CN102174403A - Application of waste compost composite bacterial agent to improving drought resistance of lawn grass - Google Patents
Application of waste compost composite bacterial agent to improving drought resistance of lawn grass Download PDFInfo
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- CN102174403A CN102174403A CN2011100370289A CN201110037028A CN102174403A CN 102174403 A CN102174403 A CN 102174403A CN 2011100370289 A CN2011100370289 A CN 2011100370289A CN 201110037028 A CN201110037028 A CN 201110037028A CN 102174403 A CN102174403 A CN 102174403A
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- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
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Abstract
The invention discloses application of a municipal domestic waste compost composite bacterial agent to improving drought resistance of lawn grass. The compost composite bacterial agent consists of liquid which is formed by diluting bacillus subtilis, actinomycetes and saccharomycetes in a volume ratio of 1 to 1 to 1 by 100 to 200 times. A result shows that drought damage of plants can be relieved to maintain a normal physiological metabolism function of the plants through activity of protective enzyme and osmotic adjustment substance content after diluted municipal domestic waste compost composite microbial bacterial agent is inoculated in the plants, so that the damage to the lawn plants caused by drought stress is effectively relieved, the drought resistance of the lawn plants is improved, and a basis is provided for planting of the lawn plants under a drought environment.
Description
Technical field
The invention belongs to environmental protection technical field, relating to the probiotics that extracts in the producing fertilizer from refuse in daily life is raw material, preparation highly effective compost composite fungus agent.The composite fungus agent of saying so more specifically improves the application of drought resistance of lawn plants in preparation.
Background technology
Composting (composting) is microorganism (bacterium, actinomycetes, fungi etc.) or the commercial strain that utilizes nature extensively to exist, controlledly promote biodegradable organism to stable soil ulmin (humicsubstance, HS) Biochemical processes of Zhuan Huaing.This process also is the part of earth surface ecological process, and it participates in the matter and energy circulation of earth surface.
Under artificial control condition, the organism in the solid waste can be transformed into organic fertilizer one compost (compost) by composting, this organic fertilizer is as the final product of composting, not only stable performance, and very little to the harm of environment.Therefore, composting is a solid waste, especially one of effective way of innoxious, stabilization of domestic waste and resource utilization.Composting development has been experienced very long history so far, and the historical process of its generation and development is looked back and analyzed, and can be to develop new composting process technology from now on reference and help in the theory and practice are provided.
Old generation and etc. (1989) adopt the rubbish substratum, in the producing fertilizer from refuse in daily life treating processes, when temperature reaches 45 ℃ and 55 ℃, the compost bacterial strain is separated, and find in the time of 45 ℃: aspergillus tubigensis [Aspergillus], bacillus [Bacillus], Pseudomonas [Pseudomonas] and Sporolactobacillus [Sporolactobacillus] etc. are the dominant microfloras in the compost.Then different during to 55 ℃, except bacillus [Bacillus] and Pseudomonas [Pseudomonas] still are the dominant microflora, lactobacillus genus [Lactobacillus], streptococcus [Streptococcus] and micromonospora [Micromonospora] belong to then becomes new dominant microflora, substituted other kind microorganism, show that in the composting process microbial population is among the running balance that constantly changes.
Isolate the microorganism of a large amount of participation high temperature aerobic compostings from domestic waste, be respectively bacillus, Pseudomonas through evaluation, lactobacillus genus, Staphylococcus, Escherichia etc. 5 belong to bacterium; Aspergillus, Mucor, monascus, Penicillium, Geotrichum, mould genus, arteries and veins sporeling genus, Cephalosporiums etc. 8 belong to mould; Streptomyces 1 belongs to actinomycetes; Saccharomycodes, fission yeast bacterium etc. 2 belong to yeast.This result of study has effectively promoted research of microorganism in the garbage compost and utilization.Hassen(2001) the biological investigation that changes is found to city compost process Ei, the high temperature that decomposing organic matter produces causes the important change of microflora, pathogenic bacterium, yeast and mesophilic bacteria are reduced in a large number, and sporiferous bacillus exists in a large number, in composting process, hot stage initial stage and Chinese stage bacterium are all in the highest flight.Marvin's ripples etc. has also been reported thermophilic fungus, and thermophilic brown actinomycetes etc. participate in the microbial population of garbage compost.
