CN115518016A

CN115518016A - Antioxidant and antiaging composition containing flos Pyraricae extract, tea polyphenols and peptides, and its preparation method

Info

Publication number: CN115518016A
Application number: CN202211385888.6A
Authority: CN
Inventors: 康云香
Original assignee: Guangzhou Deyi Cosmetics Co ltd
Current assignee: Guangzhou Deyi Cosmetics Co ltd
Priority date: 2022-11-07
Filing date: 2022-11-07
Publication date: 2022-12-27
Anticipated expiration: 2042-11-07
Also published as: CN115518016B

Abstract

The invention relates to an antioxidant and anti-aging composition containing a common malus herb flower extract, tea polyphenol and peptides and a preparation method thereof, belonging to the technical field of cosmetics. The composition provided by the invention uses the extract of the American pear flower and the tea polyphenol to compound palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, oligopeptide-3 and dipeptide diaminobutyryl benzylamide diacetate, and has the effects of resisting oxidation and ageing; the invention also provides a preparation method of the composition, which has simple steps and is easy for industrial production.

Description

Antioxidant and antiaging composition containing flos Pyraricae extract, tea polyphenols and peptides, and its preparation method

Technical Field

The invention belongs to the technical field of cosmetics, and particularly relates to an antioxidant and anti-aging composition containing a common malpighia herb extract, tea polyphenol and peptides and a preparation method thereof.

Background

The skin undergoes changes of deterioration, aging and also a great change in its function. The factors of skin aging are divided into endogenous aging and exogenous aging, wherein the endogenous aging refers to a natural and inevitable aging process of the skin along with the aging process of a human body; exogenous aging mainly refers to photoaging, and the photochemical aging process of skin caused by ultraviolet rays (including environmental pollution and aging factors).

Endogenous aging causes skin aging in the same manner as other organs, and skin is affected by gravity, facial expression, hormonal changes and genetic factors, so that the skin is slowly and irreversibly degenerated, and endogenous aging mainly causes fine wrinkles and thinning of the epidermis.

Extrinsic aging is the combined result of all extrinsic factors that can lead to skin aging, such as lifestyle patterns, smoking, uv irradiation, and environmental pollution, which accumulate over time and is characterized by deep wrinkles, skin laxity, and pigmentation.

Photoaging is one of the major factors causing extrinsic skin aging, and it is currently believed that ultraviolet radiation is the major cause of photoaging. Endogenous aging skin changes are observed in the skin at non-light exposed sites, whereas photoaging-induced skin changes are significantly more pronounced at light exposed sites, such as the hands, face and neck, than in the aging manifestations caused by endogenous factors. There is a long-term accumulation of sunlight in daily life, which is unavoidable, and once photoaging begins, collagen fibers decrease, and the skin becomes flaccid and wrinkles appear. The dermal layer of the skin is a connective tissue containing a large amount of extracellular matrix (ECM) proteins mainly composed of collagen and elastin, and other components of the ECM, in addition to these fibrous proteins, are Proteoglycan (PG) and glycosaminoglycans (GAGs), which are amorphous, surrounding and embedding the fiber and cell matrix in the dermis. Collagen type I (COL 1) in dermal collagen fibers accounts for about 90% of collagen fibers, and gradually decreases as skin aging progresses due to low synthesis and high degradation, so that collagen fibers are broken, disordered, and agglomerated in photoaged skin. Studies have shown that photoaging causes a decrease in collagen stability by altering proline and strong proline residues in the dermis, and the density and morphology of collagen and elastin in the skin are markers for determining whether skin aging occurs.

In photoaging studies, it has been shown that ultraviolet radiation induces the expression of Matrix Metalloproteinases (MMPs) in human skin. MMPs function to degrade extracellular matrix proteins such as collagen, elastin, proteoglycans, and the like. MMP is a zinc-containing endopeptidase with a broad range of specific substrates that mediate the degradation of different components in the ECM. In the skin, keratinocytes of the epidermis and fibroblasts of the dermis secrete mainly MMP1 (collagenase 1), MMP1 degrades type I and type III collagen fibers into specific protein fragments at a single site in the triple helix, and other MMPs (e.g., gelatinases) hydrolyze these collagen fragments further, eventually leading to damage to the collagen-rich dermis.

