CN115466771A - Preparation method and equipment of deer blood polypeptide chelated calcium - Google Patents
Preparation method and equipment of deer blood polypeptide chelated calcium Download PDFInfo
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- CN115466771A CN115466771A CN202211248291.7A CN202211248291A CN115466771A CN 115466771 A CN115466771 A CN 115466771A CN 202211248291 A CN202211248291 A CN 202211248291A CN 115466771 A CN115466771 A CN 115466771A
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- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
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- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/107—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length by chemical modification of precursor peptides
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Abstract
The invention discloses a preparation method of deer blood polypeptide chelated calcium, which comprises the following specific steps: s1, processing fresh deer blood; s2, homogenizing deer blood tissues and carrying out ultrasonic crushing; s3, degrading deer blood by compounding a plurality of proteases; s4, fine filtering and spray drying of deer blood polypeptide; s5, preparing deer blood polypeptide chelated calcium; after preparation, the infrared detection of the deer blood polypeptide and the deer blood abortifacient chelated calcium and the determination of the composition of the deer blood polypeptide chelated calcium and amino acid are carried out; the invention also provides a preparation device of deer blood polypeptide chelated calcium, which comprises a preheating component and a spray drying tank; the preparation method comprises the following steps: ultrasonic crushing deer blood cells; degrading deer blood into deer blood polypeptide by compounding various proteases; spray drying deer blood polypeptide to obtain powdered deer blood polypeptide; dissolving deer blood polypeptide powder in distilled water, and mixing with calcium chloride solution to obtain deer blood polypeptide chelated calcium.
Description
Technical Field
The invention relates to the technical field of health care products, in particular to a preparation method and equipment of deer blood polypeptide chelated calcium.
Background
Sanguis Cervi, which is the bore blood or antler blood of Cervus Nippon Temminck or Cervus Elaphus of Cervidae, is hot, sweet and salty in taste, and enters liver and kidney meridians; has effects in nourishing blood, replenishing vital essence, promoting blood circulation, dispelling blood stasis, relieving swelling, and treating wound. In the existing animal blood, only one kind of deer blood is directly used for medical treatment, prevention and health care, but the deer blood products in China are mainly the direct and simple processing of the deer blood, the technical content of the products is lower, the deep processing products are few, the additional value of the products is low, and the nutritional value of the deer blood is not fully developed and utilized. Calcium is a mineral element essential for human body to maintain physiological functions, and can control metabolism, muscle contraction, growth and development, heart beating, cell division, brain thinking, blood coagulation, endocrine activity, etc.
Asians often do not receive adequate calcium supplementation from their daily diet due to lactose intolerance and dietary habits, and therefore calcium supplement formulations are often needed to meet calcium nutritional requirements. The polypeptide chelated calcium is a product formed by combining polypeptide and metal calcium ions through coordination covalent bonds, and has many advantages, such as fast absorption, strong nutrition, high biological value, and activities of oxidation resistance, antibiosis, blood fat reduction, immunoregulation and the like, so the polypeptide chelated calcium is increasingly a hot point for research at home and abroad.
Disclosure of Invention
The invention aims to provide a preparation method of deer blood polypeptide chelated calcium, which mainly comprises the steps of extracting deer blood polypeptide powder by taking sika deer blood as a raw material through an ultrasonic assisted extraction method and an enzymolysis method, and chelating the deer blood polypeptide powder with calcium chloride to obtain the deer blood polypeptide chelated calcium.
