CN102174441B - Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass - Google Patents

Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass Download PDF

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CN102174441B
CN102174441B CN2011100370575A CN201110037057A CN102174441B CN 102174441 B CN102174441 B CN 102174441B CN 2011100370575 A CN2011100370575 A CN 2011100370575A CN 201110037057 A CN201110037057 A CN 201110037057A CN 102174441 B CN102174441 B CN 102174441B
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chlorophyll
actinomycetes
subtilis
bacterial agent
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CN102174441A (en
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多立安
赵树兰
王晶晶
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Tianjin Normal University
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Abstract

The invention discloses application of a prepared domestic waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn plants and promoting synthesis of chlorophyll. The composite bacterial agent consists of three strains, namely bacillus subtilis, actinomycetes and saccharomycetes in volume ratio of 1 to 1 to 1. Experiments show that seed germination rate, seedling height, aboveground dry weight, root weight, chlorophyll a content and chlorophyll b content of festuca arundinacea are 13.41 percent, 58.92 percent, 72.86 percent, 100 percent, 68.92 percent and 37.61 percent higher than contrast values respectively and the seed germination rate, the seedling height, the aboveground dry weight, the root weight, the chlorophyll a content and the chlorophyll b content of ryegrass are 15.11 percent, 34.44 percent, 71.74 percent, 100 percent, 66.89 percent and 33.41 percent higher than contrast values respectively after being treated by inoculating the three-strain composite bacterial agent. Technical effects show that the high-efficiency compost composite bacterial agent provides proper growth conditions and nutrients for plants, meets requirements on growth conditions required by the plants and therefore contributes to growth of the ryegrass and the festuca arundinacea.

Description

The application of garbage compost composite fungus agent aspect enhancing turfgrass photosynthetic capacity
Technical field
The invention belongs to environmental protection technical field, relating to the probiotics that extracts in the producing fertilizer from refuse in daily life is raw material, preparation highly effective compost composite fungus agent.The preparation composite fungus agent say so more specifically strengthening the lawn plant photosynthetic capacity, promote the application of the synthetic aspect of chlorophyll.
Background technology
Composting (composting) is mikrobe (bacterium, actinomycetes, fungi etc.) or the commercial strain that utilizes nature extensively to exist; Controlledly promote biodegradable organism to stable soil ulmin (humicsubstance, the Biochemical processes that HS) transform.This process also is the part of earth surface ecological process, and it participates in the matter and energy circulation of earth surface.
Under artificial control condition, can the organism in the solid waste be transformed into organic fertilizer one compost (compost) through composting, this organic fertilizer is as the final product of composting, not only stable performance, and very little to the harm of environment.Therefore, composting is a solid waste, especially one of effective way of innoxious, stabilization of domestic waste and resource utilization.Composting development has been experienced very long history so far, and the historical process of its generation and development is looked back and analyzed, and can be to develop new composting process technology from now on reference and help in the theory and practice are provided.
Old generation and etc. (1989) adopt the rubbish substratum; In the producing fertilizer from refuse in daily life treating processes; When temperature reaches 45 ℃ and 55 ℃; The compost bacterial strain is separated, and find in the time of 45 ℃: aspergillus tubigensis [Aspergillus], bacillus [Bacillus], Pseudomonas [Pseudomonas] and Sporolactobacillus [Sporolactobacillus] etc. are the dominant microfloras in the compost.Then different during to 55 ℃; Except bacillus [Bacillus] and Pseudomonas [Pseudomonas] still are the dominant microflora; Lactobacillus genus [Lactobacillus], streptococcus [Streptococcus] and micromonospora [Micromonospora] belong to then becomes new dominant microflora; Substituted other kind mikrobe, be illustrated in the composting process, microbial population is among the running balance that constantly changes.
From domestic waste, isolate the mikrobe of a large amount of participation high temperature aerobic compostings, be respectively bacillus, Pseudomonas through identifying, lactobacillus genus, Staphylococcus, Escherichia etc. 5 belong to bacterium; Aspergillus, Mucor, monascus, penicillium, Geotrichum, mould genus, arteries and veins sporeling genus, Cephalosporiums etc. 8 belong to mould; Streptomyces 1 belongs to actinomycetes; Saccharomycodes, fission yeast bacterium etc. 2 belong to yeast.This result of study has effectively promoted research of mikrobe in the garbage compost and utilization.Hassen (2001) is to the biological investigation discovery that changes of city compost process Ei; The high temperature that decomposing organic matter produces causes the significant variations of microflora; Pathogenic bacterium, yeast and mesophilic bacteria are reduced in a large number; And sporiferous bacillus exists in a large number, and in composting process, hot stage initial stage and Chinese stage bacterium are all in the highest flight.Marvin's ripples etc. has also been reported thermophilic fungus, and thermophilic brown actinomycetes etc. are participated in the microbial population of garbage compost.
