CN115606704B - Hair bulb remover for improving gastrointestinal health of pets and preparation method thereof - Google Patents
Hair bulb remover for improving gastrointestinal health of pets and preparation method thereof Download PDFInfo
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- CN115606704B CN115606704B CN202211359205.XA CN202211359205A CN115606704B CN 115606704 B CN115606704 B CN 115606704B CN 202211359205 A CN202211359205 A CN 202211359205A CN 115606704 B CN115606704 B CN 115606704B
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- 210000004209 hair Anatomy 0.000 title claims abstract description 89
- 230000002496 gastric effect Effects 0.000 title claims abstract description 24
- 238000002360 preparation method Methods 0.000 title claims abstract description 18
- 244000063299 Bacillus subtilis Species 0.000 claims abstract description 62
- 235000014469 Bacillus subtilis Nutrition 0.000 claims abstract description 62
- 238000000855 fermentation Methods 0.000 claims abstract description 55
- 230000004151 fermentation Effects 0.000 claims abstract description 55
- 241000282326 Felis catus Species 0.000 claims abstract description 28
- 239000003381 stabilizer Substances 0.000 claims abstract description 20
- 230000002195 synergetic effect Effects 0.000 claims abstract description 18
- XOAAWQZATWQOTB-UHFFFAOYSA-N taurine Chemical compound NCCS(O)(=O)=O XOAAWQZATWQOTB-UHFFFAOYSA-N 0.000 claims description 18
- FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 claims description 14
- 239000002609 medium Substances 0.000 claims description 12
- 239000000203 mixture Substances 0.000 claims description 12
- 238000000034 method Methods 0.000 claims description 11
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 claims description 10
- 239000004227 calcium gluconate Substances 0.000 claims description 9
- 229960004494 calcium gluconate Drugs 0.000 claims description 9
- 235000013927 calcium gluconate Nutrition 0.000 claims description 9
- MKJXYGKVIBWPFZ-UHFFFAOYSA-L calcium lactate Chemical compound [Ca+2].CC(O)C([O-])=O.CC(O)C([O-])=O MKJXYGKVIBWPFZ-UHFFFAOYSA-L 0.000 claims description 9
- 229960002401 calcium lactate Drugs 0.000 claims description 9
- 239000001527 calcium lactate Substances 0.000 claims description 9
- 235000011086 calcium lactate Nutrition 0.000 claims description 9
- NEEHYRZPVYRGPP-UHFFFAOYSA-L calcium;2,3,4,5,6-pentahydroxyhexanoate Chemical compound [Ca+2].OCC(O)C(O)C(O)C(O)C([O-])=O.OCC(O)C(O)C(O)C(O)C([O-])=O NEEHYRZPVYRGPP-UHFFFAOYSA-L 0.000 claims description 9
- 239000001726 jatropha manihot extract Substances 0.000 claims description 9
- 229960003080 taurine Drugs 0.000 claims description 9
- 229940106668 yucca extract Drugs 0.000 claims description 9
- VTYYLEPIZMXCLO-UHFFFAOYSA-L Calcium carbonate Chemical compound [Ca+2].[O-]C([O-])=O VTYYLEPIZMXCLO-UHFFFAOYSA-L 0.000 claims description 8
- YYRMJZQKEFZXMX-UHFFFAOYSA-L calcium bis(dihydrogenphosphate) Chemical compound [Ca+2].OP(O)([O-])=O.OP(O)([O-])=O YYRMJZQKEFZXMX-UHFFFAOYSA-L 0.000 claims description 7
- 238000012258 culturing Methods 0.000 claims description 7
- 235000019691 monocalcium phosphate Nutrition 0.000 claims description 7
- 239000011780 sodium chloride Substances 0.000 claims description 7
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 7
- 210000001035 gastrointestinal tract Anatomy 0.000 claims description 6
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 claims description 5
- 240000007594 Oryza sativa Species 0.000 claims description 5
- 235000007164 Oryza sativa Nutrition 0.000 claims description 5
- 229930003268 Vitamin C Natural products 0.000 claims description 5
- 229910000389 calcium phosphate Inorganic materials 0.000 claims description 5
- 238000002156 mixing Methods 0.000 claims description 5
- 239000006916 nutrient agar Substances 0.000 claims description 5
- 235000009566 rice Nutrition 0.000 claims description 5
- 235000019154 vitamin C Nutrition 0.000 claims description 5
- 239000011718 vitamin C Substances 0.000 claims description 5
- 229910000019 calcium carbonate Inorganic materials 0.000 claims description 4
- 239000001963 growth medium Substances 0.000 claims description 4
- 230000001954 sterilising effect Effects 0.000 claims description 3
- 235000010216 calcium carbonate Nutrition 0.000 claims description 2
- 229940062672 calcium dihydrogen phosphate Drugs 0.000 claims description 2
- 238000000643 oven drying Methods 0.000 claims description 2
