CN115772512A - 腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用 - Google Patents

腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用 Download PDF

Info

Publication number
CN115772512A
CN115772512A CN202111044206.0A CN202111044206A CN115772512A CN 115772512 A CN115772512 A CN 115772512A CN 202111044206 A CN202111044206 A CN 202111044206A CN 115772512 A CN115772512 A CN 115772512A
Authority
CN
China
Prior art keywords
adenine
amino acid
deaminase
mutated
acid residue
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Pending
Application number
CN202111044206.0A
Other languages
English (en)
Chinese (zh)
Inventor
陈亮
李大力
张舜
薛念念
刘明耀
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
East China Normal University
Bioray Laboratories Inc
Original Assignee
East China Normal University
Bioray Laboratories Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by East China Normal University, Bioray Laboratories Inc filed Critical East China Normal University
Priority to CN202111044206.0A priority Critical patent/CN115772512A/zh
Priority to PCT/CN2022/117592 priority patent/WO2023036189A1/fr
Publication of CN115772512A publication Critical patent/CN115772512A/zh
Pending legal-status Critical Current

Links

Images

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/78—Hydrolases (3) acting on carbon to nitrogen bonds other than peptide bonds (3.5)

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
CN202111044206.0A 2021-09-07 2021-09-07 腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用 Pending CN115772512A (zh)

Priority Applications (2)

Application Number Priority Date Filing Date Title
CN202111044206.0A CN115772512A (zh) 2021-09-07 2021-09-07 腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用
PCT/CN2022/117592 WO2023036189A1 (fr) 2021-09-07 2022-09-07 Adénine déaminase, éditeur de base adénine la contenant, et ses applications

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202111044206.0A CN115772512A (zh) 2021-09-07 2021-09-07 腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用

Publications (1)

Publication Number Publication Date
CN115772512A true CN115772512A (zh) 2023-03-10

Family

ID=85387684

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202111044206.0A Pending CN115772512A (zh) 2021-09-07 2021-09-07 腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用

Country Status (2)

Country Link
CN (1) CN115772512A (fr)
WO (1) WO2023036189A1 (fr)

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2019005884A1 (fr) * 2017-06-26 2019-01-03 The Broad Institute, Inc. Compositions à base de crispr/cas-adénine désaminase, systèmes et procédés d'édition ciblée d'acides nucléiques
CA3129158A1 (fr) * 2019-02-13 2020-08-20 Beam Therapeutics Inc. Editeurs de base adenosine desaminase et leurs methodes d'utilisation pour modifier une nucleobase dans une sequence cible
CN110407945A (zh) * 2019-06-14 2019-11-05 上海科技大学 一种腺嘌呤碱基编辑工具及其用途
KR20220110739A (ko) * 2019-10-30 2022-08-09 페어와이즈 플랜츠 서비시즈, 인크. 유형 v crispr-cas 염기 편집제 및 그의 사용 방법
WO2021158921A2 (fr) * 2020-02-05 2021-08-12 The Broad Institute, Inc. Éditeurs de base d'adénine et leurs utilisations
CN112143753A (zh) * 2020-09-17 2020-12-29 中国农业科学院植物保护研究所 一套腺嘌呤碱基编辑器及其相关生物材料与应用
CN115247162B (zh) * 2021-04-27 2024-05-03 华东师范大学 一种腺嘌呤碱基编辑用融合蛋白及其应用

Also Published As

Publication number Publication date
WO2023036189A1 (fr) 2023-03-16

Similar Documents

Publication Publication Date Title
CN109957569B (zh) 基于cpf1蛋白的碱基编辑系统和方法
KR102484553B1 (ko) A형 혈우병의 유전자 요법을 위한 증가된 발현을 갖는 재조합 fviii 변이체를 인코딩하는 바이러스 벡터
CN110157727A (zh) 植物碱基编辑方法
CN110551761B (zh) CRISPR/Sa-SepCas9基因编辑系统及其应用
CN112159801B (zh) SlugCas9-HF蛋白、含有SlugCas9-HF蛋白的基因编辑系统及应用
CN109517845A (zh) 一种crispr单碱基修复系统及其应用
WO2023193536A1 (fr) Adénosine désaminase, éditeur de bases et leur utilisation
CN110577971B (zh) CRISPR/Sa-SauriCas9基因编辑系统及其应用
CN116410963A (zh) 一种实现高效碱基颠换的迷你基因编辑系统及其应用
CN110499335B (zh) CRISPR/SauriCas9基因编辑系统及其应用
CN115772512A (zh) 腺嘌呤脱氨酶、包含其的腺嘌呤碱基编辑器及其应用
CN110577969B (zh) CRISPR/Sa-SlugCas9基因编辑系统及其应用
CN110551762A (zh) CRISPR/ShaCas9基因编辑系统及其应用
CN113564145A (zh) 用于胞嘧啶碱基编辑的融合蛋白及其应用
CN110577970B (zh) CRISPR/Sa-SlutCas9基因编辑系统及其应用
CN112979823B (zh) 一种用于治疗和/或预防β血红蛋白病的产品及融合蛋白
CN110577972B (zh) CRISPR/Sa-ShaCas9基因编辑系统及其应用
CN110551763B (zh) CRISPR/SlutCas9基因编辑系统及其应用
WO2023125814A1 (fr) Adénine désaminase et son application
WO2021133261A1 (fr) Éditeurs de nucléobase
CN116410964A (zh) 腺嘌呤脱氨酶及其应用
CN110551760A (zh) 一种CRISPR/Sa-SeqCas9基因编辑系统和其应用
WO2023024089A1 (fr) Système d'édition de bases permettant d'obtenir une mutation de base de a à c et/ou de a à t et son utilisation
US20240158793A1 (en) Repressor proteins for gene regulation and crispr interference
US20240052370A1 (en) Modulating cellular repair mechanisms for genomic editing

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination