CN115486448B - Low-temperature disinfection solution for inactivating SARS-CoV-2 virus at-80 DEG C - Google Patents
Low-temperature disinfection solution for inactivating SARS-CoV-2 virus at-80 DEG C Download PDFInfo
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- CN115486448B CN115486448B CN202211139434.0A CN202211139434A CN115486448B CN 115486448 B CN115486448 B CN 115486448B CN 202211139434 A CN202211139434 A CN 202211139434A CN 115486448 B CN115486448 B CN 115486448B
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- 238000004659 sterilization and disinfection Methods 0.000 title claims abstract description 26
- 230000000415 inactivating effect Effects 0.000 title abstract description 5
- 241001678559 COVID-19 virus Species 0.000 title 1
- 239000000645 desinfectant Substances 0.000 claims abstract description 105
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims abstract description 84
- KFZMGEQAYNKOFK-UHFFFAOYSA-N Isopropanol Chemical compound CC(C)O KFZMGEQAYNKOFK-UHFFFAOYSA-N 0.000 claims abstract description 70
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 27
- 239000007788 liquid Substances 0.000 claims abstract description 11
- 230000001954 sterilising effect Effects 0.000 claims description 31
- 238000000034 method Methods 0.000 claims description 10
- 208000001528 Coronaviridae Infections Diseases 0.000 claims description 2
- 230000002708 enhancing effect Effects 0.000 claims description 2
- 238000005507 spraying Methods 0.000 claims 1
- 241000700605 Viruses Species 0.000 abstract description 13
- 241000711573 Coronaviridae Species 0.000 abstract description 11
- 235000013305 food Nutrition 0.000 abstract description 5
- 241000894006 Bacteria Species 0.000 abstract description 2
- 235000019441 ethanol Nutrition 0.000 description 18
- 230000000052 comparative effect Effects 0.000 description 14
- 230000000694 effects Effects 0.000 description 11
- 230000002147 killing effect Effects 0.000 description 11
- 230000002528 anti-freeze Effects 0.000 description 4
- LYCAIKOWRPUZTN-UHFFFAOYSA-N Ethylene glycol Chemical compound OCCO LYCAIKOWRPUZTN-UHFFFAOYSA-N 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 238000009472 formulation Methods 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 241000701161 unidentified adenovirus Species 0.000 description 3
- 241000700584 Simplexvirus Species 0.000 description 2
- 239000000654 additive Substances 0.000 description 2
- 230000015271 coagulation Effects 0.000 description 2
- 238000005345 coagulation Methods 0.000 description 2
- 238000002474 experimental method Methods 0.000 description 2
- 239000012530 fluid Substances 0.000 description 2
- 230000007774 longterm Effects 0.000 description 2
- 238000007711 solidification Methods 0.000 description 2
- 230000008023 solidification Effects 0.000 description 2
- 238000003860 storage Methods 0.000 description 2
- 230000004083 survival effect Effects 0.000 description 2
- 239000005708 Sodium hypochlorite Substances 0.000 description 1
- 230000009471 action Effects 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 150000001298 alcohols Chemical class 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000004027 cell Anatomy 0.000 description 1
- 230000030833 cell death Effects 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 239000003795 chemical substances by application Substances 0.000 description 1
- 230000000120 cytopathologic effect Effects 0.000 description 1
- 238000001514 detection method Methods 0.000 description 1
- 230000008014 freezing Effects 0.000 description 1
- 238000007710 freezing Methods 0.000 description 1
- 235000013611 frozen food Nutrition 0.000 description 1
- 238000007689 inspection Methods 0.000 description 1
- 230000003902 lesion Effects 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 230000002035 prolonged effect Effects 0.000 description 1
- 230000005855 radiation Effects 0.000 description 1
- SUKJFIGYRHOWBL-UHFFFAOYSA-N sodium hypochlorite Chemical compound [Na+].Cl[O-] SUKJFIGYRHOWBL-UHFFFAOYSA-N 0.000 description 1
- 239000007921 spray Substances 0.000 description 1
- 238000006467 substitution reaction Methods 0.000 description 1
- 241001529453 unidentified herpesvirus Species 0.000 description 1
- 210000003501 vero cell Anatomy 0.000 description 1
Classifications
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- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N31/00—Biocides, pest repellants or attractants, or plant growth regulators containing organic oxygen or sulfur compounds
- A01N31/02—Acyclic compounds
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01P—BIOCIDAL, PEST REPELLANT, PEST ATTRACTANT OR PLANT GROWTH REGULATORY ACTIVITY OF CHEMICAL COMPOUNDS OR PREPARATIONS