- 238000010298 pulverizing process Methods 0.000 claims description 2
- 238000007873 sieving Methods 0.000 claims description 2
- 239000002131 composite material Substances 0.000 claims 1
- 239000003085 diluting agent Substances 0.000 claims 1
- 241000282472 Canis lupus familiaris Species 0.000 abstract description 20
- 102000011782 Keratins Human genes 0.000 abstract description 13
- 108010076876 Keratins Proteins 0.000 abstract description 13
- 230000001737 promoting effect Effects 0.000 abstract 1
- 108010059345 keratinase Proteins 0.000 description 24
- 230000000694 effects Effects 0.000 description 21
- 239000002253 acid Substances 0.000 description 13
- 230000000052 comparative effect Effects 0.000 description 11
- 210000000936 intestine Anatomy 0.000 description 10
- 210000002784 stomach Anatomy 0.000 description 10
- VEXZGXHMUGYJMC-UHFFFAOYSA-N Hydrochloric acid Chemical compound Cl VEXZGXHMUGYJMC-UHFFFAOYSA-N 0.000 description 6
- 230000000593 degrading effect Effects 0.000 description 6
- 230000008673 vomiting Effects 0.000 description 6
- 206010004542 Bezoar Diseases 0.000 description 4
- 230000002378 acidificating effect Effects 0.000 description 4
- 230000029087 digestion Effects 0.000 description 4
- 239000002480 mineral oil Substances 0.000 description 4
- 235000010446 mineral oil Nutrition 0.000 description 4
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 4
- 235000019796 monopotassium phosphate Nutrition 0.000 description 4
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 4
- 206010047700 Vomiting Diseases 0.000 description 3
- 230000029142 excretion Effects 0.000 description 3
- 239000000758 substrate Substances 0.000 description 3
- 210000004916 vomit Anatomy 0.000 description 3
- AFCARXCZXQIEQB-UHFFFAOYSA-N N-[3-oxo-3-(2,4,6,7-tetrahydrotriazolo[4,5-c]pyridin-5-yl)propyl]-2-[[3-(trifluoromethoxy)phenyl]methylamino]pyrimidine-5-carboxamide Chemical compound O=C(CCNC(=O)C=1C=NC(=NC=1)NCC1=CC(=CC=C1)OC(F)(F)F)N1CC2=C(CC1)NN=N2 AFCARXCZXQIEQB-UHFFFAOYSA-N 0.000 description 2
- 238000010521 absorption reaction Methods 0.000 description 2
- 239000001506 calcium phosphate Substances 0.000 description 2
- HVYWMOMLDIMFJA-DPAQBDIFSA-N cholesterol Chemical compound C1C=C2C[C@@H](O)CC[C@]2(C)[C@@H]2[C@@H]1[C@@H]1CC[C@H]([C@H](C)CCCC(C)C)[C@@]1(C)CC2 HVYWMOMLDIMFJA-DPAQBDIFSA-N 0.000 description 2
- 238000010790 dilution Methods 0.000 description 2
- 239000012895 dilution Substances 0.000 description 2
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000008172 hydrogenated vegetable oil Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 229910000150 monocalcium phosphate Inorganic materials 0.000 description 2
- 208000024891 symptom Diseases 0.000 description 2
- 235000015112 vegetable and seed oil Nutrition 0.000 description 2
- 239000008158 vegetable oil Substances 0.000 description 2
- 206010010774 Constipation Diseases 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 208000008589 Obesity Diseases 0.000 description 1
- 108091005804 Peptidases Proteins 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- 239000004365 Protease Substances 0.000 description 1
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 1
- 208000020560 abdominal swelling Diseases 0.000 description 1
- 230000004594 appetite change Effects 0.000 description 1
- 235000015278 beef Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- GEHJBWKLJVFKPS-UHFFFAOYSA-N bromochloroacetic acid Chemical compound OC(=O)C(Cl)Br GEHJBWKLJVFKPS-UHFFFAOYSA-N 0.000 description 1
- 230000015556 catabolic process Effects 0.000 description 1
- 235000012000 cholesterol Nutrition 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000006731 degradation reaction Methods 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 239000012153 distilled water Substances 0.000 description 1
- 230000002708 enhancing effect Effects 0.000 description 1
- 238000001952 enzyme assay Methods 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000008187 granular material Substances 0.000 description 1
- 208000019622 heart disease Diseases 0.000 description 1
- 230000002779 inactivation Effects 0.000 description 1
- 230000000968 intestinal effect Effects 0.000 description 1
- 230000007794 irritation Effects 0.000 description 1
- 235000021266 loss of appetite Nutrition 0.000 description 1
- 230000001050 lubricating effect Effects 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020824 obesity Nutrition 0.000 description 1
- 239000003921 oil Substances 0.000 description 1
