CN115851465A - Composite fermentation inoculant, method for preparing fermented rapeseed meal for feed and application of composite fermentation inoculant - Google Patents
Composite fermentation inoculant, method for preparing fermented rapeseed meal for feed and application of composite fermentation inoculant Download PDFInfo
- Publication number
- CN115851465A CN115851465A CN202211608483.4A CN202211608483A CN115851465A CN 115851465 A CN115851465 A CN 115851465A CN 202211608483 A CN202211608483 A CN 202211608483A CN 115851465 A CN115851465 A CN 115851465A
- Authority
- CN
- China
- Prior art keywords
- fermentation
- rapeseed meal
- parts
- aspergillus niger
- fermented
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
Links
- 238000000855 fermentation Methods 0.000 title claims abstract description 97
- 230000004151 fermentation Effects 0.000 title claims abstract description 97
- 235000019779 Rapeseed Meal Nutrition 0.000 title claims abstract description 93
- 239000004456 rapeseed meal Substances 0.000 title claims abstract description 93
- 239000002054 inoculum Substances 0.000 title claims abstract description 44
- 238000000034 method Methods 0.000 title claims abstract description 18
- 239000002131 composite material Substances 0.000 title claims abstract description 16
- 241000228245 Aspergillus niger Species 0.000 claims abstract description 36
- 108010059892 Cellulase Proteins 0.000 claims abstract description 35
- 229940106157 cellulase Drugs 0.000 claims abstract description 35
- 241000193749 Bacillus coagulans Species 0.000 claims abstract description 27
- 241000191998 Pediococcus acidilactici Species 0.000 claims abstract description 27
- 241000223254 Rhodotorula mucilaginosa Species 0.000 claims abstract description 27
- 229940054340 bacillus coagulans Drugs 0.000 claims abstract description 27
- 150000001875 compounds Chemical class 0.000 claims abstract description 8
- 239000002068 microbial inoculum Substances 0.000 claims abstract description 7
- 238000002156 mixing Methods 0.000 claims description 25
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 20
- 238000001035 drying Methods 0.000 claims description 16
- 230000029058 respiratory gaseous exchange Effects 0.000 claims description 16
- 238000007873 sieving Methods 0.000 claims description 16
- 230000000694 effects Effects 0.000 claims description 11
- 241000894006 Bacteria Species 0.000 claims description 6
- 235000013527 bean curd Nutrition 0.000 claims description 6
- 235000021107 fermented food Nutrition 0.000 claims description 6
- 239000000203 mixture Substances 0.000 claims description 3
- 235000021110 pickles Nutrition 0.000 claims description 3
- 238000002360 preparation method Methods 0.000 claims description 2
- 230000000433 anti-nutritional effect Effects 0.000 abstract description 10
- 108090000623 proteins and genes Proteins 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
- 235000019750 Crude protein Nutrition 0.000 abstract description 5
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 abstract description 5
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 abstract description 5
- 125000004383 glucosinolate group Chemical group 0.000 abstract description 5
- 235000016709 nutrition Nutrition 0.000 abstract description 5
- 229940068041 phytic acid Drugs 0.000 abstract description 5
- 235000002949 phytic acid Nutrition 0.000 abstract description 5
- 239000000467 phytic acid Substances 0.000 abstract description 5
- 235000018553 tannin Nutrition 0.000 abstract description 5
- 229920001864 tannin Polymers 0.000 abstract description 5
- 239000001648 tannin Substances 0.000 abstract description 5
- 241001465754 Metazoa Species 0.000 abstract description 4
- 239000000835 fiber Substances 0.000 abstract description 4
- 239000003205 fragrance Substances 0.000 abstract description 2
- 238000004519 manufacturing process Methods 0.000 abstract description 2
- 244000144972 livestock Species 0.000 abstract 1
- 244000144977 poultry Species 0.000 abstract 1
- 239000003795 chemical substances by application Substances 0.000 description 18
- 239000000047 product Substances 0.000 description 15
- 238000007789 sealing Methods 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 13
- 238000003756 stirring Methods 0.000 description 10
- 241001125889 Micropterus salmoides Species 0.000 description 7
- 241000276707 Tilapia Species 0.000 description 7
- 235000019733 Fish meal Nutrition 0.000 description 6
- 239000004467 fishmeal Substances 0.000 description 6
- 235000019764 Soybean Meal Nutrition 0.000 description 5
- 239000004455 soybean meal Substances 0.000 description 5
- 238000009360 aquaculture Methods 0.000 description 3
