KR102913102B1 - 능동 보상을 사용하는 전기천공(electroporation with active compensation) - Google Patents

능동 보상을 사용하는 전기천공(electroporation with active compensation)

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Publication number
KR102913102B1
KR102913102B1 KR1020237004497A KR20237004497A KR102913102B1 KR 102913102 B1 KR102913102 B1 KR 102913102B1 KR 1020237004497 A KR1020237004497 A KR 1020237004497A KR 20237004497 A KR20237004497 A KR 20237004497A KR 102913102 B1 KR102913102 B1 KR 102913102B1
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South Korea
Prior art keywords
electrodes
fluid
resistance
value
current
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KR1020237004497A
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English (en)
Korean (ko)
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KR20230038733A (ko
Inventor
치-웨이 창
존 토마스 반 류
아틴 메라비
Original Assignee
난트셀, 인크.
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Publication of KR20230038733A publication Critical patent/KR20230038733A/ko
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M35/00—Means for application of stress for stimulating the growth of microorganisms or the generation of fermentation or metabolic products; Means for electroporation or cell fusion
    • C12M35/02—Electrical or electromagnetic means, e.g. for electroporation or for cell fusion
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/08—Arrangements or circuits for monitoring, protecting, controlling or indicating
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M41/00—Means for regulation, monitoring, measurement or control, e.g. flow regulation
    • C12M41/48—Automatic or computerized control
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • H—ELECTRICITY
    • H03—ELECTRONIC CIRCUITRY
    • H03K—PULSE TECHNIQUE
    • H03K3/00—Circuits for generating electric pulses; Monostable, bistable or multistable circuits
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/02—Details
    • A61N1/04—Electrodes
    • A61N1/0404—Electrodes for external use
    • A61N1/0472—Structure-related aspects
    • A61N1/0476—Array electrodes (including any electrode arrangement with more than one electrode for at least one of the polarities)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61N—ELECTROTHERAPY; MAGNETOTHERAPY; RADIATION THERAPY; ULTRASOUND THERAPY
    • A61N1/00—Electrotherapy; Circuits therefor
    • A61N1/18—Applying electric currents by contact electrodes
    • A61N1/32—Applying electric currents by contact electrodes alternating or intermittent currents
    • A61N1/36—Applying electric currents by contact electrodes alternating or intermittent currents for stimulation
    • A61N1/372—Arrangements in connection with the implantation of stimulators
    • A61N1/378—Electrical supply

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Sustainable Development (AREA)
  • Electromagnetism (AREA)
  • Cell Biology (AREA)
  • Analytical Chemistry (AREA)
  • Plant Pathology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Neurology (AREA)
  • Heart & Thoracic Surgery (AREA)
  • Radiology & Medical Imaging (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Computer Hardware Design (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
KR1020237004497A 2020-07-22 2021-07-15 능동 보상을 사용하는 전기천공(electroporation with active compensation) Active KR102913102B1 (ko)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16/935,987 2020-07-22
US16/935,987 US12006507B2 (en) 2020-07-22 2020-07-22 Electroporation with active compensation
PCT/US2021/041832 WO2022020175A1 (en) 2020-07-22 2021-07-15 Electroporation with active compensation

Publications (2)

Publication Number Publication Date
KR20230038733A KR20230038733A (ko) 2023-03-21
KR102913102B1 true KR102913102B1 (ko) 2026-01-14

Family

ID=77367476

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1020237004497A Active KR102913102B1 (ko) 2020-07-22 2021-07-15 능동 보상을 사용하는 전기천공(electroporation with active compensation)

Country Status (6)

Country Link
US (2) US12006507B2 (https=)
EP (1) EP4185686A1 (https=)
JP (1) JP7742874B2 (https=)
KR (1) KR102913102B1 (https=)
CN (1) CN115812098A (https=)
WO (1) WO2022020175A1 (https=)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD1016324S1 (en) 2020-07-08 2024-02-27 NanoCav, LLC Biological cell processing chip
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation
CN117715929A (zh) 2021-07-29 2024-03-15 南特细胞公司 用于预防和治疗病毒感染和癌症的经修饰的t细胞受体
CN115449481A (zh) * 2022-09-26 2022-12-09 杭州维纳安可医疗科技有限责任公司 电转染装置及方法

Citations (3)