In order to achieve the purpose, the invention provides the following technical scheme:
a preparation method of deer blood polypeptide chelated calcium specifically comprises the following steps:
s1, processing fresh deer blood;
s2, homogenizing deer blood tissues and carrying out ultrasonic disruption;
s3, degrading deer blood by compounding a plurality of proteases, and specifically comprising the following steps:
(1) adding 4000ml of the deer blood polypeptide solution subjected to ultrasonic treatment into an enzymolysis tank, adjusting the temperature of the enzymolysis tank, and sequentially adding various proteases;
(2) firstly, regulating the temperature of an enzymolysis tank to be constant at 37 +/-1 ℃, adding neutral protease into the enzymolysis tank, wherein the enzyme addition amount is 9000 (U/g), adding phosphate solution to regulate the pH value to be 7, and carrying out enzymolysis for 4 hours; adjusting the temperature to 50 +/-3 ℃, continuously adjusting the pH value of the enzymolysis tank to 8 by using a phosphate solution, adding alkaline protease and papain with the enzyme addition amount of 9000 (U/g), preserving heat for enzymolysis for 5 hours, continuously adding 2mol/l NaOH solution for adjusting the pH value to =9, preserving heat for enzymolysis for 1 hour, and finishing enzymolysis;
(3) adjusting the pH value of the deer blood polypeptide solution subjected to enzymolysis to 7 by using 5g/L sorbic acid solution, and removing the activity of the added protease by using an ultrahigh-temperature instant sterilization device;
s4, fine filtering and spray drying of deer blood polypeptide;
s5, preparing deer blood polypeptide chelated calcium, dissolving deer blood polypeptide powder in distilled water, mixing with a calcium chloride solution until the concentration of deer blood polypeptide in a reaction system is 2.5mg/mL and the concentration of calcium ions is 5mmol/L, adjusting the pH value of the reaction system to 7.0 by using 0.05mol/LNaOH and 0.05mol/LHCl, and carrying out chelation reaction in a constant-temperature water bath shaker at 40 ℃ for 30min.
Preferably, the method for extracting the deer blood polypeptide by using an ultrasonic-assisted method comprises the following steps:
(1) the method comprises the following steps Adding 0.1% trisodium citrate into 2L of fresh sanguis Cervi, anticoagulating, centrifuging at 5000r/min for 30min, performing ultrasonic treatment for 2h to break membrane, centrifuging at 5000r/min for 15min, and freeze drying to obtain sanguis Cervi protein.
(2) The method comprises the following steps Dissolving 20g of deer blood protein in 200mL of deionized water, weighing a certain amount of protease, and carrying out enzymolysis for a certain time at a certain temperature and pH; and after enzymolysis is finished, inactivating enzyme for 10min, standing and cooling, centrifuging at 5000r/min for 15min, taking supernate, and freeze-drying for subsequent experiments.
Preferably, the infrared detection of the deer blood polypeptide and the deer blood abortifacient chelated calcium is as follows: mixing 2mg of deer blood polypeptide powder, deer blood polypeptide chelated calcium powder and 200mg of dried KBr in an agate mortar, grinding and tabletting; the sample was loaded into a Fourier transform Infrared Spectroscopy (FTIR) and its IR spectrum was measured at wavelengths of 4000-400 cm-1.
Preferably, the determination of the amino acid composition of the deer blood polypeptide chelated calcium comprises the following steps: accurately weighing 50mg of deer blood polypeptide chelated calcium freeze-dried sample into a hydrolysis tube, adding 10mL of 6mol/L HCl, adding 3-4 drops of phenol solution, putting the hydrolysis tube into a refrigerant, freezing for 3-5 min, vacuumizing, filling nitrogen, sealing, hydrolyzing for 22h in a constant-temperature air-blast drying box at 110 ℃, taking out and cooling, filtering the hydrolysate, washing the hydrolysis tube with deionized water for multiple times, transferring the hydrolysate into a 50mL volumetric flask, and fixing the volume.
Preferably, the determination is carried out according to the composition of the deer blood polypeptide chelated calcium amino acid, and the method also comprises the steps of taking 1mL of hydrolysate in a 5mL volumetric flask, drying at 40-50 ℃ by using a vacuum drier, dissolving the residue by using 1-2 mL of pure water, drying again, repeating for two times, finally evaporating to dryness, dissolving by using 1mL of buffer solution with pH2.2, and using for instrument determination.
Preferably, in the step S1, the processing of the fresh deer blood includes grinding the raw material blood by a colloid mill to obtain deer blood grinding fluid, or sequentially processing the raw material blood by vacuum freeze drying and low-temperature micro-grinding to obtain deer blood powder.
The invention also provides a preparation device of the deer blood polypeptide chelated calcium, which comprises a spray drying device, wherein the spray drying device comprises a preheating assembly and a spray drying tank, the preheating assembly comprises a preheating pipe and a preheating cylinder, a plurality of groups of positioning grooves are annularly and equidistantly formed in the inner wall of the preheating cylinder, the preheating pipe is fixedly clamped in the positioning grooves, a feed chute is formed in the top of the preheating cylinder, an inner groove of the feed chute is communicated with the preheating pipe, and the spray drying tank is fixedly installed at the bottom of the preheating cylinder;
the top of the preheating assembly is provided with a feeding assembly, and a grinding assembly is arranged in the feeding assembly.
