CN1155695C - Process for preparing water-preserving granular stain of edible (medicinal)fungus - Google Patents
Process for preparing water-preserving granular stain of edible (medicinal)fungus Download PDFInfo
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- CN1155695C CN1155695C CNB011175893A CN01117589A CN1155695C CN 1155695 C CN1155695 C CN 1155695C CN B011175893 A CNB011175893 A CN B011175893A CN 01117589 A CN01117589 A CN 01117589A CN 1155695 C CN1155695 C CN 1155695C
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Abstract
The present invention relates to a method for preparing water-preserving granular strains of edible (medical) fungi, which comprises the technological steps: the preparation of wood-dust test tube strains of the edible fungi, liquid mycelium obtainment after enlargement and the cultivation of the prepared wood-dust test tube strains of the edible fungi, nutrition granule preparation, nutrition granule vaccination, etc. The method solves the problems of long cycles, high technical requirements, difficult mastery of mushroom farmers, easy cultivation failure, difficult preservation of traditional strains, inconsistent fungus ages and inconvenient operation of technology in the prior art. The method has the advantages of fluent technological processes, strong water absorption, favorable water retention effect, convenient storage and transportation, etc.; furthermore, the method is suitable for industrial production, and the mechanical strength of dried granules is high (up than 40N).
Description
The present invention relates to a kind of food (medicine) and use process for preparing water-preserving granular stain of edible (medicinal) fungus.
China begins the promotion and application of artificial culture edible mushrooms technology from the sixties; become one of main edible mushrooms exporting country in the world at present; the making of edible fungus species is the key link of cultivation technique; domestic edible fungus culturing is continued to use traditional production of hybrid seeds technology basically; because process cycle is long; the technical requirements height; the mushroom farming is difficult for grasping; cause the major cause of cultivation failure often; and traditional bacterial classification is difficult for preserving; cell age is inconsistent, uses inconvenient shortcoming also to hinder edible fungus culturing and realizes large-scale development.
The object of the present invention is to provide a kind of food (medicine) to use process for preparing water-preserving granular stain of edible (medicinal) fungus, adopt the bacterial classification of the alternative traditional technology production of edible mushrooms water preserving granular spawn of this explained hereafter, make bacterial classification be convenient to storage and transport, easy to use, can realize batch production production in batches.
Overall technology design of the present invention is:
Food (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, is made up of following process steps:
The preparation of a, edible mushrooms wood chip test tube kind
With wood chip or grass meal or crop material powder: wheat bran or Semen Maydis powder: monose or disaccharides: CaSO4 or CaCO
3By weight for 78-88: 10-20: 1: 1 ratio is mixed evenly, with the said mixture material in mixture: water (w/v) is 1: the laggard test tube of the ratio mixing of 1.1-1.5 encapsulates high-temperature sterilization; Make edible mushrooms wood chip test tube kind with cultivating in the test tube substratum of original strain access after sterilization;
B, the above-mentioned edible mushrooms wood chip test tube kind that makes is obtained the liquid mycelium after enlarged culturing;
The preparation of c, nutritional granular
In grass meal: wood chip: wheat bran: Semen Maydis powder: water-holding agent: monose or disaccharides: CaSO4: tackiness agent: water (w/v) is sent into nodulizer for the ratio of 15-30: 15-30: 8-16: 3-8: 5-9: 0.5-3: 0.5-3: 40-70: 50-70 after being mixed evenly and is granulated, with the above-mentioned nutritional granular of making through high-temperature sterilization, drying, cooling;
D, inoculation
The liquid mycelium for preparing in the b step is inserted the nutritional granular of making in the c step, sprouted at normal temperatures 10 days, encapsulation.
Concrete processing step of the present invention and processing condition are:
At monose described in a and the c step or disaccharides is glucose or sucrose.
Select water-retaining resin for use at the water-holding agent described in the c step.
Water-retaining resin is polyacrylate or graft starch water-holding agent.
At the tackiness agent described in the c step is Starch Adhesive, the preparation method of this Starch Adhesive adds entry 180-360 part in reactor, be warming up to 55-70 ℃, drop into the slurry of 50-90 part starch furnishing, NaOH10-16 part of adding 30% is carried out gelatinization, is blended into 60-65 ℃ hot water then, and its volume is 1-3 a times of above-mentioned all raw material summations, dilute, add borax and be stirred to translucent for 13 parts.
In the high-temperature sterilization condition described in a step is 1.0-1.4kg/cm
2, the time is 0.5-1 hour.
In the condition of the high-temperature sterilization described in the c step is that 0.1MPa, 120 ℃, time are 1 hour; Drying conditions is that 40-70 ℃, time are 0.5 hour; The terminal temperature of cooling is 20-25 ℃.