Organic composition from domestic waste, and decomposed utilization by Institute of Micro-biology.But various mikrobes are not quite similar to the resolving power and the decomposition rate of various materials, the microbial population that occurs in (middle temperature, high temperature) composting process under the differing temps and quantitatively also completely different.
Recent pertinent literature and actual production show: how to improve producing fertilizer from refuse in daily life Treatment Technology level at present, improve processing efficiency, improve quality product, be the research contents that the related scientific research personnel are paid close attention to always; In compost material,, confirmed it is to accelerate compost maturity, improve the method for composting efficiency by a lot of institutes to some artificial microbial preparations of the autotelic interpolation of predetermined substance.Consider in a lot of researchs and production practice; The often more single bacterial strain of the composite fungus agent of rational proportion has more good effect; So how the various function stems of extracting in the compost and screening are combined exploitation highly effective compost composite fungus agent, fundamentally optimize with preparation rationally and use city compost matrix just to become the hot issue of related scientific research personnel's researchs.Although the effect study of plant microbe inoculation report is more, the microbiobacterial agent that most of research work relates to is only limited to from soil and extracts.Consumer garbage compostization is mikrobe (bacterium, actinomycetes, fungi etc.) or the commercial strain that utilizes nature extensively to exist; Controlledly promoting biodegradable organism to the Biochemical processes that stable humus transforms, is to be realized reclaiming organic waste, innoxious dynamic process by the very fast a plurality of microbial population actings in conjunction of structure of community succession.
Research shows, single bacterium, fungi, actinomycetes colony, and no matter how high its activity is, and the effect in accelerating the composting process is all less than the acting in conjunction of complex microbial community.People such as Ros and P é rez-Piqueres have studied the influence of inoculation inoculating microbe composite fungus agent to mikrobe in the composting process, help understanding the Biochemical processes of composting process and adding the influence of microbial preparation to compost.Chinese scholars has been carried out series of theories and practical studies to the mikrobe phenomenon in the composting process in recent years; People such as Vaz-Moreira analyze the community diversity of bacterium in the compost; In the hope of deeply being familiar with the essence of composting process, for the research of composting technology and technology is provided fundamental basis.But in the complex micro organism fungicide research process; Evaluation and classification about the inner microbe species of compost; Symbiosis and the relation of influencing each other have ambiguity and complicacy between the compost bacterial classification; And the compost microbe microbial inoculum is applied under the normal condition aspect that influences to plant-growth, correlative study is also bright to be report.And about the evaluation and the classification of the inner microbe species of compost; Symbiosis and the relation of influencing each other have ambiguity and complicacy between the compost bacterial classification; And from producing fertilizer from refuse in daily life, extract screening and the preparation compost is compounded with the beneficial microorganism microbial inoculum, the research that influences the aspect of lawn plant growth, physiological property is not also appeared in the newspapers.
Summary of the invention
The present invention is through extracting and identify the mikrobe in the consumer garbage compost; Filter out suitable beneficial microbe strain; Be mixed with the complex micro organism fungicide of the microbiobacterial agent of single bacterial classification, two bacterial classification microbiobacterial agent and 3 bacterial classifications; Explore of the influence of each compost microbe microbial inoculum, for the compost microbe microbial inoculum provides scientific basis in turf establishment interim application just to seed germination of lawn plants and primary growth.
For realizing that above-mentioned purpose the invention provides following technical scheme:
A kind of highly effective compost composite fungus agent, it is characterized in that it is a subtilis by volume ratio: 3 bacterial classifications of actinomycetes: yeast=1:1:1 are formed.
Composite fungus agent of the present invention, wherein the colony count of 3 bacterial classifications is 2.44 * 10 9-2.47 * 10 9Between/the ml; The OD of grass genus bacillus 600Value is 0.567, saccharomycetic OD 560Value is 0.545.
