as broom-shaped branch, the conidiophore branched into multipolar branches finally generates a plurality of bottle peduncles, a conidiophore chain is adhered on the bottle peduncles, and the conidiophores are spherical to oval and green. The back of the colony was brown.
Culturing in filter paper degradation medium at 28deg.C for 5d to completely disintegrate filter paper; in the green manure residue degradation test, the lignin degradation rate of ZC1 on the green manure residue reaches 78.12%, and the cellulose and hemicellulose degradation rates respectively reach 85.17% and 84.46%.
Drawings
In order to make the objects, technical solutions and advantageous effects of the present invention more clear, the present invention is illustrated in the following drawings.
FIG. 1 shows the color reaction of guaiacol and aniline blue plates of ZC1 strain;
FIG. 2 is a diagram of cellulose and lignin enzyme activities of ZC1 strain, wherein A is cellulase activity and B is lignin enzyme activity;
FIG. 3 is a ZC1 strain phylogenetic tree;
FIG. 4 is a diagram showing the structure of ZC1 strain in which A is the treatment of CK without inoculation and B is the treatment of ZC1 strain.
FIG. 5 is a glucose standard curve.
Biological preservation information
Classification naming: penicillium sp ZC1;
preservation number: CGMCC No.40237;
preservation time: 2022, 7, 1;
preservation unit: china general microbiological culture Collection center (China Committee for culture Collection);
preservation address: the institute of microorganisms of national academy of sciences of China, no.1, no.3, north Chen West Lu, the Korean region of Beijing.
Detailed Description
Preferred embodiments of the present invention will be described in detail below with reference to the accompanying drawings.
Example 1
Isolation and identification of ZC1 Strain
(1) Test material
Collecting green manure residues naturally decomposed in field in Chongqing city, selecting completely decomposed materials with high decomposition degree, black brown color, plaque on surface, moist and soft texture and no pungent smell, placing into a sealed bag, and storing in a refrigerator at 4deg.C in laboratory.
(2) Culture medium
Enrichment medium: 200g of potato extract, 20g of glucose, 0.33g of streptomycin, 1.000 mL of distilled water and natural pH.
Lignin selection medium: 2g of alkaline lignin, 1.33g of ammonium sulfate, 0.5g of magnesium sulfate, 1g of monopotassium phosphate, 0.2g of disodium hydrogen phosphate, 0.33g of streptomycin, 20g of agar and distilled water are added to fix the volume to 1000mL (see Guo Xiaowei and the like, 2017; wang Jing and the like, 2020).
Gu-PDA medium: 200g of potato extract, 20g of agar, 6g of dried green manure powder (the green manure is collected from a southwest university experiment farm Hechuan base, old stems and leaves of winter green manure are collected, cleaned, dried to constant weight at 65 ℃, crushed and screened by a 40mm sieve for later use) is used for replacing glucose, 0.4m of guaiacol is added, water is added to a volume of 1L, sterilization is carried out at 121 ℃ for 3min, and the laccase/peroxidase production capability of the strain is qualitatively measured (as shown in the upper half part of fig. 1).
AB-PDA medium: 200g of potato extract, 20g of agar, 6g of dried green manure powder instead of glucose, 0.1g of aniline blue and water are added to fix the volume to 1L.
PDA medium: 200g of potato extract, 20g of glucose and 20g of agar (agar is not added to the liquid medium, 8g/L of agar is added to the semisolid medium).
(3) Isolation and purification of ZC1 Strain
Weighing 10g of decomposed green manure residue sample, culturing in 90mL enrichment medium at 28deg.C for 48 hr/min, and selecting dilution gradient of 10 -1 、10 -3 、10 -5 、10 -7 And 10 -9 100 μl of each was applied to lignin selection medium and incubated at 28deg.C for 48h. Selecting strain with obvious fungus form on Gu-PDA and AB-PDA culture mediums, selecting strain with obvious color circle and color circle, repeatedly purifying on PDA culture medium until pure culture to obtain glycerol bacterial liquid, and preserving strain at-80deg.C. Specifically, as shown in FIG. 1, guaiacol was developed in the upper half, and aniline blue was developed in the lower half.
The selected strain is named ZC1 and preserved in China general microbiological culture Collection center (CGMCC) at the date of 7 months and 1 day 2022, wherein the preservation number is CGMCC No.40237 and the classification is named as Penicillium sp.
