CN115606593B - Calotropis gigantea cutting nutrient and cutting method - Google Patents

Calotropis gigantea cutting nutrient and cutting method Download PDF

Info

Publication number
CN115606593B
CN115606593B CN202211277576.3A CN202211277576A CN115606593B CN 115606593 B CN115606593 B CN 115606593B CN 202211277576 A CN202211277576 A CN 202211277576A CN 115606593 B CN115606593 B CN 115606593B
Authority
CN
China
Prior art keywords
cutting
cantaloupe
nutrient
calotropis gigantea
rooting
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
CN202211277576.3A
Other languages
Chinese (zh)
Other versions
CN115606593A (en
Inventor
马宏
万友名
李金仙
李正红
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences
Original Assignee
Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences filed Critical Institute Of Plateau Forestry Chinese Academy Of Forestry Sciences
Priority to CN202211277576.3A priority Critical patent/CN115606593B/en
Publication of CN115606593A publication Critical patent/CN115606593A/en
Application granted granted Critical
Publication of CN115606593B publication Critical patent/CN115606593B/en
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/36Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids
    • A01N37/38Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system
    • A01N37/40Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids containing at least one carboxylic group or a thio analogue, or a derivative thereof, and a singly bound oxygen or sulfur atom attached to the same carbon skeleton, this oxygen or sulfur atom not being a member of a carboxylic group or of a thio analogue, or of a derivative thereof, e.g. hydroxy-carboxylic acids having at least one oxygen or sulfur atom attached to an aromatic ring system having at least one carboxylic group or a thio analogue, or a derivative thereof, and one oxygen or sulfur atom attached to the same aromatic ring system
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01CPLANTING; SOWING; FERTILISING
    • A01C21/00Methods of fertilising, sowing or planting
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G17/00Cultivation of hops, vines, fruit trees, or like trees
    • A01G17/005Cultivation methods
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G2/00Vegetative propagation
    • A01G2/10Vegetative propagation by means of cuttings
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/10Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material
    • A01G24/12Growth substrates; Culture media; Apparatus or methods therefor based on or containing inorganic material containing soil minerals
    • A01G24/15Calcined rock, e.g. perlite, vermiculite or clay aggregates
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01GHORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
    • A01G24/00Growth substrates; Culture media; Apparatus or methods therefor
    • A01G24/20Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material
    • A01G24/22Growth substrates; Culture media; Apparatus or methods therefor based on or containing natural organic material containing plant material
    • A01G24/25Dry fruit hulls or husks, e.g. chaff or coir
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N37/00Biocides, pest repellants or attractants, or plant growth regulators containing organic compounds containing a carbon atom having three bonds to hetero atoms with at the most two bonds to halogen, e.g. carboxylic acids
    • A01N37/02Saturated carboxylic acids or thio analogues thereof; Derivatives thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N59/00Biocides, pest repellants or attractants, or plant growth regulators containing elements or inorganic compounds
    • A01N59/26Phosphorus; Compounds thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/22Bacillus
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/20Bacteria; Substances produced thereby or obtained therefrom
    • A01N63/27Pseudomonas
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N63/00Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
    • A01N63/30Microbial fungi; Substances produced thereby or obtained therefrom
    • A01N63/32Yeast
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P1/00Disinfectants; Antimicrobial compounds or mixtures thereof
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P21/00Plant growth regulators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01PBIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
    • A01P3/00Fungicides

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Environmental Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Plant Pathology (AREA)
  • Health & Medical Sciences (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Pest Control & Pesticides (AREA)
  • Dentistry (AREA)
  • Microbiology (AREA)
  • Chemical & Material Sciences (AREA)
  • Agronomy & Crop Science (AREA)
  • Inorganic Chemistry (AREA)
  • Biotechnology (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Botany (AREA)
  • Soil Sciences (AREA)
  • Developmental Biology & Embryology (AREA)
  • Cultivation Of Plants (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