- 244000144974 aquaculture Species 0.000 description 3
- 230000000813 microbial effect Effects 0.000 description 3
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 2
- 230000002411 adverse Effects 0.000 description 2
- 150000001413 amino acids Chemical class 0.000 description 2
- 230000008901 benefit Effects 0.000 description 2
- 230000037396 body weight Effects 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 150000002540 isothiocyanates Chemical class 0.000 description 2
- 235000021049 nutrient content Nutrition 0.000 description 2
- 230000001737 promoting effect Effects 0.000 description 2
- 238000005070 sampling Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- 230000004584 weight gain Effects 0.000 description 2
- 235000019786 weight gain Nutrition 0.000 description 2
- 244000063299 Bacillus subtilis Species 0.000 description 1
- 235000014469 Bacillus subtilis Nutrition 0.000 description 1
- 240000002791 Brassica napus Species 0.000 description 1
- 235000004977 Brassica sinapistrum Nutrition 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 244000068988 Glycine max Species 0.000 description 1
- 235000010469 Glycine max Nutrition 0.000 description 1
- KDXKERNSBIXSRK-YFKPBYRVSA-N L-lysine Chemical compound NCCCC[C@H](N)C(O)=O KDXKERNSBIXSRK-YFKPBYRVSA-N 0.000 description 1
- 241000186660 Lactobacillus Species 0.000 description 1
- 241000269794 Lateolabrax Species 0.000 description 1
- 239000004472 Lysine Substances 0.000 description 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 description 1
- 241000235395 Mucor Species 0.000 description 1
- 241000276701 Oreochromis mossambicus Species 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 235000014680 Saccharomyces cerevisiae Nutrition 0.000 description 1
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000000975 bioactive effect Effects 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 239000006227 byproduct Substances 0.000 description 1
- 229940088598 enzyme Drugs 0.000 description 1
- 239000005556 hormone Substances 0.000 description 1
- 229940088597 hormone Drugs 0.000 description 1
- 230000008676 import Effects 0.000 description 1
- 229910052742 iron Inorganic materials 0.000 description 1
- 235000021109 kimchi Nutrition 0.000 description 1
- 229940039696 lactobacillus Drugs 0.000 description 1
- WPBNNNQJVZRUHP-UHFFFAOYSA-L manganese(2+);methyl n-[[2-(methoxycarbonylcarbamothioylamino)phenyl]carbamothioyl]carbamate;n-[2-(sulfidocarbothioylamino)ethyl]carbamodithioate Chemical compound [Mn+2].[S-]C(=S)NCCNC([S-])=S.COC(=O)NC(=S)NC1=CC=CC=C1NC(=S)NC(=O)OC WPBNNNQJVZRUHP-UHFFFAOYSA-L 0.000 description 1
- 230000002503 metabolic effect Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000019629 palatability Nutrition 0.000 description 1
- 231100000614 poison Toxicity 0.000 description 1
- 238000003825 pressing Methods 0.000 description 1
- 239000006041 probiotic Substances 0.000 description 1
- 235000018291 probiotics Nutrition 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 108090000765 processed proteins & peptides Proteins 0.000 description 1
- 229910052711 selenium Inorganic materials 0.000 description 1
- 239000011669 selenium Substances 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 229910052717 sulfur Inorganic materials 0.000 description 1
- 239000011593 sulfur Substances 0.000 description 1
- -1 sulfur amino acid Chemical class 0.000 description 1
- 239000003440 toxic substance Substances 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Landscapes
- Fodder In General (AREA)
- Fertilizers (AREA)
Abstract
The invention discloses a compound fermentation inoculant, a method for preparing fermented rapeseed meal for feed and application of the compound fermentation inoculant, and relates to the technical field of biological fermentation. The composite fermentation microbial inoculum comprises rhodotorula rubra, pediococcus acidilactici, aspergillus niger, bacillus coagulans and cellulase produced by the aspergillus niger. The production process for preparing the feed fermented rapeseed meal by using the composite fermentation inoculant provided by the invention is simple to operate, and the prepared fermented rapeseed meal has thick fragrance, wherein the contents of anti-nutritional factors such as glucosinolate, phytic acid, tannin, crude fiber and the like are obviously reduced, the contents of crude protein and acid-soluble protein are obviously increased, the anti-nutritional factors in the rapeseed meal can be effectively removed, and the nutritional quality of the rapeseed meal is improved. The fermented rapeseed meal for feeding is applied to preparing feeds for livestock, poultry and aquatic animals.
