CN115779008A - Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method and application thereof - Google Patents

Abstract

The invention provides a traditional Chinese medicine composition for preventing and treating chicken colibacillosis and a preparation method and application thereof, belonging to the field of veterinary medicines, and the active ingredients comprise the following raw materials in parts by weight: 20 to 40 portions of Japanese thistle herb, 10 to 30 portions of mulberry leaf, 10 to 30 portions of isatis root, 5 to 15 portions of largehead atractylodes rhizome, 5 to 15 portions of hawthorn and 5 to 10 portions of liquorice. The traditional Chinese medicine composition disclosed by the invention is mild in medicine property, has the effects of clearing away heat and toxic materials, eliminating dampness and removing stasis, tonifying spleen and qi and preventing and controlling diseases, and is a traditional Chinese veterinary medicine preparation which can effectively replace antibiotic and good in antibacterial effect and is used for treating chicken colibacillosis.

Description

Traditional Chinese medicine composition for preventing and treating chicken colibacillosis and preparation method and application thereof

Technical Field

The invention relates to the technical field of veterinary medicines, and particularly relates to a traditional Chinese medicine composition for preventing and treating chicken colibacillosis and a preparation method and application thereof.

Background

Colibacillosis in chickens is an avian infection caused by different serotypes of pathogenic escherichia coli. The infection routes of the disease include oral transmission, egg transmission, respiratory transmission and cloacal infection. Chickens of various ages can become infected and can occur throughout the year. The Escherichia coli has super-strong resistance to environment, and can be attached to feces, dust, chicken villi, broken egg skin, etc. The clinical manifestations of the disease are very complex and diverse, and the most serious of them is acute septicemia, and of course, vitelline peritonitis, pericarditis, perihepatitis, synovitis and genital diseases. In particular, in recent years, colibacillosis in chickens has been widely prevalent in many chicken farms, and the morbidity and mortality rates are high, and in addition, colibacillosis is extremely prone to generate drug resistance to antibiotic drugs, so that the treatment cost is increased, and immeasurable economic loss is caused to the poultry industry. Colibacillosis in chickens seriously threatens the healthy development of the poultry industry. Effectively preventing and treating chicken colibacillosis becomes a difficult problem to be solved urgently.

Colibacillosis in chickens is a conditioned disease, and the occurrence and prevalence of the disease can be promoted by severe external environmental conditions and various stress factors. Stress factors such as sudden change of climate, sudden cooling, damp heat, poor ventilation effect, excessive ammonia taste, moldy feed, lack of nutrition, improper use of antibacterial drugs and the like weaken the disease resistance of chicken flocks, and various pathogenic microorganisms invade the body by the opportunity to cause morbidity. Chinese medicine dialectical observation is carried out by observing the clinical symptoms of chicken suffering from colibacillosis; after the chicken group suffers from illness, the spirit is low, the mouth of the chicken is not wet, the coronaria is cyanotic, most of the feces of the chicken are yellow white or yellow green feces, and the feces are thin first and then dry, which indicates that most of the feces of the sick chicken are hot during the disease period. The first thing is that the dead chicken can find pericardial hepatitis, and the liver can be blood stasis swelling or bleeding, the eyes and the whole head are swollen, yellow liquid and cellulose are exuded under the skin, the white or light yellow color is formed, and the intestinal mucosa is congested or ruptured and bled on the intestinal tract to form enteritis, which can indicate the manifestations of yang fire flaming up, no diuresis, three-jiao excessive heat and body fluid exudation and stagnation caused by excessive internal heat. According to the observation of the clinical symptoms of chicken infected by escherichia coli, the disease of the chicken is caused by the invasion of exogenous pathogenic factors to cause heat-dampness and stasis of the organism. The exterior pathogens should be removed first, but the treatment time is too wrong, so the interior deficiency and damp-heat will go on deeply, and the damp-heat will enter the large intestine of yangming syndrome and the large intestine will conduct abnormally.