Example 2
Identification of ZC1 Strain
1) Morphological identification
Activating the preserved strain ZC1 by a PDA culture medium, and observing colony morphological characteristics of the strain; then, the cells were cultured by the insert method, and the morphological structure was observed under a microscope.
As shown in FIG. 1, ZC1 strain forms, bacterial colony is gray green, simple long and upright conidiophores are generated on aerial hyphae, the top ends of the bacterial colony are branched in a special symmetrical or asymmetrical broom-shaped mode, the bacterial colony is called broom-shaped branch, the conidiophores branched into multipolar conidiophores finally generate a plurality of bottle peduncles, conidiophores are adhered to the bottle peduncles, and the conidiophores are spherical to oval and green. The back of the colony was brown. The growth speed is high, and the conidium can be spread on a culture dish with the diameter of 8cm within 72 hours.
2) Physiological and biochemical identification
a. Cellulase activity assay:
(1) seed culture medium: 5g of peptone, 10g of beef extract, 5g of yeast powder, 5g of glucose, 5g of sodium chloride and 1000mL of distilled water with the pH of 7.0.
Fermentation enzyme-producing culture medium: 3g of peptone, 0.5g of yeast extract powder, 2.0g of ammonium sulfate, 4g of monopotassium phosphate, 0.3g of sodium chloride, 0.3g of magnesium sulfate, 20g of CMC-Na and 1000mL of distilled water.
(2) 5 pieces of 1 cm-diameter bacterial pieces were inoculated into a seed medium and cultured at 36℃for 18 hours at 160r/min to prepare a seed solution. The enzyme activity was measured by adding 3% of seed solution (volume) to 200mL of fermentation enzyme-producing medium, culturing at 36℃at 160r/min for 24 hours, centrifuging at 4℃at 5000r/min for 10min, and collecting the supernatant.
(3) The filter paper enzyme activity determination method comprises the following steps: 5mL of fermentation broth extracted from the sample was added to each centrifuge tube. And (3) placing the mixture into a centrifugal machine to run for 10min at 5000r/min to obtain supernatant which is the crude enzyme liquid required by the test. In the crude enzyme solution, 0.5mL is extracted to measure the activity of the cellulase, 50mg of Xinhua filter paper is taken as a substrate, the substrate is placed in 420 mL test tubes with plugs, and simultaneously 0.5mL of the enzyme solution and 1.5mL of citric acid buffer solution (0.05 mol/L of citric acid buffer solution, reference Liu and 2008) are respectively added into the 4 test tubes. 1.5mL of DNS reagent was added to one of the tubes as a control. 4 test tubes are preheated in a water bath at 50 ℃ for 10min, 50mg of filter paper is added and reacted at the temperature for 60min, 2.0mL of DNS reagent is added into each sample immediately after the sample is taken out, the reaction is carried out in a water bath at 100 ℃ for 5min again, the sample is taken out and cooled, the volume is fixed to 15mL, and the absorbance of each sample is measured by a spectrophotometer. The measured OD values were compared by a plotted glucose standard curve and converted to glucose concentration. The amount of enzyme required to produce 1. Mu. MoL of glucose per hour of substrate is expressed as one enzyme activity unit (U) FPA as follows:
wherein: g is the glucose content; v: a constant volume; t: reaction time; a: adding enzyme amount; b: substrate mass; 5.56 is the amount (. Mu. Mol) of 1mg of glucose bottom substance.
(4) Establishing a glucose standard curve: 0.1mg/mL glucose standard solution 0, 0.2, 0.4, 0.6, 0.8, 1.0, 1.2 and 1.5mL are respectively sucked, placed in a colorimetric tube, supplemented to 1.5mL by distilled water, 2mL of DNS reagent is added, and boiled in boiling water for 5min after full mixing. Cooled to a constant volume of 15mL. OD was measured at 540nm using a spectrophotometer. Glucose content (mg) is plotted on the abscissa, OD value is plotted on the ordinate, and a regression equation is fitted. As shown in fig. 5.
The cellulase activity results are shown in FIG. 2A.
b. Lignin enzyme activity assay:
(1) liquid medium: 20g of glucose, 5g of yeast extract powder and KH 2 PO 4 1g，MgSO 4 ·7H 2 O 0.5g，ZnSO 4 ·7H 2 O50 mg, adding water to a constant volume of 1L, naturally packaging into 250mL conical flasks, pouring 100mL of liquid culture medium into each flask, sterilizing at 121 ℃ for 30min, and adding 1 mu L of vitamin B.
