CN114836488B - Anti-wrinkle repair composition containing polypeptide and preparation method thereof - Google Patents
Anti-wrinkle repair composition containing polypeptide and preparation method thereof Download PDFInfo
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Abstract
The invention discloses a polypeptide-containing anti-wrinkle repair composition and a preparation method thereof, belongs to the technical field of cosmetic peptides, and particularly relates to a polypeptide-containing anti-wrinkle repair composition which is prepared by adopting a gluconic acid and vitamin C to prepare a cellulose C derivative under the action of a catalytic enzyme, then taking the cellulose C derivative as a group of components in an auxiliary agent, and then adding a functional polypeptide, a humectant, a pH regulator, an antibacterial stabilizer, a preservative and other auxiliary agents into water. The anti-wrinkle repair composition containing the polypeptide has good moisturizing performance, and has good protection effect after being applied to skin.
Description
Technical Field
The invention belongs to the technical field of beauty peptide, and particularly relates to an anti-wrinkle repair composition containing polypeptide and a preparation method thereof.
Background
The skin is an important and largest organ covering the whole body surface, is a protector of internal organs and tissues of the human body, is also an effect organ of the internal organs, spirit and surrounding environment, and plays an especially important role in the health and beauty of the human body.
Facial wrinkle enhancement is an important marker of aging. The formation of wrinkles is not simply due to the lapse of time. Of which aging is the leading cause of wrinkles. With age, sebaceous and sweat glands become less functional, the skin loses radiance, becomes dry, the subcutaneous fat also decreases, the skin begins to relax and become inelastic, so called "aging". However, the molecular mechanisms of facial skin aging are due to changes in the conformation of the triple helix of collagen, breakdown of elastin polypeptides, and disorganization of the lipid arrangement filling the skin, whatever the cause. The invention aims to provide an anti-wrinkle repair composition containing polypeptide, which can repair skin.
Disclosure of Invention
The invention aims to provide an anti-wrinkle repair composition containing polypeptide and having good moisturizing performance and good protection effect and a preparation method thereof.
The technical scheme adopted by the invention for realizing the purpose is as follows:
a method for preparing a cellulose C derivative, comprising: preparing a vitamin C derivative from the fructonic acid and the vitamin C under the action of catalytic enzyme; the use amount of the fructonic acid is 80-120wt% of the vitamin C.
Preferably, the catalytic enzyme is Lipozyme 435 lipase.
Preferably, the solvent in the preparation of the vitamin C derivative is t-butanol.
Preferably, in the preparation of the vitamin C derivative, the fructonic acid and the vitamin C are added into tert-butyl alcohol, then catalytic enzyme is added, the mixture is stirred and reacted for 16 to 48 hours at the temperature of 35 to 55 ℃, after the reaction is finished, the product is obtained by filtering, the tert-butyl alcohol is removed by rotary evaporation, the product is washed by saturated salt water and dried, and the vitamin C derivative is obtained.
More preferably, in the preparation of the vitamin C derivative, the amount of the cellulose C used is 10 to 20wt% of t-butanol.
More preferably, the amount of the fructonic acid used in the preparation of the vitamin C derivative is 80-120wt% of the vitamin C.
More preferably, in the preparation of the vitamin C derivative, the catalytic enzyme is Lipozyme 435 lipase, and the amount of the catalytic enzyme is 2-6wt% of the vitamin C.
The invention discloses a cellulose C derivative prepared by the method.
The invention discloses an anti-wrinkle repair composition containing polypeptide, which comprises the following components: contains the above-mentioned cellulose C derivative.
Preferably, the anti-wrinkle repair composition contains functional polypeptide, and the functional polypeptide is at least one of anti-expression wrinkle polypeptide, wrinkle-removing polypeptide, cell repair polypeptide and barrier repair polypeptide.
Preferably, the anti-wrinkle repair composition contains a humectant including at least one of hyaluronic acid, aloe vera gel, and glycerin.
Preferably, the anti-wrinkle repair composition contains a pH regulator, wherein the pH regulator is at least one of triethanolamine, sodium bicarbonate, potassium bicarbonate, arginine and citric acid.
Preferably, the anti-wrinkle repair composition contains an antibacterial stabilizer, and the antibacterial stabilizer is 1, 2-hexanediol.
Preferably, in the preparation of the anti-wrinkle modification composition containing the polypeptide, the functional polypeptide, the humectant, the pH regulator, the antibacterial stabilizer, the preservative and the auxiliary are added into water to be compounded to obtain the anti-wrinkle modification composition containing the polypeptide.
More preferably, the anti-wrinkle modification composition containing the polypeptide is prepared such that the functional polypeptide is used in an amount of 0.001-2wt% based on water.
More preferably, the humectant is used in an amount of 0.1-0.8wt% based on water in the preparation of the anti-wrinkle modifying composition containing the polypeptide.
More preferably, the pH adjusting agent adjusts the pH of the polypeptide-containing anti-wrinkle modification composition to 3-7 in the preparation of the polypeptide-containing anti-wrinkle modification composition.
More preferably, the anti-wrinkle modification composition containing the polypeptide is prepared such that the antibacterial stabilizer is used in an amount of 0.1 to 0.6wt% based on water.
More preferably, the anti-wrinkle modification composition containing the polypeptide is prepared such that the preservative is used in an amount of 0.03 to 0.12wt% based on water.
More preferably, the adjuvant is used in an amount of 0.05-0.3wt% of water in the preparation of the anti-wrinkle modification composition containing the polypeptide.