The antibiotics for preventing and treating chicken colibacillosis clinically used at present are easy to generate residues, have large drug resistance and side effects, are high in use cost, and the used traditional Chinese medicine composition has the defects of unscientific prescription composition, improper dosage, unsatisfactory treatment effect, easy relapse and the like. The disease caused by chicken colibacillus is treated by integrally looking at the pathogenic mechanism and the dialectical symptoms of chicken colibacillus from the traditional Chinese medicine perspective, rather than simply aiming at treating or killing the colibacillus. The traditional Chinese medicine composition for preventing and treating the chicken colibacillosis with good clinical effect and high use cost ratio is screened out by combining the morbidity characteristics of the current chicken colibacillosis.

Disclosure of Invention

Object of the Invention

The invention aims to provide the traditional Chinese medicine composition for preventing and treating chicken colibacillosis, which has mild medicine property, is capable of clearing heat and removing toxicity, eliminating dampness and removing blood stasis, tonifying spleen and qi, and preventing and controlling diseases, effectively replaces antibiotics and has good antibacterial effect, and the preparation method and the application thereof.

Solution scheme

In order to realize the purpose of the invention, the technical scheme adopted by the invention is as follows:

in a first aspect, the invention provides a traditional Chinese medicine composition for preventing and treating chicken colibacillosis, which comprises the following raw materials in parts by weight: 20 to 40 parts of Japanese thistle herb, 10 to 30 parts of mulberry leaf, 10 to 30 parts of isatis root, 5 to 15 parts of largehead atractylodes rhizome, 5 to 15 parts of hawthorn and 5 to 10 parts of liquorice.

The Chinese medicinal composition has the following medicinal properties and effects:

herba seu radix Cirsii Japonici; cold in nature, sweet and bitter in taste, entering heart and liver meridians. Has effects of cooling blood, stopping bleeding, removing blood stasis, relieving swelling, removing toxic substance, and resolving carbuncle. Modern pharmacological research believes that the Japanese thistle herb has the effects of reducing blood pressure, protecting liver and benefiting gallbladder, removing jaundice, resisting bacteria, stopping bleeding, cooling blood and relieving pain, promoting blood circulation to remove blood stasis, reducing swelling, resisting bacteria and diminishing inflammation and the like.

Mulberry leaves; sweet, bitter and cold. It enters lung and liver meridians. Dispelling pathogenic wind, clearing heat, clearing lung-heat, moistening dryness, removing liver fire, and improving eyesight. Modern pharmacological researches believe that mulberry leaves also have the effects of reducing blood sugar, activating blood circulation to dissipate blood stasis, protecting liver function, improving sleep and relieving the symptoms of lung pain.

Radix Isatidis; cold in nature, bitter in taste, entering heart and stomach meridians. Has effects of clearing away heat and toxic materials, cooling blood, and relieving sore throat. Modern pharmacological research believes that the isatis root has an antibacterial effect, an anti-influenza virus effect, a hepatitis virus effect and the like, and a remarkable anti-leukemia effect and the like.

Largehead atractylodes rhizome; warm in nature, bitter and sweet in flavor, entering spleen and stomach meridians. Invigorating spleen, invigorating qi, eliminating dampness, inducing diuresis, arresting sweating, regulating stomach, and preventing miscarriage. Rhizoma Atractylodis Macrocephalae has effects in promoting joint movement, removing dampness, and eliminating edema. Modern pharmacological research believes that the bighead atractylodes rhizome has the effects of promoting gastrointestinal motility, protecting liver and benefiting gallbladder, resisting oxidation, reducing blood sugar, resisting coagulation, resisting bacteria, dilating blood vessels, inhibiting heart, calming and the like. In addition, the traditional Chinese medicine composition also has obvious and durable diuretic effect and obvious inhibition effect on various cancer cells. Has bidirectional regulating effect on gastrointestinal system, and can be used for treating gastric ulcer.

Hawthorn fruit; sour and sweet in flavor, slightly warm in nature, entering spleen, stomach and liver meridians. Promoting digestion, eliminating food stagnation, promoting qi circulation, and removing blood stasis. In addition, it has actions of moving qi and alleviating pain and treating dysentery, so it is indicated for abdominal pain and hernia due to diarrhea and dysentery.

