CN115836677A - Seed stem water-retaining agent for cassava and use method - Google Patents
Seed stem water-retaining agent for cassava and use method Download PDFInfo
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Abstract
A seed stem water-retaining agent for cassava belongs to the technical field of agricultural production, and comprises the following components in parts by weight: 0.1 to 10.0 parts of polyglutamic acid, 0.1 to 10.0 parts of chitosan, 1.0 to 10.0 parts of seaweed extract, 5.0 to 20.0 parts of insect repellent substance, 0.1 to 10.0 parts of amino acid aqueous solution and 30.0 to 60.0 parts of film-forming agent aqueous solution. The water-retaining functional substance, the insect repellent substance and the film-forming agent in the cassava seed stem water-retaining agent have the combined action of reducing the water loss of cassava seed stems in the storage process, reducing the occurrence of diseases and insect pests and enhancing the activity of the seed stems, so that the survival rate of the planted seedlings of cassava is improved.
Description
Technical Field
The invention relates to the technical field of agricultural production, and particularly provides a cassava seed stem water-retaining agent and a using method thereof.
Background
Cassava (Manihot esculenta Crantz) is the second potato crop in the world, has root tubers rich in starch, has the reputation of starch king and is one of industrial starch raw materials. Cassava can be planted in dry land in hills with relatively poor soil as drought-tolerant crops, but drought still becomes a factor for restricting the yield of the cassava, so that the improvement of the drought resistance of the cassava is an effective way for improving the yield of the cassava in the dry land. At present, drought-resistant cultivation measures mainly comprise film covering and soil water-retaining agent application, and although the measures can solve the problem of growth and development of cassava after seedling establishment in dry land, the measures rarely relate to the aspect of how to maintain the stem moisture and the activity of cassava seeds before planting.
The cassava is bred in a seed stem planting mode, the harvested cassava seed stems are usually stored for 2 to 3 months, even 4 to 5 months, and the cassava seed stems are not only subjected to large water loss during storage, but also subjected to seed stem loss such as mildew or insect gnawing, and the like, so that the quality of the seed potatoes is seriously influenced, and therefore, the reduction of the water loss during the cassava storage and the improvement of the seed stem storage quality are important factors for improving the survival rate of cassava seedlings subjected to drought.
Disclosure of Invention
The technical problem to be solved by the invention is to overcome the defects of the prior art, and provide the seed stem water-retaining agent for cassava, wherein the water-retaining functional substance, the insect-repellent substance and the film-forming agent in the seed stem water-retaining agent act together, so that the water loss of the cassava seed stems in the storage process is reduced, the occurrence of diseases and insect pests is reduced, and the activity of the seed stems is enhanced, thereby improving the survival rate of the cassava seedlings planted.
The technical problem of the invention is realized by the following technical scheme:
the seed stem water-retaining agent for cassava comprises the following components in parts by weight: 0.1 to 10.0 parts of polyglutamic acid, 0.1 to 10.0 parts of chitosan, 1.0 to 10.0 parts of seaweed extract, 5.0 to 20.0 parts of insect repellent substance, 0.1 to 10.0 parts of amino acid aqueous solution and 30.0 to 60.0 parts of film-forming agent aqueous solution.
The seed stem water-retaining agent is prepared by soaking cassava plant in an insect repellent substance, wherein the cassava plant comprises leaves, stems or root barks.
The film-forming agent is acrylic acid or 108 glue, and the film-forming agent is diluted by water to obtain a film-forming agent water solution.
The seed stem water-retaining agent is prepared by crushing fresh cassava leaves, stems and root barks, and quickly putting the crushed plant fragments into warm water for fully stirring; and (4) leaching the cassava extract after suction filtration to obtain the insect repellent substance.
In the seed stem water-retaining agent, the film-forming agent aqueous solution is an acrylic acid aqueous solution with the mass concentration of 0.5% or a 108 glue aqueous solution with the mass concentration of 50%, and the amino acid aqueous solution has the mass percentage concentration of 10%.
The application method of the seed stem water-retaining agent is characterized in that the seed stem water-retaining agent is used for seed stem treatment before cassava preservation or/and seed stem section treatment before cassava planting.