More preferably, in the preparation of the anti-wrinkle modifying composition containing the polypeptide, the functional polypeptide is at least one of an anti-expression wrinkle polypeptide, an anti-wrinkle polypeptide, a cell repair polypeptide and a barrier repair polypeptide. The expression resisting polypeptide, wrinkle removing polypeptide, cell repairing polypeptide and barrier repairing polypeptide in the functional polypeptide are expressed in a weight ratio of 0-1:0-1:0-1:0-1, and the use of the anti-expression line polypeptide, the wrinkle-removing polypeptide, the cell repairing polypeptide and the barrier repairing polypeptide is not 0 at the same time.
Still more preferably, the anti-wrinkle modification composition containing the polypeptide is prepared by adding at least one of acetyl hexapeptide-8, snake venom like peptide, acetyl hexapeptide-1, scorpion toxin and conotoxin to the anti-wrinkle type polypeptide. The expression resisting polypeptide contains acetyl hexapeptide-8, snake-like poison peptide, acetyl hexapeptide-1, red scorpion toxin and conotoxin in a ratio of 0-1:0-1:0-1:0-1:0-1, and the use ratio of acetyl hexapeptide-8, snake venom like peptide, acetyl hexapeptide-1, red scorpion toxin and conotoxin is not 0 at the same time. Acetyl hexapeptide-8, also known as ayurrin, a botulinum-like hormone, is an oligopeptide which imitates 6 amino acids at the N-terminal of SNAP-25 protein, can locally block the nerve transmission muscle contraction information, influences the skin sac nerve conduction, relaxes the facial muscles, and smoothes dynamic lines, static lines and fine lines, and particularly has an effect on relieving the periphery of eyes and fine wrinkles, and is known as skin external botulinum toxin without subcutaneous injection; snake venom-like peptides, also called snake venom tripeptides, act on the postsynaptic membrane and are reversible antagonists of the muscle nicotinic acetylcholine receptor (nmAChR). It binds to the epsilon subunit of nmAChR to block the binding of neurotransmitter acetylcholine to receptors, so that the excitement transmission is weakened, the muscles are relaxed due to the reduction of stimulation, and the anti-wrinkle effect is very excellent.
Still more preferably, in the preparation of the anti-wrinkle modifying composition containing the polypeptide, the wrinkle-removing polypeptide is at least one of palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2. The wrinkle-removing polypeptide contains palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, and acetyl tetrapeptide-2 in a proportion of 0-1:0-1:0-1:0-1:0-1:0-1, and the use of palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7, and acetyl tetrapeptide-2 is not 0 at the same time.
Palmitoyl tripeptide-1 is one of the cosmetic peptides which are applied earliest and used most widely, is a signal peptide, can promote the synthesis of matrix albumin (matrionic protein), especially collagen, and can increase the generation of elastin, hyaluronic acid, glycosaminoglycan and fibronectin; palmitoyl tripeptide-5 can promote skin cell growth, inhibit oxygen radicals and hydroxyl radicals from promoting matrix protein (matrionic acid protein), especially stimulate skin collagen synthesis through tissue growth factor (TGF-beta), and simultaneously protect collagen from being decomposed by matrix metalloproteinase, and the two synergistically act. Can also increase the production of elastin, hyaluronic acid, glycosaminoglycan and fibronectin; the collagen peptide promotes collagen synthesis by increasing stromal cell activity, making the skin appear more elastic and youthful. The function mode is tretinoin-like, but HAs no side effect of tretinoin, the synthesis of collagen and Hyaluronic Acid (HA) of intercellular substance is increased, fine lines are not seen, the skin is firm, the anti-wrinkle aims of smoothing wrinkles and firming the skin are achieved, and the skin quality is improved; palmitoyl pentapeptide-4 is known as palmitoyl pentapeptide-3, and is a polypeptide peptide which is the earliest and most widely applied in peptide series, namely the well-known pentapeptide or beautiful peptide. Palmitoyl pentapeptide-4 is a C-terminal fragment of collagen I in fact, has the efficacy similar to laser surgery, is the earliest messenger peptide applied, can stimulate the synthesis and development of collagen (I type, II type and IV type) and elastin in the dermis layer of the skin, and has very good nutritional and anti-wrinkle effects. Palmitoyl tetrapeptide-7 can delay and inhibit the production of excessive interleukins, thereby inhibiting some unnecessary inappropriate inflammatory responses and glycosylation damage. In vitro experiments, scientists found that palmitoyl tetrapeptide-7 induction exhibited a significant dose-dependent decrease in interleukin production. The higher the dose of palmitoyl tetrapeptide-7, the more the reduction of interleukin, the higher the reduction of interleukin by up to 40%. The production of interleukins was promoted by UV radiation, and 86% reduction in interleukins was seen when the cells were exposed to UV radiation and then treated with palmitoyl tetrapeptide-7. Palmitoyl tetrapeptide-7 can promote the growth of connective tissues and increase the production of collagen in the skin; when collagen is increased, the skin can heal and regenerate itself.