The invention provides a cutting nutrient and a cutting method for Calotropis gigantea, and relates to the technical field of plant cultivation. The cutting nutrient for the Calotropis gigantea comprises the following components: salicylic acid, rice vinegar, dipotassium hydrogen phosphate and microbial agents, wherein the salicylic acid not only promotes rooting and can strengthen roots, but also can prevent rotten roots; the acetic acid contained in the rice vinegar has the effects of sterilization and disinfection, and simultaneously can provide nutrition components for the cutting wound of the cantaloupe and promote rooting; dipotassium hydrogen phosphate can improve plant stress resistance and adapt to the capability of external adverse environmental conditions; the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in a matrix, and achieves the effects of promoting rooting and improving survival rate. Experimental results show that after the cutting wound of the cantaloupe is soaked in the cantaloupe cutting nutrient before cutting, the root length, rooting rate, nursery yield and survival rate of the cantaloupe can be remarkably improved.

Description

Calotropis gigantea cutting nutrient and cutting method
Technical Field
The invention relates to the technical field of plant cultivation, in particular to a cutting nutrient for calotropis gigantea and a cutting method.
Background
Calotropis gigantea (school name: calotropis gigantea (L.) Dry.ex Ait.f.) is an upright shrub, up to 3 meters, whole plant with milk; the stems are yellow and white, the branches are thick and strong, and the young branch parts are grey and white fluff. The leaf is oval or elliptical, and has a length of 8-20 cm and a width of 3.5-9.5 cm. The shape of the umbrella inflorescence, axillary growth and top growth; inflorescences and peduncles are colored grey and white with villi. Setting a whole length; the seeds are wide oval, 5mm long and 3 mm wide; the seed hair length is 2.5 cm. The flower and fruit period is almost year round. The stem skin fiber can be used for paper making, rope making and artificial cotton, and bast fiber fabrics and sacks are woven; the wool can be used as velvet raw material and filler. The milk of the stem and leaf is toxic, contains a plurality of cardiac glycosides, and can be used for treating skin diseases, dysentery, rheumatism and bronchitis; pi can treat tinea. The milk can be used as gum material after drying, and can also be used for preparing tanning material and yellow dye. The whole plant can be used as green manure.
The industrial, agricultural and medical uses of the Calotropis gigantea have been widely studied, however, the current planting of the Calotropis gigantea is limited by propagation means. Until now, seed propagation methods, namely sexual propagation methods, are adopted for propagation of the Calotropis gigantea, and the current asexual propagation methods of the Calotropis gigantea are rarely reported.
The plant asexual propagation method comprises cutting, layering, grafting, separating plants and the like. The cutting is also called cutting, is a common propagation method for cultivating plants, and the stems, leaves, roots, buds and the like of the plants are cut (called cutting in gardening), or are inserted into soil, sand or are soaked in water, and the plants can be planted after rooting, so that the plants become independent new plants. The temperature suitable for growth of the calotropis gigantea is 20-35 ℃, and the created noodles are easy to be infected in the cutting process at the temperature, so that the phenomena of no rooting or even rotting root, prolonged rooting time and low survival rate occur. Therefore, a method for improving the rooting rate and the survival rate of cutting at a proper growth temperature of the cantaloupe is needed.
Disclosure of Invention
The invention aims to provide a cutting nutrient and a cutting method for Calotropis gigantea, which can obviously improve root length, rooting rate, nursery-out rate and survival rate of Calotropis gigantea.
In order to achieve the above object, the present invention provides the following technical solutions:
The invention provides a cutting nutrient for calotropis gigantea, which comprises the following components in parts by weight: 5-15 parts of salicylic acid, 10-20 parts of rice vinegar, 8-12 parts of dipotassium hydrogen phosphate and 3-9 parts of microbial agent.
Preferably, the microbial agent comprises: the weight ratio of the pseudomonas N21 to the candida FYAT to the bacillus subtilis B9601 to the Y2 is (1-3) to (1-3).
More preferably, the microbial agent has a bacterial count of more than 10 9/g.