Licorice root; sweet and neutral. It enters heart, lung, spleen and stomach meridians. Invigorating spleen and replenishing qi, clearing away heat and toxic material, eliminating phlegm and relieving cough, relieving spasm and pain, and harmonizing the drugs. Modern pharmacological studies have suggested that licorice has anti-inflammatory, antiviral, and antiallergic effects, and it protects the mucous membranes of the inflamed throat and trachea.

In the formula, the circium japonicum is pungent and sweet in flavor and cool in nature, enters heart and liver meridians, can cool blood and relieve pain, remove stasis and detumescence, and has antibacterial and anti-inflammatory effects, so that the circium japonicum is a monarch drug. The radix isatidis is matched to have the effects of clearing away heat and toxic materials, cooling blood and relieving sore throat, and can purge the fire of heart and stomach; the mulberry leaves can play the roles of dispelling wind, clearing heat, clearing lung, moistening dryness, clearing liver and improving vision, and clearing and nourishing lung and liver, and the mulberry leaves are used as ministerial drugs. The main effects of the atractylodes macrocephala koidz are strengthening the spleen and replenishing qi, eliminating dampness and inducing diuresis; hawthorn fruit, fructus crataegi, has the effects of promoting digestion, relieving stasis, promoting qi circulation and removing blood stasis, and both of them are used as adjuvant drugs. Licorice root, radix Glycyrrhizae is a guiding drug because it can tonify spleen and qi, clear away heat and toxic materials, dispel phlegm and relieve cough, relieve spasm and pain, and harmonize the effects of the other drugs.

The traditional Chinese medicine composition can achieve the best drug effect in a synergistic manner, and provides a reference basis for treating chicken colibacillosis by using traditional Chinese veterinary medicines. The traditional Chinese medicine composition disclosed by the invention is mild in medicine property, has the effects of clearing away heat and toxic materials, eliminating dampness and removing stasis, tonifying spleen and qi, and preventing and controlling diseases, and can effectively replace a traditional Chinese veterinary medicine preparation which is biological-resistant and has a good antibacterial effect and is used for treating chicken colibacillosis and a preparation method thereof.

Further, the feed comprises the following raw materials in parts by weight: 20 to 40 portions of Japanese thistle herb, 15 to 30 portions of mulberry leaf, 15 to 30 portions of isatis root, 5 to 15 portions of largehead atractylodes rhizome, 5 to 15 portions of hawthorn and 5 to 10 portions of liquorice.

Further, the feed comprises the following raw materials in parts by weight: 30 to 40 portions of Japanese thistle herb, 20 to 30 portions of mulberry leaf, 20 to 30 portions of isatis root, 10 to 15 portions of largehead atractylodes rhizome, 10 to 15 portions of hawthorn and 8 to 10 portions of liquorice.

Optionally, the feed comprises the following raw materials in parts by weight: 30 to 35 parts of Japanese thistle herb, 20 to 25 parts of mulberry leaf, 20 to 25 parts of isatis root, 10 to 15 parts of largehead atractylodes rhizome, 10 to 15 parts of hawthorn and 8 to 10 parts of liquorice.

Optionally, the feed comprises the following raw materials in parts by weight: 30 parts of Japanese thistle herb, 20 parts of mulberry leaf, 20 parts of isatis root, 10 parts of bighead atractylodes rhizome, 10 parts of hawthorn and 8 parts of liquorice.

Furthermore, the composition can be prepared into any one of injection, powder, capsules, tablets and oral liquid.

Furthermore, the dosage form of the composition is oral liquid.

Further, the crude drug amount of the oral liquid is 0.5-2 g/mL, optionally 1g/mL.

Further, the oral liquid is prepared by decocting and concentrating the traditional Chinese medicine composition.

In a second aspect, a preparation method of the traditional Chinese medicine composition is provided, which comprises the following steps: the medicine is prepared by taking the raw materials of the medicinal materials according to the parts by weight, crushing, decocting and concentrating.