Preferably, fixed mounting has the collecting chute to hold in the palm in the preheating section of thick bamboo, the collecting chute holds in the palm detachable and sets up in the preheating section of thick bamboo, the preheating section of thick bamboo inner wall is located the constant head tank position and is provided with electric heating pipe, spray drying jar inner wall is held in the palm the outer lane also to be coiled in the collecting chute and is provided with electric heating pipe.
Preferably, the feeding assembly comprises a hopper and a material pipe fixedly communicated with the bottom of the hopper, the grinding assembly comprises a support plate and a grinding wheel rotatably mounted at the bottom of the support plate, a discharging hole is formed in the support plate, a driving motor is mounted above the grinding wheel in a transmission manner, and the grinding surface of the grinding wheel is arranged in a gap with the inner surface of the feeding groove.
Preferably, preheating cylinder outer wall fixed mounting has the bull gear, collet upper surface one side is provided with the rotating electrical machines, the output shaft fixed mounting of rotating electrical machines has the pinion gear, bull gear and pinion gear meshing, spray drying tank bottoms face equidistant array has the universal wheel, universal wheel and collet upper surface laminating set up.
Compared with the prior art, the invention has the beneficial effects that:
the invention discloses a preparation method of deer blood polypeptide chelated calcium, which relates to a preparation method comprising the following steps: ultrasonic crushing deer blood cells; degrading deer blood into deer blood polypeptide by compounding various proteases; spray drying deer blood polypeptide to obtain powdered deer blood polypeptide; dissolving deer blood polypeptide powder in distilled water, and mixing with calcium chloride solution to obtain deer blood polypeptide chelated calcium;
according to the preparation equipment of the deer blood polypeptide chelated calcium, a plurality of inclined channels are formed on the inner wall of the preheating cylinder, so that the preheating time of the deer blood powder/crystal is prolonged, and the deer blood powder/crystal is obliquely arranged, so that the deer blood powder/crystal can smoothly enter the spray drying tank for continuous drying after being heated, and the energy-saving effect is achieved; furthermore, a plurality of parts of deer blood powder/crystal can be dried at one time, thereby achieving the purpose of efficiently producing deer blood crystal;
according to the preparation equipment of deer blood polypeptide chelated calcium, the grinding assembly is additionally arranged in the preheating link, the driving motor and the rotating motor act simultaneously, the raw materials in the previous sequence are agglomerated and ground for the second time and then are stirred into the preheating pipe, so that the raw materials can be fully preheated, the preheating effect is improved, and the feeding groove can smoothly enter the corresponding preheating pipe.
Drawings
FIG. 1 is a process for preparing deer blood polypeptide chelated calcium;
FIG. 2 is the red infrared spectrum of deer blood polypeptide and deer blood polypeptide chelated calcium in example 2;
FIG. 3 is a schematic view of the three-dimensional structure of the deer blood polypeptide chelated calcium preparation equipment
FIG. 4 is a schematic exploded perspective view of a spray drying apparatus according to the present invention;
FIG. 5 is a schematic perspective view of a spray drying apparatus according to the present invention;
FIG. 6 is a schematic perspective view of a spray drying canister of the present invention;
FIG. 7 is a schematic bottom perspective view of the feeding assembly and the polishing assembly of the present invention.
In the figure: 1. a preheating assembly; 101. a preheating pipe; 102. a preheating cylinder; 103. positioning a groove; 2. a spray drying tank; 3. a feed chute; 4. the material receiving groove supports; 5. an electric heating tube; 6. a feeding assembly; 601. a hopper; 602. a material pipe; 7. a grinding assembly; 701. a support plate; 702. grinding the wheel; 703. a blanking hole; 704. a drive motor; 8. a large gear ring; 9. a rotating electric machine; 10. a small gear ring.
Detailed Description
In order to make the objects, technical solutions and advantages of the present invention more apparent, the present invention is further described in detail with reference to the following embodiments. It should be understood that the specific embodiments described herein
The invention is to be considered as merely illustrative and not restrictive.