With the age, the metabolism function of the skin is reduced, the function of an in-vivo antioxidant system is declined, the activity of antioxidant enzyme is continuously reduced, free radicals are excessively accumulated, clearance disorder occurs, and the accumulation of in-vivo oxidative irreversible damage is initiated; in addition, long-term over-irradiation by ultraviolet rays can also cause problems of oxidative stress, inflammatory reaction, telomere damage, collagen synthesis reduction and the like, leading to a series of aging damage and accelerating skin aging. The aging skin is mainly characterized by: loose skin, wrinkles, pigmentation, etc.

At present, most of anti-aging cosmetics on the market start from improving skin collagen, however, the collagen cosmetics are prepared by coating single collagen on the surface of skin, and the single anti-aging way is often poor in effect, so that the anti-aging effect is far from the expectation.

Disclosure of Invention

The invention relates to an antioxidant and anti-aging composition containing a American pear flower extract, tea polyphenol and peptides and a preparation method thereof, belonging to the technical field of cosmetics. The composition provided by the invention comprises the following raw materials in percentage by mass: western pear flower extract solution: 1-20%, tea polyphenol: 1-5%, palmitoyl tripeptide-5 solution: 1-10%, arginine/lysine polypeptide solution: 1-10%, acetyl hexapeptide-8 solution: 1-10%, carnosine: 0.01-0.5%, oligopeptide-3 solution: 0.1-5%, dipeptide diaminobutyrylbenzyl amide diacetate solution 0.1-5%, phenoxyethanol solution: 0.5-5%, butanediol: 1-30%, citric acid: 0.01-0.5%, xanthan gum: 0.01-0.5% and the balance of ionized water; the invention uses the extract of the American pear flower and the tea polyphenol to compound palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, oligopeptide-3 and dipeptide diaminobutyrylbenzyl amide diacetate, and has the functions of oxidation resistance and anti-aging; the invention also provides a preparation method of the composition, which has simple steps and is easy for industrial production.

The purpose of the invention can be realized by the following technical scheme:

an antioxidant and anti-aging composition containing a western pear flower extract, tea polyphenol and peptides comprises the following raw materials in percentage by mass:

american pear flower extract solution: 1-20%, tea polyphenol: 1-5%, palmitoyl tripeptide-5 solution: 1-10%, arginine/lysine polypeptide solution: 1-10%, acetyl hexapeptide-8 solution: 1-10%, carnosine: 0.01-0.5%, oligopeptide-3 solution: 0.1-5%, dipeptide diaminobutyrylbenzyl amide diacetate solution 0.1-5%, phenoxyethanol solution: 0.5-5%, butanediol: 1-30%, citric acid: 0.01-0.5%, xanthan gum: 0.01-0.5% and the balance of ionized water.

The American pear flower extract solution comprises 69.4% of water, 20% of butanediol, 10% of American pear flower extract and 0.6% of phenoxyethanol; further, the mass percent of the solution of the American pear flower extract is 1-10%.

The extract of Actinidia chinensis planch has antioxidant effect, and can scavenge hydroxyl free radical and inhibit lipid peroxidation. Pear flower is rich in nutrients such as vitamins, amino acids, minerals, etc., and its extract also contains a large amount of polyphenols such as arbutin, chlorogenic acid, etc., and flavonoids beneficial to skin. Researchers detected arbutin, glycosides, hydroquinone, flavonoids and the like in pear flowers of avocados. In another study, the investigators tested polyphenols in the pear flower extract and identified a phenolic 4-O-Z-coumaroyl rosin that was not reported in previous related studies. Researchers have shown that phenolics in pear flowers play a non-negligible role in the antioxidant capacity of pear flower extracts. According to the experimental results, the hydroquinone content in various polyphenol substances is the highest, and the antioxidant activity is the strongest. Another study indicated that the arbutin content in pear flower was relatively low, but the chlorogenic acid content was highest, compared to the buds, leaf buds, and young fruits.

Chlorogenic acid is an effective phenolic antioxidant with antioxidant capacity stronger than that of caffeic acid, p-hydroxybenzoic acid, ferulic acid, syringic acid, butylated hydroxyanisole and tocopherol. Chlorogenic acid has antioxidant effect because it contains a certain amount of R-OH groups, and can form hydrogen radicals with antioxidant effect to eliminate the activity of hydroxyl radicals and superoxide anions, etc., thereby protecting tissue from damage due to oxidation.