Description
Technical Field
The invention relates to the technical field of biological fermentation, in particular to a compound fermentation inoculant, a method for preparing feed fermented rapeseed meal and application thereof, and especially relates to application of the fermented rapeseed meal in replacing soybean meal or fish meal in aquatic feed.
Background
The high-quality protein resources for feeding aquatic animals in China seriously depend on import, and according to incomplete statistics, soybean products are imported in China by more than 5500 ten thousand tons and fish meal is imported by more than 100 ten thousand tons. The lack of good quality feed stocks has become the most serious problem facing the growing aquaculture and aquaculture feed industries. Rape is the first major oil crop in China, rapeseed meal is used as a byproduct after oil pressing of rapeseed, the protein content is rich (35-42 parts), the amino acid composition is reasonable, and the rape contains high sulfur amino acid, lysine, and microelements such as iron, manganese, zinc, selenium and the like. However, because the rapeseed meal contains anti-nutritional factors such as glucosinolate, tannin, phytic acid, isothiocyanate and the like, the rapeseed meal has adverse effects on the growth, development and reproduction of animals, and the great use of the rapeseed meal in animal feed is restricted.
The microbial fermentation method is a method for directly decomposing and converting anti-nutritional factors by means of microbial life metabolic activity, has mild conditions and simple process, can efficiently degrade toxic substances such as glucosinolate, tannin, phytic acid, isothiocyanate and the like, degrade and soften crude fibers, secrete and synthesize a large amount of nutritional and hormone substances such as active probiotics, bioactive small peptide amino acids, biochemical enzymes, growth promoting factors and the like, thereby improving the nutritional value and palatability of the rapeseed meal, increasing the use proportion of the rapeseed meal in the feed and improving the self-supporting rate of high-quality protein sources in China.
Therefore, the method for removing the anti-nutritional factors in the rapeseed meal by using the microbial fermentation method has important significance for improving the self-sufficient rate of high-quality protein sources in China and promoting the sustainable development of aquaculture.
Disclosure of Invention
The invention provides a compound fermentation inoculant, a method for preparing fermented rapeseed meal for feed and application thereof, and aims to solve the problems in the background technology.
In order to achieve the technical purpose, the invention mainly adopts the following technical scheme:
in a first aspect, the invention discloses a composite fermentation inoculant, which comprises rhodotorula rubra, pediococcus acidilactici, aspergillus niger, bacillus coagulans and cellulase produced by the aspergillus niger.
In some embodiments, the complex fermentation inoculant comprises the following components in parts by weight: 1-50 parts of rhodotorula rubra, 1-20 parts of pediococcus acidilactici, 1-15 parts of aspergillus niger, 1-30 parts of bacillus coagulans and 1-10 parts of cellulase.
Preferably, the composite fermentation inoculant comprises the following components in parts by weight: 10-40 parts of rhodotorula rubra, 5-20 parts of pediococcus acidilactici, 1-10 parts of aspergillus niger, 10-25 parts of bacillus coagulans and 5-10 parts of cellulase.
More preferably, the composite fermentation inoculant comprises the following components in parts by weight: 20-30 parts of rhodotorula rubra, 10-20 parts of pediococcus acidilactici, 5-10 parts of aspergillus niger, 20-25 parts of bacillus coagulans and 5-8 parts of cellulase.
In some embodiments, the total number of viable bacteria of the rhodotorula rubra, the pediococcus acidilactici, the aspergillus niger and the bacillus coagulans is 50-100 hundred million CFU/g, 100-300 hundred million CFU/g and 20-50 hundred million CFU/g respectively, and the activity of the cellulase is more than or equal to 2000u/g.
In some embodiments, the rhodotorula rubra, pediococcus acidilactici, aspergillus niger, bacillus coagulans, and cellulase produced from aspergillus niger are from fermented beancurd, kimchi fermented foods.
In a second aspect, the invention discloses an application of the compound fermentation inoculant in preparation of fermented rapeseed meal for feed.