Further, the method comprises the following steps:

1) Taking the medicinal materials of the traditional Chinese medicine composition according to the weight parts, crushing and sieving; sieving with a second sieve;

2) Weighing the raw materials sieved in the step 1), adding water with the weight 9-11 times of the total weight, soaking the raw materials for 0.5-1 h before decocting, and decocting for 1-3 h;

3) Filtering the decocted traditional Chinese medicine in the step 2), adding water 7-9 times of the total weight of the medicinal materials into filter residues, and decocting for 1-2 hours;

4) Filtering the decocted traditional Chinese medicine in the step 3), adding water which is 6-8 times of the total weight of the medicinal materials, and decocting for 1-2 hours;

5) Mixing the filtered liquid medicines in the steps 2), 3) and 4), concentrating the liquid medicine until the crude drug amount is 1g/mL, and sterilizing to prepare the oral liquid.

The invention also provides application of the traditional Chinese medicine composition for preventing chicken colibacillosis, which is used for treating chicken colibacillosis, wherein the chicken colibacillosis comprises various diseases such as colibacillary pericarditis, perihepatitis, peritonitis, salpingitis, omphalitis, synovitis, air sacculitis, granuloma, ophthalmia and the like.

Optionally, the chicken colibacillosis is chicken sepsis type colibacillosis.

Advantageous effects

The traditional Chinese medicine composition can achieve the best drug effect in a synergistic manner, and provides a reference basis for treating chicken colibacillosis by using traditional Chinese veterinary medicines. The traditional Chinese medicine composition disclosed by the invention is mild in medicine property, has the effects of clearing away heat and toxic materials, eliminating dampness and removing stasis, tonifying spleen and qi and preventing and controlling diseases, and is a traditional Chinese veterinary medicine preparation which can effectively replace antibiotic and good in antibacterial effect and is used for treating chicken colibacillosis.

Drawings

One or more embodiments are illustrated by the corresponding figures in the drawings, which are not meant to be limiting. The word "exemplary" is used exclusively herein to mean "serving as an example, embodiment, or illustration. Any embodiment described herein as "exemplary" is not necessarily to be construed as preferred or advantageous over other embodiments.

FIG. 1 anatomical pictures of healthy chickens in the placebo treatment trial of the test examples of the present invention.

FIG. 2 is an anatomical picture of a control group of animals treated with the model of the present invention.

FIG. 3 is an anatomical picture of a control group of herbal groups of the present invention.

FIG. 4 anatomical pictures of the challenged chickens of the group administered in example 1 in the treatment trial of the test examples of the present invention.

FIG. 5 is a picture of the clinical symptoms of challenge chickens in the test examples of the invention.

Detailed Description

In order to make the objects, technical solutions and advantages of the embodiments of the present invention clearer, the technical solutions in the embodiments of the present invention will be clearly and completely described below, and it is obvious that the described embodiments are some, but not all embodiments of the present invention. All other embodiments, which can be obtained by a person skilled in the art without inventive step based on the embodiments of the present invention, are within the scope of protection of the present invention.

Furthermore, in the following detailed description, numerous specific details are set forth in order to provide a better understanding of the present invention. It will be understood by those skilled in the art that the present invention may be practiced without some of these specific details. In some embodiments, materials, elements, methods, means, and the like that are well known to those skilled in the art are not described in detail in order to not unnecessarily obscure the present invention.

Example 1

3kg of Japanese thistle herb, 2kg of mulberry leaf, 2kg of isatis root, 1kg of bighead atractylodes rhizome, 1kg of hawthorn fruit and 0.8kg of liquorice.

Example 2

2kg of Japanese thistle herb, 2kg of mulberry leaf, 2kg of isatis root, 0.5kg of bighead atractylodes rhizome, 0.5kg of hawthorn fruit and 0.5kg of liquorice.

Example 3

4kg of Japanese thistle herb, 3kg of mulberry leaf, 3kg of isatis root, 1.5kg of bighead atractylodes rhizome, 1.5kg of hawthorn fruit and 1kg of liquorice.

Example 4

4kg of Japanese thistle herb, 1.5kg of mulberry leaf, 1.5kg of isatis root, 1kg of bighead atractylodes rhizome, 1kg of hawthorn fruit and 0.8kg of liquorice.

