CN106399189A - Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces - Google Patents
Streptomyces having inhibiting effect on Corynespora cassiicola and application of Streptomyces Download PDFInfo
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- CN106399189A CN106399189A CN201610923326.0A CN201610923326A CN106399189A CN 106399189 A CN106399189 A CN 106399189A CN 201610923326 A CN201610923326 A CN 201610923326A CN 106399189 A CN106399189 A CN 106399189A
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
- C12N1/205—Bacterial isolates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
- C12R2001/00—Microorganisms ; Processes using microorganisms
- C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
- C12R2001/465—Streptomyces
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/20—Bacteria; Culture media therefor
Abstract
The invention discloses Streptomyces having an inhibiting effect on Corynespora cassiicola and application of the Streptomyces. The Streptomyces is Streptomyces phaeoluteichromatogenes HEBRC-45958, and the preservation number of the Streptomyces phaeoluteichromatogenes is CCTCC No. M2015768. The Streptomyces has the advantages that the Streptomyces phaeoluteichromatogenes can generate fermentation broth with high antibacterial activity after fermentation, and the fermentation broth is good in preventing and treating effect on Corynespora leaf spot caused by the Corynespora cassiicola; compared with current chemically-synthesized bactericide, the Streptomyces phaeoluteichromatogenes comes from the natural environment and is efficient, low in toxicity, free of environment pollution, less prone to tolerance generating, and the like.
Description
Technical field
The invention belongs to crops Prevention Technique field is and in particular to one plant possesses the chain of inhibitory action to how main rod spore bacterium
Mould and its application.
Background technology
How main rod spore bacterium (Corynespora cassiicola), is under the jurisdiction of Deuteromycotina, Hyphomycetes, hyphomycetales, dark
Color Cordycepps, Corynespora, are a kind of host range widely universal plant pathogenic fungis, be find in this genus earliest,
Host range kind the widest.Can infect the torrid zone and subtropical zone nearly 380 belong in 530 kinds of plants, including cucumber, tomato,
Cotton, cowpea, soybean, pawpaw, tobacco and rubber etc. (Smith, 2008).The blade of this germ main harm plant, forms allusion quotation
Type has the necrotic plaque of yellow halo, also can infect root, stem, flower and fruit, cause the phenomenons such as fallen leaves, shedding when serious.
1958 in India's reported first by how main rod spore microbial rubber tree rod spore mould defoliation
(Ramakrishnan an d Pilla, 1961), this disease of 50-80 age in 20th century is on the main product glue ground in Asia-Pacific and Africa
Area occurs in succession, but because this disease is only limitted on nursery and immature plant and causes harm less, always as one
Plant Minor diseases and do not cause the concern of people.But disease survey finds in recent years, by the how main rod microbial phytopathy of spore
Evil produces to cause to multiclass industrial crops in the world and has a strong impact on, and causes huge economic losses, such as in South American region
How main rod spore bacterium causes the excellent spore tikka of lipstick flower, red sage, poinsettia, water Chinese littleleaf box and the garden crop such as cucumber, tomato, capsicum
Disease；In Middle West area, mainly cause the excellent spore leaf spot of the crops such as soybean, cowpea and sesame, the loss of tomato planting ground
Reach 11800 kg/ha (Pernez ny et al., 1996) it is estimated that, once what the serious mould defoliation of excellent spore occurred is
Evil, rubber tree production loss nearly 20%；In addition, in the Asia such as Brazil, Philippine, Nigeria, India and Korea S and Japan
Also there is the report (Chee, 1990) of excellent spore leaf spot in continent country.