- 235000019198 oils Nutrition 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940045997 vitamin a Drugs 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K50/00—Feeding-stuffs specially adapted for particular animals
- A23K50/40—Feeding-stuffs specially adapted for particular animals for carnivorous animals, e.g. cats or dogs
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
- A23K10/12—Animal feeding-stuffs obtained by microbiological or biochemical processes by fermentation of natural products, e.g. of vegetable material, animal waste material or biomass
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K10/00—Animal feeding-stuffs
- A23K10/30—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms
- A23K10/37—Animal feeding-stuffs from material of plant origin, e.g. roots, seeds or hay; from material of fungal origin, e.g. mushrooms from waste material
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/105—Aliphatic or alicyclic compounds
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/142—Amino acids; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/10—Organic substances
- A23K20/174—Vitamins
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K20/00—Accessory food factors for animal feeding-stuffs
- A23K20/20—Inorganic substances, e.g. oligoelements
- A23K20/26—Compounds containing phosphorus
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23K—FODDER
- A23K40/00—Shaping or working-up of animal feeding-stuffs
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- Chemical & Material Sciences (AREA)
- Polymers & Plastics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Food Science & Technology (AREA)
- Animal Husbandry (AREA)
- Biotechnology (AREA)
- Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Physiology (AREA)
- Biomedical Technology (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Birds (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Inorganic Chemistry (AREA)
- Botany (AREA)
- Mycology (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Fodder In General (AREA)
Abstract
The application discloses a hair bulb remover for improving gastrointestinal health of pets and a preparation method thereof, wherein the hair bulb remover for improving gastrointestinal health of pets comprises the following components in percentage by mass: 10-20% of bacillus subtilis fermentation product, 3-5% of synergistic accelerator, 3-6% of stabilizer and 69-85% of carrier. The hair bulb remover prepared by the application can effectively degrade keratin in hair in the bodies of cats and dogs, thereby promoting hair to be discharged out of the bodies of the pets.
Description
Technical Field
The application relates to the field of pet health, in particular to a hair bulb remover for improving gastrointestinal health of pets and a preparation method thereof.
Background
When the cat and dog pets comb own hairs at ordinary times, the hairs are easy to eat into intestines and stomach, especially the cat and dog pets comb own hairs with the tongue for a long time, and the hairs falling off from the body enter the body at the same time. Some hair can be discharged out of the body through vomiting or excretion, but some hair can be clustered and accumulated in the body, and because the body of a pet does not have enzymes for digesting hair, the clustered hair bulbs are difficult to discharge out of the body, and the pet is easy to suffer from symptoms of hair bulbs, such as frequent vomiting, constipation, even abdominal swelling, no appetite and the like.
In the market, the purpose of lubricating intestinal tracts is achieved mainly by feeding mineral oil or hydrogenated vegetable oil and the like to the pets, so that the pets can be helped to discharge the hair balls out of the body. However, these oils are easy to cause intestinal irritation of pets, and long-term intake of mineral oil can hinder the ability of pets to absorb fat-soluble vitamins, resulting in deficiency of vitamin a, and long-term intake of hydrogenated vegetable oil can easily cause obesity, high cholesterol and even heart disease of pets.
The keratin has stable structure and extremely strong hydrophobicity, and animal endogenous protease is difficult to effectively degrade. The characteristic that keratinase can specifically degrade keratin attracts attention of a plurality of researchers, but the most suitable pH value of keratinase is 7.5-10, and the effect of degrading keratin cannot be exerted in the gastrointestinal environment of dogs and cats.