The using method of the seed stem water retention agent comprises the steps of diluting the seed stem water retention agent with water into a low-concentration water retention agent solution with the concentration of 1% -2%, soaking cassava seed stems in the low-concentration water retention agent solution for 5-30min, taking out and airing.
The application method of the seed stem water-retaining agent comprises the steps of diluting the seed stem water-retaining agent with water into a high-concentration water-retaining agent solution with the concentration of 10% -50%, covering the high-concentration water-retaining agent solution at two ports of the seed stem, and airing.
The using method of the seed stem water retention agent comprises the steps of arranging a plurality of cassava seed stems into bundles, vertically placing the cassava seed stems in a bundle shape, soaking the end parts of the seed stems in a high-concentration water retention agent solution for 15-60 s to a depth of more than or equal to 1cm, taking out the cassava seed stems, treating the other end of the cassava seed stems in the same way, taking out the cassava seed stems and drying the cassava seed stems.
The seed stem water-retaining agent can reduce water dispersion stall of cassava seed stems in the storage process, enhance seed stem nutrients and keep good activity of the seed stems during planting, so that the survival rate of seedlings can be effectively improved, particularly the survival rate in the face of seasonal drought; the cassava yield reduction caused by water shortage during planting is avoided, and the method is particularly suitable for popularization and application in arid areas.
The seed stem water-retaining agent consists of a water-retaining functional substance, an insect repellent substance, an amino acid aqueous solution and a film-forming agent, integrates water retention, disease and pest prevention and nutrition, and is wrapped by the film-forming agent with a protective effect, so that the effective components are fully contacted with the seed stems to play a role, and the loss of water and the effective components is prevented. The polyglutamic acid, the chitosan and the seaweed extract in the water retention functional substance have strong water retention capacity, and the combined action of the polyglutamic acid, the chitosan and the seaweed extract effectively reduces the water loss speed in the storage process. The polyglutamic acid is a biodegradable biological material, and the degraded glutamic acid is easy to absorb and utilize in the growth process of rhizomes and is beneficial to maintaining the activity of seed stems. The chitosan and the seaweed extract are biocompatible high molecular compounds, and molecular chains can be intertwined to form lock cylinder moisture with a net structure. Meanwhile, the seaweed extract contains phytohormones with physiological activity, such as algin, crude protein, various mineral substances and trace elements, so that the water retention property can be improved.
The film forming agent forms a film on the surface of the seed stem, so that the water loss is reduced on the premise of not influencing the respiration of cassava; and the insect repellent substance form a barrier on the surface of the cassava, so that the mildew and the insect pests are reduced. In the process of storing and planting the cassava seed stems, if the cassava seed stem barks are damaged, the damaged parts can have insect pests and mildew risks, and the insect-expelling substances in the seed stem water-retaining agent can kill pests and inhibit microorganisms, so that the cassava is protected to have the effects of expelling insects and preventing mildew, the seed stem quality is improved, the germination of the cassava seeds is promoted, and the seedling emergence amount of cassava seedlings is increased.
Most of raw materials in the seed stem water-retaining agent are non-toxic and harmless biological agents and only contain a small amount of low-toxicity chemical products, so that the production is not limited by the toxic chemical products; and the seed stem water-retaining agent has simple preparation process, convenient operation and easy large-scale production. The medicament is safe in the transportation and use process, has little harm to human body and is suitable for the use of planters.
Detailed Description
The present invention will be described in further detail with reference to examples.
The seed stem water-retaining agent comprises the following components in parts by weight: 0.1 to 10.0 parts of polyglutamic acid, 0.1 to 10.0 parts of chitosan, 1.0 to 10.0 parts of seaweed extract, 5.0 to 20.0 parts of insect repellent substance, 0.1 to 10.0 parts of amino acid aqueous solution and 30.0 to 60.0 parts of film-forming agent aqueous solution.
The polyglutamic acid is prepared by adopting a liquid fermentation mode, and a fermentation medium comprises: 38-42 g/L of glucose, 1.1-1.3 g/L of ammonium chloride, 2.8-3.2 g/L of yeast powder, 65-70 g/L of L-sodium glutamate, 0.3-0.5 g/L of calcium chloride, 0.2-0.4 g/L of magnesium sulfate, 2.1-2.3 g/L of dipotassium hydrogen phosphate and initial pH of 7-7.4, inoculating bacillus into the prepared culture medium, culturing at 36-38 ℃, fermenting for 72 hours, wherein the content of polyglutamic acid in the fermentation liquid can reach 30 g/L. Chitosan is a commercially available product; the seaweed extract is a commercially available product extracted by an enzymolysis method, wherein the alginic acid content is more than or equal to 20 g/L.