Example 5

2kg of Japanese thistle herb, 1.5kg of mulberry leaf, 1.5kg of isatis root, 1kg of bighead atractylodes rhizome, 1kg of hawthorn fruit and 0.8kg of liquorice.

Comparative example 1

The difference between the present example and example 1 lies in the difference between the raw materials and the mixture ratio: 1kg of Japanese thistle herb, 1.5kg of mulberry leaf, 1.5kg of isatis root, 1kg of bighead atractylodes rhizome, 1kg of hawthorn fruit and 0.8kg of liquorice.

Comparative example 2

The difference between the present example and example 1 lies in the difference between the raw materials and the mixture ratio: 5kg of Japanese thistle herb, 1.5kg of mulberry leaf, 1.5kg of isatis root, 1kg of bighead atractylodes rhizome, 1kg of hawthorn fruit and 0.8kg of liquorice.

Comparative example 3

The difference between the embodiment and the embodiment 1 is that the radix isatidis is replaced by dandelion: 3kg of Japanese thistle herb, 2kg of mulberry leaf, 2kg of dandelion, 1kg of bighead atractylodes rhizome, 1kg of hawthorn and 0.8kg of liquorice.

Comparative example 4

The difference between the embodiment and the embodiment 1 is that the mulberry leaves are replaced by chrysanthemum: 3kg of Japanese thistle herb, 2kg of chrysanthemum, 2kg of isatis root, 1kg of bighead atractylodes rhizome, 1kg of hawthorn and 0.8kg of liquorice.

Comparative example 5

The difference between the present example and example 1 is that the raw material is removed of atractylodes: 3kg of Japanese thistle herb, 2kg of mulberry leaf, 2kg of isatis root, 1kg of hawthorn and 0.8kg of liquorice.

Preparation of medicinal solutions

1) The raw materials of the above examples 1 to 5 and comparative examples 1 to 5 were mixed in the ratio. Pulverizing and sieving; and sieving the mixture by a second sieve.

2) Weighing the raw material medicine sieved in the step 1), adding water with the weight being 9-11 times of the total weight, soaking the raw material medicine for 0.5h before decocting, and decocting for 1.5h;

3) Filtering the traditional Chinese medicine in the step 2), adding water which is 7-9 times of the total weight of the medicinal materials into filter residues, and decocting for 1 hour;

4) Filtering the traditional Chinese medicine in the step 3), adding water which is 6-8 times of the total weight of the medicinal materials, and decocting for 1 hour;

5) Combining the liquid medicines decocted for three times in the steps 2), 3) and 4), standing overnight, centrifuging to obtain supernate, concentrating the liquid medicines until the crude drug amount is 1g/mL, sterilizing to respectively prepare the oral liquid of the embodiments 1-5 and the oral liquid of the comparative examples 1-5, and refrigerating at 2-10 ℃ for later use.

Test example: establishment of chicken colibacillosis model and development of prevention and treatment test

1.1 challenge dosimetry

Material

1 day old broiler chickens purchased from Beijing Dafeng poultry Breeding Co.

Nutrient broth and nutrient agar, both purchased from the biotechnology limited of the beijing zhonghai.

The method for determining the median lethal dose comprises the following steps: the test chickens are raised in cages by routine, freely drinking water and collecting food to two weeks of age, the abnormal defective chickens are removed before grouping, the test is divided into 4 groups, each group comprises 12 chickens, and 10 prepared above are used ⁸ Bacterial solution at cfu/mL concentration. Except for the blank group, which was injected with 0.7mL of physiological saline, each chicken was injected with 0.6mL, 0.7mL, and 0.8mL of bacterial suspension by pectoralis injection. Sufficient drinking water and antibiotic-free additive feed are given. 3h after the attack of the toxin shows that the spirit is depressed and the disease is cachectic, 12h later shows that dead chickens exist, 120h after the attack of the toxin is observed, the death number of all groups of chickens is counted, and the half-death number is calculated by using a Bliss methodAnd counting the death rate, and counting the morbidity and mortality.