Organic composition from domestic waste, and decomposed utilization by Institute of Micro-biology.But various microorganisms are not quite similar to the capacity of decomposition and the decomposition rate of various materials, the microbial population that occurs in (middle temperature, high temperature) composting process under the differing temps and quantitatively also completely different.
Recent pertinent literature and actual production show: how to improve at present state of the art that producing fertilizer from refuse in daily life handles, improve processing efficiency, improve quality product, be the research contents that the related scientific research personnel are paid close attention to always; In compost material,, confirmed it is to accelerate compost maturity, improve the method for composting efficiency by a lot of institutes at some artificial microbial preparations of the autotelic interpolation of predetermined substance.Consider in a lot of researchs and production practice, the often more single bacterial strain of the composite fungus agent of rational proportion has more good effect, so how the various function stems of extracting in the compost and screening are combined exploitation highly effective compost composite fungus agent, fundamentally optimize with preparation rationally and use city compost matrix just to become the hot issue of related scientific research personnel's researchs.Although the effect study of plant microbe inoculation report is more, the microbiobacterial agent that most of research work relates to is only limited to from soil and extracts.Consumer garbage compostization is microorganism (bacterium, actinomycetes, fungi etc.) or the commercial strain that utilizes nature extensively to exist, controlledly promoting biodegradable organism to the Biochemical processes that stable humus transforms, is to be realized reclaiming organic waste, innoxious dynamic process by the very fast a plurality of microbial population actings in conjunction of structure of community succession.
Studies show that, single bacterium, fungi, actinomycetes colony, no matter how high its activity is, and the effect in accelerating the composting process is all less than the acting in conjunction of complex microbial community.People such as Ros and P é rez-Piqueres have studied the influence of inoculation inoculating microbe composite fungus agent to microorganism in the composting process, help understanding the Biochemical processes of composting process and adding the influence of microbial preparation to compost.Chinese scholars has been carried out series of theories and practical studies to the microorganism phenomenon in the composting process in recent years, people such as Vaz-Moreira analyze the community diversity of bacterium in the compost, in the hope of deeply being familiar with the essence of composting process, for the research of composting technology and technology is provided fundamental basis.But in the complex micro organism fungicide research process, evaluation and classification about the inner microbe species of compost, symbiosis and the relation of influencing each other have ambiguity and complicacy between the compost bacterial classification, and the compost microbe microbial inoculum is applied under the normal condition the aspect that influences to plant-growth, correlative study is also bright to be report.And about the evaluation and the classification of the inner microbe species of compost, symbiosis and the relation of influencing each other have ambiguity and complicacy between the compost bacterial classification, and from producing fertilizer from refuse in daily life, extract screening and the preparation compost is compounded with the beneficial microorganism microbial inoculum, the research that influences the aspect of lawn plant growth, physiological property be yet there are no report.
Drought stress is the important environmental factor of restriction arid and semi-arid regional plant g and D.Improving the adaptive faculty of plant in drought environment by inoculation foreign gene and synthetic microbial inoculum, is a hot fields of studying both at home and abroad in recent years.Microorganism can be set up symbiotic relationship with the plant more than 80%, and the foundation of symbiotic relationship often can improve the resistance of plant, promotes plant-growth; To this, existing scholar launches correlative study work.People such as He Xueli have studied the influence to growth of caragana microphylla Chinese Peashrub Root and drought resistance of AM fungi under the water stress condition.Song Rui bravely waits the people to utilize the synthetic microbiobacterial agent to improve crop anti-adversity, has proved that under the drought stress condition, the microorganism induction agent can promote growing of maize root system, improves the dry-matter accumulation of root system, thereby improves the drought-resistant ability of corn.Although the effect study of plant microbe inoculation report is more, the microbiobacterial agent that most of research work relates to is only limited to from soil and extracts.From producing fertilizer from refuse in daily life, extract and the preparation microbiobacterial agent, and the research that is applied to plant drought resistance yet there are no report.