The present invention further discloses the highly effective compost composite fungus agent, wherein the screening method of 3 bacterial classifications is following:
(1) enrichment: the compost sample of gathering is taken by weighing 10g place aseptic triangular flask; After adding the vibration evenly of 100ml sterilized water and granulated glass sphere; Get 10ml suspension-s in the triangular flask that fills the 100ml enrichment medium, respectively shaking table shaking culture 3 days under the temperature that is fit to;
(2) primary dcreening operation: after the nutrient solution after the enrichment carried out concentration gradient dilution, coat on the separation screening substratum, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony;
(3) multiple sieve: the inoculation that primary dcreening operation is obtained is cultivated under the condition of temperature that is fit to and shaking table speed in corresponding shake-flask culture base, is ordinate zou with the OD value, and incubation time is an X-coordinate, the growth curve of drafting bacterial strain;
(4) close the preparation of microbiobacterial agent: on corresponding substratum, concrete steps are with the subtilis of screening, actinomycetes, yeast:
1. preserve the inclined-plane with SPSS washing, shake up to the aseptic triangular flask bacteria suspension;
2. inoculate slant culture in the seed bottle, subtilis is cultivated 12h at 30 ℃, 180r/min and obtains seed liquor, and actinomycetes and yeast are cultivated 48 h and obtained seed liquor at 28 ℃, 220 r/min;
3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48 h obtain fermented liquid in the fermentation flask.By volume ratio is a subtilis: actinomycetes: yeast=1:1:1 preparation composite microbial bacteria liquid.
The present invention further discloses the preparation composite fungus agent again strengthening the lawn plant photosynthetic capacity, promotes the application of the synthetic aspect of chlorophyll.Wherein the lawn plant photosynthetic capacity refers to: rate of emergence, seedling plant height, ground dry weight, root weigh.
Lawn plant of the present invention is rye grass and Festuca Arundinacea.Subtilis by volume wherein: actinomycetes: the composite microbial bacteria liquid add-on of yeast=1:1:1 preparation is 10ml, can add 2-3 time as required.
The more detailed preparation method of the present invention is following:
Special explain be: subtilis (preserving number CCTCCM207094), actinomycetes (preserving number CPCC260118), yeast (preserving number CGMCCNO.1147) be by commercially available, but also can adopt following method to prepare 3 bacterial strains as required.The biochemical characteristic of 3 bacterial strains that it obtains is with commercially available identical.
1 development materials and methods
1.1 material
Select for use the more common English ryegrass of northern China ( Lolium perenneL.) (kind is Paribaldi, from Denmark) and Festuca Arundinacea ( Festuca arundinaceaL.) (kind is Acending Star, from the U.S.) is test materials.
Method
1.2.1The screening method of bacterial strain
Enrichment: the compost sample of gathering is taken by weighing 10g place aseptic triangular flask; After adding the vibration evenly of 100ml sterilized water and granulated glass sphere; Get 10ml suspension-s in the triangular flask that fills the 100ml enrichment medium, respectively shaking table shaking culture 3-5 days under the temperature that is fit to.
Primary dcreening operation: after the nutrient solution after the enrichment carried out concentration gradient dilution, coat on the separation screening substratum, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony.
Multiple sieve: the inoculation that primary dcreening operation is obtained is cultivated under the condition of temperature that is fit to and shaking table speed in corresponding shake-flask culture base, is ordinate zou with the OD value, and incubation time is an X-coordinate, the growth curve of drafting bacterial strain.
The preparation of complex micro organism fungicide
Spawn culture: with subtilis, actinomycetes, the yeast enlarged culturing on corresponding substratum of screening, concrete steps are: 1. preserve the inclined-plane with the SPSS washing, shake up to the aseptic triangular flask bacteria suspension;
2. inoculate slant culture in the seed bottle, subtilis is cultivated 12 h and obtains seed liquor at 30 ℃, 180r/min, and actinomycetes and yeast are cultivated 48 h and obtained seed liquor at 28 ℃, 220 r/min;
3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48 h obtain fermented liquid in the fermentation flask.By following processing preparation composite microbial bacteria liquid.
