CN115521179A - Composition agent for plant continuous cropping diseases and application method thereof - Google Patents
Composition agent for plant continuous cropping diseases and application method thereof Download PDFInfo
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- CN115521179A CN115521179A CN202211340462.9A CN202211340462A CN115521179A CN 115521179 A CN115521179 A CN 115521179A CN 202211340462 A CN202211340462 A CN 202211340462A CN 115521179 A CN115521179 A CN 115521179A
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- 201000010099 disease Diseases 0.000 title claims abstract description 43
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 title claims abstract description 43
- 238000009335 monocropping Methods 0.000 title claims abstract description 29
- 239000000203 mixture Substances 0.000 title claims abstract description 23
- 238000000034 method Methods 0.000 title claims abstract description 15
- 241000223259 Trichoderma Species 0.000 claims abstract description 25
- 239000003795 chemical substances by application Substances 0.000 claims abstract description 25
- 150000001413 amino acids Chemical class 0.000 claims abstract description 18
- 229910052761 rare earth metal Inorganic materials 0.000 claims abstract description 15
- 229910052747 lanthanoid Inorganic materials 0.000 claims abstract description 14
- 150000002602 lanthanoids Chemical class 0.000 claims abstract description 14
- 150000002910 rare earth metals Chemical class 0.000 claims abstract description 13
- VEXZGXHMUGYJMC-UHFFFAOYSA-M Chloride anion Chemical compound [Cl-] VEXZGXHMUGYJMC-UHFFFAOYSA-M 0.000 claims abstract description 10
- 239000011573 trace mineral Substances 0.000 claims abstract description 9
- 235000013619 trace mineral Nutrition 0.000 claims abstract description 9
- 239000007788 liquid Substances 0.000 claims abstract description 7
- 229910001404 rare earth metal oxide Inorganic materials 0.000 claims abstract description 7
- 239000002994 raw material Substances 0.000 claims abstract description 4
- 241000223260 Trichoderma harzianum Species 0.000 claims description 15
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 10
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 6
- MRELNEQAGSRDBK-UHFFFAOYSA-N lanthanum(3+);oxygen(2-) Chemical compound [O-2].[O-2].[O-2].[La+3].[La+3] MRELNEQAGSRDBK-UHFFFAOYSA-N 0.000 claims description 6
- 239000003337 fertilizer Substances 0.000 claims description 5
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 5
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 5
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 5
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 5
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 claims description 4
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 claims description 4
- 238000005516 engineering process Methods 0.000 claims description 4
- 239000011574 phosphorus Substances 0.000 claims description 4
- 229910052698 phosphorus Inorganic materials 0.000 claims description 4
- 229910004664 Cerium(III) chloride Inorganic materials 0.000 claims description 3
- 241001460073 Trichoderma asperellum Species 0.000 claims description 3
- 241000894120 Trichoderma atroviride Species 0.000 claims description 3
- 239000000440 bentonite Substances 0.000 claims description 3
- 229910000278 bentonite Inorganic materials 0.000 claims description 3
- SVPXDRXYRYOSEX-UHFFFAOYSA-N bentoquatam Chemical compound O.O=[Si]=O.O=[Al]O[Al]=O SVPXDRXYRYOSEX-UHFFFAOYSA-N 0.000 claims description 3
- 229910000420 cerium oxide Inorganic materials 0.000 claims description 3
- VYLVYHXQOHJDJL-UHFFFAOYSA-K cerium trichloride Chemical group Cl[Ce](Cl)Cl VYLVYHXQOHJDJL-UHFFFAOYSA-K 0.000 claims description 3
- ICAKDTKJOYSXGC-UHFFFAOYSA-K lanthanum(iii) chloride Chemical compound Cl[La](Cl)Cl ICAKDTKJOYSXGC-UHFFFAOYSA-K 0.000 claims description 3
- 229910052757 nitrogen Inorganic materials 0.000 claims description 3
- BMMGVYCKOGBVEV-UHFFFAOYSA-N oxo(oxoceriooxy)cerium Chemical group [Ce]=O.O=[Ce]=O BMMGVYCKOGBVEV-UHFFFAOYSA-N 0.000 claims description 3
- GNSKLFRGEWLPPA-UHFFFAOYSA-M potassium dihydrogen phosphate Chemical compound [K+].OP(O)([O-])=O GNSKLFRGEWLPPA-UHFFFAOYSA-M 0.000 claims description 3