In a third aspect, the invention discloses a method for preparing fermented rapeseed meal for feeding by using the compound fermentation inoculant, which comprises the following steps: the method comprises the steps of crushing rapeseed meal, sieving, uniformly mixing the fermentation inoculum, the rapeseed meal and water according to a certain mass ratio, placing the mixture into a breathing bag, performing fermentation treatment at the temperature of 25-40 ℃, turning over once every 12 hours, fermenting for 1-3 days, drying and crushing after the fermentation is finished, thus obtaining the fermented rapeseed meal for feed.
In some embodiments, the fermentation inoculum, by mass ratio: rapeseed meal: water = (0.1-0.3): 100: (50-120), the fermentation temperature is 30-40 ℃, and the fermentation time is 1-2 days.
Further, the fermentation inoculum comprises the following components in percentage by mass: rapeseed meal: water = (0.1-0.2): 100: (80-100), the fermentation temperature is 30-37 ℃, and the fermentation time is 2 days.
Compared with the prior art, the invention has the following beneficial effects:
the production process for preparing the feed fermented rapeseed meal by using the composite fermentation inoculant provided by the invention is simple to operate, and the prepared fermented rapeseed meal has thick fragrance, wherein the contents of anti-nutritional factors such as glucosinolate, phytic acid, tannin, crude fiber and the like are obviously reduced, the contents of crude protein and acid-soluble protein are obviously increased, the anti-nutritional factors in the rapeseed meal can be effectively removed, and the nutritional value of the rapeseed meal is improved. The fermented rapeseed meal prepared by the invention can replace soybean meal or fish meal in aquatic feed, can obviously reduce feed cost, and improves breeding benefit.
Detailed Description
The technical solution of the present invention will be further described in detail with reference to specific embodiments. It is to be understood that the following examples are only illustrative and explanatory of the present invention and should not be construed as limiting the scope of the present invention. All the technologies realized based on the above-mentioned contents of the present invention are covered in the protection scope of the present invention.
Example 1
And (3) uniformly mixing 20g of rhodotorula rubra, 10g of pediococcus acidilactici, 10g of aspergillus niger, 20g of bacillus coagulans and 8g of cellulase to obtain the fermentation microbial inoculum. Wherein the total number of viable bacteria of rhodotorula rubra, pediococcus acidilactici, aspergillus niger and bacillus coagulans is 50-100 hundred million CFU/g, 100-300 hundred million CFU/g and 20-50 hundred million CFU/g respectively, and the activity of the cellulase is more than or equal to 2000u/g. The zymophyte agents are all from fermented foods such as fermented bean curd, pickle and the like, and the cellulase is produced from aspergillus niger.
0.1g of zymophyte agent is added into 100mL of water, evenly stirred and inoculated into 100g of crushed rapeseed meal, and evenly mixed. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 2 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Example 2
And uniformly mixing 10g of rhodotorula rubra, 20g of pediococcus acidilactici, 8g of aspergillus niger, 25g of bacillus coagulans and 5g of cellulase to obtain the fermentation inoculum. The total number of living bacteria of rhodotorula rubra, pediococcus acidilactici, aspergillus niger and bacillus coagulans is respectively 50-100 hundred million CFU/g, 100-300 hundred million CFU/g and 20-50 hundred million CFU/g, the activity of the cellulase is more than or equal to 2000u/g, the zymophyte agents are all from fermented foods such as fermented bean curd, pickled vegetables and the like, and the cellulase is produced from aspergillus niger.
Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Example 3
And uniformly mixing 30g of rhodotorula rubra, 5g of pediococcus acidilactici, 20g of aspergillus niger, 10g of bacillus coagulans and 10g of cellulase to obtain the fermentation inoculum. The total number of living bacteria of rhodotorula rubra, pediococcus acidilactici, aspergillus niger and bacillus coagulans is respectively 50-100 hundred million CFU/g, 100-300 hundred million CFU/g and 20-50 hundred million CFU/g, the activity of the cellulase is more than or equal to 2000u/g, the zymophyte agents are all from fermented foods such as fermented bean curd, pickled vegetables and the like, and the cellulase is produced from aspergillus niger.
Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 1 day. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Example 4
And (3) uniformly mixing 40g of rhodotorula rubra, 2g of pediococcus acidilactici, 1g of aspergillus niger, 13g of bacillus coagulans and 2g of cellulase to obtain the fermentation microbial inoculum. Wherein the total number of viable bacteria of rhodotorula rubra, pediococcus acidilactici, aspergillus niger and bacillus coagulans is 50-100 hundred million CFU/g, 100-300 hundred million CFU/g and 20-50 hundred million CFU/g respectively, and the activity of the cellulase is more than or equal to 2000u/g. The zymophyte agents are all from fermented foods such as fermented bean curd, pickle and the like, and the cellulase is produced from aspergillus niger.
Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 2 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 1
And (3) taking 20g of rhodotorula rubra and 8g of cellulase, and uniformly mixing to obtain the fermentation inoculant. 0.1g of zymophyte agent is added into 100mL of water, evenly stirred and inoculated into 100g of crushed rapeseed meal, and evenly mixed. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 2
And (3) uniformly mixing 10g of pediococcus acidilactici and 8g of cellulase to obtain the fermentation inoculum. 0.1g of zymophyte agent is added into 100mL of water, evenly stirred and inoculated into 100g of crushed rapeseed meal, and evenly mixed. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 3
And (3) taking 10g of Aspergillus niger and 8g of cellulase, and uniformly mixing to obtain the fermentation inoculum. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 4
And (3) uniformly mixing 20g of bacillus coagulans and 8g of cellulase to obtain the fermentation inoculant. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 5
Taking 50g of rhodotorula rubra, 20g of pediococcus acidilactici, 15g of aspergillus niger, 30g of bacillus coagulans and 10g of cellulase, and uniformly mixing to obtain the fermentation microbial inoculum. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 6
60g of rhodotorula rubra, 30g of pediococcus acidilactici, 20g of aspergillus niger, 40g of bacillus coagulans and 15g of cellulase are uniformly mixed to obtain the fermentation inoculum. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 7
And (3) uniformly mixing 20g of rhodotorula rubra, 10g of lactobacillus, 10g of aspergillus niger, 20g of bacillus coagulans and 8g of cellulase to obtain the fermentation microbial inoculum. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 8
And uniformly mixing 20g of saccharomyces cerevisiae, 10g of pediococcus acidilactici, 10g of aspergillus niger, 20g of bacillus coagulans and 8g of cellulase to obtain the fermentation inoculum. 0.1g of zymophyte agent is added into 100mL of water, evenly stirred and inoculated into 100g of crushed rapeseed meal, and evenly mixed. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 9
And uniformly mixing 20g of rhodotorula rubra, 10g of pediococcus acidilactici, 10g of mucor aculeatus, 20g of bacillus coagulans and 8g of cellulase to obtain the fermentation inoculant. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
Comparative example 10
And (3) uniformly mixing 20g of rhodotorula rubra, 10g of pediococcus acidilactici, 10g of aspergillus niger, 20g of bacillus subtilis and 8g of cellulase to obtain the fermentation microbial inoculum. Adding 0.1g of zymophyte agent into 100mL of water, stirring uniformly, inoculating into 100g of crushed rapeseed meal, and mixing uniformly. Transferring the inoculated rapeseed meal into a breathing bag, sealing and fermenting, controlling the fermentation temperature at 37 ℃, turning over once every 12 hours, and fermenting for 3 days. And after the fermentation is finished, drying at 65 ℃, crushing, and sieving by a 40-mesh sieve to obtain a fermented rapeseed meal finished product.
The results of the examination of the anti-nutritional factors in the fermented rapeseed meal for feed prepared in examples 1 to 4 and comparative examples 1 to 10 are shown in table 1.
TABLE 1 Effect of different fermentation methods on the anti-nutritional factor content of rapeseed meal
As can be seen from Table 1, in the fermented rapeseed meal for feed prepared by using the composite fermentation inoculant provided by the invention, the contents of anti-nutritional factors, namely glucosinolate, tannin and phytic acid, namely crude fiber components are obviously reduced, and are obviously different from those of comparative examples 1-10, wherein P is less than 0.01.
The nutrient contents of the fermented rapeseed meal for feeding prepared in examples 1 to 4 and comparative examples 1 to 10 were measured, and the results are shown in table 2.
TABLE 2 influence of different fermentation methods on the nutrient content of rapeseed meal
As can be seen from Table 2, the feed fermented rapeseed meal prepared by using the composite fermentation inoculant provided by the invention has the advantages that the content of crude protein and acid soluble protein is obviously increased (P is less than 0.05) compared with that of the initial rapeseed meal, and the nutritional value of the feed fermented rapeseed meal prepared by using the composite fermentation inoculant provided by the invention is obviously improved.