The application of turf establishment and microbiobacterial agent
Table 1 microbiobacterial agent inoculation different treatment
Different treatment Subtilis Yeast Actinomycetes
Ⅰ(CK) 0 ml 0 ml 0 ml
10 ml
10 ml
10 ml
5 ml 5 ml
5 ml 5 ml
5 ml 5 ml
3.33 ml 3.33 ml 3.33 ml
The lawn plant seed full seed of selecting for use, size are evenly.Used soil is garden mould in the institute of Tianjin Normal University, and its physico-chemical property is: pH 7.44, saturation moisture content 0.58ml/g, organic matter 4.68%, full nitrogen 0.21%, available phosphorus 22.03 mg/kg, full potassium 45.61g/kg.After the collection, reject foreign material such as plant undesirable root and chad, cross the 2mm sieve, at 121 ℃ of 30min that sterilize down, subsequent use.Rye grass and tall fescue seed are sowed at (diameter 9cm) in the petridish that fills sterile soil 60g, 150 in every ware respectively.
The sowing of fixing grain number, purity 100%; Seed germination rate about 85%.Unify quantitative water supply every day, water regime is preferably arranged, often change each culture vessel position to keep culture medium, consistent to guarantee illumination.Treat plant-growth time the tillering phase, add the compost microbe microbial inoculum of the said different treatment of table 1, each handles 3 repetitions.Test is cultivated on the platform hothouse plants and is carried out, and temperature is 15-20 ℃, and relative humidity is 40%-55%, and illumination is to penetrate indoor natural light, continues to handle each index of measuring plant after 35 days.
The mensuration of index
Sow 3d and begin to add up seed germination quantity; Sprout stable back 8d, measure percentage of germination.
Percentage of germination=(subnumber is planted experimentally in chitting piece number/confession) * 100%.
Between the lawn plant during cultivation, after the inoculation microbial inoculum was handled a week, per mensuration of carrying out a plant height in 5 days cradled after the inoculation microbial inoculum is handled 35d, measures overground part dry weight, weight of root system and seedling chlorophyll content respectively.The mensuration of chlorophyll content adopts spectrophotometry.
Data analysis
Data analysis adopts EXCEL and SPSS11.5 analysis software to handle.
2 development results analyses
2.1 bacterial screening
Primary dcreening operation:, obtain the big and bacterium colony faster of growing of transparent circle/colony diameter ratio through the plate isolation screening.With subtilis, actinomycetes, the yeast of gained rule respectively separation, number and be preserved on the solid slant culture base.
Multiple sieve: through measuring subtilis and saccharomycetic growth curve (Fig. 1 and Fig. 2); Analyze the growth activity the highest with prolificacy preferably period be logarithmic phase; The bacterial classification of this phase is extracted, be made into different treatment (table 1), be used for the lawn plant inoculation.Can find out that by table 2 be rich in subtilis, actinomycetes and yeast in the gained microbiobacterial agent, the colony count of 3 bacterial classifications is 2.44 * 10 9-2.47 * 10 9Between/the ml, the OD of subtilis 600Value is 0.567, saccharomycetic OD 560Value is 0.545.
The colony count that table 2 microbiobacterial agent is rich in
Microbial inoculum 1ml colony count (number/ml) 10ml colony count (number/10 ml) The OD value
Subtilis (2.468±0.03)×10 9 (2.468±0.03)×10 10 0.567±0.001
Actinomycetes (2.435±0.083)×10 9 (2.435±0.08)×10 10 -
Yeast (2.447±0.07)×10 9 (2.463±0.07)×10 10 0.545±0.001
2.2 microbiobacterial agent is to the influence of rye grass and tall fescue seed percentage of germination and growth of seedling
Can significantly promote rye grass and tall fescue seed to germinate and growth of seedling (table 3) after the inoculation microbial inoculum; Different microbial inoculums compare between handling; The processing that shows 3 kinds of microbial strainss of inoculation is better than the processing of the two bacterial classifications of inoculation, and the processing of the two bacterial classifications of inoculation is better than the processing of inoculating single bacterial classification.Under the complex micro organism fungicide (VIII) of 3 bacterial classifications was handled, the tall fescue seed percentage of germination was compared according to significantly raising, and comparison is shone and exceeded 13.41%, and the rye grass comparison is according to exceeding 15.11%.Festuca Arundinacea and rye grass plant height are handled down at inoculation 3 bacterial classifications, and have significant difference between contrasting ( P<0.05), exceed 58.92% and 34.44% respectively.Total dry weight from Festuca Arundinacea and rye grass; Comparison is according to having increased by 72.86% and 71.74% respectively; Explain that microbial inoculum is that plant provides suitable growth conditions and nutritive ingredient, has satisfied the required upgrowth situation of plant, thereby has helped the growth of rye grass and Festuca Arundinacea.
