CN115606593B - Calotropis gigantea cutting nutrient and cutting method - Google Patents
Calotropis gigantea cutting nutrient and cutting method Download PDFInfo
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- CN115606593B CN115606593B CN202211277576.3A CN202211277576A CN115606593B CN 115606593 B CN115606593 B CN 115606593B CN 202211277576 A CN202211277576 A CN 202211277576A CN 115606593 B CN115606593 B CN 115606593B
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- cutting
- cantaloupe
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- calotropis gigantea
- rooting
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- 238000005520 cutting process Methods 0.000 title claims abstract description 108
- 241000208250 Calotropis gigantea Species 0.000 title claims abstract description 47
- 235000015097 nutrients Nutrition 0.000 title claims abstract description 41
- 238000000034 method Methods 0.000 title claims abstract description 23
- 235000009847 Cucumis melo var cantalupensis Nutrition 0.000 claims abstract description 41
- YGSDEFSMJLZEOE-UHFFFAOYSA-N salicylic acid Chemical compound OC(=O)C1=CC=CC=C1O YGSDEFSMJLZEOE-UHFFFAOYSA-N 0.000 claims abstract description 34
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 19
- 230000000813 microbial effect Effects 0.000 claims abstract description 19
- 239000011159 matrix material Substances 0.000 claims abstract description 18
- 235000007164 Oryza sativa Nutrition 0.000 claims abstract description 17
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 claims abstract description 17
- FJKROLUGYXJWQN-UHFFFAOYSA-N papa-hydroxy-benzoic acid Natural products OC(=O)C1=CC=C(O)C=C1 FJKROLUGYXJWQN-UHFFFAOYSA-N 0.000 claims abstract description 17
- 235000009566 rice Nutrition 0.000 claims abstract description 17
- 229960004889 salicylic acid Drugs 0.000 claims abstract description 17
- 239000000052 vinegar Substances 0.000 claims abstract description 17
- 235000021419 vinegar Nutrition 0.000 claims abstract description 17
- 244000241257 Cucumis melo Species 0.000 claims abstract 11
- 240000007594 Oryza sativa Species 0.000 claims abstract 3
- 244000063299 Bacillus subtilis Species 0.000 claims description 12
- 235000014469 Bacillus subtilis Nutrition 0.000 claims description 12
- 241000222120 Candida <Saccharomycetales> Species 0.000 claims description 12
- 241000589516 Pseudomonas Species 0.000 claims description 12
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 12
- 239000002689 soil Substances 0.000 claims description 10
- 244000060011 Cocos nucifera Species 0.000 claims description 9
- 235000013162 Cocos nucifera Nutrition 0.000 claims description 9
- 239000003415 peat Substances 0.000 claims description 9
- 239000010451 perlite Substances 0.000 claims description 9
- 235000019362 perlite Nutrition 0.000 claims description 9
- 238000002791 soaking Methods 0.000 claims description 9
- YZCKVEUIGOORGS-NJFSPNSNSA-N Tritium Chemical group [3H] YZCKVEUIGOORGS-NJFSPNSNSA-N 0.000 claims description 3
- 238000009395 breeding Methods 0.000 claims description 3
- 230000001488 breeding effect Effects 0.000 claims description 3
- 238000010790 dilution Methods 0.000 claims description 3
- 239000012895 dilution Substances 0.000 claims description 3
- 241000894006 Bacteria Species 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 13
- 230000004083 survival effect Effects 0.000 abstract description 13
- QTBSBXVTEAMEQO-UHFFFAOYSA-N Acetic acid Chemical compound CC(O)=O QTBSBXVTEAMEQO-UHFFFAOYSA-N 0.000 abstract description 9
- 230000000694 effects Effects 0.000 abstract description 9
- 230000001737 promoting effect Effects 0.000 abstract description 5
- 208000035240 Disease Resistance Diseases 0.000 abstract description 4
- 230000009545 invasion Effects 0.000 abstract description 4
- 244000005700 microbiome Species 0.000 abstract description 4
- 230000002411 adverse Effects 0.000 abstract description 3
- 230000007613 environmental effect Effects 0.000 abstract description 3
- 235000016709 nutrition Nutrition 0.000 abstract description 3
- 230000035764 nutrition Effects 0.000 abstract description 3
- 238000004659 sterilization and disinfection Methods 0.000 abstract 1
- 244000064895 Cucumis melo subsp melo Species 0.000 description 33
- 241000209094 Oryza Species 0.000 description 14
- 230000000052 comparative effect Effects 0.000 description 10
- 206010052428 Wound Diseases 0.000 description 6
- 208000027418 Wounds and injury Diseases 0.000 description 6
- 230000012010 growth Effects 0.000 description 4
- 239000000463 material Substances 0.000 description 3