In China, Wei Jingchao finds cowpea rod spore leaf spot early than nineteen fifty in lower course area of Yellow River, but due to cowpea
Cultivated area is limited, does not draw attention.Early 1990s, the purpleflower violet herb such as China Henan, Liaoning, eggplant vegetable growing ground
It is subject to generally infecting of how main rod spore, cause certain economic loss.The investigation of 1994 finds, cucumber rod spore leaf spot exists
The ground such as ShenYang, Liaoning Province, Wafangdian and Dandong generally occur, but do not extend and spread.But in recent years, the harm of this disease is in significantly
Ascendant trend, 2005 so far, and this disease is in 19 provinces, cities and regions such as Shandong, Hebei, Beijing, Liaoning, Jilin, Henan, Yunnan, Hainan
Break out greatly, the vegetables such as main harm cucumber, tomato, eggplant, Kidney bean, more than 10,000,000 mu, loss is more than 50 for annual occurring area
Hundred million.And the most serious with dependent territory cucumber harm, the typically disease field leaf incidence of disease is 10%-25%, up to 60%- when serious
70%, or even 100%, because excellent spore leaf spot endangers seriously in China vegetables and rubber planting area, and have resulted in great
Economic loss, seriously constrains the development of China vegetables and Rubber Industry.If this disease can not be controlled effectively, will be to me
The important vegetables such as state cucumber, tomato and eggplant and Rubber Industry cause destructive strike.
At present, chemical prevention remains the Main Means preventing and treating this disease, it has, and range is wide, instant effect, use
Convenient, can timely and effectively control as crop rod this paroxysmal disease of spore leaf spot.Abroad previously carry out preventing and treating
The plant selection and application of rubber bar spore leaf spot bactericide is it is recommended that preferable medicament has carbendazim, thiophanate-methyl and Dai Sen
MnZn.Methoxy acrylic bactericide has been applied to the preventing and treating of vegetables rod spore leaf spot in Japan, and day in 2006 is originally
Boscalid is registered as preventing and treating the medicament of cucumber rod spore leaf spot, and achieves good prevention effect (Miyamoto et
Al., 2009).
Although chemical agent plays an important role in the preventing and treating of crop rod spore leaf spot, due to it frequently
Use, also create drug-fast problem, early in 1979, western Japan great Fen county cucumber rod spore leaf spot had become as locality
The important disease of cucumber production, initially use benzimidazole germicide carries out preventing and treating and achieves good effect, but due to
Drug-fast generation preventive effect begins to decline (Hasama, 1991).In July, 2006, Boscalid is in Japan as Japanese anti-Yellow River harnessing
The registration medicament of melon rod spore leaf spot, is subsequently monitored to its sensitiveness, in the bacterial strain to 438 cucumber rod spore leaf spots
Find in drug-fast detection, 214 plants are developed immunity to drugs to Boscalid, fastness frequency is up to 49% (MIC>30 μ g/ml), its
In 189 bacterial strains be middle antibacterial strain (EC501.1-6.3 μ g/ml), 25 bacterial strains are superelevation antibacterial strain (EC50>24.8μg/ml)(M
Iyamoto et al, 2009).
For China's crop rod spore leaf spot, Antitubulin carbendazim is also had with the diethofencarb resistance to the action of a drug and mutually put into
System research.Research shows China how main rod spore bacterium to carbendazim resistance very high frequency, and dual anti-to carbendazim and diethofencarb
Frequency is relatively low.To picking up from 12 areas of China, the main rod sensitiveness to carbendazim and diethofencarb for the spore bacterium more than 163 plants of 18 hosts
It is analyzed, 145 bacterial strains have the resistance to the action of a drug to carbendazim, and fastness frequency is up to 89.0%.Only 18 bacterial strains are quick to carbendazim
Sense, accounts for the 11.0% of strains tested.24 bacterial strains have the dual resistance to the action of a drug to carbendazim and diethofencarb, and dual anti-frequency is 14.7%
(Huang et al., 2013).Understand from research, although carbendazim does not have the registration medicament as excellent spore leaf spot, real
The very serious resistance to the action of a drug has been produced on border.