Example 1
As shown in fig. 1: a preparation method of deer blood polypeptide chelated calcium specifically comprises the following steps:
s1: taking 2L of fresh deer blood, adding 0.1% trisodium citrate for anticoagulation, centrifuging at 5000r/min for 30min, performing ultrasonic treatment for 2h to break membrane, centrifuging at 5000r/min for 15min, and freeze drying to obtain deer blood protein;
s2: 20g of deer blood protein is dissolved in 200mL of deionized water, and certain protease is weighed and subjected to enzymolysis for certain time at certain temperature and pH. After enzymolysis is finished, inactivating enzyme for 10min, standing and cooling, centrifuging at 5000r/min for 15min, taking supernate, and freeze-drying for subsequent experiments;
s3: adding 4000ml of the deer blood polypeptide solution subjected to ultrasonic treatment into an enzymolysis tank, adjusting the temperature of the enzymolysis tank to be constant at 37 ℃, adding 9000 (U/g) of neutral protease into the enzymolysis tank, adding phosphate solution to adjust the pH value to 7, and carrying out enzymolysis for 4 hours; adjusting the temperature to 53 ℃, continuously adjusting the pH value of the enzymolysis tank to 8 by using a phosphate solution, adding 9000 (U/g) alkaline protease and 9000 (U/g) papain, carrying out enzymolysis for 5 hours at constant temperature, continuously adding 2mol/L NaOH solution to adjust the pH value to =9, carrying out enzymolysis for 1 hour at constant temperature, and finishing the enzymolysis, wherein the enzymolysis conditions and the enzymolysis time are shown in Table 1. Adjusting the pH value of the deer blood polypeptide solution subjected to enzymolysis to 7 by using 5g/L sorbic acid solution, and removing the activity of the added protease by using an ultrahigh-temperature instantaneous sterilization device.
S4: carrying out high-speed spray centrifugal drying on the obtained raw material liquid, wherein the process parameters are as follows: the inlet temperature is 170 ℃, the outlet temperature is 70 ℃, the rotation speed of the atomizer is 3 ten thousand rpm, and the deer blood polypeptide dry powder with the water content below 10 percent is obtained.
S5: dissolving deer blood polypeptide powder in distilled water, mixing with calcium chloride solution until the concentration of deer blood polypeptide in the reaction system is 2.5mg/mL and the concentration of calcium ions is 5mmol/L, adjusting the pH value of the reaction system to 7.0 by using 0.05mol/LNaOH and 0.05mol/LHCl, and carrying out chelation reaction in a constant-temperature water bath shaker at 40 ℃ for 30min.
Specifically, in the step S1, the raw material blood is ground by a colloid mill to obtain the deer blood grinding fluid, or the raw material blood is sequentially processed by vacuum freeze drying and low-temperature micro-grinding to obtain the deer blood powder.
Specifically, the fine filtration of the deer blood polypeptide in the step S4 comprises adding hemoglobin powder and deer placenta powder into the micromolecular polysaccharide plant liquid, then adding water to 10-20 times of the solid, uniformly mixing the mixture to obtain a composite protein liquid, adding protease into the composite protein liquid, and carrying out enzymolysis on the composite protein liquid to obtain the micromolecular polypeptide primary liquid.
Specifically, the primary small molecule polypeptide liquid is cooled to 45-65 ℃, a plate-and-frame filter is used for filtering the primary small molecule polypeptide liquid, and filter residues are removed to obtain a fine polypeptide liquid.
Table 1: and (4) performing enzymolysis.
The invention also provides preparation equipment of the deer blood polypeptide chelated calcium, which comprises spray drying equipment, wherein the spray drying equipment comprises a preheating assembly 1, a spray drying tank 2 and a bottom support, the preheating assembly 1 comprises a preheating pipe 101 and a preheating cylinder 102, a plurality of groups of positioning grooves 103 are annularly and equidistantly formed in the inner wall of the preheating cylinder 102, the preheating pipe 101 is fixedly clamped in the positioning grooves 103, a feeding groove 3 is formed in the top of the preheating cylinder 102, an inner groove of the feeding groove 3 is communicated with the preheating pipe 101, and the spray drying tank 2 is fixedly arranged at the bottom of the preheating cylinder 102.
The receiving groove support 4 is fixedly installed in the preheating cylinder 102, the receiving groove support 4 is detachably arranged in the preheating cylinder 102, the electric heating pipe 5 is arranged on the position, located at the positioning groove 103, of the inner wall of the preheating cylinder 102, the electric heating pipe 5 is also spirally arranged on the outer ring of the receiving groove support 4 on the inner wall of the preheating cylinder 102, the drying equipment in the embodiment is simple in structure, a plurality of inclined channels are formed in the inner wall of the preheating cylinder 102, the preheating time of deer blood powder/crystals is prolonged, the deer blood powder/crystals are obliquely arranged, the deer blood powder/crystals can smoothly enter the spray drying tank 2 to be continuously dried after being heated, and the energy-saving effect is achieved; and multiple parts of deer blood powder/crystal can be dried at one time, so that the aim of efficiently producing the deer blood crystal is fulfilled;
at the same time, spray drying produces a dry powder with a moisture content of less than 5% and a water activity in the desired range of 0.05-0.30 at 25 ℃.