- LWIHDJKSTIGBAC-UHFFFAOYSA-K potassium phosphate Substances [K+].[K+].[K+].[O-]P([O-])([O-])=O LWIHDJKSTIGBAC-UHFFFAOYSA-K 0.000 claims description 3
- 229910001954 samarium oxide Inorganic materials 0.000 claims description 3
- 229940075630 samarium oxide Drugs 0.000 claims description 3
- BHXBZLPMVFUQBQ-UHFFFAOYSA-K samarium(iii) chloride Chemical compound Cl[Sm](Cl)Cl BHXBZLPMVFUQBQ-UHFFFAOYSA-K 0.000 claims description 3
- FKTOIHSPIPYAPE-UHFFFAOYSA-N samarium(iii) oxide Chemical compound [O-2].[O-2].[O-2].[Sm+3].[Sm+3] FKTOIHSPIPYAPE-UHFFFAOYSA-N 0.000 claims description 3
- 238000005507 spraying Methods 0.000 claims description 3
- 238000007598 dipping method Methods 0.000 claims description 2
- 230000002262 irrigation Effects 0.000 claims description 2
- 238000003973 irrigation Methods 0.000 claims description 2
- 238000002791 soaking Methods 0.000 claims description 2
- 241000196324 Embryophyta Species 0.000 abstract description 43
- 230000000694 effects Effects 0.000 abstract description 36
- 235000007688 Lycopersicon esculentum Nutrition 0.000 abstract description 26
- 230000012010 growth Effects 0.000 abstract description 19
- 230000007246 mechanism Effects 0.000 abstract description 9
- 230000003115 biocidal effect Effects 0.000 abstract description 5
- 230000002860 competitive effect Effects 0.000 abstract description 3
- 230000006698 induction Effects 0.000 abstract description 3
- 241000227653 Lycopersicon Species 0.000 abstract 4
- 102000004190 Enzymes Human genes 0.000 description 24
- 108090000790 Enzymes Proteins 0.000 description 24
- 229940088598 enzyme Drugs 0.000 description 24
- 240000003768 Solanum lycopersicum Species 0.000 description 22
- 239000002689 soil Substances 0.000 description 10
- 244000052616 bacterial pathogen Species 0.000 description 9
- 235000015097 nutrients Nutrition 0.000 description 9
- 239000000243 solution Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 8
- 239000002131 composite material Substances 0.000 description 7
- 108091005804 Peptidases Proteins 0.000 description 5
- 239000004365 Protease Substances 0.000 description 5
- 230000009471 action Effects 0.000 description 5
- 239000000126 substance Substances 0.000 description 5
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 5
- 108010059892 Cellulase Proteins 0.000 description 4
- 102000012286 Chitinases Human genes 0.000 description 4
- 108010022172 Chitinases Proteins 0.000 description 4
- XEEYBQQBJWHFJM-UHFFFAOYSA-N Iron Chemical compound [Fe] XEEYBQQBJWHFJM-UHFFFAOYSA-N 0.000 description 4
- 102100037486 Reverse transcriptase/ribonuclease H Human genes 0.000 description 4
- 229940106157 cellulase Drugs 0.000 description 4
- 230000002503 metabolic effect Effects 0.000 description 4
- 230000004060 metabolic process Effects 0.000 description 4
- 241000233622 Phytophthora infestans Species 0.000 description 3
- 239000003242 anti bacterial agent Substances 0.000 description 3
- 229940088710 antibiotic agent Drugs 0.000 description 3
- 239000003054 catalyst Substances 0.000 description 3
- 210000002421 cell wall Anatomy 0.000 description 3
- 239000003814 drug Substances 0.000 description 3
- 244000053095 fungal pathogen Species 0.000 description 3
- 230000035784 germination Effects 0.000 description 3
- -1 glucanase Proteins 0.000 description 3
- 230000006872 improvement Effects 0.000 description 3
- 238000004519 manufacturing process Methods 0.000 description 3
- 239000000463 material Substances 0.000 description 3
- UHPMCKVQTMMPCG-UHFFFAOYSA-N 5,8-dihydroxy-2-methoxy-6-methyl-7-(2-oxopropyl)naphthalene-1,4-dione Chemical compound CC1=C(CC(C)=O)C(O)=C2C(=O)C(OC)=CC(=O)C2=C1O UHPMCKVQTMMPCG-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 241000223218 Fusarium Species 0.000 description 2
- 206010061217 Infestation Diseases 0.000 description 2
- 241000700605 Viruses Species 0.000 description 2
- 239000012190 activator Substances 0.000 description 2
- 238000006243 chemical reaction Methods 0.000 description 2
- 235000013399 edible fruits Nutrition 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 230000001939 inductive effect Effects 0.000 description 2