- A01P1/00—Disinfectants; Antimicrobial compounds or mixtures thereof
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2/00—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor
- A61L2/16—Methods or apparatus for disinfecting or sterilising materials or objects other than foodstuffs or contact lenses; Accessories therefor using chemical substances
- A61L2/22—Phase substances, e.g. smokes, aerosols or sprayed or atomised substances
-
- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
- A61L2101/00—Chemical composition of materials used in disinfecting, sterilising or deodorising
- A61L2101/32—Organic compounds
- A61L2101/34—Hydroxy compounds
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- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Zoology (AREA)
- General Health & Medical Sciences (AREA)
- General Chemical & Material Sciences (AREA)
- Pest Control & Pesticides (AREA)
- Plant Pathology (AREA)
- Wood Science & Technology (AREA)
- Engineering & Computer Science (AREA)
- Environmental Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Epidemiology (AREA)
- Animal Behavior & Ethology (AREA)
- Public Health (AREA)
- Veterinary Medicine (AREA)
- Agronomy & Crop Science (AREA)
- Dentistry (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
Abstract
The invention relates to the technical field of low-temperature disinfection, and in particular provides a low-temperature disinfectant for inactivating SARS-CoV-2 virus at-80 ℃. The low-temperature disinfectant at-80 ℃ comprises the following components: absolute ethanol, isopropanol and water. The invention provides a low-temperature disinfectant with simple components and no harm to human bodies and foods at-80 ℃, and the low-temperature disinfectant can keep a liquid state at-80 ℃. The low-temperature disinfectant with the temperature of minus 80 ℃ provided by the invention can effectively kill viruses and bacteria on the surface of objects in the environment of minus 80 ℃ and above, including novel coronavirus (SARS-CoV-2).
Description
Technical Field
The invention relates to the technical field of low-temperature disinfection, in particular to a low-temperature disinfectant for inactivating SARS-CoV-2 virus at-80 ℃.
Background
The low temperature environment of the cold chain food provides a good survival space for viruses, and the surface of the frozen food and the environment temperature of the cold storage are low, so that the cold chain food is suitable for long-term survival of viruses.
The existing low-temperature sterilization methods include radiation sterilization technology, ultrahigh pressure sterilization technology and the like. The former is not harmful to human body in principle, but the rays are not potentially dangerous to change the internal structure of food, and observation and experiments are yet to be confirmed. Whereas ultra-high pressure sterilization techniques have proven to have good low temperature sterilization effects, sterilization effects below zero are still in need of inspection. In addition, the manufacture of equipment by ultra-high pressure sterilization techniques is also a difficulty.
The 84 disinfectant commonly used in the prior art mainly comprises sodium hypochlorite, and is mainly used at normal temperature. When the temperature of the conventional 84 disinfectant is lower than zero, the conventional 84 disinfectant can freeze and solidify, and cannot exert a sterilizing effect. In normal temperature disinfectant, additive such as antifreezing agent is added to realize disinfection effect below zero degree.
However, when the low temperature is an ultra-low temperature of-80 ℃, the low temperature disinfectants such as-18 ℃, -20 ℃ and-40 ℃ on the market cannot achieve good disinfection effect. In the prior art, no disinfectant exists in a low-temperature environment of-80 ℃. There is no good disinfection technology for the surfaces of articles stored and preserved at-80 deg.c.
Disclosure of Invention
The invention fills the blank of the low-temperature disinfectant at-80 ℃ in the prior art, and provides the low-temperature disinfectant at-80 ℃ for inactivating SARS-CoV-2 virus.
The disinfectant provided by the invention comprises the following components: absolute ethanol, isopropanol and water.
In the disinfectant provided by the invention, the water accounts for 25-35% of the disinfectant by volume ratio.
In the disinfectant provided by the invention, the absolute ethyl alcohol accounts for 45-55% of the disinfectant by volume ratio.
In the disinfectant provided by the invention, the isopropyl alcohol accounts for 15-25% of the disinfectant by volume ratio.