The above description is only exemplary of the preferred embodiments of the present invention, and is not intended to limit the present invention, and any modifications, equivalents, improvements, etc. made within the spirit and principle of the present invention should be included in the protection scope of the present invention.
And (3) testing the application effect:
example 5 Effect of application in Tilapia feed
In tilapia feed (crude protein 30%, crude fat 7%), 0%, 25%, 50%, 75% and 100% of soybean meal are replaced by fermented rapeseed meal respectively, and tilapia is fed for 8 weeks, and 4 tilapia groups are parallel. And sampling and analyzing the growth and the feed utilization of the tilapia.
TABLE 3 influence of fermented rapeseed meal instead of soybean meal in different proportions on the growth and feed utilization of tilapia mossambica
| Group of | 0％ | 25％ | 50％ | 75％ | 100％ |
| Initial average body weight/g | 30.20 | 30.51 | 30.26 | 30.55 | 30.42 |
| Average weight at end/g | 197.85 | 202.34 | 199.61 | 191.77 | 175.26 |
| The weight gain rate% | 555.13 | 563.19 | 559.65 | 527.72 | 476.13 |
| Coefficient of feed | 1.32 | 1.35 | 1.34 | 1.38 | 1.43 |
The above results show that the fermented rapeseed meal replaces up to 75% of the soybean meal in the feed for tilapia, without adversely affecting the growth and feed utilization of tilapia.
Example 6 application effects in Micropterus salmoides feed
In the micropterus salmoides feed (53% of crude protein and 10% of crude fat), 0%, 25%, 50%, 75% and 100% of fish meal are respectively replaced by fermented rapeseed meal, and micropterus salmoides are fed for 8 weeks, and 4 pieces of micropterus salmoides are fed in parallel. And (5) sampling and analyzing the growth and the feed utilization of the largemouth bass.
TABLE 4 influence of fermented rapeseed meal instead of fish meal in different proportions on growth and feed utilization of Lateolabrax
| Group of | 0％ | 25％ | 50％ | 75％ | 100％ |
| Initial average body weight/g | 5.22 | 5.30 | 5.25 | 5.15 | 5.30 |
| Average weight at end/g | 25.26 | 24.65 | 20.71 | 15.83 | 10.83 |
| The weight gain rate% | 383.91 | 365.09 | 302.14 | 198.68 | 104.34 |
| Coefficient of feed | 0.75 | 0.78 | 0.84 | 0.96 | 1.22 |
The results show that the fermented rapeseed meal can replace 25% of fish meal in the feed of the micropterus salmoides at most without obviously reducing the growth performance and the feed utilization of the micropterus salmoides.
Claims (10)
1. A compound fermentation inoculant is characterized in that: including Rhodotorula rubra, pediococcus acidilactici, aspergillus niger, bacillus coagulans, and cellulase produced by Aspergillus niger.
2. The complex fermentation inoculant according to claim 1, wherein: the composite fermentation inoculant comprises the following components in parts by weight: 1-50 parts of rhodotorula rubra, 1-20 parts of pediococcus acidilactici, 1-15 parts of aspergillus niger, 1-30 parts of bacillus coagulans and 1-10 parts of cellulase.
3. The complex fermentation inoculant according to claim 2, wherein: the composite fermentation inoculant comprises the following components in parts by weight: 10-40 parts of rhodotorula rubra, 5-20 parts of pediococcus acidilactici, 1-10 parts of aspergillus niger, 10-25 parts of bacillus coagulans and 5-10 parts of cellulase.
4. The complex fermentation inoculant according to claim 3, wherein: the composite fermentation inoculant comprises the following components in parts by weight: 20-30 parts of rhodotorula rubra, 10-20 parts of pediococcus acidilactici, 5-10 parts of aspergillus niger, 20-25 parts of bacillus coagulans and 5-8 parts of cellulase.
5. The complex fermentation inoculant according to any one of claims 1 to 4, wherein: the total number of viable bacteria of the rhodotorula rubra, the pediococcus acidilactici, the aspergillus niger and the bacillus coagulans is 50-100 hundred million CFU/g, 100-300 hundred million CFU/g and 20-50 hundred million CFU/g respectively, and the activity of the cellulase is more than or equal to 2000u/g.