The test results of half lethal dose determination are shown in the following table 1, wherein the incidence rate of a pectoralis injection group with 0.7mL is 100%, the death percentage is 58.33%, and the toxic counteracting dose of a bacterial liquid effect test with 0.7mL is selected.

TABLE 1 determination of challenge dose for colibacillosis in chickens

1.2 animal experiments

The test chicken flocks are normally raised in cages, free drinking water and food intake are carried out until the test chicken flocks are two weeks old, and the defective and weak abnormal chickens are removed before grouping.

Grouping prevention tests; the random method is divided into 10 groups on average, which are blank control group 1, chinese medicine compound group 8 (respectively fed with the Chinese medicine oral liquid of examples 1-3 and comparative examples 1-5) and model control group 1, wherein each group contains 36 animals, each group contains 3 repeat groups, and each repeat group contains 12 animals. The detailed grouping and administration schedule of the test chickens in each group are shown in Table 2.

Grouping treatment tests; the random method was divided into 11 groups, which were blank control group 1, chinese herbal compound group 8 (fed with the Chinese herbal oral liquid of examples 1-3 and comparative examples 1-5), drug control group 1, and model control group 1, respectively, with 36 animals per group, 3 repeat groups per group, and 12 animals per repeat group. The detailed grouping and administration schedule of the test chickens in each group are shown in Table 2.

TABLE 2 preventive test and therapeutic test group of chickens treatment method

Note: the drug control group is prepared by enrofloxacin powder into suspension with the concentration of 2 g/L.

1.3 Chicken colibacillosis model establishment and administration mode

A prevention test; the administration is carried out for 3d (the administration dose is shown in table 2) in advance according to an oral administration intragastric administration mode, then the toxicity counteracting and model building are carried out, except that 0.7mL of normal saline is injected into a blank control group, 0.7mL of bacterial liquid is injected into the left side and the right side of pectoralis muscles, sufficient drinking water and non-antibiotic additive feed are given, the administration is carried out for 5d after the toxicity counteracting and model building, 1 time every day, the observation is carried out for 2d after the medicine stopping, and then the dissection is carried out. 1-5 groups of chickens in the traditional Chinese medicine compound group are respectively administrated with the traditional Chinese medicine oral liquid medicines prepared in the examples 1-5; 6 to 10 groups of chickens in the traditional Chinese medicine compound group are respectively administrated with the traditional Chinese medicine oral liquid medicines prepared in the comparative examples 1 to 5; the chickens of the blank control group and the model control group are respectively irrigated with physiological saline with the same quantity. During this period, the chickens in each group were observed and tested for activity, mental status and death. The chicken in each group were examined for liver wrapping by dissection.

A treatment trial; the method for counteracting toxic substance and modeling comprises performing a prophylactic test, after 1 hr of counteracting toxic substance, infusing the corresponding dose of medicine 1 time per day for 5 days, stopping administration, observing for 2 days, and performing autopsy. The chickens in 1-5 groups of the traditional Chinese medicine compound groups are respectively administrated with the traditional Chinese medicine oral liquid medicines prepared in the examples 1-5; the chickens in 6-10 groups of the traditional Chinese medicine compound groups are respectively administrated with the traditional Chinese medicine oral liquid medicines prepared in the comparative examples 1-5; the chickens of the drug control group are administrated with 2g/L enrofloxacin suspension; the blank control group and the model control group were respectively infused with the same amount of physiological saline. During this period, the chickens in each group were observed and tested for activity, mental status and death. The condition that the chicken in each group wrapped the heart and wrapped the liver was examined by dissection.

1.4 evaluation of efficacy in the assay

Curing; during the test period, clinical symptoms basically disappeared after the administration, and the spirit and ingestion were recovered to normal.

The effect is displayed; during the test period, the clinical symptoms after the administration are obviously relieved, and the spirit and the ingestion are basically recovered.

The method is effective; during the trial, clinical symptoms were reduced after administration, but mental and feeding status was not substantially restored.

The total effective rate; after the test is finished, the sum of the cure number, the obvious effect number and the effective number of the chicken flocks accounts for the percentage of the treatment number of the chicken flocks.