Still more preferably, in the preparation of the anti-wrinkle modifying composition containing the polypeptide, the cell repairing polypeptide is at least one of trifluoroacetyl tripeptide-2, carnosine and decarboxylated carnosine. The trifluoroacetyl tripeptide-2, carnosine and decarboxylated carnosine in the cell repair polypeptide are expressed in a weight ratio of 0-1:0-1:0-1, and the trifluoroacetyl tripeptide-2, carnosine and decarboxylated carnosine are not simultaneously 0. Nonapeptide-1 is a biomimetic peptide, which has a good match with the MC1 receptor on melanocytes, and thus can be used as an antagonist of melanocyte stimulating hormone to competitively bind with the MC1 receptor and prevent tyrosinase from being further activated to produce melanin. Nonapeptide-1 competitively seals the entrance of the receptor and various factor signals on the melanoblast, weakens the activity of the melanoblast, reduces the melanin production amount, and plays the effects of homogenizing and brightening the skin color; carnosine is a naturally occurring dipeptide that is found in tissues such as skeletal muscle and brain of vertebrates and consists of beta-alanine and histidine. It can take part in peroxidation in cells and scavenge free radicals, and has stronger antioxidant capacity than Vc and VE. The carnosine has the effects of preventing skin aging, brightening skin color, recovering skin elasticity, repairing and filling fine wrinkles, and particularly has obvious yellow removing effect on mature skin; the tetrapeptide-30, also called as skin lightening peptide, is an oligopeptide consisting of four amino acids, has a rapid and effective mechanism for lightening skin color, can lighten skin by reducing the number of tyrosinase and inhibiting the activation of melanocytes, and is suitable for skin with various skin colors. The skin can reduce melanin deposition by inhibiting MSH (melanocyte stimulating hormone), reducing melanin generation, and preventing excessive melanin production.
Still more preferably, in the preparation of the anti-wrinkle modification composition containing the polypeptide, the barrier repair polypeptide is at least one of hexapeptide-9, acetyl heptapeptide-4 and oligopeptide-1. In the barrier repair polypeptide, the weight ratio of hexapeptide-9, acetyl heptapeptide-4 and oligopeptide-1 is 0-1:0-1:0-1, and the hexapeptide-9, acetyl heptapeptide-4 and oligopeptide-1 are not simultaneously 0.
Still more preferably, in the preparation of the anti-wrinkle modifying composition containing the polypeptide, the humectant includes at least one of hyaluronic acid, aloe vera gel, and glycerin. The humectant contains hyaluronic acid, aloe gel and glycerin in a proportion of 0-1:0-1:0-1, and hyaluronic acid, aloe gel, and glycerol are not 0 at the same time.
Still more preferably, in the preparation of the anti-wrinkle modification composition containing polypeptide, the pH regulator is at least one of triethanolamine, sodium bicarbonate, potassium bicarbonate, arginine, and citric acid. The pH regulator comprises triethanolamine, sodium bicarbonate, potassium bicarbonate, arginine and citric acid in a proportion of 0-1:0-1:0-1:0-1:0-1, and the use of triethanolamine, sodium bicarbonate, potassium bicarbonate, arginine, and citric acid is not 0 at the same time.
Still more preferably, in the preparation of the anti-wrinkle modification composition containing the polypeptide, 1, 2-hexanediol is selected as the antibacterial stabilizer.
Still more preferably, in the preparation of the anti-wrinkle modifying composition containing the polypeptide, the preservative is ethylhexyl glycerol.
Still more preferably, the anti-wrinkle modification composition containing the polypeptide is prepared by using an adjuvant containing at least vitamin C derivative and Tween-20, and containing at least one or none of butanediol, caprylyl glycol, butyryl hydroxypentanoic acid, phenoxyethanol, arbutin and tyrosine kinase inhibitor. In the auxiliary agent, the vitamin C derivative and Tween-20 are mixed according to the mass ratio of 0-1:0-1, and the use amount of the vitamin C derivative and the Tween-20 is not 0; the adjuvants include butanediol, caprylyl glycol, butyryl hydroxyvaleric acid, phenoxyethanol, arbutin, and tyrosine kinase inhibitor in a ratio of 0-1:0-1:0-1:0-1:0-1:0-1, wherein any one of butanediol, caprylyl glycol, butyryl hydroxyvaleric acid, phenoxyethanol, arbutin and tyrosine kinase inhibitor is 0-100wt% of vitamin C derivative. The vitamin C derivative is generated by the action of the catalytic enzyme of the fructonic acid and the vitamin C, the vitamin C derivative and the Tween-20 are used as fixing auxiliary agents and are prepared with other components to form the anti-wrinkle repairing composition, the moisturizing performance is improved, and the anti-wrinkle repairing composition has a better skin protection effect after being applied to the skin for treatment.
The invention discloses application of the cellulose C derivative in preparing cosmetics.
The invention adopts the reaction of the fructonic acid and the cellulose C to prepare the cellulose C derivative, then the cellulose C derivative is used as a group of components in the auxiliary agent, and then the cellulose C derivative is mixed with the functional polypeptide, the humectant, the pH regulator, the antibacterial stabilizer, the preservative and other auxiliary agents in water to prepare the anti-wrinkle repair composition containing the polypeptide, thereby having the following beneficial effects: the anti-wrinkle repair composition containing the polypeptide has good moisturizing performance, and has good protection effect after being applied to skin. Therefore, the anti-wrinkle repair composition containing the polypeptide and the preparation method thereof have good moisturizing performance and good protection effect.
Drawings
FIG. 1 is an infrared spectrum of a cellulose C derivative;
FIG. 2 is a graph of the moisturizing performance of an anti-wrinkle repair composition comprising a polypeptide;
FIG. 3 is a graph of TEWL values of skin treated with an anti-wrinkle repair composition comprising a polypeptide.