The top of the preheating assembly 1 is provided with a feeding assembly 6, the feeding assembly 6 is internally provided with a grinding assembly 7, the feeding assembly 6 comprises a hopper 601 and a material pipe 602 fixedly communicated with the bottom of the hopper 601, the grinding assembly 7 comprises a support plate 701 and a grinding wheel 702 rotatably mounted at the bottom of the support plate 701, a feeding hole 703 is formed in the support plate 701, a driving motor 704 is mounted above the grinding wheel 702 in a transmission manner, the grinding surface of the grinding wheel 702 is arranged in a gap with the inner surface of the feed chute 3, and colloid grinding raw materials are ground in the previous process and are discharged in a lump form, so before entering the preheating pipe 101, the grinding wheel 702 is additionally arranged, when the grinding raw materials enter the feed chute 3, the grinding materials are ground by the friction of the grinding wheel 702 and then are conveyed to the preheating pipe 101 through the inner groove of the feed chute 3 and the material hole formed in the preheating pipe 101, so that the raw materials are fully preheated, and the preheating effect is improved.
Example 2
As shown in fig. 1: the infrared detection of the deer blood polypeptide and the deer blood abortifacient chelated calcium is as follows: mixing 2mg of deer blood polypeptide powder, deer blood polypeptide chelated calcium powder and 200mg of dried KBr in an agate mortar, grinding and tabletting; the samples were loaded into a Fourier transform Infrared Spectroscopy (FTIR) and their IR spectra were measured at wavelengths of 4000-400 cm-1.
Table 2 amino acid types and contents.
Example 3
The determination of the composition of the deer blood polypeptide chelated calcium and amino acid comprises the following steps: accurately weighing 50mg of deer blood polypeptide chelated calcium freeze-dried sample into a hydrolysis tube, adding 10mL of 6mol/L HCl, adding 3-4 drops of phenol solution, putting the hydrolysis tube into a refrigerant, freezing for 3-5 min, vacuumizing, filling nitrogen, sealing, hydrolyzing for 22h in a constant-temperature air-blast drying box at 110 ℃, taking out and cooling, filtering the hydrolysate, washing the hydrolysis tube with deionized water for multiple times, transferring the hydrolysate into a 50mL volumetric flask, and fixing the volume.
Specifically, according to the determination of the composition of the deer blood polypeptide chelated calcium and amino acid, 1mL of hydrolysate is taken to be put into a 5mL volumetric flask, the volumetric flask is dried by a vacuum drier at the temperature of 40-50 ℃, residues are dissolved by 1-2 mL of pure water, the drying is carried out again and again, the drying is finally carried out, and the solution is dissolved by 1mL of buffer solution with the pH value of 2.2 and is used for the determination of an instrument.
Specifically, the method comprises accurately sucking 0.200mL of mixed amino acid standard, diluting to 5mL with buffer solution with pH of 2.2, wherein the concentration of the standard diluent is 5.00nmol/μ L, and determining the amino acid type and content of the sample by external standard method with automatic amino acid analyzer.
The above description is only for the preferred embodiment of the present invention, but the scope of the present invention is not limited thereto, and any person skilled in the art should be considered to be within the technical scope of the present invention, and the technical solutions and the inventive concepts thereof according to the present invention should be equivalent or changed within the scope of the present invention.