- 229910052742 iron Inorganic materials 0.000 description 2
- 229910052751 metal Inorganic materials 0.000 description 2
- 229910021645 metal ion Inorganic materials 0.000 description 2
- 239000011259 mixed solution Substances 0.000 description 2
- 238000012986 modification Methods 0.000 description 2
- 230000004048 modification Effects 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 244000052769 pathogen Species 0.000 description 2
- 230000001717 pathogenic effect Effects 0.000 description 2
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- 239000008213 purified water Substances 0.000 description 2
- 238000011160 research Methods 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 238000004659 sterilization and disinfection Methods 0.000 description 2
- 239000013589 supplement Substances 0.000 description 2
- 238000010998 test method Methods 0.000 description 2
- 235000013311 vegetables Nutrition 0.000 description 2
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 1
- 108010084185 Cellulases Proteins 0.000 description 1
- 102000005575 Cellulases Human genes 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- JPVYNHNXODAKFH-UHFFFAOYSA-N Cu2+ Chemical compound [Cu+2] JPVYNHNXODAKFH-UHFFFAOYSA-N 0.000 description 1
- LEVWYRKDKASIDU-QWWZWVQMSA-N D-cystine Chemical compound OC(=O)[C@H](N)CSSC[C@@H](N)C(O)=O LEVWYRKDKASIDU-QWWZWVQMSA-N 0.000 description 1
- 206010059866 Drug resistance Diseases 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- 241000243785 Meloidogyne javanica Species 0.000 description 1
- 102000035195 Peptidases Human genes 0.000 description 1
- 241000233629 Phytophthora parasitica Species 0.000 description 1
- ZLMJMSJWJFRBEC-UHFFFAOYSA-N Potassium Chemical compound [K] ZLMJMSJWJFRBEC-UHFFFAOYSA-N 0.000 description 1
- 241000233639 Pythium Species 0.000 description 1
- 241001361634 Rhizoctonia Species 0.000 description 1
- 241001638069 Rigidoporus microporus Species 0.000 description 1
- XSQUKJJJFZCRTK-UHFFFAOYSA-N Urea Chemical compound NC(N)=O XSQUKJJJFZCRTK-UHFFFAOYSA-N 0.000 description 1
- 241000082085 Verticillium <Phyllachorales> Species 0.000 description 1
- XKMRRTOUMJRJIA-UHFFFAOYSA-N ammonia nh3 Chemical compound N.N XKMRRTOUMJRJIA-UHFFFAOYSA-N 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 230000000844 anti-bacterial effect Effects 0.000 description 1
- 239000003899 bactericide agent Substances 0.000 description 1
- 230000000443 biocontrol Effects 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000011575 calcium Substances 0.000 description 1
- 229910052791 calcium Inorganic materials 0.000 description 1
- 239000004202 carbamide Substances 0.000 description 1
- 239000013043 chemical agent Substances 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- 229910001431 copper ion Inorganic materials 0.000 description 1
- 229960003067 cystine Drugs 0.000 description 1
- 238000007405 data analysis Methods 0.000 description 1
- 230000007547 defect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 230000002950 deficient Effects 0.000 description 1
- 230000000593 degrading effect Effects 0.000 description 1
- 235000015872 dietary supplement Nutrition 0.000 description 1
- 230000002255 enzymatic effect Effects 0.000 description 1
- 238000006911 enzymatic reaction Methods 0.000 description 1
- 244000000004 fungal plant pathogen Species 0.000 description 1
- 230000009036 growth inhibition Effects 0.000 description 1
- 230000005923 long-lasting effect Effects 0.000 description 1
- 239000011777 magnesium Substances 0.000 description 1
- 239000002207 metabolite Substances 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000003032 phytopathogenic effect Effects 0.000 description 1
- 230000008635 plant growth Effects 0.000 description 1
- 239000011591 potassium Substances 0.000 description 1
- 229910052700 potassium Inorganic materials 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 230000008569 process Effects 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 230000001502 supplementing effect Effects 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 230000002194 synthesizing effect Effects 0.000 description 1