- 239000008267 milk Substances 0.000 description 3
- 210000004080 milk Anatomy 0.000 description 3
- 235000013336 milk Nutrition 0.000 description 3
- 230000009286 beneficial effect Effects 0.000 description 2
- 238000007796 conventional method Methods 0.000 description 2
- 239000000835 fiber Substances 0.000 description 2
- 239000002207 metabolite Substances 0.000 description 2
- 238000009629 microbiological culture Methods 0.000 description 2
- 238000002156 mixing Methods 0.000 description 2
- 238000004321 preservation Methods 0.000 description 2
- 230000002035 prolonged effect Effects 0.000 description 2
- 239000002994 raw material Substances 0.000 description 2
- 239000000243 solution Substances 0.000 description 2
- 239000000758 substrate Substances 0.000 description 2
- 238000005303 weighing Methods 0.000 description 2
- 229920000742 Cotton Polymers 0.000 description 1
- HEBKCHPVOIAQTA-QWWZWVQMSA-N D-arabinitol Chemical compound OC[C@@H](O)C(O)[C@H](O)CO HEBKCHPVOIAQTA-QWWZWVQMSA-N 0.000 description 1
- SHZGCJCMOBCMKK-UHFFFAOYSA-N D-mannomethylose Natural products CC1OC(O)C(O)C(O)C1O SHZGCJCMOBCMKK-UHFFFAOYSA-N 0.000 description 1
- 244000299452 Gouania lupuloides Species 0.000 description 1
- 235000000292 Gouania lupuloides Nutrition 0.000 description 1
- SHZGCJCMOBCMKK-JFNONXLTSA-N L-rhamnopyranose Chemical compound C[C@@H]1OC(O)[C@H](O)[C@H](O)[C@H]1O SHZGCJCMOBCMKK-JFNONXLTSA-N 0.000 description 1
- PNNNRSAQSRJVSB-UHFFFAOYSA-N L-rhamnose Natural products CC(O)C(O)C(O)C(O)C=O PNNNRSAQSRJVSB-UHFFFAOYSA-N 0.000 description 1
- 241000130764 Tinea Species 0.000 description 1
- 208000002474 Tinea Diseases 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000006978 adaptation Effects 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 206010006451 bronchitis Diseases 0.000 description 1
- 229940097217 cardiac glycoside Drugs 0.000 description 1
- 239000002368 cardiac glycoside Substances 0.000 description 1
- 230000000249 desinfective effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000003113 dilution method Methods 0.000 description 1
- 238000001035 drying Methods 0.000 description 1
- 208000001848 dysentery Diseases 0.000 description 1
- 235000013399 edible fruits Nutrition 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 210000003608 fece Anatomy 0.000 description 1
- 239000000945 filler Substances 0.000 description 1
- 238000010413 gardening Methods 0.000 description 1
- 210000004209 hair Anatomy 0.000 description 1
- 239000007788 liquid Substances 0.000 description 1
- 239000010871 livestock manure Substances 0.000 description 1
- HEBKCHPVOIAQTA-UHFFFAOYSA-N meso ribitol Natural products OCC(O)C(O)C(O)CO HEBKCHPVOIAQTA-UHFFFAOYSA-N 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- AJDUTMFFZHIJEM-UHFFFAOYSA-N n-(9,10-dioxoanthracen-1-yl)-4-[4-[[4-[4-[(9,10-dioxoanthracen-1-yl)carbamoyl]phenyl]phenyl]diazenyl]phenyl]benzamide Chemical compound O=C1C2=CC=CC=C2C(=O)C2=C1C=CC=C2NC(=O)C(C=C1)=CC=C1C(C=C1)=CC=C1N=NC(C=C1)=CC=C1C(C=C1)=CC=C1C(=O)NC1=CC=CC2=C1C(=O)C1=CC=CC=C1C2=O AJDUTMFFZHIJEM-UHFFFAOYSA-N 0.000 description 1
- 235000012149 noodles Nutrition 0.000 description 1
- 230000035699 permeability Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 244000000003 plant pathogen Species 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000029058 respiratory gaseous exchange Effects 0.000 description 1
- 239000004576 sand Substances 0.000 description 1
- 230000001568 sexual effect Effects 0.000 description 1
- 208000017520 skin disease Diseases 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 239000002904 solvent Substances 0.000 description 1
- 230000001954 sterilising effect Effects 0.000 description 1
- 229930002534 steroid glycoside Natural products 0.000 description 1
- 150000008143 steroidal glycosides Chemical class 0.000 description 1
- 239000004094 surface-active agent Substances 0.000 description 1
- 230000002195 synergetic effect Effects 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 238000011282 treatment Methods 0.000 description 1
- 210000002268 wool Anatomy 0.000 description 1
- 239000001043 yellow dye Substances 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/36—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
- A01N37/38—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
- A01N37/40—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C21/00—Methods of fertilising, sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G17/00—Cultivation of hops, vines, fruit trees, or like trees
- A01G17/005—Cultivation methods