Green manure residue solid medium: drying the green manure residue at 60 ℃ to constant weight, crushing, sieving with a 40-mesh sieve, adding the green manure residue into a culture dish according to the ratio of water=1:2.5 (mass ratio), and sterilizing at 121 ℃ for 30min.
(2) 5 bacterial blocks with the diameter of 1cm are taken and inoculated into a liquid culture medium for 6d of shaking culture at 25 ℃ and 150 r/min.
(3) The volume of the seed fermentation liquid is as follows: inoculating seed fermentation liquid into a green manure residue solid culture medium according to the ratio of the green manure residue mass=1:1 (mass ratio), culturing for 30 days at 25 ℃, taking out 3 parts of pretreated green manure residue samples every 5 days, weighing 1g of the samples into a centrifuge tube, adding distilled water for 3mL, shaking for 4 hours at 25 ℃ and 150r/min, filtering by 4 layers of yarns, centrifuging the filtrate for 10 minutes at 3000r/min at 4 ℃, taking supernatant, and preserving the obtained liquid as extracellular crude enzyme liquid in a refrigerator at 4 ℃ (Cong Shan 2014; she Jianjiang and other 2018). Laccase, manganese peroxidase (manganese peroxidase, mnP) and lignin peroxidase (lignin peroxidase, liP) activities in the pretreated green manure residue samples were determined using a kit (Chongqing Almida Biotechnology Co., ltd.). Definition of laccase viability unit (U): at a wavelength of 420nm, 1. Mu. Mol of 2,2 '-biazino-bis-3-ethylbenzothiazoline-6-sulfonic acid (2, 2' -azino-bis (3-ethylazothiazoline-6-sulfonic acid), ABTS) substrate was oxidized per minute. Definition of MnP activity units (U): the amount of enzyme required for oxidizing 1. Mu. Mol of guaiacol per minute at a wavelength of 465 nm. Definition of LiP viability units (U): the absorbance value change per 1mL of the reaction system per minute at the wavelength of 651nm is 0.01 as an enzyme activity unit.
The results of the enzyme activities are shown in FIG. 2B.
Data analysis: the mean and standard deviation were calculated using software SPSS 22.0 and the significance of the differences between the individual treatments was analyzed.
3) Molecular biology identification of fungal ITS
The purified strain was inoculated onto 100mL PDA liquid medium and cultured overnight. The remaining culture broth was filtered off in a funnel and sucked dry, and after transfer to a mortar, the tissue was ground to a powder by rapid addition of liquid nitrogen, and immediately the ground powder was transferred to a 1.5ml Eppendorf tube. Adding 2 times volume of preheated 2% CTAB (containing 0.1% beta-mercaptoethanol) by mass concentration, mixing thoroughly, warm-bathing at 65deg.C for 30-45min, mixing once every 15min, cooling to room temperature, centrifuging at 10000rmp for 10min, transferring supernatant to another clean Eppendorf tube of 1.5mL, adding an equal volume of Tris phenol-chloroform-isoamyl alcohol mixed solution (volume ratio of 25:24:1), extracting at 10000rmp, centrifuging for 10min, transferring supernatant to another clean Ep of 1.5mLIn a pendorf tube. The equal volume of chloroform-isoamyl alcohol mixture (volume ratio 24:1) was extracted once more, mixed upside down for 10min, centrifuged at 10000rmp for 10min, and the supernatant was transferred to another Eppendorf tube of 1.5mL. Adding 0.6-0.7 times of pre-cooled isopropanol to precipitate DNA, mixing, and standing at-20deg.C overnight. Centrifuge 10000rnp for 10min, discard supernatant. The precipitate was desalted by washing with 75% ethanol aqueous solution by volume, dehydrated by washing with absolute ethanol, and dried in a 37℃incubator for 10 minutes. Add 50. Mu.L ddH 2 The O solution dissolves the DNA.
ITS sequences are small gene fragments located between IDNA encoding genes 18S,5.8S and 28S, and are conserved in eukaryotic cells and are not affected by changes in the environmental conditions. Therefore, the homology of ITS base sequences in eukaryotic cells and ITS sequences among other microorganism species can be analyzed by adopting universal primers, and the distances and phylogenetic status of the genetic relationship of the ITS base sequences and the ITS sequences can be analyzed.