- 230000007723 transport mechanism Effects 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05B—PHOSPHATIC FERTILISERS
- C05B7/00—Fertilisers based essentially on alkali or ammonium orthophosphates
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01C—PLANTING; SOWING; FERTILISING
- A01C1/00—Apparatus, or methods of use thereof, for testing or treating seed, roots, or the like, prior to sowing or planting
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G13/00—Protecting plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01G—HORTICULTURE; CULTIVATION OF VEGETABLES, FLOWERS, RICE, FRUIT, VINES, HOPS OR SEAWEED; FORESTRY; WATERING
- A01G7/00—Botany in general
- A01G7/06—Treatment of growing trees or plants, e.g. for preventing decay of wood, for tingeing flowers or wood, for prolonging the life of plants
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N63/00—Biocides, pest repellants or attractants, or plant growth regulators containing microorganisms, viruses, microbial fungi, animals or substances produced by, or obtained from, microorganisms, viruses, microbial fungi or animals, e.g. enzymes or fermentates
- A01N63/30—Microbial fungi; Substances produced thereby or obtained therefrom
- A01N63/38—Trichoderma
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P21/00—Plant growth regulators
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G3/00—Mixtures of one or more fertilisers with additives not having a specially fertilising activity
- C05G3/60—Biocides or preservatives, e.g. disinfectants, pesticides or herbicides; Pest repellants or attractants
-
- C—CHEMISTRY; METALLURGY
- C05—FERTILISERS; MANUFACTURE THEREOF
- C05G—MIXTURES OF FERTILISERS COVERED INDIVIDUALLY BY DIFFERENT SUBCLASSES OF CLASS C05; MIXTURES OF ONE OR MORE FERTILISERS WITH MATERIALS NOT HAVING A SPECIFIC FERTILISING ACTIVITY, e.g. PESTICIDES, SOIL-CONDITIONERS, WETTING AGENTS; FERTILISERS CHARACTERISED BY THEIR FORM
- C05G5/00—Fertilisers characterised by their form
- C05G5/20—Liquid fertilisers
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
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- Life Sciences & Earth Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Chemical & Material Sciences (AREA)
- Environmental Sciences (AREA)
- Engineering & Computer Science (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Health & Medical Sciences (AREA)
- General Health & Medical Sciences (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Ecology (AREA)
- Forests & Forestry (AREA)
- Botany (AREA)
- Biodiversity & Conservation Biology (AREA)
- Microbiology (AREA)
- Mycology (AREA)
- Dentistry (AREA)
- Virology (AREA)
- Biotechnology (AREA)
- Toxicology (AREA)
- Soil Sciences (AREA)
- Agronomy & Crop Science (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of plant continuous cropping disease control, in particular to a composition agent for plant continuous cropping diseases and an application method thereof. The technical problem of the invention is that: the composition agent for the plant continuous cropping diseases and the application method thereof are provided, and the composition agent is prepared from the following raw materials in parts by weight: 6-10 parts of trichoderma; 20-35 parts of amino acid liquid; 0.1-0.5 part of trace elements; 2-5 parts of lanthanide rare earth chloride or rare earth oxide. The combined agent has the double effects of protection and treatment through mechanisms such as antibiotic action, competitive action, plant resistance induction and the like, the tomato emergence rate is up to 92.5 percent, the leaves of seedlings are dark green, the stems are thick and strong, and the seedling rate is up to 96 percent; during the growth period of the tomatoes, the tomato growth susceptibility is 0.4%; the growth of the plant root system is promoted and the comprehensive resistance of the plant is improved while the tomato root rot in the protected area is prevented and treated.
Description
Technical Field
The invention relates to the technical field of plant continuous cropping disease control, in particular to a composition agent for plant continuous cropping diseases and an application method thereof.