Detailed Description
The technical solution of the present invention is further described in detail below with reference to the following detailed description and the accompanying drawings:
example 1:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24h at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt water, and drying to obtain the vitamin C derivative. The usage amount of the cellulose C is 16wt% of the tertiary butanol, the usage amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the usage amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is an anti-expression print polypeptide. The anti-expression grain polypeptide is acetyl hexapeptide-8. The humectant comprises hyaluronic acid and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative and Tween-20, and also contains butanediol, octaethylene glycol, and butyryl hydroxypentanoic acid. The usage amount of the functional polypeptide is 0.12wt% of water, the usage amount of the humectant is 0.4wt% of water, and the ratio of hyaluronic acid and glycerol in the humectant is 1:0.8, the pH regulator is used for regulating the pH value of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the auxiliary agent is used in an amount of 0.15wt% of water, and in the auxiliary agent, the ratio of butanediol, caprylyl glycol and butyryl hydroxypentanoic acid is 1:0.3: a mass ratio of 0.8 was used. In the adjuvant, the vitamin C derivative and Tween-20 are mixed in a ratio of 1:0.3, the use ratio of the vitamin C derivative to the butanediol in the auxiliary agent is 1:0.6.
example 2:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24 hours at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt solution, and drying to obtain the vitamin C derivative. The using amount of the cellulose C is 16wt% of the tertiary butanol, the using amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the using amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition. The functional polypeptide is wrinkle-removing polypeptide. The wrinkle-removing polypeptide is palmitoyl pentapeptide-4. The humectant comprises hyaluronic acid and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is ethyl hexyl glycerol. The adjuvant at least contains vitamin C derivative and Tween-20, and also contains butanediol, octaethylene glycol, and butyryl hydroxypentanoic acid. The usage amount of the functional polypeptide is 0.12wt% of water, the usage amount of the humectant is 0.4wt% of water, and the ratio of hyaluronic acid and glycerol in the humectant is 1:0.8, a pH regulator for regulating the pH of the polypeptide-containing anti-wrinkle modification composition to 5, an antibacterial stabilizer in an amount of 0.3wt% of water, a preservative in an amount of 0.09wt% of water, and an adjuvant in an amount of 0.15wt% of water, wherein in the adjuvants, butanediol, octylglycol, butyrylhydroxyvaleric acid are mixed in a mass ratio of 1:0.3: a mass ratio of 0.8 was used. In the adjuvant, the vitamin C derivative and Tween-20 are mixed in a ratio of 1:0.3, the use ratio of the vitamin C derivative to the butanediol in the auxiliary agent is 1:0.6.
example 3:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24 hours at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt solution, and drying to obtain the vitamin C derivative. The usage amount of the cellulose C is 16wt% of the tertiary butanol, the usage amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the usage amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is a cell repair polypeptide. The cell repair polypeptide is trifluoroacetyl tripeptide-2. The humectant comprises hyaluronic acid and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative and Tween-20, and contains butanediol, octaethylene glycol, and butyryl hydroxypentanoic acid. The usage amount of the functional polypeptide is 0.12wt% of water, the usage amount of the humectant is 0.4wt% of water, and the ratio of hyaluronic acid and glycerol in the humectant is 1:0.8, the pH regulator is used for regulating the pH value of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the auxiliary agent is used in an amount of 0.15wt% of water, and in the auxiliary agent, the ratio of butanediol, caprylyl glycol and butyryl hydroxypentanoic acid is 1:0.3: a mass ratio of 0.8 was used. Vitamin C derivative and tween-20 in the adjuvant are mixed in a ratio of 1:0.3, the use ratio of the vitamin C derivative to the butanediol in the auxiliary agent is 1:0.6.
example 4:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24h at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt water, and drying to obtain the vitamin C derivative. The using amount of the cellulose C is 16wt% of the tertiary butanol, the using amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the using amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is a barrier repair polypeptide. The barrier repair polypeptide is hexapeptide-9. The humectant comprises hyaluronic acid and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative and Tween-20, and also contains butanediol, octaethylene glycol, and butyryl hydroxypentanoic acid. The usage amount of the functional polypeptide is 0.12wt% of water, the usage amount of the humectant is 0.4wt% of water, and the ratio of hyaluronic acid and glycerol in the humectant is 1:0.8, the pH regulator is used for regulating the pH value of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the auxiliary agent is used in an amount of 0.15wt% of water, and in the auxiliary agent, the ratio of butanediol, caprylyl glycol and butyryl hydroxypentanoic acid is 1:0.3: a mass ratio of 0.8 was used. Vitamin C derivative and tween-20 in the adjuvant are mixed in a ratio of 1:0.3, the use ratio of the vitamin C derivative to the butanediol in the auxiliary agent is 1:0.6.