Producing fertilizer from refuse in daily life is to be realized reclaiming organic waste, innoxious dynamic process by the very fast multiple microbial population acting in conjunction of structure of community succession.Multiple factors such as interaction in the compost between distribution of microbe population and population and compost organic substance composition and content, microorganism are closely related.People such as Gu Wenjie study inoculation external source microbial inoculum to microbe population in the compost and enzyme activity change, for the application of microbiobacterial agent and the improvement of composting process provide foundation.People such as Ivone analyze the community diversity of bacterium in the compost, in the hope of deeply being familiar with the essence of composting process, for composting technology and technical study are provided fundamental basis.In any case but, in the complex micro organism fungicide research process, about the compost microbe microbial inoculum yet there are no report to the correlative studys such as influence of plant-growth and physiological property.
Summary of the invention
The present invention as Inoculant, filters out suitable beneficial microbe strain with the compost microbe bacterial classification, and the complex micro organism fungicide that is mixed with different concns is inoculated in the turf establishment system.Test is carried out under medium drought is handled, complex micro organism fungicide by the research different concns is to the influence of rye grass and Festuca Arundinacea physiological and ecological characteristic, purpose is for screening good drought resisting compost complex micro organism fungicide, improve the lawn plant drought resistance, promote the planting of drought environment lawn plant that foundation is provided.
The invention provides following technical scheme for achieving the above object:
The highly effective compost composite fungus agent is in the application that improves drought resistance of lawn plants; Described compost composite fungus agent is a subtilis by volume ratio: the 3 bacterial classifications dilution 100-200 liquid doubly of actinomycetes: yeast=1:1:1 is formed.
Application of the present invention, wherein the colony number of 3 bacterial classifications is 2.44 * 10
9-2.47 * 10
9Between/the ml; The OD of grass genus bacillus
600Value is 0.567, and saccharomycetic OD value is 0.545.Wherein the lawn plant drought resistance refers to: mda content, superoxide-dismutase, peroxidase, catalase activity.Lawn plant wherein is rye grass and Festuca Arundinacea.
The present invention further discloses the highly effective compost composite fungus agent, wherein the screening method of 3 bacterial classifications is as follows:
(1) enrichment: the compost sample of gathering is taken by weighing 10 g place aseptic triangular flask, after adding the vibration evenly of 100 ml sterilized waters and granulated glass sphere, get 10ml suspension in the triangular flask that fills 100 ml enrichment mediums, respectively shaking table shaking culture 3 days under the temperature that is fit to;
(2) primary dcreening operation: after the nutrient solution after the enrichment carried out concentration gradient dilution, coat on the separation screening substratum, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony;
(3) multiple sieve: the inoculation that primary dcreening operation is obtained is cultivated under the condition of temperature that is fit to and shaking table speed in corresponding shake-flask culture base, is ordinate zou with the OD value, and incubation time is an X-coordinate, draws the growth curve of bacterial strain;
(4) close the preparation of microbiobacterial agent: on corresponding substratum, concrete steps are with the subtilis of screening, actinomycetes, yeast:
1. preserve the inclined-plane with stroke-physiological saline solution washing, shake up to the aseptic triangular flask bacteria suspension;
2. inoculate slant culture in the seed bottle, subtilis is cultivated 12h at 30 ℃, 180r/min and obtains seed liquor, and actinomycetes and yeast are cultivated 48 h and obtained seed liquor at 28 ℃, 220 r/min;
3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48h obtains fermented liquid in the fermentation flask.By volume ratio is a subtilis: actinomycetes: yeast=1:1:1 preparation composite microbial bacteria liquid.
Lawn plant of the present invention is rye grass and Festuca Arundinacea.Subtilis by volume wherein: actinomycetes: yeast=1:1:1 preparation.Wherein the composite fungus agent of Cai Yonging is the liquid of 200 times of dilutions.