The invention also provides a cutting method of the cantaloupe, which comprises the following steps: selecting 1-3 year-old cantaloupe, cutting main stems into stem segments with inclined openings at the lower ends of 5-13cm, soaking the lower ends of the stem segments in the cantaloupe cutting nutrient, and inserting the stem segments into a cutting matrix for cultivation.
Preferably, the stem segment comprises 1-3 axillary buds and 1-3 leaves.
Preferably, the dilution factor of the cutting nutrient for the Calotropis gigantea is 200-500.
Preferably, the soaking time is 10-20min.
Preferably, the cutting medium comprises: coconut chaff, perlite and peat soil; the mass ratio of the coconut coir, the perlite and the peat soil is (8-15): 3-8): 2-5; .
Preferably, the water content of the cutting matrix is 50% -60%; the cultivation temperature of the cuttage is 20-35 ℃.
The invention also provides application of the cutting nutrient for the cantaloupe or the cutting method for the cantaloupe in breeding of the cantaloupe.
Compared with the prior art, the invention has the following beneficial effects:
The invention provides a cutting nutrient for calotropis gigantea, which comprises the following components: salicylic acid, rice vinegar, dipotassium hydrogen phosphate and microbial agents, wherein the salicylic acid not only promotes rooting and can strengthen roots, but also can prevent rotten roots; the acetic acid contained in the rice vinegar has the effects of sterilizing and disinfecting, and simultaneously can provide nutrition components for the cutting wound of the cantaloupe and promote rooting; dipotassium hydrogen phosphate can improve plant stress resistance and adapt to the capability of external adverse environmental conditions; the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in a matrix, and achieves the effects of promoting rooting and improving survival rate. Experimental results show that after the cutting wound of the cantaloupe is soaked in the cantaloupe cutting nutrient before cutting, the root length, rooting rate, nursery yield and survival rate of the cantaloupe can be remarkably improved.
Detailed Description
The invention provides a cutting nutrient for calotropis gigantea, which comprises the following components in parts by weight: 5-15 parts of salicylic acid, 10-20 parts of rice vinegar, 8-12 parts of dipotassium hydrogen phosphate and 3-9 parts of microbial agent.
The cutting nutrient for the cantaloupe disclosed by the invention more preferably comprises the following components in parts by weight: 10 parts of salicylic acid, 15 parts of rice vinegar, 10 parts of dipotassium hydrogen phosphate and 6 parts of microbial agent.
In the present invention, the microbial agent preferably comprises: the weight ratio of the pseudomonas N21 to the candida FYAT to the bacillus subtilis B9601-Y2 is preferably (1-3): 1-3, more preferably 2:2:2; the number of bacteria in the microbial agent is preferably more than 10 9/g. The Pseudomonas N21 has a preservation number of CCTCCNO: M2016180, and is purchased from China center for type culture Collection; the candida FYAT1104 has a preservation number of CGMCC No.4761 and is purchased from the China general microbiological culture Collection center; the bacillus subtilis B9601-Y2 has a collection number of CGMCC No.0954 and is purchased from the China general microbiological culture Collection center (China Committee for culture Collection). The metabolite rhamnose and the metabolite arabitol of candida FYAT1104 in the cutting process of the pseudomonas N21 can be used as a surfactant, so that components in the nutrient can be fixed on cutting wounds of the cantaloupe, and the action time is prolonged to achieve the effects of promoting cutting rooting and improving the survival rate; the bacillus subtilis B9601-Y2 can effectively promote plant growth and rooting, has a good control effect on a plurality of soil-borne plant pathogens, and has good stress resistance. According to the invention, the substrate is not required to be sterilized in the cutting process, and the microbial agent has a synergistic effect among the pseudomonas N21, the candida FYAT and the bacillus subtilis B9601-Y2, and the addition of the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in the substrate, and achieves the effects of promoting rooting and improving survival rate.
In the invention, the salicylic acid not only promotes rooting and can strengthen the root, but also can prevent root rot; the acetic acid contained in the rice vinegar can sterilize and disinfect, and simultaneously can provide nutrition for the cutting wound of the cantaloupe so as to promote rooting; the dipotassium hydrogen phosphate can improve plant stress resistance and adapt to the capability of external adverse environmental conditions; the microbial agent can improve the stress resistance and disease resistance of the Calotropis gigantea plant, can avoid root rot caused by invasion of microorganisms in a matrix, and achieves the effects of promoting rooting and improving survival rate.