Arbutin is one of the important components in pear flower extracts known at present. The whitening effect and safety of use of arbutin on skin have been extensively studied and confirmed. Arbutin can effectively inhibit the generation of melanin of skin cells, and has certain antioxidant capacity. In addition, arbutin can be hydrolyzed under the influence of skin environment and flora to generate hydroquinone with good oxidation resistance, so that the oxidation resistance effect of arbutin is further improved. Arbutin and hydroquinone detected in the pear flower extract can contribute to the pear flower extract to have good whitening and oxidation resistance.

The extraction method of the American pear flower extract comprises the following operations:

chopping dry American pear flowers, selecting ethanol with the mass percent of 70% as an extraction solvent, soaking the American pear flowers in the extraction solvent for 0.5h, then carrying out reflux extraction, wherein the reflux extraction temperature is 70-80 ℃, the extraction frequency is 2 times, the extraction time is 2 hours each time, and the material-liquid ratio of the first reflux extraction is 1:15, the material-liquid ratio of the second reflux extraction is 1:10; filtering the extracts, and mixing the filtrates; and concentrating the filtrate under reduced pressure, and vacuum drying the concentrated solution to obtain the American pear flower extract.

Tea polyphenol is a natural antioxidant with strong oxidation resistance, has strong electron supply capacity, can be combined with free radicals in skin to be converted into inert substances, and stops chain reaction of the free radicals. Has strong scavenging function to free radicals.

The arginine/lysine polypeptide solution comprises 0.0024% of arginine/lysine polypeptide and 99.9976% of deionized water.

Arginine/lysine polypeptide: the venom is from marine mollusk conus, and has strong biological activity. When hunting, the conus can inject a small amount of venom (less than 50 mu L) into the prey through the needle-shaped tooth tongues and the venom gland ducts, and the venom contains a large amount of nerve active substances including various neurotoxin peptides, so that the prey can be paralyzed instantly and the predation purpose is achieved. Such neurotoxin peptides are collectively referred to as conopeptides. Conopeptides derived from conus have the advantages of multiple types, small molecules, strong effect, wide target spot, strong selectivity and the like, and can specifically block a voltage-gated sodium ion channel, so that the internal flow of sodium ions is blocked, and the action potential of muscles cannot be formed, thereby effectively preventing and reducing wrinkles. The arginine/lysine polypeptide synthesized by artificial scientific sequencing through a biological simulation synthesis technology can effectively relax muscles under the condition of not numbing muscle nerve conduction, thereby achieving the effect of removing wrinkles.

The palmitoyl tripeptide-5 solution comprises palmitoyl tripeptide-5.5%, glycerol 62% and deionized water 37.5%.

Palmitoyl tripeptide-5 can promote synthesis of collagen (type I, type II and type IV) and glucosamine in human fibroblasts, strengthen connective tissues, enhance skin compactness, promote cell activity, supplement collagen in skin, and improve skin elasticity.

The acetyl hexapeptide-8 solution comprises 0.1% of acetyl hexapeptide, 96.87% of deionized water, 3% of 1, 2-hexanediol and 0.03% of ethyl hexyl glycerol.

The acetyl hexapeptide-8 can block the transportation and release of acetylcholine of nerve cell bodies, weaken nerve transmission and further reduce muscle contraction to achieve the aim of reducing wrinkles.

The dipeptide diaminobutyrylbenzylamide diacetate solution comprises dipeptide diaminobutyrylbenzylamide diacetate 0.1%, deionized water 96.87%, 1, 2-hexanediol 3% and ethylhexylglycerin 0.03%.

Dipeptide diaminobutyrylbenzylamide diacetate is a synthetic peptide simulating snake venom serum, and can prevent acetylcholine nerve conduction, achieve the effect of relaxing muscles, and further improve dynamic wrinkles.

The oligopeptide-3 solution comprises 0.1% of oligopeptide-3, 5% of 1, 2-pentanediol, 11% of butanediol and 83.9% of deionized water.

Oligopeptide-3 is a peptide substance with biological activity, and can improve the microenvironment of cell growth, promote the synthesis of elastic fiber and collagen, make skin elastic and smooth and tender.

Carnosine has strong antioxidant and anti-glycation effects, prevents oxidative stress products and glycosylated end (AGE) products, simultaneously reduces the telomere damage and damage rate of fibroblasts, sustains collagen and elastic fibers, and maintains the density and elasticity of the skin.

The phenoxyethanol solution comprises phenoxyethanol 90% and ethylhexylglycerin 10%.