Actinomyces (Actinomycetes) are a kind of very important microbe groups of nature, belong to procaryotic
One monoid.Actinomycetal (Actinomyeetales).It is very abundant in nature, has in terms of control of crop disease
There are huge use value and economic worth, the metabolite (especially antibiotic) that it produces plays in control of crop disease
Significant role, antibiotic have efficiently, low toxicity, degradable, noresidue, environmentally friendly the features such as, this is that chemical pesticide does not have
Have, in recent years, all constantly find new Antibiotics, such as jinggangmeisu, kasugarnycin, Ningnanmycin, Wuyi both at home and abroad
Mycin and Zhongshengmycin etc..But the actinomyces being separated at present are only the 0.1-1% of actual species, therefore it has very big answering
With and development space, there is presently no the report of the excellent spore leaf spot of antibiotic preventing and treating of actinomyces active bacteria formulation and its generation.Open
The new type bactericide sending preventing and treating crop rod spore leaf spot has great practice significance.
Content of the invention
Possesses the streptomycete of inhibitory action it is an object of the invention to provide a kind of to how main rod spore bacterium, described streptomycete
For producing isabelline streptomycete (Streptomyces phaeoluteichromatogenes) HEBRC-45958, deposit number is：
CCTCC NO：M2015768.This bacterial strain possesses inhibitory action to how main rod spore bacterium, can be used for the preventing and treating of plant rod spore leaf spot.
Further object is that providing the preparation method of streptomycete HEBRC-45958, method is simple, easily
OK, the zymotic fluid being obtained using the method, active principle content is high, possesses good prospect of production.
Final object of the present invention there are provided streptomycete HEBRC-45958 in preparation preventing and treating plant rod spore tikka
Application in medicine.
In order to achieve the above object, the present invention takes technical scheme below to realize.
One plant has good inhibitory action streptomycete to how main rod spore bacterium, grinds the soil of mountain collection from Wuhan East Lake and divides
From, belong to the isabelline streptomycete of product (Streptomyces phaeoluteichromatogenes) after 16s rDNA identification,
Deliver to China typical culture collection center on December 23rd, 2015 and carry out preservation, Classification And Nomenclature：Produce isabelline streptomycete
(Strept omyces phaeoluteichromatogenes)HEBRC-45958；Deposit number is CCTCC NO：
M2015768；Place：China, Wuhan, Wuhan University.
Produce isabelline streptomycete (Streptomyces phaeoluteichromatogenes) HEBRC-45958 in ISP-
Well-grown on 2 culture mediums, bacterium colony is isabelline, and spore surface is smooth.
Produce the preparation method of isabelline streptomycete HEBRC-45958, including：
Proceed to fermentation medium by producing isabelline streptomycete HEBRC-45958 seed liquor by the inoculum concentration of 3-10% (V/V)
In.Temperature is 28 DEG C, mixing speed 200-500 rev/min of fermentation tank, and the loading amount of fermentation medium is the 60%- of tank volume
80%；Fermentation 4-6 days；
The formula of described fermentation medium includes：Mannitol 1-3%, soy peptone 1-3%, dusty yeast 0.3-
0.5%th, calcium carbonate 0.3-0.5%, sodium chloride 0.3-0.8%；PH value 6.5-7.5, remaining is water, and above is all mass ratio.
Preferably, the preparation method of described streptomycete HEBRC-45958 seed liquor includes:
Inclined-plane is preserved bacterial classification activate in ISP-2 flat board, picking is seeded in 100 milliliters of seed culture mediums, at 28 DEG C,
Cultivate 3-4 days on 130-160 rev/min of shaking table；Both obtained seed liquor.
The formula of described seed culture medium is identical with fermentation medium.
Produce isabelline streptomycete HEBRC-45958 preparation preventing and treating plant rod spore tikka medicine in application, including by
The zymotic fluid of this bacterial strain or zymotic fluid dilution after be directly sprayed on plant leaf portion and/or stem, or with other active ingredients
Make compound formulation in case controlling plurality of plant diseases it is preferred that zymotic fluid and water are by 1：20-1:100 (volume ratio) dilutes.