The preparation method of the cutting nutrient for the Calotropis gigantea is not particularly limited and can be achieved by adopting a conventional method in the field. The cutting nutrient for the Calotropis gigantea comprises a solid component and a liquid component, so that the cutting nutrient is prepared before cutting. As an embodiment, salicylic acid, rice vinegar, dipotassium hydrogen phosphate and microbial agent are mixed.
The invention also provides a cutting method of the cantaloupe, which comprises the following steps: selecting 1-3 year-old cantaloupe, cutting main stems into stem segments with inclined openings at the lower ends of 5-13cm, soaking the lower ends of the stem segments in the cantaloupe cutting nutrient, and inserting the stem segments into a cutting matrix for cultivation.
In the invention, the calotropis gigantea preferably selects 2-year-old main stems, and the stem segments preferably comprise 1-3 axillary buds, 1-3 leaves, more preferably 2 axillary buds and 2 leaves; the dilution factor of the cutting nutrient for Calotropis gigantea is preferably 200-500, more preferably 300, and the solvent in the dilution process is water; the soaking time is preferably 10-20min, more preferably 15min.
In the present invention, the cutting medium preferably comprises: coconut chaff, perlite and peat soil; the mass ratio of the coconut coir, the perlite and the peat soil is preferably (8-15): (3-8): (2-5), more preferably 10:5:4; the moisture content of the cutting matrix is preferably 50% -60%, more preferably 55%; the cultivation temperature of the cuttage is preferably 20-35 ℃, more preferably 30 ℃. Too much and too little water content in the cutting medium is detrimental to rooting of the wound. The cuttage matrix has good porosity, air permeability and water retention, can ensure that the calotropis gigantea is not easy to rot at a higher temperature, is more beneficial to root system respiration after rooting, and is not easy to cause choking and death.
The invention also provides application of the cutting nutrient for the cantaloupe or the cutting method for the cantaloupe in breeding of the cantaloupe.
In the present invention, all raw material components are commercially available products well known to those skilled in the art unless specified otherwise.
The technical solutions of the present invention will be clearly and completely described in the following in connection with the embodiments of the present invention. It will be apparent that the described embodiments are only some, but not all, embodiments of the invention. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
Calotropis gigantea cutting nutrient
Accurately weighing 10g of salicylic acid, 15g of rice vinegar, 10g of dipotassium hydrogen phosphate, 21 g of pseudomonas N, FYAT g of candida FYAT g 1104 and 22 g of bacillus subtilis B9601-Y, and mixing.
Example 2
Calotropis gigantea cutting nutrient
Accurately weighing 5g of salicylic acid, 10g of rice vinegar, 8g of dipotassium hydrogen phosphate, 21 g of pseudomonas N, FYAT g of candida FYAT g of 1104 g and 9601-Y2 1g of bacillus subtilis, and mixing.
Example 3
Calotropis gigantea cutting nutrient
15G of salicylic acid, 20g of rice vinegar, 12g of dipotassium hydrogen phosphate, 21 g of pseudomonas N, FYAT g of candida FYAT g 1104 and 9601-Y2 3g of bacillus subtilis are accurately weighed and mixed.
Example 4
Cutting of Calotropis gigantea
(1) Selecting a 2-year-old Calotropis gigantea plant, and cutting a main stem into a stem section with a bevel at the lower end of 10cm, wherein the stem section contains 2 axillary buds and 2 leaves;
(2) Inserting the stem into the cutting nutrient of the Calotropis gigantea of the embodiment 1 diluted 300 times by water, and soaking for 15min;
(3) Putting coconut coir, perlite and peat soil serving as a cutting matrix into a seedling pot with the specification diameter of 10 multiplied by the height of 10 multiplied by the bottom diameter of 11 multiplied by 8.5cm according to the weight ratio of 10:5:4, and keeping the water content of the cutting matrix to be 55%;
(4) The soaked stem segments are inserted into a cutting matrix and then cultivated in an environment of 30 ℃.
Example 5
Cutting of Calotropis gigantea
(1) Selecting 1-year-old Calotropis gigantea plant, and cutting the main stem into stem segments with a bevel opening at the lower end of 5cm, wherein the stem segments comprise 1 axillary bud and 3 leaves;
(2) Inserting the stem into the cutting nutrient of the Calotropis gigantea of the embodiment 2 diluted 200 times by water, and soaking for 10min;