example 5:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24h at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt water, and drying to obtain the vitamin C derivative. The usage amount of the cellulose C is 16wt% of the tertiary butanol, the usage amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the usage amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition. The functional polypeptide is anti-expression wrinkle polypeptide and wrinkle-removing polypeptide. The anti-expression grain polypeptide is snake-like poison peptide. The wrinkle-removing polypeptide is palmitoyl hexapeptide-12. The humectant comprises hyaluronic acid and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is ethyl hexyl glycerol. The adjuvant at least contains vitamin C derivative and Tween-20, and contains butanediol, octaethylene glycol, and butyryl hydroxypentanoic acid. The usage amount of the functional polypeptide is 0.24wt% of water, and the anti-expression wrinkle polypeptide and the wrinkle-removing polypeptide in the functional polypeptide are represented by the following ratio of 1:1, the usage amount of the humectant is 0.4wt% of water, and the weight ratio of hyaluronic acid and glycerol in the humectant is 1:0.8, the pH regulator is used for regulating the pH value of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the auxiliary agent is used in an amount of 0.15wt% of water, and in the auxiliary agent, the ratio of butanediol, caprylyl glycol and butyryl hydroxypentanoic acid is 1:0.3: a mass ratio of 0.8 was used. Vitamin C derivative and tween-20 in the adjuvant are mixed in a ratio of 1:0.3, and the use ratio of the vitamin C derivative to the butanediol in the auxiliary agent is 1:0.6.
example 6:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24h at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt water, and drying to obtain the vitamin C derivative. The usage amount of the cellulose C is 16wt% of the tertiary butanol, the usage amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the usage amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is anti-expression wrinkle polypeptide, wrinkle-removing polypeptide, and cell repairing polypeptide. The anti-expression grain polypeptide is acetyl hexapeptide-1. The wrinkle-removing polypeptide is palmitoyl tripeptide-5. The cell repair polypeptide is trifluoroacetyl tripeptide-2. The humectant comprises hyaluronic acid and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is ethyl hexyl glycerol. The adjuvant at least contains vitamin C derivative and Tween-20, and also contains butanediol, octaethylene glycol, and butyryl hydroxypentanoic acid. The usage amount of the functional polypeptide is 0.36wt% of water, and the expression-resisting polypeptide, wrinkle-removing polypeptide and cell repairing polypeptide in the functional polypeptide are represented by the following ratio of 1:0.8:1.2, the amount of the humectant is 0.4wt% of water, and the weight ratio of hyaluronic acid and glycerol in the humectant is 1:0.8, a pH regulator for regulating the pH of the polypeptide-containing anti-wrinkle modification composition to 5, an antibacterial stabilizer in an amount of 0.3wt% of water, a preservative in an amount of 0.09wt% of water, and an adjuvant in an amount of 0.15wt% of water, wherein in the adjuvants, butanediol, octylglycol, butyrylhydroxyvaleric acid are mixed in a mass ratio of 1:0.3: a mass ratio of 0.8 was used. In the adjuvant, the vitamin C derivative and Tween-20 are mixed in a ratio of 1:0.3, the use ratio of the vitamin C derivative to the butanediol in the auxiliary agent is 1:0.6.
example 7:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24 hours at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt solution, and drying to obtain the vitamin C derivative. The usage amount of the cellulose C is 16wt% of the tertiary butanol, the usage amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the usage amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is anti-expression wrinkle polypeptide, wrinkle-removing polypeptide, cell repairing polypeptide, and barrier repairing polypeptide. The expression pattern resisting polypeptide is conotoxin. The wrinkle-removing polypeptide is acetyl tetrapeptide-2. The cell repair polypeptide is decarboxylated carnosine. The barrier repair polypeptide is oligopeptide-1. The humectant comprises hyaluronic acid, aloe gel, and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative and Tween-20, and contains butyryl hydroxyvaleric acid, phenoxyethanol, arbutin, and tyrosine kinase inhibitor. The usage amount of the functional polypeptide is 0.36wt% of water, and the expression resisting polypeptide, wrinkle removing polypeptide, cell repairing polypeptide and barrier repairing polypeptide in the functional polypeptide are represented by the formula (1: 0.6:0.6:1.2, the using amount of the humectant is 0.5wt% of water, and the weight ratio of hyaluronic acid, aloe gel and glycerol in the humectant is 1:0.6:0.4, a pH regulator for regulating the pH of the anti-wrinkle modification composition containing the polypeptide to 5, an antibacterial stabilizer in an amount of 0.3wt% of water, a preservative in an amount of 0.09wt% of water, and an adjuvant in an amount of 0.21wt% of water, wherein the vitamin C derivative and Tween-20 in the adjuvant are in a weight ratio of 1:0.3, and the auxiliary agents comprise butyryl hydroxy valeric acid, phenoxyethanol, arbutin and a tyrosine kinase inhibitor in a mass ratio of 1:0.6:0.9:1.5, the use ratio of the vitamin C derivative to the butyrylhydroxyvaleric acid in the auxiliary agent is 1:0.6.
example 8:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24 hours at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt solution, and drying to obtain the vitamin C derivative. The using amount of the cellulose C is 16wt% of the tertiary butanol, the using amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the using amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition containing polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is wrinkle-removing polypeptide, cell repairing polypeptide, and barrier repairing polypeptide. The wrinkle-removing polypeptide comprises palmitoyl pentapeptide-4, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2, wherein the proportion of the palmitoyl pentapeptide-4, the palmitoyl tetrapeptide-7 and the acetyl tetrapeptide-2 in the wrinkle-removing polypeptide is 1:1: a mass ratio of 1 was used. The cell repair polypeptide is trifluoroacetyl tripeptide-2, carnosine and decarboxylated carnosine, and the trifluoroacetyl tripeptide-2, the carnosine and the decarboxylated carnosine in the cell repair polypeptide are mixed according to a mass ratio of 1:0.6: a mass ratio of 1.2 was used. The barrier repair polypeptides are hexapeptide-9 and oligopeptide-1, wherein the ratio of hexapeptide-9 to oligopeptide-1 in the barrier repair polypeptides is 1: a mass ratio of 0.8 was used.