Pericardial liver rate; after the experiment, the condition of the liver enveloped by the core (pericarditis) of each group of chicken (perihepatitis) is examined through anatomical examination (the liver enveloped by the core is shown in figure 2, and the liver enveloped by the healthy chicken is shown in figure 1). The following cases; the dead chicken, pericarditis and perihepatitis are judged to be wrapped heart and wrapped liver.

1.5 data analysis and processing

And (3) performing data analysis by adopting a sps 20 statistical processing software, and comparing the difference between the total effective rate and the core-wrapped liver rate of each group.

1.6 preventive test results

As can be seen from table 3, the prevention test results show that each toxicity attacking group has a pericardial liver covering phenomenon, except for the blank control group, the pericardial liver covering rate of the oral liquid group in example 1 is lower than that of the test groups in other examples and comparative examples, and partial anatomical pictures are shown in fig. 1 to 4; the total effective rate of the oral liquid group in the example 1 is obviously different from that of the model control group (P is less than 0.05). The traditional Chinese medicine compound provided by the invention can effectively prevent chicken colibacillosis.

TABLE 3 prevention and test of Total effective rate and liver-wrapping rate of each group

Note: the data in the same column marked with a different lower case letter indicates significant difference (P < 0.05), and the data in the same lower case letter indicates insignificant difference (P > 0.05).

1.7 therapeutic test results

As can be seen from table 4, the pericardial liver covering phenomenon exists in each toxicity attacking group, and as shown in fig. 1 to 4, except for the blank control group, the pericardial liver covering rate of the oral liquid group in example 1 is lower than that of the test groups in other examples and comparative examples; the total effective rate of the oral liquid group in the example 1 is obviously different from that of a model control group (P is less than 0.05), and is not obviously different from that of a drug control group (P is more than 0.05). The traditional Chinese medicine composition provided by the invention can effectively treat chicken colibacillosis.

TABLE 4 Total effective rate and liver-wrapping rate of each treatment group

Note: the data in the same column indicates significant difference (P < 0.05) when the lower case letters are different from the shoulder marks, and the data in the same lower case letters indicate insignificant difference (P > 0.05).

As proved by the tests, the Chinese herbal compound preparation in the embodiment 1 can effectively reduce the mortality of escherichia coli in both a prevention test and a treatment test, but the treatment effect is slightly lower than the prevention effect. The treatment effect of the traditional Chinese medicine compound in the embodiment 1 is not different from that of the drug control group (P is more than 0.05), and the effect of the traditional Chinese medicine compound in the embodiment 1 in a treatment test is slightly better than that of the drug control group.

Example of clinical test

28000 white feather broilers of 26 days old are raised in a certain farm in Taian province in Liaoning province, part of the sick chickens are depressed and are cachectic (as shown in figure 5), the chickens are almost refused to eat, and obvious wrapped hearts and liver are only seen in the dead chickens subjected to autopsy. The heart blood and liver disease material of the sick chicken is taken, the chicken grows well in the Mackanka after being processed, the surface of a bacterial colony is smooth and moist, the edge is neat, and then the bacterial colony is identified by using an eosin-methylene blue culture medium, is black or purple black, has metallic luster, and can be judged as chicken colibacillosis. 1187 sick chickens were found together, 231 were dead before dosing and every day. After the traditional Chinese medicine compound group is used, 161 die in the period, 74 die out with poor effect, 721 die with better effect and 75 percent of total effective rate. The traditional Chinese medicine compound test group 1 can effectively reduce the death rate of the chickens, reduce the generation of the symptom wrapping pericardial liver, is beneficial to improving the feed intake of the chickens, and enables the whole chicken group to be basically recovered to be normal. The results of the specific clinical trial examples are shown in Table 5 below.

TABLE 5 death and culling records of chickens during dosing period (only)

The traditional Chinese medicine composition oral liquid has the same effect as a sensitive antibiotic in clinical prevention and treatment of septicemia colibacillosis with drug resistance.