(3) Putting coconut coir, perlite and peat soil serving as a cutting matrix into a seedling pot with the specification diameter of 10 multiplied by the height of 10 multiplied by the bottom diameter of 11 multiplied by 8.5cm according to the weight ratio of 8:3:2, and keeping the water content of the cutting matrix to be 60%;
(4) The soaked stem segments are inserted into a cutting matrix and then cultivated in an environment of 20 ℃.
Example 6
Cutting of Calotropis gigantea
(1) Selecting a 3-year-old cantaloupe plant, and cutting a main stem into a stem section with a 13cm lower end bevel, wherein the stem section contains 3 axillary buds and 1 leaf;
(2) Inserting the stem into the cutting nutrient of the Calotropis gigantea of the embodiment 3 diluted 500 times by water, and soaking for 20min;
(3) Putting coconut coir, perlite and peat soil serving as a cutting matrix into a seedling pot with the specification diameter of 10 multiplied by the height of 10 multiplied by the bottom diameter of 11 multiplied by 8.5cm according to the weight ratio of 15:8:5, and keeping the water content of the cutting matrix to be 50%;
(4) The soaked stem segments are inserted into a cutting matrix and then cultivated in an environment of 30 ℃.
Comparative example 1
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise microbial agents, the mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of salicylic acid to rice vinegar to dipotassium hydrogen phosphate is 2:3:2.
Comparative example 2
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise salicylic acid, the total mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of rice vinegar, dipotassium hydrogen phosphate, pseudomonas N21, candida FYAT1104 and bacillus subtilis B9601-Y2 is 15:10:2:2:2.
Comparative example 3
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise rice vinegar, the total mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of salicylic acid, dipotassium hydrogen phosphate, pseudomonas N21, candida FYAT1104 and bacillus subtilis B9601-Y2 is 5:5:1:1:1.
Comparative example 4
The specific embodiment is the same as in example 4, except that the cutting nutrient for the cantaloupe does not comprise dipotassium hydrogen phosphate, the total mass of the cutting nutrient for the cantaloupe is 41g, and the mass ratio of salicylic acid, rice vinegar, pseudomonas N21, candida FYAT1104 and bacillus subtilis B9601-Y2 is 10:15:2:2:2.
Comparative example 5
The specific embodiment is the same as example 4, except that the cutting nutrient of Calotropis gigantea is replaced with water.
The raw cantaloupe plants referred to in examples 4-6 and comparative examples 1-5 were obtained from a cantaloupe seed planting base in Honghe county of Yunnan, and transplanted to a plateau forestry institute laboratory of China forestry science institute as a cantaloupe material to be subjected to cutting propagation by seed propagation seedlings. The raw cantaloupe plants of the examples 4-6 and the comparative examples 1-5 are subjected to cuttage on the day 08 and 15 of 2020, the diameters and wound areas of cuttage stem segments are not obviously different, the cuttage number of each example or comparative example is 100, and the management after cuttage is carried out according to a conventional method in the field.
Experimental example 1
The cuttings of examples 4 to 6 and comparative examples 1 to 5 were examined after 10 days of cutting, and the average root number, average root length, nursery rate and survival rate after 3 months of transplanting were measured.
Root number means the number of white roots (roots) protruding from the base of the slip; average root number = root number sum of root stem segments/root stem segment number 100%; root length means the length (cm) of the longest root system measured from the root of the slip; average root length = root length sum of root stem segments/root stem segment number 100%; rooting rate = rooting stem/100 x 100%; nursery rate = suitable transplanted stem segments/100 x 100%; survival = surviving stem segments after transplanting/stem segments suitable for transplanting 100%. The specific results are shown in Table 1.
TABLE 1 Effect of different treatments on Calotropis gigantea cutting parameters
As shown in Table 1, the cutting method of Calotropis gigantea of the present invention was significantly improved in average root number, average root length, rooting rate, nursery rate and survival rate compared with comparative examples 1 to 5 after cutting for 10 days.
The foregoing is merely a preferred embodiment of the present invention and it should be noted that modifications and adaptations to those skilled in the art may be made without departing from the principles of the present invention, which are intended to be comprehended within the scope of the present invention.