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G2/00—Vegetative propagation
- A01G2/10—Vegetative propagation by means of cuttings
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/10—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
- A01G24/12—Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
- A01G24/15—Calcined rock, e.g. perlite, vermiculite or clay aggregates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G24/00—Growth substrates; Culture media; Apparatus or methods therefor
- A01G24/20—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
- A01G24/22—Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
- A01G24/25—Dry fruit hulls or husks, e.g. chaff or coir
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N37/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
- A01N37/02—Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N59/00—Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
- A01N59/26—Phosphorus; Compounds thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/22—Bacillus
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/20—Bacteria; Substances produced thereby or obtained therefrom
- A01N63/27—Pseudomonas
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/32—Yeast
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P3/00—Fungicides
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- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Pest Control & Pesticides (AREA)
- Dentistry (AREA)
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- Agronomy & Crop Science (AREA)
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- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
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Abstract
The invention provides a cutting nutrient and a cutting method for Calotropis gigantea, and relates to the technical field of plant cultivation. The cutting nutrient for the Calotropis gigantea comprises the following components: salicylic acid, rice vinegar, dipotassium hydrogen phosphate and microbial agents, wherein the salicylic acid not only promotes rooting and can strengthen roots, but also can prevent rotten roots; the acetic acid contained in the rice vinegar has the effects of sterilization and disinfection, and simultaneously can provide nutrition components for the cutting wound of the cantaloupe and promote rooting; dipotassium hydrogen phosphate can improve plant stress resistance and adapt to the capability of external adverse environmental conditions; the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in a matrix, and achieves the effects of promoting rooting and improving survival rate. Experimental results show that after the cutting wound of the cantaloupe is soaked in the cantaloupe cutting nutrient before cutting, the root length, rooting rate, nursery yield and survival rate of the cantaloupe can be remarkably improved.
Description
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a cutting nutrient for calotropis gigantea and a cutting method.
Background
Calotropis gigantea (school name: calotropis gigantea (L.) Dry.ex Ait.f.) is an upright shrub, up to 3 meters, whole plant with milk; the stems are yellow and white, the branches are thick and strong, and the young branch parts are grey and white fluff. The leaf is oval or elliptical, and has a length of 8-20 cm and a width of 3.5-9.5 cm. The shape of the umbrella inflorescence, axillary growth and top growth; inflorescences and peduncles are colored grey and white with villi. Setting a whole length; the seeds are wide oval, 5mm long and 3 mm wide; the seed hair length is 2.5 cm. The flower and fruit period is almost year round. The stem skin fiber can be used for paper making, rope making and artificial cotton, and bast fiber fabrics and sacks are woven; the wool can be used as velvet raw material and filler. The milk of the stem and leaf is toxic, contains a plurality of cardiac glycosides, and can be used for treating skin diseases, dysentery, rheumatism and bronchitis; pi can treat tinea. The milk can be used as gum material after drying, and can also be used for preparing tanning material and yellow dye. The whole plant can be used as green manure.
The industrial, agricultural and medical uses of the Calotropis gigantea have been widely studied, however, the current planting of the Calotropis gigantea is limited by propagation means. Until now, seed propagation methods, namely sexual propagation methods, are adopted for propagation of the Calotropis gigantea, and the current asexual propagation methods of the Calotropis gigantea are rarely reported.