All need pulverize the back at the grass meal described in the c step, wood chip, wheat bran and cross 20 mesh sieves, Semen Maydis powder is crossed the 40-60 mesh sieve, and water-holding agent is crossed the 20-60 mesh sieve.
At the nutritional granular diameter described in the c step is the cylindrical particle of 5-20mm, long 10-20mm.
The enlarged culturing described in the b step comprise by edible mushrooms wood chip test tube kind to liquid fermenting shake bottle the one-level enlarged culturing, shake bottle to the secondary enlarged culturing of fermentor tank by liquid fermenting; Wherein wood chip test tube kind culture condition is 20-28 ℃, time is 10-25 days, the processing condition of one-level enlarged culturing are 23-28 ℃, 150-200rpm shaking culture 24-72 hour, the processing condition of secondary enlarged culturing are air flow 1: 1 (v/v), under 23-28 ℃ temperature aeration-agitation 48-96 hour.
Substantive distinguishing features and technical progress that the present invention is obtained are:
Technological process smoothness of the present invention is particularly suitable for big batch production, granular bacteria strain intensity height, and dried particulate physical strength can reach more than the 40N, and the particle suction force is strong, and water conservation is effective, and long quality-guarantee period is convenient to storage and transportation in the production process.
Below in conjunction with embodiment the present invention is described further:
The preparation of a, edible mushrooms wood chip test tube kind
With wood chip: wheat bran: sucrose: CaSO4 is 80: 15: 1 by weight: 1 ratio is mixed evenly, with the said mixture material in mixture: water (w/v) is the test tube encapsulation of packing into behind 1: 1.2 the ratio mixing, high-temperature sterilization (1.1kg/cm
2, the time is 1 hour); Make edible mushrooms wood chip test tube kind with cultivating in the test tube substratum after the original strain access sterilization;
B, the above-mentioned edible mushrooms wood chip test tube kind that makes is obtained the liquid mycelium after enlarged culturing;
The preparation of c, nutritional granular
In grass meal: wood chip: wheat bran: Semen Maydis powder: water-holding agent: sucrose: CaSO4: tackiness agent: water (w/v) is 20: 20: 10: 5: 7: 2: send into nodulizer after 2: 50: 60 ratio is mixed evenly and granulate, (drying conditions is that 60 ℃, time are 0.5 hour through high-temperature sterilization (0.1MPa, 120 ℃, time are 1 hour), dry, cooling with the above-mentioned nutritional granular of making; The terminal temperature of cooling is 25 ℃);
D, inoculation
The liquid mycelium for preparing in the b step is inserted the nutritional granular of making in the c step, sprouted at normal temperatures 10 days, encapsulation.
Water-holding agent is selected water-retaining resin for use.Water-retaining resin is a sodium polyacrylate
At the tackiness agent described in the c step is Starch Adhesive, the preparation method of this Starch Adhesive adds 200 parts of entry in reactor, be warming up to 60 ℃, drop into the slurry of 70 parts of starch furnishings, NaOH15 part of adding 30% is carried out gelatinization, is blended into 65 ℃ hot water then, and its volume is 2 times of above-mentioned all raw material summations, dilute, add borax and be stirred to translucent for 13 parts.
Grass meal, wood chip, wheat bran all need be pulverized the back and cross 20 mesh sieves, and Semen Maydis powder is crossed 60 mesh sieves, and water-holding agent is crossed 30 mesh sieves.
Nutritional granular diameter described in the c step is 7mm, long 15mm cylindrical particle.
The enlarged culturing described in the b step comprise by edible mushrooms wood chip test tube kind to liquid fermenting shake a bottle one-level enlarged culturing, by liquid fermenting shake the bottle to the secondary enlarged culturing of fermentor tank, mycelial preparation; Wherein wood chip test tube kind culture condition is 25 ℃, time is 15 days, and the processing condition of one-level enlarged culturing are 26 ℃, 180rpm shaking culture 48 hours, the processing condition of secondary enlarged culturing are air flow 1: 1 (v/v), and aeration-agitation is 72 hours under 26 ℃ temperature.
Claims (10)
1, food (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that it is made up of following process steps:
The preparation of a, edible mushrooms wood chip test tube kind
With wood chip or grass meal or crop material powder: wheat bran or Semen Maydis powder: monose or disaccharides: CaSO4 or CaCO
3By weight for 78-88: 10-20: 1: 1 ratio is mixed evenly, with the said mixture material in mixture: water (w/v) is 1: the laggard test tube of the ratio mixing of 1.1-1.5 encapsulates high-temperature sterilization; Make edible mushrooms wood chip test tube kind with cultivating in the test tube substratum of original strain access after sterilization;
B, the above-mentioned edible mushrooms wood chip test tube kind that makes is obtained the liquid mycelium after enlarged culturing;
The preparation of c, nutritional granular
In grass meal: wood chip: wheat bran: Semen Maydis powder: water-holding agent: monose or disaccharides: CaSO4: tackiness agent: water (w/v) is sent into nodulizer for the ratio of 15-30: 15-30: 8-16: 3-8: 5-9: 0.5-3: 0.5-3: 40-70: 50-70 after being mixed evenly and is granulated, with the above-mentioned nutritional granular of making through high-temperature sterilization, drying, cooling;
D, inoculation
The liquid mycelium for preparing in the b step is inserted the nutritional granular of making in the c step, sprouted at normal temperatures 10 days, encapsulation.
2, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that at monose described in a and the c step or disaccharides be glucose or sucrose.
3, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that selecting water-retaining resin for use at the water-holding agent described in the c step.
4, food according to claim 3 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that described water-retaining resin is polyacrylate or graft starch water-holding agent.
5, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that at the tackiness agent described in the c step be Starch Adhesive, the preparation method of this Starch Adhesive adds entry 180-360 part in reactor, be warming up to 55-70 ℃, drop into the slurry of 50-90 part starch furnishing, NaOH10-16 part of adding 30% is carried out gelatinization, be blended into 60-65 ℃ hot water then, its volume is 1-3 a times of above-mentioned all raw material summations, dilute, add borax and be stirred to translucent for 13 parts.
6, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that in the high-temperature sterilization condition described in a step be 1.0-1.4kg/cm
2, the time is 0.5-1 hour.
7, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, and the condition at the high-temperature sterilization described in the c step of it is characterized in that is that 0.1MPa, 120 ℃, time are 1 hour; Drying conditions is that 40-70 ℃, time are 0.5 hour; The terminal temperature of cooling is 20-25 ℃.
8, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that all need pulverize the back at the grass meal described in the c step, wood chip, wheat bran crosses 20 mesh sieves, and Semen Maydis powder is crossed the 40-60 mesh sieve, and water-holding agent is crossed the 20-60 mesh sieve.
9, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that at the nutritional granular diameter described in the c step be the cylindrical particle of 5-20mm, long 10-20mm.
10, food according to claim 1 (medicine) is used process for preparing water-preserving granular stain of edible (medicinal) fungus, it is characterized in that the enlarged culturing described in the b step comprise by edible mushrooms wood chip test tube kind to liquid fermenting shake bottle the one-level enlarged culturing, shake bottle to the secondary enlarged culturing of fermentor tank by liquid fermenting; Wherein the culture condition of wood chip test tube kind is 20-28 ℃, time is 10-25 days, the processing condition of one-level enlarged culturing are 23-28 ℃, 150-200rpm shaking culture 24-72 hour, the processing condition of secondary enlarged culturing are air flow 1: 1 (v/v), under 23-28 ℃ temperature aeration-agitation 48-96 hour.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CNB011175893A CN1155695C (en) | 2001-04-27 | 2001-04-27 | Process for preparing water-preserving granular stain of edible (medicinal)fungus |
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| CNB011175893A CN1155695C (en) | 2001-04-27 | 2001-04-27 | Process for preparing water-preserving granular stain of edible (medicinal)fungus |
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| CN1316512A CN1316512A (en) | 2001-10-10 |
| CN1155695C true CN1155695C (en) | 2004-06-30 |
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| CNB011175893A Expired - Fee Related CN1155695C (en) | 2001-04-27 | 2001-04-27 | Process for preparing water-preserving granular stain of edible (medicinal)fungus |
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Families Citing this family (7)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102630485B (en) * | 2012-04-16 | 2013-08-21 | 何寒 | Method for cultivating flammulina velutipes through taking water-retaining agent solidified nutrient solution as culture medium |
| CN103011963B (en) * | 2012-12-18 | 2014-09-24 | 成都榕珍菌业有限公司 | First-level strain preservation culture medium for Pleurotus eryngii and preparation method of same |
| CN104109023A (en) * | 2014-07-22 | 2014-10-22 | 肥东县丰宝种养殖有限责任公司 | Flammulina velutipes culture medium containing paper pulp and preparation method thereof |
| CN104355909A (en) * | 2014-11-26 | 2015-02-18 | 天津市维益生物科技有限责任公司 | Culture medium for edible mushroom and preparation method of culture medium |
| CN106386169A (en) * | 2016-08-31 | 2017-02-15 | 佛山市高明区生产力促进中心 | Planting method for freshly growing mushroom |
| CN107142221A (en) * | 2017-06-26 | 2017-09-08 | 安庆师范大学 | A kind of preparation method and application of edible and medical fungi composite water conservation granular bacteria strain |
| CN109042073B (en) * | 2018-07-11 | 2021-04-16 | 鹰点（厦门）供应链管理有限公司 | Special biomass particle for edible fungi and preparation method thereof |
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