In the disinfectant provided by the invention, the ratio of ethanol to isopropyl alcohol to water is (45-55)/(15-25)/(25-30) by volume ratio.
The use of the above-described sterilizing fluid for enhancing the sterilization efficiency in a low temperature environment is also claimed, as will be appreciated by those skilled in the art.
And the application of the disinfectant in low-temperature cold chain transportation for preventing novel coronavirus infection.
In the prior art, antifreeze or other additives are often added to conventional sterilizing fluids to achieve a subzero liquid state. However, the invention discovers that when the temperature is reduced to-80 ℃, if the phenomenon of solidification of the disinfectant is to be eliminated by adding the antifreeze, a large amount of the antifreeze is needed, and the addition of the antifreeze seriously reduces the sterilizing effect of the disinfectant at-80 ℃.
In the long-term research and trial, the invention unexpectedly finds that the ratio of the ethanol to the isopropanol to the water is (45-55): 15-25): 25-30, the ethanol can be in a liquid state at-80 ℃ and has good sterilization and disinfection effects.
Ethanol is a recognized product with rapid disinfection effect and is usually used in normal temperature environment. When the disinfectant is used in a low-temperature or ultra-low-temperature environment, not only the disinfectant effect but also the low-temperature or ultra-low-temperature environment needs to be considered. After proper ethanol and isopropanol are mixed according to a certain proportion, the freezing point of the disinfectant can be obviously reduced, the consumption of the ethanol can be reduced on the premise of having the same disinfection effect, and meanwhile, the disinfection action time can be prolonged
The invention also provides a method for sterilizing the low-temperature environment at-80 ℃, which uses the sterilizing liquid to spray and sterilize the low-temperature environment at-80 ℃.
In the method provided by the invention, the ratio of the consumption of the disinfectant to the area of the low-temperature environment to be disinfected is 1L:150-200m 2.
Pathogenic microorganisms such as viruses are relatively resistant to low temperature environments, and there is a need for sterilizing liquids capable of sterilizing articles in low temperature environments, such as cold chain transportation, cold storage warehouses, and the like.
The invention has the beneficial effects that:
(1) The invention provides a low-temperature disinfectant with simple components and no harm to human bodies and foods at-80 ℃, and the low-temperature disinfectant can keep a liquid state at-80 ℃.
(2) The low-temperature disinfectant with the temperature of minus 80 ℃ provided by the invention can effectively kill viruses and bacteria on the surface of objects in the environment of minus 80 ℃ and above, including novel coronavirus (SARS-CoV-2).
Detailed Description
For the purpose of making the objects, technical solutions and advantages of the present invention more apparent, the technical solutions of the present invention will be clearly and completely described below, and it is apparent that the described embodiments are some embodiments of the present invention, but not all embodiments. All other embodiments, which can be made by those skilled in the art based on the embodiments of the invention without making any inventive effort, are intended to be within the scope of the invention.
Example 1
The embodiment provides a low-temperature disinfectant with the temperature of-80 ℃, which comprises the following formula and preparation method: 55% of ethanol, 20% of isopropanol and 25% of water, and uniformly mixing.
Example 2
The embodiment provides a low-temperature disinfectant with the temperature of-80 ℃, which comprises the following formula and preparation method: 45% of ethanol, 25% of isopropanol and 30% of water, and uniformly mixing.
Example 3
The embodiment provides a low-temperature disinfectant with the temperature of-80 ℃, which comprises the following formula and preparation method: ethanol accounts for 50%, isopropanol accounts for 20%, water accounts for 30%, and the mixture is uniformly mixed.
Experimental example 1 optimal temperature detection of Low temperature disinfectant
The disinfection effect of the low-temperature disinfectant obtained in examples 1 to 3 at different temperatures was detected in this experimental example, and specifically, in this experimental example, the coagulation degree of each disinfectant at different temperatures, and the disinfection effect of the disinfectant were detected in detail, as shown in tables 1 and 2.