The plant asexual propagation method comprises cutting, layering, grafting, separating plants and the like. The cutting is also called cutting, is a common propagation method for cultivating plants, and the stems, leaves, roots, buds and the like of the plants are cut (called cutting in gardening), or are inserted into soil, sand or are soaked in water, and the plants can be planted after rooting, so that the plants become independent new plants. The temperature suitable for growth of the calotropis gigantea is 20-35 ℃, and the created noodles are easy to be infected in the cutting process at the temperature, so that the phenomena of no rooting or even rotting root, prolonged rooting time and low survival rate occur. Therefore, a method for improving the rooting rate and the survival rate of cutting at a proper growth temperature of the cantaloupe is needed.
Disclosure of Invention
The invention aims to provide a cutting nutrient and a cutting method for Calotropis gigantea, which can obviously improve root length, rooting rate, nursery-out rate and survival rate of Calotropis gigantea.
In order to achieve the above object, the present invention provides the following technical solutions:
The invention provides a cutting nutrient for calotropis gigantea, which comprises the following components in parts by weight: 5-15 parts of salicylic acid, 10-20 parts of rice vinegar, 8-12 parts of dipotassium hydrogen phosphate and 3-9 parts of microbial agent.
Preferably, the microbial agent comprises: the weight ratio of the pseudomonas N21 to the candida FYAT to the bacillus subtilis B9601 to the Y2 is (1-3) to (1-3).
More preferably, the microbial agent has a bacterial count of more than 10 9/g.
The invention also provides a cutting method of the cantaloupe, which comprises the following steps: selecting 1-3 year-old cantaloupe, cutting main stems into stem segments with inclined openings at the lower ends of 5-13cm, soaking the lower ends of the stem segments in the cantaloupe cutting nutrient, and inserting the stem segments into a cutting matrix for cultivation.
Preferably, the stem segment comprises 1-3 axillary buds and 1-3 leaves.
Preferably, the dilution factor of the cutting nutrient for the Calotropis gigantea is 200-500.
Preferably, the soaking time is 10-20min.
Preferably, the cutting medium comprises: coconut chaff, perlite and peat soil; the mass ratio of the coconut coir, the perlite and the peat soil is (8-15): 3-8): 2-5; .
Preferably, the water content of the cutting matrix is 50% -60%; the cultivation temperature of the cuttage is 20-35 ℃.
The invention also provides application of the cutting nutrient for the cantaloupe or the cutting method for the cantaloupe in breeding of the cantaloupe.
Compared with the prior art, the invention has the following beneficial effects:
The invention provides a cutting nutrient for calotropis gigantea, which comprises the following components: salicylic acid, rice vinegar, dipotassium hydrogen phosphate and microbial agents, wherein the salicylic acid not only promotes rooting and can strengthen roots, but also can prevent rotten roots; the acetic acid contained in the rice vinegar has the effects of sterilizing and disinfecting, and simultaneously can provide nutrition components for the cutting wound of the cantaloupe and promote rooting; dipotassium hydrogen phosphate can improve plant stress resistance and adapt to the capability of external adverse environmental conditions; the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in a matrix, and achieves the effects of promoting rooting and improving survival rate. Experimental results show that after the cutting wound of the cantaloupe is soaked in the cantaloupe cutting nutrient before cutting, the root length, rooting rate, nursery yield and survival rate of the cantaloupe can be remarkably improved.
Detailed Description
The invention provides a cutting nutrient for calotropis gigantea, which comprises the following components in parts by weight: 5-15 parts of salicylic acid, 10-20 parts of rice vinegar, 8-12 parts of dipotassium hydrogen phosphate and 3-9 parts of microbial agent.
The cutting nutrient for the cantaloupe disclosed by the invention more preferably comprises the following components in parts by weight: 10 parts of salicylic acid, 15 parts of rice vinegar, 10 parts of dipotassium hydrogen phosphate and 6 parts of microbial agent.
