CN115698280A - 新的丝氨酸蛋白酶变体 - Google Patents

新的丝氨酸蛋白酶变体 Download PDF

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Publication number
CN115698280A
CN115698280A CN202280004542.9A CN202280004542A CN115698280A CN 115698280 A CN115698280 A CN 115698280A CN 202280004542 A CN202280004542 A CN 202280004542A CN 115698280 A CN115698280 A CN 115698280A
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Chinese (zh)
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李周姬
曹阿拉
池昶俊
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CJ CheilJedang Corp
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CJ CheilJedang Corp
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    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K20/00—Accessory food factors for animal feeding-stuffs
    • A23K20/10—Organic substances
    • A23K20/189—Enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/16—Addition of microorganisms or extracts thereof, e.g. single-cell proteins, to feeding-stuff compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/75—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Bacillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y304/00—Hydrolases acting on peptide bonds, i.e. peptidases (3.4)
    • C12Y304/21—Serine endopeptidases (3.4.21)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/02—Libraries contained in or displayed by microorganisms, e.g. bacteria or animal cells; Libraries contained in or displayed by vectors, e.g. plasmids; Libraries containing only microorganisms or vectors
    • C—CHEMISTRY; METALLURGY
    • C40—COMBINATORIAL TECHNOLOGY
    • C40B—COMBINATORIAL CHEMISTRY; LIBRARIES, e.g. CHEMICAL LIBRARIES
    • C40B40/00—Libraries per se, e.g. arrays, mixtures
    • C40B40/04—Libraries containing only organic compounds
    • C40B40/06—Libraries containing nucleotides or polynucleotides, or derivatives thereof
    • C40B40/08—Libraries containing RNA or DNA which encodes proteins, e.g. gene libraries

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Polymers & Plastics (AREA)
  • Plant Pathology (AREA)
  • Animal Husbandry (AREA)
  • Food Science & Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Detergent Compositions (AREA)
CN202280004542.9A 2021-03-12 2022-03-11 新的丝氨酸蛋白酶变体 Pending CN115698280A (zh)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR1020210032885A KR102613549B1 (ko) 2021-03-12 2021-03-12 신규 세린 프로테아제 변이체
KR10-2021-0032885 2021-03-12
PCT/KR2022/003415 WO2022191653A1 (ko) 2021-03-12 2022-03-11 신규 세린 프로테아제 변이체

Publications (1)

Publication Number Publication Date
CN115698280A true CN115698280A (zh) 2023-02-03

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CN202280004542.9A Pending CN115698280A (zh) 2021-03-12 2022-03-11 新的丝氨酸蛋白酶变体

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Country Link
US (1) US12570967B2 (https=)
EP (1) EP4116416A4 (https=)
JP (2) JP7676439B2 (https=)
KR (1) KR102613549B1 (https=)
CN (1) CN115698280A (https=)
CA (1) CA3175414A1 (https=)
TW (1) TWI887529B (https=)
WO (1) WO2022191653A1 (https=)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN116904428B (zh) * 2023-09-11 2024-01-02 中国农业科学院北京畜牧兽医研究所 一种改良高温碱性蛋白酶AprThc的稳定性的方法及突变体
KR20250054900A (ko) * 2023-10-16 2025-04-24 씨제이제일제당 (주) 신규 세린 프로테아제 변이체

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1198165A (zh) * 1995-07-16 1998-11-04 耶达研究与发展有限公司 Fas受体和其它蛋白质功能的调节物
CN101842480A (zh) * 2007-10-30 2010-09-22 丹尼斯科美国公司 链霉菌蛋白酶
CN110381746A (zh) * 2016-12-21 2019-10-25 杜邦营养生物科学有限公司 使用热稳定丝氨酸蛋白酶的方法

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AR016969A1 (es) 1997-10-23 2001-08-01 Procter & Gamble VARIANTE DE PROTEASA, ADN, VECTOR DE EXPRESIoN, MICROORGANISMO HUESPED, COMPOSICIoN DE LIMPIEZA, ALIMENTO PARA ANIMALES Y COMPOSICIoN PARA TRATAR UN TEXTIL
US7985569B2 (en) 2003-11-19 2011-07-26 Danisco Us Inc. Cellulomonas 69B4 serine protease variants
WO2005052146A2 (en) 2003-11-19 2005-06-09 Genencor International, Inc. Serine proteases, nucleic acids encoding serine enzymes and vectors and host cells incorporating same
KR100534660B1 (ko) 2003-12-30 2005-12-08 주식회사 해찬들 단백질 분해효소 생성량이 증가된 메주의 제조방법 및 된장
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
CN103667323B (zh) 2007-03-12 2016-02-03 丹尼斯科美国公司 经修饰的蛋白酶
CN102209778B (zh) 2008-11-11 2014-10-15 丹尼斯科美国公司 包含丝氨酸蛋白酶变体的组合物和方法
BR112014017919A2 (pt) * 2012-01-26 2017-06-27 Novozymes As uso de um poliptídeo variante, composição, polinucleotídeo isolado, constructo de ácido nucleico ou vetor de expressão, célula hospedeira de expressão recombinante, métodos de produção do pilipeptídeo, para melhoria do valor nutricional de uma ração animal, e para o tratamento de proteínas, planta, parte de planta ou célula de planta transgênica, aditivo de ração animal, ração animal, e, composição de ração animal ou detergente
AU2014214032A1 (en) * 2013-02-06 2015-07-09 Novozymes A/S Use of polypeptides having protease activity in animal feed
WO2014209789A1 (en) 2013-06-24 2014-12-31 Novozymes A/S Process of extracting oil from thin stillage
EP3227456A1 (en) * 2014-12-01 2017-10-11 Novozymes A/S Method for producing a protein hydrolysate
KR101632642B1 (ko) 2015-01-29 2016-06-22 씨제이제일제당 주식회사 신규한 프로모터 및 그의 용도
CN108350444A (zh) 2015-09-25 2018-07-31 诺维信公司 丝氨酸蛋白酶用于改进乙醇产量的用途
CA3028875A1 (en) 2016-07-21 2018-01-25 Novozymes A/S Serine protease variants and polynucleotides encoding same
MY186296A (en) 2016-08-31 2021-07-06 Cj Cheiljedang Corp Novel promoter and use thereof
KR20190086540A (ko) 2016-12-01 2019-07-22 바스프 에스이 조성물 중 효소의 안정화
KR102316445B1 (ko) 2019-11-29 2021-10-26 씨제이제일제당 주식회사 신규 세린 프로테아제 변이체

Patent Citations (3)

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Publication number Priority date Publication date Assignee Title
CN1198165A (zh) * 1995-07-16 1998-11-04 耶达研究与发展有限公司 Fas受体和其它蛋白质功能的调节物
CN101842480A (zh) * 2007-10-30 2010-09-22 丹尼斯科美国公司 链霉菌蛋白酶
CN110381746A (zh) * 2016-12-21 2019-10-25 杜邦营养生物科学有限公司 使用热稳定丝氨酸蛋白酶的方法

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US20230174964A1 (en) 2023-06-08
WO2022191653A1 (ko) 2022-09-15
JP2023521203A (ja) 2023-05-23
JP7676439B2 (ja) 2025-05-14
US12570967B2 (en) 2026-03-10
EP4116416A1 (en) 2023-01-11
CA3175414A1 (en) 2022-09-15
KR20220128171A (ko) 2022-09-20
KR102613549B1 (ko) 2023-12-13
JP2025011087A (ja) 2025-01-23
TWI887529B (zh) 2025-06-21
TW202237835A (zh) 2022-10-01
EP4116416A4 (en) 2023-10-11

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