The more detailed preparation method of the present invention is as follows:
What be illustrated especially is: subtilis (preserving number CCTCCM207094), actinomycetes (preserving number CPCC260118), yeast (preserving number CGMCCNO.1147) be by commercially available, but also can adopt following method to prepare 3 bacterial strains as required.The biochemical characteristic of 3 bacterial strains that it obtains is with commercially available identical.
The development materials and methods
The screening of bacterial strain
1. enrichment: the producing fertilizer from refuse in daily life sample of gathering is taken by weighing 10g place aseptic triangular flask, after adding the vibration evenly of 100mL sterilized water and granulated glass sphere, get 10mL suspension in the triangular flask that fills the 100mL enrichment medium, at 28 ℃, shaking culture 3-5d under 220 r/min.2. primary dcreening operation: after the nutrient solution after the enrichment carried out the concentration gradient dilution, coat on the separation screening substratum, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony.3. the drafting of growth curve: subtilis and yeast that primary dcreening operation is obtained are inoculated in the corresponding shake-flask culture base, respectively at 37 ℃ and 28 ℃, cultivate under the condition of 220 r/min, and be ordinate zou with the OD value under wavelength 600 nm and 560 nm respectively, incubation time is an X-coordinate, draws the growth curve of bacterial strain.
The preparation of producing fertilizer from refuse in daily life complex micro organism fungicide
Spawn culture: with subtilis, actinomycetes, the yeast enlarged culturing on corresponding substratum of screening, concrete steps: 1. preserve the inclined-plane with the stroke-physiological saline solution washing, shake up to the aseptic triangular flask bacteria suspension; 2. inoculate slant culture in the seed bottle, subtilis is at 30 ℃, and 180r/min cultivates 12 h and obtains seed liquor, and actinomycetes and yeast are at 28 ℃, and 220 r/min cultivate 48h and obtain seed liquor; 3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48h obtains fermented liquid in the fermentation flask, and composite microbial bacteria liquid is prepared by following processing.
Complex micro organism fungicide is used
Adopt the design of single factor randomized block experiment, limited resource is respectively: 1. drought stress, recording the soil maxmun field capacity is 43.54%.Medium drought is adopted in this test, and the degree of coercing is 55%-65% maxmun field capacity (MFC); 2. the complex micro organism fungicide of different concns is used, and sets processing mode under this drought stress condition: handle 1 for contrast, do not inoculate microbial inoculum (CK); Handle 2 and add original bacteria liquid (CM
1); Handle the microbial inoculum (CM of 100 times of diluents of 3 addings
2); Handle the microbial inoculum (CM of 200 times of diluents of 4 addings
3); Handle 5 and add 300 times of diluent microbial inoculum (CM
4); Handle 6 and add 400 times of diluent microbial inoculum (CM
5)
1.4 turf establishment
Select for use the more common English ryegrass of northern China (
Lolium perenneL.) and Festuca Arundinacea (
Festuca arundinaceaL.) be test materials.For examination lawn plant seed full seed, size evenly.Used soil is garden mould in the institute of Tianjin Normal University, and its physico-chemical property is: pH 7.44, saturation moisture content 0.58mL/g, organic matter 4.68%, full nitrogen 0.21%, available phosphorus 22.03 mg/kg, full potassium 45.61g/kg.After the collection, reject foreign material such as plant undesirable root and chad, cross the 2mm sieve, at 121 ℃ of 30 min that sterilize down, standby.Potted plant method is adopted in experiment, places sterile soil 200g in every culture vessel, and the 0.4g lawn seed is sowed on it.Unify quantitative water supply every day, water regime is preferably arranged, often change each culture vessel position, to guarantee the illumination unanimity to keep culture medium.When the plant strain growth height reaches 6-7cm, add the compost complex micro organism fungicide of different concns, behind the inoculation CM 3d, carry out drought stress again.Each handles 3 repetitions, and experiment is cultivated on the platform hothouse plants and carried out, and temperature is 20-25 ℃, and relative humidity is 30%-60%, and illumination is to penetrate indoor natural light.Carry out the mensuration of each index of plant behind the processing 40d.
Testing index
Gather fresh plant leaf, carry out the mensuration of following each index.The mensuration of proline content adopts the acid ninhydrine colorimetry; Mda (MDA) Determination on content adopts the thiobarbituricacid method; Guaiacol method is adopted in the POD determination of activity; The NBT method is adopted in the SOD determination of activity; Ultraviolet spectrophotometry is adopted in the CAT determination of activity; Soluble protein content adopts Xylene Brilliant Cyanine G G-250 staining to measure.
Percentage of water loss (%)=(heavy after fresh weight-dehydration)/fresh weight * 100%
The percentage of water loss height, the blade drying is described after dehydration more, i.e. leaf water content height, then excised leaf retention ability height.
Data analysis
Adopt Microsoft Excel 2003 and SPSS 11.5 softwares to handle.
Development results is analyzed
2.1 bacterial screening
Primary dcreening operation:, obtain the big and bacterium colony faster of growing of transparent circle/colony diameter ratio by the plate isolation screening.With subtilis, actinomycetes, the yeast of gained rule respectively separation, number and be preserved on the solid slant culture base.
Multiple sieve: by measuring subtilis and saccharomycetic growth curve (Fig. 1 and Fig. 2), analyze the highest and fecundity of growth activity preferably period be logarithmic phase, the bacterial classification of this phase is extracted, be diluted to different concns, be used for the lawn plant inoculation.As can be seen from Table 1, be rich in subtilis, actinomycetes and yeast in the gained microbiobacterial agent, the colony number of three bacterial classifications is 2.44 * 10
9-2.47 * 10
9Between, the OD of subtilis
600Value is 0.545, saccharomycetic OD
560Value is 0.567.
The colony number that table 1 microbiobacterial agent is rich in
2.2 CM is to the influence of lawn plant blade proline(Pro) and soluble protein content under the drought stress
Two kinds of lawn plant blade proline contents of complex micro organism fungicide processing all significantly are lower than nonvaccinated adjoining tree (Fig. 3) under the drought stress.Under 200 times of diluent composite fungus agents were handled, the Festuca Arundinacea proline content reduced at most, and comparison is according to having reduced by 30.1%.Under 100 times of diluent microbial inoculums were handled, the comparison of rye grass proline content was according to having reduced by 27.3%.And the treatment group under other weaker concn, lawn plant blade proline content compare with contrast that all there were significant differences (
P＜0.05).
As seen from Figure 4, the different concns complex micro organism fungicide has obviously reduced soluble protein content in two kinds of lawn plant blade cell.Under drought stress, soluble protein content is minimum under 200 times of diluents are handled in the Festuca Arundinacea cell, compared with the control, has reduced by 78.6%; And rye grass is minimum under 100 times of diluents are handled, and comparison is according to low by 66.2%, the inoculation microbial inoculum be described after, the intravital osmosis ability of vegetable cell strengthens, the raising of lawn plant drought-resistant ability.
CM is to the influence of lawn plant blade MDA content under the drought stress
Different concns compost complex micro organism fungicide is seen Fig. 5 to the influence of the following two kinds of lawn plant blade MDA content of drought stress.Compare with contrast, under the drought stress, inoculation compost complex micro organism fungicide has significantly reduced the MDA content of two kinds of lawn plant blades.Under high density, Festuca Arundinacea MDA content descends with the reduction of compost microbe bacteria suspension concentration, and content is minimum under 200 times of diluents are handled, and compares with contrast, has descended 24.3%; With the reduction of microbial bacteria agent concentration, MDA content shows the trend of increase again then.For rye grass; also show identical trend; different is; rye grass MDA content descends at most under 100 times of diluents; comparison is according to having reduced the influence of 42.4CM to lawn plant blade protection enzymic activity under the drought stress, and inoculation compost complex micro organism fungicide has significantly improved two lawn plant blade SOD, POD and CAT activity (table 2) under the drought stress.Under high density, along with the reduction of microbial bacteria agent concentration, 3 kinds of protective enzyme activity increase significantly, and when 200 times of diluents, Festuca Arundinacea blade SOD, POD and CAT activity reach the highest, are respectively 8.13,1.53 and 2.46 times of contrast; Composite fungus agent diluent multiple is 100 o'clock, and rye grass blade POD and CAT are active in the highest, and comparison is according to exceeding 64.4% and 56.1% respectively, and the SOD activity is 6.50 times of contrast; Then, along with the increase of extension rate, the protective enzyme activity begins again to descend.