The humectant comprises hyaluronic acid, aloe gel, and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative and Tween-20, and contains butyryl hydroxyvaleric acid, phenoxyethanol, arbutin, and tyrosine kinase inhibitor. The use amount of the functional polypeptide is 0.52wt% of water, and the wrinkle-removing polypeptide, the cell repairing polypeptide and the barrier repairing polypeptide in the functional polypeptide are represented by the formula 1:0.6:1.2, the using amount of the humectant is 0.5wt% of water, and the weight ratio of hyaluronic acid, aloe gel and glycerol in the humectant is 1:0.6:0.4, the pH regulator regulates the pH of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the adjuvant is used in an amount of 0.21wt% of water, and the vitamin C derivative and the Tween-20 in the adjuvant are mixed in a weight ratio of 1:0.3, and the auxiliary agents comprise butyryl hydroxy valeric acid, phenoxyethanol, arbutin and a tyrosine kinase inhibitor in a mass ratio of 1:0.6:0.9:1.5, the use ratio of the vitamin C derivative to the butyrylhydroxyvaleric acid in the auxiliary agent is 1:0.6.
example 9:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24h at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt water, and drying to obtain the vitamin C derivative. The using amount of the cellulose C is 16wt% of the tertiary butanol, the using amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the using amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition. The functional polypeptide is anti-expression wrinkle polypeptide, wrinkle-removing polypeptide and barrier repair polypeptide. The anti-expression line polypeptide is acetyl hexapeptide-8, snake-like venom peptide, acetyl hexapeptide-1, red scorpion toxin and conotoxin, and the anti-expression line polypeptide is acetyl hexapeptide-8, snake-like venom peptide, acetyl hexapeptide-1, red scorpion toxin and conotoxin, wherein the ratio of acetyl hexapeptide-8 to the snake-like venom peptide to the acetyl hexapeptide to the conotoxin is 1:1:1:1:1 was used. The wrinkle-removing polypeptide is palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7, wherein the content of palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1 and palmitoyl tetrapeptide-7 in the wrinkle-removing polypeptide is 1:1:1:1: a mass ratio of 1 was used. The barrier repair polypeptide is hexapeptide-9, acetyl heptapeptide-4 and oligopeptide-1, wherein the hexapeptide-9, acetyl heptapeptide-4 and oligopeptide-1 in the barrier repair polypeptide are in a proportion of 1:1: a mass ratio of 1 was used.
The humectant comprises hyaluronic acid, aloe gel, and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative and Tween-20, and contains butyryl hydroxyvaleric acid, phenoxyethanol, arbutin, and tyrosine kinase inhibitor. The using amount of the functional polypeptide is 0.52wt% of water, and the anti-expression wrinkle polypeptide, the wrinkle-removing polypeptide and the barrier repair polypeptide in the functional polypeptide are expressed by the weight ratio of 1:0.6:1.2, the amount of the humectant is 0.5wt% of water, and the weight ratio of hyaluronic acid, aloe gel and glycerol in the humectant is 1:0.6:0.4, the pH regulator regulates the pH of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the adjuvant is used in an amount of 0.21wt% of water, and the vitamin C derivative and the Tween-20 in the adjuvant are mixed in a weight ratio of 1:0.3, and the auxiliary agents comprise butyryl hydroxyvaleric acid, phenoxyethanol, arbutin and a tyrosine kinase inhibitor in a mass ratio of 1:0.6:0.9:1.5, the use ratio of the vitamin C derivative to the butyrylhydroxyvaleric acid in the auxiliary agent is 1:0.6.
example 10:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24h at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt water, and drying to obtain the vitamin C derivative. The usage amount of the cellulose C is 16wt% of the tertiary butanol, the usage amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the usage amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition containing polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is anti-expression wrinkle polypeptide, wrinkle-removing polypeptide, cell repairing polypeptide, and barrier repairing polypeptide. The anti-expression line polypeptide is acetyl hexapeptide-8, snake-like poison peptide, acetyl hexapeptide-1, red scorpion toxin and conotoxin, and the anti-expression line polypeptide is characterized in that the ratio of acetyl hexapeptide-8, snake-like poison peptide, acetyl hexapeptide-1, red scorpion toxin and conotoxin is 1:1:1:1: a mass ratio of 1 was used. The wrinkle-removing polypeptide is palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2, wherein the wrinkle-removing polypeptide comprises palmitoyl pentapeptide-4, palmitoyl hexapeptide-12, palmitoyl tripeptide-5, palmitoyl tripeptide-1, palmitoyl tetrapeptide-7 and acetyl tetrapeptide-2 in a ratio of 1:1:1:1:1:1 was used. The cell repair polypeptide is trifluoroacetyl tripeptide-2, carnosine and decarboxylated carnosine, wherein the trifluoroacetyl tripeptide-2, the carnosine and the decarboxylated carnosine in the cell repair polypeptide are expressed by the ratio of 1:1: a mass ratio of 1 was used. The barrier repair polypeptide is hexapeptide-9, acetyl heptapeptide-4 and oligopeptide-1. The humectant comprises hyaluronic acid, aloe gel, and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is ethyl hexyl glycerol. The adjuvant at least contains vitamin C derivative and Tween-20, and contains butyryl hydroxyvaleric acid, phenoxyethanol, arbutin, and tyrosine kinase inhibitor. The using amount of the functional polypeptide is 0.52wt% of water, and the anti-expression wrinkle polypeptide, the wrinkle-removing polypeptide and the barrier repair polypeptide in the functional polypeptide are expressed by the weight ratio of 1:0.6:1.2, the using amount of the humectant is 0.5wt% of water, and the weight ratio of hyaluronic acid, aloe gel and glycerol in the humectant is 1:0.6:0.4, the pH regulator regulates the pH of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the adjuvant is used in an amount of 0.21wt% of water, and the vitamin C derivative and the Tween-20 in the adjuvant are mixed in a weight ratio of 1:0.3, and the auxiliary agents comprise butyryl hydroxyvaleric acid, phenoxyethanol, arbutin and a tyrosine kinase inhibitor in a mass ratio of 1:0.6:0.9:1.5, the use ratio of the vitamin C derivative to the butyrylhydroxyvaleric acid in the auxiliary agent is 1:0.6.