Finally, it should be noted that: the above examples are only intended to illustrate the technical solution of the present invention, but not to limit it; although the present invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical solutions described in the foregoing embodiments may still be modified, or some technical features may be equivalently replaced; and such modifications or substitutions do not depart from the spirit and scope of the corresponding technical solutions of the embodiments of the present invention.

Claims (10)

1. The traditional Chinese medicine composition for preventing and treating chicken colibacillosis is characterized in that active ingredients comprise the following raw materials in parts by weight: 20 to 40 portions of Japanese thistle herb, 10 to 30 portions of mulberry leaf, 10 to 30 portions of isatis root, 5 to 15 portions of largehead atractylodes rhizome, 5 to 15 portions of hawthorn and 5 to 10 portions of liquorice.

2. The traditional Chinese medicine composition according to claim 1, wherein the active ingredients comprise the following raw materials in parts by weight: 20 to 40 parts of Japanese thistle herb, 15 to 30 parts of mulberry leaf, 15 to 30 parts of isatis root, 5 to 15 parts of largehead atractylodes rhizome, 5 to 15 parts of hawthorn and 5 to 10 parts of liquorice;

optionally, the feed comprises the following raw materials in parts by weight: 30 to 40 parts of Japanese thistle herb, 20 to 30 parts of mulberry leaf, 20 to 30 parts of isatis root, 10 to 15 parts of largehead atractylodes rhizome, 10 to 15 parts of hawthorn and 8 to 10 parts of liquorice.

3. The traditional Chinese medicine composition according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 30 to 35 portions of Japanese thistle herb, 20 to 25 portions of mulberry leaf, 20 to 25 portions of isatis root, 10 to 15 portions of largehead atractylodes rhizome, 10 to 15 portions of hawthorn and 8 to 10 portions of liquorice.

4. The traditional Chinese medicine composition according to claim 1, which is characterized by comprising the following raw materials in parts by weight: 30 parts of Japanese thistle herb, 20 parts of mulberry leaf, 20 parts of isatis root, 10 parts of bighead atractylodes rhizome, 10 parts of hawthorn and 8 parts of liquorice.

5. The traditional Chinese medicine composition of claim 1, wherein the composition is in the form of any one of an injection, a powder, a capsule, a tablet and an oral liquid.

6. The traditional Chinese medicine composition as claimed in claim 5, wherein the dosage form of the composition is oral liquid;

optionally, the crude drug amount of the oral liquid is 0.5-2 g/mL, optionally 1g/mL;

optionally, the oral liquid is prepared by decocting and concentrating the Chinese medicinal composition.

7. A method for preparing the Chinese medicinal composition of any one of claims 1 to 6, which comprises the following steps: the medicine is prepared by taking the raw materials of the medicinal materials according to the parts by weight, crushing, decocting and concentrating.

8. The method of claim 7, comprising the steps of:

1) Taking the medicinal materials according to the weight parts, crushing and sieving; sieving with a second sieve;

2) Weighing the raw material medicine sieved in the step 1), adding water with the weight being 9-11 times of the total weight, soaking the raw material medicine for 0.5-1 hour before decocting, and decocting for 1-3 hours;

3) Filtering the decocted traditional Chinese medicine in the step 2), adding water 7-9 times of the total weight of the medicinal materials into filter residues, and decocting for 1-2 hours;

4) Filtering the decocted traditional Chinese medicine in the step 3), adding water which is 6-8 times of the total weight of the medicinal materials, and decocting for 1-2 hours;

5) Mixing the filtered liquid medicines in the steps 2), 3) and 4), concentrating the liquid medicine until the crude drug amount is 1g/mL, and sterilizing to prepare the oral liquid.

9. An application of the traditional Chinese medicine composition according to any one of claims 1 to 6 or the traditional Chinese medicine composition prepared by the preparation method according to any one of claims 7 to 8 in preparation of a medicine for preventing or treating chicken colibacillosis.

10. The use of claim 9, wherein the chicken colibacillosis comprises one or more of colibacillary pericarditis, perihepatitis, peritonitis, salpingitis, umbilicitis, synovitis, air sacculitis, granuloma and ophthalmia, optionally chicken septicemia colibacillosis.

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