In the present invention, the microbial agent preferably comprises: the weight ratio of the pseudomonas N21 to the candida FYAT to the bacillus subtilis B9601-Y2 is preferably (1-3): 1-3, more preferably 2:2:2; the number of bacteria in the microbial agent is preferably more than 10 9/g. The Pseudomonas N21 has a preservation number of CCTCCNO: M2016180, and is purchased from China center for type culture Collection; the candida FYAT1104 has a preservation number of CGMCC No.4761 and is purchased from the China general microbiological culture Collection center; the bacillus subtilis B9601-Y2 has a collection number of CGMCC No.0954 and is purchased from the China general microbiological culture Collection center (China Committee for culture Collection). The metabolite rhamnose and the metabolite arabitol of candida FYAT1104 in the cutting process of the pseudomonas N21 can be used as a surfactant, so that components in the nutrient can be fixed on cutting wounds of the cantaloupe, and the action time is prolonged to achieve the effects of promoting cutting rooting and improving the survival rate; the bacillus subtilis B9601-Y2 can effectively promote plant growth and rooting, has a good control effect on a plurality of soil-borne plant pathogens, and has good stress resistance. According to the invention, the substrate is not required to be sterilized in the cutting process, and the microbial agent has a synergistic effect among the pseudomonas N21, the candida FYAT and the bacillus subtilis B9601-Y2, and the addition of the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in the substrate, and achieves the effects of promoting rooting and improving survival rate.
In the invention, the salicylic acid not only promotes rooting and can strengthen the root, but also can prevent root rot; the acetic acid contained in the rice vinegar can sterilize and disinfect, and simultaneously can provide nutrition for the cutting wound of the cantaloupe so as to promote rooting; the dipotassium hydrogen phosphate can improve plant stress resistance and adapt to the capability of external adverse environmental conditions; the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in a matrix, and achieves the effects of promoting rooting and improving survival rate.
The preparation method of the cutting nutrient for the Calotropis gigantea is not particularly limited and can be achieved by adopting a conventional method in the field. The cutting nutrient for the Calotropis gigantea comprises a solid component and a liquid component, so that the cutting nutrient is prepared before cutting. As an embodiment, salicylic acid, rice vinegar, dipotassium hydrogen phosphate and microbial agent are mixed.
The invention also provides a cutting method of the cantaloupe, which comprises the following steps: selecting 1-3 year-old cantaloupe, cutting main stems into stem segments with inclined openings at the lower ends of 5-13cm, soaking the lower ends of the stem segments in the cantaloupe cutting nutrient, and inserting the stem segments into a cutting matrix for cultivation.
In the invention, the calotropis gigantea preferably selects 2-year-old main stems, and the stem segments preferably comprise 1-3 axillary buds, 1-3 leaves, more preferably 2 axillary buds and 2 leaves; the dilution factor of the cutting nutrient for Calotropis gigantea is preferably 200-500, more preferably 300, and the solvent in the dilution process is water; the soaking time is preferably 10-20min, more preferably 15min.
In the present invention, the cutting medium preferably comprises: coconut chaff, perlite and peat soil; the mass ratio of the coconut coir, the perlite and the peat soil is preferably (8-15): (3-8): (2-5), more preferably 10:5:4; the moisture content of the cutting matrix is preferably 50% -60%, more preferably 55%; the cultivation temperature of the cuttage is preferably 20-35 ℃, more preferably 30 ℃. Too much and too little water content in the cutting medium is detrimental to rooting of the wound. The cuttage matrix has good porosity, air permeability and water retention, can ensure that the calotropis gigantea is not easy to rot at a higher temperature, is more beneficial to root system respiration after rooting, and is not easy to cause choking and death.
The invention also provides application of the cutting nutrient for the cantaloupe or the cutting method for the cantaloupe in breeding of the cantaloupe.
In the present invention, all raw material components are commercially available products well known to those skilled in the art unless specified otherwise.
The technical solutions of the present invention will be clearly and completely described in the following in connection with the embodiments of the present invention. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Calotropis gigantea cutting nutrient
Accurately weighing 10g of salicylic acid, 15g of rice vinegar, 10g of dipotassium hydrogen phosphate, 21 g of pseudomonas N, FYAT g of candida FYAT g 1104 and 22 g of bacillus subtilis B9601-Y, and mixing.
Example 2
Calotropis gigantea cutting nutrient
Accurately weighing 5g of salicylic acid, 10g of rice vinegar, 8g of dipotassium hydrogen phosphate, 21 g of pseudomonas N, FYAT g of candida FYAT g of 1104 g and 9601-Y2 1g of bacillus subtilis, and mixing.