Claims (8)

1. The cutting nutrient for the cantaloupe is characterized by comprising the following components in parts by weight: 5-15 parts of salicylic acid, 10-20 parts of rice vinegar, 8-12 parts of dipotassium hydrogen phosphate and 3-9 parts of microbial agent;
The microbial agent comprises the following components: the weight ratio of the pseudomonas N21 to the candida FYAT to the bacillus subtilis B9601 to the Y2 is (1-3) to (1-3);
The number of bacteria in the microbial agent is more than 10 9 /g.
2. The cutting method of the cantaloupe is characterized by comprising the following steps of:
Cutting 1-3 year-old Calotropis gigantea main stem into stem segments with inclined openings at the lower ends of 5-13 cm, soaking the lower ends of the stem segments in the Calotropis gigantea cutting nutrient according to claim 1, and inserting the stem segments into a cutting matrix for cultivation.
3. The cutting method of Calotropis gigantea according to claim 2, wherein the stem section comprises 1-3 axillary buds and 1-3 leaves.
4. The cutting method of the cantaloupe according to claim 2, wherein the dilution factor of the cantaloupe cutting nutrient is 200-500.
5. The cutting method of Calotropis gigantea according to claim 2, wherein the soaking time is 10-20 min.
6. The cutting method of calotropis gigantea according to claim 2, wherein the cutting medium comprises: coconut chaff, perlite and peat soil; the mass ratio of the coconut coir, the perlite and the peat soil is (8-15)/(3-8)/(2-5).
7. The cutting method of Calotropis gigantea according to claim 2, wherein the water content of the cutting medium is 50% -60%; the cultivation temperature of the cuttage is 20-35 ℃.
8. Use of the cantaloupe cutting nutrient of claim 1 or the cantaloupe cutting method of any one of claims 2-7 in cantaloupe breeding.
CN202211277576.3A 2022-10-19 2022-10-19 Calotropis gigantea cutting nutrient and cutting method Active CN115606593B (en)

Priority Applications (1)

Application Number Priority Date Filing Date Title
CN202211277576.3A CN115606593B (en) 2022-10-19 2022-10-19 Calotropis gigantea cutting nutrient and cutting method

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
CN202211277576.3A CN115606593B (en) 2022-10-19 2022-10-19 Calotropis gigantea cutting nutrient and cutting method

Publications (2)