6. The complex fermentation inoculant according to claim 5, wherein: the rhodotorula rubra, pediococcus acidilactici, aspergillus niger, bacillus coagulans and cellulase produced by aspergillus niger are from fermented bean curd and pickle fermented food.
7. The use of the complex fermentation inoculant according to claim 5 in the preparation of fermented rapeseed meal for feed.
8. A method for preparing fermented rapeseed meal for feed by using the compound fermentation inoculant as defined in claim 5, which is characterized by comprising the following steps: the method comprises the steps of crushing rapeseed meal, sieving, uniformly mixing a fermentation microbial inoculum, the rapeseed meal and water according to a certain mass ratio, placing the mixture in a breathing bag, performing fermentation treatment at the temperature of 25-40 ℃, turning over once every 12 hours, fermenting for 1-3 days, drying and crushing after fermentation is completed, thus obtaining the fermented rapeseed meal for feeding.
9. The method of claim 8, wherein: the fermentation inoculum comprises the following components in percentage by mass: rapeseed meal: water = (0.1-0.3): 100: (50-120), the fermentation temperature is 30-40 ℃, and the fermentation time is 1-2 days.
10. The method of claim 9, wherein: the fermentation inoculum comprises the following components in percentage by mass: rapeseed meal: water = (0.1-0.2): 100: (80-100), the fermentation temperature is 30-37 ℃, and the fermentation time is 2 days.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211608483.4A CN115851465B (en) | 2022-12-14 | 2022-12-14 | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feeding and application of composite fermentation inoculant |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN202211608483.4A CN115851465B (en) | 2022-12-14 | 2022-12-14 | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feeding and application of composite fermentation inoculant |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN115851465A true CN115851465A (en) | 2023-03-28 |
| CN115851465B CN115851465B (en) | 2024-01-30 |
Family
ID=85672956
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN202211608483.4A Active CN115851465B (en) | 2022-12-14 | 2022-12-14 | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feeding and application of composite fermentation inoculant |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN115851465B (en) |
Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703968A (en) * | 2004-05-25 | 2005-12-07 | 中国农业大学 | Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage |
| CN101897383A (en) * | 2010-06-29 | 2010-12-01 | 北京金泰得生物科技股份有限公司 | Method for removing cotton rapeseed meal toxicant by fermentation method and enhancing nutrient value thereof, feeding fermented cotton rapeseed meal protein feedstock and applications thereof |
| KR20120022291A (en) * | 2010-09-01 | 2012-03-12 | 한국과학기술원 | A livestock feed additive comprising the fermented biotite using effective microorganisms for enhancing viability of livestock |
| CN105494890A (en) * | 2014-09-26 | 2016-04-20 | 侯文燕 | Enzymolysis and fermentation combined method for improving feeding nutritional value of rapeseed meal |
| CN106399189A (en) * | 2016-10-29 | 2017-02-15 | 湖北省生物农药工程研究中心 | Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces |
| CN106689692A (en) * | 2016-11-25 | 2017-05-24 | 彭程 | Protein feed for substituting soybean meal or fish meal |
| CN111296723A (en) * | 2020-03-02 | 2020-06-19 | 淮阴工学院 | Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof |
| CN113073056A (en) * | 2020-01-06 | 2021-07-06 | 安琪酵母股份有限公司 | Fermentation inoculant for rapeseed meal and application thereof |
| CN113229399A (en) * | 2021-04-28 | 2021-08-10 | 四川生力源生物工程有限公司 | Method for biologically degrading rapeseed meal toxin and improving nutritive value thereof |
-
2022
- 2022-12-14 CN CN202211608483.4A patent/CN115851465B/en active Active
Patent Citations (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1703968A (en) * | 2004-05-25 | 2005-12-07 | 中国农业大学 | Synbiotic feed addictive of mini-peptide with beneficial microbe and its usage |
| CN101897383A (en) * | 2010-06-29 | 2010-12-01 | 北京金泰得生物科技股份有限公司 | Method for removing cotton rapeseed meal toxicant by fermentation method and enhancing nutrient value thereof, feeding fermented cotton rapeseed meal protein feedstock and applications thereof |