Example 3
Calotropis gigantea cutting nutrient
15G of salicylic acid, 20g of rice vinegar, 12g of dipotassium hydrogen phosphate, 21 g of pseudomonas N, FYAT g of candida FYAT g 1104 and 9601-Y2 3g of bacillus subtilis are accurately weighed and mixed.
Example 4
Cutting of Calotropis gigantea
(1) Selecting a 2-year-old Calotropis gigantea plant, and cutting a main stem into a stem section with a bevel at the lower end of 10cm, wherein the stem section contains 2 axillary buds and 2 leaves;
(2) Inserting the stem into the cutting nutrient of the Calotropis gigantea of the embodiment 1 diluted 300 times by water, and soaking for 15min;
(3) Putting coconut coir, perlite and peat soil serving as a cutting matrix into a seedling pot with the specification diameter of 10 multiplied by the height of 10 multiplied by the bottom diameter of 11 multiplied by 8.5cm according to the weight ratio of 10:5:4, and keeping the water content of the cutting matrix to be 55%;
(4) The soaked stem segments are inserted into a cutting matrix and then cultivated in an environment of 30 ℃.
Example 5
Cutting of Calotropis gigantea
(1) Selecting 1-year-old Calotropis gigantea plant, and cutting the main stem into stem segments with a bevel opening at the lower end of 5cm, wherein the stem segments comprise 1 axillary bud and 3 leaves;
(2) Inserting the stem into the cutting nutrient of the Calotropis gigantea of the embodiment 2 diluted 200 times by water, and soaking for 10min;
(3) Putting coconut coir, perlite and peat soil serving as a cutting matrix into a seedling pot with the specification diameter of 10 multiplied by the height of 10 multiplied by the bottom diameter of 11 multiplied by 8.5cm according to the weight ratio of 8:3:2, and keeping the water content of the cutting matrix to be 60%;
(4) The soaked stem segments are inserted into a cutting matrix and then cultivated in an environment of 20 ℃.
Example 6
Cutting of Calotropis gigantea
(1) Selecting a 3-year-old cantaloupe plant, and cutting a main stem into a stem section with a 13cm lower end bevel, wherein the stem section contains 3 axillary buds and 1 leaf;
(2) Inserting the stem into the cutting nutrient of the Calotropis gigantea of the embodiment 3 diluted 500 times by water, and soaking for 20min;
(3) Putting coconut coir, perlite and peat soil serving as a cutting matrix into a seedling pot with the specification diameter of 10 multiplied by the height of 10 multiplied by the bottom diameter of 11 multiplied by 8.5cm according to the weight ratio of 15:8:5, and keeping the water content of the cutting matrix to be 50%;
(4) The soaked stem segments are inserted into a cutting matrix and then cultivated in an environment of 30 ℃.
Comparative example 1
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise microbial agents, the mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of salicylic acid to rice vinegar to dipotassium hydrogen phosphate is 2:3:2.
Comparative example 2
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise salicylic acid, the total mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of rice vinegar, dipotassium hydrogen phosphate, pseudomonas N21, candida FYAT1104 and bacillus subtilis B9601-Y2 is 15:10:2:2:2.
Comparative example 3
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise rice vinegar, the total mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of salicylic acid, dipotassium hydrogen phosphate, pseudomonas N21, candida FYAT1104 and bacillus subtilis B9601-Y2 is 5:5:1:1:1.
Comparative example 4
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise dipotassium hydrogen phosphate, the total mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of salicylic acid, rice vinegar, pseudomonas N21, candida FYAT1104 and bacillus subtilis B9601-Y2 is 10:15:2:2:2.
Comparative example 5
The specific embodiment is the same as example 4, except that the cutting nutrient of Calotropis gigantea is replaced with water.
The raw cantaloupe plants referred to in examples 4-6 and comparative examples 1-5 were obtained from a cantaloupe seed planting base in Honghe county of Yunnan, and transplanted to a plateau forestry institute laboratory of China forestry science institute as a cantaloupe material to be subjected to cutting propagation by seed propagation seedlings. The raw cantaloupe plants of the examples 4-6 and the comparative examples 1-5 are subjected to cuttage on the day 08 and 15 of 2020, the diameters and wound areas of cuttage stem segments are not obviously different, the cuttage number of each example or comparative example is 100, and the management after cuttage is carried out according to a conventional method in the field.