The composition of the present invention can be used in the form of an external preparation, a suppository or a sterile injection solution according to a conventional method. More specifically, the composition can be prepared by using diluents or excipients such as fillers, weighting agents, binders, wetting agents, disintegrants, and surfactants, which are generally used in formulation.

The composition of the present invention may include, in addition to the effective ingredients, common ingredients, for example, common adjuvants such as antioxidants, stabilizers, solubilizers, vitamins, pigments, and perfumes, and may further include a carrier.

The composition of the present invention may be prepared in any form commonly used in the art, for example, but not limited to, a solution, a suspension, an emulsion, a paste, a gel, a cream, a lotion, etc.; more specifically, it can be made into cosmetic water, nourishing cream, massage cream, essence, eye cream, cleansing foam, skin cleansing water or facial mask.

A method for preparing antioxidant and antiaging composition containing flos Pyraricae extract, tea polyphenols and peptides comprises the following steps:

step 1: adding deionized water and xanthan gum into vacuum homogenizing tank, homogenizing and stirring, heating to 80-85 deg.C, and keeping the temperature for 30min;

step 2: cooling to 60-65 deg.C, sequentially adding butanediol and phenoxyethanol into vacuum homogenizing tank, stirring and dispersing;

and step 3: cooling to 40-45 deg.C, sequentially adding flos Pyri Calleryanae extract, tea polyphenols, palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, carnosine, oligopeptide-3, dipeptide diaminobutyryl benzylamide diacetate, and citric acid into vacuum homogenizing tank, stirring and dispersing;

and 4, step 4: after stirring evenly, discharging after checking the qualified pH value.

The invention has the beneficial effects that:

1. the composition is compounded with palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, oligopeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate on the basis of a common pear flower extract and tea polyphenol, has the effects of resisting oxidation and aging, can eliminate in-vivo free radicals, promote the generation of collagen and delay and repair aging skin according to the aging reasons of skin internal and external sources, thereby achieving the purpose of resisting aging and being applied to the preparation of skin care products for resisting oxidation and aging;

2. a preparation method of a composition containing a American pear flower extract, tea polyphenol and peptides optimizes production process steps and parameters, and the steps are simple and easy to realize industrial production.

Detailed Description

The technical solutions in the embodiments of the present invention will be clearly and completely described below with reference to the embodiments of the present invention, and it is obvious that the described embodiments are only a part of the embodiments of the present invention, and not all of the embodiments. All other embodiments, which can be derived by a person skilled in the art from the embodiments given herein without making any creative effort, shall fall within the protection scope of the present invention.

Example 1

western pear flower extract solution: 1%, tea polyphenol: 1%, palmitoyl tripeptide-5 solution: 1%, arginine/lysine polypeptide solution: 1%, acetyl hexapeptide-8 solution: 1%, carnosine: 0.01%, oligopeptide-3 solution: 0.1 percent, 0.1 percent of dipeptide diaminobutyrylbenzyl amide diacetate solution, 0.1 percent of phenoxyethanol solution: 0.5%, butanediol: 1%, citric acid: 0.01%, xanthan gum: 0.01% and the balance of ionized water.

The solution of the American pear flower extract comprises 69.4% of water, 20% of butanediol, 10% of the American pear flower extract and 0.6% of phenoxyethanol.

The acetyl hexapeptide-8 solution comprises 0.1% of acetyl hexapeptide, 96.87% of deionized water, 3% of 1, 2-hexanediol and 0.03% of ethylhexyl glycerol.

An antioxidant and antiaging composition containing flos Pyraricae extract, tea polyphenols and peptides is prepared by the following preparation method:

step 1: adding deionized xanthan gum into vacuum homogenizing tank, homogenizing and stirring, heating to 80-85 deg.C, and keeping the temperature for 30min;

step 2: cooling to 60 ℃, sequentially putting the butanediol and phenoxyethanol solution into a vacuum homogenizing tank, and stirring and dispersing uniformly;

and step 3: cooling to 40 deg.C, sequentially adding flos Pyri Calleryanae extract solution, tea polyphenols, palmitoyl tripeptide-5 solution, arginine/lysine polypeptide solution, acetyl hexapeptide-8 solution, carnosine, oligopeptide-3 solution, dipeptide diaminobutyryl benzylamide diacetate solution, and citric acid into vacuum homogenizing tank, stirring and dispersing;

Example 2

american pear flower extract solution: 4%, tea polyphenol: 2%, palmitoyl tripeptide-5 solution: 4%, arginine/lysine polypeptide solution: 4%, acetyl hexapeptide-8 solution: 4%, carnosine: 0.2%, oligopeptide-3 solution: 2 percent of dipeptide diaminobutyrylbenzylamide diacetate solution, 2 percent of phenoxyethanol solution: 2%, butanediol: 7%, citric acid: 0.2%, xanthan gum: 0.2% and the balance of ionized water.