Publication Number Publication Date
CN115606593A CN115606593A (en) 2023-01-17
CN115606593B true CN115606593B (en) 2024-04-19

Family

ID=84862510

Family Applications (1)

Application Number Title Priority Date Filing Date
CN202211277576.3A Active CN115606593B (en) 2022-10-19 2022-10-19 Calotropis gigantea cutting nutrient and cutting method

Country Status (1)

Country Link
CN (1) CN115606593B (en)

Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1554744A (en) * 2003-12-28 2004-12-15 何月秋 Bacillus subtilis strain
CN102533571A (en) * 2011-12-30 2012-07-04 安徽丰原发酵技术工程研究有限公司 Tropic candida sp. mutant strain and application thereof
CN106119148A (en) * 2016-06-07 2016-11-16 江苏联海生物科技有限公司 One pseudomonas and application thereof
CN107750687A (en) * 2017-11-07 2018-03-06 广西壮族自治区林业科学研究院 A kind of container seedling culture method bred using Calotropis gigantea branch cutting

Patent Citations (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1554744A (en) * 2003-12-28 2004-12-15 何月秋 Bacillus subtilis strain
CN102533571A (en) * 2011-12-30 2012-07-04 安徽丰原发酵技术工程研究有限公司 Tropic candida sp. mutant strain and application thereof
CN106119148A (en) * 2016-06-07 2016-11-16 江苏联海生物科技有限公司 One pseudomonas and application thereof
CN107750687A (en) * 2017-11-07 2018-03-06 广西壮族自治区林业科学研究院 A kind of container seedling culture method bred using Calotropis gigantea branch cutting

Also Published As

Publication number Publication date
CN115606593A (en) 2023-01-17

Similar Documents

Publication Publication Date Title
CN112889672B (en) Cultivation method for high-quality and high-yield bletilla striata seedlings
CN103875515A (en) Ex-vitro rooting method for tissue culture blueberry seedlings
CN106332716A (en) Camphor tree cuttage method
CN110301337A (en) A kind of rubber tree cuttage and seedling culture method
CN112753393B (en) Broussonetia papyrifera root propagation method
CN108551962A (en) A kind of method of Ling Yaoxing and peach interplanting
CN105875412B (en) The implantation methods of organic selenium-rich sugariness gynostemma pentaphylla
CN110338048B (en) Soilless culture method for cherry tomatoes
JP2021185763A (en) Method for promoting seed germination of poorly-germinated plant
CN109496655B (en) Seed germination and seedling raising method for camellia azalea
CN110622716A (en) Tomato grafting and seedling raising method
CN115606593B (en) Calotropis gigantea cutting nutrient and cutting method
CN114532225B (en) Tissue culture rapid propagation and cultivation method for paphiopedilum delbrueckii
CN106922397B (en) Method for cultivating easily-branched poinsettia
CN109618697A (en) A kind of cutting medium and Yin Maodan cuttage breeding method for Yin Maodan breeding
KR101064947B1 (en) The mass producing method of regenerated plant from the leaf segment of calanthe discolor
CN105379471B (en) A kind of method of crape myrtle seed treatment prevention powdery mildew
CN110558130B (en) Cutting method of cauliflower
Sharma et al. Growing media and cow urine influence the seed germination and seedling growth of papaya (Carica papaya L.).
CN112772391A (en) Water culture method for oats
CN104642110A (en) Induction method for autotetraploids of pomegranates
CN111448985A (en) Tissue culture method of rosa tenuifolia
CN108575521A (en) A kind of method of Ling Yaoxing and red bayberry interplanting
CN115500259B (en) Method for overcoming pollinosis of pumpkin interspecific hybridization offspring
CN107047295A (en) A kind of sunflower tissue culture method

Legal Events

Date Code Title Description
PB01 Publication
PB01 Publication
SE01 Entry into force of request for substantive examination
SE01 Entry into force of request for substantive examination
GR01 Patent grant
GR01 Patent grant