The ITS universal primer is as follows:
ITS1:5'GGAAGTAAAAGTCGTAACAAGG-3' shown in SEQ ID NO. 2;
ITS4:5'TCCTCC GCTTATTGATATGC-3' as shown in SEQ ID NO. 3.
The PCR amplification procedure was: after 5min of pre-denaturation at 94 ℃): beginning to circularly denature at 94 ℃ for 30s, annealing at 52 ℃ for 60s, extending at 72 ℃ for 2min for 30 cycles, extending at 72 ℃ for 10min, taking 2 mu L of reaction liquid to carry out mass percent 1% agarose electrophoresis detection, sending PCR amplified products to Shanghai Meiji biological engineering Co., ltd for sequencing, and inputting the sequences into GenBank for sequence homology comparison analysis.
4) Construction of ITS sequence phylogenetic tree
ITS sequences of strains with sequence similarity of more than 97% with the ZC1 strain of degradation are downloaded from Genbank gene database respectively. After cluster analysis by ClustalX, phylogenetic tree was generated using MEGA4.1 software. The results are shown in FIG. 3.
Example 3
Determination of green manure residue powder degradation capability of ZC1 strain
Liquid medium: 20g of glucose, 5g of yeast extract powder and KH 2 PO 4 1g，MgSO 4 ·7H 2 O 0.5g，ZnSO 4 ·7H 2 O50 mg, adding water to a constant volume of 1L, naturally packaging into 250mL conical flasks, pouring 100mL of liquid culture medium into each flask, sterilizing at 121 ℃ for 30min, and adding 1 mu L of vitamin B.
Green manure residue solid medium: drying the green manure residue at 60 ℃ to constant weight, crushing, sieving with a 40-mesh sieve, adding the green manure residue into a culture dish according to the ratio of water=1:2.5 (mass ratio), and sterilizing at 121 ℃ for 30min.
The volume of the seed fermentation liquid is as follows: inoculating the seed fermentation liquid into a solid culture medium of the green manure residue at the ratio of the green manure residue mass=1:1, culturing for 40d at 28 ℃, sampling every 10d (adding the 5 th d sample), taking out the sample after 40d, drying at 60 ℃ to constant weight, and filling into a self-sealing bag for standby. The degradation condition of the sample is observed by a scanning electron microscope, as shown in fig. 4, wherein A is CK which is not inoculated, and fig. 2 is ZC1 strain after treatment.
And the content of lignin, cellulose and hemicellulose of the pretreated green manure residue is measured as shown in tables 1-3: ( And (3) injection: CK1 is 2-generation white rot fungus NDM3-2, and is purchased from Beijing biological collection; CK2 is a composite straw-decomposing inoculant available from Zhong Xi Biotechnology Co., ltd )
TABLE 1 lignin degradation rate of green manure residue treated with ZC1 for 40 days
TABLE 2 cellulose degradation rate of green manure residue treated with ZC1 for 40 days
TABLE 3 hemicellulose degradation rate of green manure residue treated with ZC1 for 40 days
From tables 1-3, the penicillium ZC1 strain of the invention has obvious advantages in the aspects of lignin degradation, cellulose degradation and hemicellulose degradation of green manure residues.
Finally, it is noted that the above-mentioned preferred embodiments are only intended to illustrate rather than limit the invention, and that, although the invention has been described in detail by means of the above-mentioned preferred embodiments, it will be understood by those skilled in the art that various changes in form and details may be made therein without departing from the scope of the invention as defined by the appended claims.
Claims (4)
1. Penicillium plantPenicillium sp.) The strain is characterized in that the strain is classified and named as penicillium ZC1, and is preserved in China general microbiological culture Collection center (CGMCC) No.40237, the preservation date is 2022, 7 and 1, and the preservation address is the national institute of microbiology, national academy of sciences 3, national institute of sciences, 1 st Caragana, beijing, chaoyang.
2. Use of the penicillium strain according to claim 1 for lignin, cellulose or hemicellulose degradation.
3. Use of a penicillium strain according to claim 1 for degradation of green manure residues.
4. A microbial agent is characterized in that the effective component of the microbial agent is the penicillium strain of claim 1, and the mass content of the microbial agent is 5-10%.
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