Background
During the continuous cultivation process of crops, soil-borne diseases, root diseases and the like can not be effectively prevented and controlled, so that the crops can be easily infected on seedling plants of the crops during planting, commonly known as: continuous cropping disease, continuous cropping disease or root rot disease. The concrete expression is as follows: the crops caused by soil organisms grow in the same place for a long time, and some pathogenic microorganisms such as late blight of tomato and fusarium are remained in the soil or plant residues for a large amount of reproduction, and the base number is increased year by year. When the seedlings are cultivated in continuous cropping, the seedlings are easy to be infected with diseases due to weak resistance. For example, root-knot nematodes multiply in soil year by year, destroy the root system of crops and influence the growth of crops. The roots of the crops are rotten, longitudinally split and undeveloped, and the normal physiological metabolism of the crops is influenced.
At present, trichoderma is used for preventing and treating root diseases and soil-borne diseases of plants such as vegetables, traditional Chinese medicinal materials, fruits, flowers and the like, and can solve the problem of continuous cropping caused by continuous cropping. The action mechanism of disease control is as follows: the antibiotic effect is as follows: trichoderma harzianum can secrete antibiotics and enzymes to inhibit the growth of pathogenic bacteria. The nutrition competition effect is as follows: currently, it is more obvious that the competition of iron and copper ions is only concerned, because such substances also reduce the germination and growth of pathogenic bacteria. Inducing the plants to generate resistance: after the plant is treated by trichoderma, the plant can induce and generate substances such as special ferment and the like, and further generate resistance to leaf diseases or virus diseases.
The research and application of trichoderma are rapidly developed, the problem that the large-scale popularization of the trichoderma in a biological disease control system is limited due to the fact that the metabolic enzyme yield and the enzyme activity of the trichoderma are not high when the trichoderma is applied as an agricultural medicament, and the mutual relation among the trichoderma, metabolites of the trichoderma, biocontrol bacteria and pathogenic bacteria is fully known, so that the technical problem that a new biological component combined control technology of the trichoderma is developed to improve the metabolic enzyme yield and the enzyme activity of the trichoderma is urgently needed to be solved by people in the field.
Disclosure of Invention
In order to overcome the defects that plant continuous cropping diseases cannot be effectively controlled and the yield and the enzyme activity of trichoderma metabolic enzyme are not high, the invention has the technical problems that: provides a composite agent aiming at plant continuous cropping diseases and an application method thereof.
The technical implementation scheme of the invention is as follows: a composition agent for plant continuous cropping diseases is prepared from the following raw materials in parts by weight: 6-10 parts of trichoderma; 20-35 parts of amino acid liquid; 0.1-0.5 part of trace elements; 2-5 parts of lanthanide rare earth chloride or rare earth oxide.
In a preferred embodiment of the invention, the trichoderma is trichoderma atroviride, trichoderma harzianum or trichoderma asperellum; preferably Trichoderma harzianum.
In a preferred embodiment of the invention, the amino acid solution is selected from the group consisting of MEM amino acid solutions manufactured by Wu Hanpu Nosai Life technologies, inc.
In a preferred embodiment of the invention, the lanthanide rare earth chloride is cerium trichloride, lanthanum trichloride or samarium trichloride in the same parts by weight; the lanthanide rare earth oxide is cerium oxide, lanthanum oxide or samarium oxide with the same weight part.
In a preferred embodiment of the invention, the trace element is prepared by uniformly mixing 0.4% of monopotassium phosphate and 0.1% of magnesium sulfate solution in a weight ratio of 1: 1.
A method for applying a composite agent for plant continuous cropping diseases comprises the step of using at least one of diatomite and bentonite as a carrier and applying the carrier and nitrogen and phosphorus fertilizers to root systems of various crops together, wherein the root systems are treated by adopting a seedbed, and seeds can be soaked, the roots can be dipped or the roots can be irrigated independently.
An application method of a composite agent for continuous cropping diseases of plants is characterized in that the composite agent can be applied to the leaf surfaces of crops after being hydrolyzed and diluted by 200-500 times and is subjected to leaf surface spraying treatment.
The invention has the following advantages: 1. the combined agent has the double effects of protection and treatment through mechanisms such as antibiotic action, competitive action, plant resistance induction and the like, the tomato emergence rate is up to 92.5 percent, the leaves of seedlings are dark green, the stems are thick and strong, and the seedling rate is up to 96 percent; during the growth period of the tomatoes, the tomato growth susceptibility is 0.4%; the growth of the plant root system is promoted and the comprehensive resistance of the plant is improved while the tomato root rot in the protected area is prevented and treated.