example 11:
a method for preparing an anti-wrinkle modifying composition containing polypeptide,
in this example, cetyl lactate was used as an adjuvant in the preparation of the anti-wrinkle modification composition containing a polypeptide, and the amount of cetyl lactate used was 0.02 to 0.08wt% of water. In the presence of vitamin C derivative, tween-20 and other components, cetyl lactate is further added to obtain the anti-wrinkle modifying composition with better moisturizing performance and better skin protecting effect.
Preparation of vitamin C derivatives: adding the fructonic acid and the vitamin C into tert-butyl alcohol, then adding catalytic enzyme, stirring and reacting for 24 hours at the temperature of 50 ℃, filtering after the reaction is finished, removing the tert-butyl alcohol by rotary evaporation to obtain a product, washing with saturated salt solution, and drying to obtain the vitamin C derivative. The using amount of the cellulose C is 16wt% of the tertiary butanol, the using amount of the fructonic acid is 80wt% of the vitamin C, the catalytic enzyme is Lipozyme 435 lipase, and the using amount of the catalytic enzyme is 4wt% of the vitamin C.
Preparation of anti-wrinkle modifying composition comprising polypeptide: adding functional polypeptide, humectant, pH regulator, antibacterial stabilizer, antiseptic and adjuvant into water, and compounding to obtain the composition containing polypeptide for improving wrinkle resistance. The functional polypeptide is anti-expression wrinkle polypeptide, wrinkle-removing polypeptide, cell repairing polypeptide, and barrier repairing polypeptide. The expression pattern resisting polypeptide is conotoxin. The wrinkle-removing polypeptide is acetyl tetrapeptide-2. The cell repair polypeptide is decarboxylated carnosine. The barrier repair polypeptide is oligopeptide-1. The humectant comprises hyaluronic acid, aloe gel, and glycerol. The pH regulator is arginine. 1, 2-hexanediol is selected as the antibacterial stabilizer. The preservative is selected from ethylhexyl glycerin. The adjuvant at least contains vitamin C derivative, cetyl lactate and tween-20, and contains butyryl hydroxyvaleric acid, phenoxyethanol, arbutin, and tyrosine kinase inhibitor. The using amount of the functional polypeptide is 0.36wt% of water, and the expression-resisting polypeptide, wrinkle-removing polypeptide, cell repairing polypeptide and barrier repairing polypeptide in the functional polypeptide are expressed in a proportion of 1:0.6:0.6:1.2, the amount of the humectant is 0.5wt% of water, and the weight ratio of hyaluronic acid, aloe gel and glycerol in the humectant is 1:0.6:0.4, the pH regulator regulates the pH value of the anti-wrinkle modification composition containing the polypeptide to 5, the antibacterial stabilizer is used in an amount of 0.3wt% of water, the preservative is used in an amount of 0.09wt% of water, the adjuvant is used in an amount of 0.24wt% of water, and the vitamin C derivative and the Tween-20 in the adjuvant are mixed in a weight ratio of 1:0.3, and the auxiliary agents comprise butyryl hydroxyvaleric acid, phenoxyethanol, arbutin and a tyrosine kinase inhibitor in a mass ratio of 1:0.6:0.9:1.5, the use ratio of the vitamin C derivative to the butyrylhydroxyvaleric acid in the auxiliary agent is 1:0.6, in addition, the total usage amount of vitamin C derivative and Tween-20, butyryl hydroxy valeric acid, phenoxyethanol, arbutin and tyrosine kinase inhibitor in the adjuvant is 0.21 percent of water, so that the usage amount of the cetyl lactate is 0.03 percent of the water by weight.
Comparative example 1:
this comparative example differs from example 7 only in that no vitamin C derivative is used in the adjuvant.
Test example:
1. infrared characterization
Test samples: the vitamin C derivative prepared in example 1.
The infrared characterization of the vitamin C derivative prepared by the invention is shown in figure 1, wherein the infrared characterization is 3382cm -1 Infrared absorption peak of hydroxyl at 2968cm -1 The infrared absorption peak of the methyl is 2859cm -1 Is a methylene infrared absorption peak at 1728cm -1 The infrared absorption peak of carbonyl indicates that the vitamin C derivative is obtained.
2. Test for moisture retention
Test samples: the anti-wrinkle modification compositions containing polypeptides prepared in the examples and comparative examples.
The test method comprises the following steps: uniformly coating a test sample on a glass plate, weighing, standing for 1h under the conditions of constant temperature, 25 ℃ and constant humidity of 50%, and weighing.