The different microbial inoculums of table 3 are handled the influence to lawn plant percentage of germination, plant height and ground biomass
Figure 2011100370575100002DEST_PATH_IMAGE001
With different lowercase alphabet differentials in the grass seeds different treatment data different significantly ( P<0.05); Down together
2.3 microbiobacterial agent is to the influence of rye grass and Festuca Arundinacea root growth
The microbial inoculum of different treatment can obviously promote the growth (table 4) of rye grass and Festuca Arundinacea root system, handles the most remarkable with the complex micro organism fungicide (VIII) of inoculating 3 bacterial classifications.The Festuca Arundinacea root is long, root is heavy, the fibrous root number is higher than contrast 62.71%, 100%, 65.78% respectively ( P<0.05).
Under the complex micro organism fungicide (VIII) of 3 bacterial classifications was handled, the rye grass roots was long, root is heavy, the fibrous root number is respectively 1.41,2 and 1.66 times that contrast.The microbe inoculation microbial inoculum has increased the root/shoot ratio of rye grass and Festuca Arundinacea, explains that microbial inoculum is more obvious to the promoter action of these two kinds of lawn plant root growths.
The different microbial inoculums of table 4 are to the influence of lawn plant root growth
Figure 2011100370575100002DEST_PATH_IMAGE002
2.4 microbiobacterial agent is to rye grass and Festuca Arundinacea Chlorophyll of Leaves
Different microbial inoculums are handled the raising (Fig. 3) that helps rye grass and Festuca Arundinacea chlorophyll content.The processing that shows 3 kinds of microbial strainss of inoculation equally is higher than the processing of the two bacterial classifications of inoculation, and the processing of the two bacterial classifications of inoculation is higher than the processing of inoculating single bacterial classification.Handle down at the complex micro organism fungicide (VIII) of 3 bacterial classifications, rye grass and Festuca Arundinacea chlorophyll content be for the highest, Festuca Arundinacea chlorophyll a and chlorophyll b content exceed respectively and contrast 68.92% and 37.61% ( P<0.05); Rye grass exceed respectively the contrast 66.89% and 33.41% ( P<0.05).Explain that the microbe inoculation microbial inoculum has strengthened the photosynthetic capacity of plant, help chlorophyllous synthetic.
3 development conclusions
The compost microbe microbial inoculum is applied to rye grass and the planting of Festuca Arundinacea initial stage, can obviously promotes this two kinds of lawn plant growths, improve photosynthetic efficiency, promote chlorophyllous synthetic.And after inoculating the complex micro organism fungicide of 3 bacterial classifications, plant strain growth ability, nutritional status and chlorophyllous content all are superior to other treatment group, and this helps improving derive nutrients and the energy for growth of plant.Comprehensive each item index considers that the conclusion of this research is that the optimum compound proportion example of compost useful microbe bacterial classification is subtilis: actinomycetes: yeast=1:1:1.
Description of drawings:
Fig. 1 is the growth curve of subtilis;
Fig. 2 is saccharomycetic growth curve;
Fig. 3, Fig. 4 are the influences of different microbial inoculums to the lawn plant chlorophyll content.
Embodiment
In order to explain enforcement of the present invention more fully, following preparing method's embodiment is provided.These embodiments only are to explain rather than limit scope of the present invention.Subtilis, actinomycetes, yeast are by commercially available.But the also method preparation of reference implementation example 1.
Embodiment 1
The screening method of 3 bacterial classifications is following:
(1) enrichment: the compost sample of gathering is taken by weighing 10g place aseptic triangular flask; After adding the vibration evenly of 100ml sterilized water and granulated glass sphere; Get 10ml suspension-s in the triangular flask that fills the 100ml enrichment medium, respectively shaking table shaking culture 3 days under the temperature that is fit to;
(2) primary dcreening operation: after the nutrient solution after the enrichment carried out concentration gradient dilution, coat on the separation screening substratum, be inverted constant temperature culture, observe the size of transparent circle, choose transparent circle/colony diameter ratio and carry out multiple sieve than the bacterial strain of macrocolony;
(3) multiple sieve: the inoculation that primary dcreening operation is obtained is cultivated under the condition of temperature that is fit to and shaking table speed in corresponding shake-flask culture base, is ordinate zou with the OD value, and incubation time is an X-coordinate, the growth curve of drafting bacterial strain;
(4) close the preparation of microbiobacterial agent: on corresponding substratum, concrete steps are with the subtilis of screening, actinomycetes, yeast:
1. preserve the inclined-plane with SPSS washing, shake up to the aseptic triangular flask bacteria suspension;
2. inoculate slant culture in the seed bottle, subtilis is cultivated 12h at 30 ℃, 180r/min and obtains seed liquor, and actinomycetes and yeast are cultivated 48 h and obtained seed liquor at 28 ℃, 220 r/min;
3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48h obtains fermented liquid in the fermentation flask.By volume ratio is a subtilis: actinomycetes: yeast=1:1:1 preparation composite microbial bacteria liquid.