As long as the isabelline streptomycete of product being provided by all available present invention of how main rod spore microbial rod spore leaf spot
The preparation of HEBRC-45958 preparation is prevented and treated.
Described plant includes but is not limited to cucumber, tomato, eggplant and rubber tree.
Compared with prior art, the present invention has advantages below：
(1) reported first streptomycete is applied to prevent and treat plant rod spore leaf spot, its anti-medicine with existing bactericide no interactions
Property.
(2) compared with using chemical synthesis bactericidal agent for preventing and treating at present, because it comes from natural environment, have efficient, low
Malicious, free from environmental pollution and the advantages of be not likely to produce the resistance to the action of a drug.
Brief description
Fig. 1 is spore on ISP-2 culture medium for the HEBRC-45958 bacterial strain and colonial morphology.
Left figure is that bacterial strain HEBRC-45958 microscope (1000X) observes photo, and right figure is the flat of bacterial strain HEBRC-45958
Plate colonial morphology.
Fig. 2 is HEBRC-45958 and how main rod spore bacterium (C.cassiicola) opposite culture.
Fig. 3 is comparison and process hypha form under microscope.
Fig. 4 is HEBRC-45958 zymotic fluid to tomato rod spore leaf spot prevention effect.
Fig. 5 is HEBRC-45958 zymotic fluid to eggplant rod spore leaf spot prevention effect.
Specific embodiment
In order to preferably explain the present invention, it is further elucidated with the main contents of the present invention below in conjunction with specific embodiment, but
Present disclosure is not limited solely to following examples.Technical scheme of the present invention, if not otherwise specified, is this area
Routine techniques, described reagent or material, if not otherwise specified, derive from commercial channel.
Embodiment 1：
One plant has good inhibitory action streptomycete to how main rod spore bacterium, grinds the soil of mountain collection from Wuhan East Lake and divides
From, belong to the isabelline streptomycete of product (Streptomyces phaeoluteichromatogenes) after 16s rDNA identification,
Deliver to China typical culture collection center on December 23rd, 2015 and carry out preservation, Classification And Nomenclature：Produce isabelline streptomycete
(Streptomy ces phaeoluteichromatogenes)HEBRC-45958；Deposit number is CCTCC NO：
M2015768；Place：China, Wuhan, Wuhan University.In the present invention, produce isabelline streptomycete (Streptomyces
Phaeoluteichromatoge nes) HEBRC-45958 or be referred to as HEBRC-45958.
Morphological feature：Well-grown on ISP-2 culture medium, bacterium colony is isabelline, spore surface smooth (Fig. 1).
Bacterial strain physio-biochemical characteristics：
Table 1 HEBRC-45958 bacterial strain utilization of carbon source
+：The positive ,-：Negative
Embodiment 2：
Produce the preparation method of isabelline streptomycete HEBRC-45958, including：
Inclined-plane is preserved bacterial classification activate in ISP-2 flat board, picking is seeded in 100 milliliters of seed culture mediums, at 28 DEG C,
Cultivate 4 days on 160 revs/min of shaking table；Both obtained seed liquor.
The formula of described seed culture medium is identical with fermentation medium.
Streptomycete HEBRC-45958 seed liquor is proceeded in fermentation medium by the inoculum concentration of 5% (V/V).Temperature is 28
DEG C, 300 revs/min of the mixing speed of fermentation tank, the loading amount of fermentation medium is the 80% of 20L tank volume；Fermentation 6 days；
Prepared zymotic fluid is used for embodiment 4.
The formula of described fermentation medium includes：：Mannitol 2%, soy peptone 1.5%, dusty yeast 0.3%, carbon
Sour calcium 0.5%, sodium chloride 0.5%；PH value 7, is more than mass ratio, and remaining is water.