The mass percentage concentration of the amino acid aqueous solution is 10 percent, and the amino acid powder and the pure water are mixed according to the proportion and stirred to be dissolved to obtain the amino acid aqueous solution.
The insect repellent substance is Manihot esculenta Crantz leaching solution prepared by leaching at least one of Manihot esculenta Crantz leaf, stem and root bark with water. The insect repellant is produced by cassava plants, has no influence on the cassava and the environment when being used, avoids the pollution of chemical insect repellents to the environment when utilizing waste plants, and is a green and natural insect repellant product.
The water extract of cassava contains a large amount of soluble substances, wherein cyanogenic glucoside is used as a main insect-repellent component, and the content of cyanogenic glucoside in the leaves and root bark of cassava is high. When the leaves or seed stems of cassava plants are damaged, cyanogenic glucoside contained in the cassava plants can be converted into alcohol cyanide, then hydrocyanic acid is generated by cracking, and the cassava plants contain substances for killing pests and inhibiting microorganisms.
The film forming agent is acrylic acid or 108 glue, the film forming agent is diluted by water to obtain a film forming agent aqueous solution, the film forming agent aqueous solution can be an acrylic acid aqueous solution with the mass percentage concentration of 0.5% or a 108 glue aqueous solution with the concentration of 50%, and the film forming agent aqueous solution is preferably an acrylic acid aqueous solution with the concentration of 0.5%.
The preparation method of the seed stem water retention agent is carried out according to the following steps:
a. preparing an amino acid aqueous solution and a film-forming agent aqueous solution with specified concentrations in advance for later use;
b. adding the seaweed extract into the amino acid aqueous solution with the formula amount under stirring, uniformly mixing, adding polyglutamic acid and chitosan, and stirring for dissolving;
c. picking fresh cassava leaves, stems and root barks, crushing at least one of the leaves, the stems or the root barks by using a low-temperature or ultralow-temperature crusher, and crushing to particles with diameters smaller than 0.5-1cm. Rapidly putting the crushed plant fragments into water of 40-50 ℃, fully stirring for 15-20min, and carrying out suction filtration on the mixture to obtain a cassava leaching solution, namely an insect-expelling substance;
the preparation method comprises the following steps that the stirring speed is 150 r/min, and the mass ratio of the cassava plants to water is 1.
d. And (c) adding the film-forming agent aqueous solution with the formula amount into the mixed solution obtained in the step (b), uniformly stirring, adding the insect-repellent substance with the formula amount, and uniformly stirring to obtain the cassava seed stem water-retaining agent.
The cassava seed stem preservation method applying the seed stem water retention agent comprises three methods, namely a soaking method, a dipping method and a coating method, wherein the soaking method 1 comprises the following steps: selecting cassava with high quality, good commodity, no broken peel and no insect bite and rot as seed potatoes, diluting a seed stem water-retaining agent with water into a low-concentration water-retaining agent solution with the mass percentage concentration of 1% -2%, soaking the whole seed stems of the seed potatoes in the low-concentration water-retaining agent solution for 5-30min, taking out and airing. 2. The coating method is that high concentration water-retaining agent solution is directly coated on the cuts at the two ends of the seed stem of the seed potato and dried. The high-concentration water-retaining agent solution is a water solution of a seed stem water-retaining agent with the concentration of 10% -50%. 3. The dipping method is to prepare a container suitable for containing cassava seed stems, pour high-concentration water-retaining agent solution into the container, vertically place the bundled cassava seed stems in the container, enable the end parts of the seed stems to be dipped in the solution for 15-60 seconds at a depth of more than or equal to 1cm, take out the cassava seed stems, treat the other end part of the seed stems in the same way, and dry the cassava seed stems. The preferred method of treatment is dipping. The cassava seed stems treated by the water-retaining agent can be preserved for a long time, keep moisture and activity, are not easy to mildew and prevent pests.