TABLE 1 degree of coagulation of Low temperature disinfectant solutions of examples 1-3
| Degree of solidification | Example 1 | Example 2 | Example 3 |
| Normal temperature | Does not solidify | Does not solidify | Does not solidify |
| -4℃ | Does not solidify | Does not solidify | Does not solidify |
| -20℃ | Does not solidify | Does not solidify | Does not solidify |
| -40℃ | Does not solidify | Does not solidify | Does not solidify |
| -60℃ | Does not solidify | Does not solidify | Does not solidify |
| -80℃ | Does not solidify | Does not solidify | Does not solidify |
TABLE 2 sterilizing effect of Low temperature sterilizing liquid of examples 1-3
| Sterilization and disinfection | Example 1 | Example 2 | Example 3 |
| Normal temperature | Effective and effective | Effective and effective | Effective and effective |
| -4℃ | Effective and effective | Effective and effective | Effective and effective |
| -20℃ | Effective and effective | Effective and effective | Effective and effective |
| -40℃ | Effective and effective | Effective and effective | Effective and effective |
| -60℃ | Effective and effective | Effective and effective | Effective and effective |
| -80℃ | Effective and effective | Effective and effective | Effective and effective |
The results show that the disinfectant obtained in the embodiment of the invention has the best disinfection and sterilization effects at the temperature of normal temperature to-80 ℃.
Experimental example 2 killing effect of Low-temperature disinfectant on different viruses
The experimental example evaluates the killing effect of the low-temperature disinfectant at-80 ℃ obtained in the example 1 on different viruses, and three viruses are selected for experiments, namely novel coronavirus (SARS-CoV-2), adenovirus and herpes simplex virus. The sensitive cells used for the virus are Vero, hep-2 and Vero cells.
The titer of the novel coronavirus used is 10 5.39 TCID50/0.1ml; adenovirus titer used was 10 7.74 TCID50/0.1ml; the herpes virus titres were 10 6.33 TCID50/0.1ml and the results are shown in tables 3, 4 and 5.
TABLE 3 killing effect of low temperature disinfectant on novel coronaviruses at-80 ℃ in refrigerator
TABLE 4 killing effect of low temperature disinfectant on adenovirus at-80 ℃ in refrigerator
TABLE 5 killing effect of low temperature disinfectant on herpes simplex virus at-80 ℃ in refrigerator
In the table, X represents cell death; + represents a cytopathic lesion; representing normal growth of cells
Comparative example 1 different alcohols
The low-temperature sterilizing liquid according to the present comparative example is different from example 1 in that ethylene glycol was used instead of isopropyl alcohol in the low-temperature sterilizing liquid a according to the present comparative example.
The killing effect of two low-temperature disinfectants on novel coronaviruses under the refrigerator-80 ℃ condition was tested by using the low-temperature disinfectant obtained in example 1 as C and adopting the same method as in experimental example 2, and is shown in Table 6.
Table 6 killing effect of low temperature disinfectant on novel coronavirus at-80 deg.c in refrigerator
The results of this comparative example demonstrate that only isopropanol works synergistically with ethanol.
Comparative example 2 different ethanol to isopropanol ratios
The low-temperature disinfectant provided by the comparative example is different from the low-temperature disinfectant D of the comparative example in that ethanol accounts for 40% of the low-temperature disinfectant, isopropanol accounts for 30% of the low-temperature disinfectant, and the ratio of ethanol to isopropanol is 40:30; in the low-temperature disinfectant E, ethanol accounts for 25% of the low-temperature disinfectant, isopropanol accounts for 45% of the low-temperature disinfectant, and the ratio of ethanol to isopropanol is 25:45.
The killing effect of three low-temperature sterilizing solutions on novel coronaviruses at-80 ℃ in a refrigerator was examined by using the low-temperature sterilizing solution obtained in example 1 as C in the same manner as in experimental example 2, and is shown in Table 7.
TABLE 7 killing effect of low temperature disinfectant on novel coronaviruses at-80 ℃ in refrigerator
The results of this comparative example demonstrate that ethanol and isopropanol only act synergistically at the appropriate ratio.
Comparative example 3 different Water content in disinfectant
The comparative example provides a low-temperature disinfectant, and in the low-temperature disinfectant F of the comparative example, water accounts for 40% -45% of the low-temperature disinfectant; in the low-temperature disinfectant G of the comparative example, water accounts for 35% -40% of the low-temperature disinfectant.