Embodiment 3：
Produce isabelline streptomycete (S.phaeoluteichromatogenes) HEBRC-45958 to how main rod spore bacterium
(C.cassii cola) in vitro inhibition：
Inclined-plane is preserved HEBRC-45958 bacterial strain activate in ISP-2 flat board, 28 DEG C of culture 4d, beaten with card punch and take 4mm big
Little bacterium dish, is positioned over PDA plate central authorities.Inclined-plane is preserved how main rod spore bacterium (C.cassiicola) and activates 5d in PDA culture medium,
Beat and take 4mm bacterium dish placing four at HEBRC-45958 bacterium dish surrounding 25mm, after opposite culture 7d, observe antibacterial band.Picking
How main rod spore bacterium (C.cassiicola) and HEBRC-45958 face-off edge mycelia, process under the microscope and compare mycelia shape
State difference.
HEBRC-45958 produces significantly antibacterial band, antibacterial bandwidth with how main rod spore bacterium (C.cassiicola) face-off
25mm (Fig. 2), has strong inhibitory action, and compares Hyphal form ratio, processes mycelia and there occurs that distortion, swelling and deformation (are schemed
3).
Embodiment 4：
Produce isabelline streptomycete (S.phaeoluteichromatogenes) HEBRC-45958 preventing and treating crop rod spore tikka
Sick effect：
By tomato and eggplant seed vernalization, it is seeded in 32 holes and 72 hole disks respectively, greenhouse Routine Management, after growth 20d
For pot experiment.For examination pathogenicbacteria separation from field occur excellent spore leaf spot tomato and eggplant, and protect under the conditions of 4 DEG C
It is stored in PDA inclined-plane.Activated, expanded numerous, 28 DEG C of illumination cultivation 12d in PDA culture medium, added sterilized water brush swipe spore
Son, using filtered through gauze.Blood counting chamber observes spore count, using sterilized water, spore concentration is adjusted to 1 × 105/ ml, adds
Final concentration of 0.1% Tween 80 is as dispersant.
HEBRC-45958 zymotic fluid prepared by embodiment 2 dilutes 50 times, is sprayed on tomato and eggplant leaf portion to dripping,
Tomato and eggplant rod spore leaf spot fungi spore suspension is sprayed to blade face saturation after 24h.Dissipated with spraying 50% Boscalid moisture
As comparison medicament, active ingredient concentration is 333.3 μ g/ml to granula.As blank, each processes three weights to spray clear water
Multiple.In 28 DEG C of moisturizing cultures of growth cabinet.Statistics comparison and each process disease index after 4d, and calculate preventive effect.Tomato and eggplant
Son rod spore leaf spot disease index grade scale is following (Ishii et al., 2007).
0 grade：Disease-free symptom
0.1 grade：Scab is less than leaf area 1%
0.5 grade：Scab accounts between leaf area 1%-5%
1 grade：Scab accounts between leaf area 6%-10%
2 grades：Scab accounts between leaf area 11%-20%
3 grades：Scab accounts between leaf area 21%-30%
4 grades：Scab accounts between leaf area 31%-40%
5 grades：Scab accounts between leaf area 41%-50%
6 grades：Scab accounts between leaf area 51%-60%
7 grades：Scab accounts between leaf area 61%-70%
8 grades：Scab accounts between leaf area 71%-80%
9 grades：Scab accounts between leaf area 81%-90%
10 grades：Scab is more than leaf area more than 90%
According to table 2 and 50 times of 3, HEBRC-45958 zymotic fluid dilution, tomato and eggplant rod spore leaf spot are had good
Good prevention effect (Fig. 4,5), preventive effect respectively reaches 88.53% and 91.64%, and compares chemical agent Boscalid preventive effect phase
When.