When cassava is planted, the stored cassava seed stems are cut into 10-15cm stem sections by a knife, and each seed stem section is ensured to have at least 1 bud eye. The seed stem sections can be treated by a soaking method, a soaking method and a coating method respectively, the three treatment methods are the same as the use methods of the water-retaining agent when the seed stems are stored, and the treated seed stem sections are directly planted in the field. The preferred method of treatment is dipping. The seedling survival rate of the planted cassava seed stems treated by the water-retaining agent is obviously improved.
Example 1
The addition amount of each raw material for preparing 100 kg of seed stem water retention agent is as follows: 0.5 kg of polyglutamic acid, 0.5 kg of chitosan, 10kg of seaweed extract, 20kg of insect repellent substance, 10kg of amino acid aqueous solution and 59 kg of film forming agent aqueous solution.
a. Preparing 10% amino acid aqueous solution and 0.5% acrylic acid aqueous solution for later use;
b. adding 10kg of seaweed extract into 10kg of amino acid water solution, stirring uniformly, continuously adding 0.5 kg of polyglutamic acid and 0.5 kg of chitosan, and further stirring for dissolving;
c. crushing fresh cassava leaves and root barks by using a low-temperature crusher, quickly putting crushed plant fragments into warm water at 50 ℃ and stirring for 15min, wherein the mass ratio of the plant fragments to the water is 1;
d. and (c) adding 59 kg of film-forming agent aqueous solution into the mixed solution obtained in the step (b), uniformly stirring, adding 20kg of insect-repellent substance obtained in the step (c), and uniformly stirring to obtain the cassava seed stem water-retaining agent A.
Diluting the prepared seed stem water-retaining agent A with water into a low-concentration water-retaining agent solution with the concentration of 2%, selecting cassava seed stems for storage, and treating the cassava seed stems by a soaking method: soaking the seed stems in low concentration seed stem water-retaining agent solution for 15min, taking out, air drying, and storing in shade dry place. After 1 month, dividing the pretreated seed stems into a plurality of sections according to 15cm per section, and treating by using a dipping method; bundling the seed stem segments, immersing the end of the seed stem segments in a high-concentration water-retaining agent solution with the concentration of 20%, taking out the seed stem segments for 30s, and treating the other end of the seed stem segments in the same way. Planting in a flat planting mode, and placing the cassava roots and stems in sections into the shallow pits. The control group takes cassava seed stems of the same quality, is not treated by the seed stem water-retaining agent, and is stored and planted with the treated seed stems under the same conditions. The germination was observed after one week of soil culture, and the results are shown in Table 1.
TABLE 1 cassava seed stem budding Using Water-retaining agent A
Germination rate of both ends of seed stem | Germination rate of other parts of seed stem | |
Using seed stem water-retaining agent | 65 % | 87 % |
Control group | 62 % | 73 % |
As can be seen from the experiments, the germination rate of the cassava seed stems treated by the seed stem water-retaining agent before storage and planting is improved to a certain extent compared with the cassava seed stems not treated.
Example 2
The addition amount of each raw material for preparing 100 kg of seed stem water retention agent is as follows: 5 kg of polyglutamic acid, 5 kg of chitosan, 10kg of seaweed extract, 20kg of insect repellent substance, 10kg of amino acid aqueous solution and 50 kg of 108 glue aqueous solution.
The seed stem water retention agent B is prepared by mixing the raw materials according to the preparation method of the embodiment 1.
Diluting the prepared seed stem water-retaining agent B with water into seed stem water-retaining agent solutions with concentrations of 1% and 50%, selecting cassava seed stems for storage, dividing the cassava seed stems into four groups, soaking the cassava seed stems in the low-concentration water-retaining agent solution for 20min, taking out and drying in the air; secondly, smearing high-concentration water-retaining agent solution on two ends of the whole cassava seed stems by using brushes; in the third group, two ends of the cassava seed stems are respectively soaked in the high-concentration water-retaining agent for 20 seconds, the other ends of the cassava seed stems are treated in the same way, and the cassava seed stems are taken out and dried; the fourth group was not treated as a control group. And placing the four groups of cassava seed stems in a cool and dry place for storage. After 1 month, the pretreated seed stems were divided into several pieces of 15cm each. The treated seed stem sections and the control group were cultured in soil, and after one week, sprouting was observed, and the results are shown in Table 2.