Disclosure of Invention
In order to provide a product capable of effectively degrading keratin in a cat and dog pet, the application provides a hair bulb remover for improving gastrointestinal health of the pet and a preparation method thereof.
A hair bulb remover for improving gastrointestinal health of pets, comprising the following components in percentage by mass: 10-20% of bacillus subtilis fermentation product, 2-5% of synergistic accelerator, 3-6% of stabilizer and 75-85% of carrier.
According to the hair bulb remover disclosed by the application, the bacillus subtilis fermentation product is innovatively added, and the bacillus subtilis fermentation product has higher keratinase activity and better acid resistance, so that the hair bulb remover can keep better stability in intestines and stomach of dogs and cats, can be specifically combined with keratin in hair, plays a role in degrading keratin in hair bulbs, can help to improve intestines and stomach health of dogs and promote digestion and absorption. The synergistic accelerator can promote the bacillus subtilis fermentation product to play a role in degrading keratin in the hair bulb, the stabilizer can help to maintain the activity stability of the bacillus subtilis fermentation product in the gastrointestinal tract of dogs and cats, and the bacillus subtilis fermentation product, the synergistic accelerator and the stabilizer are matched to promote the hair bulb in the intestines and stomach of dogs to be discharged in a digestion and excretion mode, so that the condition that the dogs and cats vomit to discharge the hair bulb is reduced, and the occurrence of pet hair bulb can be effectively prevented.
The hair bulb remover consists of a bacillus subtilis fermentation product, a synergist, a stabilizer and a carrier, does not contain mineral oil or vegetable oil components, and is not easy to stimulate intestines and stomach of pets.
Preferably, the preparation method of the bacillus subtilis fermentation product comprises the following steps:
acid-resistant bacillus subtilis culture: inoculating bacillus subtilis into nutrient agar culture medium, shake culturing at 36.5-37.5deg.C for 24-48 hr, collecting 10-20% dilution of bacillus subtilis, adjusting pH to 2.5-3.5, and standing culturing at 36.5-37.5deg.C for 36-48 hr to obtain acid-resistant bacillus subtilis.
Preparation of bacillus subtilis fermentation products: adding mixture of cat hair and dog hair, calcium gluconate and Yucca extract into fermentation medium, sterilizing, inoculating Bacillus subtilis, culturing at 40-50deg.C for fermentation, oven drying the fermentation product, pulverizing, sieving to obtain Bacillus subtilis fermentation product, and refrigerating.
In the preparation process of the bacillus subtilis fermentation product, the bacillus subtilis is firstly cultured in an acidic environment with a low pH value to obtain the bacillus subtilis with high acid resistance, then the bacillus subtilis with high acid resistance is used as a strain, calcium gluconate and yucca extract are added into a culture medium, so that the keratinase is not easy to degrade and inactivate under the acidic condition, the acid resistance of the keratinase is enhanced, the yield of the keratinase is improved, and the inactivation rate of the bacillus subtilis fermentation product under the acidic condition is obviously reduced.
Preferably, sodium chloride 0.04-0.06%, calcium gluconate 0.02-0.04%, yucca extract 0.01-0.03%, and mixture of cat hair and dog hair 1-2% are added into 1000ml water, and pH is adjusted to 5.5-6.5.
Under the environment, the keratinase generated by the fermentation of the bacillus subtilis has higher activity of decomposing the keratinase in the hair, and meanwhile, the keratinase has better acid resistance, so that the pet hair can be effectively degraded, and the pet hair accumulated in the stomach and intestine can be degraded and excreted out of the body.
Preferably, in the preparation step of the bacillus subtilis fermentation product, the fermentation culture time is 72-96 hours.
Preferably, the synergistic accelerator is one or two of vitamin C and taurine.
Preferably, the synergistic accelerator is taurine with the mass percentage of at least 3 percent.
The activity of keratinase in the fermentation product of bacillus subtilis is improved to a certain extent by adding vitamin C and taurine, and the degradation effect of pet hair is improved, wherein the activity of keratinase is improved to a larger extent by independently adding taurine with the mass percent of at least 3%, which indicates that the activity effect of keratinase is obvious by improving the activity of keratinase by taurine.