The solution of the extract of the American pear flower, the solution of arginine/lysine polypeptide, the solution of palmitoyl tripeptide-5, the solution of acetyl hexapeptide-8, the solution of dipeptide diaminobutyrylbenzylamide diacetate, the solution of oligopeptide-3 and the solution of phenoxyethanol are the same as those in example 1.

step 2: cooling to 62 ℃, sequentially putting the butanediol and phenoxyethanol solution into a vacuum homogenizing tank, and stirring and dispersing uniformly;

and step 3: cooling to 41 deg.C, sequentially adding flos Pyri Calleryanae extract solution, tea polyphenols, palmitoyl tripeptide-5 solution, arginine/lysine polypeptide solution, acetyl hexapeptide-8 solution, carnosine, oligopeptide-3 solution, dipeptide diaminobutyryl benzylamide diacetate solution, and citric acid into vacuum homogenizing tank, stirring and dispersing;

Example 3

western pear flower extract solution: 6%, tea polyphenols: 3%, palmitoyl tripeptide-5 solution: 6%, arginine/lysine polypeptide solution: 6%, acetyl hexapeptide-8 solution: 6%, carnosine: 0.3%, oligopeptide-3 solution: 3% of dipeptide diaminobutyrylbenzylamide diacetate solution, 3% of phenoxyethanol solution: 3% and butanediol: 15%, citric acid: 0.3%, xanthan gum: 0.3% and the balance of ionized water.

An antioxidant and antiaging composition containing extract of flos Pyrolae, tea polyphenols and peptides is prepared by the following preparation method:

step 2: cooling to 63 ℃, sequentially putting the butanediol and phenoxyethanol solution into a vacuum homogenizing tank, and stirring and dispersing uniformly;

and 3, step 3: cooling to 43 deg.C, sequentially adding flos Pyri Calleryanae extract solution, tea polyphenols, palmitoyl tripeptide-5 solution, arginine/lysine polypeptide solution, acetyl hexapeptide-8 solution, carnosine, oligopeptide-3 solution, dipeptide diaminobutyryl benzylamide diacetate solution, and citric acid into vacuum homogenizing tank, stirring and dispersing;

Example 4

american pear flower extract solution: 8%, tea polyphenols: 4%, palmitoyl tripeptide-5 solution: 8%, arginine/lysine polypeptide solution: 8%, acetyl hexapeptide-8 solution: 8%, carnosine: 0.4%, oligopeptide-3 solution: 4 percent of dipeptide diaminobutyrylbenzyl amide diacetate solution, 4 percent of phenoxyethanol solution: 4% and butanediol: 22%, citric acid: 0.4%, xanthan gum: 0.4% and the balance of ionized water.

The above-mentioned pear flower extract solution, arginine/lysine polypeptide solution, palmitoyl tripeptide-5 solution, acetyl hexapeptide-8 solution, dipeptide diaminobutyryl benzylamide diacetate solution, oligopeptide-3 solution, and phenoxyethanol solution are the same as those described in example 1.

and 2, step: cooling to 64 ℃, sequentially putting the butanediol and phenoxyethanol solution into a vacuum homogenizing tank, and stirring and dispersing uniformly;

and step 3: cooling to 44 deg.C, sequentially adding flos Pyri Calleryanae extract solution, tea polyphenols, palmitoyl tripeptide-5 solution, arginine/lysine polypeptide solution, acetyl hexapeptide-8 solution, carnosine, oligopeptide-3 solution, dipeptide diaminobutyryl benzylamide diacetate solution, and citric acid into vacuum homogenizing tank, stirring and dispersing;

Example 5

western pear flower extract solution: 10%, tea polyphenol: 5%, palmitoyl tripeptide-5 solution: 10%, arginine/lysine polypeptide solution: 10%, acetyl hexapeptide-8 solution: 10%, carnosine: 0.5%, oligopeptide-3 solution: 5 percent of dipeptide diaminobutyrylbenzylamide diacetate solution, 5 percent of phenoxyethanol solution: 5% and butanediol: 30%, citric acid: 0.5%, xanthan gum: 0.5% and the balance of ionized water.