TABLE 2 cassava seed stem budding Using Water-retaining agent B
Germination rate of both ends of seed stem | Germination rate of other parts of seed stem | |
Seed stem water-retaining agent coating port | 67 % | 90 % |
Seed stem water-retaining agent dipping port | 70% | 91% |
Soaking seed stems in water-retaining agent | 66 % | 85 % |
Control group | 60 % | 69 % |
The experiments show that the germination rate of cassava planted after treatment is improved to a certain extent compared with that of a control group by using the seed stem water-retaining agent for pretreatment before preservation, and the germination rate of cassava is highest by a dipping method in the three treatment methods.
Example 3
The seed stem water-retaining agent B prepared in the example 2 is respectively diluted into seed stem water-retaining agent solutions with the concentration of 1% and 10%, cassava seed stems for storage are selected, and the stored cassava seed stems are divided into a plurality of sections according to the length of 15 cm. Dividing the cassava seed stems into four groups, wherein two groups of seed stems are respectively soaked in water retention agent solutions with two concentrations for 30min, the other group coats two ends of the seed stems with seed stem water retention agent B stock solution, the treated seed stems and a control group are cultured in soil, and the germination condition is observed after one week, and the results are shown in table 3.
TABLE 3 cassava seed stem sprouting behavior with two water-retaining agent concentrations
Budding rate of seed stem section | |
Dope coating | 35 % |
Soaking at 10% concentration | 66 % |
Soaking at 1% concentration | 79 % |
Control group | 69 % |
Experiments show that when seed stems are treated by a coating method, a soaking method and a dipping method, the proper concentration of the seed stem water-retaining agent needs to be selected, and as shown in experiments, the seed stem water-retaining agent with low concentration is suitable for being used in the soaking method.
Claims (9)
1. The seed stem water-retaining agent for cassava is characterized by comprising the following components in parts by weight: 0.1 to 10.0 parts of polyglutamic acid, 0.1 to 10.0 parts of chitosan, 1.0 to 10.0 parts of seaweed extract, 5.0 to 20.0 parts of insect repellent substance, 0.1 to 10.0 parts of amino acid aqueous solution and 30.0 to 60.0 parts of film-forming agent aqueous solution.
2. The seed stem water-retaining agent of claim 1, wherein the insect-repellent substance is a water extract of cassava plants, including leaves, stems or root bark.
3. The seed stem water retaining agent of claim 2, wherein the film forming agent is acrylic acid or 108 glue, and the film forming agent is diluted with water to obtain an aqueous solution of the film forming agent.
4. The seed stem water-retaining agent according to claim 3, wherein the insect-repellent substance is prepared by pulverizing fresh cassava leaves, stems and root barks, and rapidly putting the pulverized plant fragments into warm water for sufficient stirring; and (4) leaching the cassava extract after suction filtration to obtain the insect repellent substance.
5. The seed stem water-retaining agent according to claim 3, wherein the film-forming agent aqueous solution is an acrylic acid aqueous solution with a mass concentration of 0.5% or a 108 glue aqueous solution with a mass concentration of 50%, and the amino acid aqueous solution has a mass percentage concentration of 10%.
6. A use method of the water-retaining agent for seed stems as claimed in any one of claims 1 to 5, wherein the water-retaining agent for seed stems is used for seed stem treatment before cassava preservation or/and seed stem segment treatment before cassava planting.
7. The use method of the seed stem water-retaining agent as claimed in claim 6, wherein the seed stem water-retaining agent is diluted with water to form a low concentration water-retaining agent solution with a concentration of 1% -2%, the cassava seed stem is soaked in the low concentration water-retaining agent solution for 5-30min, and then the cassava seed stem is taken out and dried.
8. The use method of the seed stem water-retaining agent as claimed in claim 6, wherein the seed stem water-retaining agent is diluted with water to form a high concentration water-retaining agent solution with a concentration of 10% -50%, and the two ports of the seed stem are covered with the high concentration water-retaining agent solution and dried.
9. The use method of the seed stem water-retaining agent as claimed in claim 8, wherein a plurality of cassava seed stems are arranged into bundles and placed vertically, the end parts of the seed stems are soaked in the high-concentration water-retaining agent solution for a depth of 1cm or more, the seed stems are taken out after being soaked for 15-60 s, the other end of the seed stems are treated the same way, and the seed stems are taken out and dried.
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