Embodiment 2
The lawn plant seed full seed of selecting for use, size are evenly.Used soil is garden mould in the institute of Tianjin Normal University, and its physico-chemical property is: pH 7.44, saturation moisture content 0.58 ml/g, organic matter 4.68%, full nitrogen 0.21%, available phosphorus 22.03 mg/kg, full potassium 45.61g/kg.After the collection, reject foreign material such as plant undesirable root and chad, cross the 2mm sieve, at 121 ℃ of 30min that sterilize down, subsequent use.Rye grass and tall fescue seed are sowed at (diameter 9cm) in the petridish that fills sterile soil 60g, 150 in every ware respectively.The sowing of fixing grain number, purity 100%; Seed germination rate about 85%.Unify quantitative water supply every day, water regime is preferably arranged, often change each culture vessel position to keep culture medium, consistent to guarantee illumination.Treat plant-growth time the tillering phase, by volume ratio is a subtilis: actinomycetes: yeast=1:1:1 adds compost microbe microbial inoculum (10ml, 3 times repeat).Test is cultivated on the platform hothouse plants and is carried out, and temperature is 15 ℃, and relative humidity is 55%, and illumination is to penetrate indoor natural light, continues to handle each index of measuring plant after 35 days.

Claims (2)

1. preparation consumer garbage compost composite fungus agent promotes the application of chlorophyll aspect synthesizing strengthening rye grass and Festuca Arundinacea photosynthetic capacity; Wherein said consumer garbage compost composite fungus agent is that the fermented liquid by subtilis CCTCC M207094, actinomycetes CPCC 260118 and three kinds of bacterium of yeast CGMCC NO.1147 is that 1:1:1 is formulated with the volume ratio; The colony count of said 3 bacterial classifications is 2.44 * 10 9-2.47 * 10 9Between/the ml, the OD of subtilis 600Value is 0.567, saccharomycetic OD 560Value is 0.545; Wherein the chlorophyll content that refers to of rye grass and Festuca Arundinacea photosynthetic capacity, seedling plant height, dry weight, root are heavy on the ground; The preparation method of said consumer garbage compost composite fungus agent is following:
1. preserve the inclined-plane with SPSS washing, shake up to the aseptic triangular flask bacteria suspension;
2. inoculate slant culture in the seed bottle, subtilis is cultivated 12h at 30 ℃, 180r/min and obtains seed liquor, and actinomycetes and yeast are cultivated 48 h and obtained seed liquor at 28 ℃, 220 r/min;
3. the seed liquor of concentration 10% is inoculated into that enlarged culturing 48 h obtain fermented liquid in the fermentation flask; By volume ratio is a subtilis: actinomycetes: yeast=1:1:1 preparation composite microbial bacteria liquid.
2. the described application of claim 1, wherein compost composite fungus agent add-on is 10ml, adds 2-3 time.
CN2011100370575A 2011-02-14 2011-02-14 Application of waste compost composite bacterial agent to enhancing photosynthetic capacity of lawn grass Expired - Fee Related CN102174441B (en)

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CN112625950B (en) * 2020-12-21 2021-09-14 青岛尚德生物技术有限公司 Green increasing microbial inoculum for increasing chlorophyll content of green leaf vegetables and preparation method and application thereof

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
Ivone Vaz-moreira.Diversity of bacterial isolates from commericial and homemade composts.《Microbial ecology》.2007,第55卷(第4期),714-722. *
M.ROS等.Hydrolase activities,microbial biomass and bacterial community in a soil after long-term amendment with different composts.《Soil biology and biochemistry》.2006,第38卷(第12期),3443-3452. *
M.ROS等.Hydrolaseactivities microbial biomass and bacterial community in a soil after long-term amendment with different composts.《Soil biology and biochemistry》.2006
解开治 等.接种微生物菌剂对猪粪堆肥过程中细菌群落多样性的影响.《应用生态学报》.2009,第20卷(第8期),2012-2018. *

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