| KR20120022291A (en) * | 2010-09-01 | 2012-03-12 | 한국과학기술원 | A livestock feed additive comprising the fermented biotite using effective microorganisms for enhancing viability of livestock |
| CN105494890A (en) * | 2014-09-26 | 2016-04-20 | 侯文燕 | Enzymolysis and fermentation combined method for improving feeding nutritional value of rapeseed meal |
| CN106399189A (en) * | 2016-10-29 | 2017-02-15 | 湖北省生物农药工程研究中心 | Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces |
| CN106689692A (en) * | 2016-11-25 | 2017-05-24 | 彭程 | Protein feed for substituting soybean meal or fish meal |
| CN113073056A (en) * | 2020-01-06 | 2021-07-06 | 安琪酵母股份有限公司 | Fermentation inoculant for rapeseed meal and application thereof |
| CN111296723A (en) * | 2020-03-02 | 2020-06-19 | 淮阴工学院 | Probiotic agent for multi-strain combined solid state fermentation of rapeseed meal and fermentation method thereof |
| CN113229399A (en) * | 2021-04-28 | 2021-08-10 | 四川生力源生物工程有限公司 | Method for biologically degrading rapeseed meal toxin and improving nutritive value thereof |
Non-Patent Citations (3)
| Title |
|---|
| 管维;兰时乐;詹逸舒;李倩;谭日晓;王璇;: "复合微生物固态发酵菜籽饼粕对抗营养因子去除条件的研究", 安徽农业科学, no. 02, pages 197 - 199 * |
| 陈君 等: "多菌种混合发酵对菜籽饼脱毒的研究", 安徽农业科学, vol. 39, no. 36 * |
| 韩财安 等: "深红酵母菌在台湾泥鳅养殖中的应用", 现代农业研究, vol. 26, pages 73 - 74 * |
Also Published As
| Publication number | Publication date |
|---|---|
| CN115851465B (en) | 2024-01-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| KR100840145B1 (en) | Fermented feeds for livestock farming using lactic acid bacteria and yeast and processing method thereof | |
| CN101779749B (en) | Production method for adding brewer grain fattening pig feed | |
| CN105614023B (en) | Fermentation enzymolysis agent for soybean meal fermentation and application thereof | |
| CN110679728A (en) | Preparation method and application of fermented rice bran feed | |
| CN109287865B (en) | Compound fermented protein feed and preparation method thereof | |
| CN103070286B (en) | Preparation method of micro-storage straw feed | |
| CN108142728A (en) | Fish fermented feed and its preparation method and application | |
| CN110583866A (en) | High-dietary-fiber low-antigen-protein fermented soybean hull and preparation method thereof | |
| CN101194668B (en) | Process for preparing blood meal biological modified peptide protein and application of the same | |
| CN111642640A (en) | Broussonetia papyrifera feed vegetable protein starter culture for pigs and preparation method thereof | |
| CN111743043A (en) | Additive feed for fattening beef cattle and use method thereof | |
| CN106035990B (en) | Method for preparing biological feed by solid-state enzymolysis fermentation of citrus pulp, product and application thereof | |
| CN113073056A (en) | Fermentation inoculant for rapeseed meal and application thereof | |
| CN115152892B (en) | Method for degrading free gossypol in cottonseed meal, product and application thereof | |
| CN110558420A (en) | High-dietary-fiber low-antigen-protein fermented soybean hull | |
| CN116391796A (en) | Method for processing wangcao | |
| CN111248354A (en) | Sheep feed based on fermented bamboo shoot shells and preparation method thereof | |
| CN116195679A (en) | Method for improving quality of rapeseed meal through synergistic fermentation of bacterial enzymes | |
| CN110477190A (en) | A kind of stalk anaerobic fermentation feed and preparation process and formula forage | |
| CN102813055A (en) | EM (effective microorganisms) fermented straw high-protein mixed feed and production method thereof | |
| CN115851465B (en) | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feeding and application of composite fermentation inoculant | |
| CN105639115B (en) | Amino acid-enhanced fermented and enzymolyzed soybean meal and application thereof | |
| CN111213793A (en) | Compound fermented biological feed and preparation method thereof | |
| CN100341421C (en) | Method for producing biological protein drink by king grass | |
| CN115316570B (en) | Biological feed for culturing hermetia illucens and preparation method thereof |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| SE01 | Entry into force of request for substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| GR01 | Patent grant | ||
| GR01 | Patent grant |