Preferably, the stabilizer is one or more of calcium lactate, calcium biphosphate and calcium carbonate.
Preferably, the stabilizer is a combination of calcium dihydrogen phosphate and calcium lactate according to a mass ratio of 2-3:1.
The calcium lactate, the monocalcium phosphate and the calcium carbonate can enhance the stability of the keratinase, and after the monocalcium phosphate and the calcium lactate are matched in a certain proportion, the activity of the keratinase in a stronger acid environment is improved, and the keratinase can play a better role in an acid condition.
Preferably, the carrier is defatted rice bran.
The defatted rice bran, the bacillus subtilis fermentation product, the synergistic accelerator and the stabilizer have good uniform dispersibility, and the defatted rice bran can be used as a carrier to enable the bacillus subtilis fermentation product to contact with pet hair, so that the effect of degrading keratin in the pet hair by the bacillus subtilis fermentation product is promoted.
A preparation method of hair bulb remover for improving gastrointestinal health of pets comprises mixing 10-20% of bacillus subtilis fermentation product, 2-5% of synergistic accelerator, 3-6% of stabilizer and 75-85% of carrier, dispersing uniformly, and granulating to obtain hair bulb remover for improving gastrointestinal health of pets.
In summary, the application has the following beneficial effects:
1. according to the hair bulb remover disclosed by the application, the bacillus subtilis fermentation product is innovatively added, and the bacillus subtilis fermentation product has higher keratinase activity and better acid resistance, so that the hair bulb remover can keep better stability in intestines and stomach of dogs and cats, can be specifically combined with keratin in hair, plays a role in degrading keratin in hair bulbs, can help to improve intestines and stomach health of dogs and promote digestion and absorption.
2. The bacillus subtilis fermentation product, the synergistic accelerator and the stabilizer are matched to promote the discharge of the hairballs in intestines and stomach of dogs in a digestion and excretion mode, reduce the condition of vomit discharge of the hairballs of dogs, and can effectively prevent the occurrence of pet hairball diseases.
3. The hair bulb remover consists of a bacillus subtilis fermentation product, a synergist, a stabilizer and a carrier, does not contain mineral oil or vegetable oil components, and is not easy to stimulate intestines and stomach of pets.
Detailed Description
Example 1
A preparation method of hair bulb remover for improving gastrointestinal health of pets comprises mixing 10kg of bacillus subtilis fermentation product, 3kg of synergistic accelerator, 3kg of stabilizer and 84kg of carrier uniformly, and making into hair bulb remover granule. Wherein the synergistic accelerator is vitamin C, the stabilizer is calcium lactate, the carrier is defatted rice bran, and the preparation method of the bacillus subtilis fermentation product comprises the following steps:
nutrient medium:
the nutrient agar medium mainly comprises 10g peptone, 3g beef extract, 5g sodium chloride and 1000ml distilled water. Mixing the above components uniformly, sterilizing at 121deg.C for 20min to obtain nutrient agar culture medium.
The fermentation medium mainly comprises 0.04g of sodium chloride, 0.02g of calcium gluconate, 0.1g of yucca extract, 2g of a mixture of cat hair and dog hair with a mass ratio of 1:1 and 1000mL of water, the components are uniformly mixed, potassium dihydrogen phosphate is added to adjust the pH value to 5.5, and the mixture is sterilized at 121 ℃ for 20min to obtain the fermentation medium.
Acid-resistant bacillus subtilis culture: inoculating 20ul of bacillus subtilis (purchased from Guangdong CycloKai microorganism technology Co., ltd.) into nutrient agar medium, shake culturing at 37deg.C for 24 hr, collecting 10-20% dilution of bacillus subtilis, adding 20% hydrochloric acid to adjust pH to 2.5, and standing culturing at 37deg.C for 2 hr to obtain acid-resistant bacillus subtilis.
Preparation of bacillus subtilis fermentation products: after the fermentation medium is sterilized, 1ml of bacillus subtilis is inoculated, the bacillus subtilis is cultured for 72 hours at 45 ℃ in a constant temperature incubator, after the fermentation is finished, the fermentation product is baked for 48 hours at 50 ℃, then crushed, screened by a 100-mesh screen, and the screened product is taken out to obtain the bacillus subtilis fermentation product, and the bacillus subtilis fermentation product is refrigerated below 10 ℃ for standby.
