CN115812098A - 具有主动补偿的电穿孔 - Google Patents

具有主动补偿的电穿孔 Download PDF

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Publication number
CN115812098A
CN115812098A CN202180049496.XA CN202180049496A CN115812098A CN 115812098 A CN115812098 A CN 115812098A CN 202180049496 A CN202180049496 A CN 202180049496A CN 115812098 A CN115812098 A CN 115812098A
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CN
China
Prior art keywords
electrodes
fluid
resistance
value
power supply
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202180049496.XA
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English (en)
Chinese (zh)
Inventor
张志玮
J·T·范卢
A·梅赫拉比
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Immunitybio Inc
Original Assignee
NantCell Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by NantCell Inc filed Critical NantCell Inc
Publication of CN115812098A publication Critical patent/CN115812098A/zh
Pending legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/08—Arrangements or circuits for monitoring, protecting, controlling or indicating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0472—Structure-related aspects
    • A61N1/0476—Array electrodes (including any electrode arrangement with more than one electrode for at least one of the polarities)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/372—Arrangements in connection with the implantation of stimulators
    • A61N1/378—Electrical supply
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits

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  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Radiology & Medical Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
CN202180049496.XA 2020-07-22 2021-07-15 具有主动补偿的电穿孔 Pending CN115812098A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/935987 2020-07-22
US16/935,987 US12006507B2 (en) 2020-07-22 2020-07-22 Electroporation with active compensation
PCT/US2021/041832 WO2022020175A1 (en) 2020-07-22 2021-07-15 Electroporation with active compensation

Publications (1)

Publication Number Publication Date
CN115812098A true CN115812098A (zh) 2023-03-17

Family

ID=77367476

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202180049496.XA Pending CN115812098A (zh) 2020-07-22 2021-07-15 具有主动补偿的电穿孔

Country Status (6)

Country Link
US (2) US12006507B2 (https=)
EP (1) EP4185686A1 (https=)
JP (1) JP7742874B2 (https=)
KR (1) KR102913102B1 (https=)
CN (1) CN115812098A (https=)
WO (1) WO2022020175A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD1016324S1 (en) 2020-07-08 2024-02-27 NanoCav, LLC Biological cell processing chip
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
CN117715929A (zh) 2021-07-29 2024-03-15 南特细胞公司 用于预防和治疗病毒感染和癌症的经修饰的t细胞受体
CN115449481A (zh) * 2022-09-26 2022-12-09 杭州维纳安可医疗科技有限责任公司 电转染装置及方法

Family Cites Families (26)

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US5545130A (en) 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US6387671B1 (en) 1999-07-21 2002-05-14 The Regents Of The University Of California Electrical impedance tomography to control electroporation
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US6403348B1 (en) 1999-07-21 2002-06-11 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
AU2002326717A1 (en) 2001-08-22 2003-03-10 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7245963B2 (en) 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US6801804B2 (en) 2002-05-03 2004-10-05 Aciont, Inc. Device and method for monitoring and controlling electrical resistance at a tissue site undergoing iontophoresis
WO2006112870A1 (en) 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
CA2666501C (en) 2006-10-17 2017-03-21 Vgx Pharmaceuticals, Inc. Electroporation devices and methods of using same for electroporation of cells in mammals
US8222909B2 (en) 2008-08-22 2012-07-17 Bio-Rad Laboratories, Inc. Electroporation system with dial-in time constant
CN101857836B (zh) 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 一种流式电穿孔装置及系统
CN203451533U (zh) 2013-08-27 2014-02-26 苏州文曲生物微系统有限公司 一种基于新型微电极芯片的电穿孔系统
US10488391B2 (en) * 2014-10-31 2019-11-26 The Regents Of The University Of California Neural circuit probe
ES2807439T3 (es) * 2015-03-31 2021-02-23 Oncosec Medical Inc Sistemas para electroporación mejorada basada en la detección de tejidos
BR112018004278B1 (pt) 2015-09-04 2022-05-03 Rutgers, The State University Of New Jersey Sistema para eletroporação de uma célula biológica, e método para eletroporação de células biológicas em uma solução tampão
ES2687422T3 (es) * 2015-09-07 2018-10-25 Miltenyi Biotec Gmbh Cartucho desechable para electroporación
US11225638B2 (en) 2016-04-04 2022-01-18 CyteQuest, Inc. System, device and method for electroporation of cells
US20170291172A1 (en) 2016-04-11 2017-10-12 The Regents Of The University Of California Tunable, sheathless, and three dimensional single-stream cell focusing and sorting in high speed flows
EP4613776A3 (en) 2016-10-21 2025-11-26 Altor BioScience Corporation Multimeric il-15-based molecules
WO2018094249A1 (en) 2016-11-18 2018-05-24 NanoCav, LLC Methods of sorting activated t cells using three dimensional dielectrophoresis
JP6985508B2 (ja) * 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
US20190292510A1 (en) * 2018-03-20 2019-09-26 The Charles Stark Draper Laboratory, Inc. Dual-Purpose Viral Transduction and Electroporation Device
WO2020014264A1 (en) 2018-07-09 2020-01-16 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
EP3915633A1 (en) * 2020-05-29 2021-12-01 Imec VZW Control of cell electroporation
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation

Also Published As

Publication number Publication date
US12571003B2 (en) 2026-03-10
US20240229074A1 (en) 2024-07-11
JP7742874B2 (ja) 2025-09-22
KR102913102B1 (ko) 2026-01-14
EP4185686A1 (en) 2023-05-31
KR20230038733A (ko) 2023-03-21
JP2023537253A (ja) 2023-08-31
US12006507B2 (en) 2024-06-11
US20220025402A1 (en) 2022-01-27
WO2022020175A1 (en) 2022-01-27

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