step 1: adding deionized xanthan gum into vacuum homogenizing tank, homogenizing and stirring, heating to 80-85 deg.C, and maintaining for 30min;

and 2, step: cooling to 65 ℃, sequentially putting the butanediol and phenoxyethanol solution into a vacuum homogenizing tank, and stirring and dispersing uniformly;

and step 3: cooling to 45 deg.C, sequentially adding flos Pyri Calleryanae extract solution, tea polyphenols, palmitoyl tripeptide-5 solution, arginine/lysine polypeptide solution, acetyl hexapeptide-8 solution, carnosine, oligopeptide-3 solution, dipeptide diaminobutyryl benzylamide diacetate solution, and citric acid into vacuum homogenizing tank, stirring and dispersing;

Comparative example 1

Compared with the example 5, the palmitoyl tripeptide-5 solution in the raw materials is replaced by an arginine/lysine polypeptide solution, and the rest is the same as the example 5, and the raw materials comprise the following raw materials in percentage by mass:

western pear flower extract solution: 10%, tea polyphenols: 5%, arginine/lysine polypeptide solution: 20%, acetyl hexapeptide-8 solution: 10%, carnosine: 0.5%, oligopeptide-3 solution: 5 percent of dipeptide diaminobutyrylbenzylamide diacetate solution, 5 percent of phenoxyethanol solution: 5%, butanediol: 30%, citric acid: 0.5%, xanthan gum: 0.5% and the balance of ionized water.

Comparative example 2

Compared with the embodiment 5, the arginine/lysine polypeptide solution in the raw materials is replaced by acetyl hexapeptide-8 solution, and the rest is the same as the embodiment 5, and the raw materials comprise the following raw materials in percentage by mass:

western pear flower extract solution: 10%, tea polyphenol: 5%, palmitoyl tripeptide-5 solution: 10%, acetyl hexapeptide-8 solution: 20%, carnosine: 0.5%, oligopeptide-3 solution: 5 percent of dipeptide diaminobutyrylbenzylamide diacetate solution, 5 percent of phenoxyethanol solution: 5% and butanediol: 30%, citric acid: 0.5%, xanthan gum: 0.5% and the balance of ionized water.

Comparative example 3

Compared with the example 5, the acetyl hexapeptide-8 solution in the raw material is replaced by the oligopeptide-3 solution, and the rest is the same as the example 5, and the raw material comprises the following raw materials in percentage by mass:

american pear flower extract solution: 10%, tea polyphenols: 5%, palmitoyl tripeptide-5 solution: 10%, arginine/lysine polypeptide solution: 10%, carnosine: 0.5%, oligopeptide-3 solution: 15%, dipeptide diaminobutyrylbenzylamide diacetate solution: 5%, phenoxyethanol solution: 5% and butanediol: 30%, citric acid: 0.5%, xanthan gum: 0.5% and the balance of ionized water.

Comparative example 4

Compared with the embodiment 5, the oligopeptide-3 solution in the raw materials is replaced by a dipeptide diaminobutyrylbenzylamide diacetate solution, and the rest is the same as the embodiment 5, and the raw materials comprise the following components in percentage by mass:

american pear flower extract solution: 10%, tea polyphenol: 5%, palmitoyl tripeptide-5 solution: 10%, arginine/lysine polypeptide solution: 10%, acetyl hexapeptide-8 solution: 10%, carnosine: 0.5 percent, 10 percent of dipeptide diaminobutyrylbenzyl amide diacetate solution, phenoxyethanol solution: 5% and butanediol: 30%, citric acid: 0.5%, xanthan gum: 0.5% and the balance of ionized water.

Comparative example 5

Compared with the example 5, the dipeptide diaminobutyrylbenzylamide diacetate solution in the raw material is replaced by palmitoyl tripeptide-5 solution, and the rest is the same as the example 5, and the raw material comprises the following raw materials in percentage by mass:

american pear flower extract solution: 10%, tea polyphenol: 5%, palmitoyl tripeptide-5 solution: 15%, arginine/lysine polypeptide solution: 10%, acetyl hexapeptide-8 solution: 10%, carnosine: 0.5%, oligopeptide-3 solution: 5% phenoxyethanol solution: 5%, butanediol: 30%, citric acid: 0.5%, xanthan gum: 0.5% and the balance of ionized water.