Table 2 HEBRC-45958 is to tomato rod spore leaf spot prevention effect
| Process | Disease index | Preventive effect (%) |
| HEBRC-45958 | 11.11 | 88.53 |
| Boscalid | 12.34 | 87.26 |
| Clear water compares | 96.88 | - |
Table 3 HEBRC-45958 is to eggplant rod spore leaf spot prevention effect
| Process | Disease index | Preventive effect (%) |
| HEBRC-45958 | 7.33 | 91.64 |
| Boscalid | 9.41 | 89.27 |
| Clear water compares | 87.67 | - |
Claims (5)
1. a kind of detached streptomycete, described streptomycete is to produce isabelline streptomycete（Streptomyces
phaeoluteichromatogenes）HEBRC-45958, its culture presevation number is CCTCC NO： M2015768.
2. application in the excellent spore tikka medicine of preparation preventing and treating for the streptomycete described in claim 1.
3. application in the medicine of preparation suppression how main rod spore bacteria growing for the streptomycete described in claim 1.
4. the zymotic fluid of the streptomycete preparation described in claim 1.
5. the preparation method of zymotic fluid described in claim 4, including：
The seed liquor of streptomycete described in claim 1 is proceeded in fermentation medium by the inoculum concentration of 3-10%V/V;Temperature is 28
DEG C, mixing speed 200-500 rev/min of fermentation tank, the loading amount of fermentation medium is the 60%-80% of tank volume；Fermentation 4-
6 days；
The formula of described fermentation medium includes：Mannitol 1-3%, soy peptone 1-3%, dusty yeast 0.3-0.5%, carbon
Sour calcium 0.3-0.5%, sodium chloride 0.3-0.8%；PH value 6.5-7.5, remaining is water, and above is all mass ratio.
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851465A (en) * | 2022-12-14 | 2023-03-28 | 湖北省生物农药工程研究中心 | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feed and application of composite fermentation inoculant |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6558940B2 (en) * | 2000-03-30 | 2003-05-06 | Council Of Scientific And Industrial Research | Streptomyces strain with potential anti-microbial activity against phytopathogenic fungi |
| CN103114064A (en) * | 2013-03-08 | 2013-05-22 | 浙江省柑桔研究所 | Marine actinomycete with antibacterial activity to multiple plant pathogens |
| CN103740606A (en) * | 2013-11-08 | 2014-04-23 | 湖北省生物农药工程研究中心 | Streptomyces phytohabitans, method for producing new antibiotics Novonestmycin from Streptomyces phytohabitans, and application of Novonestmycin |
-
2016
- 2016-10-29 CN CN201610923326.0A patent/CN106399189B/en active Active
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6558940B2 (en) * | 2000-03-30 | 2003-05-06 | Council Of Scientific And Industrial Research | Streptomyces strain with potential anti-microbial activity against phytopathogenic fungi |
| CN103114064A (en) * | 2013-03-08 | 2013-05-22 | 浙江省柑桔研究所 | Marine actinomycete with antibacterial activity to multiple plant pathogens |
| CN103740606A (en) * | 2013-11-08 | 2014-04-23 | 湖北省生物农药工程研究中心 | Streptomyces phytohabitans, method for producing new antibiotics Novonestmycin from Streptomyces phytohabitans, and application of Novonestmycin |
Non-Patent Citations (2)
| Title |
|---|
| HOOI-LENG SER等: "Streptomyces malaysiense sp. nov.: A novel Malaysian mangrove soil actinobacterium with antioxidative activity and cytotoxic potential against human cancer cell lines", 《SCIENTIFIC REPORTS》 * |
| ZHONGYI WAN等: "Novonestmycins A and B, two new 32-membered bioactive macrolides from Streptomyces phytohabitans HBERC-20821", 《THE JOURNAL OF ANTIBIOTICS》 * |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN115851465A (en) * | 2022-12-14 | 2023-03-28 | 湖北省生物农药工程研究中心 | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feed and application of composite fermentation inoculant |
| CN115851465B (en) * | 2022-12-14 | 2024-01-30 | 湖北省生物农药工程研究中心 | Composite fermentation inoculant, method for preparing fermented rapeseed meal for feeding and application of composite fermentation inoculant |
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