2. Under the microscopic level of enzyme activity determination, the enzyme yield in the metabolic process of the trichoderma harzianum is improved by the yield of chitinase, glucanase, cellulase, protease and the like generated by the trichoderma harzianum under the action of amino acid liquid and nutrient elements of trace elements, and lanthanide rare earth chloride is taken as a transportation sharp soldier of an enzyme activity activator, does not participate in reaction, but plays a role of a catalyst in a transportation mechanism, further improves the activity of the enzyme, and has a remarkable improvement effect on an antibiotic action mechanism.
Detailed Description
The following embodiments are further described in detail, but not intended to limit the scope of the present invention.
A composite agent for plant continuous cropping diseases is prepared from the following raw materials in parts by weight: 6-10 parts of trichoderma; 20-35 parts of amino acid liquid; 0.1-0.5 part of trace elements; 2-5 parts of lanthanide rare earth chloride or rare earth oxide.
In a preferred embodiment of the invention, the trichoderma is trichoderma atroviride, trichoderma harzianum or trichoderma asperellum; preferably Trichoderma harzianum.
Trichoderma bacteriostasis mechanisms include the production of antibiotics: studies have shown that trichoderma harzianum produces chitinases, glucanases, cellulases and proteases to break down the cell walls of phytopathogenic fungi to inhibit the growth, reproduction and infestation of pathogenic fungi.
And (3) nutrition competition: at present, the more obvious people are only in the aspect of competition of iron, copper and the like, because the substances also reduce the germination and growth of pathogenic bacteria.
Inducing the plants to generate resistance: after the plant is treated by trichoderma, the plant can induce and generate special substances such as enzyme and the like, and further generate resistance to leaf diseases or virus diseases.
In a preferred embodiment of the invention, the amino acid solution is selected from the group consisting of MEM amino acid solutions manufactured by Wu Hanpu Nosai Life technologies, inc.
The function of amino acid: the amino acid is promoter and catalyst for synthesizing various enzymes in microbe body, and plays an important role in microbe metabolism, especially the cystine in the amino acid plays a role in promoting the production of chitinase, cellulase, protease, etc. in trichoderma metabolic activity.
In a preferred embodiment of the invention, the lanthanide rare earth chloride is cerium trichloride, lanthanum trichloride or samarium trichloride in the same parts by weight; the lanthanide rare earth oxide is cerium oxide, lanthanum oxide or samarium oxide with the same weight part.
Rare earth elements: the general name of 17 lanthanide series metal elements is a group of metal elements with very high activity, rare earth elements are contained, and the material properties are completely changed. Under the agricultural reputation of "super active calcium", the efficacy of which is:
1. the water and the nutrients in the soil around the root system of the plant are enriched to form a nutrient source, so that the stress resistance of the plant is greatly improved.
2. The activity of the enzyme is activated, and the enzyme activity is improved by more than 40 percent.
3. Rare earth is a transport infantry of trace elements, in the aspect of microorganism: for example, most metal ions such as K +, mg2+ and the like are indispensable to enzymatic reaction, and the enzymatic activity can be obviously improved; in the plant aspect: if nutrient elements are deficient or unbalanced, the rare earth supplement nutrient is supplied after the rare earth is used, and the nutrient is transported to the plant to supplement by the plant due to the deficiency of the nutrient elements, so that the plant nutrient supplement has quick effect on supplementing medium and trace nutrient elements required by the plant.
In a preferred embodiment of the invention, the trace element is prepared by uniformly mixing 0.4% of monopotassium phosphate and 0.1% of magnesium sulfate solution in a weight ratio of 1: 1.
An application method of a composite agent for plant continuous cropping diseases, the composite agent is matched with at least one of diatomite or bentonite to be used as a carrier and applied to root systems of various crops together with nitrogen and phosphorus fertilizers, and seedbed treatment is adopted, or seed soaking, root dipping or root irrigation alone can be adopted; the composition agent can be applied to the leaf surfaces of crops after being hydrolyzed and diluted by 200-500 times, and the leaf surface spraying treatment is adopted.