The following tests were carried out for examples 1 to 5 and comparative examples 1 to 5:

test 1 comprehensive sensory evaluation method

In this test, sensory parameters of the obtained composition were evaluated by a sensory evaluation method (sensory evaluation indexes are shown in table 1), and sensory data obtained by a sensory evaluation method were evaluated by a sensory evaluation method. Fuzzy experiment volunteer selection criteria: selecting 60 persons, randomly dividing into 10 groups, and 6 persons in each group; the product is aged 22-35 years, is healthy, can accurately give use feeling after training, and cannot use other cosmetics 12 hours before testing.

TABLE 1 sensory evaluation index

Weight of each factor = { glossiness 0.1, selectivity 0.1, greasiness 0.1, spreadability 0.1, heaviness 0.1, absorbency 0.1, brightness 0.1, moisture retention 0.1, stickiness 0.1, residue 0.1}

And (3) product evaluation grade: a =80-100, b =70-80, c =50-60, d =0-50

The scores obtained from each test sample were averaged and the overall evaluation results are shown in table 2.

Table 2 comprehensive evaluation results are shown in

Experiment 2 double-blind random experiment

In the test, a double-blind random experiment method is adopted for evaluating the human body efficacy, and a multifunctional skin tester is used for measuring the skin condition parameters (including skin elasticity and skin moisture content) of a volunteer before use (day 0) and after use (weeks 1,2 and 3).

The using method comprises the following steps: the product is applied to face, gently applied to the face until absorption, and used once in the morning and evening for 3 weeks, and efficacy evaluation test is performed every week in the morning. The skin elasticity test and skin moisture test methods in the double-blind random experiment are as follows:

(1) Skin moisture content testing

The MPA5 software is opened, the Corneometer 10030770 button on the upper right corner is clicked, the probe cover is removed, the probe is vertically contacted with the skin which is cleaned or smeared with a sample, the test is started, the test result and the average value are displayed in a window after 1s, three different points are selected for testing and the average value of the water content change is shown in the table 3.

(2) Skin elasticity test

The skin elasticity probe of the multifunctional skin tester invented by German CK company is adopted for testing, firstly, the software of a Curometer Q is opened, a tool bar Measurement is clicked, after the pump is started, the probe cover is taken down, OK is clicked, the probe is vertically contacted with the skin which is cleaned and smeared with a sample, the test is started, the probe can be removed after the test cycle time is ended, the stored data can be exported by using an Excel table, and the average value of the skin elasticity change is shown in a table 3.

TABLE 3 mean values of moisture content and skin elasticity variation

As can be seen from table 2, the antioxidant and anti-aging composition containing the extract of the western pear flower, the tea polyphenol and the peptides provided by the invention has good comprehensive evaluation results, and on the basis of the extract of the western pear flower and the tea polyphenol, palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, oligopeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate are compounded, so that a subject has better use feeling under the synergistic action;

as can be seen from Table 3, the skin moisture content of the subjects in each test group was increased, indicating that the compound ingredient provided by the present invention can significantly increase the skin moisture content; from the average value of skin elasticity changes in table 3, it can be seen that an experimental group of the composition compounded with palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, oligopeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate is significantly higher than that of a comparative sample group on the basis of the western pear flower extract and tea polyphenol at the 7 th day of using the product, which shows that the synergistic effect of the western pear flower extract, the tea polyphenol compounded with palmitoyl tripeptide-5, arginine/lysine polypeptide, acetyl hexapeptide-8, oligopeptide-3 and dipeptide diaminobutyrylbenzylamide diacetate can improve the skin elasticity to a certain extent, so that a certain anti-aging effect is achieved.

In the description herein, references to the description of "one embodiment," "an example," "a specific example" or the like are intended to mean that a particular feature, structure, material, or characteristic described in connection with the embodiment or example is included in at least one embodiment or example of the invention. In this specification, the schematic representations of the terms used above do not necessarily refer to the same embodiment or example. Furthermore, the particular features, structures, materials, or characteristics described may be combined in any suitable manner in any one or more embodiments or examples.

The foregoing is illustrative and explanatory only, and it will be appreciated by those skilled in the art that various modifications, additions and substitutions can be made to the embodiments described without departing from the scope of the invention as defined in the appended claims.