The killing effect of three low-temperature sterilizing solutions on novel coronaviruses at the temperature of-80 ℃ in a refrigerator was examined by using the low-temperature sterilizing solution obtained in example 1 as C and by using the same method as in experimental example 2, and is shown in Table 8.
TABLE 8 killing effect of low temperature disinfectant on novel coronaviruses at-80 ℃ in refrigerator
The results of this comparative example and comparative example 2 demonstrate that only water, ethanol and isopropanol in the low temperature sterilizing solution, which are suitable, act synergistically.
Finally, it should be noted that: the above embodiments are only for illustrating the technical solution of the present invention, and are not limiting; although the invention has been described in detail with reference to the foregoing embodiments, it will be understood by those of ordinary skill in the art that: the technical scheme described in the foregoing embodiments can be modified or some technical features thereof can be replaced by equivalents; such modifications and substitutions do not depart from the spirit and scope of the technical solutions of the embodiments of the present invention.
Claims (8)
1. The disinfectant is characterized in that ethanol accounts for 55% of the disinfectant, isopropanol accounts for 20% of the disinfectant, and water accounts for 25% of the disinfectant; or ethanol accounts for 45% of the disinfectant, isopropanol accounts for 25% of the disinfectant, and water accounts for 30% of the disinfectant; or ethanol accounts for 50% of the disinfectant, isopropanol accounts for 20% of the disinfectant, and water accounts for 30% of the disinfectant.
2. The disinfectant is applied to enhancing the disinfection and sterilization efficiency in a low-temperature environment, and consists of ethanol, isopropanol and water in a volume ratio of (45-55)/(15-25)/(25-35).
3. Use according to claim 2, characterized in that in the disinfectant, ethanol represents 55% of the disinfectant, isopropanol represents 20% of the disinfectant and water represents 25% of the disinfectant, by volume; or ethanol accounts for 45% of the disinfectant, isopropanol accounts for 25% of the disinfectant, and water accounts for 30% of the disinfectant; or ethanol accounts for 50% of the disinfectant, isopropanol accounts for 20% of the disinfectant, and water accounts for 30% of the disinfectant.
4. The application of the disinfectant in preventing the novel coronavirus infection in low-temperature cold chain transportation is that the disinfectant is composed of ethanol, isopropanol and water in the volume ratio of (45-55): (15-25): (25-35).
5. The use according to claim 4, wherein in the disinfectant, ethanol represents 55% of the disinfectant, isopropanol represents 20% of the disinfectant and water represents 25% of the disinfectant; or ethanol accounts for 45% of the disinfectant, isopropanol accounts for 25% of the disinfectant, and water accounts for 30% of the disinfectant; or ethanol accounts for 50% of the disinfectant, isopropanol accounts for 20% of the disinfectant, and water accounts for 30% of the disinfectant.
6. A method for sterilizing a low-temperature environment at-80 ℃, which is characterized in that a sterilizing liquid is used for spraying and sterilizing the low-temperature environment at-80 ℃; the disinfectant consists of ethanol, isopropanol and water in the volume ratio of (45-55) to (15-25) to (25-35).
7. The method of claim 6, wherein the sterilizing liquid comprises 55% ethanol, 20% isopropyl alcohol and 25% water by volume; or ethanol accounts for 45% of the disinfectant, isopropanol accounts for 25% of the disinfectant, and water accounts for 30% of the disinfectant; or ethanol accounts for 50% of the disinfectant, isopropanol accounts for 20% of the disinfectant, and water accounts for 30% of the disinfectant.
8. The method according to any one of claims 6 to 7, wherein the ratio of the amount of disinfectant to the area of the low temperature environment to be disinfected is 1L:150-200m 2.
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113133462A (en) * | 2021-04-28 | 2021-07-20 | 李俊 | Chlorine-containing disinfectant in low-temperature environment and preparation method thereof |
| CN113439741A (en) * | 2021-08-05 | 2021-09-28 | 湖北省农业科学院中药材研究所 | Preparation method of primary color soaked specimen of snake mushroom |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113133462A (en) * | 2021-04-28 | 2021-07-20 | 李俊 | Chlorine-containing disinfectant in low-temperature environment and preparation method thereof |
| CN113439741A (en) * | 2021-08-05 | 2021-09-28 | 湖北省农业科学院中药材研究所 | Preparation method of primary color soaked specimen of snake mushroom |
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