The composition mainly utilizes the disease control or pathogen growth inhibition mechanism of trichoderma, and controls root diseases and soil-borne diseases of plants such as vegetables, traditional Chinese medicinal materials, fruits, flowers and the like; the root diseases comprise root rot, damping off, blight, southern blight and the like; the pathogenic bacteria of the soil-borne disease are tomato late blight bacteria, pythium, fusarium, rhizoctonia, verticillium and the like; can solve the problem of continuous cropping diseases caused by continuous cropping.
The product can avoid and reduce the dosage of chemical bactericides, reduce the pollution of chemical agents to the environment, reduce the drug resistance of pathogenic bacteria, further reduce the input cost of diseases, and is economic and environment-friendly; the product can last for more than 3 months when being used at the root, can last for 7-15 days when being sprayed at the leaf, reduces the medicine using times and labor input, and has long lasting period.
Test examples
In order to further verify the superior performance of the composition against plant continuous cropping diseases, the inventors have conducted a series of research experiments, which are limited by the text, and only illustrate the experimental data of individual examples, and those skilled in the art can understand that the following experiments can be repeated with other examples of the invention to obtain the same or similar experimental conclusions.
Soil samples are collected in a place with serious tomato root rot, and the soil of a plough layer is taken to pass through a 2mm sieve and is uniformly mixed for later use. Through analysis, the soil has the water content of 17.6 percent, the pH value of 6.2, the ammonia nitrogen content of 16.3mg/kg, the quick-acting phosphorus content of 332.6mg/kg and the quick-acting potassium content of 521.5mg/kg, and belongs to sandy loam.
Test example 1 Effect of the combination agent on the rate of emergence and the rate of emergence of tomato
Selecting the soil sample culture test, and setting 4 treatment groups in total, wherein the treatment groups are respectively as follows:
a: purified water control group;
b: trichoderma harzianum 8g/m 2 (ii) a Amino acid solution 30g/m 2 ；
C: trichoderma harzianum 8g/m 2 (ii) a Amino acid solution 30g/m 2 (ii) a 0.4% potassium dihydrogen phosphate and 0.1% magnesium sulfate mixed solution 0.3g/m 2 ；
D: trichoderma harzianum 8g/m 2 (ii) a Amino acid solution 30g/m 2 (ii) a 0.4% potassium dihydrogen phosphate and 0.1% magnesium sulfate mixed solution 0.3g/m 2 (ii) a Lanthanide rare earth chloride 3g/m 2 ；
The test method comprises the following steps:
1. in the seedling stage, 4 treatment groups are respectively soaked with tomato seeds according to the weight ratio of 1: 3,
2. when the tomatoes are planted, 2g of the fertilizer is applied to each hole of 4 treatment groups;
3. during the tomato growth period, 4 treatment groups were applied to the field separately with additional fertilizer every 100m 2 1-2kg of a combined agent, 4-6kg of diatomite and 2-3kg of urea are fully and evenly mixed and applied.
The percentage of each stage of diseased plant to the total plant is recorded respectively, wherein the emergence rate, the seedling rate and the growth susceptibility rate are recorded.
| Treatment group | The rate of emergence% | Percent of seedling formation | The growth susceptibility% |
| A | 63.5 | 66.7 | 42.3 |
| B | 74.4 | 70.5 | 17.6 |
| C | 82.6 | 86.3 | 9.2 |
| D | 92.5 | 96.0 | 0.4 |
Table 1 shows the effect of different compositions on the susceptibility of tomato at various growth stages
And (3) data analysis:
in a tomato cultivation test, the emergence rate of the tomatoes in the treatment group D is up to 92.5 percent, which is 29 percent higher than that of the tomatoes in a clear water control group, the leaves of the seedlings are dark green, the stems are strong, and the seedling rate is up to 96 percent; during tomato growth, the tomato growth susceptibility in the group treated with D was 0.4%.
The composition agent has double effects of protection and treatment through mechanisms such as antibiotic action, competitive action, plant resistance induction action and the like, promotes the growth of plant roots while preventing and treating the tomato root rot in a protected area, and improves the comprehensive resistance of plants.
Test example 2 Effect of the composition for controlling late blight of tomato
The soil sample culture test described above was selected in the same manner as in test example 1, and 4 treatment groups were provided in the same manner.