Claims (10)
1. A preparation method of deer blood polypeptide chelated calcium is characterized by comprising the following steps: the method specifically comprises the following steps:
s1, processing fresh deer blood;
s2, homogenizing deer blood tissues and carrying out ultrasonic disruption;
s3, degrading deer blood by compounding a plurality of proteases, and specifically comprising the following steps:
(1) adding 4000ml of the deer blood polypeptide solution subjected to ultrasonic treatment into an enzymolysis tank, adjusting the temperature of the enzymolysis tank, and sequentially adding various proteases;
(2) firstly, regulating the temperature of an enzymolysis tank to be constant at 37 +/-1 ℃, adding neutral protease into the enzymolysis tank, wherein the enzyme addition amount is 9000 (U/g), adding phosphate solution to regulate the pH value to be 7, and carrying out enzymolysis for 4 hours; adjusting the temperature to 50 +/-3 ℃, continuously adjusting the pH value of the enzymolysis tank to 8 by using a phosphate solution, adding alkaline protease and papain with the enzyme addition amount of 9000 (U/g), preserving heat for enzymolysis for 5 hours, continuously adding 2mol/l NaOH solution for adjusting the pH value to =9, preserving heat for enzymolysis for 1 hour, and finishing enzymolysis;
(3) adjusting the pH value of the deer blood polypeptide solution subjected to enzymolysis to 7 by using 5g/L sorbic acid solution, and removing the activity of the added protease by using an ultrahigh-temperature instant sterilization device;
s4, fine filtering and spray drying of deer blood polypeptide;
s5, preparing deer blood polypeptide chelated calcium, namely dissolving deer blood polypeptide powder in distilled water, mixing with a calcium chloride solution until the concentration of deer blood polypeptide in a reaction system is 2.5mg/mL and the concentration of calcium ions is 5mmol/L, adjusting the pH value of the reaction system to 7.0 by using 0.05mol/LNaOH and 0.05mol/LHCl, and carrying out chelation reaction in a constant-temperature water bath shaker at 40 ℃ for 30min.
2. The method for preparing deer blood polypeptide chelated calcium according to claim 1, characterized in that: the method for extracting the deer blood polypeptide by using an ultrasonic-assisted method comprises the following steps:
(1) the method comprises the following steps Taking 2L of fresh deer blood, adding 0.1% trisodium citrate for anticoagulation, centrifuging at 5000r/min for 30min, performing ultrasonic treatment for 2h to break membrane, centrifuging at 5000r/min for 15min, and freeze drying to obtain deer blood protein.
(2) The method comprises the following steps Dissolving 20g of deer blood protein in 200mL of deionized water, weighing a certain amount of protease, and carrying out enzymolysis for a certain time at a certain temperature and pH; and after enzymolysis is finished, inactivating enzyme for 10min, standing and cooling, centrifuging at 5000r/min for 15min, taking supernate, and freeze-drying for subsequent experiments.
3. The method for preparing deer blood polypeptide chelated calcium according to claim 1, characterized in that: the infrared detection of the deer blood polypeptide and deer blood abortifacient chelated calcium comprises the following steps: mixing 2mg of deer blood polypeptide powder, deer blood polypeptide chelated calcium powder and 200mg of dried KBr in an agate mortar, grinding and tabletting; the samples were loaded into a Fourier transform Infrared Spectroscopy (FTIR) and their IR spectra were measured at wavelengths of 4000-400 cm-1.
4. The method for preparing deer blood polypeptide chelated calcium according to claim 1, characterized in that: the determination of the composition of the deer blood polypeptide chelated calcium and amino acid comprises the following steps: accurately weighing 50mg of deer blood polypeptide chelated calcium freeze-dried sample into a hydrolysis tube, adding 10mL of 6mol/LHCl, adding 3-4 drops of phenol solution, putting the hydrolysis tube into a refrigerant, freezing for 3-5 min, vacuumizing, filling nitrogen, sealing, hydrolyzing for 22h in a constant-temperature air-blast drying box at 110 ℃, taking out and cooling, filtering the hydrolysate, washing the hydrolysis tube with deionized water for multiple times, transferring the hydrolysate into a 50mL volumetric flask, and fixing the volume.
5. The method for preparing deer blood polypeptide chelated calcium according to claim 4, wherein the steps of: according to the determination of the composition of the deer blood polypeptide chelated calcium and amino acid, 1mL of hydrolysate is taken to be put into a 5mL volumetric flask, dried by a vacuum drier at the temperature of 40-50 ℃, the residue is dissolved by 1-2 mL of pure water, and then dried and repeated twice, and finally evaporated to dryness, dissolved by 1mL of buffer solution with the pH value of 2.2 and used for the determination of instruments.
6. The method for preparing deer blood polypeptide chelated calcium according to claim 1, characterized in that: and (2) in the step (S1), the fresh deer blood is ground by using a colloid mill to obtain deer blood grinding fluid or the raw material blood is sequentially processed by using vacuum freeze drying and low-temperature micro-grinding technologies to obtain deer blood powder.