CM is to the influence of lawn plant excised leaf retention ability under the drought stress
Inoculation different concns compost microbe microbial inoculum is seen Fig. 6 to the influence of the following two kinds of lawn plant excised leaf retention abilitys of drought stress.The microbe inoculation microbial inoculum is handled the excised leaf retention ability and all is higher than nonvaccinated contrast, and Festuca Arundinacea and rye grass reach peak value respectively when 200 and 100 extension rates, begins again then to descend, and the measurement result of this and other index matches.Rye grass is behind inoculation 100 diluents, and the retention ability comparison of excised leaf is according to having improved 7.00%.Illustrate that inoculation compost microbe microbial inoculum makes plant keep higher excised leaf retention ability, helps plant and improves the drought-resistant ability of self.
The development conclusion
Under drought stress, inoculation garbage compost complex micro organism fungicide can obviously improve lawn plant blade protection enzymic activity; suppress the synthetic of MDA; and keep higher excised leaf retention ability, thus alleviated the injury that drought stress brings, improved the drought resistance of plant.So at different lawn plants and growing environment, screening and cultivate advantage compost microbe bacterial classification and carry out artificial inoculation is the key that improves the lawn plant drought resistance, this is for the development of compost high-effect bacterial and use most important.
Description of drawings:
Fig. 1 is the growth curve of subtilis;
Fig. 2 is saccharomycetic growth curve;
Fig. 3 is the influence of CM to drought stress lawn plant blade proline content; With different lowercase alphabet differentials in the grass seeds data different significantly (P＜0.05); Down together;
Fig. 4 is the influence of CM to lawn plant blade soluble proteins under the drought stress;
Fig. 5 is the influence of CM to drought stress lawn plant blade MDA content;
Fig. 6 is the influence of CM to lawn plant excised leaf retention ability under the drought stress.
Embodiment
In order to explain enforcement of the present invention more fully, provide following preparation method's embodiment.These embodiments only are to explain rather than limit the scope of the invention.Subtilis, actinomycetes, yeast are by commercially available.But the also method of reference example 1 preparation.
(1) screening method of bacterial strain
Enrichment: the compost sample of gathering is taken by weighing 10g places aseptic triangular flask, add the vibration evenly of 100ml sterilized water and granulated glass sphere after, get 10ml suspension in the triangular flask that fills the 100ml enrichment medium, respectively shaking table shaking culture 3 days under the temperature that is fit to.
(2) primary dcreening operation: after the nutrient solution after the enrichment carried out concentration gradient dilution, coat on the separation screening substratum, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony.
(3) multiple sieve: the inoculation that primary dcreening operation is obtained is cultivated under the condition of temperature that is fit to and shaking table speed in corresponding shake-flask culture base, is ordinate zou with the OD value, and incubation time is an X-coordinate, draws the growth curve of bacterial strain.
Close the preparation of microbiobacterial agent:
Spawn culture: on corresponding substratum, concrete steps are with the subtilis, actinomycetes, yeast of screening:
1. preserve the inclined-plane with stroke-physiological saline solution washing, shake up to the aseptic triangular flask bacteria suspension;
2. inoculate slant culture in the seed bottle, subtilis is cultivated 12h at 30 ℃, 180r/min and obtains seed liquor, and actinomycetes and yeast are cultivated 48 h and obtained seed liquor at 28 ℃, 220 r/min; And be ordinate zou with the OD value under wavelength 600 nm and 560 nm respectively, incubation time is an X-coordinate, draws the growth curve of bacterial strain.
