CN115698297A - 多模块生物合成酶基因组合文库的制备方法 - Google Patents

多模块生物合成酶基因组合文库的制备方法 Download PDF

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CN115698297A
CN115698297A CN202180037639.5A CN202180037639A CN115698297A CN 115698297 A CN115698297 A CN 115698297A CN 202180037639 A CN202180037639 A CN 202180037639A CN 115698297 A CN115698297 A CN 115698297A
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dna
dna fragments
gene cluster
plasmid
gene
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大卫·利普斯
海斯·韦尔卡雷吉
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Spiber Inc
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Spiber Inc
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    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/76—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Actinomyces; for Streptomyces
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    • C12N9/10—Transferases (2.)
    • C12N9/1025—Acyltransferases (2.3)
    • C12N9/1029—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1068—Template (nucleic acid) mediated chemical library synthesis, e.g. chemical and enzymatical DNA-templated organic molecule synthesis, libraries prepared by non ribosomal polypeptide synthesis [NRPS], DNA/RNA-polymerase mediated polypeptide synthesis
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease
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    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
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    • C12Y203/00—Acyltransferases (2.3)
    • C12Y203/01—Acyltransferases (2.3) transferring groups other than amino-acyl groups (2.3.1)
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
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    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B50/00—Methods of creating libraries, e.g. combinatorial synthesis
    • C40B50/06—Biochemical methods, e.g. using enzymes or whole viable microorganisms
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12R—INDEXING SCHEME ASSOCIATED WITH SUBCLASSES C12C - C12Q, RELATING TO MICROORGANISMS
    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/07—Bacillus
    • C12R2001/125—Bacillus subtilis ; Hay bacillus; Grass bacillus
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
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    • C12R2001/00—Microorganisms ; Processes using microorganisms
    • C12R2001/01—Bacteria or Actinomycetales ; using bacteria or Actinomycetales
    • C12R2001/465—Streptomyces
    • C12R2001/485—Streptomyces aureofaciens

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  • Bioinformatics & Cheminformatics (AREA)
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  • Medicinal Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
CN202180037639.5A 2020-05-26 2021-05-25 多模块生物合成酶基因组合文库的制备方法 Pending CN115698297A (zh)

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JP2020091799 2020-05-26
JP2020-091799 2020-05-26
PCT/JP2021/019849 WO2021241593A1 (ja) 2020-05-26 2021-05-25 マルチモジュール型生合成酵素遺伝子のコンビナトリアルライブラリーの調製方法

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CN115698297A true CN115698297A (zh) 2023-02-03

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US (1) US20230174956A1 (https=)
EP (1) EP4159862A4 (https=)
JP (1) JPWO2021241593A1 (https=)
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Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN115667519A (zh) * 2020-05-26 2023-01-31 丝芭博株式会社 含i型聚酮合酶基因的质粒的制备方法

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Publication number Priority date Publication date Assignee Title
WO2022250068A1 (ja) * 2021-05-25 2022-12-01 Ｓｐｉｂｅｒ株式会社 プラスミドの製造方法及びプラスミド
EP4563701A1 (en) * 2022-07-29 2025-06-04 National University Corporation Kobe University Peptide synthetase library construction method
JP2026009432A (ja) * 2022-09-20 2026-01-21 Ｓｐｉｂｅｒ株式会社 Ｄｎａ配列のスクリーニング方法
CN121358846A (zh) * 2023-06-09 2026-01-16 新博基股份有限公司 菌株的物质生产

Citations (3)

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JP2004129654A (ja) * 2002-09-19 2004-04-30 Mitsubishi Chemicals Corp 挿入ｄｎａユニットを含むプラスミドの製造方法
CN106170547A (zh) * 2014-01-21 2016-11-30 高效基因设计技术研究协会 单元dna组合物的制备方法及dna连结体的制作方法
CN115667519A (zh) * 2020-05-26 2023-01-31 丝芭博株式会社 含i型聚酮合酶基因的质粒的制备方法

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US6753173B1 (en) * 1999-02-09 2004-06-22 Board Of Trustees Of The Leland Stanford Junior University Methods to mediate polyketide synthase module effectiveness
US7427493B2 (en) * 2002-06-28 2008-09-23 Kosan Biosciences Incorporated Recombinant genes for polyketide modifying enzymes
JP2006517090A (ja) * 2002-09-26 2006-07-20 コーサン バイオサイエンシーズ， インコーポレイテッド 合成遺伝子
CA2541177A1 (en) * 2003-10-03 2005-09-22 Promega Corporation Vectors for directional cloning
US20050227316A1 (en) * 2004-04-07 2005-10-13 Kosan Biosciences, Inc. Synthetic genes
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AU2009214435C1 (en) 2008-02-15 2014-07-17 Synthetic Genomics, Inc. Methods for in vitro joining and combinatorial assembly of nucleic acid molecules
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CN110129244B (zh) * 2019-01-23 2023-09-15 河北圣雪大成制药有限责任公司 链霉菌底盘菌株及其构建方法、在异源表达研究中的应用

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JP2004129654A (ja) * 2002-09-19 2004-04-30 Mitsubishi Chemicals Corp 挿入ｄｎａユニットを含むプラスミドの製造方法
CN106170547A (zh) * 2014-01-21 2016-11-30 高效基因设计技术研究协会 单元dna组合物的制备方法及dna连结体的制作方法
CN115667519A (zh) * 2020-05-26 2023-01-31 丝芭博株式会社 含i型聚酮合酶基因的质粒的制备方法

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JANA KUMPFMÜLLER等: "Production of the polyketide 6-deoxyerythronolide B in the heterologous host Bacillus subtilis", APPL MICROBIOL BIOTECHNOL, vol. 100, 2 October 2015 (2015-10-02), pages 1214 *

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Publication number Priority date Publication date Assignee Title
CN115667519A (zh) * 2020-05-26 2023-01-31 丝芭博株式会社 含i型聚酮合酶基因的质粒的制备方法

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EP4159862A4 (en) 2024-06-05
WO2021241593A1 (ja) 2021-12-02
EP4159862A1 (en) 2023-04-05
US20230174956A1 (en) 2023-06-08

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