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WO2006112870A1 (en) 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US20170241976A1 (en) 2014-10-31 2017-08-24 The Regents Of The University Of California Neural circuit probe
US20190292510A1 (en) 2018-03-20 2019-09-26 The Charles Stark Draper Laboratory, Inc. Dual-Purpose Viral Transduction and Electroporation Device

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US5545130A (en) 1992-04-08 1996-08-13 Genetronics, Inc. Flow through electroporation method
US6387671B1 (en) 1999-07-21 2002-05-14 The Regents Of The University Of California Electrical impedance tomography to control electroporation
US6300108B1 (en) 1999-07-21 2001-10-09 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US6403348B1 (en) 1999-07-21 2002-06-11 The Regents Of The University Of California Controlled electroporation and mass transfer across cell membranes
US7029916B2 (en) 2001-02-21 2006-04-18 Maxcyte, Inc. Apparatus and method for flow electroporation of biological samples
AU2002326717A1 (en) 2001-08-22 2003-03-10 Maxcyte, Inc. Apparatus and method for electroporation of biological samples
US7245963B2 (en) 2002-03-07 2007-07-17 Advisys, Inc. Electrode assembly for constant-current electroporation and use
US6801804B2 (en) 2002-05-03 2004-10-05 Aciont, Inc. Device and method for monitoring and controlling electrical resistance at a tissue site undergoing iontophoresis
CA2666501C (en) 2006-10-17 2017-03-21 Vgx Pharmaceuticals, Inc. Electroporation devices and methods of using same for electroporation of cells in mammals
US8222909B2 (en) 2008-08-22 2012-07-17 Bio-Rad Laboratories, Inc. Electroporation system with dial-in time constant
CN101857836B (zh) 2009-11-10 2013-06-12 昆山文曲生物微系统有限公司 一种流式电穿孔装置及系统
CN203451533U (zh) 2013-08-27 2014-02-26 苏州文曲生物微系统有限公司 一种基于新型微电极芯片的电穿孔系统
ES2807439T3 (es) * 2015-03-31 2021-02-23 Oncosec Medical Inc Sistemas para electroporación mejorada basada en la detección de tejidos
BR112018004278B1 (pt) 2015-09-04 2022-05-03 Rutgers, The State University Of New Jersey Sistema para eletroporação de uma célula biológica, e método para eletroporação de células biológicas em uma solução tampão
ES2687422T3 (es) * 2015-09-07 2018-10-25 Miltenyi Biotec Gmbh Cartucho desechable para electroporación
US11225638B2 (en) 2016-04-04 2022-01-18 CyteQuest, Inc. System, device and method for electroporation of cells
US20170291172A1 (en) 2016-04-11 2017-10-12 The Regents Of The University Of California Tunable, sheathless, and three dimensional single-stream cell focusing and sorting in high speed flows
EP4613776A3 (en) 2016-10-21 2025-11-26 Altor BioScience Corporation Multimeric il-15-based molecules
WO2018094249A1 (en) 2016-11-18 2018-05-24 NanoCav, LLC Methods of sorting activated t cells using three dimensional dielectrophoresis
JP6985508B2 (ja) * 2017-09-30 2021-12-22 インスクリプタ， インコーポレイテッド フロースルーエレクトロポレーション機器編成
WO2020014264A1 (en) 2018-07-09 2020-01-16 NanoCav, LLC Micro flow-through electroporation devices and methods of cell transfection
EP3915633A1 (en) * 2020-05-29 2021-12-01 Imec VZW Control of cell electroporation
US12006507B2 (en) 2020-07-22 2024-06-11 Nantcell, Inc. Electroporation with active compensation

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2006112870A1 (en) 2005-04-19 2006-10-26 Excellin Life Sciences, Inc. Device and method for controlled electroporation and molecular delivery in cells and tissue
US20170241976A1 (en) 2014-10-31 2017-08-24 The Regents Of The University Of California Neural circuit probe
US20190292510A1 (en) 2018-03-20 2019-09-26 The Charles Stark Draper Laboratory, Inc. Dual-Purpose Viral Transduction and Electroporation Device

Also Published As

Publication number Publication date
US12571003B2 (en) 2026-03-10
US20240229074A1 (en) 2024-07-11
JP7742874B2 (ja) 2025-09-22
EP4185686A1 (en) 2023-05-31
KR20230038733A (ko) 2023-03-21
JP2023537253A (ja) 2023-08-31
US12006507B2 (en) 2024-06-11
US20220025402A1 (en) 2022-01-27
WO2022020175A1 (en) 2022-01-27
CN115812098A (zh) 2023-03-17

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