7. A preparation apparatus of deer blood polypeptide chelated calcium according to any one of claims 1-6, comprising a spray drying apparatus, characterized in that: the preheating device comprises a preheating assembly (1), a spray drying tank (2) and a bottom support, wherein the preheating assembly (1) comprises a preheating pipe (101) and a preheating cylinder (102), a plurality of groups of positioning grooves (103) are annularly and equidistantly formed in the inner wall of the preheating cylinder (102), the preheating pipe (101) is fixedly clamped in the positioning grooves (103), a feeding groove (3) is formed in the top of the preheating cylinder (102), an inner groove of the feeding groove (3) is communicated with the preheating pipe (101), and the spray drying tank (2) is fixedly installed at the bottom of the preheating cylinder (102);
the preheating assembly (1) is provided with a feeding assembly (6) at the top, and a grinding assembly (7) is arranged in the feeding assembly (6).
8. The apparatus for preparing deer blood polypeptide chelated calcium according to claim 7, wherein: the preheating cylinder (102) is internally and fixedly provided with a receiving groove support (4), the receiving groove support (4) is detachably arranged in the preheating cylinder (102), the inner wall of the preheating cylinder (102) is located at a positioning groove (103) and is provided with an electric heating pipe (5), and the inner wall of the spray drying tank (2) is provided with the electric heating pipe (5) in a spiral mode in the outer ring of the receiving groove support (4).
9. The apparatus for preparing deer blood polypeptide chelated calcium according to claim 7, wherein: the feeding assembly (6) comprises a hopper (601) and a material pipe (602) fixedly communicated with the bottom of the hopper (601), the grinding assembly (7) comprises a supporting plate (701) and a grinding wheel (702) rotatably installed at the bottom of the supporting plate (701), a discharging hole (703) is formed in the supporting plate (701), a driving motor (704) is installed above the grinding wheel (702) in a transmission mode, and the grinding surface of the grinding wheel (702) and the inner surface of the feeding groove (3) are arranged in a clearance mode.
10. The apparatus for preparing deer blood polypeptide chelated calcium according to claim 7, wherein: preheating section of thick bamboo (102) outer wall fixed mounting has bull gear (8), collet upper surface one side is provided with rotating electrical machines (9), the output shaft fixed mounting of rotating electrical machines (9) has little ring gear (10), bull gear (8) and little ring gear (10) meshing, spray drying jar (2) bottom surface equidistance array has the universal wheel, the laminating of universal wheel and collet upper surface sets up.
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Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199648A (en) * | 2011-03-25 | 2011-09-28 | 伊通满族自治县吉云鹿业发展有限公司 | Method for acquiring bioactive peptide by sequentially hydrolyzing deer haemproteins with two enzymes |
| CN102241733A (en) * | 2011-05-18 | 2011-11-16 | 吉林大学 | Deer ossein polypeptide chelated calcium and enteric capsule and preparation method of enteric capsule |
| CN103266156A (en) * | 2013-05-07 | 2013-08-28 | 黑龙江省野生动物研究所 | Method for preparing deer blood protein peptide by using fractional enzymolysis technology |
| CN107217082A (en) * | 2017-06-05 | 2017-09-29 | 深圳知本康业有限公司 | A kind of deer hemalbumin polypeptide and application |
| CN213557556U (en) * | 2020-10-16 | 2021-06-29 | 四川新渔现代生物技术有限公司 | Cactus crocus quick drying device |
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2022
- 2022-10-12 CN CN202211248291.7A patent/CN115466771A/en active Pending
Patent Citations (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102199648A (en) * | 2011-03-25 | 2011-09-28 | 伊通满族自治县吉云鹿业发展有限公司 | Method for acquiring bioactive peptide by sequentially hydrolyzing deer haemproteins with two enzymes |
| CN102241733A (en) * | 2011-05-18 | 2011-11-16 | 吉林大学 | Deer ossein polypeptide chelated calcium and enteric capsule and preparation method of enteric capsule |
| CN103266156A (en) * | 2013-05-07 | 2013-08-28 | 黑龙江省野生动物研究所 | Method for preparing deer blood protein peptide by using fractional enzymolysis technology |
| CN107217082A (en) * | 2017-06-05 | 2017-09-29 | 深圳知本康业有限公司 | A kind of deer hemalbumin polypeptide and application |
| CN213557556U (en) * | 2020-10-16 | 2021-06-29 | 四川新渔现代生物技术有限公司 | Cactus crocus quick drying device |
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