Claims

1. An antioxidant and anti-aging composition containing a common pear flower extract, tea polyphenol and peptides is characterized by comprising the following raw materials in percentage by mass:

american pear flower extract solution: 1-20%, tea polyphenol: 1-5%, palmitoyl tripeptide-5 solution: 1-10%, arginine/lysine polypeptide solution: 1-10%, acetyl hexapeptide-8 solution: 1-10%, carnosine: 0.01-0.5%, oligopeptide-3 solution: 0.1-5%, dipeptide diaminobutyrylbenzylamide diacetate solution: 0.1-5%, phenoxyethanol solution: 0.5-5%, butanediol: 1-30%, citric acid: 0.01-0.5%, xanthan gum: 0.01-0.5% and the balance of ionized water.

2. The composition for resisting oxidation and aging containing the extract of the American pear flower, the tea polyphenol and the peptide as claimed in claim 1, wherein the mass percent of the solution of the extract of the American pear flower is 1-10%; the solution of the American pear flower extract comprises 69.4% of water, 20% of butanediol, 10% of the American pear flower extract and 0.6% of phenoxyethanol.

3. The composition for resisting oxidation and aging containing the extract of the American pear flower, the tea polyphenol and the peptide as claimed in claim 2, wherein the extraction method of the extract of the American pear flower comprises the following operations:

chopping dry American pear flowers, selecting ethanol with the mass percent of 70% as an extraction solvent, soaking the American pear flowers in the extraction solvent for 0.5h, then carrying out reflux extraction, wherein the reflux extraction temperature is 70-80 ℃, the extraction frequency is 2 times, the extraction time is 2 hours each time, and the material-liquid ratio of the first reflux extraction is 1:15, the material-liquid ratio of the second reflux extraction is 1:10; filtering the extracts, and mixing the filtrates; and concentrating the filtrate under reduced pressure, and vacuum drying the concentrated solution to obtain the extract of the American pear flower.

4. The composition of claim 1, wherein the arginine/lysine polypeptide solution comprises 0.0024% arginine/lysine polypeptide and 99.9976% deionized water.

5. The composition of claim 1, wherein the palmitoyl tripeptide-5 solution comprises palmitoyl tripeptide-50.5%, glycerol 62% and deionized water 37.5%.

6. The composition for resisting oxidation and aging containing the extract of the American pear flower, the tea polyphenol and the peptides as claimed in claim 1, wherein the acetyl hexapeptide-8 solution comprises 80.1% of acetyl hexapeptide, 96.87% of deionized water, 3% of 1, 2-hexanediol and 0.03% of ethylhexyl glycerin.

7. The composition of claim 1, wherein the dipeptide diaminobutyrobenzylamide diacetate solution comprises dipeptide diaminobutyrobenzylamide diacetate 0.1%, deionized water 96.87%, 1, 2-hexanediol 3%, and ethylhexylglycerin 0.03%.

8. The composition for resisting oxidation and aging containing the American pear flower extract, the tea polyphenol and the peptides as claimed in claim 1, wherein the oligopeptide-3 solution comprises oligopeptide-3.1%, 1, 2-pentanediol 5%, butanediol 11% and deionized water 83.9%.

9. The composition for resisting oxidation and aging containing the extract of the American pear flower, the tea polyphenol and the peptide as claimed in claim 1, wherein the phenoxyethanol solution comprises phenoxyethanol 90% and ethylhexylglycerin 10%.

10. A process for the preparation of an antioxidant anti-ageing composition comprising an extract of the flowers of avocado, tea polyphenols and peptides according to any one of claims 1 to 9, characterized in that it comprises the following steps:

step 1: adding deionized water and xanthan gum into vacuum homogenizing tank, homogenizing and stirring, heating to 80-85 deg.C, and maintaining for 30min;

step 2: cooling to 60-65 deg.C, sequentially adding butanediol and phenoxyethanol solution into vacuum homogenizing tank, stirring and dispersing;

and 3, step 3: cooling to 40-45 deg.C, sequentially adding flos Pyrariae Calvatae extract solution, tea polyphenols, palmitoyl tripeptide-5 solution, arginine/lysine polypeptide solution, acetyl hexapeptide-8 solution, carnosine, oligopeptide-3 solution, dipeptide diaminobutyryl benzyl amide diacetate solution, and citric acid into vacuum homogenizing tank, stirring, and dispersing;

and 4, step 4: after being stirred evenly, discharging after the pH value is tested to be qualified.