The water loss rate is calculated according to the following formula:
water loss rate = (weight before smear placement-weight after smear placement)/(weight before smear placement-glass plate weight) × 100%.
The results of the partial water loss of the polypeptide-containing anti-wrinkle modification composition prepared by the methods of the present invention are shown in fig. 2, where a is example 1, b is example 2, c is example 3, d is example 4, e is example 5, f is comparative example 1, the water loss of the polypeptide-containing anti-wrinkle modification composition prepared in example 7 is 81%, the water loss of the polypeptide-containing anti-wrinkle modification composition prepared in comparative example 1 is 89.73%, and example 7 shows that the polypeptide-containing anti-wrinkle modification composition prepared in example 7 has better moisture retention performance than comparative example 1, and the water loss of the polypeptide-containing anti-wrinkle modification composition prepared in examples 8-10 is lower than that of the polypeptide-containing anti-wrinkle modification composition prepared in example 7, which shows that the polypeptide-containing anti-wrinkle modification composition prepared by the methods of the present invention has better moisture retention performance; the water loss rate of the anti-wrinkle modification composition containing the polypeptide prepared in example 11 is 77.25%, and the comparison between example 11 and example 7 shows that the anti-wrinkle modification composition containing the polypeptide prepared in example 11 has better moisture retention by further using cetyl lactate as an additive on the basis of the technical scheme of example 7.
The water loss rate of the anti-wrinkle modification composition containing the polypeptide prepared by the invention is below 85%.
3. Measurement of epidermal Water loss
Test samples: the anti-wrinkle modification compositions containing polypeptides prepared in the examples and comparative examples.
The test method comprises the following steps: a test area is defined on the forearm skin, the TEWL value of blank skin in the blank area is tested, and then the test sample is smeared on the designated area and is tested 60min after use. The amount of the test sample used per area was 2mg/cm 2 。
A larger skin TEWL indicates a weaker barrier function of the skin and a poorer self-protective ability. The results of TEWL values of the polypeptide-containing anti-wrinkle modification composition prepared by the methods of the present invention after being applied to the skin are shown in FIG. 3, in which A is example 1, B is example 2, C is example 3, D is example 4, E is example 5, F is comparative example 1, TEWL value of the skin of the blank group is 9.23 g.h -1 m -2 The TEWL value of the skin treated with the anti-wrinkle modification composition containing the polypeptide prepared in example 7 was 7.42 g.h -1 m -2 Comparative example 1 the TEWL value of the skin treated with the anti-wrinkle modification composition containing the polypeptide prepared in comparative example 1 was 8.69 g-h -1 m -2 Example 7 shows that the anti-wrinkle modification composition containing the polypeptide prepared in example 7 has better skin protection performance compared with comparative example 1, and the TEWL values of the skin after the anti-wrinkle modification composition containing the polypeptide prepared in examples 8-10 are all lower than the TEWL values of the skin after the anti-wrinkle modification composition containing the polypeptide prepared in example 7, which shows that the anti-wrinkle modification composition containing the polypeptide prepared by the method of the invention has skin protection performance; example 11 the anti-wrinkle modification composition with the polypeptide prepared in example 11 has a TEWL value of 6.69 g.h -1 m -2 Example 11 compares with example 7, and shows that based on the technical scheme of example 7, further using cetyl lactate as an additive, the obtained anti-wrinkle modification composition containing polypeptide has better skin protection performance.
The TEWL value of the skin treated by the anti-wrinkle modification composition containing the polypeptide is 6.5-8.5 g.h -1 m -2 ，
The above embodiments are merely illustrative, and not restrictive, and those skilled in the art can make various changes and modifications without departing from the spirit and scope of the invention. Therefore, all equivalent technical solutions also belong to the scope of the present invention, and the protection scope of the present invention should be defined by the claims.
Claims (8)
1. A method for preparing a vitamin C derivative, comprising: preparing a vitamin C derivative from the fructonic acid and the vitamin C under the action of catalytic enzyme; the use amount of the fructonic acid is 80-120wt% of the vitamin C; the catalytic enzyme is Lipozyme 435 lipase, and a solvent in the preparation of the vitamin C derivative is tert-butyl alcohol.
2. The vitamin C derivative produced by the process of claim 1.
3. An anti-wrinkle repair composition comprising a polypeptide, comprising: comprising the vitamin C derivative according to claim 2.
4. The anti-wrinkle repair composition comprising a polypeptide according to claim 3, wherein: the anti-wrinkle repair composition contains functional polypeptide, and the functional polypeptide is at least one of anti-expression wrinkle polypeptide, wrinkle-removing polypeptide, cell repair polypeptide and barrier repair polypeptide.
5. The anti-wrinkle repair composition comprising a polypeptide according to claim 3, wherein: the anti-wrinkle repair composition contains a humectant which comprises at least one of hyaluronic acid, aloe gel and glycerol.
6. The anti-wrinkle repair composition comprising a polypeptide according to claim 3, wherein: the anti-wrinkle repair composition contains a pH regulator, wherein the pH regulator is at least one of triethanolamine, sodium bicarbonate, potassium bicarbonate, arginine and citric acid.
7. The anti-wrinkle repair composition comprising a polypeptide according to claim 3, wherein: the anti-wrinkle repair composition contains an antibacterial stabilizer, and the antibacterial stabilizer is 1, 2-hexanediol.
8. Use of the vitamin C derivative as claimed in claim 2 for the preparation of cosmetics.
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