The test method comprises the following steps: and (3) dissolving the liquid medicine diluted by 300 times by using purified water, and irrigating the roots of the tomato crops naturally growing in the soil sample for the tomato late blight disease, wherein each plant is 500mL. And (5) separating soil-borne pathogenic fungi after the application of the pesticide for 5 days, and calculating the control effect.
(tomato late blight was isolated by Komada method; pathogen control effect was calculated by the following formula:
control effect% = (number of pathogenic bacteria in control group-number of pathogenic bacteria in combined agent treatment group)/number of pathogenic bacteria in control group 100%)
( On the basis of measuring reducing sugar by using a DNS method, measuring the activity of cellulase by using filter paper enzyme activity (FPA); protease activity was determined by Folin-phenol method; thereby indirectly measuring the enzyme yield and activity of the trichoderma. )
Table 2 shows the control effect of different compositions on late blight of tomato and the preservation rate data of the enzyme-producing activity of trichoderma fungus:
in the control effect test of tomato late blight, different combination agents have bacteriostasis and sterilization effects on tomato late blight, and compared with the combination agent of the treatment group D of B, C, the combination agent of the treatment group D has the strongest enzyme production activity of trichoderma, so that the bacteriostasis and sterilization effects are further improved.
On the microscopic level of enzyme activity determination, the enzyme yield in the metabolic process of the trichoderma harzianum is improved by the yields of chitinase, glucanase, cellulase, protease and the like generated by the trichoderma harzianum under the action of amino acid liquid and nutrient elements of trace elements, and lanthanide rare earth chloride is taken as a transport sharp soldier of an enzyme activity activator (metal ions K + and Mg < 2+ >) and does not participate in the reaction, but a transport mechanism plays the role of a catalyst, so that the activity of the enzyme is further improved; has obvious effect of improving the action mechanism of antibiotics.
The growth, reproduction and infestation of pathogenic fungi is inhibited by these high-yield and high-activity cell wall degrading enzymes breaking down the cell walls of the plant pathogenic fungi. Increase the germination rate of seeds, the survival rate of roots and seedlings and the growth resistance of plants.
Although the present invention has been described in detail hereinabove with reference to the general description, the specific embodiments and the experimental examples, it will be apparent to those skilled in the art that modifications and improvements can be made based on the present invention. Accordingly, such modifications and improvements are intended to be within the scope of the invention as claimed.
Claims (7)
1. A composition agent for plant continuous cropping diseases is characterized by comprising the following raw materials in parts by weight: 6-10 parts of trichoderma; 20-35 parts of amino acid liquid; 0.1-0.5 part of trace elements; 2-5 parts of lanthanide rare earth chloride or rare earth oxide.
2. The composition for controlling plant continuous cropping diseases according to claim 1, wherein said trichoderma is trichoderma atroviride, trichoderma harzianum or trichoderma asperellum; preferably Trichoderma harzianum.
3. The composition for plant continuous cropping diseases according to claim 1, wherein the amino acid solution is selected from MEM amino acid solution produced by Wu Hanpu Nosai Life technologies, inc.
4. The composition agent for the plant continuous cropping diseases according to claim 1, characterized in that the lanthanide rare earth chloride is cerium trichloride, lanthanum trichloride or samarium trichloride in the same parts by weight; the lanthanide rare earth oxide is cerium oxide, lanthanum oxide or samarium oxide with the same weight part.
5. The composition for controlling plant continuous cropping diseases according to claim 1, characterized in that the microelement is prepared by uniformly mixing 0.4% of monopotassium phosphate and 0.1% of magnesium sulfate solution in a weight ratio of 1: 1.
6. The method for applying the composition to the plant continuous cropping diseases according to any one of claims 1 to 5, characterized in that the composition is matched with at least one of diatomite or bentonite as a carrier and is applied to the root systems of various crops together with nitrogen and phosphorus fertilizers by adopting seedbed treatment, or seed soaking, root dipping or root irrigation alone.
7. The method for applying the composition to the plant continuous cropping disease according to any one of claims 1 to 5, characterized in that the composition can be applied to the leaf surfaces of crops after being hydrolyzed and diluted by 200 to 500 times and is subjected to leaf surface spraying treatment.
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