3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48 h obtain fermented liquid in the fermentation flask.Be subtilis by volume: actinomycetes: yeast=1:1:1 preparation composite microbial bacteria liquid, add-on is 10 mL.
Embodiment 2
Select for use the more common English ryegrass of northern China (
Lolium perenneL.) and Festuca Arundinacea (
Festuca arundinaceaL.) be test materials.For examination lawn plant seed full seed, size evenly.Used soil is garden mould in the institute of Tianjin Normal University, and its physico-chemical property is: pH 7.44, saturation moisture content 0.58mL/g, organic matter 4.68%, full nitrogen 0.21%, available phosphorus 22.03 mg/kg, full potassium 45.61g/kg.After the collection, reject foreign material such as plant undesirable root and chad, cross the 2mm sieve, at 121 ℃ of 30 min that sterilize down, standby.Potted plant method is adopted in experiment, places sterile soil 200g in every culture vessel, and the 0.4g lawn seed is sowed on it.Unify quantitative water supply every day, water regime is preferably arranged, often change each culture vessel position, to guarantee the illumination unanimity to keep culture medium.When the plant strain growth height reaches 7 cm, add the compost complex micro organism fungicide of different concns, subtilis: actinomycetes: yeast=1:1:1 (10 mL) (dilution 100-200 is doubly) behind the inoculation CM 3d, carries out drought stress again.Each handles 3 repetitions, and experiment is cultivated on the platform hothouse plants and carried out, and temperature is 25 ℃, and relative humidity is 30%%, and illumination is to penetrate indoor natural light.Carry out the mensuration of each index of plant behind the processing 40d.
Claims (4)
1. the producing fertilizer from refuse in daily life composite fungus agent is in the application that improves drought resistance of lawn plants; Described compost composite fungus agent is a subtilis by volume ratio: the 3 bacterial classifications dilution 100-200 liquid doubly of actinomycetes: yeast=1:1:1 is formed.
2. the described application of claim 1, wherein the colony number of 3 bacterial classifications is 2.44 * 10
9-2.47 * 10
9Between/the ml; The OD of grass genus bacillus
600Value is 0.567, saccharomycetic OD
560Value is 0.545.
3. the described application of claim 1, wherein the lawn plant drought resistance refers to: mda content, superoxide-dismutase, peroxidase, catalase activity.
4. the described application of claim 1, lawn plant wherein are rye grass and Festuca Arundinacea.
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| CN102599196A (en) * | 2012-03-02 | 2012-07-25 | 天津师范大学 | Tall fescue growth adjusting method of employing bacillus thuringiensis fermentation broth |
| CN104762233A (en) * | 2015-03-30 | 2015-07-08 | 天津师范大学 | Preparation method and application of lawn drought-resisting enhanced complex microbial community in garbage compost |
| CN104774769A (en) * | 2015-03-30 | 2015-07-15 | 天津师范大学 | Method for improving production performance of low temperature turf by adopting strengthened compost microbial agent |
| CN104789493A (en) * | 2015-03-30 | 2015-07-22 | 天津师范大学 | Method for improving protective enzyme activity of grass of water stress lawn by adopting reinforced drought-resistant microbial agent |
| CN104798818A (en) * | 2015-03-30 | 2015-07-29 | 天津师范大学 | Method for improving sod production drought resistance by adopting enhanced garbage compost microbial agent |
| CN104838841A (en) * | 2015-04-20 | 2015-08-19 | 天津师范大学 | Method adopting salt-tolerant reinforcement nanometer garbage compost for reinforcing turf grass salt resistance |
| CN104844346A (en) * | 2015-04-20 | 2015-08-19 | 天津师范大学 | Method for improving drought resistance of turf grass by adopting drought tolerance-reinforced active nanometer waste compost |
| CN104844345A (en) * | 2015-04-20 | 2015-08-19 | 天津师范大学 | Method for improving drought resistance of field turf by adopting drought tolerance-reinforced active nanometer waste compost |
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