Example 2
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 1 in that: uniformly mixing 20kg of bacillus subtilis fermentation product, 5kg of synergistic accelerator, 6kg of stabilizer and 69kg of carrier to prepare the hair bulb remover particles.
The components of the fermentation medium are as follows: 0.06g of sodium chloride, 0.04g of calcium gluconate, 0.05g of yucca extract, 4g of a mixture of cat hair and dog hair in a mass ratio of 1:1 and 1000mL of water, and potassium dihydrogen phosphate is added to adjust the pH value to 6.5.
Example 3
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 1 in that: in the step of culturing acid-resistant bacillus subtilis, hydrochloric acid with the volume fraction of 20% is added to adjust the pH to 2.5, and then the bacillus subtilis is cultured for 4 hours.
Example 4
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 3 in that: in the preparation step of the bacillus subtilis fermentation product, the bacillus subtilis fermentation product is cultured for 96 hours at 45 ℃ in a constant temperature incubator.
Example 5
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 4 in that: the synergistic accelerator consists of 1.5kg of vitamin C and 1.5kg of taurine.
Example 6
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 4 in that: the synergistic accelerator is composed of 3kg taurine.
Example 7
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, differing from example 6 in that: the stabilizer was 1kg of calcium lactate and 2kg of calcium carbonate composition.
Example 8
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, differing from example 6 in that: the stabilizer was a combination of 1kg calcium lactate and 2kg calcium biphosphate.
Comparative example 1
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 1 in that: the fermentation medium consisted of: 0.04g of sodium chloride, 0.02g of calcium gluconate, 2g of a mixture of cat hair and dog hair in a mass ratio of 1:1 and 1000.1mL of water, and potassium dihydrogen phosphate is added to adjust the pH value to 5.5.
Comparative example 2
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 1 in that: the fermentation medium consisted of: 0.04g of sodium chloride, 0.1g of yucca extract, 2g of a mixture of cat hair and dog hair in a mass ratio of 1:1 and 1000.02mL of water, and potassium dihydrogen phosphate is added to adjust the pH to 5.5.
Comparative example 3
A method for preparing a hair bulb remover for improving gastrointestinal health in pets, which is different from example 3 in that: in the preparation step of the bacillus subtilis fermentation product, the bacillus subtilis fermentation product is cultured for 120 hours at 45 ℃ in a constant temperature incubator.
Experiment
Keratin enzyme activity assay
1g cat hair is taken as a substrate, 1g of the substrate and 0.1g of the hair bulb remover are added into 5ml of hydrochloric acid with the pH value of 2.5, the mixture is reacted for 2 hours at the temperature of 45 ℃, the mixture is cooled to the room temperature, the substrate is filtered, and the OD is measured 280nm Values.
The test results are shown in Table 1.
TABLE 1
| Example 1 | Example 2 | Example 3 | Example 4 | Example 5 | Example 6 |
| 0.242 | 0.237 | 0.326 | 0.357 | 0.362 | 0.437 |
| Example 7 | Example 8 | Comparative example 1 | Comparative example 2 | Comparative example 3 | |
| 0.439 | 0.441 | 0.054 | 0.037 | 0.127 |
Experiment 2
The cat with 20 mild hair bulb diseases is selected, two cats are selected, the use effect of the hair bulb remover prepared in each example and the comparative example is tested correspondingly, 10g of the hair bulb remover is fed in the morning and evening, the number of vomit hairs, the hair content change in the feces and the appetite change condition of the cat are recorded after observing for 1 month.
The results are shown in Table 2
TABLE 2
By combining the keratinase activity of example 1 with that of comparative example 1-2 in tables 1 and 2 and comparing the change of cats after feeding, the keratinase activity of the hair bulb remover prepared in example 1 is obviously higher than that of comparative example 1-2, and the effects of yucca extract and calcium gluconate addition on enhancing the activity of keratinase in a more acidic environment are obvious, so that after the hair bulb remover is fed to cats, keratinase can interact with agglomerated hair bulbs in the gastrointestinal tract of the cats, the keratins in the hair can be specifically degraded, the hair can be discharged out of the cats in a excretory mode, and the occurrence of cat vomiting hair bulbs can be reduced.