Experimental example 1
The cuttings of examples 4 to 6 and comparative examples 1 to 5 were examined after 10 days of cutting, and the average root number, average root length, nursery rate and survival rate after 3 months of transplanting were measured.
Root number means the number of white roots (roots) protruding from the base of the slip; average root number = root number sum of root stem segments/root stem segment number 100%; root length means the length (cm) of the longest root system measured from the root of the slip; average root length = root length sum of root stem segments/root stem segment number 100%; rooting rate = rooting stem/100 x 100%; nursery rate = suitable transplanted stem segments/100 x 100%; survival = surviving stem segments after transplanting/stem segments suitable for transplanting 100%. The specific results are shown in Table 1.
TABLE 1 Effect of different treatments on Calotropis gigantea cutting parameters
As shown in Table 1, the cutting method of Calotropis gigantea of the present invention was significantly improved in average root number, average root length, rooting rate, nursery rate and survival rate compared with comparative examples 1 to 5 after cutting for 10 days.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.
Claims (8)
1. The cutting nutrient for the cantaloupe is characterized by comprising the following components in parts by weight: 5-15 parts of salicylic acid, 10-20 parts of rice vinegar, 8-12 parts of dipotassium hydrogen phosphate and 3-9 parts of microbial agent;
The microbial agent comprises the following components: the weight ratio of the pseudomonas N21 to the candida FYAT to the bacillus subtilis B9601 to the Y2 is (1-3) to (1-3);
The number of bacteria in the microbial agent is more than 10 9 /g.
2. The cutting method of the cantaloupe is characterized by comprising the following steps of:
Cutting 1-3 year-old Calotropis gigantea main stem into stem segments with inclined openings at the lower ends of 5-13 cm, soaking the lower ends of the stem segments in the Calotropis gigantea cutting nutrient according to claim 1, and inserting the stem segments into a cutting matrix for cultivation.
3. The cutting method of Calotropis gigantea according to claim 2, wherein the stem section comprises 1-3 axillary buds and 1-3 leaves.
4. The cutting method of the cantaloupe according to claim 2, wherein the dilution factor of the cantaloupe cutting nutrient is 200-500.
5. The cutting method of Calotropis gigantea according to claim 2, wherein the soaking time is 10-20 min.
6. The cutting method of calotropis gigantea according to claim 2, wherein the cutting medium comprises: coconut chaff, perlite and peat soil; the mass ratio of the coconut coir, the perlite and the peat soil is (8-15)/(3-8)/(2-5).
7. The cutting method of Calotropis gigantea according to claim 2, wherein the water content of the cutting medium is 50% -60%; the cultivation temperature of the cuttage is 20-35 ℃.
8. Use of the cantaloupe cutting nutrient of claim 1 or the cantaloupe cutting method of any one of claims 2-7 in cantaloupe breeding.
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CN1554744A (en) * | 2003-12-28 | 2004-12-15 | 何月秋 | Bacillus subtilis strain |
CN102533571A (en) * | 2011-12-30 | 2012-07-04 | 安徽丰原发酵技术工程研究有限公司 | Tropic candida sp. mutant strain and application thereof |
CN106119148A (en) * | 2016-06-07 | 2016-11-16 | 江苏联海生物科技有限公司 | One pseudomonas and application thereof |
CN107750687A (en) * | 2017-11-07 | 2018-03-06 | 广西壮族自治区林业科学研究院 | A kind of container seedling culture method bred using Calotropis gigantea branch cutting |
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Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
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CN1554744A (en) * | 2003-12-28 | 2004-12-15 | 何月秋 | Bacillus subtilis strain |
CN102533571A (en) * | 2011-12-30 | 2012-07-04 | 安徽丰原发酵技术工程研究有限公司 | Tropic candida sp. mutant strain and application thereof |
CN106119148A (en) * | 2016-06-07 | 2016-11-16 | 江苏联海生物科技有限公司 | One pseudomonas and application thereof |
CN107750687A (en) * | 2017-11-07 | 2018-03-06 | 广西壮族自治区林业科学研究院 | A kind of container seedling culture method bred using Calotropis gigantea branch cutting |
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