Comparison of the keratinase activity of example 3 with that of comparative example 3 and the changes in cats following feeding in combination with tables 1 and 2 shows that comparative example 3 has an extended fermentation time of the bacillus subtilis in the bacillus subtilis fermentation product preparation step compared with example 3, resulting in a reduced keratinase activity, and that after eating by cats, the symptoms of pilling are still more pronounced, indicating that the keratinase activity is highest for 72-96 hours.
The present embodiment is only for explanation of the present application and is not to be construed as limiting the present application, and modifications to the present embodiment, which may not creatively contribute to the present application as required by those skilled in the art after reading the present specification, are all protected by patent laws within the scope of claims of the present application.
Claims (5)
1. A hair bulb remover for improving gastrointestinal health in pets, characterized by: the composite material consists of the following components in percentage by mass: 10-20% of bacillus subtilis fermentation product, 3-5% of synergistic accelerator, 3-6% of stabilizer and 69-85% of carrier;
the preparation method of the bacillus subtilis fermentation product comprises the following steps:
culturing bacillus subtilis: inoculating bacillus subtilis into a nutrient agar culture medium, performing shake culture at 36.5-37.5 ℃ for 24-48h, taking 10-20% diluent of the bacillus subtilis, adjusting the pH to 2.5-3.5, and performing stationary culture at 36.5-37.5 ℃ for 36-48h to obtain the bacillus subtilis;
preparation of bacillus subtilis fermentation products:
adding mixture of cat hair and dog hair, calcium gluconate and Yucca extract into fermentation medium, sterilizing, inoculating Bacillus subtilis, fermenting at 40-50deg.C for 72-96 hr, oven drying, pulverizing, sieving to obtain Bacillus subtilis fermentation product, and refrigerating;
the fermentation medium is composed of: adding sodium chloride 0.04-0.06%, calcium gluconate 0.02-0.04%, yucca extract 0.01-0.03%, and mixture of cat hair and dog hair 1-2% into 1000ml water, and adjusting pH to 5.5-6.5;
the synergistic accelerator is one or two of vitamin C and taurine;
the stabilizer is one or more of calcium lactate, calcium biphosphate and calcium carbonate.
2. A hair bulb remover for improving the health of the gastrointestinal tract of pets according to claim 1, wherein: the synergistic accelerator is taurine with the mass percentage of at least 3 percent.
3. A hair bulb remover for improving the health of the gastrointestinal tract of pets according to claim 1, wherein: the stabilizer is a combination of calcium dihydrogen phosphate and calcium lactate according to the mass ratio of 2-3:1.
4. A hair bulb remover for improving the health of the gastrointestinal tract of pets according to claim 1, wherein: the carrier is defatted rice bran.
5. A method of preparing a hair bulb remover for improving gastrointestinal health in pets according to any of claims 1-4, wherein: mixing 10-20% of bacillus subtilis fermentation product, 3-5% of synergistic accelerator, 3-6% of stabilizer and 69-85% of carrier, uniformly dispersing, and granulating to obtain the hair bulb remover for improving gastrointestinal health of pets.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6080403A (en) * | 1998-06-18 | 2000-06-27 | Star-Kist Foods, Inc. | Protease containing hairball remedy and use thereof |
| JP2003226613A (en) * | 2002-01-31 | 2003-08-12 | Ichimaru Pharcos Co Ltd | Protease activity-promoting agent |
| CN102517235A (en) * | 2011-12-27 | 2012-06-27 | 湖南农业大学 | Bacillus subtilis |
| CN107739726A (en) * | 2017-11-15 | 2018-02-27 | 浙江科技学院 | A kind of bacillus subtilis and its application |
| CN109306329A (en) * | 2018-03-16 | 2019-02-05 | 广州大峰收技术服务有限公司 | Bacillus subtilis and its application |
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Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US6080403A (en) * | 1998-06-18 | 2000-06-27 | Star-Kist Foods, Inc. | Protease containing hairball remedy and use thereof |
| JP2003226613A (en) * | 2002-01-31 | 2003-08-12 | Ichimaru Pharcos Co Ltd | Protease activity-promoting agent |
| CN102517235A (en) * | 2011-12-27 | 2012-06-27 | 湖南农业大学 | Bacillus subtilis |
| CN107739726A (en) * | 2017-11-15 | 2018-02-27 | 浙江科技学院 | A kind of bacillus subtilis and its application |
| CN109306329A (en) * | 2018-03-16 | 2019-02-05 | 广州大峰收技术服务有限公司 | Bacillus subtilis and its application |
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